Biofabrication Under Fluorocarbon: A Novel Freeform Fabrication Technique to Generate High Aspect Ratio Tissue-Engineered Constructs
Identifieur interne : 000203 ( Ncbi/Merge ); précédent : 000202; suivant : 000204Biofabrication Under Fluorocarbon: A Novel Freeform Fabrication Technique to Generate High Aspect Ratio Tissue-Engineered Constructs
Auteurs : Andreas Blaeser ; Daniela F. Duarte Campos ; Michael Weber ; Sabine Neuss ; Benjamin Theek ; Horst Fischer ; Willi Jahnen-DechentSource :
- BioResearch Open Access [ 2164-7844 ] ; 2013.
Abstract
Bioprinting is a recent development in tissue engineering, which applies rapid prototyping techniques to generate complex living tissues. Typically, cell-containing hydrogels are dispensed layer-by-layer according to a computer-generated three-dimensional model. The lack of mechanical stability of printed hydrogels hinders the fabrication of high aspect ratio constructs. Here we present submerged bioprinting, a novel technique for freeform fabrication of hydrogels in liquid fluorocarbon. The high buoyant density of fluorocarbons supports soft hydrogels by floating. Hydrogel constructs of up to 30-mm height were generated. Using 3% (w/v) agarose as the hydrogel and disposable syringe needles as nozzles, the printer produced features down to 570-μm diameter with a lateral dispensing accuracy of 89 μm. We printed thin-walled hydrogel cylinders measuring 4.8 mm in height, with an inner diameter of ∼2.9 mm and a minimal wall thickness of ∼650 μm. The technique was successfully applied in printing a model of an arterial bifurcation. We extruded under fluorocarbon, cellularized alginate tubes with 5-mm outer diameter and 3-cm length. Cells grew vigorously and formed clonal colonies within the 7-day culture period. Submerged bioprinting thus seems particularly suited to fabricate hollow structures with a high aspect ratio like vascular grafts for cardiovascular tissue engineering as well as branching or cantilever-like structures, obviating the need for a solid support beneath the overhanging protrusions.
Url:
DOI: 10.1089/biores.2013.0031
PubMed: 24083093
PubMed Central: 3776616
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<front><div type="abstract" xml:lang="en"><title>Abstract</title>
<p>Bioprinting is a recent development in tissue engineering, which applies rapid prototyping techniques to generate complex living tissues. Typically, cell-containing hydrogels are dispensed layer-by-layer according to a computer-generated three-dimensional model. The lack of mechanical stability of printed hydrogels hinders the fabrication of high aspect ratio constructs. Here we present submerged bioprinting, a novel technique for freeform fabrication of hydrogels in liquid fluorocarbon. The high buoyant density of fluorocarbons supports soft hydrogels by floating. Hydrogel constructs of up to 30-mm height were generated. Using 3% (w/v) agarose as the hydrogel and disposable syringe needles as nozzles, the printer produced features down to 570-μm diameter with a lateral dispensing accuracy of 89 μm. We printed thin-walled hydrogel cylinders measuring 4.8 mm in height, with an inner diameter of ∼2.9 mm and a minimal wall thickness of ∼650 μm. The technique was successfully applied in printing a model of an arterial bifurcation. We extruded under fluorocarbon, cellularized alginate tubes with 5-mm outer diameter and 3-cm length. Cells grew vigorously and formed clonal colonies within the 7-day culture period. Submerged bioprinting thus seems particularly suited to fabricate hollow structures with a high aspect ratio like vascular grafts for cardiovascular tissue engineering as well as branching or cantilever-like structures, obviating the need for a solid support beneath the overhanging protrusions.</p>
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<front><journal-meta><journal-id journal-id-type="nlm-ta">Biores Open Access</journal-id>
<journal-id journal-id-type="iso-abbrev">Biores Open Access</journal-id>
<journal-id journal-id-type="publisher-id">biores</journal-id>
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<publisher-loc>140 Huguenot Street, 3rd FloorNew Rochelle, NY 10801USA</publisher-loc>
</publisher>
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<article-meta><article-id pub-id-type="pmid">24083093</article-id>
<article-id pub-id-type="pmc">3776616</article-id>
<article-id pub-id-type="publisher-id">10.1089/biores.2013.0031</article-id>
<article-id pub-id-type="doi">10.1089/biores.2013.0031</article-id>
<article-categories><subj-group subj-group-type="heading"><subject>Original Research Articles</subject>
</subj-group>
</article-categories>
<title-group><article-title>Biofabrication Under Fluorocarbon: A Novel Freeform Fabrication Technique to Generate High Aspect Ratio Tissue-Engineered Constructs</article-title>
</title-group>
<contrib-group><contrib contrib-type="author"><name><surname>Blaeser</surname>
<given-names>Andreas</given-names>
</name>
<xref ref-type="aff" rid="aff1"><sup>1</sup>
</xref>
<xref ref-type="aff" rid="aff2"><sup>2</sup>
</xref>
</contrib>
<contrib contrib-type="author"><name><surname>Duarte Campos</surname>
<given-names>Daniela F.</given-names>
</name>
<xref ref-type="aff" rid="aff2"><sup>2</sup>
</xref>
</contrib>
<contrib contrib-type="author"><name><surname>Weber</surname>
<given-names>Michael</given-names>
</name>
<xref ref-type="aff" rid="aff2"><sup>2</sup>
</xref>
</contrib>
<contrib contrib-type="author"><name><surname>Neuss</surname>
<given-names>Sabine</given-names>
</name>
<xref ref-type="aff" rid="aff1"><sup>1</sup>
</xref>
<xref ref-type="aff" rid="aff3"><sup>3</sup>
</xref>
</contrib>
<contrib contrib-type="author"><name><surname>Theek</surname>
<given-names>Benjamin</given-names>
</name>
<xref ref-type="aff" rid="aff4"><sup>4</sup>
</xref>
</contrib>
<contrib contrib-type="author"><name><surname>Fischer</surname>
<given-names>Horst</given-names>
</name>
<xref ref-type="aff" rid="aff2"><sup>2</sup>
</xref>
</contrib>
<contrib contrib-type="author" corresp="yes"><name><surname>Jahnen-Dechent</surname>
<given-names>Willi</given-names>
</name>
<xref ref-type="aff" rid="aff1"><sup>1</sup>
</xref>
</contrib>
<aff id="aff1"><label><sup>1</sup>
</label>
Biointerface Laboratory,<institution>RWTH Aachen University Hospital</institution>
, Aachen,<country>Germany</country>
.</aff>
<aff id="aff2"><label><sup>2</sup>
</label>
Department of Dental Materials and Biomaterial Research,<institution>RWTH Aachen University Hospital</institution>
, Aachen,<country>Germany</country>
.</aff>
<aff id="aff3"><label><sup>3</sup>
</label>
Institute of Pathology,<institution>RWTH Aachen University Hospital</institution>
, Aachen,<country>Germany</country>
.</aff>
<aff id="aff4"><label><sup>4</sup>
</label>
Experimental Molecular Imaging, Helmholtz Institute of Biomedical Engineering,<institution>RWTH Aachen University Hospital</institution>
, Aachen,<country>Germany</country>
.</aff>
</contrib-group>
<author-notes><corresp>Address correspondence to: <italic>Willi Jahnen-Dechent, PhD, Helmholtz Institute of Biomedical Engineering, Biointerface Laboratory, RWTH Aachen University Hospital, Pauwelsstrasse 30, 52074 Aachen, Germany. E-mail:</italic>
<email xlink:href="mailto:willi.jahnen@rwth-aachen.de">willi.jahnen@rwth-aachen.de</email>
</corresp>
</author-notes>
<pub-date pub-type="ppub"><month>10</month>
<year>2013</year>
<pmc-comment>string-date: October 2013</pmc-comment>
</pub-date>
<pub-date pub-type="pmc-release"><month>10</month>
<year>2013</year>
<pmc-comment>string-date: October 2013</pmc-comment>
</pub-date>
<pmc-comment> PMC Release delay is 0 months and 0 days and was based on the
. </pmc-comment>
<volume>2</volume>
<issue>5</issue>
<fpage>374</fpage>
<lpage>384</lpage>
<permissions><copyright-statement>Copyright 2013, Mary Ann Liebert, Inc.</copyright-statement>
<copyright-year>2013</copyright-year>
</permissions>
<self-uri xlink:type="simple" xlink:href="biores.2013.0031.pdf"></self-uri>
<abstract><title>Abstract</title>
<p>Bioprinting is a recent development in tissue engineering, which applies rapid prototyping techniques to generate complex living tissues. Typically, cell-containing hydrogels are dispensed layer-by-layer according to a computer-generated three-dimensional model. The lack of mechanical stability of printed hydrogels hinders the fabrication of high aspect ratio constructs. Here we present submerged bioprinting, a novel technique for freeform fabrication of hydrogels in liquid fluorocarbon. The high buoyant density of fluorocarbons supports soft hydrogels by floating. Hydrogel constructs of up to 30-mm height were generated. Using 3% (w/v) agarose as the hydrogel and disposable syringe needles as nozzles, the printer produced features down to 570-μm diameter with a lateral dispensing accuracy of 89 μm. We printed thin-walled hydrogel cylinders measuring 4.8 mm in height, with an inner diameter of ∼2.9 mm and a minimal wall thickness of ∼650 μm. The technique was successfully applied in printing a model of an arterial bifurcation. We extruded under fluorocarbon, cellularized alginate tubes with 5-mm outer diameter and 3-cm length. Cells grew vigorously and formed clonal colonies within the 7-day culture period. Submerged bioprinting thus seems particularly suited to fabricate hollow structures with a high aspect ratio like vascular grafts for cardiovascular tissue engineering as well as branching or cantilever-like structures, obviating the need for a solid support beneath the overhanging protrusions.</p>
</abstract>
<kwd-group kwd-group-type="author"><title>Keywords</title>
<kwd>biomaterials</kwd>
<kwd>cardiology</kwd>
<kwd>cell culture</kwd>
<kwd>tissue engineering</kwd>
</kwd-group>
<counts><fig-count count="10"></fig-count>
<table-count count="3"></table-count>
<equation-count count="1"></equation-count>
<ref-count count="40"></ref-count>
<page-count count="11"></page-count>
</counts>
</article-meta>
</front>
</pmc>
<affiliations><list></list>
<tree><noCountry><name sortKey="Blaeser, Andreas" sort="Blaeser, Andreas" uniqKey="Blaeser A" first="Andreas" last="Blaeser">Andreas Blaeser</name>
<name sortKey="Duarte Campos, Daniela F" sort="Duarte Campos, Daniela F" uniqKey="Duarte Campos D" first="Daniela F." last="Duarte Campos">Daniela F. Duarte Campos</name>
<name sortKey="Fischer, Horst" sort="Fischer, Horst" uniqKey="Fischer H" first="Horst" last="Fischer">Horst Fischer</name>
<name sortKey="Jahnen Dechent, Willi" sort="Jahnen Dechent, Willi" uniqKey="Jahnen Dechent W" first="Willi" last="Jahnen-Dechent">Willi Jahnen-Dechent</name>
<name sortKey="Neuss, Sabine" sort="Neuss, Sabine" uniqKey="Neuss S" first="Sabine" last="Neuss">Sabine Neuss</name>
<name sortKey="Theek, Benjamin" sort="Theek, Benjamin" uniqKey="Theek B" first="Benjamin" last="Theek">Benjamin Theek</name>
<name sortKey="Weber, Michael" sort="Weber, Michael" uniqKey="Weber M" first="Michael" last="Weber">Michael Weber</name>
</noCountry>
</tree>
</affiliations>
</record>
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