Gene molecular analysis and Adiponectin expression in professional Water Polo players.
Identifieur interne : 000024 ( PubMed/Corpus ); précédent : 000023; suivant : 000025Gene molecular analysis and Adiponectin expression in professional Water Polo players.
Auteurs : Ersilia Nigro ; Dino Sangiorgio ; Olga Scudiero ; Maria Ludovica Monaco ; Rita Polito ; Giovanni Villone ; Aurora DanieleSource :
- Cytokine [ 1096-0023 ] ; 2016.
Abstract
Metabolic Syndrome prevalence has reaching epidemic proportions worldwide. Adiponectin (Acrp30), and in particular its High Molecular Weight (HMW) oligomers, contributes to enhance insulin sensitivity and to reduce inflammation levels. Physical exercise improves body's biochemical balance and metabolism resulting effective in prevention of metabolic diseases. Whether improvement of metabolic features mediated by physical exercise is associated with changes in Acrp30 serum composition is not yet clarified. In the present study, we investigated total Acrp30 expression, its oligomeric status and genetic variants in adiponectin gene (ACDC) in twenty-two professional Water Polo (WP) Players and 40 age- and sex-matched controls. Anthropometric, metabolic parameters and total Acrp30 were assessed; Acrp30 oligomeric profile was characterized by Western blot as well as by FPLC analysis. ACDC gene was analyzed by direct-sequencing analysis. Significant elevated body mass index, aspartate aminotransferase and lactate dehydrogenase levels and, conversely, significantly lower concentrations of total and cholesterol low density lipoprotein were present in WP players. No significant difference was found in total Acrp30 and/or HMW oligomers. Interestingly, in WP players, a direct relationship between total Acrp30 and monocytes as well as an inverse relationship between total Acrp30 and AST levels were found. ACDC screening revealed previously described SNPs. In conclusion, our study confirms the long-term beneficial effects of high physical training on metabolism and suggests that they are not associated with Acrp30 and/or HMW oligomers changes. Moreover, the correlation of Acrp30 with monocytes in WP athletes could represent a mechanism by which Acrp30 participates in exercise-induced anti-inflammatory functions and/or cardiovascular health.
DOI: 10.1016/j.cyto.2016.03.002
PubMed: 26970705
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Salvatore 486, 80145 Napoli, Italy; Dipartimento di Medicina Molecolare e Biotecnologie Mediche, Università di Napoli Federico II, Via S. Pansini 5, 80131 Napoli, Italy.</nlm:affiliation></affiliation></author><author><name sortKey="Monaco, Maria Ludovica" sort="Monaco, Maria Ludovica" uniqKey="Monaco M" first="Maria Ludovica" last="Monaco">Maria Ludovica Monaco</name><affiliation><nlm:affiliation>CEINGE-Biotecnologie Avanzate Scarl, Via G. Salvatore 486, 80145 Napoli, Italy.</nlm:affiliation></affiliation></author><author><name sortKey="Polito, Rita" sort="Polito, Rita" uniqKey="Polito R" first="Rita" last="Polito">Rita Polito</name><affiliation><nlm:affiliation>CEINGE-Biotecnologie Avanzate Scarl, Via G. Salvatore 486, 80145 Napoli, Italy; Dipartimento di Scienze e Tecnologie Ambientali Biologiche Farmaceutiche, Seconda Università degli Studi di Napoli, Via G. Vivaldi 42, 81100 Caserta, Italy.</nlm:affiliation></affiliation></author><author><name sortKey="Villone, Giovanni" sort="Villone, Giovanni" uniqKey="Villone G" first="Giovanni" last="Villone">Giovanni Villone</name><affiliation><nlm:affiliation>Dipartimento di Medicina e di Scienze della Salute "Vincenzo Tiberio" Università degli Studi del Molise, Campobasso, Italy.</nlm:affiliation></affiliation></author><author><name sortKey="Daniele, Aurora" sort="Daniele, Aurora" uniqKey="Daniele A" first="Aurora" last="Daniele">Aurora Daniele</name><affiliation><nlm:affiliation>Dipartimento di Scienze e Tecnologie Ambientali Biologiche Farmaceutiche, Seconda Università degli Studi di Napoli, Via G. Vivaldi 42, 81100 Caserta, Italy. 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Salvatore 486, 80145 Napoli, Italy.</nlm:affiliation></affiliation></author><author><name sortKey="Sangiorgio, Dino" sort="Sangiorgio, Dino" uniqKey="Sangiorgio D" first="Dino" last="Sangiorgio">Dino Sangiorgio</name><affiliation><nlm:affiliation>Università Telematica Pegaso, Naples, Italy.</nlm:affiliation></affiliation></author><author><name sortKey="Scudiero, Olga" sort="Scudiero, Olga" uniqKey="Scudiero O" first="Olga" last="Scudiero">Olga Scudiero</name><affiliation><nlm:affiliation>CEINGE-Biotecnologie Avanzate Scarl, Via G. Salvatore 486, 80145 Napoli, Italy; Dipartimento di Medicina Molecolare e Biotecnologie Mediche, Università di Napoli Federico II, Via S. Pansini 5, 80131 Napoli, Italy.</nlm:affiliation></affiliation></author><author><name sortKey="Monaco, Maria Ludovica" sort="Monaco, Maria Ludovica" uniqKey="Monaco M" first="Maria Ludovica" last="Monaco">Maria Ludovica Monaco</name><affiliation><nlm:affiliation>CEINGE-Biotecnologie Avanzate Scarl, Via G. Salvatore 486, 80145 Napoli, Italy.</nlm:affiliation></affiliation></author><author><name sortKey="Polito, Rita" sort="Polito, Rita" uniqKey="Polito R" first="Rita" last="Polito">Rita Polito</name><affiliation><nlm:affiliation>CEINGE-Biotecnologie Avanzate Scarl, Via G. Salvatore 486, 80145 Napoli, Italy; Dipartimento di Scienze e Tecnologie Ambientali Biologiche Farmaceutiche, Seconda Università degli Studi di Napoli, Via G. Vivaldi 42, 81100 Caserta, Italy.</nlm:affiliation></affiliation></author><author><name sortKey="Villone, Giovanni" sort="Villone, Giovanni" uniqKey="Villone G" first="Giovanni" last="Villone">Giovanni Villone</name><affiliation><nlm:affiliation>Dipartimento di Medicina e di Scienze della Salute "Vincenzo Tiberio" Università degli Studi del Molise, Campobasso, Italy.</nlm:affiliation></affiliation></author><author><name sortKey="Daniele, Aurora" sort="Daniele, Aurora" uniqKey="Daniele A" first="Aurora" last="Daniele">Aurora Daniele</name><affiliation><nlm:affiliation>Dipartimento di Scienze e Tecnologie Ambientali Biologiche Farmaceutiche, Seconda Università degli Studi di Napoli, Via G. Vivaldi 42, 81100 Caserta, Italy. Electronic address: aurora.daniele@unina2.it.</nlm:affiliation></affiliation></author></analytic><series><title level="j">Cytokine</title><idno type="eISSN">1096-0023</idno><imprint><date when="2016" type="published">2016</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Metabolic Syndrome prevalence has reaching epidemic proportions worldwide. Adiponectin (Acrp30), and in particular its High Molecular Weight (HMW) oligomers, contributes to enhance insulin sensitivity and to reduce inflammation levels. Physical exercise improves body's biochemical balance and metabolism resulting effective in prevention of metabolic diseases. Whether improvement of metabolic features mediated by physical exercise is associated with changes in Acrp30 serum composition is not yet clarified. In the present study, we investigated total Acrp30 expression, its oligomeric status and genetic variants in adiponectin gene (ACDC) in twenty-two professional Water Polo (WP) Players and 40 age- and sex-matched controls. Anthropometric, metabolic parameters and total Acrp30 were assessed; Acrp30 oligomeric profile was characterized by Western blot as well as by FPLC analysis. ACDC gene was analyzed by direct-sequencing analysis. Significant elevated body mass index, aspartate aminotransferase and lactate dehydrogenase levels and, conversely, significantly lower concentrations of total and cholesterol low density lipoprotein were present in WP players. No significant difference was found in total Acrp30 and/or HMW oligomers. Interestingly, in WP players, a direct relationship between total Acrp30 and monocytes as well as an inverse relationship between total Acrp30 and AST levels were found. ACDC screening revealed previously described SNPs. In conclusion, our study confirms the long-term beneficial effects of high physical training on metabolism and suggests that they are not associated with Acrp30 and/or HMW oligomers changes. Moreover, the correlation of Acrp30 with monocytes in WP athletes could represent a mechanism by which Acrp30 participates in exercise-induced anti-inflammatory functions and/or cardiovascular health.</div></front></TEI><pubmed><MedlineCitation Owner="NLM" Status="In-Data-Review"><PMID Version="1">26970705</PMID><DateCreated><Year>2016</Year><Month>03</Month><Day>23</Day></DateCreated><Article PubModel="Print-Electronic"><Journal><ISSN IssnType="Electronic">1096-0023</ISSN><JournalIssue CitedMedium="Internet"><Volume>81</Volume><PubDate><Year>2016</Year><Month>May</Month></PubDate></JournalIssue><Title>Cytokine</Title><ISOAbbreviation>Cytokine</ISOAbbreviation></Journal><ArticleTitle>Gene molecular analysis and Adiponectin expression in professional Water Polo players.</ArticleTitle><Pagination><MedlinePgn>88-93</MedlinePgn></Pagination><ELocationID EIdType="doi" ValidYN="Y">10.1016/j.cyto.2016.03.002</ELocationID><ELocationID EIdType="pii" ValidYN="Y">S1043-4666(16)30041-2</ELocationID><Abstract><AbstractText>Metabolic Syndrome prevalence has reaching epidemic proportions worldwide. Adiponectin (Acrp30), and in particular its High Molecular Weight (HMW) oligomers, contributes to enhance insulin sensitivity and to reduce inflammation levels. Physical exercise improves body's biochemical balance and metabolism resulting effective in prevention of metabolic diseases. Whether improvement of metabolic features mediated by physical exercise is associated with changes in Acrp30 serum composition is not yet clarified. In the present study, we investigated total Acrp30 expression, its oligomeric status and genetic variants in adiponectin gene (ACDC) in twenty-two professional Water Polo (WP) Players and 40 age- and sex-matched controls. Anthropometric, metabolic parameters and total Acrp30 were assessed; Acrp30 oligomeric profile was characterized by Western blot as well as by FPLC analysis. ACDC gene was analyzed by direct-sequencing analysis. Significant elevated body mass index, aspartate aminotransferase and lactate dehydrogenase levels and, conversely, significantly lower concentrations of total and cholesterol low density lipoprotein were present in WP players. No significant difference was found in total Acrp30 and/or HMW oligomers. Interestingly, in WP players, a direct relationship between total Acrp30 and monocytes as well as an inverse relationship between total Acrp30 and AST levels were found. ACDC screening revealed previously described SNPs. In conclusion, our study confirms the long-term beneficial effects of high physical training on metabolism and suggests that they are not associated with Acrp30 and/or HMW oligomers changes. Moreover, the correlation of Acrp30 with monocytes in WP athletes could represent a mechanism by which Acrp30 participates in exercise-induced anti-inflammatory functions and/or cardiovascular health.</AbstractText><CopyrightInformation>Copyright © 2016 Elsevier Ltd. All rights reserved.</CopyrightInformation></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Nigro</LastName><ForeName>Ersilia</ForeName><Initials>E</Initials><AffiliationInfo><Affiliation>CEINGE-Biotecnologie Avanzate Scarl, Via G. Salvatore 486, 80145 Napoli, Italy.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Sangiorgio</LastName><ForeName>Dino</ForeName><Initials>D</Initials><AffiliationInfo><Affiliation>Università Telematica Pegaso, Naples, Italy.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Scudiero</LastName><ForeName>Olga</ForeName><Initials>O</Initials><AffiliationInfo><Affiliation>CEINGE-Biotecnologie Avanzate Scarl, Via G. Salvatore 486, 80145 Napoli, Italy; Dipartimento di Medicina Molecolare e Biotecnologie Mediche, Università di Napoli Federico II, Via S. Pansini 5, 80131 Napoli, Italy.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Monaco</LastName><ForeName>Maria Ludovica</ForeName><Initials>ML</Initials><AffiliationInfo><Affiliation>CEINGE-Biotecnologie Avanzate Scarl, Via G. Salvatore 486, 80145 Napoli, Italy.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Polito</LastName><ForeName>Rita</ForeName><Initials>R</Initials><AffiliationInfo><Affiliation>CEINGE-Biotecnologie Avanzate Scarl, Via G. Salvatore 486, 80145 Napoli, Italy; Dipartimento di Scienze e Tecnologie Ambientali Biologiche Farmaceutiche, Seconda Università degli Studi di Napoli, Via G. Vivaldi 42, 81100 Caserta, Italy.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Villone</LastName><ForeName>Giovanni</ForeName><Initials>G</Initials><AffiliationInfo><Affiliation>Dipartimento di Medicina e di Scienze della Salute "Vincenzo Tiberio" Università degli Studi del Molise, Campobasso, Italy.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Daniele</LastName><ForeName>Aurora</ForeName><Initials>A</Initials><AffiliationInfo><Affiliation>Dipartimento di Scienze e Tecnologie Ambientali Biologiche Farmaceutiche, Seconda Università degli Studi di Napoli, Via G. Vivaldi 42, 81100 Caserta, Italy. Electronic address: aurora.daniele@unina2.it.</Affiliation></AffiliationInfo></Author></AuthorList><Language>eng</Language><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType></PublicationTypeList><ArticleDate DateType="Electronic"><Year>2016</Year><Month>03</Month><Day>10</Day></ArticleDate></Article><MedlineJournalInfo><Country>England</Country><MedlineTA>Cytokine</MedlineTA><NlmUniqueID>9005353</NlmUniqueID><ISSNLinking>1043-4666</ISSNLinking></MedlineJournalInfo><CitationSubset>IM</CitationSubset><KeywordList Owner="NOTNLM"><Keyword MajorTopicYN="N">Adiponectin</Keyword><Keyword MajorTopicYN="N">Adiponectin polymorphisms</Keyword><Keyword MajorTopicYN="N">HMW oligomers</Keyword><Keyword MajorTopicYN="N">Water Polo players</Keyword><Keyword MajorTopicYN="N">lipid metabolism</Keyword><Keyword MajorTopicYN="N">physical exercise</Keyword></KeywordList></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="received"><Year>2016</Year><Month>2</Month><Day>4</Day></PubMedPubDate><PubMedPubDate PubStatus="revised"><Year>2016</Year><Month>3</Month><Day>3</Day></PubMedPubDate><PubMedPubDate PubStatus="accepted"><Year>2016</Year><Month>3</Month><Day>4</Day></PubMedPubDate><PubMedPubDate PubStatus="aheadofprint"><Year>2016</Year><Month>3</Month><Day>10</Day></PubMedPubDate><PubMedPubDate PubStatus="entrez"><Year>2016</Year><Month>3</Month><Day>14</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="pubmed"><Year>2016</Year><Month>3</Month><Day>14</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="medline"><Year>2016</Year><Month>3</Month><Day>14</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate></History><PublicationStatus>ppublish</PublicationStatus><ArticleIdList><ArticleId IdType="pubmed">26970705</ArticleId><ArticleId IdType="pii">S1043-4666(16)30041-2</ArticleId><ArticleId IdType="doi">10.1016/j.cyto.2016.03.002</ArticleId></ArticleIdList></PubmedData></pubmed></record>Pour manipuler ce document sous Unix (Dilib)
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