Serveur d'exploration sur l'OCR

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The optical cell rotator.

Identifieur interne : 000044 ( PubMed/Checkpoint ); précédent : 000043; suivant : 000045

The optical cell rotator.

Auteurs : Moritz K. Kreysing [Allemagne] ; Tobias Kiessling ; Anatol Fritsch ; Christian Dietrich ; Jochen R. Guck ; Josef A. K S

Source :

RBID : pubmed:18852807

English descriptors

Abstract

The optical cell rotator (OCR) is a modified dual-beam laser trap for the holding and controlled rotation of suspended dielectric microparticles, such as cells. In contrast to optical tweezers, OCR uses two counter-propagating divergent laser beams, which are shaped and delivered by optical fibers. The rotation of a trapped specimen is carried out by the rotation of a dual-mode fiber, emitting an asymmetric laser beam. Experiments were performed on human erythrocytes, promyelocytic leukemia cells (HL60), and cell clusters (MCF-7). Since OCR permits the rotation of cells around an axis perpendicular to the optical axis of any microscope and is fully decoupled from imaging optics, it could be a suitable and expedient tool for tomographic microscopy.

PubMed: 18852807


Affiliations:


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pubmed:18852807

Le document en format XML

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<nlm:affiliation>Department for Experimental Physics I, University of Leipzig, Leipzig, Germany. mkk26@cam.ac.uk</nlm:affiliation>
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<div type="abstract" xml:lang="en">The optical cell rotator (OCR) is a modified dual-beam laser trap for the holding and controlled rotation of suspended dielectric microparticles, such as cells. In contrast to optical tweezers, OCR uses two counter-propagating divergent laser beams, which are shaped and delivered by optical fibers. The rotation of a trapped specimen is carried out by the rotation of a dual-mode fiber, emitting an asymmetric laser beam. Experiments were performed on human erythrocytes, promyelocytic leukemia cells (HL60), and cell clusters (MCF-7). Since OCR permits the rotation of cells around an axis perpendicular to the optical axis of any microscope and is fully decoupled from imaging optics, it could be a suitable and expedient tool for tomographic microscopy.</div>
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