The Shigella flexneri OspB effector: an early immunomodulator.
Identifieur interne : 000150 ( PubMed/Corpus ); précédent : 000149; suivant : 000151The Shigella flexneri OspB effector: an early immunomodulator.
Auteurs : Cecilia Ambrosi ; Monica Pompili ; Daniela Scribano ; Dolores Limongi ; Andrea Petrucca ; Sonia Cannavacciuolo ; Serena Schippa ; Carlo Zagaglia ; Milena Grossi ; Mauro NicolettiSource :
- International journal of medical microbiology : IJMM [ 1618-0607 ] ; 2015.
English descriptors
- KwdEn :
- MESH :
- chemical , metabolism : Antigens, Bacterial, Bacterial Outer Membrane Proteins, Immunologic Factors.
- chemical , secretion : Cytokines.
- immunology : Shigella flexneri.
- physiology : Shigella flexneri.
- Caco-2 Cells, HeLa Cells, Host-Pathogen Interactions, Humans, Signal Transduction.
Abstract
Through the action of the type three secretion system (T3SS) Shigella flexneri delivers several effectors into host cells to promote cellular invasion, multiplication and to exploit host-cell signaling pathways to modulate the host innate immune response. Although much progress has been made in the understanding of many type III effectors, the molecular and cellular mechanism of the OspB effector is still poorly characterized. In this study we present new evidence that better elucidates the role of OspB as pro-inflammatory factor at very early stages of infection. Indeed, we demonstrate that, during the first hour of infection, OspB is required for full activation of ERK1/2 and p38 MAPKs and the cytosolic phospholipase A(2) (cPLA(2)). Activation of cPLA(2) ultimately leads to the production and secretion of PMN chemoattractant metabolite(s) uncoupled with release of IL-8. Moreover, we also present evidence that OspB is required for the development of the full and promptly inflammatory reaction characteristic of S. flexneri wild-type infection in vivo. Based on OspB and OspF similarity (both effectors share similar transcription regulation, temporal secretion into host cells and nuclear localization) we hypothesized that OspB and OspF effectors may form a pair aimed at modulating the host cell response throughout the infection process, with opposite effects. A model is presented to illustrate how OspB activity would promote S. flexneri invasion and bacterial dissemination at early critical phases of infection.
DOI: 10.1016/j.ijmm.2014.11.004
PubMed: 25434600
Links to Exploration step
pubmed:25434600Le document en format XML
<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">The Shigella flexneri OspB effector: an early immunomodulator.</title><author><name sortKey="Ambrosi, Cecilia" sort="Ambrosi, Cecilia" uniqKey="Ambrosi C" first="Cecilia" last="Ambrosi">Cecilia Ambrosi</name><affiliation><nlm:affiliation>Dipartimento di Scienze di Sanità Pubblica e Malattie Infettive, "Sapienza" Università di Roma, 00185 Rome, Italy. Electronic address: cecilia.ambrosi@uniroma1.it.</nlm:affiliation></affiliation></author><author><name sortKey="Pompili, Monica" sort="Pompili, Monica" uniqKey="Pompili M" first="Monica" last="Pompili">Monica Pompili</name><affiliation><nlm:affiliation>Dipartimento di Scienze di Sanità Pubblica e Malattie Infettive, "Sapienza" Università di Roma, 00185 Rome, Italy.</nlm:affiliation></affiliation></author><author><name sortKey="Scribano, Daniela" sort="Scribano, Daniela" uniqKey="Scribano D" first="Daniela" last="Scribano">Daniela Scribano</name><affiliation><nlm:affiliation>Dipartimento di Scienze Sperimentali e Cliniche, Università "G. D'Annunzio", 66100 Chieti, Italy.</nlm:affiliation></affiliation></author><author><name sortKey="Limongi, Dolores" sort="Limongi, Dolores" uniqKey="Limongi D" first="Dolores" last="Limongi">Dolores Limongi</name><affiliation><nlm:affiliation>San Raffaele, Istituto di Ricovero e Cura a Carattere Scientifico IRCCS, Università Telematica San Raffaele Rome, Italy.</nlm:affiliation></affiliation></author><author><name sortKey="Petrucca, Andrea" sort="Petrucca, Andrea" uniqKey="Petrucca A" first="Andrea" last="Petrucca">Andrea Petrucca</name><affiliation><nlm:affiliation>Laboratorio di Microbiologia Clinica, II Facoltà di Medicina e Chirurgia, Ospedale "Sant'Andrea", 00189 Rome, Italy.</nlm:affiliation></affiliation></author><author><name sortKey="Cannavacciuolo, Sonia" sort="Cannavacciuolo, Sonia" uniqKey="Cannavacciuolo S" first="Sonia" last="Cannavacciuolo">Sonia Cannavacciuolo</name><affiliation><nlm:affiliation>Dipartimento di Scienze di Sanità Pubblica e Malattie Infettive, "Sapienza" Università di Roma, 00185 Rome, Italy.</nlm:affiliation></affiliation></author><author><name sortKey="Schippa, Serena" sort="Schippa, Serena" uniqKey="Schippa S" first="Serena" last="Schippa">Serena Schippa</name><affiliation><nlm:affiliation>Dipartimento di Scienze di Sanità Pubblica e Malattie Infettive, "Sapienza" Università di Roma, 00185 Rome, Italy.</nlm:affiliation></affiliation></author><author><name sortKey="Zagaglia, Carlo" sort="Zagaglia, Carlo" uniqKey="Zagaglia C" first="Carlo" last="Zagaglia">Carlo Zagaglia</name><affiliation><nlm:affiliation>Dipartimento di Scienze di Sanità Pubblica e Malattie Infettive, "Sapienza" Università di Roma, 00185 Rome, Italy.</nlm:affiliation></affiliation></author><author><name sortKey="Grossi, Milena" sort="Grossi, Milena" uniqKey="Grossi M" first="Milena" last="Grossi">Milena Grossi</name><affiliation><nlm:affiliation>Dipartimento di Biologia e Biotecnologie Charles Darwin, "Sapienza" Università di Roma, 00185 Rome, Italy.</nlm:affiliation></affiliation></author><author><name sortKey="Nicoletti, Mauro" sort="Nicoletti, Mauro" uniqKey="Nicoletti M" first="Mauro" last="Nicoletti">Mauro Nicoletti</name><affiliation><nlm:affiliation>Dipartimento di Scienze Sperimentali e Cliniche, Università "G. D'Annunzio", 66100 Chieti, Italy. Electronic address: mauro.nicoletti@uniroma1.it.</nlm:affiliation></affiliation></author></titleStmt><publicationStmt><idno type="wicri:source">PubMed</idno><date when="2015">2015</date><idno type="RBID">pubmed:25434600</idno><idno type="pmid">25434600</idno><idno type="doi">10.1016/j.ijmm.2014.11.004</idno><idno type="wicri:Area/PubMed/Corpus">000150</idno><idno type="wicri:explorRef" wicri:stream="PubMed" wicri:step="Corpus" wicri:corpus="PubMed">000150</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en">The Shigella flexneri OspB effector: an early immunomodulator.</title><author><name sortKey="Ambrosi, Cecilia" sort="Ambrosi, Cecilia" uniqKey="Ambrosi C" first="Cecilia" last="Ambrosi">Cecilia Ambrosi</name><affiliation><nlm:affiliation>Dipartimento di Scienze di Sanità Pubblica e Malattie Infettive, "Sapienza" Università di Roma, 00185 Rome, Italy. Electronic address: cecilia.ambrosi@uniroma1.it.</nlm:affiliation></affiliation></author><author><name sortKey="Pompili, Monica" sort="Pompili, Monica" uniqKey="Pompili M" first="Monica" last="Pompili">Monica Pompili</name><affiliation><nlm:affiliation>Dipartimento di Scienze di Sanità Pubblica e Malattie Infettive, "Sapienza" Università di Roma, 00185 Rome, Italy.</nlm:affiliation></affiliation></author><author><name sortKey="Scribano, Daniela" sort="Scribano, Daniela" uniqKey="Scribano D" first="Daniela" last="Scribano">Daniela Scribano</name><affiliation><nlm:affiliation>Dipartimento di Scienze Sperimentali e Cliniche, Università "G. D'Annunzio", 66100 Chieti, Italy.</nlm:affiliation></affiliation></author><author><name sortKey="Limongi, Dolores" sort="Limongi, Dolores" uniqKey="Limongi D" first="Dolores" last="Limongi">Dolores Limongi</name><affiliation><nlm:affiliation>San Raffaele, Istituto di Ricovero e Cura a Carattere Scientifico IRCCS, Università Telematica San Raffaele Rome, Italy.</nlm:affiliation></affiliation></author><author><name sortKey="Petrucca, Andrea" sort="Petrucca, Andrea" uniqKey="Petrucca A" first="Andrea" last="Petrucca">Andrea Petrucca</name><affiliation><nlm:affiliation>Laboratorio di Microbiologia Clinica, II Facoltà di Medicina e Chirurgia, Ospedale "Sant'Andrea", 00189 Rome, Italy.</nlm:affiliation></affiliation></author><author><name sortKey="Cannavacciuolo, Sonia" sort="Cannavacciuolo, Sonia" uniqKey="Cannavacciuolo S" first="Sonia" last="Cannavacciuolo">Sonia Cannavacciuolo</name><affiliation><nlm:affiliation>Dipartimento di Scienze di Sanità Pubblica e Malattie Infettive, "Sapienza" Università di Roma, 00185 Rome, Italy.</nlm:affiliation></affiliation></author><author><name sortKey="Schippa, Serena" sort="Schippa, Serena" uniqKey="Schippa S" first="Serena" last="Schippa">Serena Schippa</name><affiliation><nlm:affiliation>Dipartimento di Scienze di Sanità Pubblica e Malattie Infettive, "Sapienza" Università di Roma, 00185 Rome, Italy.</nlm:affiliation></affiliation></author><author><name sortKey="Zagaglia, Carlo" sort="Zagaglia, Carlo" uniqKey="Zagaglia C" first="Carlo" last="Zagaglia">Carlo Zagaglia</name><affiliation><nlm:affiliation>Dipartimento di Scienze di Sanità Pubblica e Malattie Infettive, "Sapienza" Università di Roma, 00185 Rome, Italy.</nlm:affiliation></affiliation></author><author><name sortKey="Grossi, Milena" sort="Grossi, Milena" uniqKey="Grossi M" first="Milena" last="Grossi">Milena Grossi</name><affiliation><nlm:affiliation>Dipartimento di Biologia e Biotecnologie Charles Darwin, "Sapienza" Università di Roma, 00185 Rome, Italy.</nlm:affiliation></affiliation></author><author><name sortKey="Nicoletti, Mauro" sort="Nicoletti, Mauro" uniqKey="Nicoletti M" first="Mauro" last="Nicoletti">Mauro Nicoletti</name><affiliation><nlm:affiliation>Dipartimento di Scienze Sperimentali e Cliniche, Università "G. D'Annunzio", 66100 Chieti, Italy. Electronic address: mauro.nicoletti@uniroma1.it.</nlm:affiliation></affiliation></author></analytic><series><title level="j">International journal of medical microbiology : IJMM</title><idno type="eISSN">1618-0607</idno><imprint><date when="2015" type="published">2015</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Antigens, Bacterial (metabolism)</term><term>Bacterial Outer Membrane Proteins (metabolism)</term><term>Caco-2 Cells</term><term>Cytokines (secretion)</term><term>HeLa Cells</term><term>Host-Pathogen Interactions</term><term>Humans</term><term>Immunologic Factors (metabolism)</term><term>Shigella flexneri (immunology)</term><term>Shigella flexneri (physiology)</term><term>Signal Transduction</term></keywords><keywords scheme="MESH" type="chemical" qualifier="metabolism" xml:lang="en"><term>Antigens, Bacterial</term><term>Bacterial Outer Membrane Proteins</term><term>Immunologic Factors</term></keywords><keywords scheme="MESH" type="chemical" qualifier="secretion" xml:lang="en"><term>Cytokines</term></keywords><keywords scheme="MESH" qualifier="immunology" xml:lang="en"><term>Shigella flexneri</term></keywords><keywords scheme="MESH" qualifier="physiology" xml:lang="en"><term>Shigella flexneri</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Caco-2 Cells</term><term>HeLa Cells</term><term>Host-Pathogen Interactions</term><term>Humans</term><term>Signal Transduction</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Through the action of the type three secretion system (T3SS) Shigella flexneri delivers several effectors into host cells to promote cellular invasion, multiplication and to exploit host-cell signaling pathways to modulate the host innate immune response. Although much progress has been made in the understanding of many type III effectors, the molecular and cellular mechanism of the OspB effector is still poorly characterized. In this study we present new evidence that better elucidates the role of OspB as pro-inflammatory factor at very early stages of infection. Indeed, we demonstrate that, during the first hour of infection, OspB is required for full activation of ERK1/2 and p38 MAPKs and the cytosolic phospholipase A(2) (cPLA(2)). Activation of cPLA(2) ultimately leads to the production and secretion of PMN chemoattractant metabolite(s) uncoupled with release of IL-8. Moreover, we also present evidence that OspB is required for the development of the full and promptly inflammatory reaction characteristic of S. flexneri wild-type infection in vivo. Based on OspB and OspF similarity (both effectors share similar transcription regulation, temporal secretion into host cells and nuclear localization) we hypothesized that OspB and OspF effectors may form a pair aimed at modulating the host cell response throughout the infection process, with opposite effects. A model is presented to illustrate how OspB activity would promote S. flexneri invasion and bacterial dissemination at early critical phases of infection.</div></front></TEI><pubmed><MedlineCitation Owner="NLM" Status="MEDLINE"><PMID Version="1">25434600</PMID><DateCreated><Year>2015</Year><Month>01</Month><Day>12</Day></DateCreated><DateCompleted><Year>2015</Year><Month>09</Month><Day>08</Day></DateCompleted><Article PubModel="Print-Electronic"><Journal><ISSN IssnType="Electronic">1618-0607</ISSN><JournalIssue CitedMedium="Internet"><Volume>305</Volume><Issue>1</Issue><PubDate><Year>2015</Year><Month>Jan</Month></PubDate></JournalIssue><Title>International journal of medical microbiology : IJMM</Title><ISOAbbreviation>Int. J. Med. Microbiol.</ISOAbbreviation></Journal><ArticleTitle>The Shigella flexneri OspB effector: an early immunomodulator.</ArticleTitle><Pagination><MedlinePgn>75-84</MedlinePgn></Pagination><ELocationID EIdType="doi" ValidYN="Y">10.1016/j.ijmm.2014.11.004</ELocationID><ELocationID EIdType="pii" ValidYN="Y">S1438-4221(14)00152-0</ELocationID><Abstract><AbstractText>Through the action of the type three secretion system (T3SS) Shigella flexneri delivers several effectors into host cells to promote cellular invasion, multiplication and to exploit host-cell signaling pathways to modulate the host innate immune response. Although much progress has been made in the understanding of many type III effectors, the molecular and cellular mechanism of the OspB effector is still poorly characterized. In this study we present new evidence that better elucidates the role of OspB as pro-inflammatory factor at very early stages of infection. Indeed, we demonstrate that, during the first hour of infection, OspB is required for full activation of ERK1/2 and p38 MAPKs and the cytosolic phospholipase A(2) (cPLA(2)). Activation of cPLA(2) ultimately leads to the production and secretion of PMN chemoattractant metabolite(s) uncoupled with release of IL-8. Moreover, we also present evidence that OspB is required for the development of the full and promptly inflammatory reaction characteristic of S. flexneri wild-type infection in vivo. Based on OspB and OspF similarity (both effectors share similar transcription regulation, temporal secretion into host cells and nuclear localization) we hypothesized that OspB and OspF effectors may form a pair aimed at modulating the host cell response throughout the infection process, with opposite effects. A model is presented to illustrate how OspB activity would promote S. flexneri invasion and bacterial dissemination at early critical phases of infection.</AbstractText><CopyrightInformation>Copyright © 2014 Elsevier GmbH. All rights reserved.</CopyrightInformation></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Ambrosi</LastName><ForeName>Cecilia</ForeName><Initials>C</Initials><AffiliationInfo><Affiliation>Dipartimento di Scienze di Sanità Pubblica e Malattie Infettive, "Sapienza" Università di Roma, 00185 Rome, Italy. Electronic address: cecilia.ambrosi@uniroma1.it.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Pompili</LastName><ForeName>Monica</ForeName><Initials>M</Initials><AffiliationInfo><Affiliation>Dipartimento di Scienze di Sanità Pubblica e Malattie Infettive, "Sapienza" Università di Roma, 00185 Rome, Italy.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Scribano</LastName><ForeName>Daniela</ForeName><Initials>D</Initials><AffiliationInfo><Affiliation>Dipartimento di Scienze Sperimentali e Cliniche, Università "G. D'Annunzio", 66100 Chieti, Italy.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Limongi</LastName><ForeName>Dolores</ForeName><Initials>D</Initials><AffiliationInfo><Affiliation>San Raffaele, Istituto di Ricovero e Cura a Carattere Scientifico IRCCS, Università Telematica San Raffaele Rome, Italy.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Petrucca</LastName><ForeName>Andrea</ForeName><Initials>A</Initials><AffiliationInfo><Affiliation>Laboratorio di Microbiologia Clinica, II Facoltà di Medicina e Chirurgia, Ospedale "Sant'Andrea", 00189 Rome, Italy.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Cannavacciuolo</LastName><ForeName>Sonia</ForeName><Initials>S</Initials><AffiliationInfo><Affiliation>Dipartimento di Scienze di Sanità Pubblica e Malattie Infettive, "Sapienza" Università di Roma, 00185 Rome, Italy.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Schippa</LastName><ForeName>Serena</ForeName><Initials>S</Initials><AffiliationInfo><Affiliation>Dipartimento di Scienze di Sanità Pubblica e Malattie Infettive, "Sapienza" Università di Roma, 00185 Rome, Italy.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Zagaglia</LastName><ForeName>Carlo</ForeName><Initials>C</Initials><AffiliationInfo><Affiliation>Dipartimento di Scienze di Sanità Pubblica e Malattie Infettive, "Sapienza" Università di Roma, 00185 Rome, Italy.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Grossi</LastName><ForeName>Milena</ForeName><Initials>M</Initials><AffiliationInfo><Affiliation>Dipartimento di Biologia e Biotecnologie Charles Darwin, "Sapienza" Università di Roma, 00185 Rome, Italy.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Nicoletti</LastName><ForeName>Mauro</ForeName><Initials>M</Initials><AffiliationInfo><Affiliation>Dipartimento di Scienze Sperimentali e Cliniche, Università "G. D'Annunzio", 66100 Chieti, Italy. Electronic address: mauro.nicoletti@uniroma1.it.</Affiliation></AffiliationInfo></Author></AuthorList><Language>eng</Language><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType><PublicationType UI="D013485">Research Support, Non-U.S. Gov't</PublicationType></PublicationTypeList><ArticleDate DateType="Electronic"><Year>2014</Year><Month>11</Month><Day>11</Day></ArticleDate></Article><MedlineJournalInfo><Country>Germany</Country><MedlineTA>Int J Med Microbiol</MedlineTA><NlmUniqueID>100898849</NlmUniqueID><ISSNLinking>1438-4221</ISSNLinking></MedlineJournalInfo><ChemicalList><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D000942">Antigens, Bacterial</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D001425">Bacterial Outer Membrane Proteins</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D016207">Cytokines</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D007155">Immunologic Factors</NameOfSubstance></Chemical></ChemicalList><CitationSubset>IM</CitationSubset><MeshHeadingList><MeshHeading><DescriptorName MajorTopicYN="N" UI="D000942">Antigens, Bacterial</DescriptorName><QualifierName MajorTopicYN="Y" UI="Q000378">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName MajorTopicYN="N" UI="D001425">Bacterial Outer Membrane Proteins</DescriptorName><QualifierName MajorTopicYN="Y" UI="Q000378">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName MajorTopicYN="N" UI="D018938">Caco-2 Cells</DescriptorName></MeshHeading><MeshHeading><DescriptorName MajorTopicYN="N" UI="D016207">Cytokines</DescriptorName><QualifierName MajorTopicYN="Y" UI="Q000557">secretion</QualifierName></MeshHeading><MeshHeading><DescriptorName MajorTopicYN="N" UI="D006367">HeLa Cells</DescriptorName></MeshHeading><MeshHeading><DescriptorName MajorTopicYN="Y" UI="D054884">Host-Pathogen Interactions</DescriptorName></MeshHeading><MeshHeading><DescriptorName MajorTopicYN="N" UI="D006801">Humans</DescriptorName></MeshHeading><MeshHeading><DescriptorName MajorTopicYN="N" UI="D007155">Immunologic Factors</DescriptorName><QualifierName MajorTopicYN="Y" UI="Q000378">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName MajorTopicYN="N" UI="D012763">Shigella flexneri</DescriptorName><QualifierName MajorTopicYN="Y" UI="Q000276">immunology</QualifierName><QualifierName MajorTopicYN="Y" UI="Q000502">physiology</QualifierName></MeshHeading><MeshHeading><DescriptorName MajorTopicYN="N" UI="D015398">Signal Transduction</DescriptorName></MeshHeading></MeshHeadingList><KeywordList Owner="NOTNLM"><Keyword MajorTopicYN="N">IL-8</Keyword><Keyword MajorTopicYN="N">MAP kinases</Keyword><Keyword MajorTopicYN="N">PMN migration</Keyword><Keyword MajorTopicYN="N">Shigella flexneri</Keyword><Keyword MajorTopicYN="N">ospB</Keyword></KeywordList></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="received"><Year>2014</Year><Month>7</Month><Day>15</Day></PubMedPubDate><PubMedPubDate PubStatus="revised"><Year>2014</Year><Month>10</Month><Day>30</Day></PubMedPubDate><PubMedPubDate PubStatus="accepted"><Year>2014</Year><Month>11</Month><Day>4</Day></PubMedPubDate><PubMedPubDate PubStatus="aheadofprint"><Year>2014</Year><Month>11</Month><Day>11</Day></PubMedPubDate><PubMedPubDate PubStatus="entrez"><Year>2014</Year><Month>12</Month><Day>2</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="pubmed"><Year>2014</Year><Month>12</Month><Day>2</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="medline"><Year>2015</Year><Month>9</Month><Day>9</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate></History><PublicationStatus>ppublish</PublicationStatus><ArticleIdList><ArticleId IdType="pubmed">25434600</ArticleId><ArticleId IdType="pii">S1438-4221(14)00152-0</ArticleId><ArticleId IdType="doi">10.1016/j.ijmm.2014.11.004</ArticleId></ArticleIdList></PubmedData></pubmed></record>Pour manipuler ce document sous Unix (Dilib)
EXPLOR_STEP=$WICRI_ROOT/Ticri/CIDE/explor/TelematiV1/Data/PubMed/Corpus
HfdSelect -h $EXPLOR_STEP/biblio.hfd -nk 000150 | SxmlIndent | more
Ou
HfdSelect -h $EXPLOR_AREA/Data/PubMed/Corpus/biblio.hfd -nk 000150 | SxmlIndent | more
Pour mettre un lien sur cette page dans le réseau Wicri
{{Explor lien
|wiki= Ticri/CIDE
|area= TelematiV1
|flux= PubMed
|étape= Corpus
|type= RBID
|clé= pubmed:25434600
|texte= The Shigella flexneri OspB effector: an early immunomodulator.
}}
Pour générer des pages wiki
HfdIndexSelect -h $EXPLOR_AREA/Data/PubMed/Corpus/RBID.i -Sk "pubmed:25434600" \
| HfdSelect -Kh $EXPLOR_AREA/Data/PubMed/Corpus/biblio.hfd \
| NlmPubMed2Wicri -a TelematiV1
| This area was generated with Dilib version V0.6.31. |  |