From genome to proteome: developing expression clone resources for the human genome
Identifieur interne : 001301 ( Istex/Corpus ); précédent : 001300; suivant : 001302From genome to proteome: developing expression clone resources for the human genome
Auteurs : Gary Temple ; Philippe Lamesch ; Stuart Milstein ; David E. Hill ; Lukas Wagner ; Troy Moore ; Marc VidalSource :
- Human Molecular Genetics [ 0964-6906 ] ; 2006-04-15.
Abstract
cDNA clones have long been valuable reagents for studying the structure and function of proteins. With recent access to the entire human genome sequence, it has become possible and highly productive to compare the sequences of mRNAs to their genes, in order to validate the sequences and protein-coding annotations of each (1,2). Thus, well-characterized collections of human cDNAs are now playing an essential role in defining the structure and function of human genes and proteins. In this review, we will summarize the major collections of human cDNA clones, discuss some limitations common to most of these collections and describe several noteworthy proteomics applications, focusing on the detection and analysis of protein–protein interactions (PPI). These human cDNA collections contain principally two types of cDNA clones. The largest collections comprise cDNAs with full-length protein coding sequences (FL-CDS). Some but not all of these cDNA clones may represent the entire mRNA sequence, but many are missing considerable non-coding UTR sequence, usually at the 5′ end. A second type of cDNA clone, a ‘full-ORF’ (F-ORF) expression clone, is one where the annotated protein-coding sequence, excised of 5′ UTR and 3′ UTR sequence, has been transferred to a vector designed to facilitate transfer to other vectors for protein expression.
Url:
DOI: 10.1093/hmg/ddl048
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<front><div type="abstract" xml:lang="en">cDNA clones have long been valuable reagents for studying the structure and function of proteins. With recent access to the entire human genome sequence, it has become possible and highly productive to compare the sequences of mRNAs to their genes, in order to validate the sequences and protein-coding annotations of each (1,2). Thus, well-characterized collections of human cDNAs are now playing an essential role in defining the structure and function of human genes and proteins. In this review, we will summarize the major collections of human cDNA clones, discuss some limitations common to most of these collections and describe several noteworthy proteomics applications, focusing on the detection and analysis of protein–protein interactions (PPI). These human cDNA collections contain principally two types of cDNA clones. The largest collections comprise cDNAs with full-length protein coding sequences (FL-CDS). Some but not all of these cDNA clones may represent the entire mRNA sequence, but many are missing considerable non-coding UTR sequence, usually at the 5′ end. A second type of cDNA clone, a ‘full-ORF’ (F-ORF) expression clone, is one where the annotated protein-coding sequence, excised of 5′ UTR and 3′ UTR sequence, has been transferred to a vector designed to facilitate transfer to other vectors for protein expression.</div>
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Unité de Recherche en Biologie Moléculaire, Facultés Universitaires Notre-Dame de la Paix, 5000 Namur, Belgium and</aff>
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To whom correspondence should be addressed. Tel: +1 3015945951; Fax: +1 3014802770; Email: <ext-link xlink:href="gtemple@mail.nih.gov" ext-link-type="email">gtemple@mail.nih.gov</ext-link>
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The online version of this article has been published under an open access model. Users are entitled to use, reproduce, disseminate, or display the open access version of this article for non-commercial purposes provided that: the original authorship is properly and fully attributed; the Journal and Oxford University Press are attributed as the original place of publication with the correct citation details given; if an article is subsequently reproduced or disseminated not in its entirety but only in part or as a derivative work this must be clearly indicated. For commercial re-use, please contact: journals.permissions@oxfordjournals.org</copyright-statement>
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<abstract xml:lang="en"><p>cDNA clones have long been valuable reagents for studying the structure and function of proteins. With recent access to the entire human genome sequence, it has become possible and highly productive to compare the sequences of mRNAs to their genes, in order to validate the sequences and protein-coding annotations of each (1,2). Thus, well-characterized collections of human cDNAs are now playing an essential role in defining the structure and function of human genes and proteins. In this review, we will summarize the major collections of human cDNA clones, discuss some limitations common to most of these collections and describe several noteworthy proteomics applications, focusing on the detection and analysis of protein–protein interactions (PPI). These human cDNA collections contain principally two types of cDNA clones. The largest collections comprise cDNAs with full-length protein coding sequences (FL-CDS). Some but not all of these cDNA clones may represent the entire mRNA sequence, but many are missing considerable non-coding UTR sequence, usually at the 5′ end. A second type of cDNA clone, a ‘full-ORF’ (F-ORF) expression clone, is one where the annotated protein-coding sequence, excised of 5′ UTR and 3′ UTR sequence, has been transferred to a vector designed to facilitate transfer to other vectors for protein expression.</p>
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<name type="personal"><namePart type="given">Gary</namePart>
<namePart type="family">Temple</namePart>
<affiliation>Mammalian Gene Collection, National Human Genome Research Institute and</affiliation>
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<name type="personal"><namePart type="given">Philippe</namePart>
<namePart type="family">Lamesch</namePart>
<affiliation>Center for Cancer Systems Biology (CCSB) and Department of Cancer Biology, Dana-Farber Cancer Institute and Department of Genetics, Harvard Medical School, Boston, MA 02115, USA,</affiliation>
<affiliation>Unité de Recherche en Biologie Moléculaire, Facultés Universitaires Notre-Dame de la Paix, 5000 Namur, Belgium and</affiliation>
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<name type="personal"><namePart type="given">Stuart</namePart>
<namePart type="family">Milstein</namePart>
<affiliation>Center for Cancer Systems Biology (CCSB) and Department of Cancer Biology, Dana-Farber Cancer Institute and Department of Genetics, Harvard Medical School, Boston, MA 02115, USA,</affiliation>
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<name type="personal"><namePart type="given">David E.</namePart>
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<name type="personal"><namePart type="given">Lukas</namePart>
<namePart type="family">Wagner</namePart>
<affiliation>National Center for Biotechnology Information, National Library of Medicine, National Institutes of Health, Bethesda, MD 20892, USA,</affiliation>
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<name type="personal"><namePart type="given">Troy</namePart>
<namePart type="family">Moore</namePart>
<affiliation>Open Biosystems, Huntsville, AL 35806, USA</affiliation>
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<name type="personal"><namePart type="given">Marc</namePart>
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<abstract lang="en">cDNA clones have long been valuable reagents for studying the structure and function of proteins. With recent access to the entire human genome sequence, it has become possible and highly productive to compare the sequences of mRNAs to their genes, in order to validate the sequences and protein-coding annotations of each (1,2). Thus, well-characterized collections of human cDNAs are now playing an essential role in defining the structure and function of human genes and proteins. In this review, we will summarize the major collections of human cDNA clones, discuss some limitations common to most of these collections and describe several noteworthy proteomics applications, focusing on the detection and analysis of protein–protein interactions (PPI). These human cDNA collections contain principally two types of cDNA clones. The largest collections comprise cDNAs with full-length protein coding sequences (FL-CDS). Some but not all of these cDNA clones may represent the entire mRNA sequence, but many are missing considerable non-coding UTR sequence, usually at the 5′ end. A second type of cDNA clone, a ‘full-ORF’ (F-ORF) expression clone, is one where the annotated protein-coding sequence, excised of 5′ UTR and 3′ UTR sequence, has been transferred to a vector designed to facilitate transfer to other vectors for protein expression.</abstract>
<note type="author-notes">*To whom correspondence should be addressed. Tel: +1 3015945951; Fax: +1 3014802770; Email: gtemple@mail.nih.gov</note>
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