Pooled HIV-1 Viral Load Testing Using Dried Blood Spots to Reduce the Cost of Monitoring Antiretroviral Treatment in a Resource-Limited Setting
Identifieur interne : 000251 ( Pmc/Checkpoint ); précédent : 000250; suivant : 000252Pooled HIV-1 Viral Load Testing Using Dried Blood Spots to Reduce the Cost of Monitoring Antiretroviral Treatment in a Resource-Limited Setting
Auteurs : Pieter Pannus [Afrique du Sud] ; Emmanuel Fajardo [Afrique du Sud] ; Carol Metcalf [Afrique du Sud] ; Rebecca M. Coulborn [Malawi] ; Laura T. Durán [Malawi] ; Helen Bygrave [Afrique du Sud] ; Tom Ellman [Afrique du Sud] ; Daniela Garone [Malawi] ; Michael Murowa [Malawi] ; Reuben Mwenda [Malawi] ; Tony Reid [Belgique] ; Wolfgang Preiser [Afrique du Sud]Source :
- Journal of Acquired Immune Deficiency Syndromes (1999) [ 1525-4135 ] ; 2013.
Abstract
Rollout of routine HIV-1 viral load monitoring is hampered by high costs and logistical difficulties associated with sample collection and transport. New strategies are needed to overcome these constraints. Dried blood spots from finger pricks have been shown to be more practical than the use of plasma specimens, and pooling strategies using plasma specimens have been demonstrated to be an efficient method to reduce costs. This study found that combination of finger-prick dried blood spots and a pooling strategy is a feasible and efficient option to reduce costs, while maintaining accuracy in the context of a district hospital in Malawi.
Url:
DOI: 10.1097/QAI.0b013e3182a61e63
PubMed: 23892241
PubMed Central: 3815087
Affiliations:
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PMC:3815087Le document en format XML
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<p>Rollout of routine HIV-1 viral load monitoring is hampered by high costs and logistical difficulties associated with sample collection and transport. New strategies are needed to overcome these constraints. Dried blood spots from finger pricks have been shown to be more practical than the use of plasma specimens, and pooling strategies using plasma specimens have been demonstrated to be an efficient method to reduce costs. This study found that combination of finger-prick dried blood spots and a pooling strategy is a feasible and efficient option to reduce costs, while maintaining accuracy in the context of a district hospital in Malawi.</p>
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<front><journal-meta><journal-id journal-id-type="nlm-ta">J Acquir Immune Defic Syndr</journal-id>
<journal-id journal-id-type="iso-abbrev">J. Acquir. Immune Defic. Syndr</journal-id>
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<issn pub-type="epub">1944-7884</issn>
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<article-id pub-id-type="art-access-id">00003</article-id>
<article-categories><subj-group subj-group-type="heading"><subject>Rapid Communication</subject>
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<title-group><article-title>Pooled HIV-1 Viral Load Testing Using Dried Blood Spots to Reduce the Cost of Monitoring Antiretroviral Treatment in a Resource-Limited Setting</article-title>
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<contrib-group><contrib contrib-type="author"><name><surname>Pannus</surname>
<given-names>Pieter</given-names>
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<degrees>MSc</degrees>
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<contrib contrib-type="author" corresp="yes"><name><surname>Fajardo</surname>
<given-names>Emmanuel</given-names>
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<degrees>BSc</degrees>
<xref ref-type="aff" rid="aff1">*</xref>
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<given-names>Carol</given-names>
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<given-names>Rebecca M.</given-names>
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<degrees>MPH</degrees>
<xref ref-type="aff" rid="aff2">†</xref>
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<given-names>Laura T.</given-names>
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<degrees>MBChB</degrees>
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<given-names>Helen</given-names>
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<contrib contrib-type="author"><name><surname>Ellman</surname>
<given-names>Tom</given-names>
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<degrees>BSc, MBChB, MSc</degrees>
<xref ref-type="aff" rid="aff1">*</xref>
</contrib>
<contrib contrib-type="author"><name><surname>Garone</surname>
<given-names>Daniela</given-names>
</name>
<degrees>MBChB, BSc (ID)</degrees>
<xref ref-type="aff" rid="aff2">†</xref>
</contrib>
<contrib contrib-type="author"><name><surname>Murowa</surname>
<given-names>Michael</given-names>
</name>
<degrees>MBBS</degrees>
<xref ref-type="aff" rid="aff3">‡</xref>
</contrib>
<contrib contrib-type="author"><name><surname>Mwenda</surname>
<given-names>Reuben</given-names>
</name>
<degrees>MSc</degrees>
<xref ref-type="aff" rid="aff4">§</xref>
</contrib>
<contrib contrib-type="author"><name><surname>Reid</surname>
<given-names>Tony</given-names>
</name>
<degrees>MD</degrees>
<xref ref-type="aff" rid="aff5">‖</xref>
</contrib>
<contrib contrib-type="author"><name><surname>Preiser</surname>
<given-names>Wolfgang</given-names>
</name>
<degrees>MD, PhD</degrees>
<xref ref-type="aff" rid="aff6">¶</xref>
</contrib>
<aff id="aff1"><label>*</label>
Médecins Sans Frontières, Southern Africa Medical Unit, Cape Town, South Africa;</aff>
<aff id="aff2"><label>†</label>
Médecins Sans Frontières, Thyolo, Malawi;</aff>
<aff id="aff3"><label>‡</label>
Ministry of Health, Health Technical Support Services, Diagnostics, District Management Office, Thyolo, Malawi;</aff>
<aff id="aff4"><label>§</label>
Ministry of Health, Lilongwe, Malawi;</aff>
<aff id="aff5"><label>‖</label>
Médecins Sans Frontières, Operational Research Unit, Brussels, Belgium; and</aff>
<aff id="aff6"><label>¶</label>
Division of Medical Virology, Stellenbosch University, and National Health Laboratory Service, Tygerberg, South Africa.</aff>
</contrib-group>
<author-notes><corresp>Correspondence to: Emmanuel Fajardo, BSc, Médecins Sans Frontières, Southern Africa Medical Unit, Cape Town, Mowbray, 7925, Wyecroft Road, South Africa (e-mail: <email>emmanuel.fajardo@joburg.msf.org</email>
).</corresp>
</author-notes>
<pub-date pub-type="ppub"><day>1</day>
<month>10</month>
<year>2013</year>
</pub-date>
<pub-date pub-type="epub"><day>16</day>
<month>9</month>
<year>2013</year>
</pub-date>
<volume>64</volume>
<issue>2</issue>
<fpage>134</fpage>
<lpage>137</lpage>
<history><date date-type="received"><day>03</day>
<month>5</month>
<year>2013</year>
</date>
<date date-type="accepted"><day>22</day>
<month>7</month>
<year>2013</year>
</date>
</history>
<permissions><copyright-statement>Copyright © 2013 by Lippincott Williams & Wilkins</copyright-statement>
<copyright-year>2013</copyright-year>
<license license-type="open-access" xlink:href="http://creativecommons.org/licenses/by-nc-nd/3.0"><license-p>This is an open-access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivitives 3.0 License, where it is permissible to download and share the work provided it is properly cited. The work cannot be changed in any way or used commercially.</license-p>
</license>
</permissions>
<self-uri xlink:type="simple" xlink:href="qai-64-134.pdf"></self-uri>
<abstract><title>Abstract:</title>
<p>Rollout of routine HIV-1 viral load monitoring is hampered by high costs and logistical difficulties associated with sample collection and transport. New strategies are needed to overcome these constraints. Dried blood spots from finger pricks have been shown to be more practical than the use of plasma specimens, and pooling strategies using plasma specimens have been demonstrated to be an efficient method to reduce costs. This study found that combination of finger-prick dried blood spots and a pooling strategy is a feasible and efficient option to reduce costs, while maintaining accuracy in the context of a district hospital in Malawi.</p>
</abstract>
<kwd-group><title>Key Words:</title>
<kwd>HIV</kwd>
<kwd>viral load</kwd>
<kwd>pooling</kwd>
<kwd>DBS</kwd>
<kwd>resource-limited setting</kwd>
<kwd>finger prick</kwd>
<kwd>operational research</kwd>
</kwd-group>
<custom-meta-group><custom-meta><meta-name>OPEN-ACCESS</meta-name>
<meta-value>TRUE</meta-value>
</custom-meta>
</custom-meta-group>
</article-meta>
</front>
</pmc>
<affiliations><list><country><li>Afrique du Sud</li>
<li>Belgique</li>
<li>Malawi</li>
</country>
<region><li>Région de Bruxelles-Capitale</li>
</region>
<settlement><li>Bruxelles</li>
</settlement>
</list>
<tree><country name="Afrique du Sud"><noRegion><name sortKey="Pannus, Pieter" sort="Pannus, Pieter" uniqKey="Pannus P" first="Pieter" last="Pannus">Pieter Pannus</name>
</noRegion>
<name sortKey="Bygrave, Helen" sort="Bygrave, Helen" uniqKey="Bygrave H" first="Helen" last="Bygrave">Helen Bygrave</name>
<name sortKey="Ellman, Tom" sort="Ellman, Tom" uniqKey="Ellman T" first="Tom" last="Ellman">Tom Ellman</name>
<name sortKey="Fajardo, Emmanuel" sort="Fajardo, Emmanuel" uniqKey="Fajardo E" first="Emmanuel" last="Fajardo">Emmanuel Fajardo</name>
<name sortKey="Metcalf, Carol" sort="Metcalf, Carol" uniqKey="Metcalf C" first="Carol" last="Metcalf">Carol Metcalf</name>
<name sortKey="Preiser, Wolfgang" sort="Preiser, Wolfgang" uniqKey="Preiser W" first="Wolfgang" last="Preiser">Wolfgang Preiser</name>
</country>
<country name="Malawi"><noRegion><name sortKey="Coulborn, Rebecca M" sort="Coulborn, Rebecca M" uniqKey="Coulborn R" first="Rebecca M." last="Coulborn">Rebecca M. Coulborn</name>
</noRegion>
<name sortKey="Duran, Laura T" sort="Duran, Laura T" uniqKey="Duran L" first="Laura T." last="Durán">Laura T. Durán</name>
<name sortKey="Garone, Daniela" sort="Garone, Daniela" uniqKey="Garone D" first="Daniela" last="Garone">Daniela Garone</name>
<name sortKey="Murowa, Michael" sort="Murowa, Michael" uniqKey="Murowa M" first="Michael" last="Murowa">Michael Murowa</name>
<name sortKey="Mwenda, Reuben" sort="Mwenda, Reuben" uniqKey="Mwenda R" first="Reuben" last="Mwenda">Reuben Mwenda</name>
</country>
<country name="Belgique"><region name="Région de Bruxelles-Capitale"><name sortKey="Reid, Tony" sort="Reid, Tony" uniqKey="Reid T" first="Tony" last="Reid">Tony Reid</name>
</region>
</country>
</tree>
</affiliations>
</record>
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