Investigation of the DNA-dependent cyclohexenyl nucleic acid polymerization and the cyclohexenyl nucleic acid-dependent DNA polymerization
Identifieur interne : 000A11 ( Istex/Corpus ); précédent : 000A10; suivant : 000A12Investigation of the DNA-dependent cyclohexenyl nucleic acid polymerization and the cyclohexenyl nucleic acid-dependent DNA polymerization
Auteurs : Veerle Kempeneers ; Marleen Renders ; Matheus Froeyen ; Piet HerdewijnSource :
- Nucleic Acids Research [ 0305-1048 ] ; 2005.
Abstract
DNA polymerases from different evolutionary families [Vent (exo−) DNA polymerase from the B-family polymerases, Taq DNA polymerase from the A-family polymerases and HIV reverse transcriptase from the reverse transcriptase family] were examined for their ability to incorporate the sugar-modified cyclohexenyl nucleoside triphosphates. All enzymes were able to use the cyclohexenyl nucleotides as a substrate. Using Vent (exo−) DNA polymerase and HIV reverse transcriptase, we were even able to incorporate seven consecutive cyclohexenyl nucleotides. Using a cyclohexenyl nucleic acid (CeNA) template, all enzymes tested were also able to synthesize a short DNA fragment. Since the DNA-dependent CeNA polymerization and the CeNA-dependent DNA polymerization is possible to a limited extend, we suggest CeNA as an ideal candidate to use in directed evolution methods for the development of a polymerase capable of replicating CeNA.
Url:
DOI: 10.1093/nar/gki695
Links to Exploration step
ISTEX:7EF3754DE94191D6CFD83BEA668242A7BE78D39FLe document en format XML
<record><TEI wicri:istexFullTextTei="biblStruct"><teiHeader><fileDesc><titleStmt><title xml:lang="en">Investigation of the DNA-dependent cyclohexenyl nucleic acid polymerization and the cyclohexenyl nucleic acid-dependent DNA polymerization</title><author><name sortKey="Kempeneers, Veerle" sort="Kempeneers, Veerle" uniqKey="Kempeneers V" first="Veerle" last="Kempeneers">Veerle Kempeneers</name><affiliation><mods:affiliation>Laboratory for Medicinal Chemistry, Rega Institute for Medical Research Minderbroedersstraat 10, B-3000 Leuven, Belgium</mods:affiliation></affiliation></author><author><name sortKey="Renders, Marleen" sort="Renders, Marleen" uniqKey="Renders M" first="Marleen" last="Renders">Marleen Renders</name><affiliation><mods:affiliation>Laboratory for Medicinal Chemistry, Rega Institute for Medical Research Minderbroedersstraat 10, B-3000 Leuven, Belgium</mods:affiliation></affiliation></author><author><name sortKey="Froeyen, Matheus" sort="Froeyen, Matheus" uniqKey="Froeyen M" first="Matheus" last="Froeyen">Matheus Froeyen</name><affiliation><mods:affiliation>Laboratory for Medicinal Chemistry, Rega Institute for Medical Research Minderbroedersstraat 10, B-3000 Leuven, Belgium</mods:affiliation></affiliation></author><author><name sortKey="Herdewijn, Piet" sort="Herdewijn, Piet" uniqKey="Herdewijn P" first="Piet" last="Herdewijn">Piet Herdewijn</name><affiliation><mods:affiliation>Laboratory for Medicinal Chemistry, Rega Institute for Medical Research Minderbroedersstraat 10, B-3000 Leuven, Belgium</mods:affiliation></affiliation></author></titleStmt><publicationStmt><idno type="wicri:source">ISTEX</idno><idno type="RBID">ISTEX:7EF3754DE94191D6CFD83BEA668242A7BE78D39F</idno><date when="2005" year="2005">2005</date><idno type="doi">10.1093/nar/gki695</idno><idno type="url">https://api.istex.fr/document/7EF3754DE94191D6CFD83BEA668242A7BE78D39F/fulltext/pdf</idno><idno type="wicri:Area/Istex/Corpus">000A11</idno></publicationStmt><sourceDesc><biblStruct><analytic><title level="a" type="main" xml:lang="en">Investigation of the DNA-dependent cyclohexenyl nucleic acid polymerization and the cyclohexenyl nucleic acid-dependent DNA polymerization</title><author><name sortKey="Kempeneers, Veerle" sort="Kempeneers, Veerle" uniqKey="Kempeneers V" first="Veerle" last="Kempeneers">Veerle Kempeneers</name><affiliation><mods:affiliation>Laboratory for Medicinal Chemistry, Rega Institute for Medical Research Minderbroedersstraat 10, B-3000 Leuven, Belgium</mods:affiliation></affiliation></author><author><name sortKey="Renders, Marleen" sort="Renders, Marleen" uniqKey="Renders M" first="Marleen" last="Renders">Marleen Renders</name><affiliation><mods:affiliation>Laboratory for Medicinal Chemistry, Rega Institute for Medical Research Minderbroedersstraat 10, B-3000 Leuven, Belgium</mods:affiliation></affiliation></author><author><name sortKey="Froeyen, Matheus" sort="Froeyen, Matheus" uniqKey="Froeyen M" first="Matheus" last="Froeyen">Matheus Froeyen</name><affiliation><mods:affiliation>Laboratory for Medicinal Chemistry, Rega Institute for Medical Research Minderbroedersstraat 10, B-3000 Leuven, Belgium</mods:affiliation></affiliation></author><author><name sortKey="Herdewijn, Piet" sort="Herdewijn, Piet" uniqKey="Herdewijn P" first="Piet" last="Herdewijn">Piet Herdewijn</name><affiliation><mods:affiliation>Laboratory for Medicinal Chemistry, Rega Institute for Medical Research Minderbroedersstraat 10, B-3000 Leuven, Belgium</mods:affiliation></affiliation></author></analytic><monogr></monogr><series><title level="j">Nucleic Acids Research</title><title level="j" type="abbrev">Nucl. Acids Res.</title><idno type="ISSN">0305-1048</idno><idno type="eISSN">1362-4962</idno><imprint><publisher>Oxford University Press</publisher><date type="published" when="2005">2005</date><biblScope unit="volume">33</biblScope><biblScope unit="issue">12</biblScope><biblScope unit="page" from="3828">3828</biblScope><biblScope unit="page" to="3836">3836</biblScope></imprint><idno type="ISSN">0305-1048</idno></series><idno type="istex">7EF3754DE94191D6CFD83BEA668242A7BE78D39F</idno><idno type="DOI">10.1093/nar/gki695</idno><idno type="local">gki695</idno></biblStruct></sourceDesc><seriesStmt><idno type="ISSN">0305-1048</idno></seriesStmt></fileDesc><profileDesc><textClass></textClass><langUsage><language ident="en">en</language></langUsage></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">DNA polymerases from different evolutionary families [Vent (exo−) DNA polymerase from the B-family polymerases, Taq DNA polymerase from the A-family polymerases and HIV reverse transcriptase from the reverse transcriptase family] were examined for their ability to incorporate the sugar-modified cyclohexenyl nucleoside triphosphates. All enzymes were able to use the cyclohexenyl nucleotides as a substrate. Using Vent (exo−) DNA polymerase and HIV reverse transcriptase, we were even able to incorporate seven consecutive cyclohexenyl nucleotides. Using a cyclohexenyl nucleic acid (CeNA) template, all enzymes tested were also able to synthesize a short DNA fragment. Since the DNA-dependent CeNA polymerization and the CeNA-dependent DNA polymerization is possible to a limited extend, we suggest CeNA as an ideal candidate to use in directed evolution methods for the development of a polymerase capable of replicating CeNA.</div></front></TEI><istex><corpusName>oup</corpusName><author><json:item><name>Veerle Kempeneers</name><affiliations><json:string>Laboratory for Medicinal Chemistry, Rega Institute for Medical Research Minderbroedersstraat 10, B-3000 Leuven, Belgium</json:string></affiliations></json:item><json:item><name>Marleen Renders</name><affiliations><json:string>Laboratory for Medicinal Chemistry, Rega Institute for Medical Research Minderbroedersstraat 10, B-3000 Leuven, Belgium</json:string></affiliations></json:item><json:item><name>Matheus Froeyen</name><affiliations><json:string>Laboratory for Medicinal Chemistry, Rega Institute for Medical Research Minderbroedersstraat 10, B-3000 Leuven, Belgium</json:string></affiliations></json:item><json:item><name>Piet Herdewijn</name><affiliations><json:string>Laboratory for Medicinal Chemistry, Rega Institute for Medical Research Minderbroedersstraat 10, B-3000 Leuven, Belgium</json:string><json:string>E-mail: Piet.Herdewijn@rega.kuleuven.ac.be</json:string></affiliations></json:item></author><language><json:string>eng</json:string></language><originalGenre><json:string>other</json:string></originalGenre><abstract>DNA polymerases from different evolutionary families [Vent (exo−) DNA polymerase from the B-family polymerases, Taq DNA polymerase from the A-family polymerases and HIV reverse transcriptase from the reverse transcriptase family] were examined for their ability to incorporate the sugar-modified cyclohexenyl nucleoside triphosphates. All enzymes were able to use the cyclohexenyl nucleotides as a substrate. Using Vent (exo−) DNA polymerase and HIV reverse transcriptase, we were even able to incorporate seven consecutive cyclohexenyl nucleotides. Using a cyclohexenyl nucleic acid (CeNA) template, all enzymes tested were also able to synthesize a short DNA fragment. Since the DNA-dependent CeNA polymerization and the CeNA-dependent DNA polymerization is possible to a limited extend, we suggest CeNA as an ideal candidate to use in directed evolution methods for the development of a polymerase capable of replicating CeNA.</abstract><qualityIndicators><score>7.072</score><pdfVersion>1.4</pdfVersion><pdfPageSize>612 x 794 pts</pdfPageSize><refBibsNative>false</refBibsNative><keywordCount>0</keywordCount><abstractCharCount>929</abstractCharCount><pdfWordCount>5859</pdfWordCount><pdfCharCount>38801</pdfCharCount><pdfPageCount>9</pdfPageCount><abstractWordCount>131</abstractWordCount></qualityIndicators><title>Investigation of the DNA-dependent cyclohexenyl nucleic acid polymerization and the cyclohexenyl nucleic acid-dependent DNA polymerization</title><genre><json:string>other</json:string></genre><host><volume>33</volume><publisherId><json:string>nar</json:string></publisherId><pages><last>3836</last><first>3828</first></pages><issn><json:string>0305-1048</json:string></issn><issue>12</issue><genre><json:string>journal</json:string></genre><language><json:string>unknown</json:string></language><eissn><json:string>1362-4962</json:string></eissn><title>Nucleic Acids Research</title></host><categories><wos><json:string>BIOCHEMISTRY & MOLECULAR BIOLOGY</json:string></wos></categories><publicationDate>2005</publicationDate><copyrightDate>2005</copyrightDate><doi><json:string>10.1093/nar/gki695</json:string></doi><id>7EF3754DE94191D6CFD83BEA668242A7BE78D39F</id><score>0.26687372</score><fulltext><json:item><original>true</original><mimetype>application/pdf</mimetype><extension>pdf</extension><uri>https://api.istex.fr/document/7EF3754DE94191D6CFD83BEA668242A7BE78D39F/fulltext/pdf</uri></json:item><json:item><original>false</original><mimetype>application/zip</mimetype><extension>zip</extension><uri>https://api.istex.fr/document/7EF3754DE94191D6CFD83BEA668242A7BE78D39F/fulltext/zip</uri></json:item><istex:fulltextTEI uri="https://api.istex.fr/document/7EF3754DE94191D6CFD83BEA668242A7BE78D39F/fulltext/tei"><teiHeader><fileDesc><titleStmt><title level="a" type="main" xml:lang="en">Investigation of the DNA-dependent cyclohexenyl nucleic acid polymerization and the cyclohexenyl nucleic acid-dependent DNA polymerization</title><respStmt><resp>Références bibliographiques récupérées via GROBID</resp><name resp="ISTEX-API">ISTEX-API (INIST-CNRS)</name></respStmt></titleStmt><publicationStmt><authority>ISTEX</authority><publisher>Oxford University Press</publisher><availability><p>© The Author 2005. Published by Oxford University Press. All rights reserved
The online version of this article has been published under an open access model. Users are entitled to use, reproduce, disseminate, or display the open access version of this article for non-commercial purposes provided that: the original authorship is properly and fully attributed; the Journal and Oxford University Press are attributed as the original place of publication with the correct citation details given; if an article is subsequently reproduced or disseminated not in its entirety but only in part or as a derivative work this must be clearly indicated. For commercial re-use, please contact journals.permissions@oupjournals.org</p></availability><date>2005</date></publicationStmt><notesStmt><note>*To whom correspondence should be addressed. Tel: +32 16 337387; Fax: +32 16 337340; Email: Piet.Herdewijn@rega.kuleuven.ac.be</note></notesStmt><sourceDesc><biblStruct type="inbook"><analytic><title level="a" type="main" xml:lang="en">Investigation of the DNA-dependent cyclohexenyl nucleic acid polymerization and the cyclohexenyl nucleic acid-dependent DNA polymerization</title><author xml:id="author-1"><persName><forename type="first">Veerle</forename><surname>Kempeneers</surname></persName><affiliation>Laboratory for Medicinal Chemistry, Rega Institute for Medical Research Minderbroedersstraat 10, B-3000 Leuven, Belgium</affiliation></author><author xml:id="author-2"><persName><forename type="first">Marleen</forename><surname>Renders</surname></persName><affiliation>Laboratory for Medicinal Chemistry, Rega Institute for Medical Research Minderbroedersstraat 10, B-3000 Leuven, Belgium</affiliation></author><author xml:id="author-3"><persName><forename type="first">Matheus</forename><surname>Froeyen</surname></persName><affiliation>Laboratory for Medicinal Chemistry, Rega Institute for Medical Research Minderbroedersstraat 10, B-3000 Leuven, Belgium</affiliation></author><author xml:id="author-4"><persName><forename type="first">Piet</forename><surname>Herdewijn</surname></persName><affiliation>Laboratory for Medicinal Chemistry, Rega Institute for Medical Research Minderbroedersstraat 10, B-3000 Leuven, Belgium</affiliation></author></analytic><monogr><title level="j">Nucleic Acids Research</title><title level="j" type="abbrev">Nucl. Acids Res.</title><idno type="pISSN">0305-1048</idno><idno type="eISSN">1362-4962</idno><imprint><publisher>Oxford University Press</publisher><date type="published" when="2005"></date><biblScope unit="volume">33</biblScope><biblScope unit="issue">12</biblScope><biblScope unit="page" from="3828">3828</biblScope><biblScope unit="page" to="3836">3836</biblScope></imprint></monogr><idno type="istex">7EF3754DE94191D6CFD83BEA668242A7BE78D39F</idno><idno type="DOI">10.1093/nar/gki695</idno><idno type="local">gki695</idno></biblStruct></sourceDesc></fileDesc><profileDesc><creation><date>2005</date></creation><langUsage><language ident="en">en</language></langUsage><abstract xml:lang="en"><p>DNA polymerases from different evolutionary families [Vent (exo−) DNA polymerase from the B-family polymerases, Taq DNA polymerase from the A-family polymerases and HIV reverse transcriptase from the reverse transcriptase family] were examined for their ability to incorporate the sugar-modified cyclohexenyl nucleoside triphosphates. All enzymes were able to use the cyclohexenyl nucleotides as a substrate. Using Vent (exo−) DNA polymerase and HIV reverse transcriptase, we were even able to incorporate seven consecutive cyclohexenyl nucleotides. Using a cyclohexenyl nucleic acid (CeNA) template, all enzymes tested were also able to synthesize a short DNA fragment. Since the DNA-dependent CeNA polymerization and the CeNA-dependent DNA polymerization is possible to a limited extend, we suggest CeNA as an ideal candidate to use in directed evolution methods for the development of a polymerase capable of replicating CeNA.</p></abstract></profileDesc><revisionDesc><change when="2005">Published</change><change xml:id="refBibs-istex" who="#ISTEX-API" when="2016-10-14">References added</change></revisionDesc></teiHeader></istex:fulltextTEI><json:item><original>false</original><mimetype>text/plain</mimetype><extension>txt</extension><uri>https://api.istex.fr/document/7EF3754DE94191D6CFD83BEA668242A7BE78D39F/fulltext/txt</uri></json:item></fulltext><metadata><istex:metadataXml wicri:clean="corpus oup" wicri:toSee="no header"><istex:xmlDeclaration>version="1.0" encoding="US-ASCII"</istex:xmlDeclaration><istex:docType PUBLIC="-//NLM//DTD Journal Publishing DTD v2.3 20070202//EN" URI="journalpublishing.dtd" name="istex:docType"></istex:docType><istex:document><article xml:lang="en" article-type="other"><front><journal-meta><journal-id journal-id-type="hwp">nar</journal-id><journal-id journal-id-type="nlm-ta">Nucleic Acids Res</journal-id><journal-id journal-id-type="publisher-id">nar</journal-id><journal-title>Nucleic Acids Research</journal-title><abbrev-journal-title abbrev-type="publisher">Nucl. Acids Res.</abbrev-journal-title><issn pub-type="ppub">0305-1048</issn><issn pub-type="epub">1362-4962</issn><publisher><publisher-name>Oxford University Press</publisher-name></publisher></journal-meta><article-meta><article-id pub-id-type="other">gki695</article-id><article-id pub-id-type="doi">10.1093/nar/gki695</article-id><article-categories><subj-group subj-group-type="heading"><subject>Article</subject></subj-group></article-categories><title-group><article-title>Investigation of the DNA-dependent cyclohexenyl nucleic acid polymerization and the cyclohexenyl nucleic acid-dependent DNA polymerization</article-title></title-group><contrib-group><contrib contrib-type="author"><name><surname>Kempeneers</surname><given-names>Veerle</given-names></name></contrib><contrib contrib-type="author"><name><surname>Renders</surname><given-names>Marleen</given-names></name></contrib><contrib contrib-type="author"><name><surname>Froeyen</surname><given-names>Matheus</given-names></name></contrib><contrib contrib-type="author"><name><surname>Herdewijn</surname><given-names>Piet</given-names></name><xref rid="COR1">*</xref></contrib><aff>Laboratory for Medicinal Chemistry, Rega Institute for Medical Research Minderbroedersstraat 10, B-3000 Leuven, Belgium</aff></contrib-group><author-notes><corresp id="COR1"><sup>*</sup>To whom correspondence should be addressed. Tel: +32 16 337387; Fax: +32 16 337340; Email: <ext-link xlink:href="Piet.Herdewijn@rega.kuleuven.ac.be" ext-link-type="email">Piet.Herdewijn@rega.kuleuven.ac.be</ext-link></corresp></author-notes><pub-date pub-type="ppub"><year>2005</year></pub-date><volume>33</volume><issue>12</issue><fpage>3828</fpage><lpage>3836</lpage><history><date date-type="accepted"><day>17</day><month>6</month><year>2005</year></date><date date-type="received"><day>11</day><month>4</month><year>2005</year></date><date date-type="rev-recd"><day>27</day><month>5</month><year>2005</year></date></history><permissions><copyright-statement>© The Author 2005. Published by Oxford University Press. All rights reserved
The online version of this article has been published under an open access model. Users are entitled to use, reproduce, disseminate, or display the open access version of this article for non-commercial purposes provided that: the original authorship is properly and fully attributed; the Journal and Oxford University Press are attributed as the original place of publication with the correct citation details given; if an article is subsequently reproduced or disseminated not in its entirety but only in part or as a derivative work this must be clearly indicated. For commercial re-use, please contact <ext-link xlink:href="journals.permissions@oupjournals.org" ext-link-type="email">journals.permissions@oupjournals.org</ext-link></copyright-statement><copyright-year>2005</copyright-year><license license-type="open-access"><p></p></license></permissions><abstract xml:lang="en"><p>DNA polymerases from different evolutionary families [Vent (exo−) DNA polymerase from the B-family polymerases, <italic>Taq</italic> DNA polymerase from the A-family polymerases and HIV reverse transcriptase from the reverse transcriptase family] were examined for their ability to incorporate the sugar-modified cyclohexenyl nucleoside triphosphates. All enzymes were able to use the cyclohexenyl nucleotides as a substrate. Using Vent (exo−) DNA polymerase and HIV reverse transcriptase, we were even able to incorporate seven consecutive cyclohexenyl nucleotides. Using a cyclohexenyl nucleic acid (CeNA) template, all enzymes tested were also able to synthesize a short DNA fragment. Since the DNA-dependent CeNA polymerization and the CeNA-dependent DNA polymerization is possible to a limited extend, we suggest CeNA as an ideal candidate to use in directed evolution methods for the development of a polymerase capable of replicating CeNA.</p></abstract><custom-meta-wrap><custom-meta><meta-name>hwp-legacy-fpage</meta-name><meta-value>3828</meta-value></custom-meta><custom-meta><meta-name>cover-date</meta-name><meta-value></meta-value></custom-meta><custom-meta><meta-name>hwp-legacy-dochead</meta-name><meta-value>research-article</meta-value></custom-meta></custom-meta-wrap></article-meta></front></article></istex:document></istex:metadataXml><mods version="3.6"><titleInfo lang="en"><title>Investigation of the DNA-dependent cyclohexenyl nucleic acid polymerization and the cyclohexenyl nucleic acid-dependent DNA polymerization</title></titleInfo><titleInfo type="alternative" lang="en" contentType="CDATA"><title>Investigation of the DNA-dependent cyclohexenyl nucleic acid polymerization and the cyclohexenyl nucleic acid-dependent DNA polymerization</title></titleInfo><name type="personal"><namePart type="given">Veerle</namePart><namePart type="family">Kempeneers</namePart><affiliation>Laboratory for Medicinal Chemistry, Rega Institute for Medical Research Minderbroedersstraat 10, B-3000 Leuven, Belgium</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">Marleen</namePart><namePart type="family">Renders</namePart><affiliation>Laboratory for Medicinal Chemistry, Rega Institute for Medical Research Minderbroedersstraat 10, B-3000 Leuven, Belgium</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">Matheus</namePart><namePart type="family">Froeyen</namePart><affiliation>Laboratory for Medicinal Chemistry, Rega Institute for Medical Research Minderbroedersstraat 10, B-3000 Leuven, Belgium</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">Piet</namePart><namePart type="family">Herdewijn</namePart><affiliation>Laboratory for Medicinal Chemistry, Rega Institute for Medical Research Minderbroedersstraat 10, B-3000 Leuven, Belgium</affiliation><role><roleTerm type="text">author</roleTerm></role></name><typeOfResource>text</typeOfResource><genre type="other" displayLabel="other"></genre><originInfo><publisher>Oxford University Press</publisher><dateIssued encoding="w3cdtf">2005</dateIssued><copyrightDate encoding="w3cdtf">2005</copyrightDate></originInfo><language><languageTerm type="code" authority="iso639-2b">eng</languageTerm><languageTerm type="code" authority="rfc3066">en</languageTerm></language><physicalDescription><internetMediaType>text/html</internetMediaType></physicalDescription><abstract lang="en">DNA polymerases from different evolutionary families [Vent (exo−) DNA polymerase from the B-family polymerases, Taq DNA polymerase from the A-family polymerases and HIV reverse transcriptase from the reverse transcriptase family] were examined for their ability to incorporate the sugar-modified cyclohexenyl nucleoside triphosphates. All enzymes were able to use the cyclohexenyl nucleotides as a substrate. Using Vent (exo−) DNA polymerase and HIV reverse transcriptase, we were even able to incorporate seven consecutive cyclohexenyl nucleotides. Using a cyclohexenyl nucleic acid (CeNA) template, all enzymes tested were also able to synthesize a short DNA fragment. Since the DNA-dependent CeNA polymerization and the CeNA-dependent DNA polymerization is possible to a limited extend, we suggest CeNA as an ideal candidate to use in directed evolution methods for the development of a polymerase capable of replicating CeNA.</abstract><note type="author-notes">*To whom correspondence should be addressed. Tel: +32 16 337387; Fax: +32 16 337340; Email: Piet.Herdewijn@rega.kuleuven.ac.be</note><relatedItem type="host"><titleInfo><title>Nucleic Acids Research</title></titleInfo><titleInfo type="abbreviated"><title>Nucl. Acids Res.</title></titleInfo><genre type="journal">journal</genre><identifier type="ISSN">0305-1048</identifier><identifier type="eISSN">1362-4962</identifier><identifier type="PublisherID">nar</identifier><identifier type="PublisherID-hwp">nar</identifier><identifier type="PublisherID-nlm-ta">Nucleic Acids Res</identifier><part><date>2005</date><detail type="volume"><caption>vol.</caption><number>33</number></detail><detail type="issue"><caption>no.</caption><number>12</number></detail><extent unit="pages"><start>3828</start><end>3836</end></extent></part></relatedItem><identifier type="istex">7EF3754DE94191D6CFD83BEA668242A7BE78D39F</identifier><identifier type="DOI">10.1093/nar/gki695</identifier><identifier type="local">gki695</identifier><accessCondition type="use and reproduction" contentType="copyright">© The Author 2005. Published by Oxford University Press. All rights reserved
The online version of this article has been published under an open access model. Users are entitled to use, reproduce, disseminate, or display the open access version of this article for non-commercial purposes provided that: the original authorship is properly and fully attributed; the Journal and Oxford University Press are attributed as the original place of publication with the correct citation details given; if an article is subsequently reproduced or disseminated not in its entirety but only in part or as a derivative work this must be clearly indicated. For commercial re-use, please contact journals.permissions@oupjournals.org</accessCondition><recordInfo><recordContentSource>OUP</recordContentSource></recordInfo></mods></metadata><annexes><json:item><original>true</original><mimetype>image/jpeg</mimetype><extension>jpeg</extension><uri>https://api.istex.fr/document/7EF3754DE94191D6CFD83BEA668242A7BE78D39F/annexes/jpeg</uri></json:item><json:item><original>true</original><mimetype>image/gif</mimetype><extension>gif</extension><uri>https://api.istex.fr/document/7EF3754DE94191D6CFD83BEA668242A7BE78D39F/annexes/gif</uri></json:item></annexes><enrichments><istex:catWosTEI uri="https://api.istex.fr/document/7EF3754DE94191D6CFD83BEA668242A7BE78D39F/enrichments/catWos"><teiHeader><profileDesc><textClass><classCode scheme="WOS">BIOCHEMISTRY & MOLECULAR BIOLOGY</classCode></textClass></profileDesc></teiHeader></istex:catWosTEI><json:item><type>refBibs</type><uri>https://api.istex.fr/document/7EF3754DE94191D6CFD83BEA668242A7BE78D39F/enrichments/refBibs</uri></json:item></enrichments><serie></serie></istex></record>Pour manipuler ce document sous Unix (Dilib)
EXPLOR_STEP=$WICRI_ROOT/Wicri/Belgique/explor/OpenAccessBelV2/Data/Istex/Corpus
HfdSelect -h $EXPLOR_STEP/biblio.hfd -nk 000A11 | SxmlIndent | more
Ou
HfdSelect -h $EXPLOR_AREA/Data/Istex/Corpus/biblio.hfd -nk 000A11 | SxmlIndent | more
Pour mettre un lien sur cette page dans le réseau Wicri
{{Explor lien
|wiki= Wicri/Belgique
|area= OpenAccessBelV2
|flux= Istex
|étape= Corpus
|type= RBID
|clé= ISTEX:7EF3754DE94191D6CFD83BEA668242A7BE78D39F
|texte= Investigation of the DNA-dependent cyclohexenyl nucleic acid polymerization and the cyclohexenyl nucleic acid-dependent DNA polymerization
}}
| This area was generated with Dilib version V0.6.25. |  |