Pharmaceutical analysis by supercritical fluid chromatography: Optimization of the mobile phase composition on a 2‐ethylpyridine column
Identifieur interne : 000171 ( Istex/Corpus ); précédent : 000170; suivant : 000172Pharmaceutical analysis by supercritical fluid chromatography: Optimization of the mobile phase composition on a 2‐ethylpyridine column
Auteurs : Claudio Brunelli ; Yining Zhao ; Melissa-Hanna Brown ; Pat SandraSource :
- Journal of Separation Science [ 1615-9306 ] ; 2008-05.
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Abstract
The separation of neutral, acidic, and basic pharmaceuticals with diverse physicochemical properties by packed column supercritical fluid chromatography (pSFC) on a 2‐ethylpyridine column (25 cm×4.6 mm id, 3 μm particles) is presented. The optimization strategy involved separations at 100% methanol (MeOH) and at 50% MeOH/50% ACN while keeping the peak symmetry additives formic acid (FA) and isopropylamine (IPA) at constant levels of 0.25% v/v. By plotting the adjusted retention times as a function of the MeOH/ACN ratio, an optimal modifier ratio composition of 65% MeOH/35% ACN was found. The total set of 26 neutral, acidic, and basic pharmaceuticals was analyzed and the optimal composition experimentally verified. This mobile phase composition is currently used in pharmaceutical method development and open‐access generic screening environments.
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DOI: 10.1002/jssc.200700555
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ISTEX:FF742E545C9AC68FAE375B66A5A74E68847D5114Le document en format XML
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The optimization strategy involved separations at 100% methanol (MeOH) and at 50% MeOH/50% ACN while keeping the peak symmetry additives formic acid (FA) and isopropylamine (IPA) at constant levels of 0.25% v/v. By plotting the adjusted retention times as a function of the MeOH/ACN ratio, an optimal modifier ratio composition of 65% MeOH/35% ACN was found. The total set of 26 neutral, acidic, and basic pharmaceuticals was analyzed and the optimal composition experimentally verified. This mobile phase composition is currently used in pharmaceutical method development and open‐access generic screening environments.</div></front></TEI><istex><corpusName>wiley</corpusName><author><json:item><name>Claudio Brunelli</name><affiliations><json:string>Pfizer Analytical Research Centre, Ghent University, Ghent, Belgium. 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The optimization strategy involved separations at 100% methanol (MeOH) and at 50% MeOH/50% ACN while keeping the peak symmetry additives formic acid (FA) and isopropylamine (IPA) at constant levels of 0.25% v/v. By plotting the adjusted retention times as a function of the MeOH/ACN ratio, an optimal modifier ratio composition of 65% MeOH/35% ACN was found. The total set of 26 neutral, acidic, and basic pharmaceuticals was analyzed and the optimal composition experimentally verified. 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The optimization strategy involved separations at 100% methanol (MeOH) and at 50% MeOH/50% ACN while keeping the peak symmetry additives formic acid (FA) and isopropylamine (IPA) at constant levels of 0.25% v/v. By plotting the adjusted retention times as a function of the MeOH/ACN ratio, an optimal modifier ratio composition of 65% MeOH/35% ACN was found. The total set of 26 neutral, acidic, and basic pharmaceuticals was analyzed and the optimal composition experimentally verified. This mobile phase composition is currently used in pharmaceutical method development and open‐access generic screening environments.</abstract><subject lang="en"><genre>keywords</genre><topic>2‐Ethylpyridine stationary phase</topic><topic>Mobile phase optimization</topic><topic>Pharmaceuticals</topic><topic>Supercritical fluid chromatography</topic></subject><relatedItem type="host"><titleInfo><title>Journal of Separation Science</title></titleInfo><titleInfo type="abbreviated"><title>J. Sep. 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