Different solutions used for submucosal injection influenced early healing of gastric endoscopic mucosal resection in a preclinical study in experimental pigs
Identifieur interne : 000046 ( Ncbi/Merge ); précédent : 000045; suivant : 000047Different solutions used for submucosal injection influenced early healing of gastric endoscopic mucosal resection in a preclinical study in experimental pigs
Auteurs : Jan Bureš [République tchèque] ; Marcela Kopá Ová [République tchèque] ; Jaroslav Kv Tina [République tchèque] ; Jan Österreicher [République tchèque] ; Zuzana Šinkorová [République tchèque] ; Zbyn K Svoboda [République tchèque] ; Ilja Tachecí [République tchèque] ; Stanislav Filip [République tchèque] ; Stanislav Špelda [République tchèque] ; Martin Kuneš [République tchèque] ; Stanislav Rejchrt [République tchèque]Source :
- Surgical Endoscopy [ 0930-2794 ] ; 2008.
Abstract
We hypothesised that different solutions for submucosal injection may influence early healing of endoscopic mucosal resection (EMR). The aim of this study was to evaluate histological and immunological changes after EMR in experimental pigs.
Two parallel EMRs on the anterior and posterior wall of the gastric body were performed by means of the cap technique in 21 female pigs. A glycerol-based solution (anterior EMR) and hydroxypropyl methylcellulose solution (posterior EMR) were applied for submucosal injection. The animals were sacrificed 7 days later, and tissue sections of all EMRs were stained using combined trichrome. Computer image analysis was used for objective evaluation of elastic and collagen fibres content. Two-colour indirect immunophenotyping of blood and gastric samples were performed using mouse anti-pig monoclonal antibodies.
The values of collagen fibre content 7 days after EMR were significantly higher in lesions after the use of solution A in comparison with solution B (2.10 ± 0.25% versus 1.57 ± 0.25%,
The use of glycerol-based solution for submucosal injection was associated with more pronounced histological signs of early healing of EMRs compared with hydroxypropyl methylcellulose.
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DOI: 10.1007/s00464-008-0207-3
PubMed: 19057952
PubMed Central: 2730453
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uniqKey="Kopa Ova M" first="Marcela" last="Kopá Ová">Marcela Kopá Ová</name><affiliation wicri:level="1"><nlm:aff id="Aff1">Second Department of Internal Medicine, Charles University in Praha, Faculty of Medicine at Hradec Králové, University Teaching Hospital, Sokolská 581, 500 05 Hradec Králové, Czech Republic</nlm:aff><country xml:lang="fr">République tchèque</country><wicri:regionArea>Second Department of Internal Medicine, Charles University in Praha, Faculty of Medicine at Hradec Králové, University Teaching Hospital, Sokolská 581, 500 05 Hradec Králové</wicri:regionArea><wicri:noRegion>500 05 Hradec Králové</wicri:noRegion></affiliation></author><author><name sortKey="Kv Tina, Jaroslav" sort="Kv Tina, Jaroslav" uniqKey="Kv Tina J" first="Jaroslav" last="Kv Tina">Jaroslav Kv Tina</name><affiliation wicri:level="1"><nlm:aff id="Aff2">Institute of Experimental Biopharmaceutics, Joint Research Center of Czech Academy of Sciences and PRO.MED.CS Praha a.s., Hradec Králové, Czech Republic</nlm:aff><country xml:lang="fr">République tchèque</country><wicri:regionArea>Institute of Experimental Biopharmaceutics, Joint Research Center of Czech Academy of Sciences and PRO.MED.CS Praha a.s., Hradec Králové</wicri:regionArea><wicri:noRegion>Hradec Králové</wicri:noRegion></affiliation></author><author><name sortKey="Osterreicher, Jan" sort="Osterreicher, Jan" uniqKey="Osterreicher J" first="Jan" last="Österreicher">Jan Österreicher</name><affiliation wicri:level="1"><nlm:aff id="Aff3">Department of Radiobiology, Faculty of Military Health Sciences, University of Defense, Hradec Králové, Czech Republic</nlm:aff><country xml:lang="fr">République tchèque</country><wicri:regionArea>Department of Radiobiology, Faculty of Military Health Sciences, University of Defense, Hradec Králové</wicri:regionArea><wicri:noRegion>Hradec Králové</wicri:noRegion></affiliation></author><author><name sortKey="Sinkorova, Zuzana" sort="Sinkorova, Zuzana" uniqKey="Sinkorova Z" first="Zuzana" last="Šinkorová">Zuzana Šinkorová</name><affiliation wicri:level="1"><nlm:aff id="Aff3">Department of Radiobiology, Faculty of Military Health Sciences, University of Defense, Hradec Králové, Czech Republic</nlm:aff><country xml:lang="fr">République tchèque</country><wicri:regionArea>Department of Radiobiology, Faculty of Military Health Sciences, University of Defense, Hradec Králové</wicri:regionArea><wicri:noRegion>Hradec Králové</wicri:noRegion></affiliation></author><author><name sortKey="Svoboda, Zbyn K" sort="Svoboda, Zbyn K" uniqKey="Svoboda Z" first="Zbyn K" last="Svoboda">Zbyn K Svoboda</name><affiliation wicri:level="1"><nlm:aff id="Aff2">Institute of Experimental Biopharmaceutics, Joint Research Center of Czech Academy of Sciences and PRO.MED.CS Praha a.s., Hradec Králové, Czech Republic</nlm:aff><country xml:lang="fr">République tchèque</country><wicri:regionArea>Institute of Experimental Biopharmaceutics, Joint Research Center of Czech Academy of Sciences and PRO.MED.CS Praha a.s., Hradec Králové</wicri:regionArea><wicri:noRegion>Hradec Králové</wicri:noRegion></affiliation></author><author><name sortKey="Tacheci, Ilja" sort="Tacheci, Ilja" uniqKey="Tacheci I" first="Ilja" last="Tachecí">Ilja Tachecí</name><affiliation wicri:level="1"><nlm:aff id="Aff1">Second Department of Internal Medicine, Charles University in Praha, Faculty of Medicine at Hradec Králové, University Teaching Hospital, Sokolská 581, 500 05 Hradec Králové, Czech Republic</nlm:aff><country xml:lang="fr">République tchèque</country><wicri:regionArea>Second Department of Internal Medicine, Charles University in Praha, Faculty of Medicine at Hradec Králové, University Teaching Hospital, Sokolská 581, 500 05 Hradec Králové</wicri:regionArea><wicri:noRegion>500 05 Hradec Králové</wicri:noRegion></affiliation></author><author><name sortKey="Filip, Stanislav" sort="Filip, Stanislav" uniqKey="Filip S" first="Stanislav" last="Filip">Stanislav Filip</name><affiliation wicri:level="1"><nlm:aff id="Aff4">Department of Oncology and Radiotherapy, Charles University in Praha, Faculty of Medicine at Hradec Králové, University Teaching Hospital, Hradec Králové, Czech Republic</nlm:aff><country xml:lang="fr">République tchèque</country><wicri:regionArea>Department of Oncology and Radiotherapy, Charles University in Praha, Faculty of Medicine at Hradec Králové, University Teaching Hospital, Hradec Králové</wicri:regionArea><wicri:noRegion>Hradec Králové</wicri:noRegion></affiliation></author><author><name sortKey="Spelda, Stanislav" sort="Spelda, Stanislav" uniqKey="Spelda S" first="Stanislav" last="Špelda">Stanislav Špelda</name><affiliation wicri:level="1"><nlm:aff id="Aff3">Department of Radiobiology, Faculty of Military Health Sciences, University of Defense, Hradec Králové, Czech Republic</nlm:aff><country xml:lang="fr">République tchèque</country><wicri:regionArea>Department of Radiobiology, Faculty of Military Health Sciences, University of Defense, Hradec Králové</wicri:regionArea><wicri:noRegion>Hradec Králové</wicri:noRegion></affiliation></author><author><name sortKey="Kunes, Martin" sort="Kunes, Martin" uniqKey="Kunes M" first="Martin" last="Kuneš">Martin Kuneš</name><affiliation wicri:level="1"><nlm:aff id="Aff2">Institute of Experimental Biopharmaceutics, Joint Research Center of Czech Academy of Sciences and PRO.MED.CS Praha a.s., Hradec Králové, Czech Republic</nlm:aff><country xml:lang="fr">République tchèque</country><wicri:regionArea>Institute of Experimental Biopharmaceutics, Joint Research Center of Czech Academy of Sciences and PRO.MED.CS Praha a.s., Hradec Králové</wicri:regionArea><wicri:noRegion>Hradec Králové</wicri:noRegion></affiliation></author><author><name sortKey="Rejchrt, Stanislav" sort="Rejchrt, Stanislav" uniqKey="Rejchrt S" first="Stanislav" last="Rejchrt">Stanislav Rejchrt</name><affiliation wicri:level="1"><nlm:aff id="Aff1">Second Department of Internal Medicine, Charles University in Praha, Faculty of Medicine at Hradec Králové, University Teaching Hospital, Sokolská 581, 500 05 Hradec Králové, Czech Republic</nlm:aff><country xml:lang="fr">République tchèque</country><wicri:regionArea>Second Department of Internal Medicine, Charles University in Praha, Faculty of Medicine at Hradec Králové, University Teaching Hospital, Sokolská 581, 500 05 Hradec Králové</wicri:regionArea><wicri:noRegion>500 05 Hradec Králové</wicri:noRegion></affiliation></author></titleStmt><publicationStmt><idno type="wicri:source">PMC</idno><idno type="pmid">19057952</idno><idno type="pmc">2730453</idno><idno type="url">http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2730453</idno><idno type="RBID">PMC:2730453</idno><idno type="doi">10.1007/s00464-008-0207-3</idno><date when="2008">2008</date><idno type="wicri:Area/Pmc/Corpus">000247</idno><idno type="wicri:Area/Pmc/Curation">000247</idno><idno type="wicri:Area/Pmc/Checkpoint">000426</idno><idno type="wicri:Area/Ncbi/Merge">000046</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en" level="a" type="main">Different solutions used for submucosal injection influenced early healing of gastric endoscopic mucosal resection in a preclinical study in experimental pigs</title><author><name sortKey="Bures, Jan" sort="Bures, Jan" uniqKey="Bures J" first="Jan" last="Bureš">Jan Bureš</name><affiliation wicri:level="1"><nlm:aff id="Aff1">Second Department of Internal Medicine, Charles University in Praha, Faculty of Medicine at Hradec Králové, University Teaching Hospital, Sokolská 581, 500 05 Hradec Králové, Czech Republic</nlm:aff><country xml:lang="fr">République tchèque</country><wicri:regionArea>Second Department of Internal Medicine, Charles University in Praha, Faculty of Medicine at Hradec Králové, University Teaching Hospital, Sokolská 581, 500 05 Hradec Králové</wicri:regionArea><wicri:noRegion>500 05 Hradec Králové</wicri:noRegion></affiliation></author><author><name sortKey="Kopa Ova, Marcela" sort="Kopa Ova, Marcela" uniqKey="Kopa Ova M" first="Marcela" last="Kopá Ová">Marcela Kopá Ová</name><affiliation wicri:level="1"><nlm:aff id="Aff1">Second Department of Internal Medicine, Charles University in Praha, Faculty of Medicine at Hradec Králové, University Teaching Hospital, Sokolská 581, 500 05 Hradec Králové, Czech Republic</nlm:aff><country xml:lang="fr">République tchèque</country><wicri:regionArea>Second Department of Internal Medicine, Charles University in Praha, Faculty of Medicine at Hradec Králové, University Teaching Hospital, Sokolská 581, 500 05 Hradec Králové</wicri:regionArea><wicri:noRegion>500 05 Hradec Králové</wicri:noRegion></affiliation></author><author><name sortKey="Kv Tina, Jaroslav" sort="Kv Tina, Jaroslav" uniqKey="Kv Tina J" first="Jaroslav" last="Kv Tina">Jaroslav Kv Tina</name><affiliation wicri:level="1"><nlm:aff id="Aff2">Institute of Experimental Biopharmaceutics, Joint Research Center of Czech Academy of Sciences and PRO.MED.CS Praha a.s., Hradec Králové, Czech Republic</nlm:aff><country xml:lang="fr">République tchèque</country><wicri:regionArea>Institute of Experimental Biopharmaceutics, Joint Research Center of Czech Academy of Sciences and PRO.MED.CS Praha a.s., Hradec Králové</wicri:regionArea><wicri:noRegion>Hradec Králové</wicri:noRegion></affiliation></author><author><name sortKey="Osterreicher, Jan" sort="Osterreicher, Jan" uniqKey="Osterreicher J" first="Jan" last="Österreicher">Jan Österreicher</name><affiliation wicri:level="1"><nlm:aff id="Aff3">Department of Radiobiology, Faculty of Military Health Sciences, University of Defense, Hradec Králové, Czech Republic</nlm:aff><country xml:lang="fr">République tchèque</country><wicri:regionArea>Department of Radiobiology, Faculty of Military Health Sciences, University of Defense, Hradec Králové</wicri:regionArea><wicri:noRegion>Hradec Králové</wicri:noRegion></affiliation></author><author><name sortKey="Sinkorova, Zuzana" sort="Sinkorova, Zuzana" uniqKey="Sinkorova Z" first="Zuzana" last="Šinkorová">Zuzana Šinkorová</name><affiliation wicri:level="1"><nlm:aff id="Aff3">Department of Radiobiology, Faculty of Military Health Sciences, University of Defense, Hradec Králové, Czech Republic</nlm:aff><country xml:lang="fr">République tchèque</country><wicri:regionArea>Department of Radiobiology, Faculty of Military Health Sciences, University of Defense, Hradec Králové</wicri:regionArea><wicri:noRegion>Hradec Králové</wicri:noRegion></affiliation></author><author><name sortKey="Svoboda, Zbyn K" sort="Svoboda, Zbyn K" uniqKey="Svoboda Z" first="Zbyn K" last="Svoboda">Zbyn K Svoboda</name><affiliation wicri:level="1"><nlm:aff id="Aff2">Institute of Experimental Biopharmaceutics, Joint Research Center of Czech Academy of Sciences and PRO.MED.CS Praha a.s., Hradec Králové, Czech Republic</nlm:aff><country xml:lang="fr">République tchèque</country><wicri:regionArea>Institute of Experimental Biopharmaceutics, Joint Research Center of Czech Academy of Sciences and PRO.MED.CS Praha a.s., Hradec Králové</wicri:regionArea><wicri:noRegion>Hradec Králové</wicri:noRegion></affiliation></author><author><name sortKey="Tacheci, Ilja" sort="Tacheci, Ilja" uniqKey="Tacheci I" first="Ilja" last="Tachecí">Ilja Tachecí</name><affiliation wicri:level="1"><nlm:aff id="Aff1">Second Department of Internal Medicine, Charles University in Praha, Faculty of Medicine at Hradec Králové, University Teaching Hospital, Sokolská 581, 500 05 Hradec Králové, Czech Republic</nlm:aff><country xml:lang="fr">République tchèque</country><wicri:regionArea>Second Department of Internal Medicine, Charles University in Praha, Faculty of Medicine at Hradec Králové, University Teaching Hospital, Sokolská 581, 500 05 Hradec Králové</wicri:regionArea><wicri:noRegion>500 05 Hradec Králové</wicri:noRegion></affiliation></author><author><name sortKey="Filip, Stanislav" sort="Filip, Stanislav" uniqKey="Filip S" first="Stanislav" last="Filip">Stanislav Filip</name><affiliation wicri:level="1"><nlm:aff id="Aff4">Department of Oncology and Radiotherapy, Charles University in Praha, Faculty of Medicine at Hradec Králové, University Teaching Hospital, Hradec Králové, Czech Republic</nlm:aff><country xml:lang="fr">République tchèque</country><wicri:regionArea>Department of Oncology and Radiotherapy, Charles University in Praha, Faculty of Medicine at Hradec Králové, University Teaching Hospital, Hradec Králové</wicri:regionArea><wicri:noRegion>Hradec Králové</wicri:noRegion></affiliation></author><author><name sortKey="Spelda, Stanislav" sort="Spelda, Stanislav" uniqKey="Spelda S" first="Stanislav" last="Špelda">Stanislav Špelda</name><affiliation wicri:level="1"><nlm:aff id="Aff3">Department of Radiobiology, Faculty of Military Health Sciences, University of Defense, Hradec Králové, Czech Republic</nlm:aff><country xml:lang="fr">République tchèque</country><wicri:regionArea>Department of Radiobiology, Faculty of Military Health Sciences, University of Defense, Hradec Králové</wicri:regionArea><wicri:noRegion>Hradec Králové</wicri:noRegion></affiliation></author><author><name sortKey="Kunes, Martin" sort="Kunes, Martin" uniqKey="Kunes M" first="Martin" last="Kuneš">Martin Kuneš</name><affiliation wicri:level="1"><nlm:aff id="Aff2">Institute of Experimental Biopharmaceutics, Joint Research Center of Czech Academy of Sciences and PRO.MED.CS Praha a.s., Hradec Králové, Czech Republic</nlm:aff><country xml:lang="fr">République tchèque</country><wicri:regionArea>Institute of Experimental Biopharmaceutics, Joint Research Center of Czech Academy of Sciences and PRO.MED.CS Praha a.s., Hradec Králové</wicri:regionArea><wicri:noRegion>Hradec Králové</wicri:noRegion></affiliation></author><author><name sortKey="Rejchrt, Stanislav" sort="Rejchrt, Stanislav" uniqKey="Rejchrt S" first="Stanislav" last="Rejchrt">Stanislav Rejchrt</name><affiliation wicri:level="1"><nlm:aff id="Aff1">Second Department of Internal Medicine, Charles University in Praha, Faculty of Medicine at Hradec Králové, University Teaching Hospital, Sokolská 581, 500 05 Hradec Králové, Czech Republic</nlm:aff><country xml:lang="fr">République tchèque</country><wicri:regionArea>Second Department of Internal Medicine, Charles University in Praha, Faculty of Medicine at Hradec Králové, University Teaching Hospital, Sokolská 581, 500 05 Hradec Králové</wicri:regionArea><wicri:noRegion>500 05 Hradec Králové</wicri:noRegion></affiliation></author></analytic><series><title level="j">Surgical Endoscopy</title><idno type="ISSN">0930-2794</idno><idno type="eISSN">1432-2218</idno><imprint><date when="2008">2008</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en"><sec><title>Background</title><p>We hypothesised that different solutions for submucosal injection may influence early healing of endoscopic mucosal resection (EMR). The aim of this study was to evaluate histological and immunological changes after EMR in experimental pigs.</p></sec><sec><title>Materials and methods</title><p>Two parallel EMRs on the anterior and posterior wall of the gastric body were performed by means of the cap technique in 21 female pigs. A glycerol-based solution (anterior EMR) and hydroxypropyl methylcellulose solution (posterior EMR) were applied for submucosal injection. The animals were sacrificed 7 days later, and tissue sections of all EMRs were stained using combined trichrome. Computer image analysis was used for objective evaluation of elastic and collagen fibres content. Two-colour indirect immunophenotyping of blood and gastric samples were performed using mouse anti-pig monoclonal antibodies.</p></sec><sec><title>Results</title><p>The values of collagen fibre content 7 days after EMR were significantly higher in lesions after the use of solution A in comparison with solution B (2.10 ± 0.25% versus 1.57 ± 0.25%, <italic>p</italic> = 0.009). Concordant results were found in elastic fibres (3.23 ± 0.49% versus 2.93 ± 0.61%, <italic>p</italic> = 0.018). No systemic changes in major leukocyte subpopulations were found. In gastric tissue, lymphocyte subsets exhibited only minor changes. CD4<sup>+</sup> T-lymphocytes were increased in the healing tissue after EMR using solution A (17.08 ± 9.24% versus 9.76 ± 7.97%, <italic>p</italic> = 0.011). Significant increase of SWC3<sup>+</sup> leukocytes was observed after EMR using solution B (47.70 ± 25.41% versus 18.70 ± 12.16%, <italic>p</italic> = 0.001).</p></sec><sec><title>Conclusions</title><p>The use of glycerol-based solution for submucosal injection was associated with more pronounced histological signs of early healing of EMRs compared with hydroxypropyl methylcellulose.</p></sec></div></front><back><div1 type="bibliography"><listBibl><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct></listBibl></div1></back></TEI><pmc xml:lang="EN" article-type="research-article"><pmc-dir>properties open_access</pmc-dir>
<front><journal-meta><journal-id journal-id-type="nlm-ta">Surg Endosc</journal-id><journal-title>Surgical Endoscopy</journal-title><issn pub-type="ppub">0930-2794</issn><issn pub-type="epub">1432-2218</issn><publisher><publisher-name>Springer-Verlag</publisher-name><publisher-loc>New York</publisher-loc></publisher></journal-meta><article-meta><article-id pub-id-type="pmid">19057952</article-id><article-id pub-id-type="pmc">2730453</article-id><article-id pub-id-type="publisher-id">207</article-id><article-id pub-id-type="doi">10.1007/s00464-008-0207-3</article-id><article-categories><subj-group subj-group-type="heading"><subject>Article</subject></subj-group></article-categories><title-group><article-title>Different solutions used for submucosal injection influenced early healing of gastric endoscopic mucosal resection in a preclinical study in experimental pigs</article-title></title-group><contrib-group><contrib contrib-type="author" corresp="yes"><name name-style="western"><surname>Bureš</surname><given-names>Jan</given-names></name><address><email>bures@lfhk.cuni.cz</email></address><xref ref-type="aff" rid="Aff1">1</xref></contrib><contrib contrib-type="author"><name name-style="western"><surname>Kopáčová</surname><given-names>Marcela</given-names></name><xref ref-type="aff" rid="Aff1">1</xref></contrib><contrib contrib-type="author"><name name-style="western"><surname>Květina</surname><given-names>Jaroslav</given-names></name><xref ref-type="aff" rid="Aff2">2</xref></contrib><contrib contrib-type="author"><name name-style="western"><surname>Österreicher</surname><given-names>Jan</given-names></name><xref ref-type="aff" rid="Aff3">3</xref></contrib><contrib contrib-type="author"><name name-style="western"><surname>Šinkorová</surname><given-names>Zuzana</given-names></name><xref ref-type="aff" rid="Aff3">3</xref></contrib><contrib contrib-type="author"><name name-style="western"><surname>Svoboda</surname><given-names>Zbyněk</given-names></name><xref ref-type="aff" rid="Aff2">2</xref></contrib><contrib contrib-type="author"><name name-style="western"><surname>Tachecí</surname><given-names>Ilja</given-names></name><xref ref-type="aff" rid="Aff1">1</xref></contrib><contrib contrib-type="author"><name name-style="western"><surname>Filip</surname><given-names>Stanislav</given-names></name><xref ref-type="aff" rid="Aff4">4</xref></contrib><contrib contrib-type="author"><name name-style="western"><surname>Špelda</surname><given-names>Stanislav</given-names></name><xref ref-type="aff" rid="Aff3">3</xref></contrib><contrib contrib-type="author"><name name-style="western"><surname>Kuneš</surname><given-names>Martin</given-names></name><xref ref-type="aff" rid="Aff2">2</xref></contrib><contrib contrib-type="author"><name name-style="western"><surname>Rejchrt</surname><given-names>Stanislav</given-names></name><xref ref-type="aff" rid="Aff1">1</xref></contrib><aff id="Aff1"><label>1</label>Second Department of Internal Medicine, Charles University in Praha, Faculty of Medicine at Hradec Králové, University Teaching Hospital, Sokolská 581, 500 05 Hradec Králové, Czech Republic</aff><aff id="Aff2"><label>2</label>Institute of Experimental Biopharmaceutics, Joint Research Center of Czech Academy of Sciences and PRO.MED.CS Praha a.s., Hradec Králové, Czech Republic</aff><aff id="Aff3"><label>3</label>Department of Radiobiology, Faculty of Military Health Sciences, University of Defense, Hradec Králové, Czech Republic</aff><aff id="Aff4"><label>4</label>Department of Oncology and Radiotherapy, Charles University in Praha, Faculty of Medicine at Hradec Králové, University Teaching Hospital, Hradec Králové, Czech Republic</aff></contrib-group><pub-date pub-type="epub"><day>5</day><month>12</month><year>2008</year></pub-date><pub-date pub-type="ppub"><month>9</month><year>2009</year></pub-date><volume>23</volume><issue>9</issue><fpage>2094</fpage><lpage>2101</lpage><history><date date-type="received"><day>31</day><month>5</month><year>2008</year></date><date date-type="rev-recd"><day>3</day><month>9</month><year>2008</year></date><date date-type="accepted"><day>6</day><month>10</month><year>2008</year></date></history><permissions><copyright-statement>© The Author(s) 2008</copyright-statement></permissions><abstract xml:lang="EN"><sec><title>Background</title><p>We hypothesised that different solutions for submucosal injection may influence early healing of endoscopic mucosal resection (EMR). The aim of this study was to evaluate histological and immunological changes after EMR in experimental pigs.</p></sec><sec><title>Materials and methods</title><p>Two parallel EMRs on the anterior and posterior wall of the gastric body were performed by means of the cap technique in 21 female pigs. A glycerol-based solution (anterior EMR) and hydroxypropyl methylcellulose solution (posterior EMR) were applied for submucosal injection. The animals were sacrificed 7 days later, and tissue sections of all EMRs were stained using combined trichrome. Computer image analysis was used for objective evaluation of elastic and collagen fibres content. Two-colour indirect immunophenotyping of blood and gastric samples were performed using mouse anti-pig monoclonal antibodies.</p></sec><sec><title>Results</title><p>The values of collagen fibre content 7 days after EMR were significantly higher in lesions after the use of solution A in comparison with solution B (2.10 ± 0.25% versus 1.57 ± 0.25%, <italic>p</italic> = 0.009). Concordant results were found in elastic fibres (3.23 ± 0.49% versus 2.93 ± 0.61%, <italic>p</italic> = 0.018). No systemic changes in major leukocyte subpopulations were found. In gastric tissue, lymphocyte subsets exhibited only minor changes. CD4<sup>+</sup> T-lymphocytes were increased in the healing tissue after EMR using solution A (17.08 ± 9.24% versus 9.76 ± 7.97%, <italic>p</italic> = 0.011). Significant increase of SWC3<sup>+</sup> leukocytes was observed after EMR using solution B (47.70 ± 25.41% versus 18.70 ± 12.16%, <italic>p</italic> = 0.001).</p></sec><sec><title>Conclusions</title><p>The use of glycerol-based solution for submucosal injection was associated with more pronounced histological signs of early healing of EMRs compared with hydroxypropyl methylcellulose.</p></sec></abstract><kwd-group><title>Keywords</title><kwd>Submucosal injection</kwd><kwd>Healing</kwd><kwd>Gastric endoscopic mucosal resection</kwd><kwd>Experimental pigs</kwd></kwd-group><custom-meta-wrap><custom-meta><meta-name>issue-copyright-statement</meta-name><meta-value>© Springer Science+Business Media, LLC 2009</meta-value></custom-meta></custom-meta-wrap></article-meta></front></pmc><affiliations><list><country><li>République tchèque</li></country></list><tree><country name="République tchèque"><noRegion><name sortKey="Bures, Jan" sort="Bures, Jan" uniqKey="Bures J" first="Jan" last="Bureš">Jan Bureš</name></noRegion><name sortKey="Filip, Stanislav" sort="Filip, Stanislav" uniqKey="Filip S" first="Stanislav" last="Filip">Stanislav Filip</name><name sortKey="Kopa Ova, Marcela" sort="Kopa Ova, Marcela" uniqKey="Kopa Ova M" first="Marcela" last="Kopá Ová">Marcela Kopá Ová</name><name sortKey="Kunes, Martin" sort="Kunes, Martin" uniqKey="Kunes M" first="Martin" last="Kuneš">Martin Kuneš</name><name sortKey="Kv Tina, Jaroslav" sort="Kv Tina, Jaroslav" uniqKey="Kv Tina J" first="Jaroslav" last="Kv Tina">Jaroslav Kv Tina</name><name sortKey="Osterreicher, Jan" sort="Osterreicher, Jan" uniqKey="Osterreicher J" first="Jan" last="Österreicher">Jan Österreicher</name><name sortKey="Rejchrt, Stanislav" sort="Rejchrt, Stanislav" uniqKey="Rejchrt S" first="Stanislav" last="Rejchrt">Stanislav Rejchrt</name><name sortKey="Sinkorova, Zuzana" sort="Sinkorova, Zuzana" uniqKey="Sinkorova Z" first="Zuzana" last="Šinkorová">Zuzana Šinkorová</name><name sortKey="Spelda, Stanislav" sort="Spelda, Stanislav" uniqKey="Spelda S" first="Stanislav" last="Špelda">Stanislav Špelda</name><name sortKey="Svoboda, Zbyn K" sort="Svoboda, Zbyn K" uniqKey="Svoboda Z" first="Zbyn K" last="Svoboda">Zbyn K Svoboda</name><name sortKey="Tacheci, Ilja" sort="Tacheci, Ilja" uniqKey="Tacheci I" first="Ilja" last="Tachecí">Ilja Tachecí</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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