Adipose Mesenchymal Stromal Cell Function Is Not Affected by Methotrexate and Azathioprine
Identifieur interne : 000216 ( Ncbi/Curation ); précédent : 000215; suivant : 000217Adipose Mesenchymal Stromal Cell Function Is Not Affected by Methotrexate and Azathioprine
Auteurs : Pablo Manche O-Corvo ; Marcella Franquesa ; Olga De La Rosa ; Cristina Ramírez ; Leyre García-Benzaquén ; Vanessa Fernández ; Ram N Menta ; Aitor Beraza ; Wilfried Dalemans ; Martin J. Hoogduijn ; Eleuterio LombardoSource :
- BioResearch Open Access [ 2164-7844 ] ; 2013.
Abstract
Given their capacity to modulate the immune response, adipose mesenchymal stem or stromal cells (ASCs) have been used as therapeutic tools to treat chronic inflammatory and autoimmune diseases both in preclinical and clinical studies. Patients enrolled in such clinical trials are often concomitantly treated with immunomodulatory drugs such as methotrexate (MTX) or azathioprine (AZA). Therefore it is necessary to investigate the possible impact of these drugs on ASC function to learn if there are any interactions that would affect the therapeutic effects of either component and thus the clinical outcome of the trials. ASCs were cultured in the absence or presence of MTX or AZA and the effects on viability, proliferation, immunomodulatory properties, and immunogenic features were studied
Url:
DOI: 10.1089/biores.2013.0040
PubMed: 24380053
PubMed Central: 3869447
Links toward previous steps (curation, corpus...)
- to stream Pmc, to step Corpus: Pour aller vers cette notice dans l'étape Curation :000337
- to stream Pmc, to step Curation: Pour aller vers cette notice dans l'étape Curation :000337
- to stream Pmc, to step Checkpoint: Pour aller vers cette notice dans l'étape Curation :000292
- to stream Ncbi, to step Merge: Pour aller vers cette notice dans l'étape Curation :000216
Links to Exploration step
PMC:3869447Le document en format XML
<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">Adipose Mesenchymal Stromal Cell Function Is Not Affected by Methotrexate and Azathioprine</title>
<author><name sortKey="Manche O Corvo, Pablo" sort="Manche O Corvo, Pablo" uniqKey="Manche O Corvo P" first="Pablo" last="Manche O-Corvo">Pablo Manche O-Corvo</name>
<affiliation><nlm:aff id="aff1"></nlm:aff>
</affiliation>
</author>
<author><name sortKey="Franquesa, Marcella" sort="Franquesa, Marcella" uniqKey="Franquesa M" first="Marcella" last="Franquesa">Marcella Franquesa</name>
<affiliation><nlm:aff id="aff2"></nlm:aff>
</affiliation>
</author>
<author><name sortKey="De La Rosa, Olga" sort="De La Rosa, Olga" uniqKey="De La Rosa O" first="Olga" last="De La Rosa">Olga De La Rosa</name>
<affiliation><nlm:aff id="aff1"></nlm:aff>
</affiliation>
</author>
<author><name sortKey="Ramirez, Cristina" sort="Ramirez, Cristina" uniqKey="Ramirez C" first="Cristina" last="Ramírez">Cristina Ramírez</name>
<affiliation><nlm:aff id="aff1"></nlm:aff>
</affiliation>
</author>
<author><name sortKey="Garcia Benzaquen, Leyre" sort="Garcia Benzaquen, Leyre" uniqKey="Garcia Benzaquen L" first="Leyre" last="García-Benzaquén">Leyre García-Benzaquén</name>
<affiliation><nlm:aff id="aff1"></nlm:aff>
</affiliation>
</author>
<author><name sortKey="Fernandez, Vanessa" sort="Fernandez, Vanessa" uniqKey="Fernandez V" first="Vanessa" last="Fernández">Vanessa Fernández</name>
<affiliation><nlm:aff id="aff1"></nlm:aff>
</affiliation>
</author>
<author><name sortKey="Menta, Ram N" sort="Menta, Ram N" uniqKey="Menta R" first="Ram N" last="Menta">Ram N Menta</name>
<affiliation><nlm:aff id="aff1"></nlm:aff>
</affiliation>
</author>
<author><name sortKey="Beraza, Aitor" sort="Beraza, Aitor" uniqKey="Beraza A" first="Aitor" last="Beraza">Aitor Beraza</name>
<affiliation><nlm:aff id="aff1"></nlm:aff>
</affiliation>
</author>
<author><name sortKey="Dalemans, Wilfried" sort="Dalemans, Wilfried" uniqKey="Dalemans W" first="Wilfried" last="Dalemans">Wilfried Dalemans</name>
<affiliation><nlm:aff id="aff3"></nlm:aff>
</affiliation>
</author>
<author><name sortKey="Hoogduijn, Martin J" sort="Hoogduijn, Martin J" uniqKey="Hoogduijn M" first="Martin J." last="Hoogduijn">Martin J. Hoogduijn</name>
<affiliation><nlm:aff id="aff2"></nlm:aff>
</affiliation>
</author>
<author><name sortKey="Lombardo, Eleuterio" sort="Lombardo, Eleuterio" uniqKey="Lombardo E" first="Eleuterio" last="Lombardo">Eleuterio Lombardo</name>
<affiliation><nlm:aff id="aff1"></nlm:aff>
</affiliation>
</author>
</titleStmt>
<publicationStmt><idno type="wicri:source">PMC</idno>
<idno type="pmid">24380053</idno>
<idno type="pmc">3869447</idno>
<idno type="url">http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3869447</idno>
<idno type="RBID">PMC:3869447</idno>
<idno type="doi">10.1089/biores.2013.0040</idno>
<date when="2013">2013</date>
<idno type="wicri:Area/Pmc/Corpus">000337</idno>
<idno type="wicri:Area/Pmc/Curation">000337</idno>
<idno type="wicri:Area/Pmc/Checkpoint">000292</idno>
<idno type="wicri:Area/Ncbi/Merge">000216</idno>
<idno type="wicri:Area/Ncbi/Curation">000216</idno>
</publicationStmt>
<sourceDesc><biblStruct><analytic><title xml:lang="en" level="a" type="main">Adipose Mesenchymal Stromal Cell Function Is Not Affected by Methotrexate and Azathioprine</title>
<author><name sortKey="Manche O Corvo, Pablo" sort="Manche O Corvo, Pablo" uniqKey="Manche O Corvo P" first="Pablo" last="Manche O-Corvo">Pablo Manche O-Corvo</name>
<affiliation><nlm:aff id="aff1"></nlm:aff>
</affiliation>
</author>
<author><name sortKey="Franquesa, Marcella" sort="Franquesa, Marcella" uniqKey="Franquesa M" first="Marcella" last="Franquesa">Marcella Franquesa</name>
<affiliation><nlm:aff id="aff2"></nlm:aff>
</affiliation>
</author>
<author><name sortKey="De La Rosa, Olga" sort="De La Rosa, Olga" uniqKey="De La Rosa O" first="Olga" last="De La Rosa">Olga De La Rosa</name>
<affiliation><nlm:aff id="aff1"></nlm:aff>
</affiliation>
</author>
<author><name sortKey="Ramirez, Cristina" sort="Ramirez, Cristina" uniqKey="Ramirez C" first="Cristina" last="Ramírez">Cristina Ramírez</name>
<affiliation><nlm:aff id="aff1"></nlm:aff>
</affiliation>
</author>
<author><name sortKey="Garcia Benzaquen, Leyre" sort="Garcia Benzaquen, Leyre" uniqKey="Garcia Benzaquen L" first="Leyre" last="García-Benzaquén">Leyre García-Benzaquén</name>
<affiliation><nlm:aff id="aff1"></nlm:aff>
</affiliation>
</author>
<author><name sortKey="Fernandez, Vanessa" sort="Fernandez, Vanessa" uniqKey="Fernandez V" first="Vanessa" last="Fernández">Vanessa Fernández</name>
<affiliation><nlm:aff id="aff1"></nlm:aff>
</affiliation>
</author>
<author><name sortKey="Menta, Ram N" sort="Menta, Ram N" uniqKey="Menta R" first="Ram N" last="Menta">Ram N Menta</name>
<affiliation><nlm:aff id="aff1"></nlm:aff>
</affiliation>
</author>
<author><name sortKey="Beraza, Aitor" sort="Beraza, Aitor" uniqKey="Beraza A" first="Aitor" last="Beraza">Aitor Beraza</name>
<affiliation><nlm:aff id="aff1"></nlm:aff>
</affiliation>
</author>
<author><name sortKey="Dalemans, Wilfried" sort="Dalemans, Wilfried" uniqKey="Dalemans W" first="Wilfried" last="Dalemans">Wilfried Dalemans</name>
<affiliation><nlm:aff id="aff3"></nlm:aff>
</affiliation>
</author>
<author><name sortKey="Hoogduijn, Martin J" sort="Hoogduijn, Martin J" uniqKey="Hoogduijn M" first="Martin J." last="Hoogduijn">Martin J. Hoogduijn</name>
<affiliation><nlm:aff id="aff2"></nlm:aff>
</affiliation>
</author>
<author><name sortKey="Lombardo, Eleuterio" sort="Lombardo, Eleuterio" uniqKey="Lombardo E" first="Eleuterio" last="Lombardo">Eleuterio Lombardo</name>
<affiliation><nlm:aff id="aff1"></nlm:aff>
</affiliation>
</author>
</analytic>
<series><title level="j">BioResearch Open Access</title>
<idno type="ISSN">2164-7844</idno>
<idno type="eISSN">2164-7860</idno>
<imprint><date when="2013">2013</date>
</imprint>
</series>
</biblStruct>
</sourceDesc>
</fileDesc>
<profileDesc><textClass></textClass>
</profileDesc>
</teiHeader>
<front><div type="abstract" xml:lang="en"><title>Abstract</title>
<p>Given their capacity to modulate the immune response, adipose mesenchymal stem or stromal cells (ASCs) have been used as therapeutic tools to treat chronic inflammatory and autoimmune diseases both in preclinical and clinical studies. Patients enrolled in such clinical trials are often concomitantly treated with immunomodulatory drugs such as methotrexate (MTX) or azathioprine (AZA). Therefore it is necessary to investigate the possible impact of these drugs on ASC function to learn if there are any interactions that would affect the therapeutic effects of either component and thus the clinical outcome of the trials. ASCs were cultured in the absence or presence of MTX or AZA and the effects on viability, proliferation, immunomodulatory properties, and immunogenic features were studied <italic>in vitro</italic>
. The drugs did not affect the viability and proliferative capacity of ASCs. When the drugs and the ASCs were concomitantly used to inhibit lymphocyte proliferation, no synergistic or antagonizing inhibitory effects were found. MTX and AZA did not impair the capacity of ASCs to induce the generation of regulatory T cells <italic>in vitro</italic>
. These data confirm that the immunomodulating features of ASCs are fully functional after exposure to these drugs. Interestingly, whereas MTX did not affect the capacity of natural killer (NK) cells to lyse allogeneic ASCs <italic>in vitro</italic>
, AZA protected allogeneic ASCs from NK cell lysis.</p>
</div>
</front>
<back><div1 type="bibliography"><listBibl><biblStruct><analytic><author><name sortKey="Friedenstein, Aj" uniqKey="Friedenstein A">AJ Friedenstein</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Zuk, Pa" uniqKey="Zuk P">PA Zuk</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Fukuchi, Y" uniqKey="Fukuchi Y">Y Fukuchi</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Romanov, Ya" uniqKey="Romanov Y">YA Romanov</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Gronthos, S" uniqKey="Gronthos S">S Gronthos</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="De Bari, C" uniqKey="De Bari C">C De Bari</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Gonzalez, Ma" uniqKey="Gonzalez M">MA González</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Gonzalez, Ma" uniqKey="Gonzalez M">MA González</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Gonzalez Rey, E" uniqKey="Gonzalez Rey E">E González-Rey</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Gonzalez Rey, E" uniqKey="Gonzalez Rey E">E González-Rey</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Garcia Olmo, D" uniqKey="Garcia Olmo D">D Garcia-Olmo</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Duijvestein, M" uniqKey="Duijvestein M">M Duijvestein</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Ciccocioppo, R" uniqKey="Ciccocioppo R">R Ciccocioppo</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="De La Portilla, F" uniqKey="De La Portilla F">F de la Portilla</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Singer, Ng" uniqKey="Singer N">NG Singer</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Doorn, J" uniqKey="Doorn J">J Doorn</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Meisel, R" uniqKey="Meisel R">R Meisel</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Delarosa, O" uniqKey="Delarosa O">O DelaRosa</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Maccario, R" uniqKey="Maccario R">R Maccario</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Munn, Dh" uniqKey="Munn D">DH Munn</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Le Blanc, K" uniqKey="Le Blanc K">K Le Blanc</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Majumdar, Mk" uniqKey="Majumdar M">MK Majumdar</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Mcintosh, K" uniqKey="Mcintosh K">K McIntosh</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Chamberlain, G" uniqKey="Chamberlain G">G Chamberlain</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Griffin, Md" uniqKey="Griffin M">MD Griffin</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Kay, J" uniqKey="Kay J">J Kay</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Preiss, Jc" uniqKey="Preiss J">JC Preiss</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Blonski, W" uniqKey="Blonski W">W Blonski</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Cutolo, M" uniqKey="Cutolo M">M Cutolo</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Tiede, I" uniqKey="Tiede I">I Tiede</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Maltzman, Js" uniqKey="Maltzman J">JS Maltzman</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Duijvestein, M" uniqKey="Duijvestein M">M Duijvestein</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Crop, Mj" uniqKey="Crop M">MJ Crop</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Cseuz, R" uniqKey="Cseuz R">R Cseuz</name>
</author>
</analytic>
</biblStruct>
</listBibl>
</div1>
</back>
</TEI>
</record>
Pour manipuler ce document sous Unix (Dilib)
EXPLOR_STEP=$WICRI_ROOT/Wicri/Belgique/explor/OpenAccessBelV2/Data/Ncbi/Curation
HfdSelect -h $EXPLOR_STEP/biblio.hfd -nk 000216 | SxmlIndent | more
Ou
HfdSelect -h $EXPLOR_AREA/Data/Ncbi/Curation/biblio.hfd -nk 000216 | SxmlIndent | more
Pour mettre un lien sur cette page dans le réseau Wicri
{{Explor lien |wiki= Wicri/Belgique |area= OpenAccessBelV2 |flux= Ncbi |étape= Curation |type= RBID |clé= PMC:3869447 |texte= Adipose Mesenchymal Stromal Cell Function Is Not Affected by Methotrexate and Azathioprine }}
Pour générer des pages wiki
HfdIndexSelect -h $EXPLOR_AREA/Data/Ncbi/Curation/RBID.i -Sk "pubmed:24380053" \ | HfdSelect -Kh $EXPLOR_AREA/Data/Ncbi/Curation/biblio.hfd \ | NlmPubMed2Wicri -a OpenAccessBelV2
This area was generated with Dilib version V0.6.25. |