The ectopically parting cells 1-2 (epc1-2) mutant exhibits an exaggerated response to abscisic acid
Identifieur interne : 001488 ( Istex/Corpus ); précédent : 001487; suivant : 001489The ectopically parting cells 1-2 (epc1-2) mutant exhibits an exaggerated response to abscisic acid
Auteurs : Lindsay Bown ; Shinnosuke Kusaba ; Florence Goubet ; Lesley Codrai ; Aaron G. Dale ; Zhinong Zhang ; Xiaolan Yu ; Karl Morris ; Tadashi Ishii ; Carol Evered ; Paul Dupree ; Stephen JacksonSource :
- Journal of Experimental Botany [ 0022-0957 ] ; 2007-04-10.
Abstract
The ECTOPICALLY PARTING CELLS 1 (EPC1) gene encodes a putative retaining glycosyltransferase of the GT64 family, and epc1-1 mutant plants have a severely dwarfed phenotype. A new mutant allele of this gene, epc1-2, has been isolated. Reduced cell adhesion that has previously been reported for the epc1-1 mutant was not observed for either the epc1-1 or epc1-2 mutants grown in our conditions, suggesting that EPC1 does not affect cell adhesion but is involved in some other process affecting plant growth and development. It is shown that the epc1-2 mutant exhibits hypersensitivity to the phytohormone abscisic acid in germination and root elongation assays, however it shows an unaltered response to gibberellin, epi-brassinosteroid, auxin, or ethylene. An EPC1:YFP fusion protein is localized to small motile structures within the cytosol that are similar in size and number to the Golgi apparatus. Analysis of cell wall pectins revealed that levels of -(1,4)-galactan in the epc1-2 mutant are reduced by 50%, whilst other pectic polysaccharides (homogalacturonan, arabinan, and rhamnogalacturonan II) are unchanged.
Url:
DOI: 10.1093/jxb/erm040
Links to Exploration step
ISTEX:AAB09354B7951E12A7302D3B2D698657CC1565B1Le document en format XML
<record><TEI wicri:istexFullTextTei="biblStruct"><teiHeader><fileDesc><titleStmt><title>The ectopically parting cells 1-2 (epc1-2) mutant exhibits an exaggerated response to abscisic acid</title><author><name sortKey="Bown, Lindsay" sort="Bown, Lindsay" uniqKey="Bown L" first="Lindsay" last="Bown">Lindsay Bown</name><affiliation><mods:affiliation>Warwick HRI, University of Warwick, Wellesbourne, Warwick CV35 9EF, UK</mods:affiliation></affiliation></author><author><name sortKey="Kusaba, Shinnosuke" sort="Kusaba, Shinnosuke" uniqKey="Kusaba S" first="Shinnosuke" last="Kusaba">Shinnosuke Kusaba</name><affiliation><mods:affiliation>Warwick HRI, University of Warwick, Wellesbourne, Warwick CV35 9EF, UK</mods:affiliation></affiliation><affiliation><mods:affiliation>E-mail: stephen.jackson@warwick.ac.uk</mods:affiliation></affiliation></author><author><name sortKey="Goubet, Florence" sort="Goubet, Florence" uniqKey="Goubet F" first="Florence" last="Goubet">Florence Goubet</name><affiliation><mods:affiliation>Department of Biochemistry, Downing site, Cambridge CB2 1QW, UK</mods:affiliation></affiliation></author><author><name sortKey="Codrai, Lesley" sort="Codrai, Lesley" uniqKey="Codrai L" first="Lesley" last="Codrai">Lesley Codrai</name><affiliation><mods:affiliation>Warwick HRI, University of Warwick, Wellesbourne, Warwick CV35 9EF, UK</mods:affiliation></affiliation></author><author><name sortKey="Dale, Aaron G" sort="Dale, Aaron G" uniqKey="Dale A" first="Aaron G." last="Dale">Aaron G. Dale</name><affiliation><mods:affiliation>Department of Biochemistry, Downing site, Cambridge CB2 1QW, UK</mods:affiliation></affiliation></author><author><name sortKey="Zhang, Zhinong" sort="Zhang, Zhinong" uniqKey="Zhang Z" first="Zhinong" last="Zhang">Zhinong Zhang</name><affiliation><mods:affiliation>Department of Biochemistry, Downing site, Cambridge CB2 1QW, UK</mods:affiliation></affiliation></author><author><name sortKey="Yu, Xiaolan" sort="Yu, Xiaolan" uniqKey="Yu X" first="Xiaolan" last="Yu">Xiaolan Yu</name><affiliation><mods:affiliation>Department of Biochemistry, Downing site, Cambridge CB2 1QW, UK</mods:affiliation></affiliation></author><author><name sortKey="Morris, Karl" sort="Morris, Karl" uniqKey="Morris K" first="Karl" last="Morris">Karl Morris</name><affiliation><mods:affiliation>Warwick HRI, University of Warwick, Wellesbourne, Warwick CV35 9EF, UK</mods:affiliation></affiliation></author><author><name sortKey="Ishii, Tadashi" sort="Ishii, Tadashi" uniqKey="Ishii T" first="Tadashi" last="Ishii">Tadashi Ishii</name><affiliation><mods:affiliation>Forestry and Forest Products Research Institute, 1 Matunosato, Tsukuba, Ibaraki, 305-8687, Japan</mods:affiliation></affiliation></author><author><name sortKey="Evered, Carol" sort="Evered, Carol" uniqKey="Evered C" first="Carol" last="Evered">Carol Evered</name><affiliation><mods:affiliation>Warwick HRI, University of Warwick, Wellesbourne, Warwick CV35 9EF, UK</mods:affiliation></affiliation></author><author><name sortKey="Dupree, Paul" sort="Dupree, Paul" uniqKey="Dupree P" first="Paul" last="Dupree">Paul Dupree</name><affiliation><mods:affiliation>Department of Biochemistry, Downing site, Cambridge CB2 1QW, UK</mods:affiliation></affiliation></author><author><name sortKey="Jackson, Stephen" sort="Jackson, Stephen" uniqKey="Jackson S" first="Stephen" last="Jackson">Stephen Jackson</name><affiliation><mods:affiliation>Warwick HRI, University of Warwick, Wellesbourne, Warwick CV35 9EF, UK</mods:affiliation></affiliation><affiliation><mods:affiliation>E-mail: stephen.jackson@warwick.ac.uk</mods:affiliation></affiliation></author></titleStmt><publicationStmt><idno type="wicri:source">ISTEX</idno><idno type="RBID">ISTEX:AAB09354B7951E12A7302D3B2D698657CC1565B1</idno><date when="2007" year="2007">2007</date><idno type="doi">10.1093/jxb/erm040</idno><idno type="url">https://api.istex.fr/document/AAB09354B7951E12A7302D3B2D698657CC1565B1/fulltext/pdf</idno><idno type="wicri:Area/Istex/Corpus">001488</idno></publicationStmt><sourceDesc><biblStruct><analytic><title level="a">The ectopically parting cells 1-2 (epc1-2) mutant exhibits an exaggerated response to abscisic acid</title><author><name sortKey="Bown, Lindsay" sort="Bown, Lindsay" uniqKey="Bown L" first="Lindsay" last="Bown">Lindsay Bown</name><affiliation><mods:affiliation>Warwick HRI, University of Warwick, Wellesbourne, Warwick CV35 9EF, UK</mods:affiliation></affiliation></author><author><name sortKey="Kusaba, Shinnosuke" sort="Kusaba, Shinnosuke" uniqKey="Kusaba S" first="Shinnosuke" last="Kusaba">Shinnosuke Kusaba</name><affiliation><mods:affiliation>Warwick HRI, University of Warwick, Wellesbourne, Warwick CV35 9EF, UK</mods:affiliation></affiliation><affiliation><mods:affiliation>E-mail: stephen.jackson@warwick.ac.uk</mods:affiliation></affiliation></author><author><name sortKey="Goubet, Florence" sort="Goubet, Florence" uniqKey="Goubet F" first="Florence" last="Goubet">Florence Goubet</name><affiliation><mods:affiliation>Department of Biochemistry, Downing site, Cambridge CB2 1QW, UK</mods:affiliation></affiliation></author><author><name sortKey="Codrai, Lesley" sort="Codrai, Lesley" uniqKey="Codrai L" first="Lesley" last="Codrai">Lesley Codrai</name><affiliation><mods:affiliation>Warwick HRI, University of Warwick, Wellesbourne, Warwick CV35 9EF, UK</mods:affiliation></affiliation></author><author><name sortKey="Dale, Aaron G" sort="Dale, Aaron G" uniqKey="Dale A" first="Aaron G." last="Dale">Aaron G. Dale</name><affiliation><mods:affiliation>Department of Biochemistry, Downing site, Cambridge CB2 1QW, UK</mods:affiliation></affiliation></author><author><name sortKey="Zhang, Zhinong" sort="Zhang, Zhinong" uniqKey="Zhang Z" first="Zhinong" last="Zhang">Zhinong Zhang</name><affiliation><mods:affiliation>Department of Biochemistry, Downing site, Cambridge CB2 1QW, UK</mods:affiliation></affiliation></author><author><name sortKey="Yu, Xiaolan" sort="Yu, Xiaolan" uniqKey="Yu X" first="Xiaolan" last="Yu">Xiaolan Yu</name><affiliation><mods:affiliation>Department of Biochemistry, Downing site, Cambridge CB2 1QW, UK</mods:affiliation></affiliation></author><author><name sortKey="Morris, Karl" sort="Morris, Karl" uniqKey="Morris K" first="Karl" last="Morris">Karl Morris</name><affiliation><mods:affiliation>Warwick HRI, University of Warwick, Wellesbourne, Warwick CV35 9EF, UK</mods:affiliation></affiliation></author><author><name sortKey="Ishii, Tadashi" sort="Ishii, Tadashi" uniqKey="Ishii T" first="Tadashi" last="Ishii">Tadashi Ishii</name><affiliation><mods:affiliation>Forestry and Forest Products Research Institute, 1 Matunosato, Tsukuba, Ibaraki, 305-8687, Japan</mods:affiliation></affiliation></author><author><name sortKey="Evered, Carol" sort="Evered, Carol" uniqKey="Evered C" first="Carol" last="Evered">Carol Evered</name><affiliation><mods:affiliation>Warwick HRI, University of Warwick, Wellesbourne, Warwick CV35 9EF, UK</mods:affiliation></affiliation></author><author><name sortKey="Dupree, Paul" sort="Dupree, Paul" uniqKey="Dupree P" first="Paul" last="Dupree">Paul Dupree</name><affiliation><mods:affiliation>Department of Biochemistry, Downing site, Cambridge CB2 1QW, UK</mods:affiliation></affiliation></author><author><name sortKey="Jackson, Stephen" sort="Jackson, Stephen" uniqKey="Jackson S" first="Stephen" last="Jackson">Stephen Jackson</name><affiliation><mods:affiliation>Warwick HRI, University of Warwick, Wellesbourne, Warwick CV35 9EF, UK</mods:affiliation></affiliation><affiliation><mods:affiliation>E-mail: stephen.jackson@warwick.ac.uk</mods:affiliation></affiliation></author></analytic><monogr></monogr><series><title level="j">Journal of Experimental Botany</title><idno type="ISSN">0022-0957</idno><idno type="eISSN">1460-2431</idno><imprint><publisher>Oxford University Press</publisher><date type="published" when="2007-04-10">2007-04-10</date><biblScope unit="volume">58</biblScope><biblScope unit="issue">7</biblScope><biblScope unit="page" from="1813">1813</biblScope><biblScope unit="page" to="1823">1823</biblScope></imprint><idno type="ISSN">0022-0957</idno></series><idno type="istex">AAB09354B7951E12A7302D3B2D698657CC1565B1</idno><idno type="DOI">10.1093/jxb/erm040</idno></biblStruct></sourceDesc><seriesStmt><idno type="ISSN">0022-0957</idno></seriesStmt></fileDesc><profileDesc><textClass></textClass><langUsage><language ident="en">en</language></langUsage></profileDesc></teiHeader><front><div type="abstract">The ECTOPICALLY PARTING CELLS 1 (EPC1) gene encodes a putative retaining glycosyltransferase of the GT64 family, and epc1-1 mutant plants have a severely dwarfed phenotype. A new mutant allele of this gene, epc1-2, has been isolated. Reduced cell adhesion that has previously been reported for the epc1-1 mutant was not observed for either the epc1-1 or epc1-2 mutants grown in our conditions, suggesting that EPC1 does not affect cell adhesion but is involved in some other process affecting plant growth and development. It is shown that the epc1-2 mutant exhibits hypersensitivity to the phytohormone abscisic acid in germination and root elongation assays, however it shows an unaltered response to gibberellin, epi-brassinosteroid, auxin, or ethylene. An EPC1:YFP fusion protein is localized to small motile structures within the cytosol that are similar in size and number to the Golgi apparatus. Analysis of cell wall pectins revealed that levels of -(1,4)-galactan in the epc1-2 mutant are reduced by 50%, whilst other pectic polysaccharides (homogalacturonan, arabinan, and rhamnogalacturonan II) are unchanged.</div></front></TEI><istex><corpusName>oup</corpusName><author><json:item><name>Lindsay Bown</name><affiliations><json:string>Warwick HRI, University of Warwick, Wellesbourne, Warwick CV35 9EF, UK</json:string></affiliations></json:item><json:item><name>Shinnosuke Kusaba</name><affiliations><json:string>Warwick HRI, University of Warwick, Wellesbourne, Warwick CV35 9EF, UK</json:string><json:string>E-mail: stephen.jackson@warwick.ac.uk</json:string></affiliations></json:item><json:item><name>Florence Goubet</name><affiliations><json:string>Department of Biochemistry, Downing site, Cambridge CB2 1QW, UK</json:string></affiliations></json:item><json:item><name>Lesley Codrai</name><affiliations><json:string>Warwick HRI, University of Warwick, Wellesbourne, Warwick CV35 9EF, UK</json:string></affiliations></json:item><json:item><name>Aaron G. Dale</name><affiliations><json:string>Department of Biochemistry, Downing site, Cambridge CB2 1QW, UK</json:string></affiliations></json:item><json:item><name>Zhinong Zhang</name><affiliations><json:string>Department of Biochemistry, Downing site, Cambridge CB2 1QW, UK</json:string></affiliations></json:item><json:item><name>Xiaolan Yu</name><affiliations><json:string>Department of Biochemistry, Downing site, Cambridge CB2 1QW, UK</json:string></affiliations></json:item><json:item><name>Karl Morris</name><affiliations><json:string>Warwick HRI, University of Warwick, Wellesbourne, Warwick CV35 9EF, UK</json:string></affiliations></json:item><json:item><name>Tadashi Ishii</name><affiliations><json:string>Forestry and Forest Products Research Institute, 1 Matunosato, Tsukuba, Ibaraki, 305-8687, Japan</json:string></affiliations></json:item><json:item><name>Carol Evered</name><affiliations><json:string>Warwick HRI, University of Warwick, Wellesbourne, Warwick CV35 9EF, UK</json:string></affiliations></json:item><json:item><name>Paul Dupree</name><affiliations><json:string>Department of Biochemistry, Downing site, Cambridge CB2 1QW, UK</json:string></affiliations></json:item><json:item><name>Stephen Jackson</name><affiliations><json:string>Warwick HRI, University of Warwick, Wellesbourne, Warwick CV35 9EF, UK</json:string><json:string>E-mail: stephen.jackson@warwick.ac.uk</json:string></affiliations></json:item></author><subject><json:item><lang><json:string>eng</json:string></lang><value>Research Papers</value></json:item><json:item><lang><json:string>eng</json:string></lang><value>ABA</value></json:item><json:item><lang><json:string>eng</json:string></lang><value>cell wall</value></json:item><json:item><lang><json:string>eng</json:string></lang><value>dwarf</value></json:item><json:item><lang><json:string>eng</json:string></lang><value>galactan</value></json:item><json:item><lang><json:string>eng</json:string></lang><value>pectin</value></json:item><json:item><lang><json:string>eng</json:string></lang><value>root hair</value></json:item></subject><language><json:string>eng</json:string></language><originalGenre><json:string>research-article</json:string></originalGenre><abstract>The ECTOPICALLY PARTING CELLS 1 (EPC1) gene encodes a putative retaining glycosyltransferase of the GT64 family, and epc1-1 mutant plants have a severely dwarfed phenotype. A new mutant allele of this gene, epc1-2, has been isolated. Reduced cell adhesion that has previously been reported for the epc1-1 mutant was not observed for either the epc1-1 or epc1-2 mutants grown in our conditions, suggesting that EPC1 does not affect cell adhesion but is involved in some other process affecting plant growth and development. It is shown that the epc1-2 mutant exhibits hypersensitivity to the phytohormone abscisic acid in germination and root elongation assays, however it shows an unaltered response to gibberellin, epi-brassinosteroid, auxin, or ethylene. An EPC1:YFP fusion protein is localized to small motile structures within the cytosol that are similar in size and number to the Golgi apparatus. Analysis of cell wall pectins revealed that levels of -(1,4)-galactan in the epc1-2 mutant are reduced by 50%, whilst other pectic polysaccharides (homogalacturonan, arabinan, and rhamnogalacturonan II) are unchanged.</abstract><qualityIndicators><score>7.004</score><pdfVersion>1.3</pdfVersion><pdfPageSize>612 x 791 pts</pdfPageSize><refBibsNative>false</refBibsNative><keywordCount>7</keywordCount><abstractCharCount>1120</abstractCharCount><pdfWordCount>7057</pdfWordCount><pdfCharCount>43345</pdfCharCount><pdfPageCount>11</pdfPageCount><abstractWordCount>167</abstractWordCount></qualityIndicators><title>The ectopically parting cells 1-2 (epc1-2) mutant exhibits an exaggerated response to abscisic acid</title><genre><json:string>research-article</json:string></genre><host><volume>58</volume><publisherId><json:string>exbotj</json:string></publisherId><pages><last>1823</last><first>1813</first></pages><issn><json:string>0022-0957</json:string></issn><issue>7</issue><genre><json:string>journal</json:string></genre><language><json:string>unknown</json:string></language><eissn><json:string>1460-2431</json:string></eissn><title>Journal of Experimental Botany</title></host><categories><wos><json:string>PLANT SCIENCES</json:string></wos></categories><publicationDate>2007</publicationDate><copyrightDate>2007</copyrightDate><doi><json:string>10.1093/jxb/erm040</json:string></doi><id>AAB09354B7951E12A7302D3B2D698657CC1565B1</id><score>0.2264078</score><fulltext><json:item><original>true</original><mimetype>application/pdf</mimetype><extension>pdf</extension><uri>https://api.istex.fr/document/AAB09354B7951E12A7302D3B2D698657CC1565B1/fulltext/pdf</uri></json:item><json:item><original>false</original><mimetype>application/zip</mimetype><extension>zip</extension><uri>https://api.istex.fr/document/AAB09354B7951E12A7302D3B2D698657CC1565B1/fulltext/zip</uri></json:item><istex:fulltextTEI uri="https://api.istex.fr/document/AAB09354B7951E12A7302D3B2D698657CC1565B1/fulltext/tei"><teiHeader><fileDesc><titleStmt><title level="a">The ectopically parting cells 1-2 (epc1-2) mutant exhibits an exaggerated response to abscisic acid</title><respStmt><resp>Références bibliographiques récupérées via GROBID</resp><name resp="ISTEX-API">ISTEX-API (INIST-CNRS)</name></respStmt></titleStmt><publicationStmt><authority>ISTEX</authority><publisher>Oxford University Press</publisher><availability status="free"><p>Open Access</p></availability><date>2007-04-10</date></publicationStmt><notesStmt><note>Present address: National Agricultural Research Center for Western Region 2575 Ikano, Zentsuji, Kagawa 765-0053, Japan.</note></notesStmt><sourceDesc><biblStruct type="inbook"><analytic><title level="a">The ectopically parting cells 1-2 (epc1-2) mutant exhibits an exaggerated response to abscisic acid</title><author xml:id="author-1"><persName><forename type="first">Lindsay</forename><surname>Bown</surname></persName><affiliation>Warwick HRI, University of Warwick, Wellesbourne, Warwick CV35 9EF, UK</affiliation></author><author xml:id="author-2" corresp="yes"><persName><forename type="first">Shinnosuke</forename><surname>Kusaba</surname></persName><email>stephen.jackson@warwick.ac.uk</email><note type="biography">Present address: National Agricultural Research Center for Western Region 2575 Ikano, Zentsuji, Kagawa 765-0053, Japan.</note><affiliation>Present address: National Agricultural Research Center for Western Region 2575 Ikano, Zentsuji, Kagawa 765-0053, Japan.</affiliation><affiliation>Warwick HRI, University of Warwick, Wellesbourne, Warwick CV35 9EF, UK</affiliation></author><author xml:id="author-3"><persName><forename type="first">Florence</forename><surname>Goubet</surname></persName><note type="biography">Present address: Bayer BioScience NV, Technologiepark 38, B-9052 Gent, Belgium.</note><affiliation>Present address: Bayer BioScience NV, Technologiepark 38, B-9052 Gent, Belgium.</affiliation><affiliation>Department of Biochemistry, Downing site, Cambridge CB2 1QW, UK</affiliation></author><author xml:id="author-4"><persName><forename type="first">Lesley</forename><surname>Codrai</surname></persName><affiliation>Warwick HRI, University of Warwick, Wellesbourne, Warwick CV35 9EF, UK</affiliation></author><author xml:id="author-5"><persName><forename type="first">Aaron G.</forename><surname>Dale</surname></persName><affiliation>Department of Biochemistry, Downing site, Cambridge CB2 1QW, UK</affiliation></author><author xml:id="author-6"><persName><forename type="first">Zhinong</forename><surname>Zhang</surname></persName><affiliation>Department of Biochemistry, Downing site, Cambridge CB2 1QW, UK</affiliation></author><author xml:id="author-7"><persName><forename type="first">Xiaolan</forename><surname>Yu</surname></persName><affiliation>Department of Biochemistry, Downing site, Cambridge CB2 1QW, UK</affiliation></author><author xml:id="author-8"><persName><forename type="first">Karl</forename><surname>Morris</surname></persName><affiliation>Warwick HRI, University of Warwick, Wellesbourne, Warwick CV35 9EF, UK</affiliation></author><author xml:id="author-9"><persName><forename type="first">Tadashi</forename><surname>Ishii</surname></persName><affiliation>Forestry and Forest Products Research Institute, 1 Matunosato, Tsukuba, Ibaraki, 305-8687, Japan</affiliation></author><author xml:id="author-10"><persName><forename type="first">Carol</forename><surname>Evered</surname></persName><affiliation>Warwick HRI, University of Warwick, Wellesbourne, Warwick CV35 9EF, UK</affiliation></author><author xml:id="author-11"><persName><forename type="first">Paul</forename><surname>Dupree</surname></persName><affiliation>Department of Biochemistry, Downing site, Cambridge CB2 1QW, UK</affiliation></author><author xml:id="author-12" corresp="yes"><persName><forename type="first">Stephen</forename><surname>Jackson</surname></persName><email>stephen.jackson@warwick.ac.uk</email><affiliation>Warwick HRI, University of Warwick, Wellesbourne, Warwick CV35 9EF, UK</affiliation></author></analytic><monogr><title level="j">Journal of Experimental Botany</title><idno type="pISSN">0022-0957</idno><idno type="eISSN">1460-2431</idno><imprint><publisher>Oxford University Press</publisher><date type="published" when="2007-04-10"></date><biblScope unit="volume">58</biblScope><biblScope unit="issue">7</biblScope><biblScope unit="page" from="1813">1813</biblScope><biblScope unit="page" to="1823">1823</biblScope></imprint></monogr><idno type="istex">AAB09354B7951E12A7302D3B2D698657CC1565B1</idno><idno type="DOI">10.1093/jxb/erm040</idno></biblStruct></sourceDesc></fileDesc><profileDesc><creation><date>2007-04-10</date></creation><langUsage><language ident="en">en</language></langUsage><abstract><p>The ECTOPICALLY PARTING CELLS 1 (EPC1) gene encodes a putative retaining glycosyltransferase of the GT64 family, and epc1-1 mutant plants have a severely dwarfed phenotype. A new mutant allele of this gene, epc1-2, has been isolated. Reduced cell adhesion that has previously been reported for the epc1-1 mutant was not observed for either the epc1-1 or epc1-2 mutants grown in our conditions, suggesting that EPC1 does not affect cell adhesion but is involved in some other process affecting plant growth and development. It is shown that the epc1-2 mutant exhibits hypersensitivity to the phytohormone abscisic acid in germination and root elongation assays, however it shows an unaltered response to gibberellin, epi-brassinosteroid, auxin, or ethylene. An EPC1:YFP fusion protein is localized to small motile structures within the cytosol that are similar in size and number to the Golgi apparatus. Analysis of cell wall pectins revealed that levels of -(1,4)-galactan in the epc1-2 mutant are reduced by 50%, whilst other pectic polysaccharides (homogalacturonan, arabinan, and rhamnogalacturonan II) are unchanged.</p></abstract><textClass><keywords scheme="keyword"><list><item><term>Research Papers</term></item></list></keywords></textClass><textClass><keywords scheme="keyword"><list><head>keywords</head><item><term>ABA</term></item><item><term>cell wall</term></item><item><term>dwarf</term></item><item><term>galactan</term></item><item><term>pectin</term></item><item><term>root hair</term></item></list></keywords></textClass></profileDesc><revisionDesc><change when="2007-04-10">Created</change><change when="2007-04-10">Published</change><change xml:id="refBibs-istex" who="#ISTEX-API" when="2016-10-14">References added</change></revisionDesc></teiHeader></istex:fulltextTEI><json:item><original>false</original><mimetype>text/plain</mimetype><extension>txt</extension><uri>https://api.istex.fr/document/AAB09354B7951E12A7302D3B2D698657CC1565B1/fulltext/txt</uri></json:item></fulltext><metadata><istex:metadataXml wicri:clean="corpus oup" wicri:toSee="no header"><istex:docType PUBLIC="-//NLM//DTD Journal Publishing DTD v2.3 20070202//EN" URI="journalpublishing.dtd" name="istex:docType"></istex:docType><istex:document><article article-type="research-article"><front><journal-meta><journal-id journal-id-type="hwp">jexbot</journal-id><journal-id journal-id-type="publisher-id">exbotj</journal-id><journal-title>Journal of Experimental Botany</journal-title><issn pub-type="epub">1460-2431</issn><issn pub-type="ppub">0022-0957</issn><publisher><publisher-name>Oxford University Press</publisher-name></publisher></journal-meta><article-meta><article-id pub-id-type="doi">10.1093/jxb/erm040</article-id><article-categories><subj-group><subject>Research Papers</subject></subj-group></article-categories><title-group><article-title>The <italic>ectopically parting cells 1-2</italic> (<italic>epc1-2</italic>) mutant exhibits an exaggerated response to abscisic acid</article-title></title-group><contrib-group><contrib contrib-type="author"><name><surname>Bown</surname><given-names>Lindsay</given-names></name><xref ref-type="aff" rid="aff1">1</xref></contrib><contrib contrib-type="author" corresp="yes"><name><surname>Kusaba</surname><given-names>Shinnosuke</given-names></name><xref ref-type="aff" rid="aff1">1</xref><xref ref-type="fn" rid="fn1">*</xref></contrib><contrib contrib-type="author"><name><surname>Goubet</surname><given-names>Florence</given-names></name><xref ref-type="aff" rid="aff2">2</xref><xref ref-type="fn" rid="fn2">†</xref></contrib><contrib contrib-type="author"><name><surname>Codrai</surname><given-names>Lesley</given-names></name><xref ref-type="aff" rid="aff1">1</xref></contrib><contrib contrib-type="author"><name><surname>Dale</surname><given-names>Aaron G.</given-names></name><xref ref-type="aff" rid="aff2">2</xref></contrib><contrib contrib-type="author"><name><surname>Zhang</surname><given-names>Zhinong</given-names></name><xref ref-type="aff" rid="aff2">2</xref></contrib><contrib contrib-type="author"><name><surname>Yu</surname><given-names>Xiaolan</given-names></name><xref ref-type="aff" rid="aff2">2</xref></contrib><contrib contrib-type="author"><name><surname>Morris</surname><given-names>Karl</given-names></name><xref ref-type="aff" rid="aff1">1</xref></contrib><contrib contrib-type="author"><name><surname>Ishii</surname><given-names>Tadashi</given-names></name><xref ref-type="aff" rid="aff3">3</xref></contrib><contrib contrib-type="author"><name><surname>Evered</surname><given-names>Carol</given-names></name><xref ref-type="aff" rid="aff1">1</xref></contrib><contrib contrib-type="author"><name><surname>Dupree</surname><given-names>Paul</given-names></name><xref ref-type="aff" rid="aff2">2</xref></contrib><contrib contrib-type="author" corresp="yes"><name><surname>Jackson</surname><given-names>Stephen</given-names></name><xref ref-type="aff" rid="aff1">1</xref><xref ref-type="corresp" rid="cor1">‡</xref></contrib></contrib-group><aff id="aff1"><label>1</label>Warwick HRI, University of Warwick, Wellesbourne, Warwick CV35 9EF, UK</aff><aff id="aff2"><label>2</label>Department of Biochemistry, Downing site, Cambridge CB2 1QW, UK</aff><aff id="aff3"><label>3</label>Forestry and Forest Products Research Institute, 1 Matunosato, Tsukuba, Ibaraki, 305-8687, Japan</aff><author-notes><corresp id="cor1"><label>‡</label>To whom correspondence should be addressed. E-mail: <email>stephen.jackson@warwick.ac.uk</email></corresp><fn id="fn1"><label>*</label><p>Present address: National Agricultural Research Center for Western Region 2575 Ikano, Zentsuji, Kagawa 765-0053, Japan.</p></fn><fn id="fn2"><label>†</label><p>Present address: Bayer BioScience NV, Technologiepark 38, B-9052 Gent, Belgium.</p></fn></author-notes><pub-date pub-type="epub-ppub"><month>5</month><year>2007</year></pub-date><pub-date pub-type="epub"><day>10</day><month>4</month><year>2007</year></pub-date><volume>58</volume><issue>7</issue><fpage>1813</fpage><lpage>1823</lpage><history><date date-type="received"><day>6</day><month>12</month><year>2006</year></date><date date-type="rev-recd"><day>14</day><month>2</month><year>2007</year></date><date date-type="accepted"><day>14</day><month>2</month><year>2007</year></date></history><permissions><copyright-statement>© 2007 The Author(s).</copyright-statement><copyright-year>2007</copyright-year><license license-type="open-access"><p>This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/2.0/uk/) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.</p><p>This paper is available online free of all access charges (see <ext-link ext-link-type="uri" xlink:href="http://jxb.oxfordjournals.org/open_access.html">http://jxb.oxfordjournals.org/open_access.html</ext-link> for further details)</p></license></permissions><abstract><p>The <italic>ECTOPICALLY PARTING CELLS 1 (EPC1</italic>) gene encodes a putative retaining glycosyltransferase of the GT64 family, and <italic>epc1-1</italic> mutant plants have a severely dwarfed phenotype. A new mutant allele of this gene, <italic>epc1-2</italic>, has been isolated. Reduced cell adhesion that has previously been reported for the <italic>epc1-1</italic> mutant was not observed for either the <italic>epc1-1</italic> or <italic>epc1-2</italic> mutants grown in our conditions, suggesting that <italic>EPC1</italic> does not affect cell adhesion but is involved in some other process affecting plant growth and development. It is shown that the <italic>epc1-2</italic> mutant exhibits hypersensitivity to the phytohormone abscisic acid in germination and root elongation assays, however it shows an unaltered response to gibberellin, epi-brassinosteroid, auxin, or ethylene. An EPC1:YFP fusion protein is localized to small motile structures within the cytosol that are similar in size and number to the Golgi apparatus. Analysis of cell wall pectins revealed that levels of β-(1,4)-galactan in the <italic>epc1-2</italic> mutant are reduced by 50%, whilst other pectic polysaccharides (homogalacturonan, arabinan, and rhamnogalacturonan II) are unchanged.</p></abstract><kwd-group><kwd>ABA</kwd><kwd>cell wall</kwd><kwd>dwarf</kwd><kwd>galactan</kwd><kwd>pectin</kwd><kwd>root hair</kwd></kwd-group></article-meta></front></article></istex:document></istex:metadataXml><mods version="3.6"><titleInfo><title>The ectopically parting cells 1-2 (epc1-2) mutant exhibits an exaggerated response to abscisic acid</title></titleInfo><titleInfo type="alternative" contentType="CDATA"><title>The ectopically parting cells 1-2 (epc1-2) mutant exhibits an exaggerated response to abscisic acid</title></titleInfo><name type="personal"><namePart type="given">Lindsay</namePart><namePart type="family">Bown</namePart><affiliation>Warwick HRI, University of Warwick, Wellesbourne, Warwick CV35 9EF, UK</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal" displayLabel="corresp"><namePart type="given">Shinnosuke</namePart><namePart type="family">Kusaba</namePart><affiliation>Warwick HRI, University of Warwick, Wellesbourne, Warwick CV35 9EF, UK</affiliation><affiliation>E-mail: stephen.jackson@warwick.ac.uk</affiliation><description>Present address: National Agricultural Research Center for Western Region 2575 Ikano, Zentsuji, Kagawa 765-0053, Japan.</description><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">Florence</namePart><namePart type="family">Goubet</namePart><affiliation>Department of Biochemistry, Downing site, Cambridge CB2 1QW, UK</affiliation><description>Present address: Bayer BioScience NV, Technologiepark 38, B-9052 Gent, Belgium.</description><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">Lesley</namePart><namePart type="family">Codrai</namePart><affiliation>Warwick HRI, University of Warwick, Wellesbourne, Warwick CV35 9EF, UK</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">Aaron G.</namePart><namePart type="family">Dale</namePart><affiliation>Department of Biochemistry, Downing site, Cambridge CB2 1QW, UK</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">Zhinong</namePart><namePart type="family">Zhang</namePart><affiliation>Department of Biochemistry, Downing site, Cambridge CB2 1QW, UK</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">Xiaolan</namePart><namePart type="family">Yu</namePart><affiliation>Department of Biochemistry, Downing site, Cambridge CB2 1QW, UK</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">Karl</namePart><namePart type="family">Morris</namePart><affiliation>Warwick HRI, University of Warwick, Wellesbourne, Warwick CV35 9EF, UK</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">Tadashi</namePart><namePart type="family">Ishii</namePart><affiliation>Forestry and Forest Products Research Institute, 1 Matunosato, Tsukuba, Ibaraki, 305-8687, Japan</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">Carol</namePart><namePart type="family">Evered</namePart><affiliation>Warwick HRI, University of Warwick, Wellesbourne, Warwick CV35 9EF, UK</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">Paul</namePart><namePart type="family">Dupree</namePart><affiliation>Department of Biochemistry, Downing site, Cambridge CB2 1QW, UK</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal" displayLabel="corresp"><namePart type="given">Stephen</namePart><namePart type="family">Jackson</namePart><affiliation>Warwick HRI, University of Warwick, Wellesbourne, Warwick CV35 9EF, UK</affiliation><affiliation>E-mail: stephen.jackson@warwick.ac.uk</affiliation><role><roleTerm type="text">author</roleTerm></role></name><typeOfResource>text</typeOfResource><genre type="research-article" displayLabel="research-article"></genre><subject><topic>Research Papers</topic></subject><originInfo><publisher>Oxford University Press</publisher><dateIssued encoding="w3cdtf">2007-04-10</dateIssued><dateCreated encoding="w3cdtf">2007-04-10</dateCreated><copyrightDate encoding="w3cdtf">2007</copyrightDate></originInfo><language><languageTerm type="code" authority="iso639-2b">eng</languageTerm><languageTerm type="code" authority="rfc3066">en</languageTerm></language><physicalDescription><internetMediaType>text/html</internetMediaType></physicalDescription><abstract>The ECTOPICALLY PARTING CELLS 1 (EPC1) gene encodes a putative retaining glycosyltransferase of the GT64 family, and epc1-1 mutant plants have a severely dwarfed phenotype. A new mutant allele of this gene, epc1-2, has been isolated. Reduced cell adhesion that has previously been reported for the epc1-1 mutant was not observed for either the epc1-1 or epc1-2 mutants grown in our conditions, suggesting that EPC1 does not affect cell adhesion but is involved in some other process affecting plant growth and development. It is shown that the epc1-2 mutant exhibits hypersensitivity to the phytohormone abscisic acid in germination and root elongation assays, however it shows an unaltered response to gibberellin, epi-brassinosteroid, auxin, or ethylene. An EPC1:YFP fusion protein is localized to small motile structures within the cytosol that are similar in size and number to the Golgi apparatus. Analysis of cell wall pectins revealed that levels of -(1,4)-galactan in the epc1-2 mutant are reduced by 50%, whilst other pectic polysaccharides (homogalacturonan, arabinan, and rhamnogalacturonan II) are unchanged.</abstract><note type="footnotes">Present address: National Agricultural Research Center for Western Region 2575 Ikano, Zentsuji, Kagawa 765-0053, Japan.</note><subject><genre>keywords</genre><topic>ABA</topic><topic>cell wall</topic><topic>dwarf</topic><topic>galactan</topic><topic>pectin</topic><topic>root hair</topic></subject><relatedItem type="host"><titleInfo><title>Journal of Experimental Botany</title></titleInfo><genre type="journal">journal</genre><identifier type="ISSN">0022-0957</identifier><identifier type="eISSN">1460-2431</identifier><identifier type="PublisherID">exbotj</identifier><identifier type="PublisherID-hwp">jexbot</identifier><part><date>2007</date><detail type="volume"><caption>vol.</caption><number>58</number></detail><detail type="issue"><caption>no.</caption><number>7</number></detail><extent unit="pages"><start>1813</start><end>1823</end></extent></part></relatedItem><identifier type="istex">AAB09354B7951E12A7302D3B2D698657CC1565B1</identifier><identifier type="DOI">10.1093/jxb/erm040</identifier><accessCondition type="use and reproduction" contentType="open-access">This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/2.0/uk/) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.</accessCondition><recordInfo><recordContentSource>OUP</recordContentSource></recordInfo></mods></metadata><covers><json:item><original>true</original><mimetype>image/tiff</mimetype><extension>tiff</extension><uri>https://api.istex.fr/document/AAB09354B7951E12A7302D3B2D698657CC1565B1/covers/tiff</uri></json:item><json:item><original>true</original><mimetype>text/html</mimetype><extension>html</extension><uri>https://api.istex.fr/document/AAB09354B7951E12A7302D3B2D698657CC1565B1/covers/html</uri></json:item></covers><annexes><json:item><original>true</original><mimetype>image/jpeg</mimetype><extension>jpeg</extension><uri>https://api.istex.fr/document/AAB09354B7951E12A7302D3B2D698657CC1565B1/annexes/jpeg</uri></json:item><json:item><original>true</original><mimetype>image/gif</mimetype><extension>gif</extension><uri>https://api.istex.fr/document/AAB09354B7951E12A7302D3B2D698657CC1565B1/annexes/gif</uri></json:item></annexes><enrichments><istex:catWosTEI uri="https://api.istex.fr/document/AAB09354B7951E12A7302D3B2D698657CC1565B1/enrichments/catWos"><teiHeader><profileDesc><textClass><classCode scheme="WOS">PLANT SCIENCES</classCode></textClass></profileDesc></teiHeader></istex:catWosTEI><json:item><type>refBibs</type><uri>https://api.istex.fr/document/AAB09354B7951E12A7302D3B2D698657CC1565B1/enrichments/refBibs</uri></json:item></enrichments><serie></serie></istex></record>Pour manipuler ce document sous Unix (Dilib)
EXPLOR_STEP=$WICRI_ROOT/Wicri/Belgique/explor/OpenAccessBelV2/Data/Istex/Corpus
HfdSelect -h $EXPLOR_STEP/biblio.hfd -nk 001488 | SxmlIndent | more
Ou
HfdSelect -h $EXPLOR_AREA/Data/Istex/Corpus/biblio.hfd -nk 001488 | SxmlIndent | more
Pour mettre un lien sur cette page dans le réseau Wicri
{{Explor lien
|wiki= Wicri/Belgique
|area= OpenAccessBelV2
|flux= Istex
|étape= Corpus
|type= RBID
|clé= ISTEX:AAB09354B7951E12A7302D3B2D698657CC1565B1
|texte= The ectopically parting cells 1-2 (epc1-2) mutant exhibits an exaggerated response to abscisic acid
}}
| This area was generated with Dilib version V0.6.25. |  |