Pharmaceutical analysis by supercritical fluid chromatography : Optimization of the mobile phase composition on a 2-ethylpyridine column
Identifieur interne : 001425 ( Main/Merge ); précédent : 001424; suivant : 001426Pharmaceutical analysis by supercritical fluid chromatography : Optimization of the mobile phase composition on a 2-ethylpyridine column
Auteurs : Claudio Brunelli [Belgique] ; YINING ZHAO [États-Unis] ; Melissa-Hanna Brown [Royaume-Uni] ; Pat Sandra [Belgique]Source :
- Journal of separation science [ 1615-9306 ] ; 2008.
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- Pascal (Inist)
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- topic : Médicament.
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Abstract
The separation of neutral, acidic, and basic pharmaceuticals with diverse physicochemical properties by packed column supercritical fluid chromatography (pSFC) on a 2-ethylpyridine column (25 cm x 4.6 mm id, 3 μm particles) is presented. The optimization strategy involved separations at 100% methanol (MeOH) and at 50% MeOH/50% ACN while keeping the peak symmetry additives formic acid (FA) and isopropylamine (IPA) at constant levels of 0.25% v/v. By plotting the adjusted retention times as a function of the MeOH/ACN ratio, an optimal modifier ratio composition of 65% MeOH/35% ACN was found. The total set of 26 neutral, acidic, and basic pharmaceuticals was analyzed and the optimal composition experimentally verified. This mobile phase composition is currently used in pharmaceutical method development and open-access generic screening environments.
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type="state">Connecticut</region></placeName></affiliation></author><author><name sortKey="Brown, Melissa Hanna" sort="Brown, Melissa Hanna" uniqKey="Brown M" first="Melissa-Hanna" last="Brown">Melissa-Hanna Brown</name><affiliation wicri:level="1"><inist:fA14 i1="03"><s1>Pfizer Global R&D, Analytical R&D</s1><s2>Sandwich, Kent</s2><s3>GBR</s3><sZ>3 aut.</sZ></inist:fA14><country>Royaume-Uni</country><wicri:noRegion>Sandwich, Kent</wicri:noRegion></affiliation></author><author><name sortKey="Sandra, Pat" sort="Sandra, Pat" uniqKey="Sandra P" first="Pat" last="Sandra">Pat Sandra</name><affiliation wicri:level="1"><inist:fA14 i1="01"><s1>Pfizer Analytical Research Centre, Ghent University</s1><s2>Ghent</s2><s3>BEL</s3><sZ>1 aut.</sZ><sZ>4 aut.</sZ></inist:fA14><country>Belgique</country><wicri:noRegion>Ghent</wicri:noRegion></affiliation></author></titleStmt><publicationStmt><idno type="wicri:source">INIST</idno><idno type="inist">08-0393504</idno><date when="2008">2008</date><idno 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aut.</sZ></inist:fA14><country>Belgique</country><wicri:noRegion>Ghent</wicri:noRegion></affiliation></author><author><name sortKey="Yining Zhao" sort="Yining Zhao" uniqKey="Yining Zhao" last="Yining Zhao">YINING ZHAO</name><affiliation wicri:level="2"><inist:fA14 i1="02"><s1>Analytical R&D, Pfizer Global R&D</s1><s2>Groton, CT</s2><s3>USA</s3><sZ>2 aut.</sZ></inist:fA14><country>États-Unis</country><placeName><region type="state">Connecticut</region></placeName></affiliation></author><author><name sortKey="Brown, Melissa Hanna" sort="Brown, Melissa Hanna" uniqKey="Brown M" first="Melissa-Hanna" last="Brown">Melissa-Hanna Brown</name><affiliation wicri:level="1"><inist:fA14 i1="03"><s1>Pfizer Global R&D, Analytical R&D</s1><s2>Sandwich, Kent</s2><s3>GBR</s3><sZ>3 aut.</sZ></inist:fA14><country>Royaume-Uni</country><wicri:noRegion>Sandwich, Kent</wicri:noRegion></affiliation></author><author><name sortKey="Sandra, Pat" sort="Sandra, Pat" uniqKey="Sandra P" first="Pat" last="Sandra">Pat Sandra</name><affiliation wicri:level="1"><inist:fA14 i1="01"><s1>Pfizer Analytical Research Centre, Ghent University</s1><s2>Ghent</s2><s3>BEL</s3><sZ>1 aut.</sZ><sZ>4 aut.</sZ></inist:fA14><country>Belgique</country><wicri:noRegion>Ghent</wicri:noRegion></affiliation></author></analytic><series><title level="j" type="main">Journal of separation science</title><title level="j" type="abbreviated">J. sep. sci. : (print)</title><idno type="ISSN">1615-9306</idno><imprint><date when="2008">2008</date></imprint></series></biblStruct></sourceDesc><seriesStmt><title level="j" type="main">Journal of separation science</title><title level="j" type="abbreviated">J. sep. sci. : (print)</title><idno type="ISSN">1615-9306</idno></seriesStmt></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Chemical analysis</term><term>Drug</term><term>Mobile phase</term><term>Optimization</term><term>Phase composition</term><term>Stationary phase</term><term>Supercritical fluid chromatography</term></keywords><keywords scheme="Pascal" xml:lang="fr"><term>Analyse chimique</term><term>Médicament</term><term>Chromatographie SFC</term><term>Optimisation</term><term>Phase stationnaire</term><term>Phase mobile</term><term>Composition phase</term><term>Composition phase mobile</term><term>Pyridine(2-éthyl)</term></keywords><keywords scheme="Wicri" type="topic" xml:lang="fr"><term>Médicament</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">The separation of neutral, acidic, and basic pharmaceuticals with diverse physicochemical properties by packed column supercritical fluid chromatography (pSFC) on a 2-ethylpyridine column (25 cm x 4.6 mm id, 3 μm particles) is presented. The optimization strategy involved separations at 100% methanol (MeOH) and at 50% MeOH/50% ACN while keeping the peak symmetry additives formic acid (FA) and isopropylamine (IPA) at constant levels of 0.25% v/v. By plotting the adjusted retention times as a function of the MeOH/ACN ratio, an optimal modifier ratio composition of 65% MeOH/35% ACN was found. The total set of 26 neutral, acidic, and basic pharmaceuticals was analyzed and the optimal composition experimentally verified. This mobile phase composition is currently used in pharmaceutical method development and open-access generic screening environments.</div></front></TEI><affiliations><list><country><li>Belgique</li><li>Royaume-Uni</li><li>États-Unis</li></country><region><li>Connecticut</li></region></list><tree><country name="Belgique"><noRegion><name sortKey="Brunelli, Claudio" sort="Brunelli, Claudio" uniqKey="Brunelli C" first="Claudio" last="Brunelli">Claudio Brunelli</name></noRegion><name sortKey="Sandra, Pat" sort="Sandra, Pat" uniqKey="Sandra P" first="Pat" last="Sandra">Pat Sandra</name></country><country name="États-Unis"><region name="Connecticut"><name sortKey="Yining Zhao" sort="Yining Zhao" uniqKey="Yining Zhao" last="Yining Zhao">YINING ZHAO</name></region></country><country name="Royaume-Uni"><noRegion><name sortKey="Brown, Melissa Hanna" sort="Brown, Melissa Hanna" uniqKey="Brown M" first="Melissa-Hanna" last="Brown">Melissa-Hanna Brown</name></noRegion></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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