Renal Anemia Induced by Chronic Ingestion of Depleted Uranium in Rats
Identifieur interne : 001297 ( Main/Curation ); précédent : 001296; suivant : 001298Renal Anemia Induced by Chronic Ingestion of Depleted Uranium in Rats
Auteurs : Hana Berradi [France] ; Jean-Marc Bertho [France] ; Nicolas Dudoignon [France] ; Andr Mazur [France] ; Line Grandcolas [France] ; Cdric Baudelin [France] ; Stphane Grison [France] ; Philippe Voisin [France] ; Patrick Gourmelon [France] ; Isabelle Dublineau [France]Source :
- Toxicological Sciences [ 1096-6080 ] ; 2008-03-28.
Abstract
Kidney disease is a frequent consequence of heavy metal exposure and renal anemia occurs secondarily to the progression of kidney deterioration into chronic disease. In contrast, little is known about effects on kidney of chronic exposure to low levels of depleted uranium (DU). Study was performed with rats exposed to DU at 40 mg/l by chronic ingestion during 9 months. In the present work, a 20% reduction in red blood cell (RBC) count was observed after DU exposure. Hence, three hypotheses were tested to determinate origin of RBC loss: (1) reduced erythropoiesis, (2) increased RBC degradation, and/or (3) kidney dysfunction. Erythropoiesis was not reduced after exposure to DU as revealed by erythroid progenitors, blood Flt3 ligand and erythropoietin (EPO) blood and kidney levels. Concerning messenger RNA (mRNA) and protein levels of spleen iron recycling markers from RBC degradation (DMT1 [divalent metal transporter 1], iron regulated protein 1, HO1, HO2 [heme oxygenase 1 and 2], cluster of differentiation 36), increase in HO2 and DMT1 mRNA level was induced after chronic exposure to DU. Kidneys of DU-contaminated rats had more frequently high grade tubulo-interstitial and glomerular lesions, accumulated iron more frequently and presented more apoptotic cells. In addition, chronic exposure to DU induced increased gene expression of ceruloplasmin (12), of DMT1 (2.5), and decreased mRNA levels of erythropoietin receptor (0.2). Increased mRNA level of DMT1 was associated to decreased protein level (0.25). To conclude, a chronic ingestion of DU leads mainly to kidney deterioration that is probably responsible for RBC count decrease in rats. Spleen erythropoiesis and molecules involved in erythrocyte degradation were also modified by chronic DU exposure.
Url:
DOI: 10.1093/toxsci/kfn052
Links toward previous steps (curation, corpus...)
- to stream Istex, to step Corpus: Pour aller vers cette notice dans l'étape Curation :001252
- to stream Istex, to step Curation: Pour aller vers cette notice dans l'étape Curation :001228
- to stream Istex, to step Checkpoint: Pour aller vers cette notice dans l'étape Curation :000D83
- to stream Main, to step Merge: Pour aller vers cette notice dans l'étape Curation :001300
Links to Exploration step
ISTEX:AA97878069EF21DC10968DDAFCEC6D48E7371109Le document en format XML
<record><TEI wicri:istexFullTextTei="biblStruct"><teiHeader><fileDesc><titleStmt><title>Renal Anemia Induced by Chronic Ingestion of Depleted Uranium in Rats</title>
<author><name sortKey="Berradi, Hana" sort="Berradi, Hana" uniqKey="Berradi H" first="Hana" last="Berradi">Hana Berradi</name>
</author>
<author><name sortKey="Bertho, Jean Marc" sort="Bertho, Jean Marc" uniqKey="Bertho J" first="Jean-Marc" last="Bertho">Jean-Marc Bertho</name>
</author>
<author><name sortKey="Dudoignon, Nicolas" sort="Dudoignon, Nicolas" uniqKey="Dudoignon N" first="Nicolas" last="Dudoignon">Nicolas Dudoignon</name>
</author>
<author><name sortKey="Mazur, Andr" sort="Mazur, Andr" uniqKey="Mazur A" first="Andr" last="Mazur">Andr Mazur</name>
</author>
<author><name sortKey="Grandcolas, Line" sort="Grandcolas, Line" uniqKey="Grandcolas L" first="Line" last="Grandcolas">Line Grandcolas</name>
</author>
<author><name sortKey="Baudelin, Cdric" sort="Baudelin, Cdric" uniqKey="Baudelin C" first="Cdric" last="Baudelin">Cdric Baudelin</name>
</author>
<author><name sortKey="Grison, Stphane" sort="Grison, Stphane" uniqKey="Grison S" first="Stphane" last="Grison">Stphane Grison</name>
</author>
<author><name sortKey="Voisin, Philippe" sort="Voisin, Philippe" uniqKey="Voisin P" first="Philippe" last="Voisin">Philippe Voisin</name>
</author>
<author><name sortKey="Gourmelon, Patrick" sort="Gourmelon, Patrick" uniqKey="Gourmelon P" first="Patrick" last="Gourmelon">Patrick Gourmelon</name>
</author>
<author><name sortKey="Dublineau, Isabelle" sort="Dublineau, Isabelle" uniqKey="Dublineau I" first="Isabelle" last="Dublineau">Isabelle Dublineau</name>
</author>
</titleStmt>
<publicationStmt><idno type="wicri:source">ISTEX</idno>
<idno type="RBID">ISTEX:AA97878069EF21DC10968DDAFCEC6D48E7371109</idno>
<date when="2008" year="2008">2008</date>
<idno type="doi">10.1093/toxsci/kfn052</idno>
<idno type="url">https://api.istex.fr/document/AA97878069EF21DC10968DDAFCEC6D48E7371109/fulltext/pdf</idno>
<idno type="wicri:Area/Istex/Corpus">001252</idno>
<idno type="wicri:Area/Istex/Curation">001228</idno>
<idno type="wicri:Area/Istex/Checkpoint">000D83</idno>
<idno type="wicri:doubleKey">1096-6080:2008:Berradi H:renal:anemia:induced</idno>
<idno type="wicri:Area/Main/Merge">001300</idno>
<idno type="wicri:Area/Main/Curation">001297</idno>
</publicationStmt>
<sourceDesc><biblStruct><analytic><title level="a">Renal Anemia Induced by Chronic Ingestion of Depleted Uranium in Rats</title>
<author><name sortKey="Berradi, Hana" sort="Berradi, Hana" uniqKey="Berradi H" first="Hana" last="Berradi">Hana Berradi</name>
<affiliation wicri:level="3"><country xml:lang="fr">France</country>
<wicri:regionArea>Institut de Radioprotection et de Sret Nuclaire, Direction de la RadioProtection de l'Homme, Service de Radiobiologie et d'Epidmiologie, F-92262 Fontenay-aux-Roses Cedex</wicri:regionArea>
<placeName><region type="region" nuts="2">Île-de-France</region>
<settlement type="city">Fontenay-aux-Roses</settlement>
</placeName>
</affiliation>
</author>
<author><name sortKey="Bertho, Jean Marc" sort="Bertho, Jean Marc" uniqKey="Bertho J" first="Jean-Marc" last="Bertho">Jean-Marc Bertho</name>
<affiliation wicri:level="3"><country xml:lang="fr">France</country>
<wicri:regionArea>Institut de Radioprotection et de Sret Nuclaire, Direction de la RadioProtection de l'Homme, Service de Radiobiologie et d'Epidmiologie, F-92262 Fontenay-aux-Roses Cedex</wicri:regionArea>
<placeName><region type="region" nuts="2">Île-de-France</region>
<settlement type="city">Fontenay-aux-Roses</settlement>
</placeName>
</affiliation>
</author>
<author><name sortKey="Dudoignon, Nicolas" sort="Dudoignon, Nicolas" uniqKey="Dudoignon N" first="Nicolas" last="Dudoignon">Nicolas Dudoignon</name>
<affiliation wicri:level="3"><country xml:lang="fr">France</country>
<wicri:regionArea>Institut de Radioprotection et de Sret Nuclaire, Direction de la RadioProtection de l'Homme, Service de Radiobiologie et d'Epidmiologie, F-92262 Fontenay-aux-Roses Cedex</wicri:regionArea>
<placeName><region type="region" nuts="2">Île-de-France</region>
<settlement type="city">Fontenay-aux-Roses</settlement>
</placeName>
</affiliation>
</author>
<author><name sortKey="Mazur, Andr" sort="Mazur, Andr" uniqKey="Mazur A" first="Andr" last="Mazur">Andr Mazur</name>
<affiliation wicri:level="3"><country xml:lang="fr">France</country>
<wicri:regionArea>Unit des Maladies Mtaboliques et Micronutriments, Institut National de la Recherche Agronomique, Centre de Clermont-Ferrand/Theix, F63122 Saint-Genes Champanelle</wicri:regionArea>
<placeName><region type="region" nuts="2">Auvergne-Rhône-Alpes</region>
<region type="old region" nuts="2">Auvergne (région administrative)</region>
<settlement type="city">Saint-Genes Champanelle</settlement>
</placeName>
</affiliation>
</author>
<author><name sortKey="Grandcolas, Line" sort="Grandcolas, Line" uniqKey="Grandcolas L" first="Line" last="Grandcolas">Line Grandcolas</name>
<affiliation wicri:level="3"><country xml:lang="fr">France</country>
<wicri:regionArea>Institut de Radioprotection et de Sret Nuclaire, Direction de la RadioProtection de l'Homme, Service de Radiobiologie et d'Epidmiologie, F-92262 Fontenay-aux-Roses Cedex</wicri:regionArea>
<placeName><region type="region" nuts="2">Île-de-France</region>
<settlement type="city">Fontenay-aux-Roses</settlement>
</placeName>
</affiliation>
</author>
<author><name sortKey="Baudelin, Cdric" sort="Baudelin, Cdric" uniqKey="Baudelin C" first="Cdric" last="Baudelin">Cdric Baudelin</name>
<affiliation wicri:level="3"><country xml:lang="fr">France</country>
<wicri:regionArea>Institut de Radioprotection et de Sret Nuclaire, Direction de la RadioProtection de l'Homme, Service de Radiobiologie et d'Epidmiologie, F-92262 Fontenay-aux-Roses Cedex</wicri:regionArea>
<placeName><region type="region" nuts="2">Île-de-France</region>
<settlement type="city">Fontenay-aux-Roses</settlement>
</placeName>
</affiliation>
</author>
<author><name sortKey="Grison, Stphane" sort="Grison, Stphane" uniqKey="Grison S" first="Stphane" last="Grison">Stphane Grison</name>
<affiliation wicri:level="3"><country xml:lang="fr">France</country>
<wicri:regionArea>Institut de Radioprotection et de Sret Nuclaire, Direction de la RadioProtection de l'Homme, Service de Radiobiologie et d'Epidmiologie, F-92262 Fontenay-aux-Roses Cedex</wicri:regionArea>
<placeName><region type="region" nuts="2">Île-de-France</region>
<settlement type="city">Fontenay-aux-Roses</settlement>
</placeName>
</affiliation>
</author>
<author><name sortKey="Voisin, Philippe" sort="Voisin, Philippe" uniqKey="Voisin P" first="Philippe" last="Voisin">Philippe Voisin</name>
<affiliation wicri:level="3"><country xml:lang="fr">France</country>
<wicri:regionArea>Institut de Radioprotection et de Sret Nuclaire, Direction de la RadioProtection de l'Homme, Service de Radiobiologie et d'Epidmiologie, F-92262 Fontenay-aux-Roses Cedex</wicri:regionArea>
<placeName><region type="region" nuts="2">Île-de-France</region>
<settlement type="city">Fontenay-aux-Roses</settlement>
</placeName>
</affiliation>
</author>
<author><name sortKey="Gourmelon, Patrick" sort="Gourmelon, Patrick" uniqKey="Gourmelon P" first="Patrick" last="Gourmelon">Patrick Gourmelon</name>
<affiliation wicri:level="3"><country xml:lang="fr">France</country>
<wicri:regionArea>Institut de Radioprotection et de Sret Nuclaire, Direction de la RadioProtection de l'Homme, Service de Radiobiologie et d'Epidmiologie, F-92262 Fontenay-aux-Roses Cedex</wicri:regionArea>
<placeName><region type="region" nuts="2">Île-de-France</region>
<settlement type="city">Fontenay-aux-Roses</settlement>
</placeName>
</affiliation>
</author>
<author><name sortKey="Dublineau, Isabelle" sort="Dublineau, Isabelle" uniqKey="Dublineau I" first="Isabelle" last="Dublineau">Isabelle Dublineau</name>
<affiliation wicri:level="3"><country xml:lang="fr">France</country>
<wicri:regionArea>Institut de Radioprotection et de Sret Nuclaire, Direction de la RadioProtection de l'Homme, Service de Radiobiologie et d'Epidmiologie, F-92262 Fontenay-aux-Roses Cedex</wicri:regionArea>
<placeName><region type="region" nuts="2">Île-de-France</region>
<settlement type="city">Fontenay-aux-Roses</settlement>
</placeName>
</affiliation>
<affiliation wicri:level="1"><country wicri:rule="url">France</country>
</affiliation>
</author>
</analytic>
<monogr></monogr>
<series><title level="j">Toxicological Sciences</title>
<idno type="ISSN">1096-6080</idno>
<idno type="eISSN">1096-0929</idno>
<imprint><publisher>Oxford University Press</publisher>
<date type="published" when="2008-03-28">2008-03-28</date>
<biblScope unit="volume">103</biblScope>
<biblScope unit="issue">2</biblScope>
<biblScope unit="page" from="397">397</biblScope>
<biblScope unit="page" to="408">408</biblScope>
</imprint>
<idno type="ISSN">1096-6080</idno>
</series>
<idno type="istex">AA97878069EF21DC10968DDAFCEC6D48E7371109</idno>
<idno type="DOI">10.1093/toxsci/kfn052</idno>
</biblStruct>
</sourceDesc>
<seriesStmt><idno type="ISSN">1096-6080</idno>
</seriesStmt>
</fileDesc>
<profileDesc><textClass></textClass>
<langUsage><language ident="en">en</language>
</langUsage>
</profileDesc>
</teiHeader>
<front><div type="abstract">Kidney disease is a frequent consequence of heavy metal exposure and renal anemia occurs secondarily to the progression of kidney deterioration into chronic disease. In contrast, little is known about effects on kidney of chronic exposure to low levels of depleted uranium (DU). Study was performed with rats exposed to DU at 40 mg/l by chronic ingestion during 9 months. In the present work, a 20% reduction in red blood cell (RBC) count was observed after DU exposure. Hence, three hypotheses were tested to determinate origin of RBC loss: (1) reduced erythropoiesis, (2) increased RBC degradation, and/or (3) kidney dysfunction. Erythropoiesis was not reduced after exposure to DU as revealed by erythroid progenitors, blood Flt3 ligand and erythropoietin (EPO) blood and kidney levels. Concerning messenger RNA (mRNA) and protein levels of spleen iron recycling markers from RBC degradation (DMT1 [divalent metal transporter 1], iron regulated protein 1, HO1, HO2 [heme oxygenase 1 and 2], cluster of differentiation 36), increase in HO2 and DMT1 mRNA level was induced after chronic exposure to DU. Kidneys of DU-contaminated rats had more frequently high grade tubulo-interstitial and glomerular lesions, accumulated iron more frequently and presented more apoptotic cells. In addition, chronic exposure to DU induced increased gene expression of ceruloplasmin (12), of DMT1 (2.5), and decreased mRNA levels of erythropoietin receptor (0.2). Increased mRNA level of DMT1 was associated to decreased protein level (0.25). To conclude, a chronic ingestion of DU leads mainly to kidney deterioration that is probably responsible for RBC count decrease in rats. Spleen erythropoiesis and molecules involved in erythrocyte degradation were also modified by chronic DU exposure.</div>
</front>
</TEI>
</record>
Pour manipuler ce document sous Unix (Dilib)
EXPLOR_STEP=$WICRI_ROOT/Wicri/Belgique/explor/OpenAccessBelV2/Data/Main/Curation
HfdSelect -h $EXPLOR_STEP/biblio.hfd -nk 001297 | SxmlIndent | more
Ou
HfdSelect -h $EXPLOR_AREA/Data/Main/Curation/biblio.hfd -nk 001297 | SxmlIndent | more
Pour mettre un lien sur cette page dans le réseau Wicri
{{Explor lien |wiki= Wicri/Belgique |area= OpenAccessBelV2 |flux= Main |étape= Curation |type= RBID |clé= ISTEX:AA97878069EF21DC10968DDAFCEC6D48E7371109 |texte= Renal Anemia Induced by Chronic Ingestion of Depleted Uranium in Rats }}
![]() | This area was generated with Dilib version V0.6.25. | ![]() |