An in vivo culture system for human embryos using an encapsulation technology: a pilot study
Identifieur interne : 000A41 ( Istex/Corpus ); précédent : 000A40; suivant : 000A42An in vivo culture system for human embryos using an encapsulation technology: a pilot study
Auteurs : C. Blockeel ; P. Mock ; G. Verheyen ; N. Bouche ; Ph. Le Goff ; Y. Heyman ; C. Wrenzycki ; K. Hffmann ; H. Niemann ; P. Haentjens ; M. J. De Los Santos ; M. Fernandez-Sanchez ; M. Velasco ; P. Aebischer ; P. Devroey ; C. SimnSource :
- Human Reproduction [ 0268-1161 ] ; 2009-04.
Abstract
BACKGROUND Animal studies have demonstrated better embryo development in vivo than in vitro. This pilot study tested the feasibility of using a novel in utero culture system (IUCS) to obtain normal human fertilization and embryo development. METHODS The IUCS device comprised a perforated silicone hollow tube. The study included 13 patients (<36 years) undergoing a first intracytoplasmic sperm injection (ICSI) treatment and 167 metaphase II oocytes in three groups. In Group 1, 12 h after ICSI, sibling oocytes were assigned to IUCS or conventional in vitro culture. The device was retrieved on Day 1, and all zygotes were cultured in vitro till Day 5. In Group 2, fertilized oocytes were assigned on Day 1, embryos retrieved on Day 3 and all embryos cultured till Day 5. In Group 3, after Day 0 assignment, embryos were retrieved on Day 3 for blastomere biopsy and fluorescence in situ hybridization (FISH) and cultured until Day 5. The highest quality blastocysts were transferred on Day 5. RESULTS Fertilization and embryo development were comparable in the in vitro and IUCS arms, with a tendency towards better embryo quality in the IUCS. FISH analysis in Group 3 revealed more normal embryos using the IUCS (P 0.049). Three clinical pregnancies and live births were obtained: two from the IUCS arm and one from the in vitro arm. CONCLUSIONS Our pilot study shows that this new IUCS appears to be feasible and safe, supporting normal fertilization, embryo development and normal chromosomal segregation. Furthermore, live births are possible after the transient presence of a silicone device in the uterus.Clinicaltrials.gov: NCT00480103.
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DOI: 10.1093/humrep/dep005
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Verheyen</name><affiliation><mods:affiliation>Centre for Reproductive Medicine, UZ Brussel, Brussels, Belgium</mods:affiliation></affiliation></author><author><name sortKey="Bouche, N" sort="Bouche, N" uniqKey="Bouche N" first="N." last="Bouche">N. Bouche</name><affiliation><mods:affiliation>Anecova Inc., Lausanne, Switzerland</mods:affiliation></affiliation><affiliation><mods:affiliation>Brain and Mind Institute, Ecole Polytechnique Fdrale de Lausanne, EPFL, Lausanne, Switzerland</mods:affiliation></affiliation></author><author><name sortKey="Le Goff, Ph" sort="Le Goff, Ph" uniqKey="Le Goff P" first="Ph." last="Le Goff">Ph. Le Goff</name><affiliation><mods:affiliation>Anecova Inc., Lausanne, Switzerland</mods:affiliation></affiliation></author><author><name sortKey="Heyman, Y" sort="Heyman, Y" uniqKey="Heyman Y" first="Y." last="Heyman">Y. 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Niemann</name><affiliation><mods:affiliation>Institut fr Nutztiergenetik, FLI, Mariensee, Neustadt, Germany</mods:affiliation></affiliation></author><author><name sortKey="Haentjens, P" sort="Haentjens, P" uniqKey="Haentjens P" first="P." last="Haentjens">P. Haentjens</name><affiliation><mods:affiliation>Centre for Outcomes Research and Laboratory for Experimental Surgery, UZ Brussel, Brussels, Belgium</mods:affiliation></affiliation></author><author><name sortKey="De Los Santos, M J" sort="De Los Santos, M J" uniqKey="De Los Santos M" first="M. J." last="De Los Santos">M. J. De Los Santos</name><affiliation><mods:affiliation>Instituto Valenciano de Infertilidad (IVI), Valencia, Spain</mods:affiliation></affiliation></author><author><name sortKey="Fernandez Sanchez, M" sort="Fernandez Sanchez, M" uniqKey="Fernandez Sanchez M" first="M." last="Fernandez-Sanchez">M. 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Simn</name><affiliation><mods:affiliation>Instituto Valenciano de Infertilidad (IVI), Valencia, Spain</mods:affiliation></affiliation><affiliation><mods:affiliation>E-mail: csimon@ivi.es</mods:affiliation></affiliation></author></titleStmt><publicationStmt><idno type="wicri:source">ISTEX</idno><idno type="RBID">ISTEX:033DA12265BEEFC4242E6536902363F8C6BE7EA5</idno><date when="2009" year="2009">2009</date><idno type="doi">10.1093/humrep/dep005</idno><idno type="url">https://api.istex.fr/document/033DA12265BEEFC4242E6536902363F8C6BE7EA5/fulltext/pdf</idno><idno type="wicri:Area/Istex/Corpus">000A41</idno></publicationStmt><sourceDesc><biblStruct><analytic><title level="a">An in vivo culture system for human embryos using an encapsulation technology: a pilot study</title><author><name sortKey="Blockeel, C" sort="Blockeel, C" uniqKey="Blockeel C" first="C." last="Blockeel">C. Blockeel</name><affiliation><mods:affiliation>Centre for Reproductive Medicine, UZ Brussel, Brussels, Belgium</mods:affiliation></affiliation></author><author><name sortKey="Mock, P" sort="Mock, P" uniqKey="Mock P" first="P." last="Mock">P. Mock</name><affiliation><mods:affiliation>Anecova Inc., Lausanne, Switzerland</mods:affiliation></affiliation><affiliation><mods:affiliation>University of Geneva, Geneva, Switzerland</mods:affiliation></affiliation></author><author><name sortKey="Verheyen, G" sort="Verheyen, G" uniqKey="Verheyen G" first="G." last="Verheyen">G. Verheyen</name><affiliation><mods:affiliation>Centre for Reproductive Medicine, UZ Brussel, Brussels, Belgium</mods:affiliation></affiliation></author><author><name sortKey="Bouche, N" sort="Bouche, N" uniqKey="Bouche N" first="N." last="Bouche">N. Bouche</name><affiliation><mods:affiliation>Anecova Inc., Lausanne, Switzerland</mods:affiliation></affiliation><affiliation><mods:affiliation>Brain and Mind Institute, Ecole Polytechnique Fdrale de Lausanne, EPFL, Lausanne, Switzerland</mods:affiliation></affiliation></author><author><name sortKey="Le Goff, Ph" sort="Le Goff, Ph" uniqKey="Le Goff P" first="Ph." last="Le Goff">Ph. Le Goff</name><affiliation><mods:affiliation>Anecova Inc., Lausanne, Switzerland</mods:affiliation></affiliation></author><author><name sortKey="Heyman, Y" sort="Heyman, Y" uniqKey="Heyman Y" first="Y." last="Heyman">Y. Heyman</name><affiliation><mods:affiliation>Institut National de Recherche Agronomique, INRA, Jouy en Josas, France</mods:affiliation></affiliation></author><author><name sortKey="Wrenzycki, C" sort="Wrenzycki, C" uniqKey="Wrenzycki C" first="C." last="Wrenzycki">C. Wrenzycki</name><affiliation><mods:affiliation>Stiftung Tierrztliche Hochschule, Klinik fr Rinder, Reproduktionsmedizinische Einheit der Kliniken, Hannover, Germany</mods:affiliation></affiliation></author><author><name sortKey="Hffmann, K" sort="Hffmann, K" uniqKey="Hffmann K" first="K." last="Hffmann">K. Hffmann</name><affiliation><mods:affiliation>Stiftung Tierrztliche Hochschule, Klinik fr Rinder, Reproduktionsmedizinische Einheit der Kliniken, Hannover, Germany</mods:affiliation></affiliation></author><author><name sortKey="Niemann, H" sort="Niemann, H" uniqKey="Niemann H" first="H." last="Niemann">H. Niemann</name><affiliation><mods:affiliation>Institut fr Nutztiergenetik, FLI, Mariensee, Neustadt, Germany</mods:affiliation></affiliation></author><author><name sortKey="Haentjens, P" sort="Haentjens, P" uniqKey="Haentjens P" first="P." last="Haentjens">P. Haentjens</name><affiliation><mods:affiliation>Centre for Outcomes Research and Laboratory for Experimental Surgery, UZ Brussel, Brussels, Belgium</mods:affiliation></affiliation></author><author><name sortKey="De Los Santos, M J" sort="De Los Santos, M J" uniqKey="De Los Santos M" first="M. J." last="De Los Santos">M. J. De Los Santos</name><affiliation><mods:affiliation>Instituto Valenciano de Infertilidad (IVI), Valencia, Spain</mods:affiliation></affiliation></author><author><name sortKey="Fernandez Sanchez, M" sort="Fernandez Sanchez, M" uniqKey="Fernandez Sanchez M" first="M." last="Fernandez-Sanchez">M. Fernandez-Sanchez</name><affiliation><mods:affiliation>Instituto Valenciano de Infertilidad (IVI), Valencia, Spain</mods:affiliation></affiliation></author><author><name sortKey="Velasco, M" sort="Velasco, M" uniqKey="Velasco M" first="M." last="Velasco">M. Velasco</name><affiliation><mods:affiliation>Anecova Inc., Lausanne, Switzerland</mods:affiliation></affiliation></author><author><name sortKey="Aebischer, P" sort="Aebischer, P" uniqKey="Aebischer P" first="P." last="Aebischer">P. Aebischer</name><affiliation><mods:affiliation>Brain and Mind Institute, Ecole Polytechnique Fdrale de Lausanne, EPFL, Lausanne, Switzerland</mods:affiliation></affiliation></author><author><name sortKey="Devroey, P" sort="Devroey, P" uniqKey="Devroey P" first="P." last="Devroey">P. Devroey</name><affiliation><mods:affiliation>Centre for Reproductive Medicine, UZ Brussel, Brussels, Belgium</mods:affiliation></affiliation></author><author><name sortKey="Simn, C" sort="Simn, C" uniqKey="Simn C" first="C." last="Simn">C. Simn</name><affiliation><mods:affiliation>Instituto Valenciano de Infertilidad (IVI), Valencia, Spain</mods:affiliation></affiliation><affiliation><mods:affiliation>E-mail: csimon@ivi.es</mods:affiliation></affiliation></author></analytic><monogr></monogr><series><title level="j">Human Reproduction</title><idno type="ISSN">0268-1161</idno><idno type="eISSN">1460-2350</idno><imprint><publisher>Oxford University Press</publisher><date type="published" when="2009-04">2009-04</date><biblScope unit="volume">24</biblScope><biblScope unit="issue">4</biblScope><biblScope unit="page" from="790">790</biblScope><biblScope unit="page" to="796">796</biblScope></imprint><idno type="ISSN">0268-1161</idno></series><idno type="istex">033DA12265BEEFC4242E6536902363F8C6BE7EA5</idno><idno type="DOI">10.1093/humrep/dep005</idno><idno type="ArticleID">dep005</idno></biblStruct></sourceDesc><seriesStmt><idno type="ISSN">0268-1161</idno></seriesStmt></fileDesc><profileDesc><textClass></textClass><langUsage><language ident="en">en</language></langUsage></profileDesc></teiHeader><front><div type="abstract">BACKGROUND Animal studies have demonstrated better embryo development in vivo than in vitro. This pilot study tested the feasibility of using a novel in utero culture system (IUCS) to obtain normal human fertilization and embryo development. METHODS The IUCS device comprised a perforated silicone hollow tube. The study included 13 patients (<36 years) undergoing a first intracytoplasmic sperm injection (ICSI) treatment and 167 metaphase II oocytes in three groups. In Group 1, 12 h after ICSI, sibling oocytes were assigned to IUCS or conventional in vitro culture. The device was retrieved on Day 1, and all zygotes were cultured in vitro till Day 5. In Group 2, fertilized oocytes were assigned on Day 1, embryos retrieved on Day 3 and all embryos cultured till Day 5. In Group 3, after Day 0 assignment, embryos were retrieved on Day 3 for blastomere biopsy and fluorescence in situ hybridization (FISH) and cultured until Day 5. The highest quality blastocysts were transferred on Day 5. RESULTS Fertilization and embryo development were comparable in the in vitro and IUCS arms, with a tendency towards better embryo quality in the IUCS. FISH analysis in Group 3 revealed more normal embryos using the IUCS (P 0.049). Three clinical pregnancies and live births were obtained: two from the IUCS arm and one from the in vitro arm. CONCLUSIONS Our pilot study shows that this new IUCS appears to be feasible and safe, supporting normal fertilization, embryo development and normal chromosomal segregation. Furthermore, live births are possible after the transient presence of a silicone device in the uterus.Clinicaltrials.gov: NCT00480103.</div></front></TEI><istex><corpusName>oup</corpusName><author><json:item><name>C. Blockeel</name><affiliations><json:string>Centre for Reproductive Medicine, UZ Brussel, Brussels, Belgium</json:string></affiliations></json:item><json:item><name>P. Mock</name><affiliations><json:string>Anecova Inc., Lausanne, Switzerland</json:string><json:string>University of Geneva, Geneva, Switzerland</json:string></affiliations></json:item><json:item><name>G. Verheyen</name><affiliations><json:string>Centre for Reproductive Medicine, UZ Brussel, Brussels, Belgium</json:string></affiliations></json:item><json:item><name>N. Bouche</name><affiliations><json:string>Anecova Inc., Lausanne, Switzerland</json:string><json:string>Brain and Mind Institute, Ecole Polytechnique Fdrale de Lausanne, EPFL, Lausanne, Switzerland</json:string></affiliations></json:item><json:item><name>Ph. Le Goff</name><affiliations><json:string>Anecova Inc., Lausanne, Switzerland</json:string></affiliations></json:item><json:item><name>Y. Heyman</name><affiliations><json:string>Institut National de Recherche Agronomique, INRA, Jouy en Josas, France</json:string></affiliations></json:item><json:item><name>C. Wrenzycki</name><affiliations><json:string>Stiftung Tierrztliche Hochschule, Klinik fr Rinder, Reproduktionsmedizinische Einheit der Kliniken, Hannover, Germany</json:string></affiliations></json:item><json:item><name>K. Hffmann</name><affiliations><json:string>Stiftung Tierrztliche Hochschule, Klinik fr Rinder, Reproduktionsmedizinische Einheit der Kliniken, Hannover, Germany</json:string></affiliations></json:item><json:item><name>H. Niemann</name><affiliations><json:string>Institut fr Nutztiergenetik, FLI, Mariensee, Neustadt, Germany</json:string></affiliations></json:item><json:item><name>P. Haentjens</name><affiliations><json:string>Centre for Outcomes Research and Laboratory for Experimental Surgery, UZ Brussel, Brussels, Belgium</json:string></affiliations></json:item><json:item><name>M.J. de Los Santos</name><affiliations><json:string>Instituto Valenciano de Infertilidad (IVI), Valencia, Spain</json:string></affiliations></json:item><json:item><name>M. Fernandez-Sanchez</name><affiliations><json:string>Instituto Valenciano de Infertilidad (IVI), Valencia, Spain</json:string></affiliations></json:item><json:item><name>M. Velasco</name><affiliations><json:string>Anecova Inc., Lausanne, Switzerland</json:string></affiliations></json:item><json:item><name>P. Aebischer</name><affiliations><json:string>Brain and Mind Institute, Ecole Polytechnique Fdrale de Lausanne, EPFL, Lausanne, Switzerland</json:string></affiliations></json:item><json:item><name>P. Devroey</name><affiliations><json:string>Centre for Reproductive Medicine, UZ Brussel, Brussels, Belgium</json:string></affiliations></json:item><json:item><name>C. Simn</name><affiliations><json:string>Instituto Valenciano de Infertilidad (IVI), Valencia, Spain</json:string><json:string>E-mail: csimon@ivi.es</json:string></affiliations></json:item></author><subject><json:item><lang><json:string>eng</json:string></lang><value>in vivo culture</value></json:item><json:item><lang><json:string>eng</json:string></lang><value>in utero culture system</value></json:item><json:item><lang><json:string>eng</json:string></lang><value>ICSI</value></json:item><json:item><lang><json:string>eng</json:string></lang><value>embryos</value></json:item><json:item><lang><json:string>eng</json:string></lang><value>oocytes</value></json:item></subject><articleId><json:string>dep005</json:string></articleId><language><json:string>eng</json:string></language><originalGenre><json:string>research-article</json:string></originalGenre><abstract>BACKGROUND Animal studies have demonstrated better embryo development in vivo than in vitro. This pilot study tested the feasibility of using a novel in utero culture system (IUCS) to obtain normal human fertilization and embryo development. METHODS The IUCS device comprised a perforated silicone hollow tube. The study included 13 patients (>36 years) undergoing a first intracytoplasmic sperm injection (ICSI) treatment and 167 metaphase II oocytes in three groups. In Group 1, 12 h after ICSI, sibling oocytes were assigned to IUCS or conventional in vitro culture. The device was retrieved on Day 1, and all zygotes were cultured in vitro till Day 5. In Group 2, fertilized oocytes were assigned on Day 1, embryos retrieved on Day 3 and all embryos cultured till Day 5. In Group 3, after Day 0 assignment, embryos were retrieved on Day 3 for blastomere biopsy and fluorescence in situ hybridization (FISH) and cultured until Day 5. The highest quality blastocysts were transferred on Day 5. RESULTS Fertilization and embryo development were comparable in the in vitro and IUCS arms, with a tendency towards better embryo quality in the IUCS. FISH analysis in Group 3 revealed more normal embryos using the IUCS (P 0.049). Three clinical pregnancies and live births were obtained: two from the IUCS arm and one from the in vitro arm. CONCLUSIONS Our pilot study shows that this new IUCS appears to be feasible and safe, supporting normal fertilization, embryo development and normal chromosomal segregation. Furthermore, live births are possible after the transient presence of a silicone device in the uterus.Clinicaltrials.gov: NCT00480103.</abstract><qualityIndicators><score>8.826</score><pdfVersion>1.5</pdfVersion><pdfPageSize>612.283 x 790.866 pts</pdfPageSize><refBibsNative>true</refBibsNative><keywordCount>5</keywordCount><abstractCharCount>1646</abstractCharCount><pdfWordCount>4826</pdfWordCount><pdfCharCount>29486</pdfCharCount><pdfPageCount>7</pdfPageCount><abstractWordCount>261</abstractWordCount></qualityIndicators><title>An in vivo culture system for human embryos using an encapsulation technology: a pilot study</title><genre><json:string>research-article</json:string></genre><host><volume>24</volume><publisherId><json:string>humrep</json:string></publisherId><pages><last>796</last><first>790</first></pages><issn><json:string>0268-1161</json:string></issn><issue>4</issue><genre><json:string>journal</json:string></genre><language><json:string>unknown</json:string></language><eissn><json:string>1460-2350</json:string></eissn><title>Human Reproduction</title></host><categories><wos><json:string>OBSTETRICS & GYNECOLOGY</json:string><json:string>REPRODUCTIVE BIOLOGY</json:string></wos></categories><publicationDate>2009</publicationDate><copyrightDate>2009</copyrightDate><doi><json:string>10.1093/humrep/dep005</json:string></doi><id>033DA12265BEEFC4242E6536902363F8C6BE7EA5</id><score>0.2650633</score><fulltext><json:item><original>true</original><mimetype>application/pdf</mimetype><extension>pdf</extension><uri>https://api.istex.fr/document/033DA12265BEEFC4242E6536902363F8C6BE7EA5/fulltext/pdf</uri></json:item><json:item><original>false</original><mimetype>application/zip</mimetype><extension>zip</extension><uri>https://api.istex.fr/document/033DA12265BEEFC4242E6536902363F8C6BE7EA5/fulltext/zip</uri></json:item><istex:fulltextTEI uri="https://api.istex.fr/document/033DA12265BEEFC4242E6536902363F8C6BE7EA5/fulltext/tei"><teiHeader><fileDesc><titleStmt><title level="a">An in vivo culture system for human embryos using an encapsulation technology: a pilot study</title></titleStmt><publicationStmt><authority>ISTEX</authority><publisher>Oxford University Press</publisher><availability><p>The Author 2009. Published by Oxford University Press on behalf of the European Society of Human Reproduction and Embryology. All rights reserved. For Permissions, please email: journals.permissionsoxfordjournals.org</p></availability><date>2009-03-10</date></publicationStmt><notesStmt><note>The first three authors have contributed equally.</note></notesStmt><sourceDesc><biblStruct type="inbook"><analytic><title level="a">An in vivo culture system for human embryos using an encapsulation technology: a pilot study</title><author xml:id="author-1"><persName><forename type="first">C.</forename><surname>Blockeel</surname></persName><note type="biography">The first three authors have contributed equally.</note><affiliation>The first three authors have contributed equally.</affiliation><affiliation>Centre for Reproductive Medicine, UZ Brussel, Brussels, Belgium</affiliation></author><author xml:id="author-2"><persName><forename type="first">P.</forename><surname>Mock</surname></persName><note type="biography">The first three authors have contributed equally.</note><affiliation>The first three authors have contributed equally.</affiliation><affiliation>Anecova Inc., Lausanne, Switzerland</affiliation><affiliation>University of Geneva, Geneva, Switzerland</affiliation></author><author xml:id="author-3"><persName><forename type="first">G.</forename><surname>Verheyen</surname></persName><note type="biography">The first three authors have contributed equally.</note><affiliation>The first three authors have contributed equally.</affiliation><affiliation>Centre for Reproductive Medicine, UZ Brussel, Brussels, Belgium</affiliation></author><author xml:id="author-4"><persName><forename type="first">N.</forename><surname>Bouche</surname></persName><affiliation>Anecova Inc., Lausanne, Switzerland</affiliation><affiliation>Brain and Mind Institute, Ecole Polytechnique Fdrale de Lausanne, EPFL, Lausanne, Switzerland</affiliation></author><author xml:id="author-5"><persName><forename type="first">Ph.</forename><surname>Le Goff</surname></persName><affiliation>Anecova Inc., Lausanne, Switzerland</affiliation></author><author xml:id="author-6"><persName><forename type="first">Y.</forename><surname>Heyman</surname></persName><affiliation>Institut National de Recherche Agronomique, INRA, Jouy en Josas, France</affiliation></author><author xml:id="author-7"><persName><forename type="first">C.</forename><surname>Wrenzycki</surname></persName><affiliation>Stiftung Tierrztliche Hochschule, Klinik fr Rinder, Reproduktionsmedizinische Einheit der Kliniken, Hannover, Germany</affiliation></author><author xml:id="author-8"><persName><forename type="first">K.</forename><surname>Hffmann</surname></persName><affiliation>Stiftung Tierrztliche Hochschule, Klinik fr Rinder, Reproduktionsmedizinische Einheit der Kliniken, Hannover, Germany</affiliation></author><author xml:id="author-9"><persName><forename type="first">H.</forename><surname>Niemann</surname></persName><affiliation>Institut fr Nutztiergenetik, FLI, Mariensee, Neustadt, Germany</affiliation></author><author xml:id="author-10"><persName><forename type="first">P.</forename><surname>Haentjens</surname></persName><affiliation>Centre for Outcomes Research and Laboratory for Experimental Surgery, UZ Brussel, Brussels, Belgium</affiliation></author><author xml:id="author-11"><persName><forename type="first">M.J.</forename><surname>de Los Santos</surname></persName><affiliation>Instituto Valenciano de Infertilidad (IVI), Valencia, Spain</affiliation></author><author xml:id="author-12"><persName><forename type="first">M.</forename><surname>Fernandez-Sanchez</surname></persName><affiliation>Instituto Valenciano de Infertilidad (IVI), Valencia, Spain</affiliation></author><author xml:id="author-13"><persName><forename type="first">M.</forename><surname>Velasco</surname></persName><affiliation>Anecova Inc., Lausanne, Switzerland</affiliation></author><author xml:id="author-14"><persName><forename type="first">P.</forename><surname>Aebischer</surname></persName><affiliation>Brain and Mind Institute, Ecole Polytechnique Fdrale de Lausanne, EPFL, Lausanne, Switzerland</affiliation></author><author xml:id="author-15"><persName><forename type="first">P.</forename><surname>Devroey</surname></persName><affiliation>Centre for Reproductive Medicine, UZ Brussel, Brussels, Belgium</affiliation></author><author xml:id="author-16"><persName><forename type="first">C.</forename><surname>Simn</surname></persName><email>csimon@ivi.es</email><affiliation>Instituto Valenciano de Infertilidad (IVI), Valencia, Spain</affiliation></author></analytic><monogr><title level="j">Human Reproduction</title><idno type="pISSN">0268-1161</idno><idno type="eISSN">1460-2350</idno><imprint><publisher>Oxford University Press</publisher><date type="published" when="2009-04"></date><biblScope unit="volume">24</biblScope><biblScope unit="issue">4</biblScope><biblScope unit="page" from="790">790</biblScope><biblScope unit="page" to="796">796</biblScope></imprint></monogr><idno type="istex">033DA12265BEEFC4242E6536902363F8C6BE7EA5</idno><idno type="DOI">10.1093/humrep/dep005</idno><idno type="ArticleID">dep005</idno></biblStruct></sourceDesc></fileDesc><profileDesc><creation><date>2009-03-10</date></creation><langUsage><language ident="en">en</language></langUsage><abstract><p>BACKGROUND Animal studies have demonstrated better embryo development in vivo than in vitro. This pilot study tested the feasibility of using a novel in utero culture system (IUCS) to obtain normal human fertilization and embryo development. METHODS The IUCS device comprised a perforated silicone hollow tube. The study included 13 patients (<36 years) undergoing a first intracytoplasmic sperm injection (ICSI) treatment and 167 metaphase II oocytes in three groups. In Group 1, 12 h after ICSI, sibling oocytes were assigned to IUCS or conventional in vitro culture. The device was retrieved on Day 1, and all zygotes were cultured in vitro till Day 5. In Group 2, fertilized oocytes were assigned on Day 1, embryos retrieved on Day 3 and all embryos cultured till Day 5. In Group 3, after Day 0 assignment, embryos were retrieved on Day 3 for blastomere biopsy and fluorescence in situ hybridization (FISH) and cultured until Day 5. The highest quality blastocysts were transferred on Day 5. RESULTS Fertilization and embryo development were comparable in the in vitro and IUCS arms, with a tendency towards better embryo quality in the IUCS. FISH analysis in Group 3 revealed more normal embryos using the IUCS (P 0.049). Three clinical pregnancies and live births were obtained: two from the IUCS arm and one from the in vitro arm. CONCLUSIONS Our pilot study shows that this new IUCS appears to be feasible and safe, supporting normal fertilization, embryo development and normal chromosomal segregation. Furthermore, live births are possible after the transient presence of a silicone device in the uterus.Clinicaltrials.gov: NCT00480103.</p></abstract><textClass><keywords scheme="keyword"><list><head>Keywords</head><item><term>in vivo culture</term></item><item><term>in utero culture system</term></item><item><term>ICSI</term></item><item><term>embryos</term></item><item><term>oocytes</term></item></list></keywords></textClass></profileDesc><revisionDesc><change when="2009-03-10">Created</change><change when="2009-04">Published</change></revisionDesc></teiHeader></istex:fulltextTEI><json:item><original>false</original><mimetype>text/plain</mimetype><extension>txt</extension><uri>https://api.istex.fr/document/033DA12265BEEFC4242E6536902363F8C6BE7EA5/fulltext/txt</uri></json:item></fulltext><metadata><istex:metadataXml wicri:clean="corpus oup" wicri:toSee="no header"><istex:xmlDeclaration>version="1.0" encoding="utf-8"</istex:xmlDeclaration><istex:docType PUBLIC="-//NLM//DTD Journal Publishing DTD v2.3 20070202//EN" URI="journalpublishing.dtd" name="istex:docType"></istex:docType><istex:document><article article-type="research-article"><front><journal-meta><journal-id journal-id-type="publisher-id">humrep</journal-id><journal-id journal-id-type="hwp">humrep</journal-id><journal-title>Human Reproduction</journal-title><issn pub-type="ppub">0268-1161</issn><issn pub-type="epub">1460-2350</issn><publisher><publisher-name>Oxford University Press</publisher-name></publisher></journal-meta><article-meta><article-id pub-id-type="doi">10.1093/humrep/dep005</article-id><article-id pub-id-type="publisher-id">dep005</article-id><article-categories><subj-group subj-group-type="heading"><subject>ORIGINAL ARTICLES</subject><subj-group><subject>Embryology</subject></subj-group></subj-group></article-categories><title-group><article-title>An <italic>in vivo</italic> culture system for human embryos using an encapsulation technology: a pilot study</article-title></title-group><contrib-group><contrib contrib-type="author"><name><surname>Blockeel</surname><given-names>C.</given-names></name><xref ref-type="aff" rid="af1">1</xref><xref ref-type="author-notes" rid="FN1">†</xref></contrib><contrib contrib-type="author"><name><surname>Mock</surname><given-names>P.</given-names></name><xref ref-type="aff" rid="af2">2</xref><xref ref-type="aff" rid="af9">9</xref><xref ref-type="author-notes" rid="FN1">†</xref></contrib><contrib contrib-type="author"><name><surname>Verheyen</surname><given-names>G.</given-names></name><xref ref-type="aff" rid="af1">1</xref><xref ref-type="author-notes" rid="FN1">†</xref></contrib><contrib contrib-type="author"><name><surname>Bouche</surname><given-names>N.</given-names></name><xref ref-type="aff" rid="af2">2</xref><xref ref-type="aff" rid="af3">3</xref></contrib><contrib contrib-type="author"><name><surname>Le Goff</surname><given-names>Ph.</given-names></name><xref ref-type="aff" rid="af2">2</xref></contrib><contrib contrib-type="author"><name><surname>Heyman</surname><given-names>Y.</given-names></name><xref ref-type="aff" rid="af4">4</xref></contrib><contrib contrib-type="author"><name><surname>Wrenzycki</surname><given-names>C.</given-names></name><xref ref-type="aff" rid="af5">5</xref></contrib><contrib contrib-type="author"><name><surname>Höffmann</surname><given-names>K.</given-names></name><xref ref-type="aff" rid="af5">5</xref></contrib><contrib contrib-type="author"><name><surname>Niemann</surname><given-names>H.</given-names></name><xref ref-type="aff" rid="af6">6</xref></contrib><contrib contrib-type="author"><name><surname>Haentjens</surname><given-names>P.</given-names></name><xref ref-type="aff" rid="af7">7</xref></contrib><contrib contrib-type="author"><name><surname>de Los Santos</surname><given-names>M.J.</given-names></name><xref ref-type="aff" rid="af8">8</xref></contrib><contrib contrib-type="author"><name><surname>Fernandez-Sanchez</surname><given-names>M.</given-names></name><xref ref-type="aff" rid="af8">8</xref></contrib><contrib contrib-type="author"><name><surname>Velasco</surname><given-names>M.</given-names></name><xref ref-type="aff" rid="af2">2</xref></contrib><contrib contrib-type="author"><name><surname>Aebischer</surname><given-names>P.</given-names></name><xref ref-type="aff" rid="af3">3</xref></contrib><contrib contrib-type="author"><name><surname>Devroey</surname><given-names>P.</given-names></name><xref ref-type="aff" rid="af1">1</xref></contrib><contrib contrib-type="author"><name><surname>Simón</surname><given-names>C.</given-names></name><xref ref-type="aff" rid="af8">8</xref><xref ref-type="corresp" rid="cor1">10</xref></contrib></contrib-group><aff id="af1"><label>1</label><institution>Centre for Reproductive Medicine</institution>, <addr-line>UZ Brussel, Brussels</addr-line>, <country>Belgium</country></aff><aff id="af2"><label>2</label><institution>Anecova Inc.</institution>, <addr-line>Lausanne</addr-line>, <country>Switzerland</country></aff><aff id="af3"><label>3</label><institution>Brain and Mind Institute, Ecole Polytechnique Fédérale de Lausanne, EPFL</institution>, <addr-line>Lausanne</addr-line>, <country>Switzerland</country></aff><aff id="af4"><label>4</label><institution>Institut National de Recherche Agronomique, INRA</institution>, <addr-line>Jouy en Josas</addr-line>, <country>France</country></aff><aff id="af5"><label>5</label><addr-line>Stiftung Tierärztliche Hochschule, Klinik für Rinder</addr-line>, <institution>Reproduktionsmedizinische Einheit der Kliniken</institution>, <addr-line>Hannover</addr-line>, <country>Germany</country></aff><aff id="af6"><label>6</label><institution>Institut für Nutztiergenetik, FLI, Mariensee</institution>, <addr-line>Neustadt</addr-line>, <country>Germany</country></aff><aff id="af7"><label>7</label><institution>Centre for Outcomes Research and Laboratory for Experimental Surgery</institution>, <addr-line>UZ Brussel, Brussels</addr-line>, <country>Belgium</country></aff><aff id="af8"><label>8</label><institution>Instituto Valenciano de Infertilidad (IVI)</institution>, <addr-line>Valencia</addr-line>, <country>Spain</country></aff><aff id="af9"><label>9</label><institution>University of Geneva</institution>, <addr-line>Geneva</addr-line>, <country>Switzerland</country></aff><author-notes><fn id="FN1"><label>†</label><p>The first three authors have contributed equally.</p></fn><corresp id="cor1"><label>10</label>Corresponding address. <institution>Fundación del Instituto Valenciano de Infertilidad (FIVI), University of Valencia</institution>, <addr-line>C/Guadassuar, 1, 46015 Valencia</addr-line>, <country>Spain</country>. Tel: <phone>+34-96-345-55-60</phone>; Fax: <fax>+34-96-345-55-12;</fax> E-mail: <email>csimon@ivi.es</email></corresp></author-notes><pub-date pub-type="ppub"><month>4</month><year>2009</year></pub-date><pub-date pub-type="epub"><day>10</day><month>3</month><year>2009</year></pub-date><volume>24</volume><issue>4</issue><fpage>790</fpage><lpage>796</lpage><history><date date-type="received"><day>2</day><month>6</month><year>2008</year></date><date date-type="rev-recd"><day>16</day><month>12</month><year>2008</year></date><date date-type="accepted"><day>28</day><month>12</month><year>2008</year></date></history><copyright-statement>© The Author 2009. Published by Oxford University Press on behalf of the European Society of Human Reproduction and Embryology. All rights reserved. For Permissions, please email: journals.permissions@oxfordjournals.org</copyright-statement><copyright-year>2009</copyright-year><license license-type="creative-commons" xlink:href="http://creativecommons.org/licenses/by-nc/2.0/uk/"><p>The online version of this article has been published under an open access model. Users are entitled to use, reproduce, disseminate, or display the open access version of this article for non-commercial purposes provided that: the original authorship is properly and fully attributed: the Journal and Oxford University Press are attributed as the original place of publication with the correct citation details given: if an article is subsequently reproduced or disseminated not in its entirety but only in part or as a derivative word this must be clearly indicated. For commercial re-use, please contact journals.permissions@oxfordjournals.org</p></license><abstract><sec><title>BACKGROUND</title><p>Animal studies have demonstrated better embryo development <italic>in vivo</italic> than <italic>in vitro</italic>. This pilot study tested the feasibility of using a novel <italic>in utero</italic> culture system (IUCS) to obtain normal human fertilization and embryo development.</p></sec><sec><title>METHODS</title><p>The IUCS device comprised a perforated silicone hollow tube. The study included 13 patients (<36 years) undergoing a first intracytoplasmic sperm injection (ICSI) treatment and 167 metaphase II oocytes in three groups. In Group 1, 1–2 h after ICSI, sibling oocytes were assigned to IUCS or conventional <italic>in vitro</italic> culture. The device was retrieved on Day 1, and all zygotes were cultured <italic>in vitro</italic> till Day 5. In Group 2, fertilized oocytes were assigned on Day 1, embryos retrieved on Day 3 and all embryos cultured till Day 5. In Group 3, after Day 0 assignment, embryos were retrieved on Day 3 for blastomere biopsy and fluorescence <italic>in situ</italic> hybridization (FISH) and cultured until Day 5. The highest quality blastocysts were transferred on Day 5.</p></sec><sec><title>RESULTS</title><p>Fertilization and embryo development were comparable in the <italic>in vitro</italic> and IUCS arms, with a tendency towards better embryo quality in the IUCS. FISH analysis in Group 3 revealed more normal embryos using the IUCS (<italic>P</italic> = 0.049). Three clinical pregnancies and live births were obtained: two from the IUCS arm and one from the <italic>in vitro</italic> arm.</p></sec><sec><title>CONCLUSIONS</title><p>Our pilot study shows that this new IUCS appears to be feasible and safe, supporting normal fertilization, embryo development and normal chromosomal segregation. Furthermore, live births are possible after the transient presence of a silicone device in the uterus.Clinicaltrials.gov: NCT00480103.</p></sec></abstract><kwd-group><title>Keywords</title><kwd><italic>in vivo</italic> culture</kwd><kwd><italic>in utero</italic> culture system</kwd><kwd>ICSI</kwd><kwd>embryos</kwd><kwd>oocytes</kwd></kwd-group></article-meta></front></article></istex:document></istex:metadataXml><mods version="3.6"><titleInfo><title>An in vivo culture system for human embryos using an encapsulation technology: a pilot study</title></titleInfo><titleInfo type="alternative" contentType="CDATA"><title>An in vivo culture system for human embryos using an encapsulation technology: a pilot study</title></titleInfo><name type="personal"><namePart type="given">C.</namePart><namePart type="family">Blockeel</namePart><affiliation>Centre for Reproductive Medicine, UZ Brussel, Brussels, Belgium</affiliation><description>The first three authors have contributed equally.</description><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">P.</namePart><namePart type="family">Mock</namePart><affiliation>Anecova Inc., Lausanne, Switzerland</affiliation><affiliation>University of Geneva, Geneva, Switzerland</affiliation><description>The first three authors have contributed equally.</description><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">G.</namePart><namePart type="family">Verheyen</namePart><affiliation>Centre for Reproductive Medicine, UZ Brussel, Brussels, Belgium</affiliation><description>The first three authors have contributed equally.</description><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">N.</namePart><namePart type="family">Bouche</namePart><affiliation>Anecova Inc., Lausanne, Switzerland</affiliation><affiliation>Brain and Mind Institute, Ecole Polytechnique Fdrale de Lausanne, EPFL, Lausanne, Switzerland</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">Ph.</namePart><namePart type="family">Le Goff</namePart><affiliation>Anecova Inc., Lausanne, Switzerland</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">Y.</namePart><namePart type="family">Heyman</namePart><affiliation>Institut National de Recherche Agronomique, INRA, Jouy en Josas, France</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">C.</namePart><namePart type="family">Wrenzycki</namePart><affiliation>Stiftung Tierrztliche Hochschule, Klinik fr Rinder, Reproduktionsmedizinische Einheit der Kliniken, Hannover, Germany</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">K.</namePart><namePart type="family">Hffmann</namePart><affiliation>Stiftung Tierrztliche Hochschule, Klinik fr Rinder, Reproduktionsmedizinische Einheit der Kliniken, Hannover, Germany</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">H.</namePart><namePart type="family">Niemann</namePart><affiliation>Institut fr Nutztiergenetik, FLI, Mariensee, Neustadt, Germany</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">P.</namePart><namePart type="family">Haentjens</namePart><affiliation>Centre for Outcomes Research and Laboratory for Experimental Surgery, UZ Brussel, Brussels, Belgium</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">M.J.</namePart><namePart type="family">de Los Santos</namePart><affiliation>Instituto Valenciano de Infertilidad (IVI), Valencia, Spain</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">M.</namePart><namePart type="family">Fernandez-Sanchez</namePart><affiliation>Instituto Valenciano de Infertilidad (IVI), Valencia, Spain</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">M.</namePart><namePart type="family">Velasco</namePart><affiliation>Anecova Inc., Lausanne, Switzerland</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">P.</namePart><namePart type="family">Aebischer</namePart><affiliation>Brain and Mind Institute, Ecole Polytechnique Fdrale de Lausanne, EPFL, Lausanne, Switzerland</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">P.</namePart><namePart type="family">Devroey</namePart><affiliation>Centre for Reproductive Medicine, UZ Brussel, Brussels, Belgium</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">C.</namePart><namePart type="family">Simn</namePart><affiliation>Instituto Valenciano de Infertilidad (IVI), Valencia, Spain</affiliation><affiliation>E-mail: csimon@ivi.es</affiliation><role><roleTerm type="text">author</roleTerm></role></name><typeOfResource>text</typeOfResource><genre type="research-article" displayLabel="research-article"></genre><originInfo><publisher>Oxford University Press</publisher><dateIssued encoding="w3cdtf">2009-04</dateIssued><dateCreated encoding="w3cdtf">2009-03-10</dateCreated><copyrightDate encoding="w3cdtf">2009</copyrightDate></originInfo><language><languageTerm type="code" authority="iso639-2b">eng</languageTerm><languageTerm type="code" authority="rfc3066">en</languageTerm></language><physicalDescription><internetMediaType>text/html</internetMediaType></physicalDescription><abstract>BACKGROUND Animal studies have demonstrated better embryo development in vivo than in vitro. This pilot study tested the feasibility of using a novel in utero culture system (IUCS) to obtain normal human fertilization and embryo development. METHODS The IUCS device comprised a perforated silicone hollow tube. The study included 13 patients (<36 years) undergoing a first intracytoplasmic sperm injection (ICSI) treatment and 167 metaphase II oocytes in three groups. In Group 1, 12 h after ICSI, sibling oocytes were assigned to IUCS or conventional in vitro culture. The device was retrieved on Day 1, and all zygotes were cultured in vitro till Day 5. In Group 2, fertilized oocytes were assigned on Day 1, embryos retrieved on Day 3 and all embryos cultured till Day 5. In Group 3, after Day 0 assignment, embryos were retrieved on Day 3 for blastomere biopsy and fluorescence in situ hybridization (FISH) and cultured until Day 5. The highest quality blastocysts were transferred on Day 5. RESULTS Fertilization and embryo development were comparable in the in vitro and IUCS arms, with a tendency towards better embryo quality in the IUCS. FISH analysis in Group 3 revealed more normal embryos using the IUCS (P 0.049). Three clinical pregnancies and live births were obtained: two from the IUCS arm and one from the in vitro arm. CONCLUSIONS Our pilot study shows that this new IUCS appears to be feasible and safe, supporting normal fertilization, embryo development and normal chromosomal segregation. Furthermore, live births are possible after the transient presence of a silicone device in the uterus.Clinicaltrials.gov: NCT00480103.</abstract><note type="footnotes">The first three authors have contributed equally.</note><subject><genre>Keywords</genre><topic>in vivo culture</topic><topic>in utero culture system</topic><topic>ICSI</topic><topic>embryos</topic><topic>oocytes</topic></subject><relatedItem type="host"><titleInfo><title>Human Reproduction</title></titleInfo><genre type="journal">journal</genre><identifier type="ISSN">0268-1161</identifier><identifier type="eISSN">1460-2350</identifier><identifier type="PublisherID">humrep</identifier><identifier type="PublisherID-hwp">humrep</identifier><part><date>2009</date><detail type="volume"><caption>vol.</caption><number>24</number></detail><detail type="issue"><caption>no.</caption><number>4</number></detail><extent unit="pages"><start>790</start><end>796</end></extent></part></relatedItem><identifier type="istex">033DA12265BEEFC4242E6536902363F8C6BE7EA5</identifier><identifier type="DOI">10.1093/humrep/dep005</identifier><identifier type="ArticleID">dep005</identifier><accessCondition type="use and reproduction" contentType="copyright">The Author 2009. 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