Influence of phage T3 and T7 gene functions on a type III(EcoP1) DNA restriction-modification system in vivo.
Identifieur interne : 004249 ( Main/Merge ); précédent : 004248; suivant : 004250Influence of phage T3 and T7 gene functions on a type III(EcoP1) DNA restriction-modification system in vivo.
Auteurs : D H Krüger ; M. Reuter ; S. Hansen ; C. SchroederSource :
- Molecular & general genetics : MGG [ 0026-8925 ] ; 1982.
English descriptors
- KwdEn :
- MESH :
- chemical , genetics : DNA, Bacterial, DNA, Viral.
- chemical : DNA Restriction Enzymes.
- genetics : Escherichia coli, T-Phages.
- DNA Replication, Genes, Viral, Kinetics, Mutation, Transformation, Bacterial, Virus Replication.
Abstract
The ocr+ gene function (gp 0.3) of bacteriophages T3 and T7 not only counteracts type I (EcoB, EcoK) but also type III restriction endonucleases (EcoP1). Despite the presence of recognition sites, phage DNA as well as simultaneously introduced plasmid DNA are protected by ocr+ expression against both the endonucleolytic and the methylating activities of the EcoP1 enzyme. Nevertheless, the EcoP1 protein causes the exclusion of T3 and T7 in P1-lysogenic cells, apparently by exerting a repressor-like effect on phage gene expression. T3 which induces an S-adenosylmethionine hydrolase is less susceptible to the repressor effect of the SAM-stimulated EcoP1 enzyme. The abundance of EcoP1 recognition sites in the T7 genome is explained by their near identity with the T7 DNA primase recognition site.
PubMed: 6285143
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pubmed:6285143Le document en format XML
<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">Influence of phage T3 and T7 gene functions on a type III(EcoP1) DNA restriction-modification system in vivo.</title><author><name sortKey="Kruger, D H" sort="Kruger, D H" uniqKey="Kruger D" first="D H" last="Krüger">D H Krüger</name></author><author><name sortKey="Reuter, M" sort="Reuter, M" uniqKey="Reuter M" first="M" last="Reuter">M. Reuter</name></author><author><name sortKey="Hansen, S" sort="Hansen, S" uniqKey="Hansen S" first="S" last="Hansen">S. Hansen</name></author><author><name sortKey="Schroeder, C" sort="Schroeder, C" uniqKey="Schroeder C" first="C" last="Schroeder">C. Schroeder</name></author></titleStmt><publicationStmt><idno type="wicri:source">PubMed</idno><date when="1982">1982</date><idno type="RBID">pubmed:6285143</idno><idno type="pmid">6285143</idno><idno type="wicri:Area/PubMed/Corpus">000094</idno><idno type="wicri:Area/PubMed/Curation">000094</idno><idno type="wicri:Area/PubMed/Checkpoint">000094</idno><idno type="wicri:Area/Ncbi/Merge">000269</idno><idno type="wicri:Area/Ncbi/Curation">000269</idno><idno type="wicri:Area/Ncbi/Checkpoint">000269</idno><idno type="wicri:doubleKey">0026-8925:1982:Kruger D:influence:of:phage</idno><idno type="wicri:Area/Main/Merge">004249</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en">Influence of phage T3 and T7 gene functions on a type III(EcoP1) DNA restriction-modification system in vivo.</title><author><name sortKey="Kruger, D H" sort="Kruger, D H" uniqKey="Kruger D" first="D H" last="Krüger">D H Krüger</name></author><author><name sortKey="Reuter, M" sort="Reuter, M" uniqKey="Reuter M" first="M" last="Reuter">M. Reuter</name></author><author><name sortKey="Hansen, S" sort="Hansen, S" uniqKey="Hansen S" first="S" last="Hansen">S. Hansen</name></author><author><name sortKey="Schroeder, C" sort="Schroeder, C" uniqKey="Schroeder C" first="C" last="Schroeder">C. Schroeder</name></author></analytic><series><title level="j">Molecular & general genetics : MGG</title><idno type="ISSN">0026-8925</idno><imprint><date when="1982" type="published">1982</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>DNA Replication</term><term>DNA Restriction Enzymes</term><term>DNA, Bacterial (genetics)</term><term>DNA, Viral (genetics)</term><term>Escherichia coli (genetics)</term><term>Genes, Viral</term><term>Kinetics</term><term>Mutation</term><term>T-Phages (genetics)</term><term>Transformation, Bacterial</term><term>Virus Replication</term></keywords><keywords scheme="MESH" type="chemical" qualifier="genetics" xml:lang="en"><term>DNA, Bacterial</term><term>DNA, Viral</term></keywords><keywords scheme="MESH" type="chemical" xml:lang="en"><term>DNA Restriction Enzymes</term></keywords><keywords scheme="MESH" qualifier="genetics" xml:lang="en"><term>Escherichia coli</term><term>T-Phages</term></keywords><keywords scheme="MESH" xml:lang="en"><term>DNA Replication</term><term>Genes, Viral</term><term>Kinetics</term><term>Mutation</term><term>Transformation, Bacterial</term><term>Virus Replication</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">The ocr+ gene function (gp 0.3) of bacteriophages T3 and T7 not only counteracts type I (EcoB, EcoK) but also type III restriction endonucleases (EcoP1). Despite the presence of recognition sites, phage DNA as well as simultaneously introduced plasmid DNA are protected by ocr+ expression against both the endonucleolytic and the methylating activities of the EcoP1 enzyme. Nevertheless, the EcoP1 protein causes the exclusion of T3 and T7 in P1-lysogenic cells, apparently by exerting a repressor-like effect on phage gene expression. T3 which induces an S-adenosylmethionine hydrolase is less susceptible to the repressor effect of the SAM-stimulated EcoP1 enzyme. The abundance of EcoP1 recognition sites in the T7 genome is explained by their near identity with the T7 DNA primase recognition site.</div></front></TEI></record>Pour manipuler ce document sous Unix (Dilib)
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