UV hyper‐resistance in Prochlorococcus MED4 results from a single base pair deletion just upstream of an operon encoding nudix hydrolase and photolyase
Identifieur interne : 000934 ( Main/Merge ); précédent : 000933; suivant : 000935UV hyper‐resistance in Prochlorococcus MED4 results from a single base pair deletion just upstream of an operon encoding nudix hydrolase and photolyase
Auteurs : Marcia S. Osburne [États-Unis] ; Brianne M. Holmbeck [États-Unis] ; Jorge Frias-Lopez [États-Unis] ; Robert Steen [États-Unis] ; Katherine Huang [États-Unis] ; Libusha Kelly [États-Unis] ; Allison Coe [États-Unis] ; Kristin Waraska [États-Unis] ; Andrew Gagne [États-Unis] ; Sallie W. Chisholm [États-Unis]Source :
- Environmental Microbiology [ 1462-2912 ] ; 2010-07.
Abstract
Exposure to solar radiation can cause mortality in natural communities of pico‐phytoplankton, both at the surface and to a depth of at least 30 m. DNA damage is a significant cause of death, mainly due to cyclobutane pyrimidine dimer formation, which can be lethal if not repaired. While developing a UV mutagenesis protocol for the marine cyanobacterium Prochlorococcus, we isolated a UV‐hyper‐resistant variant of high light‐adapted strain MED4. The hyper‐resistant strain was constitutively upregulated for expression of the mutT‐phrB operon, encoding nudix hydrolase and photolyase, both of which are involved in repair of DNA damage that can be caused by UV light. Photolyase (PhrB) breaks pyrimidine dimers typically caused by UV exposure, using energy from visible light in the process known as photoreactivation. Nudix hydrolase (MutT) hydrolyses 8‐oxo‐dGTP, an aberrant form of GTP that results from oxidizing conditions, including UV radiation, thus impeding mispairing and mutagenesis by preventing incorporation of the aberrant form into DNA. These processes are error‐free, in contrast to error‐prone SOS dark repair systems that are widespread in bacteria. The UV‐hyper‐resistant strain contained only a single mutation: a 1 bp deletion in the intergenic region directly upstream of the mutT‐phrB operon. Two subsequent enrichments for MED4 UV‐hyper‐resistant strains from MED4 wild‐type cultures gave rise to strains containing this same 1 bp deletion, affirming its connection to the hyper‐resistant phenotype. These results have implications for Prochlorococcus DNA repair mechanisms, genome stability and possibly lysogeny.
Url:
- https://api.istex.fr/document/A16CC2963028CA70B351BB49500BE39C9C7E1A8E/fulltext/pdf
- http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2955971
DOI: 10.1111/j.1462-2920.2010.02203.x
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Links to Exploration step
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<front><div type="abstract" xml:lang="en">Exposure to solar radiation can cause mortality in natural communities of pico‐phytoplankton, both at the surface and to a depth of at least 30 m. DNA damage is a significant cause of death, mainly due to cyclobutane pyrimidine dimer formation, which can be lethal if not repaired. While developing a UV mutagenesis protocol for the marine cyanobacterium Prochlorococcus, we isolated a UV‐hyper‐resistant variant of high light‐adapted strain MED4. The hyper‐resistant strain was constitutively upregulated for expression of the mutT‐phrB operon, encoding nudix hydrolase and photolyase, both of which are involved in repair of DNA damage that can be caused by UV light. Photolyase (PhrB) breaks pyrimidine dimers typically caused by UV exposure, using energy from visible light in the process known as photoreactivation. Nudix hydrolase (MutT) hydrolyses 8‐oxo‐dGTP, an aberrant form of GTP that results from oxidizing conditions, including UV radiation, thus impeding mispairing and mutagenesis by preventing incorporation of the aberrant form into DNA. These processes are error‐free, in contrast to error‐prone SOS dark repair systems that are widespread in bacteria. The UV‐hyper‐resistant strain contained only a single mutation: a 1 bp deletion in the intergenic region directly upstream of the mutT‐phrB operon. Two subsequent enrichments for MED4 UV‐hyper‐resistant strains from MED4 wild‐type cultures gave rise to strains containing this same 1 bp deletion, affirming its connection to the hyper‐resistant phenotype. These results have implications for Prochlorococcus DNA repair mechanisms, genome stability and possibly lysogeny.</div>
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<author><name sortKey="Holmbeck, Brianne M" sort="Holmbeck, Brianne M" uniqKey="Holmbeck B" first="Brianne M" last="Holmbeck">Brianne M. Holmbeck</name>
<affiliation wicri:level="2"><nlm:aff id="au1"><institution>Department of Civil and Environmental Engineering, Massachusetts Institute of Technology</institution>
<addr-line>Cambridge, MA 02139, USA</addr-line>
</nlm:aff>
<country xml:lang="fr">États-Unis</country>
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<placeName><region type="state">Massachusetts</region>
</placeName>
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</author>
<author><name sortKey="Frias Lopez, Jorge" sort="Frias Lopez, Jorge" uniqKey="Frias Lopez J" first="Jorge" last="Frias-Lopez">Jorge Frias-Lopez</name>
<affiliation wicri:level="2"><nlm:aff id="au1"><institution>Department of Civil and Environmental Engineering, Massachusetts Institute of Technology</institution>
<addr-line>Cambridge, MA 02139, USA</addr-line>
</nlm:aff>
<country xml:lang="fr">États-Unis</country>
<wicri:regionArea>Cambridge, MA 02139</wicri:regionArea>
<placeName><region type="state">Massachusetts</region>
</placeName>
</affiliation>
</author>
<author><name sortKey="Steen, Robert" sort="Steen, Robert" uniqKey="Steen R" first="Robert" last="Steen">Robert Steen</name>
<affiliation wicri:level="2"><nlm:aff id="au2"><institution>Biopolymers Facility, Harvard Medical School</institution>
<addr-line>Boston, MA 02115, USA</addr-line>
</nlm:aff>
<country xml:lang="fr">États-Unis</country>
<wicri:regionArea>Boston, MA 02115</wicri:regionArea>
<placeName><region type="state">Massachusetts</region>
</placeName>
</affiliation>
</author>
<author><name sortKey="Huang, Katherine" sort="Huang, Katherine" uniqKey="Huang K" first="Katherine" last="Huang">Katherine Huang</name>
<affiliation wicri:level="2"><nlm:aff id="au1"><institution>Department of Civil and Environmental Engineering, Massachusetts Institute of Technology</institution>
<addr-line>Cambridge, MA 02139, USA</addr-line>
</nlm:aff>
<country xml:lang="fr">États-Unis</country>
<wicri:regionArea>Cambridge, MA 02139</wicri:regionArea>
<placeName><region type="state">Massachusetts</region>
</placeName>
</affiliation>
</author>
<author><name sortKey="Kelly, Libusha" sort="Kelly, Libusha" uniqKey="Kelly L" first="Libusha" last="Kelly">Libusha Kelly</name>
<affiliation wicri:level="2"><nlm:aff id="au1"><institution>Department of Civil and Environmental Engineering, Massachusetts Institute of Technology</institution>
<addr-line>Cambridge, MA 02139, USA</addr-line>
</nlm:aff>
<country xml:lang="fr">États-Unis</country>
<wicri:regionArea>Cambridge, MA 02139</wicri:regionArea>
<placeName><region type="state">Massachusetts</region>
</placeName>
</affiliation>
</author>
<author><name sortKey="Coe, Allison" sort="Coe, Allison" uniqKey="Coe A" first="Allison" last="Coe">Allison Coe</name>
<affiliation wicri:level="2"><nlm:aff id="au1"><institution>Department of Civil and Environmental Engineering, Massachusetts Institute of Technology</institution>
<addr-line>Cambridge, MA 02139, USA</addr-line>
</nlm:aff>
<country xml:lang="fr">États-Unis</country>
<wicri:regionArea>Cambridge, MA 02139</wicri:regionArea>
<placeName><region type="state">Massachusetts</region>
</placeName>
</affiliation>
</author>
<author><name sortKey="Waraska, Kristin" sort="Waraska, Kristin" uniqKey="Waraska K" first="Kristin" last="Waraska">Kristin Waraska</name>
<affiliation wicri:level="2"><nlm:aff id="au2"><institution>Biopolymers Facility, Harvard Medical School</institution>
<addr-line>Boston, MA 02115, USA</addr-line>
</nlm:aff>
<country xml:lang="fr">États-Unis</country>
<wicri:regionArea>Boston, MA 02115</wicri:regionArea>
<placeName><region type="state">Massachusetts</region>
</placeName>
</affiliation>
</author>
<author><name sortKey="Gagne, Andrew" sort="Gagne, Andrew" uniqKey="Gagne A" first="Andrew" last="Gagne">Andrew Gagne</name>
<affiliation wicri:level="2"><nlm:aff id="au2"><institution>Biopolymers Facility, Harvard Medical School</institution>
<addr-line>Boston, MA 02115, USA</addr-line>
</nlm:aff>
<country xml:lang="fr">États-Unis</country>
<wicri:regionArea>Boston, MA 02115</wicri:regionArea>
<placeName><region type="state">Massachusetts</region>
</placeName>
</affiliation>
</author>
<author><name sortKey="Chisholm, Sallie W" sort="Chisholm, Sallie W" uniqKey="Chisholm S" first="Sallie W" last="Chisholm">Sallie W. Chisholm</name>
<affiliation wicri:level="2"><nlm:aff id="au1"><institution>Department of Civil and Environmental Engineering, Massachusetts Institute of Technology</institution>
<addr-line>Cambridge, MA 02139, USA</addr-line>
</nlm:aff>
<country xml:lang="fr">États-Unis</country>
<wicri:regionArea>Cambridge, MA 02139</wicri:regionArea>
<placeName><region type="state">Massachusetts</region>
</placeName>
</affiliation>
</author>
</titleStmt>
<publicationStmt><idno type="wicri:source">PMC</idno>
<idno type="pmid">20345942</idno>
<idno type="pmc">2955971</idno>
<idno type="url">http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2955971</idno>
<idno type="RBID">PMC:2955971</idno>
<idno type="doi">10.1111/j.1462-2920.2010.02203.x</idno>
<date when="2010">2010</date>
<idno type="wicri:Area/Pmc/Corpus">000525</idno>
<idno type="wicri:Area/Pmc/Curation">000525</idno>
<idno type="wicri:Area/Pmc/Checkpoint">000542</idno>
<idno type="wicri:Area/Ncbi/Merge">000128</idno>
<idno type="wicri:Area/Ncbi/Curation">000128</idno>
<idno type="wicri:Area/Ncbi/Checkpoint">000128</idno>
<idno type="wicri:doubleKey">1462-2912:2010:Osburne M:uv:hyper:resistance</idno>
</publicationStmt>
<sourceDesc><biblStruct><analytic><title xml:lang="en" level="a" type="main">UV hyper-resistance in <italic>Prochlorococcus</italic>
MED4 results from a single base pair deletion just upstream of an operon encoding nudix hydrolase and photolyase</title>
<author><name sortKey="Osburne, Marcia S" sort="Osburne, Marcia S" uniqKey="Osburne M" first="Marcia S" last="Osburne">Marcia S. Osburne</name>
<affiliation wicri:level="2"><nlm:aff id="au1"><institution>Department of Civil and Environmental Engineering, Massachusetts Institute of Technology</institution>
<addr-line>Cambridge, MA 02139, USA</addr-line>
</nlm:aff>
<country xml:lang="fr">États-Unis</country>
<wicri:regionArea>Cambridge, MA 02139</wicri:regionArea>
<placeName><region type="state">Massachusetts</region>
</placeName>
</affiliation>
</author>
<author><name sortKey="Holmbeck, Brianne M" sort="Holmbeck, Brianne M" uniqKey="Holmbeck B" first="Brianne M" last="Holmbeck">Brianne M. Holmbeck</name>
<affiliation wicri:level="2"><nlm:aff id="au1"><institution>Department of Civil and Environmental Engineering, Massachusetts Institute of Technology</institution>
<addr-line>Cambridge, MA 02139, USA</addr-line>
</nlm:aff>
<country xml:lang="fr">États-Unis</country>
<wicri:regionArea>Cambridge, MA 02139</wicri:regionArea>
<placeName><region type="state">Massachusetts</region>
</placeName>
</affiliation>
</author>
<author><name sortKey="Frias Lopez, Jorge" sort="Frias Lopez, Jorge" uniqKey="Frias Lopez J" first="Jorge" last="Frias-Lopez">Jorge Frias-Lopez</name>
<affiliation wicri:level="2"><nlm:aff id="au1"><institution>Department of Civil and Environmental Engineering, Massachusetts Institute of Technology</institution>
<addr-line>Cambridge, MA 02139, USA</addr-line>
</nlm:aff>
<country xml:lang="fr">États-Unis</country>
<wicri:regionArea>Cambridge, MA 02139</wicri:regionArea>
<placeName><region type="state">Massachusetts</region>
</placeName>
</affiliation>
</author>
<author><name sortKey="Steen, Robert" sort="Steen, Robert" uniqKey="Steen R" first="Robert" last="Steen">Robert Steen</name>
<affiliation wicri:level="2"><nlm:aff id="au2"><institution>Biopolymers Facility, Harvard Medical School</institution>
<addr-line>Boston, MA 02115, USA</addr-line>
</nlm:aff>
<country xml:lang="fr">États-Unis</country>
<wicri:regionArea>Boston, MA 02115</wicri:regionArea>
<placeName><region type="state">Massachusetts</region>
</placeName>
</affiliation>
</author>
<author><name sortKey="Huang, Katherine" sort="Huang, Katherine" uniqKey="Huang K" first="Katherine" last="Huang">Katherine Huang</name>
<affiliation wicri:level="2"><nlm:aff id="au1"><institution>Department of Civil and Environmental Engineering, Massachusetts Institute of Technology</institution>
<addr-line>Cambridge, MA 02139, USA</addr-line>
</nlm:aff>
<country xml:lang="fr">États-Unis</country>
<wicri:regionArea>Cambridge, MA 02139</wicri:regionArea>
<placeName><region type="state">Massachusetts</region>
</placeName>
</affiliation>
</author>
<author><name sortKey="Kelly, Libusha" sort="Kelly, Libusha" uniqKey="Kelly L" first="Libusha" last="Kelly">Libusha Kelly</name>
<affiliation wicri:level="2"><nlm:aff id="au1"><institution>Department of Civil and Environmental Engineering, Massachusetts Institute of Technology</institution>
<addr-line>Cambridge, MA 02139, USA</addr-line>
</nlm:aff>
<country xml:lang="fr">États-Unis</country>
<wicri:regionArea>Cambridge, MA 02139</wicri:regionArea>
<placeName><region type="state">Massachusetts</region>
</placeName>
</affiliation>
</author>
<author><name sortKey="Coe, Allison" sort="Coe, Allison" uniqKey="Coe A" first="Allison" last="Coe">Allison Coe</name>
<affiliation wicri:level="2"><nlm:aff id="au1"><institution>Department of Civil and Environmental Engineering, Massachusetts Institute of Technology</institution>
<addr-line>Cambridge, MA 02139, USA</addr-line>
</nlm:aff>
<country xml:lang="fr">États-Unis</country>
<wicri:regionArea>Cambridge, MA 02139</wicri:regionArea>
<placeName><region type="state">Massachusetts</region>
</placeName>
</affiliation>
</author>
<author><name sortKey="Waraska, Kristin" sort="Waraska, Kristin" uniqKey="Waraska K" first="Kristin" last="Waraska">Kristin Waraska</name>
<affiliation wicri:level="2"><nlm:aff id="au2"><institution>Biopolymers Facility, Harvard Medical School</institution>
<addr-line>Boston, MA 02115, USA</addr-line>
</nlm:aff>
<country xml:lang="fr">États-Unis</country>
<wicri:regionArea>Boston, MA 02115</wicri:regionArea>
<placeName><region type="state">Massachusetts</region>
</placeName>
</affiliation>
</author>
<author><name sortKey="Gagne, Andrew" sort="Gagne, Andrew" uniqKey="Gagne A" first="Andrew" last="Gagne">Andrew Gagne</name>
<affiliation wicri:level="2"><nlm:aff id="au2"><institution>Biopolymers Facility, Harvard Medical School</institution>
<addr-line>Boston, MA 02115, USA</addr-line>
</nlm:aff>
<country xml:lang="fr">États-Unis</country>
<wicri:regionArea>Boston, MA 02115</wicri:regionArea>
<placeName><region type="state">Massachusetts</region>
</placeName>
</affiliation>
</author>
<author><name sortKey="Chisholm, Sallie W" sort="Chisholm, Sallie W" uniqKey="Chisholm S" first="Sallie W" last="Chisholm">Sallie W. Chisholm</name>
<affiliation wicri:level="2"><nlm:aff id="au1"><institution>Department of Civil and Environmental Engineering, Massachusetts Institute of Technology</institution>
<addr-line>Cambridge, MA 02139, USA</addr-line>
</nlm:aff>
<country xml:lang="fr">États-Unis</country>
<wicri:regionArea>Cambridge, MA 02139</wicri:regionArea>
<placeName><region type="state">Massachusetts</region>
</placeName>
</affiliation>
</author>
</analytic>
<series><title level="j">Environmental Microbiology</title>
<idno type="ISSN">1462-2912</idno>
<idno type="eISSN">1462-2920</idno>
<imprint><date when="2010">2010</date>
</imprint>
</series>
</biblStruct>
</sourceDesc>
</fileDesc>
<profileDesc><textClass></textClass>
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</teiHeader>
<front><div type="abstract" xml:lang="en"><p>Exposure to solar radiation can cause mortality in natural communities of pico-phytoplankton, both at the surface and to a depth of at least 30 m. DNA damage is a significant cause of death, mainly due to cyclobutane pyrimidine dimer formation, which can be lethal if not repaired. While developing a UV mutagenesis protocol for the marine cyanobacterium <italic>Prochlorococcus</italic>
, we isolated a UV-hyper-resistant variant of high light-adapted strain MED4. The hyper-resistant strain was constitutively upregulated for expression of the <italic>mutT</italic>
-<italic>phrB</italic>
operon, encoding nudix hydrolase and photolyase, both of which are involved in repair of DNA damage that can be caused by UV light. Photolyase (PhrB) breaks pyrimidine dimers typically caused by UV exposure, using energy from visible light in the process known as photoreactivation. Nudix hydrolase (MutT) hydrolyses 8-oxo-dGTP, an aberrant form of GTP that results from oxidizing conditions, including UV radiation, thus impeding mispairing and mutagenesis by preventing incorporation of the aberrant form into DNA. These processes are error-free, in contrast to error-prone SOS dark repair systems that are widespread in bacteria. The UV-hyper-resistant strain contained only a single mutation: a 1 bp deletion in the intergenic region directly upstream of the <italic>mutT</italic>
-<italic>phrB</italic>
operon. Two subsequent enrichments for MED4 UV-hyper-resistant strains from MED4 wild-type cultures gave rise to strains containing this same 1 bp deletion, affirming its connection to the hyper-resistant phenotype. These results have implications for <italic>Prochlorococcus</italic>
DNA repair mechanisms, genome stability and possibly lysogeny.</p>
</div>
</front>
<back><div1 type="bibliography"><listBibl><biblStruct><analytic><author><name sortKey="Agusti, S" uniqKey="Agusti S">S Agustí</name>
</author>
<author><name sortKey="Llabres, M" uniqKey="Llabres M">M Llabrés</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Alcantara Diaz, D" uniqKey="Alcantara Diaz D">D Alcantara-Diaz</name>
</author>
<author><name sortKey="Brena Valle, M" uniqKey="Brena Valle M">M Brena-Valle</name>
</author>
<author><name sortKey="Serment Guerrero, J" uniqKey="Serment Guerrero J">J Serment-Guerrero</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Beam, C" uniqKey="Beam C">C Beam</name>
</author>
<author><name sortKey="Saveson, C" uniqKey="Saveson C">C Saveson</name>
</author>
<author><name sortKey="Lovett, S" uniqKey="Lovett S">S Lovett</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Bolstad, B" uniqKey="Bolstad B">B Bolstad</name>
</author>
<author><name sortKey="Irizarry, R" uniqKey="Irizarry R">R Irizarry</name>
</author>
<author><name sortKey="Astrand, M" uniqKey="Astrand M">M Astrand</name>
</author>
<author><name sortKey="Speed, T" uniqKey="Speed T">T Speed</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Boubriak, I" uniqKey="Boubriak I">I Boubriak</name>
</author>
<author><name sortKey="Ng, W" uniqKey="Ng W">W Ng</name>
</author>
<author><name sortKey="Dassarma, Ps" uniqKey="Dassarma P">PS Dassarma</name>
</author>
<author><name sortKey="Dassar, A" uniqKey="Dassar A">A Dassar</name>
</author>
<author><name sortKey="Crowley, D" uniqKey="Crowley D">D Crowley</name>
</author>
<author><name sortKey="Mccready, S" uniqKey="Mccready S">S McCready</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Buma, A" uniqKey="Buma A">A Buma</name>
</author>
<author><name sortKey="Helbling, E" uniqKey="Helbling E">E Helbling</name>
</author>
<author><name sortKey="Boer, Md" uniqKey="Boer M">MD Boer</name>
</author>
<author><name sortKey="Villafa E, V" uniqKey="Villafa E V">V Villafañe</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Christaki, U" uniqKey="Christaki U">U Christaki</name>
</author>
<author><name sortKey="Courties, C" uniqKey="Courties C">C Courties</name>
</author>
<author><name sortKey="Karayanni, H" uniqKey="Karayanni H">H Karayanni</name>
</author>
<author><name sortKey="Giannakourou, A" uniqKey="Giannakourou A">A Giannakourou</name>
</author>
<author><name sortKey="Maravelias, C" uniqKey="Maravelias C">C Maravelias</name>
</author>
<author><name sortKey="Kormas, K" uniqKey="Kormas K">K Kormas</name>
</author>
<author><name sortKey="Lebaron, P" uniqKey="Lebaron P">P Lebaron</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Coleman, M" uniqKey="Coleman M">M Coleman</name>
</author>
<author><name sortKey="Chisholm, S" uniqKey="Chisholm S">S Chisholm</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Cox, M" uniqKey="Cox M">M Cox</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Delong, E" uniqKey="Delong E">E DeLong</name>
</author>
<author><name sortKey="Preston, C" uniqKey="Preston C">C Preston</name>
</author>
<author><name sortKey="Mincer, T" uniqKey="Mincer T">T Mincer</name>
</author>
<author><name sortKey="Rich, V" uniqKey="Rich V">V Rich</name>
</author>
<author><name sortKey="Hallam, S" uniqKey="Hallam S">S Hallam</name>
</author>
<author><name sortKey="Frigaard, N" uniqKey="Frigaard N">N Frigaard</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Frias Lopez, J" uniqKey="Frias Lopez J">J Frias-Lopez</name>
</author>
<author><name sortKey="Shi, Y" uniqKey="Shi Y">Y Shi</name>
</author>
<author><name sortKey="Tyson, G" uniqKey="Tyson G">G Tyson</name>
</author>
<author><name sortKey="Coleman, M" uniqKey="Coleman M">M Coleman</name>
</author>
<author><name sortKey="Schuster, S" uniqKey="Schuster S">S Schuster</name>
</author>
<author><name sortKey="Chisholm, S" uniqKey="Chisholm S">S Chisholm</name>
</author>
<author><name sortKey="Delong, E" uniqKey="Delong E">E Delong</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Guillou, L" uniqKey="Guillou L">L Guillou</name>
</author>
<author><name sortKey="Jacquet, S" uniqKey="Jacquet S">S Jacquet</name>
</author>
<author><name sortKey="Chretiennot Dinet, M J" uniqKey="Chretiennot Dinet M">M-J Chretiennot-Dinet</name>
</author>
<author><name sortKey="Vaulot, D" uniqKey="Vaulot D">D Vaulot</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Karlin, S" uniqKey="Karlin S">S Karlin</name>
</author>
<author><name sortKey="Brocchiere, L" uniqKey="Brocchiere L">L Brocchiere</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Kettler, G" uniqKey="Kettler G">G Kettler</name>
</author>
<author><name sortKey="Martiny, A" uniqKey="Martiny A">A Martiny</name>
</author>
<author><name sortKey="Huang, K" uniqKey="Huang K">K Huang</name>
</author>
<author><name sortKey="Zucker, J" uniqKey="Zucker J">J Zucker</name>
</author>
<author><name sortKey="Coleman, M" uniqKey="Coleman M">M Coleman</name>
</author>
<author><name sortKey="Rodrigue, S" uniqKey="Rodrigue S">S Rodrigue</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Kidambi, S" uniqKey="Kidambi S">S Kidambi</name>
</author>
<author><name sortKey="Booth, M" uniqKey="Booth M">M Booth</name>
</author>
<author><name sortKey="Kokjohn, T" uniqKey="Kokjohn T">T Kokjohn</name>
</author>
<author><name sortKey="Miller, R" uniqKey="Miller R">R Miller</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Latifi, A" uniqKey="Latifi A">A Latifi</name>
</author>
<author><name sortKey="Ruiz, M" uniqKey="Ruiz M">M Ruiz</name>
</author>
<author><name sortKey="Zhang, C" uniqKey="Zhang C">C Zhang</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Lindell, D" uniqKey="Lindell D">D Lindell</name>
</author>
<author><name sortKey="Jaffe, J" uniqKey="Jaffe J">J Jaffe</name>
</author>
<author><name sortKey="Johnson, Z" uniqKey="Johnson Z">Z Johnson</name>
</author>
<author><name sortKey="Church, G" uniqKey="Church G">G Church</name>
</author>
<author><name sortKey="Chisholm, S" uniqKey="Chisholm S">S Chisholm</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Livak, K" uniqKey="Livak K">K Livak</name>
</author>
<author><name sortKey="Schmittgem, T" uniqKey="Schmittgem T">T Schmittgem</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Llabres, M" uniqKey="Llabres M">M Llabrés</name>
</author>
<author><name sortKey="Agusti, S" uniqKey="Agusti S">S Agustí</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Michel, B" uniqKey="Michel B">B Michel</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Minda, R" uniqKey="Minda R">R Minda</name>
</author>
<author><name sortKey="Ramchandani, J" uniqKey="Ramchandani J">J Ramchandani</name>
</author>
<author><name sortKey="Joshi, V" uniqKey="Joshi V">V Joshi</name>
</author>
<author><name sortKey="Bhattacharjee, S" uniqKey="Bhattacharjee S">S Bhattacharjee</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Mira, A" uniqKey="Mira A">A Mira</name>
</author>
<author><name sortKey="Ochman, H" uniqKey="Ochman H">H Ochman</name>
</author>
<author><name sortKey="Moran, N" uniqKey="Moran N">N Moran</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Moore, L" uniqKey="Moore L">L Moore</name>
</author>
<author><name sortKey="Coe, A" uniqKey="Coe A">A Coe</name>
</author>
<author><name sortKey="Zinser, E" uniqKey="Zinser E">E Zinser</name>
</author>
<author><name sortKey="Saito, M" uniqKey="Saito M">M Saito</name>
</author>
<author><name sortKey="Sullivan, M" uniqKey="Sullivan M">M Sullivan</name>
</author>
<author><name sortKey="Lindell, D" uniqKey="Lindell D">D Lindell</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Napolitano, R" uniqKey="Napolitano R">R Napolitano</name>
</author>
<author><name sortKey="Janel Bintz, R" uniqKey="Janel Bintz R">R Janel-Bintz</name>
</author>
<author><name sortKey="Wagner, J" uniqKey="Wagner J">J Wagner</name>
</author>
<author><name sortKey="Fuchs, R" uniqKey="Fuchs R">R Fuchs</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Ng, W" uniqKey="Ng W">W Ng</name>
</author>
<author><name sortKey="Pakrasi, H" uniqKey="Pakrasi H">H Pakrasi</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Rocap, G" uniqKey="Rocap G">G Rocap</name>
</author>
<author><name sortKey="Larimer, F" uniqKey="Larimer F">F Larimer</name>
</author>
<author><name sortKey="Lamerdin, J" uniqKey="Lamerdin J">J Lamerdin</name>
</author>
<author><name sortKey="Malfatti, S" uniqKey="Malfatti S">S Malfatti</name>
</author>
<author><name sortKey="Chain, P" uniqKey="Chain P">P Chain</name>
</author>
<author><name sortKey="Ahlgren, N" uniqKey="Ahlgren N">N Ahlgren</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Rokney, A" uniqKey="Rokney A">A Rokney</name>
</author>
<author><name sortKey="Kobiler, O" uniqKey="Kobiler O">O Kobiler</name>
</author>
<author><name sortKey="Amir, A" uniqKey="Amir A">A Amir</name>
</author>
<author><name sortKey="Court, D" uniqKey="Court D">D Court</name>
</author>
<author><name sortKey="Stavans, J" uniqKey="Stavans J">J Stavans</name>
</author>
<author><name sortKey="Adhya, S" uniqKey="Adhya S">S Adhya</name>
</author>
<author><name sortKey="Oppenheim, A" uniqKey="Oppenheim A">A Oppenheim</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Sancar, A" uniqKey="Sancar A">A Sancar</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Sancar, G" uniqKey="Sancar G">G Sancar</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Sargentini, N" uniqKey="Sargentini N">N Sargentini</name>
</author>
<author><name sortKey="Smith, K" uniqKey="Smith K">K Smith</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Schaaper, R" uniqKey="Schaaper R">R Schaaper</name>
</author>
<author><name sortKey="Dunn, R" uniqKey="Dunn R">R Dunn</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Singh, A" uniqKey="Singh A">A Singh</name>
</author>
<author><name sortKey="Doerks, T" uniqKey="Doerks T">T Doerks</name>
</author>
<author><name sortKey="Letunic, I" uniqKey="Letunic I">I Letunic</name>
</author>
<author><name sortKey="Raes, J" uniqKey="Raes J">J Raes</name>
</author>
<author><name sortKey="Bork, P" uniqKey="Bork P">P Bork</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Storey, J" uniqKey="Storey J">J Storey</name>
</author>
<author><name sortKey="Tibshirani, R" uniqKey="Tibshirani R">R Tibshirani</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Strehl, B" uniqKey="Strehl B">B Strehl</name>
</author>
<author><name sortKey="Holtzendorff, J" uniqKey="Holtzendorff J">J Holtzendorff</name>
</author>
<author><name sortKey="Partensky, F" uniqKey="Partensky F">F Partensky</name>
</author>
<author><name sortKey="Hess, W" uniqKey="Hess W">W Hess</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Sullivan, M" uniqKey="Sullivan M">M Sullivan</name>
</author>
<author><name sortKey="Waterbury, J" uniqKey="Waterbury J">J Waterbury</name>
</author>
<author><name sortKey="Chisholm, S" uniqKey="Chisholm S">S Chisholm</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Tedetti, M" uniqKey="Tedetti M">M Tedetti</name>
</author>
<author><name sortKey="Sempere, R" uniqKey="Sempere R">R Sempere</name>
</author>
</analytic>
</biblStruct>
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