Quantitative detection of norovirus excretion in pediatric patients with cancer and prolonged gastroenteritis and shedding of norovirus
Identifieur interne : 000F34 ( Istex/Corpus ); précédent : 000F33; suivant : 000F35Quantitative detection of norovirus excretion in pediatric patients with cancer and prolonged gastroenteritis and shedding of norovirus
Auteurs : A. Ludwig ; O. Adams ; H. Laws ; H. Schroten ; T. TenenbaumSource :
- Journal of Medical Virology [ 0146-6615 ] ; 2008-08.
English descriptors
Abstract
Although chronic courses of norovirus infection have been described in immunocompromised patients, little is known about noroviral shedding and correlation with clinical symptoms in these patients. In this report, the quantitative courses of norovirus excretion in nine pediatric patients with hematologic and oncologic disorders and prolonged gastroenteritis were investigated. In a retrospective study multiple fecal samples from nine pediatric cancer patients were examined by a one‐step real‐time PCR. Clinical data of the patients were reviewed and virological data were correlated with clinical symptoms. All nine patients presented with prolonged illness and prolonged noroviral shedding. Vomiting and diarrhea were associated with high norovirus concentrations and norovirus excretion declined slowly in the patients. Retrospectively, initial PCR‐testing for norovirus was performed with a median of 7 days after onset of symptoms. This finding hints at the difficulty of obtaining early diagnosis of the infection in these children. The patients were shedding high norovirus concentration over a long period of time. Results of sequential quantitative PCR‐testing for norovirus correlated with clinical symptoms. Both clinical symptoms and quantitative PCR‐testings help to define the severity of norovirus infection and to estimate the risk for transmission. To prevent the spread of the disease, usage of virocidal disinfectants and isolation procedures should be maintained as long as patients are positive for noroviruses. Since vomiting is frequent in pediatric patients with oncological conditions, a screening program for rapid detection of norovirus infection in this group of patients should be considered. J. Med. Virol. 80:1461–1467, 2008. © 2008 Wiley‐Liss, Inc.
Url:
DOI: 10.1002/jmv.21217
Links to Exploration step
ISTEX:57C60BBF02576E3A52C338C410406DB4E59EE2EBLe document en format XML
<record><TEI wicri:istexFullTextTei="biblStruct"><teiHeader><fileDesc><titleStmt><title xml:lang="en">Quantitative detection of norovirus excretion in pediatric patients with cancer and prolonged gastroenteritis and shedding of norovirus</title><author><name sortKey="Ludwig, A" sort="Ludwig, A" uniqKey="Ludwig A" first="A." last="Ludwig">A. Ludwig</name><affiliation><mods:affiliation>Institute for Virology, Heinrich‐Heine‐University Duesseldorf, Duesseldorf, Germany</mods:affiliation></affiliation></author><author><name sortKey="Adams, O" sort="Adams, O" uniqKey="Adams O" first="O." last="Adams">O. Adams</name><affiliation><mods:affiliation>Institute for Virology, Heinrich‐Heine‐University Duesseldorf, Duesseldorf, Germany</mods:affiliation></affiliation></author><author><name sortKey="Laws, H" sort="Laws, H" uniqKey="Laws H" first="H." last="Laws">H. Laws</name><affiliation><mods:affiliation>Department of Pediatric Oncology, Hematology and Clinical Immunology, Heinrich‐Heine‐University Duesseldorf, Duesseldorf, Germany</mods:affiliation></affiliation></author><author><name sortKey="Schroten, H" sort="Schroten, H" uniqKey="Schroten H" first="H." last="Schroten">H. Schroten</name><affiliation><mods:affiliation>Pediatric Infectious Diseases, Department of Pediatrics, University Hospital Mannheim, Mannheim, Germany</mods:affiliation></affiliation></author><author><name sortKey="Tenenbaum, T" sort="Tenenbaum, T" uniqKey="Tenenbaum T" first="T." last="Tenenbaum">T. Tenenbaum</name><affiliation><mods:affiliation>Pediatric Infectious Diseases, Department of Pediatrics, University Hospital Mannheim, Mannheim, Germany</mods:affiliation></affiliation></author></titleStmt><publicationStmt><idno type="wicri:source">ISTEX</idno><idno type="RBID">ISTEX:57C60BBF02576E3A52C338C410406DB4E59EE2EB</idno><date when="2008" year="2008">2008</date><idno type="doi">10.1002/jmv.21217</idno><idno type="url">https://api.istex.fr/document/57C60BBF02576E3A52C338C410406DB4E59EE2EB/fulltext/pdf</idno><idno type="wicri:Area/Istex/Corpus">000F34</idno></publicationStmt><sourceDesc><biblStruct><analytic><title level="a" type="main" xml:lang="en">Quantitative detection of norovirus excretion in pediatric patients with cancer and prolonged gastroenteritis and shedding of norovirus</title><author><name sortKey="Ludwig, A" sort="Ludwig, A" uniqKey="Ludwig A" first="A." last="Ludwig">A. Ludwig</name><affiliation><mods:affiliation>Institute for Virology, Heinrich‐Heine‐University Duesseldorf, Duesseldorf, Germany</mods:affiliation></affiliation></author><author><name sortKey="Adams, O" sort="Adams, O" uniqKey="Adams O" first="O." last="Adams">O. Adams</name><affiliation><mods:affiliation>Institute for Virology, Heinrich‐Heine‐University Duesseldorf, Duesseldorf, Germany</mods:affiliation></affiliation></author><author><name sortKey="Laws, H" sort="Laws, H" uniqKey="Laws H" first="H." last="Laws">H. Laws</name><affiliation><mods:affiliation>Department of Pediatric Oncology, Hematology and Clinical Immunology, Heinrich‐Heine‐University Duesseldorf, Duesseldorf, Germany</mods:affiliation></affiliation></author><author><name sortKey="Schroten, H" sort="Schroten, H" uniqKey="Schroten H" first="H." last="Schroten">H. Schroten</name><affiliation><mods:affiliation>Pediatric Infectious Diseases, Department of Pediatrics, University Hospital Mannheim, Mannheim, Germany</mods:affiliation></affiliation></author><author><name sortKey="Tenenbaum, T" sort="Tenenbaum, T" uniqKey="Tenenbaum T" first="T." last="Tenenbaum">T. Tenenbaum</name><affiliation><mods:affiliation>Pediatric Infectious Diseases, Department of Pediatrics, University Hospital Mannheim, Mannheim, Germany</mods:affiliation></affiliation></author></analytic><monogr></monogr><series><title level="j">Journal of Medical Virology</title><title level="j" type="abbrev">J. Med. Virol.</title><idno type="ISSN">0146-6615</idno><idno type="eISSN">1096-9071</idno><imprint><publisher>Wiley Subscription Services, Inc., A Wiley Company</publisher><pubPlace>Hoboken</pubPlace><date type="published" when="2008-08">2008-08</date><biblScope unit="volume">80</biblScope><biblScope unit="issue">8</biblScope><biblScope unit="page" from="1461">1461</biblScope><biblScope unit="page" to="1467">1467</biblScope></imprint><idno type="ISSN">0146-6615</idno></series><idno type="istex">57C60BBF02576E3A52C338C410406DB4E59EE2EB</idno><idno type="DOI">10.1002/jmv.21217</idno><idno type="ArticleID">JMV21217</idno></biblStruct></sourceDesc><seriesStmt><idno type="ISSN">0146-6615</idno></seriesStmt></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>immunocompromised patients</term><term>norovirus</term><term>quantitative real‐time PCR</term></keywords></textClass><langUsage><language ident="en">en</language></langUsage></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Although chronic courses of norovirus infection have been described in immunocompromised patients, little is known about noroviral shedding and correlation with clinical symptoms in these patients. In this report, the quantitative courses of norovirus excretion in nine pediatric patients with hematologic and oncologic disorders and prolonged gastroenteritis were investigated. In a retrospective study multiple fecal samples from nine pediatric cancer patients were examined by a one‐step real‐time PCR. Clinical data of the patients were reviewed and virological data were correlated with clinical symptoms. All nine patients presented with prolonged illness and prolonged noroviral shedding. Vomiting and diarrhea were associated with high norovirus concentrations and norovirus excretion declined slowly in the patients. Retrospectively, initial PCR‐testing for norovirus was performed with a median of 7 days after onset of symptoms. This finding hints at the difficulty of obtaining early diagnosis of the infection in these children. The patients were shedding high norovirus concentration over a long period of time. Results of sequential quantitative PCR‐testing for norovirus correlated with clinical symptoms. Both clinical symptoms and quantitative PCR‐testings help to define the severity of norovirus infection and to estimate the risk for transmission. To prevent the spread of the disease, usage of virocidal disinfectants and isolation procedures should be maintained as long as patients are positive for noroviruses. Since vomiting is frequent in pediatric patients with oncological conditions, a screening program for rapid detection of norovirus infection in this group of patients should be considered. J. Med. Virol. 80:1461–1467, 2008. © 2008 Wiley‐Liss, Inc.</div></front></TEI><istex><corpusName>wiley</corpusName><author><json:item><name>A. Ludwig</name><affiliations><json:string>Institute for Virology, Heinrich‐Heine‐University Duesseldorf, Duesseldorf, Germany</json:string></affiliations></json:item><json:item><name>O. Adams</name><affiliations><json:string>Institute for Virology, Heinrich‐Heine‐University Duesseldorf, Duesseldorf, Germany</json:string></affiliations></json:item><json:item><name>H.‐J. Laws</name><affiliations><json:string>Department of Pediatric Oncology, Hematology and Clinical Immunology, Heinrich‐Heine‐University Duesseldorf, Duesseldorf, Germany</json:string></affiliations></json:item><json:item><name>H. Schroten</name><affiliations><json:string>Pediatric Infectious Diseases, Department of Pediatrics, University Hospital Mannheim, Mannheim, Germany</json:string></affiliations></json:item><json:item><name>T. Tenenbaum</name><affiliations><json:string>Pediatric Infectious Diseases, Department of Pediatrics, University Hospital Mannheim, Mannheim, Germany</json:string></affiliations></json:item></author><subject><json:item><lang><json:string>eng</json:string></lang><value>norovirus</value></json:item><json:item><lang><json:string>eng</json:string></lang><value>immunocompromised patients</value></json:item><json:item><lang><json:string>eng</json:string></lang><value>quantitative real‐time PCR</value></json:item></subject><articleId><json:string>JMV21217</json:string></articleId><language><json:string>eng</json:string></language><originalGenre><json:string>article</json:string></originalGenre><abstract>Although chronic courses of norovirus infection have been described in immunocompromised patients, little is known about noroviral shedding and correlation with clinical symptoms in these patients. In this report, the quantitative courses of norovirus excretion in nine pediatric patients with hematologic and oncologic disorders and prolonged gastroenteritis were investigated. In a retrospective study multiple fecal samples from nine pediatric cancer patients were examined by a one‐step real‐time PCR. Clinical data of the patients were reviewed and virological data were correlated with clinical symptoms. All nine patients presented with prolonged illness and prolonged noroviral shedding. Vomiting and diarrhea were associated with high norovirus concentrations and norovirus excretion declined slowly in the patients. Retrospectively, initial PCR‐testing for norovirus was performed with a median of 7 days after onset of symptoms. This finding hints at the difficulty of obtaining early diagnosis of the infection in these children. The patients were shedding high norovirus concentration over a long period of time. Results of sequential quantitative PCR‐testing for norovirus correlated with clinical symptoms. Both clinical symptoms and quantitative PCR‐testings help to define the severity of norovirus infection and to estimate the risk for transmission. To prevent the spread of the disease, usage of virocidal disinfectants and isolation procedures should be maintained as long as patients are positive for noroviruses. Since vomiting is frequent in pediatric patients with oncological conditions, a screening program for rapid detection of norovirus infection in this group of patients should be considered. J. Med. Virol. 80:1461–1467, 2008. © 2008 Wiley‐Liss, Inc.</abstract><qualityIndicators><score>7.309</score><pdfVersion>1.3</pdfVersion><pdfPageSize>594 x 792 pts</pdfPageSize><refBibsNative>true</refBibsNative><keywordCount>3</keywordCount><abstractCharCount>1783</abstractCharCount><pdfWordCount>4309</pdfWordCount><pdfCharCount>28277</pdfCharCount><pdfPageCount>7</pdfPageCount><abstractWordCount>250</abstractWordCount></qualityIndicators><title>Quantitative detection of norovirus excretion in pediatric patients with cancer and prolonged gastroenteritis and shedding of norovirus</title><genre><json:string>article</json:string></genre><host><volume>80</volume><publisherId><json:string>JMV</json:string></publisherId><pages><total>7</total><last>1467</last><first>1461</first></pages><issn><json:string>0146-6615</json:string></issn><issue>8</issue><subject><json:item><value>Research Article</value></json:item></subject><genre><json:string>journal</json:string></genre><language><json:string>unknown</json:string></language><eissn><json:string>1096-9071</json:string></eissn><title>Journal of Medical Virology</title><doi><json:string>10.1002/(ISSN)1096-9071</json:string></doi></host><publicationDate>2008</publicationDate><copyrightDate>2008</copyrightDate><doi><json:string>10.1002/jmv.21217</json:string></doi><id>57C60BBF02576E3A52C338C410406DB4E59EE2EB</id><score>0.2441903</score><fulltext><json:item><original>true</original><mimetype>application/pdf</mimetype><extension>pdf</extension><uri>https://api.istex.fr/document/57C60BBF02576E3A52C338C410406DB4E59EE2EB/fulltext/pdf</uri></json:item><json:item><original>false</original><mimetype>application/zip</mimetype><extension>zip</extension><uri>https://api.istex.fr/document/57C60BBF02576E3A52C338C410406DB4E59EE2EB/fulltext/zip</uri></json:item><istex:fulltextTEI uri="https://api.istex.fr/document/57C60BBF02576E3A52C338C410406DB4E59EE2EB/fulltext/tei"><teiHeader><fileDesc><titleStmt><title level="a" type="main" xml:lang="en">Quantitative detection of norovirus excretion in pediatric patients with cancer and prolonged gastroenteritis and shedding of norovirus</title></titleStmt><publicationStmt><authority>ISTEX</authority><publisher>Wiley Subscription Services, Inc., A Wiley Company</publisher><pubPlace>Hoboken</pubPlace><availability><p>Copyright © 2008 Wiley‐Liss, Inc.</p></availability><date>2008</date></publicationStmt><sourceDesc><biblStruct type="inbook"><analytic><title level="a" type="main" xml:lang="en">Quantitative detection of norovirus excretion in pediatric patients with cancer and prolonged gastroenteritis and shedding of norovirus</title><author xml:id="author-1"><persName><forename type="first">A.</forename><surname>Ludwig</surname></persName><note type="correspondence"><p>Correspondence: Institute for Virology, Geb. 22.21, Universitaetsstr. 1, D‐40225 Duesseldorf, Germany.===</p></note><affiliation>Institute for Virology, Heinrich‐Heine‐University Duesseldorf, Duesseldorf, Germany</affiliation></author><author xml:id="author-2"><persName><forename type="first">O.</forename><surname>Adams</surname></persName><affiliation>Institute for Virology, Heinrich‐Heine‐University Duesseldorf, Duesseldorf, Germany</affiliation></author><author xml:id="author-3"><persName><forename type="first">H.‐J.</forename><surname>Laws</surname></persName><affiliation>Department of Pediatric Oncology, Hematology and Clinical Immunology, Heinrich‐Heine‐University Duesseldorf, Duesseldorf, Germany</affiliation></author><author xml:id="author-4"><persName><forename type="first">H.</forename><surname>Schroten</surname></persName><affiliation>Pediatric Infectious Diseases, Department of Pediatrics, University Hospital Mannheim, Mannheim, Germany</affiliation></author><author xml:id="author-5"><persName><forename type="first">T.</forename><surname>Tenenbaum</surname></persName><affiliation>Pediatric Infectious Diseases, Department of Pediatrics, University Hospital Mannheim, Mannheim, Germany</affiliation></author></analytic><monogr><title level="j">Journal of Medical Virology</title><title level="j" type="abbrev">J. Med. Virol.</title><idno type="pISSN">0146-6615</idno><idno type="eISSN">1096-9071</idno><idno type="DOI">10.1002/(ISSN)1096-9071</idno><imprint><publisher>Wiley Subscription Services, Inc., A Wiley Company</publisher><pubPlace>Hoboken</pubPlace><date type="published" when="2008-08"></date><biblScope unit="volume">80</biblScope><biblScope unit="issue">8</biblScope><biblScope unit="page" from="1461">1461</biblScope><biblScope unit="page" to="1467">1467</biblScope></imprint></monogr><idno type="istex">57C60BBF02576E3A52C338C410406DB4E59EE2EB</idno><idno type="DOI">10.1002/jmv.21217</idno><idno type="ArticleID">JMV21217</idno></biblStruct></sourceDesc></fileDesc><profileDesc><creation><date>2008</date></creation><langUsage><language ident="en">en</language></langUsage><abstract xml:lang="en"><p>Although chronic courses of norovirus infection have been described in immunocompromised patients, little is known about noroviral shedding and correlation with clinical symptoms in these patients. In this report, the quantitative courses of norovirus excretion in nine pediatric patients with hematologic and oncologic disorders and prolonged gastroenteritis were investigated. In a retrospective study multiple fecal samples from nine pediatric cancer patients were examined by a one‐step real‐time PCR. Clinical data of the patients were reviewed and virological data were correlated with clinical symptoms. All nine patients presented with prolonged illness and prolonged noroviral shedding. Vomiting and diarrhea were associated with high norovirus concentrations and norovirus excretion declined slowly in the patients. Retrospectively, initial PCR‐testing for norovirus was performed with a median of 7 days after onset of symptoms. This finding hints at the difficulty of obtaining early diagnosis of the infection in these children. The patients were shedding high norovirus concentration over a long period of time. Results of sequential quantitative PCR‐testing for norovirus correlated with clinical symptoms. Both clinical symptoms and quantitative PCR‐testings help to define the severity of norovirus infection and to estimate the risk for transmission. To prevent the spread of the disease, usage of virocidal disinfectants and isolation procedures should be maintained as long as patients are positive for noroviruses. Since vomiting is frequent in pediatric patients with oncological conditions, a screening program for rapid detection of norovirus infection in this group of patients should be considered. J. Med. Virol. 80:1461–1467, 2008. © 2008 Wiley‐Liss, Inc.</p></abstract><textClass xml:lang="en"><keywords scheme="keyword"><list><head>keywords</head><item><term>norovirus</term></item><item><term>immunocompromised patients</term></item><item><term>quantitative real‐time PCR</term></item></list></keywords></textClass><textClass><keywords scheme="Journal Subject"><list><head>article-category</head><item><term>Research Article</term></item></list></keywords></textClass></profileDesc><revisionDesc><change when="2008-04-01">Registration</change><change when="2008-08">Published</change></revisionDesc></teiHeader></istex:fulltextTEI><json:item><original>false</original><mimetype>text/plain</mimetype><extension>txt</extension><uri>https://api.istex.fr/document/57C60BBF02576E3A52C338C410406DB4E59EE2EB/fulltext/txt</uri></json:item></fulltext><metadata><istex:metadataXml wicri:clean="Wiley, elements deleted: body"><istex:xmlDeclaration>version="1.0" encoding="UTF-8" standalone="yes"</istex:xmlDeclaration><istex:document><component version="2.0" type="serialArticle" xml:lang="en"><header><publicationMeta level="product"><publisherInfo><publisherName>Wiley Subscription Services, Inc., A Wiley Company</publisherName><publisherLoc>Hoboken</publisherLoc></publisherInfo><doi registered="yes">10.1002/(ISSN)1096-9071</doi><issn type="print">0146-6615</issn><issn type="electronic">1096-9071</issn><idGroup><id type="product" value="JMV"></id></idGroup><titleGroup><title type="main" xml:lang="en" sort="JOURNAL OF MEDICAL VIROLOGY">Journal of Medical Virology</title><title type="short">J. Med. Virol.</title></titleGroup></publicationMeta><publicationMeta level="part" position="80"><doi origin="wiley" registered="yes">10.1002/jmv.v80:8</doi><numberingGroup><numbering type="journalVolume" number="80">80</numbering><numbering type="journalIssue">8</numbering></numberingGroup><coverDate startDate="2008-08">August 2008</coverDate></publicationMeta><publicationMeta level="unit" type="article" position="210" status="forIssue"><doi origin="wiley" registered="yes">10.1002/jmv.21217</doi><idGroup><id type="unit" value="JMV21217"></id></idGroup><countGroup><count type="pageTotal" number="7"></count></countGroup><titleGroup><title type="articleCategory">Research Article</title></titleGroup><copyright ownership="publisher">Copyright © 2008 Wiley‐Liss, Inc.</copyright><eventGroup><event type="manuscriptAccepted" date="2008-04-01"></event><event type="firstOnline" date="2008-06-12"></event><event type="publishedOnlineFinalForm" date="2008-06-12"></event><event type="xmlConverted" agent="Converter:JWSART34_TO_WML3G version:2.3.2 mode:FullText source:HeaderRef result:HeaderRef" date="2010-03-15"></event><event type="xmlConverted" agent="Converter:WILEY_ML3G_TO_WILEY_ML3GV2 version:4.0.1" date="2014-03-20"></event><event type="xmlConverted" agent="Converter:WML3G_To_WML3G version:4.1.7 mode:FullText,remove_FC" date="2014-10-30"></event></eventGroup><numberingGroup><numbering type="pageFirst">1461</numbering><numbering type="pageLast">1467</numbering></numberingGroup><correspondenceTo>Institute for Virology, Geb. 22.21, Universitaetsstr. 1, D‐40225 Duesseldorf, Germany.===</correspondenceTo><linkGroup><link type="toTypesetVersion" href="file:JMV.JMV21217.pdf"></link></linkGroup></publicationMeta><contentMeta><countGroup><count type="figureTotal" number="2"></count><count type="tableTotal" number="2"></count><count type="referenceTotal" number="21"></count><count type="wordTotal" number="878"></count></countGroup><titleGroup><title type="main" xml:lang="en">Quantitative detection of norovirus excretion in pediatric patients with cancer and prolonged gastroenteritis and shedding of norovirus</title><title type="short" xml:lang="en">Norovirus Infections in Pediatric Patients With Cancer</title></titleGroup><creators><creator xml:id="au1" creatorRole="author" affiliationRef="#af1" corresponding="yes"><personName><givenNames>A.</givenNames><familyName>Ludwig</familyName></personName><contactDetails><email normalForm="amei.ludwig@uni-duesseldorf.de">amei.ludwig@uni‐duesseldorf.de</email></contactDetails></creator><creator xml:id="au2" creatorRole="author" affiliationRef="#af1"><personName><givenNames>O.</givenNames><familyName>Adams</familyName></personName></creator><creator xml:id="au3" creatorRole="author" affiliationRef="#af2"><personName><givenNames>H.‐J.</givenNames><familyName>Laws</familyName></personName></creator><creator xml:id="au4" creatorRole="author" affiliationRef="#af3"><personName><givenNames>H.</givenNames><familyName>Schroten</familyName></personName></creator><creator xml:id="au5" creatorRole="author" affiliationRef="#af3"><personName><givenNames>T.</givenNames><familyName>Tenenbaum</familyName></personName></creator></creators><affiliationGroup><affiliation xml:id="af1" countryCode="DE" type="organization"><unparsedAffiliation>Institute for Virology, Heinrich‐Heine‐University Duesseldorf, Duesseldorf, Germany</unparsedAffiliation></affiliation><affiliation xml:id="af2" countryCode="DE" type="organization"><unparsedAffiliation>Department of Pediatric Oncology, Hematology and Clinical Immunology, Heinrich‐Heine‐University Duesseldorf, Duesseldorf, Germany</unparsedAffiliation></affiliation><affiliation xml:id="af3" countryCode="DE" type="organization"><unparsedAffiliation>Pediatric Infectious Diseases, Department of Pediatrics, University Hospital Mannheim, Mannheim, Germany</unparsedAffiliation></affiliation></affiliationGroup><keywordGroup xml:lang="en" type="author"><keyword xml:id="kwd1">norovirus</keyword><keyword xml:id="kwd2">immunocompromised patients</keyword><keyword xml:id="kwd3">quantitative real‐time PCR</keyword></keywordGroup><abstractGroup><abstract type="main" xml:lang="en"><title type="main">Abstract</title><p>Although chronic courses of norovirus infection have been described in immunocompromised patients, little is known about noroviral shedding and correlation with clinical symptoms in these patients. In this report, the quantitative courses of norovirus excretion in nine pediatric patients with hematologic and oncologic disorders and prolonged gastroenteritis were investigated. In a retrospective study multiple fecal samples from nine pediatric cancer patients were examined by a one‐step real‐time PCR. Clinical data of the patients were reviewed and virological data were correlated with clinical symptoms. All nine patients presented with prolonged illness and prolonged noroviral shedding. Vomiting and diarrhea were associated with high norovirus concentrations and norovirus excretion declined slowly in the patients. Retrospectively, initial PCR‐testing for norovirus was performed with a median of 7 days after onset of symptoms. This finding hints at the difficulty of obtaining early diagnosis of the infection in these children. The patients were shedding high norovirus concentration over a long period of time. Results of sequential quantitative PCR‐testing for norovirus correlated with clinical symptoms. Both clinical symptoms and quantitative PCR‐testings help to define the severity of norovirus infection and to estimate the risk for transmission. To prevent the spread of the disease, usage of virocidal disinfectants and isolation procedures should be maintained as long as patients are positive for noroviruses. Since vomiting is frequent in pediatric patients with oncological conditions, a screening program for rapid detection of norovirus infection in this group of patients should be considered. J. Med. Virol. 80:1461–1467, 2008. © 2008 Wiley‐Liss, Inc.</p></abstract></abstractGroup></contentMeta></header></component></istex:document></istex:metadataXml><mods version="3.6"><titleInfo lang="en"><title>Quantitative detection of norovirus excretion in pediatric patients with cancer and prolonged gastroenteritis and shedding of norovirus</title></titleInfo><titleInfo type="abbreviated" lang="en"><title>Norovirus Infections in Pediatric Patients With Cancer</title></titleInfo><titleInfo type="alternative" contentType="CDATA" lang="en"><title>Quantitative detection of norovirus excretion in pediatric patients with cancer and prolonged gastroenteritis and shedding of norovirus</title></titleInfo><name type="personal"><namePart type="given">A.</namePart><namePart type="family">Ludwig</namePart><affiliation>Institute for Virology, Heinrich‐Heine‐University Duesseldorf, Duesseldorf, Germany</affiliation><description>Correspondence: Institute for Virology, Geb. 22.21, Universitaetsstr. 1, D‐40225 Duesseldorf, Germany.===</description><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">O.</namePart><namePart type="family">Adams</namePart><affiliation>Institute for Virology, Heinrich‐Heine‐University Duesseldorf, Duesseldorf, Germany</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">H.‐J.</namePart><namePart type="family">Laws</namePart><affiliation>Department of Pediatric Oncology, Hematology and Clinical Immunology, Heinrich‐Heine‐University Duesseldorf, Duesseldorf, Germany</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">H.</namePart><namePart type="family">Schroten</namePart><affiliation>Pediatric Infectious Diseases, Department of Pediatrics, University Hospital Mannheim, Mannheim, Germany</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">T.</namePart><namePart type="family">Tenenbaum</namePart><affiliation>Pediatric Infectious Diseases, Department of Pediatrics, University Hospital Mannheim, Mannheim, Germany</affiliation><role><roleTerm type="text">author</roleTerm></role></name><typeOfResource>text</typeOfResource><genre type="article" displayLabel="article"></genre><originInfo><publisher>Wiley Subscription Services, Inc., A Wiley Company</publisher><place><placeTerm type="text">Hoboken</placeTerm></place><dateIssued encoding="w3cdtf">2008-08</dateIssued><dateValid encoding="w3cdtf">2008-04-01</dateValid><copyrightDate encoding="w3cdtf">2008</copyrightDate></originInfo><language><languageTerm type="code" authority="rfc3066">en</languageTerm><languageTerm type="code" authority="iso639-2b">eng</languageTerm></language><physicalDescription><internetMediaType>text/html</internetMediaType><extent unit="figures">2</extent><extent unit="tables">2</extent><extent unit="references">21</extent><extent unit="words">878</extent></physicalDescription><abstract lang="en">Although chronic courses of norovirus infection have been described in immunocompromised patients, little is known about noroviral shedding and correlation with clinical symptoms in these patients. In this report, the quantitative courses of norovirus excretion in nine pediatric patients with hematologic and oncologic disorders and prolonged gastroenteritis were investigated. In a retrospective study multiple fecal samples from nine pediatric cancer patients were examined by a one‐step real‐time PCR. Clinical data of the patients were reviewed and virological data were correlated with clinical symptoms. All nine patients presented with prolonged illness and prolonged noroviral shedding. Vomiting and diarrhea were associated with high norovirus concentrations and norovirus excretion declined slowly in the patients. Retrospectively, initial PCR‐testing for norovirus was performed with a median of 7 days after onset of symptoms. This finding hints at the difficulty of obtaining early diagnosis of the infection in these children. The patients were shedding high norovirus concentration over a long period of time. Results of sequential quantitative PCR‐testing for norovirus correlated with clinical symptoms. Both clinical symptoms and quantitative PCR‐testings help to define the severity of norovirus infection and to estimate the risk for transmission. To prevent the spread of the disease, usage of virocidal disinfectants and isolation procedures should be maintained as long as patients are positive for noroviruses. Since vomiting is frequent in pediatric patients with oncological conditions, a screening program for rapid detection of norovirus infection in this group of patients should be considered. J. Med. Virol. 80:1461–1467, 2008. © 2008 Wiley‐Liss, Inc.</abstract><subject lang="en"><genre>keywords</genre><topic>norovirus</topic><topic>immunocompromised patients</topic><topic>quantitative real‐time PCR</topic></subject><relatedItem type="host"><titleInfo><title>Journal of Medical Virology</title></titleInfo><titleInfo type="abbreviated"><title>J. Med. Virol.</title></titleInfo><genre type="journal">journal</genre><subject><genre>article-category</genre><topic>Research Article</topic></subject><identifier type="ISSN">0146-6615</identifier><identifier type="eISSN">1096-9071</identifier><identifier type="DOI">10.1002/(ISSN)1096-9071</identifier><identifier type="PublisherID">JMV</identifier><part><date>2008</date><detail type="volume"><caption>vol.</caption><number>80</number></detail><detail type="issue"><caption>no.</caption><number>8</number></detail><extent unit="pages"><start>1461</start><end>1467</end><total>7</total></extent></part></relatedItem><identifier type="istex">57C60BBF02576E3A52C338C410406DB4E59EE2EB</identifier><identifier type="DOI">10.1002/jmv.21217</identifier><identifier type="ArticleID">JMV21217</identifier><accessCondition type="use and reproduction" contentType="copyright">Copyright © 2008 Wiley‐Liss, Inc.</accessCondition><recordInfo><recordContentSource>WILEY</recordContentSource><recordOrigin>Wiley Subscription Services, Inc., A Wiley Company</recordOrigin></recordInfo></mods></metadata><enrichments><json:item><type>multicat</type><uri>https://api.istex.fr/document/57C60BBF02576E3A52C338C410406DB4E59EE2EB/enrichments/multicat</uri></json:item></enrichments><serie></serie></istex></record>Pour manipuler ce document sous Unix (Dilib)
EXPLOR_STEP=$WICRI_ROOT/Wicri/Belgique/explor/OpenAccessBelV2/Data/Istex/Corpus
HfdSelect -h $EXPLOR_STEP/biblio.hfd -nk 000F34 | SxmlIndent | more
Ou
HfdSelect -h $EXPLOR_AREA/Data/Istex/Corpus/biblio.hfd -nk 000F34 | SxmlIndent | more
Pour mettre un lien sur cette page dans le réseau Wicri
{{Explor lien
|wiki= Wicri/Belgique
|area= OpenAccessBelV2
|flux= Istex
|étape= Corpus
|type= RBID
|clé= ISTEX:57C60BBF02576E3A52C338C410406DB4E59EE2EB
|texte= Quantitative detection of norovirus excretion in pediatric patients with cancer and prolonged gastroenteritis and shedding of norovirus
}}
| This area was generated with Dilib version V0.6.25. |  |