Direct 16S rRNA-seq from bacterial communities: a PCR-independent approach to simultaneously assess microbial diversity and functional activity potential of each taxon
Identifieur interne : 000035 ( Main/Merge ); précédent : 000034; suivant : 000036Direct 16S rRNA-seq from bacterial communities: a PCR-independent approach to simultaneously assess microbial diversity and functional activity potential of each taxon
Auteurs : Riccardo Rosselli [Italie] ; Ottavia Romoli [Italie] ; Nicola Vitulo [Italie] ; Alessandro Vezzi [Italie] ; Stefano Campanaro [Italie] ; Fabio De Pascale [Italie] ; Riccardo Schiavon [Italie] ; Maurizio Tiarca [Italie] ; Fabio Poletto [Italie] ; Giuseppe Concheri [Italie] ; Giorgio Valle [Italie] ; Andrea Squartini [Italie]Source :
- Scientific Reports [ 2045-2322 ] ; 2016.
Abstract
The analysis of environmental microbial communities has largely relied on a PCR-dependent amplification of genes entailing species identity as 16S rRNA. This approach is susceptible to biases depending on the level of primer matching in different species. Moreover, possible yet-to-discover taxa whose rRNA could differ enough from known ones would not be revealed. DNA-based methods moreover do not provide information on the actual physiological relevance of each taxon within an environment and are affected by the variable number of rRNA operons in different genomes. To overcome these drawbacks we propose an approach of direct sequencing of 16S ribosomal RNA without any primer- or PCR-dependent step. The method was tested on a microbial community developing in an anammox bioreactor sampled at different time-points. A conventional PCR-based amplicon pyrosequencing was run in parallel. The community resulting from direct rRNA sequencing was highly consistent with the known biochemical processes operative in the reactor. As direct rRNA-seq is based not only on taxon abundance but also on physiological activity, no comparison between its results and those from PCR-based approaches can be applied. The novel principle is in this respect proposed not as an alternative but rather as a complementary methodology in microbial community studies.
Url:
DOI: 10.1038/srep32165
PubMed: 27577787
PubMed Central: 5006002
Links toward previous steps (curation, corpus...)
- to stream Pmc, to step Corpus: 000146
- to stream Pmc, to step Curation: 000146
- to stream Pmc, to step Checkpoint: 000033
- to stream Ncbi, to step Merge: 000753
- to stream Ncbi, to step Curation: 000753
- to stream Ncbi, to step Checkpoint: 000753
Links to Exploration step
PMC:5006002Le document en format XML
<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">Direct 16S rRNA-seq from bacterial communities: a PCR-independent approach to simultaneously assess microbial diversity and functional activity potential of each taxon</title>
<author><name sortKey="Rosselli, Riccardo" sort="Rosselli, Riccardo" uniqKey="Rosselli R" first="Riccardo" last="Rosselli">Riccardo Rosselli</name>
<affiliation wicri:level="1"><nlm:aff id="a1"><institution>Department of Biology, University of Padova</institution>
, Padova,<country>Italy</country>
</nlm:aff>
<country xml:lang="fr">Italie</country>
<wicri:regionArea># see nlm:aff country strict</wicri:regionArea>
</affiliation>
</author>
<author><name sortKey="Romoli, Ottavia" sort="Romoli, Ottavia" uniqKey="Romoli O" first="Ottavia" last="Romoli">Ottavia Romoli</name>
<affiliation wicri:level="1"><nlm:aff id="a1"><institution>Department of Biology, University of Padova</institution>
, Padova,<country>Italy</country>
</nlm:aff>
<country xml:lang="fr">Italie</country>
<wicri:regionArea># see nlm:aff country strict</wicri:regionArea>
</affiliation>
</author>
<author><name sortKey="Vitulo, Nicola" sort="Vitulo, Nicola" uniqKey="Vitulo N" first="Nicola" last="Vitulo">Nicola Vitulo</name>
<affiliation wicri:level="1"><nlm:aff id="a2"><institution>Department of Biotechnology, University of Verona</institution>
, Verona 37134,<country>Italy</country>
</nlm:aff>
<country xml:lang="fr">Italie</country>
<wicri:regionArea># see nlm:aff country strict</wicri:regionArea>
</affiliation>
</author>
<author><name sortKey="Vezzi, Alessandro" sort="Vezzi, Alessandro" uniqKey="Vezzi A" first="Alessandro" last="Vezzi">Alessandro Vezzi</name>
<affiliation wicri:level="1"><nlm:aff id="a1"><institution>Department of Biology, University of Padova</institution>
, Padova,<country>Italy</country>
</nlm:aff>
<country xml:lang="fr">Italie</country>
<wicri:regionArea># see nlm:aff country strict</wicri:regionArea>
</affiliation>
</author>
<author><name sortKey="Campanaro, Stefano" sort="Campanaro, Stefano" uniqKey="Campanaro S" first="Stefano" last="Campanaro">Stefano Campanaro</name>
<affiliation wicri:level="1"><nlm:aff id="a1"><institution>Department of Biology, University of Padova</institution>
, Padova,<country>Italy</country>
</nlm:aff>
<country xml:lang="fr">Italie</country>
<wicri:regionArea># see nlm:aff country strict</wicri:regionArea>
</affiliation>
</author>
<author><name sortKey="De Pascale, Fabio" sort="De Pascale, Fabio" uniqKey="De Pascale F" first="Fabio" last="De Pascale">Fabio De Pascale</name>
<affiliation wicri:level="1"><nlm:aff id="a1"><institution>Department of Biology, University of Padova</institution>
, Padova,<country>Italy</country>
</nlm:aff>
<country xml:lang="fr">Italie</country>
<wicri:regionArea># see nlm:aff country strict</wicri:regionArea>
</affiliation>
</author>
<author><name sortKey="Schiavon, Riccardo" sort="Schiavon, Riccardo" uniqKey="Schiavon R" first="Riccardo" last="Schiavon">Riccardo Schiavon</name>
<affiliation wicri:level="1"><nlm:aff id="a1"><institution>Department of Biology, University of Padova</institution>
, Padova,<country>Italy</country>
</nlm:aff>
<country xml:lang="fr">Italie</country>
<wicri:regionArea># see nlm:aff country strict</wicri:regionArea>
</affiliation>
</author>
<author><name sortKey="Tiarca, Maurizio" sort="Tiarca, Maurizio" uniqKey="Tiarca M" first="Maurizio" last="Tiarca">Maurizio Tiarca</name>
<affiliation wicri:level="1"><nlm:aff id="a3"><institution>RWL Water/Eurotec WTT, Water Treatment Technologies, S.r.l.</institution>
, Padova,<country>Italy</country>
</nlm:aff>
<country xml:lang="fr">Italie</country>
<wicri:regionArea># see nlm:aff country strict</wicri:regionArea>
</affiliation>
</author>
<author><name sortKey="Poletto, Fabio" sort="Poletto, Fabio" uniqKey="Poletto F" first="Fabio" last="Poletto">Fabio Poletto</name>
<affiliation wicri:level="1"><nlm:aff id="a3"><institution>RWL Water/Eurotec WTT, Water Treatment Technologies, S.r.l.</institution>
, Padova,<country>Italy</country>
</nlm:aff>
<country xml:lang="fr">Italie</country>
<wicri:regionArea># see nlm:aff country strict</wicri:regionArea>
</affiliation>
</author>
<author><name sortKey="Concheri, Giuseppe" sort="Concheri, Giuseppe" uniqKey="Concheri G" first="Giuseppe" last="Concheri">Giuseppe Concheri</name>
<affiliation wicri:level="1"><nlm:aff id="a4"><institution>Department of Agronomy Animals, Food, Natural Resources and Environment, DAFNAE, University of Padova</institution>
, Legnaro (Padova)<country>Italy</country>
</nlm:aff>
<country xml:lang="fr">Italie</country>
<wicri:regionArea># see nlm:aff country strict</wicri:regionArea>
</affiliation>
</author>
<author><name sortKey="Valle, Giorgio" sort="Valle, Giorgio" uniqKey="Valle G" first="Giorgio" last="Valle">Giorgio Valle</name>
<affiliation wicri:level="1"><nlm:aff id="a1"><institution>Department of Biology, University of Padova</institution>
, Padova,<country>Italy</country>
</nlm:aff>
<country xml:lang="fr">Italie</country>
<wicri:regionArea># see nlm:aff country strict</wicri:regionArea>
</affiliation>
</author>
<author><name sortKey="Squartini, Andrea" sort="Squartini, Andrea" uniqKey="Squartini A" first="Andrea" last="Squartini">Andrea Squartini</name>
<affiliation wicri:level="1"><nlm:aff id="a4"><institution>Department of Agronomy Animals, Food, Natural Resources and Environment, DAFNAE, University of Padova</institution>
, Legnaro (Padova)<country>Italy</country>
</nlm:aff>
<country xml:lang="fr">Italie</country>
<wicri:regionArea># see nlm:aff country strict</wicri:regionArea>
</affiliation>
</author>
</titleStmt>
<publicationStmt><idno type="wicri:source">PMC</idno>
<idno type="pmid">27577787</idno>
<idno type="pmc">5006002</idno>
<idno type="url">http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5006002</idno>
<idno type="RBID">PMC:5006002</idno>
<idno type="doi">10.1038/srep32165</idno>
<date when="2016">2016</date>
<idno type="wicri:Area/Pmc/Corpus">000146</idno>
<idno type="wicri:Area/Pmc/Curation">000146</idno>
<idno type="wicri:Area/Pmc/Checkpoint">000033</idno>
<idno type="wicri:Area/Ncbi/Merge">000753</idno>
<idno type="wicri:Area/Ncbi/Curation">000753</idno>
<idno type="wicri:Area/Ncbi/Checkpoint">000753</idno>
<idno type="wicri:Area/Main/Merge">000035</idno>
</publicationStmt>
<sourceDesc><biblStruct><analytic><title xml:lang="en" level="a" type="main">Direct 16S rRNA-seq from bacterial communities: a PCR-independent approach to simultaneously assess microbial diversity and functional activity potential of each taxon</title>
<author><name sortKey="Rosselli, Riccardo" sort="Rosselli, Riccardo" uniqKey="Rosselli R" first="Riccardo" last="Rosselli">Riccardo Rosselli</name>
<affiliation wicri:level="1"><nlm:aff id="a1"><institution>Department of Biology, University of Padova</institution>
, Padova,<country>Italy</country>
</nlm:aff>
<country xml:lang="fr">Italie</country>
<wicri:regionArea># see nlm:aff country strict</wicri:regionArea>
</affiliation>
</author>
<author><name sortKey="Romoli, Ottavia" sort="Romoli, Ottavia" uniqKey="Romoli O" first="Ottavia" last="Romoli">Ottavia Romoli</name>
<affiliation wicri:level="1"><nlm:aff id="a1"><institution>Department of Biology, University of Padova</institution>
, Padova,<country>Italy</country>
</nlm:aff>
<country xml:lang="fr">Italie</country>
<wicri:regionArea># see nlm:aff country strict</wicri:regionArea>
</affiliation>
</author>
<author><name sortKey="Vitulo, Nicola" sort="Vitulo, Nicola" uniqKey="Vitulo N" first="Nicola" last="Vitulo">Nicola Vitulo</name>
<affiliation wicri:level="1"><nlm:aff id="a2"><institution>Department of Biotechnology, University of Verona</institution>
, Verona 37134,<country>Italy</country>
</nlm:aff>
<country xml:lang="fr">Italie</country>
<wicri:regionArea># see nlm:aff country strict</wicri:regionArea>
</affiliation>
</author>
<author><name sortKey="Vezzi, Alessandro" sort="Vezzi, Alessandro" uniqKey="Vezzi A" first="Alessandro" last="Vezzi">Alessandro Vezzi</name>
<affiliation wicri:level="1"><nlm:aff id="a1"><institution>Department of Biology, University of Padova</institution>
, Padova,<country>Italy</country>
</nlm:aff>
<country xml:lang="fr">Italie</country>
<wicri:regionArea># see nlm:aff country strict</wicri:regionArea>
</affiliation>
</author>
<author><name sortKey="Campanaro, Stefano" sort="Campanaro, Stefano" uniqKey="Campanaro S" first="Stefano" last="Campanaro">Stefano Campanaro</name>
<affiliation wicri:level="1"><nlm:aff id="a1"><institution>Department of Biology, University of Padova</institution>
, Padova,<country>Italy</country>
</nlm:aff>
<country xml:lang="fr">Italie</country>
<wicri:regionArea># see nlm:aff country strict</wicri:regionArea>
</affiliation>
</author>
<author><name sortKey="De Pascale, Fabio" sort="De Pascale, Fabio" uniqKey="De Pascale F" first="Fabio" last="De Pascale">Fabio De Pascale</name>
<affiliation wicri:level="1"><nlm:aff id="a1"><institution>Department of Biology, University of Padova</institution>
, Padova,<country>Italy</country>
</nlm:aff>
<country xml:lang="fr">Italie</country>
<wicri:regionArea># see nlm:aff country strict</wicri:regionArea>
</affiliation>
</author>
<author><name sortKey="Schiavon, Riccardo" sort="Schiavon, Riccardo" uniqKey="Schiavon R" first="Riccardo" last="Schiavon">Riccardo Schiavon</name>
<affiliation wicri:level="1"><nlm:aff id="a1"><institution>Department of Biology, University of Padova</institution>
, Padova,<country>Italy</country>
</nlm:aff>
<country xml:lang="fr">Italie</country>
<wicri:regionArea># see nlm:aff country strict</wicri:regionArea>
</affiliation>
</author>
<author><name sortKey="Tiarca, Maurizio" sort="Tiarca, Maurizio" uniqKey="Tiarca M" first="Maurizio" last="Tiarca">Maurizio Tiarca</name>
<affiliation wicri:level="1"><nlm:aff id="a3"><institution>RWL Water/Eurotec WTT, Water Treatment Technologies, S.r.l.</institution>
, Padova,<country>Italy</country>
</nlm:aff>
<country xml:lang="fr">Italie</country>
<wicri:regionArea># see nlm:aff country strict</wicri:regionArea>
</affiliation>
</author>
<author><name sortKey="Poletto, Fabio" sort="Poletto, Fabio" uniqKey="Poletto F" first="Fabio" last="Poletto">Fabio Poletto</name>
<affiliation wicri:level="1"><nlm:aff id="a3"><institution>RWL Water/Eurotec WTT, Water Treatment Technologies, S.r.l.</institution>
, Padova,<country>Italy</country>
</nlm:aff>
<country xml:lang="fr">Italie</country>
<wicri:regionArea># see nlm:aff country strict</wicri:regionArea>
</affiliation>
</author>
<author><name sortKey="Concheri, Giuseppe" sort="Concheri, Giuseppe" uniqKey="Concheri G" first="Giuseppe" last="Concheri">Giuseppe Concheri</name>
<affiliation wicri:level="1"><nlm:aff id="a4"><institution>Department of Agronomy Animals, Food, Natural Resources and Environment, DAFNAE, University of Padova</institution>
, Legnaro (Padova)<country>Italy</country>
</nlm:aff>
<country xml:lang="fr">Italie</country>
<wicri:regionArea># see nlm:aff country strict</wicri:regionArea>
</affiliation>
</author>
<author><name sortKey="Valle, Giorgio" sort="Valle, Giorgio" uniqKey="Valle G" first="Giorgio" last="Valle">Giorgio Valle</name>
<affiliation wicri:level="1"><nlm:aff id="a1"><institution>Department of Biology, University of Padova</institution>
, Padova,<country>Italy</country>
</nlm:aff>
<country xml:lang="fr">Italie</country>
<wicri:regionArea># see nlm:aff country strict</wicri:regionArea>
</affiliation>
</author>
<author><name sortKey="Squartini, Andrea" sort="Squartini, Andrea" uniqKey="Squartini A" first="Andrea" last="Squartini">Andrea Squartini</name>
<affiliation wicri:level="1"><nlm:aff id="a4"><institution>Department of Agronomy Animals, Food, Natural Resources and Environment, DAFNAE, University of Padova</institution>
, Legnaro (Padova)<country>Italy</country>
</nlm:aff>
<country xml:lang="fr">Italie</country>
<wicri:regionArea># see nlm:aff country strict</wicri:regionArea>
</affiliation>
</author>
</analytic>
<series><title level="j">Scientific Reports</title>
<idno type="eISSN">2045-2322</idno>
<imprint><date when="2016">2016</date>
</imprint>
</series>
</biblStruct>
</sourceDesc>
</fileDesc>
<profileDesc><textClass></textClass>
</profileDesc>
</teiHeader>
<front><div type="abstract" xml:lang="en"><p>The analysis of environmental microbial communities has largely relied on a PCR-dependent amplification of genes entailing species identity as 16S rRNA. This approach is susceptible to biases depending on the level of primer matching in different species. Moreover, possible yet-to-discover taxa whose rRNA could differ enough from known ones would not be revealed. DNA-based methods moreover do not provide information on the actual physiological relevance of each taxon within an environment and are affected by the variable number of rRNA operons in different genomes. To overcome these drawbacks we propose an approach of direct sequencing of 16S ribosomal RNA without any primer- or PCR-dependent step. The method was tested on a microbial community developing in an anammox bioreactor sampled at different time-points. A conventional PCR-based amplicon pyrosequencing was run in parallel. The community resulting from direct rRNA sequencing was highly consistent with the known biochemical processes operative in the reactor. As direct rRNA-seq is based not only on taxon abundance but also on physiological activity, no comparison between its results and those from PCR-based approaches can be applied. The novel principle is in this respect proposed not as an alternative but rather as a complementary methodology in microbial community studies.</p>
</div>
</front>
<back><div1 type="bibliography"><listBibl><biblStruct><analytic><author><name sortKey="Woese, C" uniqKey="Woese C">C. Woese</name>
</author>
<author><name sortKey="Fox, G" uniqKey="Fox G">G. Fox</name>
</author>
<author><name sortKey="Zablen, L" uniqKey="Zablen L">L. Zablen</name>
</author>
<author><name sortKey="Uchida, T" uniqKey="Uchida T">T. Uchida</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Woese, C R" uniqKey="Woese C">C. R. Woese</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Rzhetsky, A" uniqKey="Rzhetsky A">A. Rzhetsky</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Otsuka, J" uniqKey="Otsuka J">J. Otsuka</name>
</author>
<author><name sortKey="Terai, G" uniqKey="Terai G">G. Terai</name>
</author>
<author><name sortKey="Nakano, T" uniqKey="Nakano T">T. Nakano</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Wang, H" uniqKey="Wang H">H. Wang</name>
</author>
<author><name sortKey="Hickey, D" uniqKey="Hickey D">D. Hickey</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Smit, S" uniqKey="Smit S">S. Smit</name>
</author>
<author><name sortKey="Widmann, J" uniqKey="Widmann J">J. Widmann</name>
</author>
<author><name sortKey="Knight, R" uniqKey="Knight R">R. Knight</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Van De Peer, Y" uniqKey="Van De Peer Y">Y. Van De Peer</name>
</author>
<author><name sortKey="Chapelle, S" uniqKey="Chapelle S">S. Chapelle</name>
</author>
<author><name sortKey="De Wachter, R" uniqKey="De Wachter R">R. De Wachter</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Ashelford, K E" uniqKey="Ashelford K">K. E. Ashelford</name>
</author>
<author><name sortKey="Chuzhanova, N A" uniqKey="Chuzhanova N">N. A. Chuzhanova</name>
</author>
<author><name sortKey="Fry, J C" uniqKey="Fry J">J. C. Fry</name>
</author>
<author><name sortKey="Jones, A J" uniqKey="Jones A">A. J. Jones</name>
</author>
<author><name sortKey="Weightman, A J" uniqKey="Weightman A">A. J. Weightman</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Bansal, A K" uniqKey="Bansal A">A. K. Bansal</name>
</author>
<author><name sortKey="Meyer, T E" uniqKey="Meyer T">T. E. Meyer</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Caporaso, J G" uniqKey="Caporaso J">J. G. Caporaso</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Bosshard, P P" uniqKey="Bosshard P">P. P. Bosshard</name>
</author>
<author><name sortKey="Abels, S" uniqKey="Abels S">S. Abels</name>
</author>
<author><name sortKey="Zbinden, R" uniqKey="Zbinden R">R. Zbinden</name>
</author>
<author><name sortKey="Bottger, E C" uniqKey="Bottger E">E. C. Böttger</name>
</author>
<author><name sortKey="Bo, E C" uniqKey="Bo E">E. C. Bo</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Liu, Z" uniqKey="Liu Z">Z. Liu</name>
</author>
<author><name sortKey="Lozupone, C" uniqKey="Lozupone C">C. Lozupone</name>
</author>
<author><name sortKey="Hamady, M" uniqKey="Hamady M">M. Hamady</name>
</author>
<author><name sortKey="Bushman, F D" uniqKey="Bushman F">F. D. Bushman</name>
</author>
<author><name sortKey="Knight, R" uniqKey="Knight R">R. Knight</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Bartram, A K" uniqKey="Bartram A">A. K. Bartram</name>
</author>
<author><name sortKey="Lynch, M D J" uniqKey="Lynch M">M. D. J. Lynch</name>
</author>
<author><name sortKey="Stearns, J C" uniqKey="Stearns J">J. C. Stearns</name>
</author>
<author><name sortKey="Moreno Hagelsieb, G" uniqKey="Moreno Hagelsieb G">G. Moreno-Hagelsieb</name>
</author>
<author><name sortKey="Neufeld, J D" uniqKey="Neufeld J">J. D. Neufeld</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Klindworth, A" uniqKey="Klindworth A">A. Klindworth</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Albertsen, M" uniqKey="Albertsen M">M. Albertsen</name>
</author>
<author><name sortKey="Karst, S M" uniqKey="Karst S">S. M. Karst</name>
</author>
<author><name sortKey="Ziegler, A S" uniqKey="Ziegler A">A. S. Ziegler</name>
</author>
<author><name sortKey="Kirkegaard, R H" uniqKey="Kirkegaard R">R. H. Kirkegaard</name>
</author>
<author><name sortKey="Nielsen, P H" uniqKey="Nielsen P">P. H. Nielsen</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Sun, S" uniqKey="Sun S">S. Sun</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Cole, J R" uniqKey="Cole J">J. R. Cole</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Meyer, F" uniqKey="Meyer F">F. Meyer</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Schloss, P D" uniqKey="Schloss P">P. D. Schloss</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Caporaso, J G" uniqKey="Caporaso J">J. G. Caporaso</name>
</author>
<author><name sortKey="Kuczynski, J" uniqKey="Kuczynski J">J. Kuczynski</name>
</author>
<author><name sortKey="Stombaugh, J" uniqKey="Stombaugh J">J. Stombaugh</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Miller, C S" uniqKey="Miller C">C. S. Miller</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="De Lipthay, J R" uniqKey="De Lipthay J">J. R. De Lipthay</name>
</author>
<author><name sortKey="Enzinger, C" uniqKey="Enzinger C">C. Enzinger</name>
</author>
<author><name sortKey="Johnsen, K" uniqKey="Johnsen K">K. Johnsen</name>
</author>
<author><name sortKey="Aamand, J" uniqKey="Aamand J">J. Aamand</name>
</author>
<author><name sortKey="S Rensen, S J" uniqKey="S Rensen S">S. J. Sørensen</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Thakuria, D" uniqKey="Thakuria D">D. Thakuria</name>
</author>
<author><name sortKey="Schmidt, O" uniqKey="Schmidt O">O. Schmidt</name>
</author>
<author><name sortKey="Liliensiek, A K" uniqKey="Liliensiek A">A.-K. Liliensiek</name>
</author>
<author><name sortKey="Egan, D" uniqKey="Egan D">D. Egan</name>
</author>
<author><name sortKey="Doohan, F M" uniqKey="Doohan F">F. M. Doohan</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Wommack, K E" uniqKey="Wommack K">K. E. Wommack</name>
</author>
<author><name sortKey="Bhavsar, J" uniqKey="Bhavsar J">J. Bhavsar</name>
</author>
<author><name sortKey="Ravel, J" uniqKey="Ravel J">J. Ravel</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Feinstein, L M" uniqKey="Feinstein L">L. M. Feinstein</name>
</author>
<author><name sortKey="Sul, W J" uniqKey="Sul W">W. J. Sul</name>
</author>
<author><name sortKey="Blackwood, C B" uniqKey="Blackwood C">C. B. Blackwood</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Rajendhran, J" uniqKey="Rajendhran J">J. Rajendhran</name>
</author>
<author><name sortKey="Gunasekaran, P" uniqKey="Gunasekaran P">P. Gunasekaran</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Pinto, A J" uniqKey="Pinto A">A. J. Pinto</name>
</author>
<author><name sortKey="Raskin, L" uniqKey="Raskin L">L. Raskin</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Cai, L" uniqKey="Cai L">L. Cai</name>
</author>
<author><name sortKey="Ye, L" uniqKey="Ye L">L. Ye</name>
</author>
<author><name sortKey="Tong, A H Y" uniqKey="Tong A">A. H. Y. Tong</name>
</author>
<author><name sortKey="Lok, S" uniqKey="Lok S">S. Lok</name>
</author>
<author><name sortKey="Zhang, T" uniqKey="Zhang T">T. Zhang</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Soergel, D A W" uniqKey="Soergel D">D. A. W. Soergel</name>
</author>
<author><name sortKey="Dey, N" uniqKey="Dey N">N. Dey</name>
</author>
<author><name sortKey="Knight, R" uniqKey="Knight R">R. Knight</name>
</author>
<author><name sortKey="Brenner, S E" uniqKey="Brenner S">S. E. Brenner.</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Huse, S" uniqKey="Huse S">S. Huse</name>
</author>
<author><name sortKey="Welch, D" uniqKey="Welch D">D. Welch</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Kunin, V" uniqKey="Kunin V">V. Kunin</name>
</author>
<author><name sortKey="Engelbrektson, A" uniqKey="Engelbrektson A">A. Engelbrektson</name>
</author>
<author><name sortKey="Ochman, H" uniqKey="Ochman H">H. Ochman</name>
</author>
<author><name sortKey="Hugenholtz, P" uniqKey="Hugenholtz P">P. Hugenholtz</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Rosen, G L" uniqKey="Rosen G">G. L. Rosen</name>
</author>
<author><name sortKey="Polikar, R" uniqKey="Polikar R">R. Polikar</name>
</author>
<author><name sortKey="Caseiro, D A" uniqKey="Caseiro D">D. A. Caseiro</name>
</author>
<author><name sortKey="Essinger, S D" uniqKey="Essinger S">S. D. Essinger</name>
</author>
<author><name sortKey="Sokhansanj, B A" uniqKey="Sokhansanj B">B. A. Sokhansanj</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Bowen De Le N, K" uniqKey="Bowen De Le N K">K. Bowen De León</name>
</author>
<author><name sortKey="Ramsay, B D" uniqKey="Ramsay B">B. D. Ramsay</name>
</author>
<author><name sortKey="Fields, M W" uniqKey="Fields M">M. W. Fields</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Lee, C K" uniqKey="Lee C">C. K. Lee</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Acinas, S G" uniqKey="Acinas S">S. G. Acinas</name>
</author>
<author><name sortKey="Sarma Rupavtarm, R" uniqKey="Sarma Rupavtarm R">R. Sarma-Rupavtarm</name>
</author>
<author><name sortKey="Klepac Ceraj, V" uniqKey="Klepac Ceraj V">V. Klepac-Ceraj</name>
</author>
<author><name sortKey="Polz, M F" uniqKey="Polz M">M. F. Polz</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Makino, K" uniqKey="Makino K">K. Makino</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Gonzalez Escalona, N" uniqKey="Gonzalez Escalona N">N. González-Escalona</name>
</author>
<author><name sortKey="Romero, J" uniqKey="Romero J">J. Romero</name>
</author>
<author><name sortKey="Espejo, R T" uniqKey="Espejo R">R. T. Espejo</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Vezzi, A" uniqKey="Vezzi A">A. Vezzi</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Farrelly, V" uniqKey="Farrelly V">V. Farrelly</name>
</author>
<author><name sortKey="Rainey, F A" uniqKey="Rainey F">F. A. Rainey</name>
</author>
<author><name sortKey="Stackebrandt, E" uniqKey="Stackebrandt E">E. Stackebrandt</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Kembel, S W" uniqKey="Kembel S">S. W. Kembel</name>
</author>
<author><name sortKey="Wu, M" uniqKey="Wu M">M. Wu</name>
</author>
<author><name sortKey="Eisen, J A" uniqKey="Eisen J">J. A. Eisen</name>
</author>
<author><name sortKey="Green, J L" uniqKey="Green J">J. L. Green</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="V Trovsk, T" uniqKey="V Trovsk T">T. Větrovský</name>
</author>
<author><name sortKey="Baldrian, P" uniqKey="Baldrian P">P. Baldrian</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Kamke, J" uniqKey="Kamke J">J. Kamke</name>
</author>
<author><name sortKey="Taylor, M W" uniqKey="Taylor M">M. W. Taylor</name>
</author>
<author><name sortKey="Schmitt, S" uniqKey="Schmitt S">S. Schmitt</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Wemheuer, B" uniqKey="Wemheuer B">B. Wemheuer</name>
</author>
<author><name sortKey="Wemheuer, F" uniqKey="Wemheuer F">F. Wemheuer</name>
</author>
<author><name sortKey="Daniel, R" uniqKey="Daniel R">R. Daniel</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Sharpton, T J" uniqKey="Sharpton T">T. J. Sharpton</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Poretsky, R" uniqKey="Poretsky R">R. Poretsky</name>
</author>
<author><name sortKey="Rodriguez, R L M" uniqKey="Rodriguez R">R. L. M. Rodriguez</name>
</author>
<author><name sortKey="Luo, C" uniqKey="Luo C">C. Luo</name>
</author>
<author><name sortKey="Tsementzi, D" uniqKey="Tsementzi D">D. Tsementzi</name>
</author>
<author><name sortKey="Konstantinidis, K T" uniqKey="Konstantinidis K">K. T. Konstantinidis</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Sharpton, T J" uniqKey="Sharpton T">T. J. Sharpton</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Tveit, A T" uniqKey="Tveit A">A. T. Tveit</name>
</author>
<author><name sortKey="Urich, T" uniqKey="Urich T">T. Urich</name>
</author>
<author><name sortKey="Svenning, M M" uniqKey="Svenning M">M. M. Svenning</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Ran, W" uniqKey="Ran W">W. Ran</name>
</author>
<author><name sortKey="Higgs, P G" uniqKey="Higgs P">P. G. Higgs</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Jun Jin, D" uniqKey="Jun Jin D">D. Jun Jin</name>
</author>
<author><name sortKey="Cagliero, C" uniqKey="Cagliero C">C. Cagliero</name>
</author>
<author><name sortKey="Zhou, Y N" uniqKey="Zhou Y">Y. N. Zhou</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Blazewicz, S J" uniqKey="Blazewicz S">S. J. Blazewicz</name>
</author>
<author><name sortKey="Barnard, R L" uniqKey="Barnard R">R. L. Barnard</name>
</author>
<author><name sortKey="Daly, R A" uniqKey="Daly R">R. A. Daly</name>
</author>
<author><name sortKey="Firestone, M K" uniqKey="Firestone M">M. K. Firestone</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Mitra, S" uniqKey="Mitra S">S. Mitra</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Herlemann, D P R" uniqKey="Herlemann D">D. P. R. Herlemann</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Baker, G C" uniqKey="Baker G">G. C. Baker</name>
</author>
<author><name sortKey="Smith, J J" uniqKey="Smith J">J. J. Smith</name>
</author>
<author><name sortKey="Cowan, D A" uniqKey="Cowan D">D. A. Cowan</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Sogin, M L" uniqKey="Sogin M">M. L. Sogin</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Caporaso, J G" uniqKey="Caporaso J">J. G. Caporaso</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Park, H" uniqKey="Park H">H. Park</name>
</author>
<author><name sortKey="Rosenthal, A" uniqKey="Rosenthal A">A. Rosenthal</name>
</author>
<author><name sortKey="Ramalingam, K" uniqKey="Ramalingam K">K. Ramalingam</name>
</author>
<author><name sortKey="Fillos, J" uniqKey="Fillos J">J. Fillos</name>
</author>
<author><name sortKey="Chandran, K" uniqKey="Chandran K">K. Chandran</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Tang, C" uniqKey="Tang C">C. Tang</name>
</author>
<author><name sortKey="He, R" uniqKey="He R">R. He</name>
</author>
<author><name sortKey="Zheng, P" uniqKey="Zheng P">P. Zheng</name>
</author>
<author><name sortKey="Chai, L" uniqKey="Chai L">L. Chai</name>
</author>
<author><name sortKey="Min, X" uniqKey="Min X">X. Min</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Baytshtok, V" uniqKey="Baytshtok V">V. Baytshtok</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Lee, N M" uniqKey="Lee N">N. M. Lee</name>
</author>
<author><name sortKey="Welander, T" uniqKey="Welander T">T. Welander</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Fernandez, N" uniqKey="Fernandez N">N. Fernàndez</name>
</author>
<author><name sortKey="Sierra Alvarez, R" uniqKey="Sierra Alvarez R">R. Sierra-Alvarez</name>
</author>
<author><name sortKey="Field, J A" uniqKey="Field J">J. A. Field</name>
</author>
<author><name sortKey="Amils, R" uniqKey="Amils R">R. Amils</name>
</author>
<author><name sortKey="Sanz, J L" uniqKey="Sanz J">J. L. Sanz</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Van Der Star, W R L" uniqKey="Van Der Star W">W. R. L. Van Der Star</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Neihardt, F C" uniqKey="Neihardt F">F. C. Neihardt</name>
</author>
<author><name sortKey="Umbarger, H E" uniqKey="Umbarger H">H. E. Umbarger</name>
</author>
<author><name sortKey="Neidhardt, F C" uniqKey="Neidhardt F">F. C. Neidhardt</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Gourse, R L" uniqKey="Gourse R">R. L. Gourse</name>
</author>
<author><name sortKey="Gaal, T" uniqKey="Gaal T">T. Gaal</name>
</author>
<author><name sortKey="Bartlett, M S" uniqKey="Bartlett M">M. S. Bartlett</name>
</author>
<author><name sortKey="Appleman, J A" uniqKey="Appleman J">J. A. Appleman</name>
</author>
<author><name sortKey="Ross, W" uniqKey="Ross W">W. Ross</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Rastogi, R" uniqKey="Rastogi R">R. Rastogi</name>
</author>
<author><name sortKey="Wu, M" uniqKey="Wu M">M. Wu</name>
</author>
<author><name sortKey="Dasgupta, I" uniqKey="Dasgupta I">I. Dasgupta</name>
</author>
<author><name sortKey="Fox, G E" uniqKey="Fox G">G. E. Fox</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Brown, C T" uniqKey="Brown C">C. T. Brown</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Campagna, D" uniqKey="Campagna D">D. Campagna</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Li, W" uniqKey="Li W">W. Li</name>
</author>
<author><name sortKey="Godzik, A" uniqKey="Godzik A">A. Godzik</name>
</author>
</analytic>
</biblStruct>
</listBibl>
</div1>
</back>
</TEI>
</record>
Pour manipuler ce document sous Unix (Dilib)
EXPLOR_STEP=$WICRI_ROOT/Ticri/CIDE/explor/CyberinfraV1/Data/Main/Merge
HfdSelect -h $EXPLOR_STEP/biblio.hfd -nk 000035 | SxmlIndent | more
Ou
HfdSelect -h $EXPLOR_AREA/Data/Main/Merge/biblio.hfd -nk 000035 | SxmlIndent | more
Pour mettre un lien sur cette page dans le réseau Wicri
{{Explor lien |wiki= Ticri/CIDE |area= CyberinfraV1 |flux= Main |étape= Merge |type= RBID |clé= PMC:5006002 |texte= Direct 16S rRNA-seq from bacterial communities: a PCR-independent approach to simultaneously assess microbial diversity and functional activity potential of each taxon }}
Pour générer des pages wiki
HfdIndexSelect -h $EXPLOR_AREA/Data/Main/Merge/RBID.i -Sk "pubmed:27577787" \ | HfdSelect -Kh $EXPLOR_AREA/Data/Main/Merge/biblio.hfd \ | NlmPubMed2Wicri -a CyberinfraV1
![]() | This area was generated with Dilib version V0.6.25. | ![]() |