Insight into the prebiotic concept: lessons from an exploratory, double blind intervention study with inulin-type fructans in obese women
Identifieur interne : 000789 ( Istex/Corpus ); précédent : 000788; suivant : 000790Insight into the prebiotic concept: lessons from an exploratory, double blind intervention study with inulin-type fructans in obese women
Auteurs : Evelyne M. Dewulf ; Patrice D. Cani ; Sandrine P. Claus ; Susana Fuentes ; Philippe Gb Puylaert ; Audrey M. Neyrinck ; Laure B. Bindels ; Willem M. De Vos ; Glenn R. Gibson ; Jean-Paul Thissen ; Nathalie M. DelzenneSource :
- Gut [ 0017-5749 ] ; 2013-08.
Abstract
Objective To highlight the contribution of the gut microbiota to the modulation of host metabolism by dietary inulin-type fructans (ITF prebiotics) in obese women. Methods A double blind, placebo controlled, intervention study was performed with 30 obese women treated with ITF prebiotics (inulin/oligofructose 50/50 mix; n=15) or placebo (maltodextrin; n=15) for 3 months (16 g/day). Blood, faeces and urine sampling, oral glucose tolerance test, homeostasis model assessment and impedancemetry were performed before and after treatment. The gut microbial composition in faeces was analysed by phylogenetic microarray and qPCR analysis of 16S rDNA. Plasma and urine metabolic profiles were analysed by 1H-NMR spectroscopy. Results Treatment with ITF prebiotics, but not the placebo, led to an increase in Bifidobacterium and Faecalibacterium prausnitzii; both bacteria negatively correlated with serum lipopolysaccharide levels. ITF prebiotics also decreased Bacteroides intestinalis, Bacteroides vulgatus and Propionibacterium, an effect associated with a slight decrease in fat mass and with plasma lactate and phosphatidylcholine levels. No clear treatment clustering could be detected for gut microbial analysis or plasma and urine metabolomic profile analyses. However, ITF prebiotics led to subtle changes in the gut microbiota that may importantly impact on several key metabolites implicated in obesity and/or diabetes. Conclusions ITF prebiotics selectively changed the gut microbiota composition in obese women, leading to modest changes in host metabolism, as suggested by the correlation between some bacterial species and metabolic endotoxaemia or metabolomic signatures.
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DOI: 10.1136/gutjnl-2012-303304
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Neyrinck</name><affiliation><mods:affiliation>Metabolism and Nutrition Research Group, LDRI, Université catholique de Louvain, Bruxelles, Belgium</mods:affiliation></affiliation></author><author><name sortKey="Bindels, Laure B" sort="Bindels, Laure B" uniqKey="Bindels L" first="Laure B" last="Bindels">Laure B. Bindels</name><affiliation><mods:affiliation>Metabolism and Nutrition Research Group, LDRI, Université catholique de Louvain, Bruxelles, Belgium</mods:affiliation></affiliation></author><author><name sortKey="De Vos, Willem M" sort="De Vos, Willem M" uniqKey="De Vos W" first="Willem M" last="De Vos">Willem M. De Vos</name><affiliation><mods:affiliation>Laboratory of Microbiology, Wageningen University and Research Centre, Wageningen, The Netherlands</mods:affiliation></affiliation></author><author><name sortKey="Gibson, Glenn R" sort="Gibson, Glenn R" uniqKey="Gibson G" first="Glenn R" last="Gibson">Glenn R. 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Dewulf</name><affiliation><mods:affiliation>Metabolism and Nutrition Research Group, LDRI, Université catholique de Louvain, Bruxelles, Belgium</mods:affiliation></affiliation></author><author><name sortKey="Cani, Patrice D" sort="Cani, Patrice D" uniqKey="Cani P" first="Patrice D" last="Cani">Patrice D. Cani</name><affiliation><mods:affiliation>Metabolism and Nutrition Research Group, LDRI, Université catholique de Louvain, Bruxelles, Belgium</mods:affiliation></affiliation></author><author><name sortKey="Claus, Sandrine P" sort="Claus, Sandrine P" uniqKey="Claus S" first="Sandrine P" last="Claus">Sandrine P. Claus</name><affiliation><mods:affiliation>Department of Food and Nutritional Sciences, University of Reading, Reading, UK</mods:affiliation></affiliation></author><author><name sortKey="Fuentes, Susana" sort="Fuentes, Susana" uniqKey="Fuentes S" first="Susana" last="Fuentes">Susana Fuentes</name><affiliation><mods:affiliation>Laboratory of Microbiology, Wageningen University and Research Centre, Wageningen, The Netherlands</mods:affiliation></affiliation></author><author><name sortKey="Puylaert, Philippe Gb" sort="Puylaert, Philippe Gb" uniqKey="Puylaert P" first="Philippe Gb" last="Puylaert">Philippe Gb Puylaert</name><affiliation><mods:affiliation>Laboratory of Microbiology, Wageningen University and Research Centre, Wageningen, The Netherlands</mods:affiliation></affiliation></author><author><name sortKey="Neyrinck, Audrey M" sort="Neyrinck, Audrey M" uniqKey="Neyrinck A" first="Audrey M" last="Neyrinck">Audrey M. Neyrinck</name><affiliation><mods:affiliation>Metabolism and Nutrition Research Group, LDRI, Université catholique de Louvain, Bruxelles, Belgium</mods:affiliation></affiliation></author><author><name sortKey="Bindels, Laure B" sort="Bindels, Laure B" uniqKey="Bindels L" first="Laure B" last="Bindels">Laure B. Bindels</name><affiliation><mods:affiliation>Metabolism and Nutrition Research Group, LDRI, Université catholique de Louvain, Bruxelles, Belgium</mods:affiliation></affiliation></author><author><name sortKey="De Vos, Willem M" sort="De Vos, Willem M" uniqKey="De Vos W" first="Willem M" last="De Vos">Willem M. De Vos</name><affiliation><mods:affiliation>Laboratory of Microbiology, Wageningen University and Research Centre, Wageningen, The Netherlands</mods:affiliation></affiliation></author><author><name sortKey="Gibson, Glenn R" sort="Gibson, Glenn R" uniqKey="Gibson G" first="Glenn R" last="Gibson">Glenn R. Gibson</name><affiliation><mods:affiliation>Department of Food and Nutritional Sciences, University of Reading, Reading, UK</mods:affiliation></affiliation></author><author><name sortKey="Thissen, Jean Paul" sort="Thissen, Jean Paul" uniqKey="Thissen J" first="Jean-Paul" last="Thissen">Jean-Paul Thissen</name><affiliation><mods:affiliation>Pole of Endocrinology, Diabetology and Nutrition, IREC, Université catholique de Louvain, Bruxelles, Belgium</mods:affiliation></affiliation></author><author><name sortKey="Delzenne, Nathalie M" sort="Delzenne, Nathalie M" uniqKey="Delzenne N" first="Nathalie M" last="Delzenne">Nathalie M. Delzenne</name><affiliation><mods:affiliation>Metabolism and Nutrition Research Group, LDRI, Université catholique de Louvain, Bruxelles, Belgium</mods:affiliation></affiliation></author></analytic><monogr></monogr><series><title level="j">Gut</title><title level="j" type="abbrev">Gut</title><idno type="ISSN">0017-5749</idno><idno type="eISSN">1468-3288</idno><imprint><publisher>BMJ Publishing Group Ltd and British Society of Gastroenterology</publisher><date type="published" when="2013-08">2013-08</date><biblScope unit="volume">62</biblScope><biblScope unit="issue">8</biblScope><biblScope unit="page" from="1112">1112</biblScope></imprint><idno type="ISSN">0017-5749</idno></series><idno type="istex">E750762DD9B4B139E4D5AB53F3CCB6D48AB1D2CE</idno><idno type="DOI">10.1136/gutjnl-2012-303304</idno><idno type="href">gutjnl-62-1112.pdf</idno><idno type="ArticleID">gutjnl-2012-303304</idno><idno type="PMID">23135760</idno><idno type="Related-article-href">10.1136/gutjnl-2012-303908</idno><idno type="Related-article-href">10.1136/gutjnl-2012-304393</idno><idno type="related-article-ID">RA1</idno><idno type="local">gutjnl;62/8/1112</idno></biblStruct></sourceDesc><seriesStmt><idno type="ISSN">0017-5749</idno></seriesStmt></fileDesc><profileDesc><textClass></textClass><langUsage><language ident="en">en</language></langUsage></profileDesc></teiHeader><front><div type="abstract">Objective To highlight the contribution of the gut microbiota to the modulation of host metabolism by dietary inulin-type fructans (ITF prebiotics) in obese women. Methods A double blind, placebo controlled, intervention study was performed with 30 obese women treated with ITF prebiotics (inulin/oligofructose 50/50 mix; n=15) or placebo (maltodextrin; n=15) for 3 months (16 g/day). Blood, faeces and urine sampling, oral glucose tolerance test, homeostasis model assessment and impedancemetry were performed before and after treatment. The gut microbial composition in faeces was analysed by phylogenetic microarray and qPCR analysis of 16S rDNA. Plasma and urine metabolic profiles were analysed by 1H-NMR spectroscopy. Results Treatment with ITF prebiotics, but not the placebo, led to an increase in Bifidobacterium and Faecalibacterium prausnitzii; both bacteria negatively correlated with serum lipopolysaccharide levels. ITF prebiotics also decreased Bacteroides intestinalis, Bacteroides vulgatus and Propionibacterium, an effect associated with a slight decrease in fat mass and with plasma lactate and phosphatidylcholine levels. No clear treatment clustering could be detected for gut microbial analysis or plasma and urine metabolomic profile analyses. However, ITF prebiotics led to subtle changes in the gut microbiota that may importantly impact on several key metabolites implicated in obesity and/or diabetes. Conclusions ITF prebiotics selectively changed the gut microbiota composition in obese women, leading to modest changes in host metabolism, as suggested by the correlation between some bacterial species and metabolic endotoxaemia or metabolomic signatures.</div></front></TEI><istex><corpusName>bmj</corpusName><author><json:item><name>Evelyne M Dewulf</name><affiliations><json:string>Metabolism and Nutrition Research Group, LDRI, Université catholique de Louvain, Bruxelles, Belgium</json:string></affiliations></json:item><json:item><name>Patrice D Cani</name><affiliations><json:string>Metabolism and Nutrition Research Group, LDRI, Université catholique de Louvain, Bruxelles, Belgium</json:string></affiliations></json:item><json:item><name>Sandrine P Claus</name><affiliations><json:string>Department of Food and Nutritional Sciences, University of Reading, Reading, UK</json:string></affiliations></json:item><json:item><name>Susana Fuentes</name><affiliations><json:string>Laboratory of Microbiology, Wageningen University and Research Centre, Wageningen, The Netherlands</json:string></affiliations></json:item><json:item><name>Philippe GB Puylaert</name><affiliations><json:string>Laboratory of Microbiology, Wageningen University and Research Centre, Wageningen, The Netherlands</json:string></affiliations></json:item><json:item><name>Audrey M Neyrinck</name><affiliations><json:string>Metabolism and Nutrition Research Group, LDRI, Université catholique de Louvain, Bruxelles, Belgium</json:string></affiliations></json:item><json:item><name>Laure B Bindels</name><affiliations><json:string>Metabolism and Nutrition Research Group, LDRI, Université catholique de Louvain, Bruxelles, Belgium</json:string></affiliations></json:item><json:item><name>Willem M de Vos</name><affiliations><json:string>Laboratory of Microbiology, Wageningen University and Research Centre, Wageningen, The Netherlands</json:string></affiliations></json:item><json:item><name>Glenn R Gibson</name><affiliations><json:string>Department of Food and Nutritional Sciences, University of Reading, Reading, UK</json:string></affiliations></json:item><json:item><name>Jean-Paul Thissen</name><affiliations><json:string>Pole of Endocrinology, Diabetology and Nutrition, IREC, Université catholique de Louvain, Bruxelles, Belgium</json:string></affiliations></json:item><json:item><name>Nathalie M Delzenne</name><affiliations><json:string>Metabolism and Nutrition Research Group, LDRI, Université catholique de Louvain, Bruxelles, Belgium</json:string></affiliations></json:item></author><subject><json:item><lang><json:string>eng</json:string></lang><value>Open access</value></json:item><json:item><lang><json:string>eng</json:string></lang><value>Obesity</value></json:item><json:item><lang><json:string>eng</json:string></lang><value>Prebiotic</value></json:item><json:item><lang><json:string>eng</json:string></lang><value>Nutrition</value></json:item><json:item><lang><json:string>eng</json:string></lang><value>Intestinal Bacteria</value></json:item></subject><articleId><json:string>gutjnl-2012-303304</json:string></articleId><language><json:string>eng</json:string></language><originalGenre><json:string>research-article</json:string></originalGenre><abstract>Objective To highlight the contribution of the gut microbiota to the modulation of host metabolism by dietary inulin-type fructans (ITF prebiotics) in obese women. Methods A double blind, placebo controlled, intervention study was performed with 30 obese women treated with ITF prebiotics (inulin/oligofructose 50/50 mix; n=15) or placebo (maltodextrin; n=15) for 3 months (16 g/day). Blood, faeces and urine sampling, oral glucose tolerance test, homeostasis model assessment and impedancemetry were performed before and after treatment. The gut microbial composition in faeces was analysed by phylogenetic microarray and qPCR analysis of 16S rDNA. Plasma and urine metabolic profiles were analysed by 1H-NMR spectroscopy. Results Treatment with ITF prebiotics, but not the placebo, led to an increase in Bifidobacterium and Faecalibacterium prausnitzii; both bacteria negatively correlated with serum lipopolysaccharide levels. ITF prebiotics also decreased Bacteroides intestinalis, Bacteroides vulgatus and Propionibacterium, an effect associated with a slight decrease in fat mass and with plasma lactate and phosphatidylcholine levels. No clear treatment clustering could be detected for gut microbial analysis or plasma and urine metabolomic profile analyses. However, ITF prebiotics led to subtle changes in the gut microbiota that may importantly impact on several key metabolites implicated in obesity and/or diabetes. Conclusions ITF prebiotics selectively changed the gut microbiota composition in obese women, leading to modest changes in host metabolism, as suggested by the correlation between some bacterial species and metabolic endotoxaemia or metabolomic signatures.</abstract><qualityIndicators><score>7.748</score><pdfVersion>1.3</pdfVersion><pdfPageSize>595.276 x 793.701 pts</pdfPageSize><refBibsNative>true</refBibsNative><keywordCount>5</keywordCount><abstractCharCount>1685</abstractCharCount><pdfWordCount>6155</pdfWordCount><pdfCharCount>42125</pdfCharCount><pdfPageCount>10</pdfPageCount><abstractWordCount>229</abstractWordCount></qualityIndicators><title>Insight into the prebiotic concept: lessons from an exploratory, double blind intervention study with inulin-type fructans in obese women</title><pmid><json:string>23135760</json:string></pmid><genre><json:string>research-article</json:string></genre><host><volume>62</volume><publisherId><json:string>gut</json:string></publisherId><pages><first>1112</first></pages><issn><json:string>0017-5749</json:string></issn><issue>8</issue><genre><json:string>journal</json:string></genre><language><json:string>unknown</json:string></language><eissn><json:string>1468-3288</json:string></eissn><title>Gut</title></host><publicationDate>2013</publicationDate><copyrightDate>2013</copyrightDate><doi><json:string>10.1136/gutjnl-2012-303304</json:string></doi><id>E750762DD9B4B139E4D5AB53F3CCB6D48AB1D2CE</id><score>0.28290954</score><fulltext><json:item><original>true</original><mimetype>application/pdf</mimetype><extension>pdf</extension><uri>https://api.istex.fr/document/E750762DD9B4B139E4D5AB53F3CCB6D48AB1D2CE/fulltext/pdf</uri></json:item><json:item><original>false</original><mimetype>application/zip</mimetype><extension>zip</extension><uri>https://api.istex.fr/document/E750762DD9B4B139E4D5AB53F3CCB6D48AB1D2CE/fulltext/zip</uri></json:item><istex:fulltextTEI 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Wageningen, The Netherlands</affiliation></author><author xml:id="author-6"><persName><forename type="first">Audrey M</forename><surname>Neyrinck</surname></persName><affiliation>Metabolism and Nutrition Research Group, LDRI, Université catholique de Louvain, Bruxelles, Belgium</affiliation></author><author xml:id="author-7"><persName><forename type="first">Laure B</forename><surname>Bindels</surname></persName><affiliation>Metabolism and Nutrition Research Group, LDRI, Université catholique de Louvain, Bruxelles, Belgium</affiliation></author><author xml:id="author-8"><persName><forename type="first">Willem M</forename><surname>de Vos</surname></persName><affiliation>Laboratory of Microbiology, Wageningen University and Research Centre, Wageningen, The Netherlands</affiliation></author><author xml:id="author-9"><persName><forename type="first">Glenn R</forename><surname>Gibson</surname></persName><affiliation>Department of Food and Nutritional Sciences, University of Reading, Reading, UK</affiliation></author><author xml:id="author-10"><persName><forename type="first">Jean-Paul</forename><surname>Thissen</surname></persName><affiliation>Pole of Endocrinology, Diabetology and Nutrition, IREC, Université catholique de Louvain, Bruxelles, Belgium</affiliation></author><author xml:id="author-11"><persName><forename type="first">Nathalie M</forename><surname>Delzenne</surname></persName><affiliation>Metabolism and Nutrition Research Group, LDRI, Université catholique de Louvain, Bruxelles, Belgium</affiliation></author></analytic><monogr><title level="j">Gut</title><title level="j" type="abbrev">Gut</title><idno type="pISSN">0017-5749</idno><idno type="eISSN">1468-3288</idno><imprint><publisher>BMJ Publishing Group Ltd and British Society of Gastroenterology</publisher><date type="published" when="2013-08"></date><biblScope unit="volume">62</biblScope><biblScope unit="issue">8</biblScope><biblScope unit="page" from="1112">1112</biblScope></imprint></monogr><idno type="istex">E750762DD9B4B139E4D5AB53F3CCB6D48AB1D2CE</idno><idno type="DOI">10.1136/gutjnl-2012-303304</idno><idno type="href">gutjnl-62-1112.pdf</idno><idno type="ArticleID">gutjnl-2012-303304</idno><idno type="PMID">23135760</idno><idno type="Related-article-href">10.1136/gutjnl-2012-303908</idno><idno type="Related-article-href">10.1136/gutjnl-2012-304393</idno><idno type="related-article-ID">RA1</idno><idno type="local">gutjnl;62/8/1112</idno></biblStruct></sourceDesc></fileDesc><profileDesc><creation><date>2012-11-07</date></creation><langUsage><language ident="en">en</language></langUsage><abstract><p>Objective To highlight the contribution of the gut microbiota to the modulation of host metabolism by dietary inulin-type fructans (ITF prebiotics) in obese women. Methods A double blind, placebo controlled, intervention study was performed with 30 obese women treated with ITF prebiotics (inulin/oligofructose 50/50 mix; n=15) or placebo (maltodextrin; n=15) for 3 months (16 g/day). Blood, faeces and urine sampling, oral glucose tolerance test, homeostasis model assessment and impedancemetry were performed before and after treatment. The gut microbial composition in faeces was analysed by phylogenetic microarray and qPCR analysis of 16S rDNA. Plasma and urine metabolic profiles were analysed by 1H-NMR spectroscopy. Results Treatment with ITF prebiotics, but not the placebo, led to an increase in Bifidobacterium and Faecalibacterium prausnitzii; both bacteria negatively correlated with serum lipopolysaccharide levels. ITF prebiotics also decreased Bacteroides intestinalis, Bacteroides vulgatus and Propionibacterium, an effect associated with a slight decrease in fat mass and with plasma lactate and phosphatidylcholine levels. No clear treatment clustering could be detected for gut microbial analysis or plasma and urine metabolomic profile analyses. However, ITF prebiotics led to subtle changes in the gut microbiota that may importantly impact on several key metabolites implicated in obesity and/or diabetes. Conclusions ITF prebiotics selectively changed the gut microbiota composition in obese women, leading to modest changes in host metabolism, as suggested by the correlation between some bacterial species and metabolic endotoxaemia or metabolomic signatures.</p></abstract><textClass><keywords scheme="keyword"><list><head>hwp-journal-coll</head><item><term>Open access</term></item></list></keywords></textClass><textClass><keywords scheme="keyword"><list><head>keywords</head><item><term>Obesity</term></item><item><term>Prebiotic</term></item><item><term>Nutrition</term></item><item><term>Intestinal Bacteria</term></item></list></keywords></textClass></profileDesc><revisionDesc><change when="2012-11-07">Created</change><change 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abbrev-type="publisher">Gut</abbrev-journal-title><abbrev-journal-title>Gut</abbrev-journal-title><issn pub-type="ppub">0017-5749</issn><issn pub-type="epub">1468-3288</issn><publisher><publisher-name>BMJ Publishing Group Ltd and British Society of Gastroenterology</publisher-name></publisher></journal-meta><article-meta><article-id pub-id-type="publisher-id">gutjnl-2012-303304</article-id><article-id pub-id-type="doi">10.1136/gutjnl-2012-303304</article-id><article-id pub-id-type="other">gutjnl;62/8/1112</article-id><article-id pub-id-type="other">gutjnl;gutjnl-2012-303304</article-id><article-id pub-id-type="pmid">23135760</article-id><article-id pub-id-type="other">1112</article-id><article-id pub-id-type="other">gutjnl-2012-303304</article-id><article-categories><subj-group subj-group-type="heading"><subject>Nutrition</subject></subj-group><subj-group subj-group-type="hwp-journal-coll"><subject>Open access</subject></subj-group></article-categories><title-group><article-title>Insight into the prebiotic concept: lessons from an exploratory, double blind intervention study with inulin-type fructans in obese women</article-title></title-group><contrib-group><contrib contrib-type="author"><name><surname>Dewulf</surname><given-names>Evelyne M</given-names></name><xref ref-type="aff" rid="af1">1</xref></contrib><contrib contrib-type="author"><name><surname>Cani</surname><given-names>Patrice D</given-names></name><xref ref-type="aff" rid="af1">1</xref></contrib><contrib contrib-type="author"><name><surname>Claus</surname><given-names>Sandrine P</given-names></name><xref ref-type="aff" rid="af2">2</xref></contrib><contrib contrib-type="author"><name><surname>Fuentes</surname><given-names>Susana</given-names></name><xref ref-type="aff" rid="af3">3</xref></contrib><contrib contrib-type="author"><name><surname>Puylaert</surname><given-names>Philippe GB</given-names></name><xref ref-type="aff" rid="af3">3</xref></contrib><contrib contrib-type="author"><name><surname>Neyrinck</surname><given-names>Audrey M</given-names></name><xref ref-type="aff" rid="af1">1</xref></contrib><contrib contrib-type="author"><name><surname>Bindels</surname><given-names>Laure B</given-names></name><xref ref-type="aff" rid="af1">1</xref></contrib><contrib contrib-type="author"><name><surname>de Vos</surname><given-names>Willem M</given-names></name><xref ref-type="aff" rid="af3">3</xref></contrib><contrib contrib-type="author"><name><surname>Gibson</surname><given-names>Glenn R</given-names></name><xref ref-type="aff" rid="af2">2</xref></contrib><contrib contrib-type="author"><name><surname>Thissen</surname><given-names>Jean-Paul</given-names></name><xref ref-type="aff" rid="af4">4</xref></contrib><contrib contrib-type="author"><name><surname>Delzenne</surname><given-names>Nathalie M</given-names></name><xref ref-type="aff" rid="af1">1</xref></contrib></contrib-group><aff id="af1"><label>1</label><addr-line>Metabolism and Nutrition Research Group</addr-line>, <institution>LDRI, Université catholique de Louvain</institution>, <addr-line>Bruxelles</addr-line>, <country>Belgium</country></aff><aff id="af2"><label>2</label><addr-line>Department of Food and Nutritional Sciences</addr-line>, <institution>University of Reading</institution>, <addr-line>Reading</addr-line>, <country>UK</country></aff><aff id="af3"><label>3</label><addr-line>Laboratory of Microbiology</addr-line>, <institution>Wageningen University and Research Centre</institution>, <addr-line>Wageningen</addr-line>, <country>The Netherlands</country></aff><aff id="af4"><label>4</label><addr-line>Pole of Endocrinology, Diabetology and Nutrition</addr-line>, <institution>IREC, Université catholique de Louvain</institution>, <addr-line>Bruxelles</addr-line>, <country>Belgium</country></aff><author-notes><corresp><label>Correspondence to</label> Professor Nathalie M Delzenne, Metabolism and Nutrition Research Group, Louvain Drug Research Institute, Université catholique de Louvain, Avenue E. Mounier 73,PO Box B1.73.11, 1200 Brussels, Belgium; <email>nathalie.delzenne@uclouvain.be</email></corresp><fn><p>PDC and SPC contributed equally</p></fn></author-notes><pub-date pub-type="ppub"><month>8</month><year>2013</year></pub-date><pub-date pub-type="epub-original"><day>7</day><month>11</month><year>2012</year></pub-date><pub-date pub-type="epub"><day>7</day><month>11</month><year>2012</year></pub-date><volume>62</volume><volume-id pub-id-type="other">62</volume-id><volume-id pub-id-type="other">62</volume-id><issue>8</issue><issue-id pub-id-type="other">gutjnl;62/8</issue-id><issue-id pub-id-type="other">8</issue-id><issue-id pub-id-type="other">62/8</issue-id><fpage>1112</fpage><history><date date-type="received"><day>9</day><month>10</month><year>2012</year></date><date date-type="accepted"><day>9</day><month>10</month><year>2012</year></date></history><permissions><copyright-statement>Published by the BMJ Publishing Group Limited. For permission to use (where not already granted under a licence) please go to http://group.bmj.com/group/rights-licensing/permissions</copyright-statement><copyright-year>2013</copyright-year><license license-type="open-access" xlink:href="http://creativecommons.org/licenses/"><p>This is an Open Access article distributed in accordance with the Creative Commons Attribution Non Commercial (CC BY-NC 3.0) license, which permits others to distribute, remix, adapt, build upon this work non-commercially, and license their derivative works on different terms, provided the original work is properly cited and the use is non-commercial. See: <ext-link ext-link-type="uri" xlink:href="http://creativecommons.org/licenses/by-nc/3.0/">http://creativecommons.org/licenses/by-nc/3.0/</ext-link></p></license></permissions><self-uri content-type="pdf" xlink:role="full-text" xlink:href="gutjnl-62-1112.pdf"></self-uri><related-article id="RA1" related-article-type="companion" ext-link-type="doi" xlink:href="10.1136/gutjnl-2012-303908"></related-article><related-article id="RA2" related-article-type="companion" ext-link-type="doi" xlink:href="10.1136/gutjnl-2012-304393"></related-article><abstract><sec><title>Objective</title><p>To highlight the contribution of the gut microbiota to the modulation of host metabolism by dietary inulin-type fructans (ITF prebiotics) in obese women.</p></sec><sec><title>Methods</title><p>A double blind, placebo controlled, intervention study was performed with 30 obese women treated with ITF prebiotics (inulin/oligofructose 50/50 mix; n=15) or placebo (maltodextrin; n=15) for 3 months (16 g/day). Blood, faeces and urine sampling, oral glucose tolerance test, homeostasis model assessment and impedancemetry were performed before and after treatment. The gut microbial composition in faeces was analysed by phylogenetic microarray and qPCR analysis of 16S rDNA. Plasma and urine metabolic profiles were analysed by <sup>1</sup>H-NMR spectroscopy.</p></sec><sec><title>Results</title><p>Treatment with ITF prebiotics, but not the placebo, led to an increase in <italic>Bifidobacterium</italic> and <italic>Faecalibacterium prausnitzii</italic>; both bacteria negatively correlated with serum lipopolysaccharide levels. ITF prebiotics also decreased <italic>Bacteroides intestinalis</italic>, <italic>Bacteroides vulgatus</italic> and <italic>Propionibacterium</italic>, an effect associated with a slight decrease in fat mass and with plasma lactate and phosphatidylcholine levels. No clear treatment clustering could be detected for gut microbial analysis or plasma and urine metabolomic profile analyses. However, ITF prebiotics led to subtle changes in the gut microbiota that may importantly impact on several key metabolites implicated in obesity and/or diabetes.</p></sec><sec><title>Conclusions</title><p>ITF prebiotics selectively changed the gut microbiota composition in obese women, leading to modest changes in host metabolism, as suggested by the correlation between some bacterial species and metabolic endotoxaemia or metabolomic signatures.</p></sec></abstract><kwd-group><kwd>Obesity</kwd><kwd>Prebiotic</kwd><kwd>Nutrition</kwd><kwd>Intestinal Bacteria</kwd></kwd-group></article-meta></front></article></istex:document></istex:metadataXml><mods version="3.6"><titleInfo><title>Insight into the prebiotic concept: lessons from an exploratory, double blind intervention study with inulin-type fructans in obese women</title></titleInfo><titleInfo type="alternative" contentType="CDATA"><title>Insight into the prebiotic concept: lessons from an exploratory, double blind intervention study with inulin-type fructans in obese women</title></titleInfo><name type="personal"><namePart type="given">Evelyne M</namePart><namePart type="family">Dewulf</namePart><affiliation>Metabolism and Nutrition Research Group, LDRI, Université catholique de Louvain, Bruxelles, Belgium</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">Patrice D</namePart><namePart type="family">Cani</namePart><affiliation>Metabolism and Nutrition Research Group, LDRI, Université catholique de Louvain, Bruxelles, Belgium</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">Sandrine P</namePart><namePart type="family">Claus</namePart><affiliation>Department of Food and Nutritional Sciences, University of Reading, Reading, UK</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">Susana</namePart><namePart type="family">Fuentes</namePart><affiliation>Laboratory of Microbiology, Wageningen University and Research Centre, Wageningen, The Netherlands</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">Philippe GB</namePart><namePart type="family">Puylaert</namePart><affiliation>Laboratory of Microbiology, Wageningen University and Research Centre, Wageningen, The Netherlands</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">Audrey M</namePart><namePart type="family">Neyrinck</namePart><affiliation>Metabolism and Nutrition Research Group, LDRI, Université catholique de Louvain, Bruxelles, Belgium</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">Laure B</namePart><namePart type="family">Bindels</namePart><affiliation>Metabolism and Nutrition Research Group, LDRI, Université catholique de Louvain, Bruxelles, Belgium</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">Willem M</namePart><namePart type="family">de Vos</namePart><affiliation>Laboratory of Microbiology, Wageningen University and Research Centre, Wageningen, The Netherlands</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">Glenn R</namePart><namePart type="family">Gibson</namePart><affiliation>Department of Food and Nutritional Sciences, University of Reading, Reading, UK</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">Jean-Paul</namePart><namePart type="family">Thissen</namePart><affiliation>Pole of Endocrinology, Diabetology and Nutrition, IREC, Université catholique de Louvain, Bruxelles, Belgium</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">Nathalie M</namePart><namePart type="family">Delzenne</namePart><affiliation>Metabolism and Nutrition Research Group, LDRI, Université catholique de Louvain, Bruxelles, Belgium</affiliation><role><roleTerm type="text">author</roleTerm></role></name><typeOfResource>text</typeOfResource><genre type="research-article" displayLabel="research-article"></genre><subject><genre>hwp-journal-coll</genre><topic>Open access</topic></subject><originInfo><publisher>BMJ Publishing Group Ltd and British Society of Gastroenterology</publisher><dateIssued encoding="w3cdtf">2013-08</dateIssued><dateCreated encoding="w3cdtf">2012-11-07</dateCreated><copyrightDate encoding="w3cdtf">2013</copyrightDate></originInfo><language><languageTerm type="code" authority="iso639-2b">eng</languageTerm><languageTerm type="code" authority="rfc3066">en</languageTerm></language><physicalDescription><internetMediaType>text/html</internetMediaType></physicalDescription><abstract>Objective To highlight the contribution of the gut microbiota to the modulation of host metabolism by dietary inulin-type fructans (ITF prebiotics) in obese women. Methods A double blind, placebo controlled, intervention study was performed with 30 obese women treated with ITF prebiotics (inulin/oligofructose 50/50 mix; n=15) or placebo (maltodextrin; n=15) for 3 months (16 g/day). Blood, faeces and urine sampling, oral glucose tolerance test, homeostasis model assessment and impedancemetry were performed before and after treatment. The gut microbial composition in faeces was analysed by phylogenetic microarray and qPCR analysis of 16S rDNA. Plasma and urine metabolic profiles were analysed by 1H-NMR spectroscopy. Results Treatment with ITF prebiotics, but not the placebo, led to an increase in Bifidobacterium and Faecalibacterium prausnitzii; both bacteria negatively correlated with serum lipopolysaccharide levels. ITF prebiotics also decreased Bacteroides intestinalis, Bacteroides vulgatus and Propionibacterium, an effect associated with a slight decrease in fat mass and with plasma lactate and phosphatidylcholine levels. No clear treatment clustering could be detected for gut microbial analysis or plasma and urine metabolomic profile analyses. However, ITF prebiotics led to subtle changes in the gut microbiota that may importantly impact on several key metabolites implicated in obesity and/or diabetes. Conclusions ITF prebiotics selectively changed the gut microbiota composition in obese women, leading to modest changes in host metabolism, as suggested by the correlation between some bacterial species and metabolic endotoxaemia or metabolomic signatures.</abstract><note type="footnotes">PDC and SPC contributed equally</note><subject><genre>keywords</genre><topic>Obesity</topic><topic>Prebiotic</topic><topic>Nutrition</topic><topic>Intestinal Bacteria</topic></subject><relatedItem type="host"><titleInfo><title>Gut</title></titleInfo><titleInfo type="abbreviated"><title>Gut</title></titleInfo><genre type="journal">journal</genre><identifier type="ISSN">0017-5749</identifier><identifier type="eISSN">1468-3288</identifier><identifier type="PublisherID">gut</identifier><identifier type="PublisherID-hwp">gutjnl</identifier><identifier type="PublisherID-nlm-ta">Gut</identifier><part><date>2013</date><detail type="volume"><caption>vol.</caption><number>62</number></detail><detail type="issue"><caption>no.</caption><number>8</number></detail><extent unit="pages"><start>1112</start></extent></part></relatedItem><relatedItem type="reviewOf"><identifier type="doi">10.1136/gutjnl-2012-303908</identifier></relatedItem><relatedItem type="reviewOf"><identifier type="doi">10.1136/gutjnl-2012-304393</identifier></relatedItem><identifier type="istex">E750762DD9B4B139E4D5AB53F3CCB6D48AB1D2CE</identifier><identifier type="DOI">10.1136/gutjnl-2012-303304</identifier><identifier type="href">gutjnl-62-1112.pdf</identifier><identifier type="ArticleID">gutjnl-2012-303304</identifier><identifier type="PMID">23135760</identifier><identifier type="Related-article-href">10.1136/gutjnl-2012-303908</identifier><identifier type="Related-article-href">10.1136/gutjnl-2012-304393</identifier><identifier type="related-article-ID">RA1</identifier><identifier type="local">gutjnl;62/8/1112</identifier><accessCondition type="use and reproduction" contentType="open-access">This is an Open Access article distributed in accordance with the Creative Commons Attribution Non Commercial (CC BY-NC 3.0) license, which permits others to distribute, remix, adapt, build upon this work non-commercially, and license their derivative works on different terms, provided the original work is properly cited and the use is non-commercial. 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