SNPbox: web-based high-throughput primer design from gene to genome
Identifieur interne : 000502 ( Istex/Corpus ); précédent : 000501; suivant : 000503SNPbox: web-based high-throughput primer design from gene to genome
Auteurs : Stefan Weckx ; Peter De Rijk ; Christine Van Broeckhoven ; Jurgen Del-FaveroSource :
- Nucleic Acids Research [ 0305-1048 ] ; 2004-07-01.
Abstract
SNPbox is a modular software package that automates the design of PCR primers for large-scale amplification and sequencing projects in a standardized manner resulting in high-quality PCR amplicons with a low failure rate. Here, we present the SNPbox web server at http://www.SNPbox.org, which hosts the SNPbox web service as well as the data from SNPbox analysis of all Ensembl exons. The data of this genome-wide SNPbox application can be visualized in Ensembl's ContigView through a DAS (distributed annotation system) annotation server.
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DOI: 10.1093/nar/gkh369
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Acids Res.</abbrev-journal-title><issn pub-type="ppub">0305-1048</issn><issn pub-type="epub">1362-4962</issn><publisher><publisher-name>Oxford University Press</publisher-name></publisher></journal-meta><article-meta><article-id pub-id-type="other">gkh369</article-id><article-id pub-id-type="doi">10.1093/nar/gkh369</article-id><article-categories><subj-group subj-group-type="heading"><subject>Articles</subject></subj-group></article-categories><title-group><article-title>SNPbox: web-based high-throughput primer design from gene to genome</article-title></title-group><contrib-group><contrib contrib-type="author"><name><surname>Weckx</surname><given-names>Stefan</given-names></name></contrib><contrib contrib-type="author"><name><surname>De Rijk</surname><given-names>Peter</given-names></name></contrib><contrib contrib-type="author"><name><surname>Van Broeckhoven</surname><given-names>Christine</given-names></name></contrib><contrib contrib-type="author"><name><surname>Del-Favero</surname><given-names>Jurgen</given-names></name><xref rid="COR1">*</xref></contrib><aff>Department of Molecular Genetics, Bioinformatics Unit, Flanders Interuniversity Institute for Biotechnology, University of Antwerp, Belgium</aff></contrib-group><author-notes><corresp id="COR1"><label>*</label>To whom correspondence should be addressed. Tel: +32 3 820 2321; Fax: +32 3 820 2541; Email: <ext-link xlink:href="jurgen.delfavero@ua.ac.be" ext-link-type="email">jurgen.delfavero@ua.ac.be</ext-link></corresp></author-notes><pub-date pub-type="ppub"><day>1</day><month>7</month><year>2004</year></pub-date><volume>32</volume><issue>suppl_2</issue><fpage>W170</fpage><lpage>W172</lpage><permissions><copyright-statement>© 2004, the authors
<italic>Nucleic Acids Research, Vol. 32, Web Server issue © Oxford University Press 2004; all rights reserved</italic></copyright-statement><copyright-year>2004</copyright-year><license license-type="open-access"><p></p></license></permissions><abstract xml:lang="en"><p>SNPbox is a modular software package that automates the design of PCR primers for large-scale amplification and sequencing projects in a standardized manner resulting in high-quality PCR amplicons with a low failure rate. Here, we present the SNPbox web server at <ext-link xlink:href="http://www.SNPbox.org" ext-link-type="url">http://www.SNPbox.org</ext-link>, which hosts the SNPbox web service as well as the data from SNPbox analysis of all Ensembl exons. The data of this genome-wide SNPbox application can be visualized in Ensembl's <italic>ContigView</italic> through a DAS (distributed annotation system) annotation server.</p></abstract><custom-meta-wrap><custom-meta><meta-name>hwp-legacy-fpage</meta-name><meta-value>W170</meta-value></custom-meta><custom-meta><meta-name>hwp-legacy-dochead</meta-name><meta-value>Article</meta-value></custom-meta></custom-meta-wrap></article-meta><notes><p content-type="arthw-misc">Received February 12, 2004; Revised and Accepted March 9, 2004
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