Relation between intracellular Ca2+ signals and Ca2+-activated Cl− current in Xenopus oocytes
Identifieur interne : 002C63 ( Main/Exploration ); précédent : 002C62; suivant : 002C64Relation between intracellular Ca2+ signals and Ca2+-activated Cl− current in Xenopus oocytes
Auteurs : I. Parker [États-Unis] ; Y. Yao [États-Unis]Source :
- Cell Calcium [ 0143-4160 ] ; 1993.
Abstract
Activation of inositol 1,4,5-trisphosphate (InsP3) signalling in Xenopus oocytes causes intracellular Ca2+ mobilization and thereby activates a Ca2+-dependent Cl− membrane conductance. Measurements of cytosolic Ca2+ levels using fluorescent indicators, however, revealed little correspondence with Cl− currents. Intracellular photorelease of InsP3 from a caged precursor evoked transient currents that peaked while the Ca2+-fluorescence signal was rising, and subsequently declined within a few seconds, even though the Ca2+ signal remained elevated much longer. Also, Cl− currents evoked by agonist activation showed transient spikes while a wave of Ca2+ liberation swept across the cell, but then decreased when the Ca2+ signal attained a maximal level. Thus, the Cl− current corresponded better to the rate of rise of intracellular free Ca2+, rather than to its steady state level. Experiments using paired flashes to photolyse caged InsP3 and caged Ca2+ indicated that this relationship did not arise through desensitization or inactivation of the Cl− conductance. Furthermore, fluorescence measurements made at different depths into the cell using a confocal microscope revealed no evidence that a rapid decline of local Ca2+ levels near the plasma membrane was responsible for the decay of Ca2+-activated Cl− current. Instead, Cl− channels may show an adaptive or incremental response to Ca2+, which is likely to be important for the encoding and transmission of information by Ca2+ spikes.
Url:
DOI: 10.1016/0143-4160(94)90067-1
Affiliations:
Links toward previous steps (curation, corpus...)
- to stream Istex, to step Corpus: 000D89
- to stream Istex, to step Curation: 000D57
- to stream Istex, to step Checkpoint: 001F58
- to stream Main, to step Merge: 002E30
- to stream Main, to step Curation: 002C63
Le document en format XML
<record><TEI wicri:istexFullTextTei="biblStruct"><teiHeader><fileDesc><titleStmt><title>Relation between intracellular Ca2+ signals and Ca2+-activated Cl− current in Xenopus oocytes</title>
<author><name sortKey="Parker, I" sort="Parker, I" uniqKey="Parker I" first="I." last="Parker">I. Parker</name>
</author>
<author><name sortKey="Yao, Y" sort="Yao, Y" uniqKey="Yao Y" first="Y." last="Yao">Y. Yao</name>
</author>
</titleStmt>
<publicationStmt><idno type="wicri:source">ISTEX</idno>
<idno type="RBID">ISTEX:0D331AF8321E2728FB775DC21643C4B3164E485C</idno>
<date when="1994" year="1994">1994</date>
<idno type="doi">10.1016/0143-4160(94)90067-1</idno>
<idno type="url">https://api.istex.fr/document/0D331AF8321E2728FB775DC21643C4B3164E485C/fulltext/pdf</idno>
<idno type="wicri:Area/Istex/Corpus">000D89</idno>
<idno type="wicri:Area/Istex/Curation">000D57</idno>
<idno type="wicri:Area/Istex/Checkpoint">001F58</idno>
<idno type="wicri:doubleKey">0143-4160:1994:Parker I:relation:between:intracellular</idno>
<idno type="wicri:Area/Main/Merge">002E30</idno>
<idno type="wicri:Area/Main/Curation">002C63</idno>
<idno type="wicri:Area/Main/Exploration">002C63</idno>
</publicationStmt>
<sourceDesc><biblStruct><analytic><title level="a">Relation between intracellular Ca2+ signals and Ca2+-activated Cl− current in Xenopus oocytes</title>
<author><name sortKey="Parker, I" sort="Parker, I" uniqKey="Parker I" first="I." last="Parker">I. Parker</name>
<affiliation wicri:level="2"><country xml:lang="fr">États-Unis</country>
<wicri:regionArea>Laboratory of Cellular and Molecular Neurobiology, Department of Psychobiology, University of Califormia at Irvine, Irvine, California</wicri:regionArea>
<placeName><region type="state">Californie</region>
</placeName>
</affiliation>
</author>
<author><name sortKey="Yao, Y" sort="Yao, Y" uniqKey="Yao Y" first="Y." last="Yao">Y. Yao</name>
<affiliation wicri:level="2"><country xml:lang="fr">États-Unis</country>
<wicri:regionArea>Laboratory of Cellular and Molecular Neurobiology, Department of Psychobiology, University of Califormia at Irvine, Irvine, California</wicri:regionArea>
<placeName><region type="state">Californie</region>
</placeName>
</affiliation>
</author>
</analytic>
<monogr></monogr>
<series><title level="j">Cell Calcium</title>
<title level="j" type="abbrev">YCECA</title>
<idno type="ISSN">0143-4160</idno>
<imprint><publisher>ELSEVIER</publisher>
<date type="published" when="1993">1993</date>
<biblScope unit="volume">15</biblScope>
<biblScope unit="issue">4</biblScope>
<biblScope unit="page" from="276">276</biblScope>
<biblScope unit="page" to="288">288</biblScope>
</imprint>
<idno type="ISSN">0143-4160</idno>
</series>
<idno type="istex">0D331AF8321E2728FB775DC21643C4B3164E485C</idno>
<idno type="DOI">10.1016/0143-4160(94)90067-1</idno>
<idno type="PII">0143-4160(94)90067-1</idno>
</biblStruct>
</sourceDesc>
<seriesStmt><idno type="ISSN">0143-4160</idno>
</seriesStmt>
</fileDesc>
<profileDesc><textClass></textClass>
<langUsage><language ident="en">en</language>
</langUsage>
</profileDesc>
</teiHeader>
<front><div type="abstract" xml:lang="en">Activation of inositol 1,4,5-trisphosphate (InsP3) signalling in Xenopus oocytes causes intracellular Ca2+ mobilization and thereby activates a Ca2+-dependent Cl− membrane conductance. Measurements of cytosolic Ca2+ levels using fluorescent indicators, however, revealed little correspondence with Cl− currents. Intracellular photorelease of InsP3 from a caged precursor evoked transient currents that peaked while the Ca2+-fluorescence signal was rising, and subsequently declined within a few seconds, even though the Ca2+ signal remained elevated much longer. Also, Cl− currents evoked by agonist activation showed transient spikes while a wave of Ca2+ liberation swept across the cell, but then decreased when the Ca2+ signal attained a maximal level. Thus, the Cl− current corresponded better to the rate of rise of intracellular free Ca2+, rather than to its steady state level. Experiments using paired flashes to photolyse caged InsP3 and caged Ca2+ indicated that this relationship did not arise through desensitization or inactivation of the Cl− conductance. Furthermore, fluorescence measurements made at different depths into the cell using a confocal microscope revealed no evidence that a rapid decline of local Ca2+ levels near the plasma membrane was responsible for the decay of Ca2+-activated Cl− current. Instead, Cl− channels may show an adaptive or incremental response to Ca2+, which is likely to be important for the encoding and transmission of information by Ca2+ spikes.</div>
</front>
</TEI>
<affiliations><list><country><li>États-Unis</li>
</country>
<region><li>Californie</li>
</region>
</list>
<tree><country name="États-Unis"><region name="Californie"><name sortKey="Parker, I" sort="Parker, I" uniqKey="Parker I" first="I." last="Parker">I. Parker</name>
</region>
<name sortKey="Yao, Y" sort="Yao, Y" uniqKey="Yao Y" first="Y." last="Yao">Y. Yao</name>
</country>
</tree>
</affiliations>
</record>
Pour manipuler ce document sous Unix (Dilib)
EXPLOR_STEP=$WICRI_ROOT/Ticri/CIDE/explor/OcrV1/Data/Main/Exploration
HfdSelect -h $EXPLOR_STEP/biblio.hfd -nk 002C63 | SxmlIndent | more
Ou
HfdSelect -h $EXPLOR_AREA/Data/Main/Exploration/biblio.hfd -nk 002C63 | SxmlIndent | more
Pour mettre un lien sur cette page dans le réseau Wicri
{{Explor lien |wiki= Ticri/CIDE |area= OcrV1 |flux= Main |étape= Exploration |type= RBID |clé= ISTEX:0D331AF8321E2728FB775DC21643C4B3164E485C |texte= Relation between intracellular Ca2+ signals and Ca2+-activated Cl− current in Xenopus oocytes }}
![]() | This area was generated with Dilib version V0.6.32. | ![]() |