Splitting probabilities as a test of reaction coordinate choice in single-molecule experiments
Identifieur interne : 000261 ( Ncbi/Checkpoint ); précédent : 000260; suivant : 000262Splitting probabilities as a test of reaction coordinate choice in single-molecule experiments
Auteurs : John D. Chodera [États-Unis] ; Vijay S. Pande [États-Unis]Source :
- Physical review letters [ 0031-9007 ] ; 2011.
Abstract
To explain the observed dynamics in equilibrium single-molecule measurements of biomolecules, the experimental observable is often chosen as a putative reaction coordinate along which kinetic behavior is presumed to be governed by diffusive dynamics. Here, we invoke the splitting probability as a test of the suitability of such a proposed reaction coordinate. Comparison of the observed splitting probability with that computed from the kinetic model provides a simple test to reject poor reaction coordinates. We demonstrate this test for a force spectroscopy measurement of a DNA hairpin.
Url:
DOI: 10.1103/PhysRevLett.107.098102
PubMed: 21929272
PubMed Central: 4731355
Affiliations:
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