GCN2-dependent phosphorylation of eukaryotic translation initiation factor-2 in Arabidopsis
Identifieur interne : 001515 ( Istex/Corpus ); précédent : 001514; suivant : 001516GCN2-dependent phosphorylation of eukaryotic translation initiation factor-2 in Arabidopsis
Auteurs : Yuhua Zhang ; Yifei Wang ; Kostya Kanyuka ; Martin A. J. Parry ; Stephen J. Powers ; Nigel G. HalfordSource :
- Journal of Experimental Botany [ 0022-0957 ] ; 2008-07-04.
Abstract
The yeast regulatory protein kinase, general control non-derepressible-2 (GCN2) plays a key role in general amino acid control. GCN2 phosphorylates the subunit of the trimeric eukaryotic translation initiation factor-2 (eIF2), bringing about a decrease in the general rate of protein synthesis but an increase in the synthesis of GCN4, a transcription factor that promotes the expression of genes encoding enzymes for amino acid biosynthesis. The present study concerned the phosphorylation of Arabidopsis eIF2 (AteIF2) by the Arabidopsis homologue of GCN2, AtGCN2, and the role of AtGCN2 in regulating genes encoding enzymes of amino acid biosynthesis and responding to virus infection. A null mutant for AtGCN2 called GT8359 was obtained and western analysis confirmed that it lacked AtGCN2 protein. GT8359 was more sensitive than wild-type Arabidopsis to herbicides that affect amino acid biosynthesis. Phosphorylation of AteIF2 occurred in response to herbicide treatment but only in wild-type Arabidopsis, not GT8359, showing it to be AtGCN2-dependent. Expression analysis of genes encoding key enzymes for amino acid biosynthesis and nitrate assimilation revealed little effect of loss of AtGCN2 function in GT8359 except that expression of a nitrate reductase gene, NIA1, was decreased. Analysis of wild-type and GT8359 plants infected with Turnip yellow mosaic virus or Turnip crinkle virus showed that AteIF2 was not phosphorylated.
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DOI: 10.1093/jxb/ern169
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J." last="Parry">Martin A. J. Parry</name><affiliation><mods:affiliation>Centre for Crop Genetic Improvement, Plant Sciences Department, Rothamsted Research, Harpenden, Hertfordshire AL5 2JQ, UK</mods:affiliation></affiliation></author><author><name sortKey="Powers, Stephen J" sort="Powers, Stephen J" uniqKey="Powers S" first="Stephen J." last="Powers">Stephen J. Powers</name><affiliation><mods:affiliation>Centre for Mathematical and Computational Biology, Biomathematics and Bioinformatics Department, Rothamsted Research, Harpenden, Hertfordshire AL5 2JQ, UK</mods:affiliation></affiliation></author><author><name sortKey="Halford, Nigel G" sort="Halford, Nigel G" uniqKey="Halford N" first="Nigel G." last="Halford">Nigel G. Halford</name><affiliation><mods:affiliation>Centre for Crop Genetic Improvement, Plant Sciences Department, Rothamsted Research, Harpenden, Hertfordshire AL5 2JQ, UK</mods:affiliation></affiliation><affiliation><mods:affiliation>E-mail: nigel.halford@bbsrc.ac.uk</mods:affiliation></affiliation></author></titleStmt><publicationStmt><idno type="wicri:source">ISTEX</idno><idno type="RBID">ISTEX:0F0F4A2A2EB5C1448AD78F811381212C0FF6D581</idno><date when="2008" year="2008">2008</date><idno type="doi">10.1093/jxb/ern169</idno><idno type="url">https://api.istex.fr/document/0F0F4A2A2EB5C1448AD78F811381212C0FF6D581/fulltext/pdf</idno><idno type="wicri:Area/Istex/Corpus">001515</idno></publicationStmt><sourceDesc><biblStruct><analytic><title level="a">GCN2-dependent phosphorylation of eukaryotic translation initiation factor-2 in Arabidopsis</title><author><name sortKey="Zhang, Yuhua" sort="Zhang, Yuhua" uniqKey="Zhang Y" first="Yuhua" last="Zhang">Yuhua Zhang</name><affiliation><mods:affiliation>Centre for Crop Genetic Improvement, Plant Sciences Department, Rothamsted Research, Harpenden, Hertfordshire AL5 2JQ, UK</mods:affiliation></affiliation></author><author><name sortKey="Wang, Yifei" sort="Wang, Yifei" uniqKey="Wang Y" first="Yifei" last="Wang">Yifei Wang</name><affiliation><mods:affiliation>Centre for Crop Genetic Improvement, Plant Sciences Department, Rothamsted Research, Harpenden, Hertfordshire AL5 2JQ, UK</mods:affiliation></affiliation></author><author><name sortKey="Kanyuka, Kostya" sort="Kanyuka, Kostya" uniqKey="Kanyuka K" first="Kostya" last="Kanyuka">Kostya Kanyuka</name><affiliation><mods:affiliation>Centre for Sustainable Pest and Disease Management, Plant Pathology and Microbiology Department, Rothamsted Research, Harpenden, Hertfordshire AL5 2JQ, UK</mods:affiliation></affiliation></author><author><name sortKey="Parry, Martin A J" sort="Parry, Martin A J" uniqKey="Parry M" first="Martin A. 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Halford</name><affiliation><mods:affiliation>Centre for Crop Genetic Improvement, Plant Sciences Department, Rothamsted Research, Harpenden, Hertfordshire AL5 2JQ, UK</mods:affiliation></affiliation><affiliation><mods:affiliation>E-mail: nigel.halford@bbsrc.ac.uk</mods:affiliation></affiliation></author></analytic><monogr></monogr><series><title level="j">Journal of Experimental Botany</title><idno type="ISSN">0022-0957</idno><idno type="eISSN">1460-2431</idno><imprint><publisher>Oxford University Press</publisher><date type="published" when="2008-07-04">2008-07-04</date><biblScope unit="volume">59</biblScope><biblScope unit="issue">11</biblScope><biblScope unit="page" from="3131">3131</biblScope><biblScope unit="page" to="3141">3141</biblScope></imprint><idno type="ISSN">0022-0957</idno></series><idno type="istex">0F0F4A2A2EB5C1448AD78F811381212C0FF6D581</idno><idno type="DOI">10.1093/jxb/ern169</idno></biblStruct></sourceDesc><seriesStmt><idno type="ISSN">0022-0957</idno></seriesStmt></fileDesc><profileDesc><textClass></textClass><langUsage><language ident="en">en</language></langUsage></profileDesc></teiHeader><front><div type="abstract">The yeast regulatory protein kinase, general control non-derepressible-2 (GCN2) plays a key role in general amino acid control. GCN2 phosphorylates the subunit of the trimeric eukaryotic translation initiation factor-2 (eIF2), bringing about a decrease in the general rate of protein synthesis but an increase in the synthesis of GCN4, a transcription factor that promotes the expression of genes encoding enzymes for amino acid biosynthesis. The present study concerned the phosphorylation of Arabidopsis eIF2 (AteIF2) by the Arabidopsis homologue of GCN2, AtGCN2, and the role of AtGCN2 in regulating genes encoding enzymes of amino acid biosynthesis and responding to virus infection. A null mutant for AtGCN2 called GT8359 was obtained and western analysis confirmed that it lacked AtGCN2 protein. GT8359 was more sensitive than wild-type Arabidopsis to herbicides that affect amino acid biosynthesis. Phosphorylation of AteIF2 occurred in response to herbicide treatment but only in wild-type Arabidopsis, not GT8359, showing it to be AtGCN2-dependent. Expression analysis of genes encoding key enzymes for amino acid biosynthesis and nitrate assimilation revealed little effect of loss of AtGCN2 function in GT8359 except that expression of a nitrate reductase gene, NIA1, was decreased. Analysis of wild-type and GT8359 plants infected with Turnip yellow mosaic virus or Turnip crinkle virus showed that AteIF2 was not phosphorylated.</div></front></TEI><istex><corpusName>oup</corpusName><author><json:item><name>Yuhua Zhang</name><affiliations><json:string>Centre for Crop Genetic Improvement, Plant Sciences Department, Rothamsted Research, Harpenden, Hertfordshire AL5 2JQ, UK</json:string></affiliations></json:item><json:item><name>Yifei Wang</name><affiliations><json:string>Centre for Crop Genetic Improvement, Plant Sciences Department, Rothamsted Research, Harpenden, Hertfordshire AL5 2JQ, UK</json:string></affiliations></json:item><json:item><name>Kostya Kanyuka</name><affiliations><json:string>Centre for Sustainable Pest and Disease Management, Plant Pathology and Microbiology Department, Rothamsted Research, Harpenden, Hertfordshire AL5 2JQ, UK</json:string></affiliations></json:item><json:item><name>Martin A. J. 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GCN2 phosphorylates the subunit of the trimeric eukaryotic translation initiation factor-2 (eIF2), bringing about a decrease in the general rate of protein synthesis but an increase in the synthesis of GCN4, a transcription factor that promotes the expression of genes encoding enzymes for amino acid biosynthesis. The present study concerned the phosphorylation of Arabidopsis eIF2 (AteIF2) by the Arabidopsis homologue of GCN2, AtGCN2, and the role of AtGCN2 in regulating genes encoding enzymes of amino acid biosynthesis and responding to virus infection. A null mutant for AtGCN2 called GT8359 was obtained and western analysis confirmed that it lacked AtGCN2 protein. GT8359 was more sensitive than wild-type Arabidopsis to herbicides that affect amino acid biosynthesis. Phosphorylation of AteIF2 occurred in response to herbicide treatment but only in wild-type Arabidopsis, not GT8359, showing it to be AtGCN2-dependent. Expression analysis of genes encoding key enzymes for amino acid biosynthesis and nitrate assimilation revealed little effect of loss of AtGCN2 function in GT8359 except that expression of a nitrate reductase gene, NIA1, was decreased. 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J.</forename><surname>Parry</surname></persName><affiliation>Centre for Crop Genetic Improvement, Plant Sciences Department, Rothamsted Research, Harpenden, Hertfordshire AL5 2JQ, UK</affiliation></author><author xml:id="author-5"><persName><forename type="first">Stephen J.</forename><surname>Powers</surname></persName><affiliation>Centre for Mathematical and Computational Biology, Biomathematics and Bioinformatics Department, Rothamsted Research, Harpenden, Hertfordshire AL5 2JQ, UK</affiliation></author><author xml:id="author-6" corresp="yes"><persName><forename type="first">Nigel G.</forename><surname>Halford</surname></persName><email>nigel.halford@bbsrc.ac.uk</email><affiliation>Centre for Crop Genetic Improvement, Plant Sciences Department, Rothamsted Research, Harpenden, Hertfordshire AL5 2JQ, UK</affiliation></author></analytic><monogr><title level="j">Journal of Experimental Botany</title><idno type="pISSN">0022-0957</idno><idno type="eISSN">1460-2431</idno><imprint><publisher>Oxford University Press</publisher><date type="published" when="2008-07-04"></date><biblScope unit="volume">59</biblScope><biblScope unit="issue">11</biblScope><biblScope unit="page" from="3131">3131</biblScope><biblScope unit="page" to="3141">3141</biblScope></imprint></monogr><idno type="istex">0F0F4A2A2EB5C1448AD78F811381212C0FF6D581</idno><idno type="DOI">10.1093/jxb/ern169</idno></biblStruct></sourceDesc></fileDesc><profileDesc><creation><date>2008-07-04</date></creation><langUsage><language ident="en">en</language></langUsage><abstract><p>The yeast regulatory protein kinase, general control non-derepressible-2 (GCN2) plays a key role in general amino acid control. GCN2 phosphorylates the subunit of the trimeric eukaryotic translation initiation factor-2 (eIF2), bringing about a decrease in the general rate of protein synthesis but an increase in the synthesis of GCN4, a transcription factor that promotes the expression of genes encoding enzymes for amino acid biosynthesis. The present study concerned the phosphorylation of Arabidopsis eIF2 (AteIF2) by the Arabidopsis homologue of GCN2, AtGCN2, and the role of AtGCN2 in regulating genes encoding enzymes of amino acid biosynthesis and responding to virus infection. A null mutant for AtGCN2 called GT8359 was obtained and western analysis confirmed that it lacked AtGCN2 protein. GT8359 was more sensitive than wild-type Arabidopsis to herbicides that affect amino acid biosynthesis. Phosphorylation of AteIF2 occurred in response to herbicide treatment but only in wild-type Arabidopsis, not GT8359, showing it to be AtGCN2-dependent. Expression analysis of genes encoding key enzymes for amino acid biosynthesis and nitrate assimilation revealed little effect of loss of AtGCN2 function in GT8359 except that expression of a nitrate reductase gene, NIA1, was decreased. Analysis of wild-type and GT8359 plants infected with Turnip yellow mosaic virus or Turnip crinkle virus showed that AteIF2 was not phosphorylated.</p></abstract><textClass><keywords scheme="keyword"><list><item><term>Research Papers</term></item></list></keywords></textClass><textClass><keywords scheme="keyword"><list><head>keywords</head><item><term>Amino acid signalling</term></item><item><term>general control non-derepressible</term></item><item><term>metabolic regulation</term></item><item><term>phosphorylation</term></item><item><term>protein kinase</term></item><item><term>virus infection</term></item></list></keywords></textClass></profileDesc><revisionDesc><change when="2008-07-04">Created</change><change when="2008-07-04">Published</change><change xml:id="refBibs-istex" who="#ISTEX-API" when="2016-10-14">References added</change></revisionDesc></teiHeader></istex:fulltextTEI><json:item><original>false</original><mimetype>text/plain</mimetype><extension>txt</extension><uri>https://api.istex.fr/document/0F0F4A2A2EB5C1448AD78F811381212C0FF6D581/fulltext/txt</uri></json:item></fulltext><metadata><istex:metadataXml wicri:clean="corpus oup" wicri:toSee="no header"><istex:docType PUBLIC="-//NLM//DTD Journal Publishing DTD v2.3 20070202//EN" URI="journalpublishing.dtd" name="istex:docType"></istex:docType><istex:document><article article-type="research-article"><front><journal-meta><journal-id journal-id-type="hwp">jexbot</journal-id><journal-id journal-id-type="publisher-id">exbotj</journal-id><journal-title>Journal of Experimental Botany</journal-title><issn pub-type="epub">1460-2431</issn><issn pub-type="ppub">0022-0957</issn><publisher><publisher-name>Oxford University Press</publisher-name></publisher></journal-meta><article-meta><article-id pub-id-type="doi">10.1093/jxb/ern169</article-id><article-categories><subj-group><subject>Research Papers</subject></subj-group></article-categories><title-group><article-title>GCN2-dependent phosphorylation of eukaryotic translation initiation factor-2α in Arabidopsis</article-title></title-group><contrib-group><contrib contrib-type="author"><name><surname>Zhang</surname><given-names>Yuhua</given-names></name><xref ref-type="aff" rid="aff1">1</xref></contrib><contrib contrib-type="author"><name><surname>Wang</surname><given-names>Yifei</given-names></name><xref ref-type="aff" rid="aff1">1</xref><xref ref-type="fn" rid="fn1">*</xref></contrib><contrib contrib-type="author"><name><surname>Kanyuka</surname><given-names>Kostya</given-names></name><xref ref-type="aff" rid="aff2">2</xref></contrib><contrib contrib-type="author"><name><surname>Parry</surname><given-names>Martin A. J.</given-names></name><xref ref-type="aff" rid="aff1">1</xref></contrib><contrib contrib-type="author"><name><surname>Powers</surname><given-names>Stephen J.</given-names></name><xref ref-type="aff" rid="aff3">3</xref></contrib><contrib contrib-type="author" corresp="yes"><name><surname>Halford</surname><given-names>Nigel G.</given-names></name><xref ref-type="aff" rid="aff1">1</xref><xref ref-type="corresp" rid="cor1">†</xref></contrib></contrib-group><aff id="aff1"><label>1</label>Centre for Crop Genetic Improvement, Plant Sciences Department, Rothamsted Research, Harpenden, Hertfordshire AL5 2JQ, UK</aff><aff id="aff2"><label>2</label>Centre for Sustainable Pest and Disease Management, Plant Pathology and Microbiology Department, Rothamsted Research, Harpenden, Hertfordshire AL5 2JQ, UK</aff><aff id="aff3"><label>3</label>Centre for Mathematical and Computational Biology, Biomathematics and Bioinformatics Department, Rothamsted Research, Harpenden, Hertfordshire AL5 2JQ, UK</aff><author-notes><corresp id="cor1"><label>†</label>To whom correspondence should be addressed. E-mail: <email>nigel.halford@bbsrc.ac.uk</email></corresp><fn id="fn1"><label>*</label><p>Present address: Biotechnology Research Institute, Shanghai Academy of Agricultural Sciences, 2901 Bei Di Road, Shanghai 201106, Peoples Republic of China.</p></fn></author-notes><pub-date pub-type="epub-ppub"><month>8</month><year>2008</year></pub-date><pub-date pub-type="epub"><day>4</day><month>7</month><year>2008</year></pub-date><volume>59</volume><issue>11</issue><fpage>3131</fpage><lpage>3141</lpage><history><date date-type="received"><day>17</day><month>4</month><year>2008</year></date><date date-type="rev-recd"><day>20</day><month>5</month><year>2008</year></date><date date-type="accepted"><day>20</day><month>5</month><year>2008</year></date></history><permissions><copyright-statement>© 2008 The Author(s).</copyright-statement><copyright-year>2008</copyright-year><license license-type="open-access"><p>This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/2.0/uk/) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.</p><p>This paper is available online free of all access charges (see <ext-link ext-link-type="uri" xlink:href="http://jxb.oxfordjournals.org/open_access.html">http://jxb.oxfordjournals.org/open_access.html</ext-link> for further details)</p></license></permissions><abstract><p>The yeast regulatory protein kinase, general control non-derepressible-2 (GCN2) plays a key role in general amino acid control. GCN2 phosphorylates the α subunit of the trimeric eukaryotic translation initiation factor-2 (eIF2), bringing about a decrease in the general rate of protein synthesis but an increase in the synthesis of GCN4, a transcription factor that promotes the expression of genes encoding enzymes for amino acid biosynthesis. The present study concerned the phosphorylation of Arabidopsis eIF2α (AteIF2α) by the Arabidopsis homologue of GCN2, AtGCN2, and the role of AtGCN2 in regulating genes encoding enzymes of amino acid biosynthesis and responding to virus infection. A null mutant for AtGCN2 called GT8359 was obtained and western analysis confirmed that it lacked AtGCN2 protein. GT8359 was more sensitive than wild-type Arabidopsis to herbicides that affect amino acid biosynthesis. Phosphorylation of AteIF2α occurred in response to herbicide treatment but only in wild-type Arabidopsis, not GT8359, showing it to be AtGCN2-dependent. Expression analysis of genes encoding key enzymes for amino acid biosynthesis and nitrate assimilation revealed little effect of loss of AtGCN2 function in GT8359 except that expression of a nitrate reductase gene, <italic>NIA1</italic>, was decreased. Analysis of wild-type and GT8359 plants infected with <italic>Turnip yellow mosaic virus</italic> or <italic>Turnip crinkle virus</italic> showed that AteIF2α was not phosphorylated.</p></abstract><kwd-group><kwd>Amino acid signalling</kwd><kwd>general control non-derepressible</kwd><kwd>metabolic regulation</kwd><kwd>phosphorylation</kwd><kwd>protein kinase</kwd><kwd>virus infection</kwd></kwd-group></article-meta></front></article></istex:document></istex:metadataXml><mods version="3.6"><titleInfo><title>GCN2-dependent phosphorylation of eukaryotic translation initiation factor-2 in Arabidopsis</title></titleInfo><titleInfo type="alternative" contentType="CDATA"><title>GCN2-dependent phosphorylation of eukaryotic translation initiation factor-2 in Arabidopsis</title></titleInfo><name type="personal"><namePart type="given">Yuhua</namePart><namePart type="family">Zhang</namePart><affiliation>Centre for Crop Genetic Improvement, Plant Sciences Department, Rothamsted Research, Harpenden, Hertfordshire AL5 2JQ, UK</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">Yifei</namePart><namePart type="family">Wang</namePart><affiliation>Centre for Crop Genetic Improvement, Plant Sciences Department, Rothamsted Research, Harpenden, Hertfordshire AL5 2JQ, UK</affiliation><description>Present address: Biotechnology Research Institute, Shanghai Academy of Agricultural Sciences, 2901 Bei Di Road, Shanghai 201106, Peoples Republic of China.</description><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">Kostya</namePart><namePart type="family">Kanyuka</namePart><affiliation>Centre for Sustainable Pest and Disease Management, Plant Pathology and Microbiology Department, Rothamsted Research, Harpenden, Hertfordshire AL5 2JQ, UK</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">Martin A. J.</namePart><namePart type="family">Parry</namePart><affiliation>Centre for Crop Genetic Improvement, Plant Sciences Department, Rothamsted Research, Harpenden, Hertfordshire AL5 2JQ, UK</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">Stephen J.</namePart><namePart type="family">Powers</namePart><affiliation>Centre for Mathematical and Computational Biology, Biomathematics and Bioinformatics Department, Rothamsted Research, Harpenden, Hertfordshire AL5 2JQ, UK</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal" displayLabel="corresp"><namePart type="given">Nigel G.</namePart><namePart type="family">Halford</namePart><affiliation>Centre for Crop Genetic Improvement, Plant Sciences Department, Rothamsted Research, Harpenden, Hertfordshire AL5 2JQ, UK</affiliation><affiliation>E-mail: nigel.halford@bbsrc.ac.uk</affiliation><role><roleTerm type="text">author</roleTerm></role></name><typeOfResource>text</typeOfResource><genre type="research-article" displayLabel="research-article"></genre><subject><topic>Research Papers</topic></subject><originInfo><publisher>Oxford University Press</publisher><dateIssued encoding="w3cdtf">2008-07-04</dateIssued><dateCreated encoding="w3cdtf">2008-07-04</dateCreated><copyrightDate encoding="w3cdtf">2008</copyrightDate></originInfo><language><languageTerm type="code" authority="iso639-2b">eng</languageTerm><languageTerm type="code" authority="rfc3066">en</languageTerm></language><physicalDescription><internetMediaType>text/html</internetMediaType></physicalDescription><abstract>The yeast regulatory protein kinase, general control non-derepressible-2 (GCN2) plays a key role in general amino acid control. GCN2 phosphorylates the subunit of the trimeric eukaryotic translation initiation factor-2 (eIF2), bringing about a decrease in the general rate of protein synthesis but an increase in the synthesis of GCN4, a transcription factor that promotes the expression of genes encoding enzymes for amino acid biosynthesis. The present study concerned the phosphorylation of Arabidopsis eIF2 (AteIF2) by the Arabidopsis homologue of GCN2, AtGCN2, and the role of AtGCN2 in regulating genes encoding enzymes of amino acid biosynthesis and responding to virus infection. A null mutant for AtGCN2 called GT8359 was obtained and western analysis confirmed that it lacked AtGCN2 protein. GT8359 was more sensitive than wild-type Arabidopsis to herbicides that affect amino acid biosynthesis. Phosphorylation of AteIF2 occurred in response to herbicide treatment but only in wild-type Arabidopsis, not GT8359, showing it to be AtGCN2-dependent. Expression analysis of genes encoding key enzymes for amino acid biosynthesis and nitrate assimilation revealed little effect of loss of AtGCN2 function in GT8359 except that expression of a nitrate reductase gene, NIA1, was decreased. Analysis of wild-type and GT8359 plants infected with Turnip yellow mosaic virus or Turnip crinkle virus showed that AteIF2 was not phosphorylated.</abstract><note type="footnotes">Present address: Biotechnology Research Institute, Shanghai Academy of Agricultural Sciences, 2901 Bei Di Road, Shanghai 201106, Peoples Republic of China.</note><subject><genre>keywords</genre><topic>Amino acid signalling</topic><topic>general control non-derepressible</topic><topic>metabolic regulation</topic><topic>phosphorylation</topic><topic>protein kinase</topic><topic>virus infection</topic></subject><relatedItem type="host"><titleInfo><title>Journal of Experimental Botany</title></titleInfo><genre type="journal">journal</genre><identifier type="ISSN">0022-0957</identifier><identifier type="eISSN">1460-2431</identifier><identifier type="PublisherID">exbotj</identifier><identifier type="PublisherID-hwp">jexbot</identifier><part><date>2008</date><detail type="volume"><caption>vol.</caption><number>59</number></detail><detail type="issue"><caption>no.</caption><number>11</number></detail><extent unit="pages"><start>3131</start><end>3141</end></extent></part></relatedItem><identifier type="istex">0F0F4A2A2EB5C1448AD78F811381212C0FF6D581</identifier><identifier type="DOI">10.1093/jxb/ern169</identifier><accessCondition type="use and reproduction" contentType="open-access">This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/2.0/uk/) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.</accessCondition><recordInfo><recordContentSource>OUP</recordContentSource></recordInfo></mods></metadata><covers><json:item><original>true</original><mimetype>image/tiff</mimetype><extension>tiff</extension><uri>https://api.istex.fr/document/0F0F4A2A2EB5C1448AD78F811381212C0FF6D581/covers/tiff</uri></json:item><json:item><original>true</original><mimetype>text/html</mimetype><extension>html</extension><uri>https://api.istex.fr/document/0F0F4A2A2EB5C1448AD78F811381212C0FF6D581/covers/html</uri></json:item></covers><annexes><json:item><original>true</original><mimetype>image/jpeg</mimetype><extension>jpeg</extension><uri>https://api.istex.fr/document/0F0F4A2A2EB5C1448AD78F811381212C0FF6D581/annexes/jpeg</uri></json:item><json:item><original>true</original><mimetype>image/gif</mimetype><extension>gif</extension><uri>https://api.istex.fr/document/0F0F4A2A2EB5C1448AD78F811381212C0FF6D581/annexes/gif</uri></json:item><json:item><original>true</original><mimetype>application/pdf</mimetype><extension>pdf</extension><uri>https://api.istex.fr/document/0F0F4A2A2EB5C1448AD78F811381212C0FF6D581/annexes/pdf</uri></json:item></annexes><enrichments><istex:catWosTEI uri="https://api.istex.fr/document/0F0F4A2A2EB5C1448AD78F811381212C0FF6D581/enrichments/catWos"><teiHeader><profileDesc><textClass><classCode scheme="WOS">PLANT SCIENCES</classCode></textClass></profileDesc></teiHeader></istex:catWosTEI><json:item><type>refBibs</type><uri>https://api.istex.fr/document/0F0F4A2A2EB5C1448AD78F811381212C0FF6D581/enrichments/refBibs</uri></json:item></enrichments><serie></serie></istex></record>Pour manipuler ce document sous Unix (Dilib)
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