Hydrophilic Polyester Microspheres: Effect of Molecular Weight and Copolymer Composition on Release of BSA
Identifieur interne : 000174 ( Pmc/Curation ); précédent : 000173; suivant : 000175Hydrophilic Polyester Microspheres: Effect of Molecular Weight and Copolymer Composition on Release of BSA
Auteurs : Amir H. Ghassemi [Pays-Bas] ; Mies J. Van Steenbergen [Pays-Bas] ; Herre Talsma [Pays-Bas] ; Cornelus F. Van Nostrum [Pays-Bas] ; Daan J. A. Crommelin [Pays-Bas] ; Wim E. Hennink [Pays-Bas]Source :
- Pharmaceutical Research [ 0724-8741 ] ; 2010.
Abstract
To study the release of a model protein, bovine serum albumin (BSA), from microspheres of an hydroxylated aliphatic polyester, poly(lactic-co-hydroxymethyl glycolic acid) (PLHMGA).
BSA-loaded microspheres were prepared by a double emulsion solvent evaporation method. The effect of copolymer composition and the molecular weight of the copolymer on
Microspheres prepared from PLHMGA with 50% hydroxymethyl glycolic acid (HMG) showed a burst release followed by a sustained release in 5–10 days. PLHMGA microspheres prepared from a copolymer with 35% and 25% HMG showed a sustained release of BSA up to 80% for 30 and 60 days, respectively. The release of BSA was hardly affected by the molecular weight of the polymer. Fluorescence spectroscopy and SEC showed that the released BSA preserved its structural integrity. Microspheres were fully degradable, and the degradation time increased from ~20 days to 60 days when the HMG content decreased from 50% to 25%.
Taking the degradation and release data together, it can be concluded that the release of BSA from PLHMGA microspheres is governed by degradation of the microspheres.
Url:
DOI: 10.1007/s11095-010-0205-8
PubMed: 20602152
PubMed Central: 2916118
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<affiliation wicri:level="1"><nlm:aff id="Aff1">Department of Pharmaceutics, Utrecht Institute for Pharmaceutical Sciences (UIPS), Utrecht University, P.O. Box 80082, Sorbonnelaan 16, 3508 TB Utrecht, The Netherlands</nlm:aff>
<country xml:lang="fr">Pays-Bas</country>
<wicri:regionArea>Department of Pharmaceutics, Utrecht Institute for Pharmaceutical Sciences (UIPS), Utrecht University, P.O. Box 80082, Sorbonnelaan 16, 3508 TB Utrecht</wicri:regionArea>
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<author><name sortKey="Van Steenbergen, Mies J" sort="Van Steenbergen, Mies J" uniqKey="Van Steenbergen M" first="Mies J." last="Van Steenbergen">Mies J. Van Steenbergen</name>
<affiliation wicri:level="1"><nlm:aff id="Aff1">Department of Pharmaceutics, Utrecht Institute for Pharmaceutical Sciences (UIPS), Utrecht University, P.O. Box 80082, Sorbonnelaan 16, 3508 TB Utrecht, The Netherlands</nlm:aff>
<country xml:lang="fr">Pays-Bas</country>
<wicri:regionArea>Department of Pharmaceutics, Utrecht Institute for Pharmaceutical Sciences (UIPS), Utrecht University, P.O. Box 80082, Sorbonnelaan 16, 3508 TB Utrecht</wicri:regionArea>
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<author><name sortKey="Talsma, Herre" sort="Talsma, Herre" uniqKey="Talsma H" first="Herre" last="Talsma">Herre Talsma</name>
<affiliation wicri:level="1"><nlm:aff id="Aff1">Department of Pharmaceutics, Utrecht Institute for Pharmaceutical Sciences (UIPS), Utrecht University, P.O. Box 80082, Sorbonnelaan 16, 3508 TB Utrecht, The Netherlands</nlm:aff>
<country xml:lang="fr">Pays-Bas</country>
<wicri:regionArea>Department of Pharmaceutics, Utrecht Institute for Pharmaceutical Sciences (UIPS), Utrecht University, P.O. Box 80082, Sorbonnelaan 16, 3508 TB Utrecht</wicri:regionArea>
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<author><name sortKey="Van Nostrum, Cornelus F" sort="Van Nostrum, Cornelus F" uniqKey="Van Nostrum C" first="Cornelus F." last="Van Nostrum">Cornelus F. Van Nostrum</name>
<affiliation wicri:level="1"><nlm:aff id="Aff1">Department of Pharmaceutics, Utrecht Institute for Pharmaceutical Sciences (UIPS), Utrecht University, P.O. Box 80082, Sorbonnelaan 16, 3508 TB Utrecht, The Netherlands</nlm:aff>
<country xml:lang="fr">Pays-Bas</country>
<wicri:regionArea>Department of Pharmaceutics, Utrecht Institute for Pharmaceutical Sciences (UIPS), Utrecht University, P.O. Box 80082, Sorbonnelaan 16, 3508 TB Utrecht</wicri:regionArea>
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<author><name sortKey="Crommelin, Daan J A" sort="Crommelin, Daan J A" uniqKey="Crommelin D" first="Daan J. A." last="Crommelin">Daan J. A. Crommelin</name>
<affiliation wicri:level="1"><nlm:aff id="Aff1">Department of Pharmaceutics, Utrecht Institute for Pharmaceutical Sciences (UIPS), Utrecht University, P.O. Box 80082, Sorbonnelaan 16, 3508 TB Utrecht, The Netherlands</nlm:aff>
<country xml:lang="fr">Pays-Bas</country>
<wicri:regionArea>Department of Pharmaceutics, Utrecht Institute for Pharmaceutical Sciences (UIPS), Utrecht University, P.O. Box 80082, Sorbonnelaan 16, 3508 TB Utrecht</wicri:regionArea>
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<author><name sortKey="Hennink, Wim E" sort="Hennink, Wim E" uniqKey="Hennink W" first="Wim E." last="Hennink">Wim E. Hennink</name>
<affiliation wicri:level="1"><nlm:aff id="Aff1">Department of Pharmaceutics, Utrecht Institute for Pharmaceutical Sciences (UIPS), Utrecht University, P.O. Box 80082, Sorbonnelaan 16, 3508 TB Utrecht, The Netherlands</nlm:aff>
<country xml:lang="fr">Pays-Bas</country>
<wicri:regionArea>Department of Pharmaceutics, Utrecht Institute for Pharmaceutical Sciences (UIPS), Utrecht University, P.O. Box 80082, Sorbonnelaan 16, 3508 TB Utrecht</wicri:regionArea>
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<sourceDesc><biblStruct><analytic><title xml:lang="en" level="a" type="main">Hydrophilic Polyester Microspheres: Effect of Molecular Weight and Copolymer Composition on Release of BSA</title>
<author><name sortKey="Ghassemi, Amir H" sort="Ghassemi, Amir H" uniqKey="Ghassemi A" first="Amir H." last="Ghassemi">Amir H. Ghassemi</name>
<affiliation wicri:level="1"><nlm:aff id="Aff1">Department of Pharmaceutics, Utrecht Institute for Pharmaceutical Sciences (UIPS), Utrecht University, P.O. Box 80082, Sorbonnelaan 16, 3508 TB Utrecht, The Netherlands</nlm:aff>
<country xml:lang="fr">Pays-Bas</country>
<wicri:regionArea>Department of Pharmaceutics, Utrecht Institute for Pharmaceutical Sciences (UIPS), Utrecht University, P.O. Box 80082, Sorbonnelaan 16, 3508 TB Utrecht</wicri:regionArea>
</affiliation>
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<author><name sortKey="Van Steenbergen, Mies J" sort="Van Steenbergen, Mies J" uniqKey="Van Steenbergen M" first="Mies J." last="Van Steenbergen">Mies J. Van Steenbergen</name>
<affiliation wicri:level="1"><nlm:aff id="Aff1">Department of Pharmaceutics, Utrecht Institute for Pharmaceutical Sciences (UIPS), Utrecht University, P.O. Box 80082, Sorbonnelaan 16, 3508 TB Utrecht, The Netherlands</nlm:aff>
<country xml:lang="fr">Pays-Bas</country>
<wicri:regionArea>Department of Pharmaceutics, Utrecht Institute for Pharmaceutical Sciences (UIPS), Utrecht University, P.O. Box 80082, Sorbonnelaan 16, 3508 TB Utrecht</wicri:regionArea>
</affiliation>
</author>
<author><name sortKey="Talsma, Herre" sort="Talsma, Herre" uniqKey="Talsma H" first="Herre" last="Talsma">Herre Talsma</name>
<affiliation wicri:level="1"><nlm:aff id="Aff1">Department of Pharmaceutics, Utrecht Institute for Pharmaceutical Sciences (UIPS), Utrecht University, P.O. Box 80082, Sorbonnelaan 16, 3508 TB Utrecht, The Netherlands</nlm:aff>
<country xml:lang="fr">Pays-Bas</country>
<wicri:regionArea>Department of Pharmaceutics, Utrecht Institute for Pharmaceutical Sciences (UIPS), Utrecht University, P.O. Box 80082, Sorbonnelaan 16, 3508 TB Utrecht</wicri:regionArea>
</affiliation>
</author>
<author><name sortKey="Van Nostrum, Cornelus F" sort="Van Nostrum, Cornelus F" uniqKey="Van Nostrum C" first="Cornelus F." last="Van Nostrum">Cornelus F. Van Nostrum</name>
<affiliation wicri:level="1"><nlm:aff id="Aff1">Department of Pharmaceutics, Utrecht Institute for Pharmaceutical Sciences (UIPS), Utrecht University, P.O. Box 80082, Sorbonnelaan 16, 3508 TB Utrecht, The Netherlands</nlm:aff>
<country xml:lang="fr">Pays-Bas</country>
<wicri:regionArea>Department of Pharmaceutics, Utrecht Institute for Pharmaceutical Sciences (UIPS), Utrecht University, P.O. Box 80082, Sorbonnelaan 16, 3508 TB Utrecht</wicri:regionArea>
</affiliation>
</author>
<author><name sortKey="Crommelin, Daan J A" sort="Crommelin, Daan J A" uniqKey="Crommelin D" first="Daan J. A." last="Crommelin">Daan J. A. Crommelin</name>
<affiliation wicri:level="1"><nlm:aff id="Aff1">Department of Pharmaceutics, Utrecht Institute for Pharmaceutical Sciences (UIPS), Utrecht University, P.O. Box 80082, Sorbonnelaan 16, 3508 TB Utrecht, The Netherlands</nlm:aff>
<country xml:lang="fr">Pays-Bas</country>
<wicri:regionArea>Department of Pharmaceutics, Utrecht Institute for Pharmaceutical Sciences (UIPS), Utrecht University, P.O. Box 80082, Sorbonnelaan 16, 3508 TB Utrecht</wicri:regionArea>
</affiliation>
</author>
<author><name sortKey="Hennink, Wim E" sort="Hennink, Wim E" uniqKey="Hennink W" first="Wim E." last="Hennink">Wim E. Hennink</name>
<affiliation wicri:level="1"><nlm:aff id="Aff1">Department of Pharmaceutics, Utrecht Institute for Pharmaceutical Sciences (UIPS), Utrecht University, P.O. Box 80082, Sorbonnelaan 16, 3508 TB Utrecht, The Netherlands</nlm:aff>
<country xml:lang="fr">Pays-Bas</country>
<wicri:regionArea>Department of Pharmaceutics, Utrecht Institute for Pharmaceutical Sciences (UIPS), Utrecht University, P.O. Box 80082, Sorbonnelaan 16, 3508 TB Utrecht</wicri:regionArea>
</affiliation>
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<series><title level="j">Pharmaceutical Research</title>
<idno type="ISSN">0724-8741</idno>
<idno type="eISSN">1573-904X</idno>
<imprint><date when="2010">2010</date>
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<front><div type="abstract" xml:lang="en"><title>ABSTRACT</title>
<sec><title>Purpose</title>
<p>To study the release of a model protein, bovine serum albumin (BSA), from microspheres of an hydroxylated aliphatic polyester, poly(lactic-co-hydroxymethyl glycolic acid) (PLHMGA).</p>
</sec>
<sec><title>Methods</title>
<p>BSA-loaded microspheres were prepared by a double emulsion solvent evaporation method. The effect of copolymer composition and the molecular weight of the copolymer on <italic>in vitro</italic>
release and degradation were studied. The integrity of the released BSA was studied by fluorescence spectroscopy and size exclusion chromatography (SEC).</p>
</sec>
<sec><title>Results</title>
<p>Microspheres prepared from PLHMGA with 50% hydroxymethyl glycolic acid (HMG) showed a burst release followed by a sustained release in 5–10 days. PLHMGA microspheres prepared from a copolymer with 35% and 25% HMG showed a sustained release of BSA up to 80% for 30 and 60 days, respectively. The release of BSA was hardly affected by the molecular weight of the polymer. Fluorescence spectroscopy and SEC showed that the released BSA preserved its structural integrity. Microspheres were fully degradable, and the degradation time increased from ~20 days to 60 days when the HMG content decreased from 50% to 25%.</p>
</sec>
<sec><title>Conclusions</title>
<p>Taking the degradation and release data together, it can be concluded that the release of BSA from PLHMGA microspheres is governed by degradation of the microspheres.</p>
</sec>
</div>
</front>
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<pmc article-type="research-article"><pmc-dir>properties open_access</pmc-dir>
<front><journal-meta><journal-id journal-id-type="nlm-ta">Pharm Res</journal-id>
<journal-title-group><journal-title>Pharmaceutical Research</journal-title>
</journal-title-group>
<issn pub-type="ppub">0724-8741</issn>
<issn pub-type="epub">1573-904X</issn>
<publisher><publisher-name>Springer US</publisher-name>
<publisher-loc>Boston</publisher-loc>
</publisher>
</journal-meta>
<article-meta><article-id pub-id-type="pmid">20602152</article-id>
<article-id pub-id-type="pmc">2916118</article-id>
<article-id pub-id-type="publisher-id">205</article-id>
<article-id pub-id-type="doi">10.1007/s11095-010-0205-8</article-id>
<article-categories><subj-group subj-group-type="heading"><subject>Research Paper</subject>
</subj-group>
</article-categories>
<title-group><article-title>Hydrophilic Polyester Microspheres: Effect of Molecular Weight and Copolymer Composition on Release of BSA</article-title>
</title-group>
<contrib-group><contrib contrib-type="author"><name><surname>Ghassemi</surname>
<given-names>Amir H.</given-names>
</name>
<xref ref-type="aff" rid="Aff1"></xref>
</contrib>
<contrib contrib-type="author"><name><surname>van Steenbergen</surname>
<given-names>Mies J.</given-names>
</name>
<xref ref-type="aff" rid="Aff1"></xref>
</contrib>
<contrib contrib-type="author"><name><surname>Talsma</surname>
<given-names>Herre</given-names>
</name>
<xref ref-type="aff" rid="Aff1"></xref>
</contrib>
<contrib contrib-type="author"><name><surname>van Nostrum</surname>
<given-names>Cornelus F.</given-names>
</name>
<xref ref-type="aff" rid="Aff1"></xref>
</contrib>
<contrib contrib-type="author"><name><surname>Crommelin</surname>
<given-names>Daan J. A.</given-names>
</name>
<xref ref-type="aff" rid="Aff1"></xref>
</contrib>
<contrib contrib-type="author" corresp="yes"><name><surname>Hennink</surname>
<given-names>Wim E.</given-names>
</name>
<address><phone>+31-30-2536964</phone>
<fax>+31-30-2517839</fax>
<email>w.e.hennink@uu.nl</email>
</address>
<xref ref-type="aff" rid="Aff1"></xref>
</contrib>
<aff id="Aff1">Department of Pharmaceutics, Utrecht Institute for Pharmaceutical Sciences (UIPS), Utrecht University, P.O. Box 80082, Sorbonnelaan 16, 3508 TB Utrecht, The Netherlands</aff>
</contrib-group>
<pub-date pub-type="epub"><day>3</day>
<month>7</month>
<year>2010</year>
</pub-date>
<pub-date pub-type="pmc-release"><day>3</day>
<month>7</month>
<year>2010</year>
</pub-date>
<pub-date pub-type="ppub"><month>9</month>
<year>2010</year>
</pub-date>
<volume>27</volume>
<issue>9</issue>
<fpage>2008</fpage>
<lpage>2017</lpage>
<history><date date-type="received"><day>31</day>
<month>5</month>
<year>2010</year>
</date>
<date date-type="accepted"><day>23</day>
<month>6</month>
<year>2010</year>
</date>
</history>
<permissions><copyright-statement>© The Author(s) 2010</copyright-statement>
</permissions>
<abstract><title>ABSTRACT</title>
<sec><title>Purpose</title>
<p>To study the release of a model protein, bovine serum albumin (BSA), from microspheres of an hydroxylated aliphatic polyester, poly(lactic-co-hydroxymethyl glycolic acid) (PLHMGA).</p>
</sec>
<sec><title>Methods</title>
<p>BSA-loaded microspheres were prepared by a double emulsion solvent evaporation method. The effect of copolymer composition and the molecular weight of the copolymer on <italic>in vitro</italic>
release and degradation were studied. The integrity of the released BSA was studied by fluorescence spectroscopy and size exclusion chromatography (SEC).</p>
</sec>
<sec><title>Results</title>
<p>Microspheres prepared from PLHMGA with 50% hydroxymethyl glycolic acid (HMG) showed a burst release followed by a sustained release in 5–10 days. PLHMGA microspheres prepared from a copolymer with 35% and 25% HMG showed a sustained release of BSA up to 80% for 30 and 60 days, respectively. The release of BSA was hardly affected by the molecular weight of the polymer. Fluorescence spectroscopy and SEC showed that the released BSA preserved its structural integrity. Microspheres were fully degradable, and the degradation time increased from ~20 days to 60 days when the HMG content decreased from 50% to 25%.</p>
</sec>
<sec><title>Conclusions</title>
<p>Taking the degradation and release data together, it can be concluded that the release of BSA from PLHMGA microspheres is governed by degradation of the microspheres.</p>
</sec>
</abstract>
<kwd-group><title>KEY WORDS</title>
<kwd>hydroxylated aliphatic polyester</kwd>
<kwd>microspheres</kwd>
<kwd>PLGA</kwd>
<kwd>protein stability</kwd>
<kwd>release</kwd>
</kwd-group>
<custom-meta-group><custom-meta><meta-name>issue-copyright-statement</meta-name>
<meta-value>© Springer Science+Business Media, LLC 2010</meta-value>
</custom-meta>
</custom-meta-group>
</article-meta>
</front>
</pmc>
</record>
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