Recovery of heat shock-triggered released apoplastic Ca2 accompanied by pectin methylesterase activity is required for thermotolerance in soybean seedlings
Identifieur interne : 001499 ( Istex/Corpus ); précédent : 001498; suivant : 001500Recovery of heat shock-triggered released apoplastic Ca2 accompanied by pectin methylesterase activity is required for thermotolerance in soybean seedlings
Auteurs : Hui-Chen Wu ; Shih-Feng Hsu ; Dan-Li Luo ; Shiang-Jiuun Chen ; Wen-Dar Huang ; Huu-Sheng Lur ; Tsung-Luo JinnSource :
- Journal of Experimental Botany [ 0022-0957 ] ; 2010-06.
Abstract
Synthesis of heat shock proteins (HSPs) in response to heat shock (HS) is essential for thermotolerance. The effect of a Ca2 chelator, EGTA, was investigated before a lethal HS treatment in soybean (Glycine max) seedlings with acquired thermotolerance induced by preheating. Such seedlings became non-thermotolerant with EGTA treatment. The addition of Ca2, Sr2 or Ba2 to the EGTA-treated samples rescued the seedlings from death by preventing the increased cellular leakage of electrolytes, amino acids, and sugars caused by EGTA. It was confirmed that EGTA did not affect HSP accumulation and physiological functions but interfered with the recovery of HS-released Ca2 concentration which was required for thermotolerance. Pectin methylesterase (PME, EC 3.1.1.11), a cell wall remodelling enzyme, was activated in response to HS, and its elevated activity caused an increased level of demethylesterified pectin which was related to the recovery of the HS-released Ca2 concentration. Thus, the recovery of HS-released Ca2 in Ca2-pectate reconstitution through PME activity is required for cell wall remodelling during HS in soybean which, in turn, retains plasma membrane integrity and co-ordinates with HSPs to confer thermotolerance.
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DOI: 10.1093/jxb/erq121
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Luo</name><affiliation><mods:affiliation>Institute of Plant Biology and Department of Life Science, National Taiwan University, Taipei 10617, Taiwan</mods:affiliation></affiliation></author><author><name sortKey="Chen, Shiang Jiuun" sort="Chen, Shiang Jiuun" uniqKey="Chen S" first="Shiang-Jiuun" last="Chen">Shiang-Jiuun Chen</name><affiliation><mods:affiliation>Institute of Plant Biology and Department of Life Science, National Taiwan University, Taipei 10617, Taiwan</mods:affiliation></affiliation></author><author><name sortKey="Huang, Wen Dar" sort="Huang, Wen Dar" uniqKey="Huang W" first="Wen-Dar" last="Huang">Wen-Dar Huang</name><affiliation><mods:affiliation>Department of Agronomy, National Taiwan University, Taipei 10617, Taiwan</mods:affiliation></affiliation></author><author><name sortKey="Lur, Huu Sheng" sort="Lur, Huu Sheng" uniqKey="Lur H" first="Huu-Sheng" last="Lur">Huu-Sheng Lur</name><affiliation><mods:affiliation>Department of Agronomy, National Taiwan University, Taipei 10617, Taiwan</mods:affiliation></affiliation></author><author><name sortKey="Jinn, Tsung Luo" sort="Jinn, Tsung Luo" uniqKey="Jinn T" first="Tsung-Luo" last="Jinn">Tsung-Luo Jinn</name><affiliation><mods:affiliation>Institute of Plant Biology and Department of Life Science, National Taiwan University, Taipei 10617, Taiwan</mods:affiliation></affiliation><affiliation><mods:affiliation>E-mail: jinnt@ntu.edu.tw</mods:affiliation></affiliation></author></titleStmt><publicationStmt><idno type="wicri:source">ISTEX</idno><idno type="RBID">ISTEX:B803D941E5DBFB3FA8BB3B2360C33FF9AA806EE0</idno><date when="2010" year="2010">2010</date><idno type="doi">10.1093/jxb/erq121</idno><idno type="url">https://api.istex.fr/document/B803D941E5DBFB3FA8BB3B2360C33FF9AA806EE0/fulltext/pdf</idno><idno type="wicri:Area/Istex/Corpus">001499</idno></publicationStmt><sourceDesc><biblStruct><analytic><title level="a">Recovery of heat shock-triggered released apoplastic Ca2 accompanied by pectin methylesterase activity is required for thermotolerance in soybean seedlings</title><author><name sortKey="Wu, Hui Chen" sort="Wu, Hui Chen" uniqKey="Wu H" first="Hui-Chen" last="Wu">Hui-Chen Wu</name><affiliation><mods:affiliation>Institute of Plant Biology and Department of Life Science, National Taiwan University, Taipei 10617, Taiwan</mods:affiliation></affiliation></author><author><name sortKey="Hsu, Shih Feng" sort="Hsu, Shih Feng" uniqKey="Hsu S" first="Shih-Feng" last="Hsu">Shih-Feng Hsu</name><affiliation><mods:affiliation>Institute of Plant Biology and Department of Life Science, National Taiwan University, Taipei 10617, Taiwan</mods:affiliation></affiliation></author><author><name sortKey="Luo, Dan Li" sort="Luo, Dan Li" uniqKey="Luo D" first="Dan-Li" last="Luo">Dan-Li Luo</name><affiliation><mods:affiliation>Institute of Plant Biology and Department of Life Science, National Taiwan University, Taipei 10617, Taiwan</mods:affiliation></affiliation></author><author><name sortKey="Chen, Shiang Jiuun" sort="Chen, Shiang Jiuun" uniqKey="Chen S" first="Shiang-Jiuun" last="Chen">Shiang-Jiuun Chen</name><affiliation><mods:affiliation>Institute of Plant Biology and Department of Life Science, National Taiwan University, Taipei 10617, Taiwan</mods:affiliation></affiliation></author><author><name sortKey="Huang, Wen Dar" sort="Huang, Wen Dar" uniqKey="Huang W" first="Wen-Dar" last="Huang">Wen-Dar Huang</name><affiliation><mods:affiliation>Department of Agronomy, National Taiwan University, Taipei 10617, Taiwan</mods:affiliation></affiliation></author><author><name sortKey="Lur, Huu Sheng" sort="Lur, Huu Sheng" uniqKey="Lur H" first="Huu-Sheng" last="Lur">Huu-Sheng Lur</name><affiliation><mods:affiliation>Department of Agronomy, National Taiwan University, Taipei 10617, Taiwan</mods:affiliation></affiliation></author><author><name sortKey="Jinn, Tsung Luo" sort="Jinn, Tsung Luo" uniqKey="Jinn T" first="Tsung-Luo" last="Jinn">Tsung-Luo 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The effect of a Ca2 chelator, EGTA, was investigated before a lethal HS treatment in soybean (Glycine max) seedlings with acquired thermotolerance induced by preheating. Such seedlings became non-thermotolerant with EGTA treatment. The addition of Ca2, Sr2 or Ba2 to the EGTA-treated samples rescued the seedlings from death by preventing the increased cellular leakage of electrolytes, amino acids, and sugars caused by EGTA. It was confirmed that EGTA did not affect HSP accumulation and physiological functions but interfered with the recovery of HS-released Ca2 concentration which was required for thermotolerance. Pectin methylesterase (PME, EC 3.1.1.11), a cell wall remodelling enzyme, was activated in response to HS, and its elevated activity caused an increased level of demethylesterified pectin which was related to the recovery of the HS-released Ca2 concentration. Thus, the recovery of HS-released Ca2 in Ca2-pectate reconstitution through PME activity is required for cell wall remodelling during HS in soybean which, in turn, retains plasma membrane integrity and co-ordinates with HSPs to confer thermotolerance.</div></front></TEI><istex><corpusName>oup</corpusName><author><json:item><name>Hui-Chen Wu</name><affiliations><json:string>Institute of Plant Biology and Department of Life Science, National Taiwan University, Taipei 10617, Taiwan</json:string></affiliations></json:item><json:item><name>Shih-Feng Hsu</name><affiliations><json:string>Institute of Plant Biology and Department of Life Science, National Taiwan University, Taipei 10617, Taiwan</json:string></affiliations></json:item><json:item><name>Dan-Li Luo</name><affiliations><json:string>Institute of Plant Biology and Department of Life Science, National Taiwan University, Taipei 10617, Taiwan</json:string></affiliations></json:item><json:item><name>Shiang-Jiuun Chen</name><affiliations><json:string>Institute of Plant Biology and Department of Life Science, National Taiwan University, Taipei 10617, Taiwan</json:string></affiliations></json:item><json:item><name>Wen-Dar Huang</name><affiliations><json:string>Department of Agronomy, National Taiwan University, Taipei 10617, Taiwan</json:string></affiliations></json:item><json:item><name>Huu-Sheng Lur</name><affiliations><json:string>Department of Agronomy, National Taiwan University, Taipei 10617, Taiwan</json:string></affiliations></json:item><json:item><name>Tsung-Luo Jinn</name><affiliations><json:string>Institute of Plant Biology and Department of Life Science, National Taiwan University, Taipei 10617, Taiwan</json:string><json:string>E-mail: jinnt@ntu.edu.tw</json:string></affiliations></json:item></author><subject><json:item><lang><json:string>eng</json:string></lang><value>Research Papers</value></json:item><json:item><lang><json:string>eng</json:string></lang><value>Ca2</value></json:item><json:item><lang><json:string>eng</json:string></lang><value>heat shock</value></json:item><json:item><lang><json:string>eng</json:string></lang><value>pectin</value></json:item><json:item><lang><json:string>eng</json:string></lang><value>pectin methylesterase</value></json:item><json:item><lang><json:string>eng</json:string></lang><value>thermotolerance</value></json:item></subject><language><json:string>eng</json:string></language><originalGenre><json:string>research-article</json:string></originalGenre><abstract>Synthesis of heat shock proteins (HSPs) in response to heat shock (HS) is essential for thermotolerance. The effect of a Ca2 chelator, EGTA, was investigated before a lethal HS treatment in soybean (Glycine max) seedlings with acquired thermotolerance induced by preheating. Such seedlings became non-thermotolerant with EGTA treatment. The addition of Ca2, Sr2 or Ba2 to the EGTA-treated samples rescued the seedlings from death by preventing the increased cellular leakage of electrolytes, amino acids, and sugars caused by EGTA. It was confirmed that EGTA did not affect HSP accumulation and physiological functions but interfered with the recovery of HS-released Ca2 concentration which was required for thermotolerance. Pectin methylesterase (PME, EC 3.1.1.11), a cell wall remodelling enzyme, was activated in response to HS, and its elevated activity caused an increased level of demethylesterified pectin which was related to the recovery of the HS-released Ca2 concentration. Thus, the recovery of HS-released Ca2 in Ca2-pectate reconstitution through PME activity is required for cell wall remodelling during HS in soybean which, in turn, retains plasma membrane integrity and co-ordinates with HSPs to confer thermotolerance.</abstract><qualityIndicators><score>7.16</score><pdfVersion>1.3</pdfVersion><pdfPageSize>612 x 791 pts</pdfPageSize><refBibsNative>false</refBibsNative><keywordCount>6</keywordCount><abstractCharCount>1236</abstractCharCount><pdfWordCount>6071</pdfWordCount><pdfCharCount>38314</pdfCharCount><pdfPageCount>10</pdfPageCount><abstractWordCount>180</abstractWordCount></qualityIndicators><title>Recovery of heat shock-triggered released apoplastic Ca2 accompanied by pectin methylesterase activity is required for thermotolerance in soybean 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The effect of a Ca2 chelator, EGTA, was investigated before a lethal HS treatment in soybean (Glycine max) seedlings with acquired thermotolerance induced by preheating. Such seedlings became non-thermotolerant with EGTA treatment. The addition of Ca2, Sr2 or Ba2 to the EGTA-treated samples rescued the seedlings from death by preventing the increased cellular leakage of electrolytes, amino acids, and sugars caused by EGTA. It was confirmed that EGTA did not affect HSP accumulation and physiological functions but interfered with the recovery of HS-released Ca2 concentration which was required for thermotolerance. Pectin methylesterase (PME, EC 3.1.1.11), a cell wall remodelling enzyme, was activated in response to HS, and its elevated activity caused an increased level of demethylesterified pectin which was related to the recovery of the HS-released Ca2 concentration. Thus, the recovery of HS-released Ca2 in Ca2-pectate reconstitution through PME activity is required for cell wall remodelling during HS in soybean which, in turn, retains plasma membrane integrity and co-ordinates with HSPs to confer thermotolerance.</p></abstract><textClass><keywords scheme="keyword"><list><item><term>Research Papers</term></item></list></keywords></textClass><textClass><keywords scheme="keyword"><list><head>keywords</head><item><term>Ca2</term></item><item><term>heat shock</term></item><item><term>pectin</term></item><item><term>pectin methylesterase</term></item><item><term>thermotolerance</term></item></list></keywords></textClass></profileDesc><revisionDesc><change when="2010-05-05">Created</change><change when="2010-06">Published</change><change xml:id="refBibs-istex" who="#ISTEX-API" when="2016-10-14">References added</change></revisionDesc></teiHeader></istex:fulltextTEI><json:item><original>false</original><mimetype>text/plain</mimetype><extension>txt</extension><uri>https://api.istex.fr/document/B803D941E5DBFB3FA8BB3B2360C33FF9AA806EE0/fulltext/txt</uri></json:item></fulltext><metadata><istex:metadataXml wicri:clean="corpus oup" wicri:toSee="no header"><istex:docType PUBLIC="-//NLM//DTD Journal Publishing DTD v2.3 20070202//EN" URI="journalpublishing.dtd" name="istex:docType"></istex:docType><istex:document><article article-type="research-article"><front><journal-meta><journal-id journal-id-type="hwp">jexbot</journal-id><journal-id journal-id-type="publisher-id">exbotj</journal-id><journal-title>Journal of Experimental Botany</journal-title><issn pub-type="epub">1460-2431</issn><issn pub-type="ppub">0022-0957</issn><publisher><publisher-name>Oxford University Press</publisher-name></publisher></journal-meta><article-meta><article-id pub-id-type="doi">10.1093/jxb/erq121</article-id><article-categories><subj-group><subject>Research Papers</subject></subj-group></article-categories><title-group><article-title>Recovery of heat shock-triggered released apoplastic Ca<sup>2+</sup> accompanied by pectin methylesterase activity is required for thermotolerance in soybean seedlings</article-title></title-group><contrib-group><contrib contrib-type="author"><name><surname>Wu</surname><given-names>Hui-Chen</given-names></name><xref ref-type="aff" rid="aff1">1</xref></contrib><contrib contrib-type="author"><name><surname>Hsu</surname><given-names>Shih-Feng</given-names></name><xref ref-type="aff" rid="aff1">1</xref></contrib><contrib contrib-type="author"><name><surname>Luo</surname><given-names>Dan-Li</given-names></name><xref ref-type="aff" rid="aff1">1</xref></contrib><contrib contrib-type="author"><name><surname>Chen</surname><given-names>Shiang-Jiuun</given-names></name><xref ref-type="aff" rid="aff1">1</xref></contrib><contrib contrib-type="author"><name><surname>Huang</surname><given-names>Wen-Dar</given-names></name><xref ref-type="aff" rid="aff2">2</xref></contrib><contrib contrib-type="author"><name><surname>Lur</surname><given-names>Huu-Sheng</given-names></name><xref ref-type="aff" rid="aff2">2</xref></contrib><contrib contrib-type="author" corresp="yes"><name><surname>Jinn</surname><given-names>Tsung-Luo</given-names></name><xref ref-type="aff" rid="aff1">1</xref><xref ref-type="corresp" rid="cor1">*</xref></contrib></contrib-group><aff id="aff1"><label>1</label>Institute of Plant Biology and Department of Life Science, National Taiwan University, Taipei 10617, Taiwan</aff><aff id="aff2"><label>2</label>Department of Agronomy, National Taiwan University, Taipei 10617, Taiwan</aff><author-notes><corresp id="cor1"><label>*</label>To whom correspondence should be addressed: E-mail: <email>jinnt@ntu.edu.tw</email></corresp></author-notes><pub-date pub-type="ppub"><month>06</month><year>2010</year></pub-date><pub-date pub-type="epub"><day>05</day><month>05</month><year>2010</year></pub-date><volume>61</volume><issue>10</issue><fpage>2843</fpage><lpage>2852</lpage><history><date date-type="received"><day>20</day><month>1</month><year>2010</year></date><date date-type="rev-recd"><day>9</day><month>4</month><year>2010</year></date><date date-type="accepted"><day>12</day><month>4</month><year>2010</year></date></history><permissions><copyright-statement>© 2010 The Author(s).</copyright-statement><copyright-year>2010</copyright-year><license license-type="open-access"><p>This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/2.5), which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.</p><p>This paper is available online free of all access charges (see <ext-link ext-link-type="uri" xlink:href="http://jxb.oxfordjournals.org/open_access.html">http://jxb.oxfordjournals.org/open_access.html</ext-link> for further details)</p></license></permissions><abstract><p>Synthesis of heat shock proteins (HSPs) in response to heat shock (HS) is essential for thermotolerance. The effect of a Ca<sup>2+</sup> chelator, EGTA, was investigated before a lethal HS treatment in soybean (<italic>Glycine max</italic>) seedlings with acquired thermotolerance induced by preheating. Such seedlings became non-thermotolerant with EGTA treatment. The addition of Ca<sup>2+</sup>, Sr<sup>2+</sup> or Ba<sup>2+</sup> to the EGTA-treated samples rescued the seedlings from death by preventing the increased cellular leakage of electrolytes, amino acids, and sugars caused by EGTA. It was confirmed that EGTA did not affect HSP accumulation and physiological functions but interfered with the recovery of HS-released Ca<sup>2+</sup> concentration which was required for thermotolerance. Pectin methylesterase (PME, EC 3.1.1.11), a cell wall remodelling enzyme, was activated in response to HS, and its elevated activity caused an increased level of demethylesterified pectin which was related to the recovery of the HS-released Ca<sup>2+</sup> concentration. Thus, the recovery of HS-released Ca<sup>2+</sup> in Ca<sup>2+</sup>-pectate reconstitution through PME activity is required for cell wall remodelling during HS in soybean which, in turn, retains plasma membrane integrity and co-ordinates with HSPs to confer thermotolerance.</p></abstract><kwd-group><kwd>Ca<sup>2+</sup></kwd><kwd>heat shock</kwd><kwd>pectin</kwd><kwd>pectin methylesterase</kwd><kwd>thermotolerance</kwd></kwd-group></article-meta></front></article></istex:document></istex:metadataXml><mods version="3.6"><titleInfo><title>Recovery of heat shock-triggered released apoplastic Ca2 accompanied by pectin methylesterase activity is required for thermotolerance in soybean seedlings</title></titleInfo><titleInfo type="alternative" contentType="CDATA"><title>Recovery of heat shock-triggered released apoplastic Ca2 accompanied by pectin methylesterase activity is required for thermotolerance in soybean seedlings</title></titleInfo><name type="personal"><namePart type="given">Hui-Chen</namePart><namePart type="family">Wu</namePart><affiliation>Institute of Plant Biology and Department of Life Science, National Taiwan University, Taipei 10617, Taiwan</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">Shih-Feng</namePart><namePart type="family">Hsu</namePart><affiliation>Institute of Plant Biology and Department of Life Science, National Taiwan University, Taipei 10617, Taiwan</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">Dan-Li</namePart><namePart type="family">Luo</namePart><affiliation>Institute of Plant Biology and Department of Life Science, National Taiwan University, Taipei 10617, Taiwan</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">Shiang-Jiuun</namePart><namePart type="family">Chen</namePart><affiliation>Institute of Plant Biology and Department of Life Science, National Taiwan University, Taipei 10617, Taiwan</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">Wen-Dar</namePart><namePart type="family">Huang</namePart><affiliation>Department of Agronomy, National Taiwan University, Taipei 10617, Taiwan</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">Huu-Sheng</namePart><namePart type="family">Lur</namePart><affiliation>Department of Agronomy, National Taiwan University, Taipei 10617, Taiwan</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal" displayLabel="corresp"><namePart type="given">Tsung-Luo</namePart><namePart type="family">Jinn</namePart><affiliation>Institute of Plant Biology and Department of Life Science, National Taiwan University, Taipei 10617, Taiwan</affiliation><affiliation>E-mail: jinnt@ntu.edu.tw</affiliation><role><roleTerm type="text">author</roleTerm></role></name><typeOfResource>text</typeOfResource><genre type="research-article" displayLabel="research-article"></genre><subject><topic>Research Papers</topic></subject><originInfo><publisher>Oxford University Press</publisher><dateIssued encoding="w3cdtf">2010-06</dateIssued><dateCreated encoding="w3cdtf">2010-05-05</dateCreated><copyrightDate encoding="w3cdtf">2010</copyrightDate></originInfo><language><languageTerm type="code" authority="iso639-2b">eng</languageTerm><languageTerm type="code" authority="rfc3066">en</languageTerm></language><physicalDescription><internetMediaType>text/html</internetMediaType></physicalDescription><abstract>Synthesis of heat shock proteins (HSPs) in response to heat shock (HS) is essential for thermotolerance. The effect of a Ca2 chelator, EGTA, was investigated before a lethal HS treatment in soybean (Glycine max) seedlings with acquired thermotolerance induced by preheating. Such seedlings became non-thermotolerant with EGTA treatment. The addition of Ca2, Sr2 or Ba2 to the EGTA-treated samples rescued the seedlings from death by preventing the increased cellular leakage of electrolytes, amino acids, and sugars caused by EGTA. It was confirmed that EGTA did not affect HSP accumulation and physiological functions but interfered with the recovery of HS-released Ca2 concentration which was required for thermotolerance. Pectin methylesterase (PME, EC 3.1.1.11), a cell wall remodelling enzyme, was activated in response to HS, and its elevated activity caused an increased level of demethylesterified pectin which was related to the recovery of the HS-released Ca2 concentration. Thus, the recovery of HS-released Ca2 in Ca2-pectate reconstitution through PME activity is required for cell wall remodelling during HS in soybean which, in turn, retains plasma membrane integrity and co-ordinates with HSPs to confer thermotolerance.</abstract><subject><genre>keywords</genre><topic>Ca2</topic><topic>heat shock</topic><topic>pectin</topic><topic>pectin methylesterase</topic><topic>thermotolerance</topic></subject><relatedItem type="host"><titleInfo><title>Journal of Experimental Botany</title></titleInfo><genre type="journal">journal</genre><identifier type="ISSN">0022-0957</identifier><identifier type="eISSN">1460-2431</identifier><identifier type="PublisherID">exbotj</identifier><identifier type="PublisherID-hwp">jexbot</identifier><part><date>2010</date><detail type="volume"><caption>vol.</caption><number>61</number></detail><detail type="issue"><caption>no.</caption><number>10</number></detail><extent unit="pages"><start>2843</start><end>2852</end></extent></part></relatedItem><identifier type="istex">B803D941E5DBFB3FA8BB3B2360C33FF9AA806EE0</identifier><identifier type="DOI">10.1093/jxb/erq121</identifier><accessCondition type="use and reproduction" contentType="open-access">This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/2.5), which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.</accessCondition><recordInfo><recordContentSource>OUP</recordContentSource></recordInfo></mods></metadata><covers><json:item><original>true</original><mimetype>image/tiff</mimetype><extension>tiff</extension><uri>https://api.istex.fr/document/B803D941E5DBFB3FA8BB3B2360C33FF9AA806EE0/covers/tiff</uri></json:item><json:item><original>true</original><mimetype>text/html</mimetype><extension>html</extension><uri>https://api.istex.fr/document/B803D941E5DBFB3FA8BB3B2360C33FF9AA806EE0/covers/html</uri></json:item></covers><annexes><json:item><original>true</original><mimetype>image/jpeg</mimetype><extension>jpeg</extension><uri>https://api.istex.fr/document/B803D941E5DBFB3FA8BB3B2360C33FF9AA806EE0/annexes/jpeg</uri></json:item><json:item><original>true</original><mimetype>image/gif</mimetype><extension>gif</extension><uri>https://api.istex.fr/document/B803D941E5DBFB3FA8BB3B2360C33FF9AA806EE0/annexes/gif</uri></json:item><json:item><original>true</original><mimetype>application/pdf</mimetype><extension>pdf</extension><uri>https://api.istex.fr/document/B803D941E5DBFB3FA8BB3B2360C33FF9AA806EE0/annexes/pdf</uri></json:item></annexes><enrichments><istex:catWosTEI uri="https://api.istex.fr/document/B803D941E5DBFB3FA8BB3B2360C33FF9AA806EE0/enrichments/catWos"><teiHeader><profileDesc><textClass><classCode scheme="WOS">PLANT SCIENCES</classCode></textClass></profileDesc></teiHeader></istex:catWosTEI><json:item><type>refBibs</type><uri>https://api.istex.fr/document/B803D941E5DBFB3FA8BB3B2360C33FF9AA806EE0/enrichments/refBibs</uri></json:item></enrichments><serie></serie></istex></record>Pour manipuler ce document sous Unix (Dilib)
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