Kinetics of tetramolecular quadruplexes
Identifieur interne : 000F03 ( Istex/Corpus ); précédent : 000F02; suivant : 000F04Kinetics of tetramolecular quadruplexes
Auteurs : Jean-Louis Mergny ; Anne De Cian ; Amar Ghelab ; Barbara Sacca ; Laurent LacroixSource :
- Nucleic Acids Research [ 0305-1048 ] ; 2005.
Abstract
The melting of tetramolecular DNA or RNA quadruplexes is kinetically irreversible. However, rather than being a hindrance, this kinetic inertia allows us to study association and dissociation processes independently. From a kinetic point of view, the association reaction is fourth order in monomer and the dissociation first order in quadruplex. The association rate constant k on, expressed in M−3·s−1 decreases with increasing temperature, reflecting a negative activation energy (E on) for the sequences presented here. Association is favored by an increase in monocation concentration. The first-order dissociation process is temperature dependent, with a very positive activation energy E off, but nearly ionic strength independent. General rules may be drawn up for various DNA and RNA sequence motifs, involving 3–6 consecutive guanines and 0–5 protruding bases. RNA quadruplexes are more stable than their DNA counterparts as a result of both faster association and slower dissociation. In most cases, no dissociation is found for G-tracts of 5 guanines or more in sodium, 4 guanines or more in potassium. The data collected here allow us to predict the amount of time required for 50% (or 90%) quadruplex formation as a function of strand sequence and concentration, temperature and ionic strength.
Url:
DOI: 10.1093/nar/gki148
Links to Exploration step
ISTEX:ACB09B8BED86DAF85CD2EA98E69A35F8744F379CLe document en format XML
<record><TEI wicri:istexFullTextTei="biblStruct"><teiHeader><fileDesc><titleStmt><title xml:lang="en">Kinetics of tetramolecular quadruplexes</title><author><name sortKey="Mergny, Jean Louis" sort="Mergny, Jean Louis" uniqKey="Mergny J" first="Jean-Louis" last="Mergny">Jean-Louis Mergny</name><affiliation><mods:affiliation>Laboratoire de Biophysique, Muséum National d'Histoire Naturelle USM503 INSERM U565, CNRS UMR 5153, 43 rue Cuvier, 75231 Paris Cedex 05, France</mods:affiliation></affiliation></author><author><name sortKey="De Cian, Anne" sort="De Cian, Anne" uniqKey="De Cian A" first="Anne" last="De Cian">Anne De Cian</name><affiliation><mods:affiliation>Laboratoire de Biophysique, Muséum National d'Histoire Naturelle USM503 INSERM U565, CNRS UMR 5153, 43 rue Cuvier, 75231 Paris Cedex 05, France</mods:affiliation></affiliation></author><author><name sortKey="Ghelab, Amar" sort="Ghelab, Amar" uniqKey="Ghelab A" first="Amar" last="Ghelab">Amar Ghelab</name><affiliation><mods:affiliation>Laboratoire de Biophysique, Muséum National d'Histoire Naturelle USM503 INSERM U565, CNRS UMR 5153, 43 rue Cuvier, 75231 Paris Cedex 05, France</mods:affiliation></affiliation></author><author><name sortKey="Sacca, Barbara" sort="Sacca, Barbara" uniqKey="Sacca B" first="Barbara" last="Sacca">Barbara Sacca</name><affiliation><mods:affiliation>Laboratoire de Biophysique, Muséum National d'Histoire Naturelle USM503 INSERM U565, CNRS UMR 5153, 43 rue Cuvier, 75231 Paris Cedex 05, France</mods:affiliation></affiliation></author><author><name sortKey="Lacroix, Laurent" sort="Lacroix, Laurent" uniqKey="Lacroix L" first="Laurent" last="Lacroix">Laurent Lacroix</name><affiliation><mods:affiliation>Laboratoire de Biophysique, Muséum National d'Histoire Naturelle USM503 INSERM U565, CNRS UMR 5153, 43 rue Cuvier, 75231 Paris Cedex 05, France</mods:affiliation></affiliation></author></titleStmt><publicationStmt><idno type="wicri:source">ISTEX</idno><idno type="RBID">ISTEX:ACB09B8BED86DAF85CD2EA98E69A35F8744F379C</idno><date when="2005" year="2005">2005</date><idno type="doi">10.1093/nar/gki148</idno><idno type="url">https://api.istex.fr/document/ACB09B8BED86DAF85CD2EA98E69A35F8744F379C/fulltext/pdf</idno><idno type="wicri:Area/Istex/Corpus">000F03</idno></publicationStmt><sourceDesc><biblStruct><analytic><title level="a" type="main" xml:lang="en">Kinetics of tetramolecular quadruplexes</title><author><name sortKey="Mergny, Jean Louis" sort="Mergny, Jean Louis" uniqKey="Mergny J" first="Jean-Louis" last="Mergny">Jean-Louis Mergny</name><affiliation><mods:affiliation>Laboratoire de Biophysique, Muséum National d'Histoire Naturelle USM503 INSERM U565, CNRS UMR 5153, 43 rue Cuvier, 75231 Paris Cedex 05, France</mods:affiliation></affiliation></author><author><name sortKey="De Cian, Anne" sort="De Cian, Anne" uniqKey="De Cian A" first="Anne" last="De Cian">Anne De Cian</name><affiliation><mods:affiliation>Laboratoire de Biophysique, Muséum National d'Histoire Naturelle USM503 INSERM U565, CNRS UMR 5153, 43 rue Cuvier, 75231 Paris Cedex 05, France</mods:affiliation></affiliation></author><author><name sortKey="Ghelab, Amar" sort="Ghelab, Amar" uniqKey="Ghelab A" first="Amar" last="Ghelab">Amar Ghelab</name><affiliation><mods:affiliation>Laboratoire de Biophysique, Muséum National d'Histoire Naturelle USM503 INSERM U565, CNRS UMR 5153, 43 rue Cuvier, 75231 Paris Cedex 05, France</mods:affiliation></affiliation></author><author><name sortKey="Sacca, Barbara" sort="Sacca, Barbara" uniqKey="Sacca B" first="Barbara" last="Sacca">Barbara Sacca</name><affiliation><mods:affiliation>Laboratoire de Biophysique, Muséum National d'Histoire Naturelle USM503 INSERM U565, CNRS UMR 5153, 43 rue Cuvier, 75231 Paris Cedex 05, France</mods:affiliation></affiliation></author><author><name sortKey="Lacroix, Laurent" sort="Lacroix, Laurent" uniqKey="Lacroix L" first="Laurent" last="Lacroix">Laurent Lacroix</name><affiliation><mods:affiliation>Laboratoire de Biophysique, Muséum National d'Histoire Naturelle USM503 INSERM U565, CNRS UMR 5153, 43 rue Cuvier, 75231 Paris Cedex 05, France</mods:affiliation></affiliation></author></analytic><monogr></monogr><series><title level="j">Nucleic Acids Research</title><title level="j" type="abbrev">Nucl. Acids Res.</title><idno type="ISSN">0305-1048</idno><idno type="eISSN">1362-4962</idno><imprint><publisher>Oxford University Press</publisher><date type="published" when="2005">2005</date><biblScope unit="volume">33</biblScope><biblScope unit="issue">1</biblScope><biblScope unit="page" from="81">81</biblScope><biblScope unit="page" to="94">94</biblScope></imprint><idno type="ISSN">0305-1048</idno></series><idno type="istex">ACB09B8BED86DAF85CD2EA98E69A35F8744F379C</idno><idno type="DOI">10.1093/nar/gki148</idno><idno type="local">gki148</idno></biblStruct></sourceDesc><seriesStmt><idno type="ISSN">0305-1048</idno></seriesStmt></fileDesc><profileDesc><textClass></textClass><langUsage><language ident="en">en</language></langUsage></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">The melting of tetramolecular DNA or RNA quadruplexes is kinetically irreversible. However, rather than being a hindrance, this kinetic inertia allows us to study association and dissociation processes independently. From a kinetic point of view, the association reaction is fourth order in monomer and the dissociation first order in quadruplex. The association rate constant k on, expressed in M−3·s−1 decreases with increasing temperature, reflecting a negative activation energy (E on) for the sequences presented here. Association is favored by an increase in monocation concentration. The first-order dissociation process is temperature dependent, with a very positive activation energy E off, but nearly ionic strength independent. General rules may be drawn up for various DNA and RNA sequence motifs, involving 3–6 consecutive guanines and 0–5 protruding bases. RNA quadruplexes are more stable than their DNA counterparts as a result of both faster association and slower dissociation. In most cases, no dissociation is found for G-tracts of 5 guanines or more in sodium, 4 guanines or more in potassium. The data collected here allow us to predict the amount of time required for 50% (or 90%) quadruplex formation as a function of strand sequence and concentration, temperature and ionic strength.</div></front></TEI><istex><corpusName>oup</corpusName><author><json:item><name>Jean-Louis Mergny</name><affiliations><json:string>Laboratoire de Biophysique, Muséum National d'Histoire Naturelle USM503 INSERM U565, CNRS UMR 5153, 43 rue Cuvier, 75231 Paris Cedex 05, France</json:string><json:string>E-mail: mergny@vnumail.com</json:string></affiliations></json:item><json:item><name>Anne De Cian</name><affiliations><json:string>Laboratoire de Biophysique, Muséum National d'Histoire Naturelle USM503 INSERM U565, CNRS UMR 5153, 43 rue Cuvier, 75231 Paris Cedex 05, France</json:string></affiliations></json:item><json:item><name>Amar Ghelab</name><affiliations><json:string>Laboratoire de Biophysique, Muséum National d'Histoire Naturelle USM503 INSERM U565, CNRS UMR 5153, 43 rue Cuvier, 75231 Paris Cedex 05, France</json:string></affiliations></json:item><json:item><name>Barbara Saccà</name><affiliations><json:string>Laboratoire de Biophysique, Muséum National d'Histoire Naturelle USM503 INSERM U565, CNRS UMR 5153, 43 rue Cuvier, 75231 Paris Cedex 05, France</json:string></affiliations></json:item><json:item><name>Laurent Lacroix</name><affiliations><json:string>Laboratoire de Biophysique, Muséum National d'Histoire Naturelle USM503 INSERM U565, CNRS UMR 5153, 43 rue Cuvier, 75231 Paris Cedex 05, France</json:string></affiliations></json:item></author><language><json:string>eng</json:string></language><originalGenre><json:string>other</json:string></originalGenre><abstract>The melting of tetramolecular DNA or RNA quadruplexes is kinetically irreversible. However, rather than being a hindrance, this kinetic inertia allows us to study association and dissociation processes independently. From a kinetic point of view, the association reaction is fourth order in monomer and the dissociation first order in quadruplex. The association rate constant k on, expressed in M−3·s−1 decreases with increasing temperature, reflecting a negative activation energy (E on) for the sequences presented here. Association is favored by an increase in monocation concentration. The first-order dissociation process is temperature dependent, with a very positive activation energy E off, but nearly ionic strength independent. General rules may be drawn up for various DNA and RNA sequence motifs, involving 3–6 consecutive guanines and 0–5 protruding bases. RNA quadruplexes are more stable than their DNA counterparts as a result of both faster association and slower dissociation. In most cases, no dissociation is found for G-tracts of 5 guanines or more in sodium, 4 guanines or more in potassium. The data collected here allow us to predict the amount of time required for 50% (or 90%) quadruplex formation as a function of strand sequence and concentration, temperature and ionic strength.</abstract><qualityIndicators><score>7.364</score><pdfVersion>1.3</pdfVersion><pdfPageSize>612 x 794 pts</pdfPageSize><refBibsNative>false</refBibsNative><keywordCount>0</keywordCount><abstractCharCount>1308</abstractCharCount><pdfWordCount>9389</pdfWordCount><pdfCharCount>60268</pdfCharCount><pdfPageCount>14</pdfPageCount><abstractWordCount>197</abstractWordCount></qualityIndicators><title>Kinetics of tetramolecular quadruplexes</title><genre><json:string>other</json:string></genre><host><volume>33</volume><publisherId><json:string>nar</json:string></publisherId><pages><last>94</last><first>81</first></pages><issn><json:string>0305-1048</json:string></issn><issue>1</issue><genre><json:string>journal</json:string></genre><language><json:string>unknown</json:string></language><eissn><json:string>1362-4962</json:string></eissn><title>Nucleic Acids Research</title></host><categories><wos><json:string>BIOCHEMISTRY & MOLECULAR BIOLOGY</json:string></wos></categories><publicationDate>2005</publicationDate><copyrightDate>2005</copyrightDate><doi><json:string>10.1093/nar/gki148</json:string></doi><id>ACB09B8BED86DAF85CD2EA98E69A35F8744F379C</id><score>0.24773598</score><fulltext><json:item><original>true</original><mimetype>application/pdf</mimetype><extension>pdf</extension><uri>https://api.istex.fr/document/ACB09B8BED86DAF85CD2EA98E69A35F8744F379C/fulltext/pdf</uri></json:item><json:item><original>false</original><mimetype>application/zip</mimetype><extension>zip</extension><uri>https://api.istex.fr/document/ACB09B8BED86DAF85CD2EA98E69A35F8744F379C/fulltext/zip</uri></json:item><istex:fulltextTEI uri="https://api.istex.fr/document/ACB09B8BED86DAF85CD2EA98E69A35F8744F379C/fulltext/tei"><teiHeader><fileDesc><titleStmt><title level="a" type="main" xml:lang="en">Kinetics of tetramolecular quadruplexes</title><respStmt><resp>Références bibliographiques récupérées via GROBID</resp><name resp="ISTEX-API">ISTEX-API (INIST-CNRS)</name></respStmt></titleStmt><publicationStmt><authority>ISTEX</authority><publisher>Oxford University Press</publisher><availability><p>© 2005, the authors Nucleic Acids Research, Vol. 33 No. 1 © Oxford University Press 2005; all rights reserved
The online version of this article has been published under an open access model. Users are entitled to use, reproduce, disseminate, or display the open access version of this article for non-commercial purposes provided that: the original authorship is properly and fully attributed; the Journal and Oxford University Press are attributed as the original place of publication with the correct citation details given; if an article is subsequently reproduced or disseminated not in its entirety but only in part or as a derivative work this must be clearly indicated. For commercial re-use permissions, please contact journals.permissions@oupjournals.org.</p></availability><date>2005</date></publicationStmt><notesStmt><note>*To whom correspondence should be addressed. Tel: +33 1 40 79 36 89; Fax: +33 1 40 79 37 05; Email: mergny@vnumail.com</note></notesStmt><sourceDesc><biblStruct type="inbook"><analytic><title level="a" type="main" xml:lang="en">Kinetics of tetramolecular quadruplexes</title><author xml:id="author-1"><persName><forename type="first">Jean-Louis</forename><surname>Mergny</surname></persName><affiliation>Laboratoire de Biophysique, Muséum National d'Histoire Naturelle USM503 INSERM U565, CNRS UMR 5153, 43 rue Cuvier, 75231 Paris Cedex 05, France</affiliation></author><author xml:id="author-2"><persName><forename type="first">Anne</forename><surname>De Cian</surname></persName><affiliation>Laboratoire de Biophysique, Muséum National d'Histoire Naturelle USM503 INSERM U565, CNRS UMR 5153, 43 rue Cuvier, 75231 Paris Cedex 05, France</affiliation></author><author xml:id="author-3"><persName><forename type="first">Amar</forename><surname>Ghelab</surname></persName><affiliation>Laboratoire de Biophysique, Muséum National d'Histoire Naturelle USM503 INSERM U565, CNRS UMR 5153, 43 rue Cuvier, 75231 Paris Cedex 05, France</affiliation></author><author xml:id="author-4"><persName><forename type="first">Barbara</forename><surname>Saccà</surname></persName><affiliation>Laboratoire de Biophysique, Muséum National d'Histoire Naturelle USM503 INSERM U565, CNRS UMR 5153, 43 rue Cuvier, 75231 Paris Cedex 05, France</affiliation></author><author xml:id="author-5"><persName><forename type="first">Laurent</forename><surname>Lacroix</surname></persName><affiliation>Laboratoire de Biophysique, Muséum National d'Histoire Naturelle USM503 INSERM U565, CNRS UMR 5153, 43 rue Cuvier, 75231 Paris Cedex 05, France</affiliation></author></analytic><monogr><title level="j">Nucleic Acids Research</title><title level="j" type="abbrev">Nucl. Acids Res.</title><idno type="pISSN">0305-1048</idno><idno type="eISSN">1362-4962</idno><imprint><publisher>Oxford University Press</publisher><date type="published" when="2005"></date><biblScope unit="volume">33</biblScope><biblScope unit="issue">1</biblScope><biblScope unit="page" from="81">81</biblScope><biblScope unit="page" to="94">94</biblScope></imprint></monogr><idno type="istex">ACB09B8BED86DAF85CD2EA98E69A35F8744F379C</idno><idno type="DOI">10.1093/nar/gki148</idno><idno type="local">gki148</idno></biblStruct></sourceDesc></fileDesc><profileDesc><creation><date>2005</date></creation><langUsage><language ident="en">en</language></langUsage><abstract xml:lang="en"><p>The melting of tetramolecular DNA or RNA quadruplexes is kinetically irreversible. However, rather than being a hindrance, this kinetic inertia allows us to study association and dissociation processes independently. From a kinetic point of view, the association reaction is fourth order in monomer and the dissociation first order in quadruplex. The association rate constant k on, expressed in M−3·s−1 decreases with increasing temperature, reflecting a negative activation energy (E on) for the sequences presented here. Association is favored by an increase in monocation concentration. The first-order dissociation process is temperature dependent, with a very positive activation energy E off, but nearly ionic strength independent. General rules may be drawn up for various DNA and RNA sequence motifs, involving 3–6 consecutive guanines and 0–5 protruding bases. RNA quadruplexes are more stable than their DNA counterparts as a result of both faster association and slower dissociation. In most cases, no dissociation is found for G-tracts of 5 guanines or more in sodium, 4 guanines or more in potassium. The data collected here allow us to predict the amount of time required for 50% (or 90%) quadruplex formation as a function of strand sequence and concentration, temperature and ionic strength.</p></abstract></profileDesc><revisionDesc><change when="2005">Published</change><change xml:id="refBibs-istex" who="#ISTEX-API" when="2016-10-14">References added</change></revisionDesc></teiHeader></istex:fulltextTEI><json:item><original>false</original><mimetype>text/plain</mimetype><extension>txt</extension><uri>https://api.istex.fr/document/ACB09B8BED86DAF85CD2EA98E69A35F8744F379C/fulltext/txt</uri></json:item></fulltext><metadata><istex:metadataXml wicri:clean="corpus oup" wicri:toSee="no header"><istex:xmlDeclaration>version="1.0" encoding="US-ASCII"</istex:xmlDeclaration><istex:docType PUBLIC="-//NLM//DTD Journal Publishing DTD v2.3 20070202//EN" URI="journalpublishing.dtd" name="istex:docType"></istex:docType><istex:document><article xml:lang="en" article-type="other"><front><journal-meta><journal-id journal-id-type="hwp">nar</journal-id><journal-id journal-id-type="nlm-ta">Nucleic Acids Res</journal-id><journal-id journal-id-type="publisher-id">nar</journal-id><journal-title>Nucleic Acids Research</journal-title><abbrev-journal-title abbrev-type="publisher">Nucl. Acids Res.</abbrev-journal-title><issn pub-type="ppub">0305-1048</issn><issn pub-type="epub">1362-4962</issn><publisher><publisher-name>Oxford University Press</publisher-name></publisher></journal-meta><article-meta><article-id pub-id-type="other">gki148</article-id><article-id pub-id-type="doi">10.1093/nar/gki148</article-id><article-categories><subj-group subj-group-type="heading"><subject>Article</subject></subj-group></article-categories><title-group><article-title>Kinetics of tetramolecular quadruplexes</article-title></title-group><contrib-group><contrib contrib-type="author"><name><surname>Mergny</surname><given-names>Jean-Louis</given-names></name><xref rid="COR1">*</xref></contrib><contrib contrib-type="author"><name><surname>De Cian</surname><given-names>Anne</given-names></name></contrib><contrib contrib-type="author"><name><surname>Ghelab</surname><given-names>Amar</given-names></name></contrib><contrib contrib-type="author"><name><surname>Saccà</surname><given-names>Barbara</given-names></name></contrib><contrib contrib-type="author"><name><surname>Lacroix</surname><given-names>Laurent</given-names></name></contrib><aff> Laboratoire de Biophysique, Muséum National d'Histoire Naturelle USM503 INSERM U565, CNRS UMR 5153, 43 rue Cuvier, 75231 Paris Cedex 05, France </aff></contrib-group><author-notes><corresp id="COR1"><sup>*</sup>To whom correspondence should be addressed. Tel: +33 1 40 79 36 89; Fax: +33 1 40 79 37 05; Email: <ext-link xlink:href="mergny@vnumail.com" ext-link-type="email">mergny@vnumail.com</ext-link></corresp></author-notes><pub-date pub-type="ppub"><year>2005</year></pub-date><volume>33</volume><issue>1</issue><fpage>81</fpage><lpage>94</lpage><history><date date-type="accepted"><day>07</day><month>12</month><year>2004</year></date><date date-type="received"><day>21</day><month>10</month><year>2004</year></date><date date-type="rev-recd"><day>07</day><month>12</month><year>2004</year></date></history><permissions><copyright-statement>© 2005, the authors <italic>Nucleic Acids Research, Vol. 33 No. 1</italic> © <italic>Oxford University Press 2005; all rights reserved</italic>
The online version of this article has been published under an open access model. Users are entitled to use, reproduce, disseminate, or display the open access version of this article for non-commercial purposes provided that: the original authorship is properly and fully attributed; the Journal and Oxford University Press are attributed as the original place of publication with the correct citation details given; if an article is subsequently reproduced or disseminated not in its entirety but only in part or as a derivative work this must be clearly indicated. For commercial re-use permissions, please contact <ext-link xlink:href="journals.permissions@oupjournals.org" ext-link-type="email">journals.permissions@oupjournals.org</ext-link>.</copyright-statement><copyright-year>2005</copyright-year><license license-type="open-access"><p></p></license></permissions><abstract xml:lang="en"><p>The melting of tetramolecular DNA or RNA quadruplexes is kinetically irreversible. However, rather than being a hindrance, this kinetic inertia allows us to study association and dissociation processes independently. From a kinetic point of view, the association reaction is fourth order in monomer and the dissociation first order in quadruplex. The association rate constant <italic>k</italic> <sub>on</sub>, expressed in M<sup>−3</sup>·s<sup>−1</sup> decreases with increasing temperature, reflecting a negative activation energy (<italic>E</italic> <sub>on</sub>) for the sequences presented here. Association is favored by an increase in monocation concentration. The first-order dissociation process is temperature dependent, with a very positive activation energy <italic>E</italic> <sub>off</sub>, but nearly ionic strength independent. General rules may be drawn up for various DNA and RNA sequence motifs, involving 3–6 consecutive guanines and 0–5 protruding bases. RNA quadruplexes are more stable than their DNA counterparts as a result of both faster association and slower dissociation. In most cases, no dissociation is found for G-tracts of 5 guanines or more in sodium, 4 guanines or more in potassium. The data collected here allow us to predict the amount of time required for 50% (or 90%) quadruplex formation as a function of strand sequence and concentration, temperature and ionic strength.</p></abstract><custom-meta-wrap><custom-meta><meta-name>hwp-legacy-fpage</meta-name><meta-value>81</meta-value></custom-meta><custom-meta><meta-name>hwp-legacy-dochead</meta-name><meta-value>research-article</meta-value></custom-meta></custom-meta-wrap></article-meta></front></article></istex:document></istex:metadataXml><mods version="3.6"><titleInfo lang="en"><title>Kinetics of tetramolecular quadruplexes</title></titleInfo><titleInfo type="alternative" lang="en" contentType="CDATA"><title>Kinetics of tetramolecular quadruplexes</title></titleInfo><name type="personal"><namePart type="given">Jean-Louis</namePart><namePart type="family">Mergny</namePart><affiliation>Laboratoire de Biophysique, Muséum National d'Histoire Naturelle USM503 INSERM U565, CNRS UMR 5153, 43 rue Cuvier, 75231 Paris Cedex 05, France</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">Anne</namePart><namePart type="family">De Cian</namePart><affiliation>Laboratoire de Biophysique, Muséum National d'Histoire Naturelle USM503 INSERM U565, CNRS UMR 5153, 43 rue Cuvier, 75231 Paris Cedex 05, France</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">Amar</namePart><namePart type="family">Ghelab</namePart><affiliation>Laboratoire de Biophysique, Muséum National d'Histoire Naturelle USM503 INSERM U565, CNRS UMR 5153, 43 rue Cuvier, 75231 Paris Cedex 05, France</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">Barbara</namePart><namePart type="family">Saccà</namePart><affiliation>Laboratoire de Biophysique, Muséum National d'Histoire Naturelle USM503 INSERM U565, CNRS UMR 5153, 43 rue Cuvier, 75231 Paris Cedex 05, France</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">Laurent</namePart><namePart type="family">Lacroix</namePart><affiliation>Laboratoire de Biophysique, Muséum National d'Histoire Naturelle USM503 INSERM U565, CNRS UMR 5153, 43 rue Cuvier, 75231 Paris Cedex 05, France</affiliation><role><roleTerm type="text">author</roleTerm></role></name><typeOfResource>text</typeOfResource><genre type="other" displayLabel="other"></genre><originInfo><publisher>Oxford University Press</publisher><dateIssued encoding="w3cdtf">2005</dateIssued><copyrightDate encoding="w3cdtf">2005</copyrightDate></originInfo><language><languageTerm type="code" authority="iso639-2b">eng</languageTerm><languageTerm type="code" authority="rfc3066">en</languageTerm></language><physicalDescription><internetMediaType>text/html</internetMediaType></physicalDescription><abstract lang="en">The melting of tetramolecular DNA or RNA quadruplexes is kinetically irreversible. However, rather than being a hindrance, this kinetic inertia allows us to study association and dissociation processes independently. From a kinetic point of view, the association reaction is fourth order in monomer and the dissociation first order in quadruplex. The association rate constant k on, expressed in M−3·s−1 decreases with increasing temperature, reflecting a negative activation energy (E on) for the sequences presented here. Association is favored by an increase in monocation concentration. The first-order dissociation process is temperature dependent, with a very positive activation energy E off, but nearly ionic strength independent. General rules may be drawn up for various DNA and RNA sequence motifs, involving 3–6 consecutive guanines and 0–5 protruding bases. RNA quadruplexes are more stable than their DNA counterparts as a result of both faster association and slower dissociation. In most cases, no dissociation is found for G-tracts of 5 guanines or more in sodium, 4 guanines or more in potassium. The data collected here allow us to predict the amount of time required for 50% (or 90%) quadruplex formation as a function of strand sequence and concentration, temperature and ionic strength.</abstract><note type="author-notes">*To whom correspondence should be addressed. Tel: +33 1 40 79 36 89; Fax: +33 1 40 79 37 05; Email: mergny@vnumail.com</note><relatedItem type="host"><titleInfo><title>Nucleic Acids Research</title></titleInfo><titleInfo type="abbreviated"><title>Nucl. Acids Res.</title></titleInfo><genre type="journal">journal</genre><identifier type="ISSN">0305-1048</identifier><identifier type="eISSN">1362-4962</identifier><identifier type="PublisherID">nar</identifier><identifier type="PublisherID-hwp">nar</identifier><identifier type="PublisherID-nlm-ta">Nucleic Acids Res</identifier><part><date>2005</date><detail type="volume"><caption>vol.</caption><number>33</number></detail><detail type="issue"><caption>no.</caption><number>1</number></detail><extent unit="pages"><start>81</start><end>94</end></extent></part></relatedItem><identifier type="istex">ACB09B8BED86DAF85CD2EA98E69A35F8744F379C</identifier><identifier type="DOI">10.1093/nar/gki148</identifier><identifier type="local">gki148</identifier><accessCondition type="use and reproduction" contentType="copyright">© 2005, the authors Nucleic Acids Research, Vol. 33 No. 1 © Oxford University Press 2005; all rights reserved
The online version of this article has been published under an open access model. Users are entitled to use, reproduce, disseminate, or display the open access version of this article for non-commercial purposes provided that: the original authorship is properly and fully attributed; the Journal and Oxford University Press are attributed as the original place of publication with the correct citation details given; if an article is subsequently reproduced or disseminated not in its entirety but only in part or as a derivative work this must be clearly indicated. For commercial re-use permissions, please contact journals.permissions@oupjournals.org.</accessCondition><recordInfo><recordContentSource>OUP</recordContentSource></recordInfo></mods></metadata><annexes><json:item><original>true</original><mimetype>image/jpeg</mimetype><extension>jpeg</extension><uri>https://api.istex.fr/document/ACB09B8BED86DAF85CD2EA98E69A35F8744F379C/annexes/jpeg</uri></json:item><json:item><original>true</original><mimetype>image/gif</mimetype><extension>gif</extension><uri>https://api.istex.fr/document/ACB09B8BED86DAF85CD2EA98E69A35F8744F379C/annexes/gif</uri></json:item></annexes><enrichments><istex:catWosTEI uri="https://api.istex.fr/document/ACB09B8BED86DAF85CD2EA98E69A35F8744F379C/enrichments/catWos"><teiHeader><profileDesc><textClass><classCode scheme="WOS">BIOCHEMISTRY & MOLECULAR BIOLOGY</classCode></textClass></profileDesc></teiHeader></istex:catWosTEI><json:item><type>refBibs</type><uri>https://api.istex.fr/document/ACB09B8BED86DAF85CD2EA98E69A35F8744F379C/enrichments/refBibs</uri></json:item></enrichments><serie></serie></istex></record>Pour manipuler ce document sous Unix (Dilib)
EXPLOR_STEP=$WICRI_ROOT/Wicri/Belgique/explor/OpenAccessBelV2/Data/Istex/Corpus
HfdSelect -h $EXPLOR_STEP/biblio.hfd -nk 000F03 | SxmlIndent | more
Ou
HfdSelect -h $EXPLOR_AREA/Data/Istex/Corpus/biblio.hfd -nk 000F03 | SxmlIndent | more
Pour mettre un lien sur cette page dans le réseau Wicri
{{Explor lien
|wiki= Wicri/Belgique
|area= OpenAccessBelV2
|flux= Istex
|étape= Corpus
|type= RBID
|clé= ISTEX:ACB09B8BED86DAF85CD2EA98E69A35F8744F379C
|texte= Kinetics of tetramolecular quadruplexes
}}
| This area was generated with Dilib version V0.6.25. |  |