Manipulation of individual viruses: friction and mechanical properties.
Identifieur interne : 000983 ( Pmc/Curation ); précédent : 000982; suivant : 000984Manipulation of individual viruses: friction and mechanical properties.
Auteurs : M R Falvo ; S. Washburn ; R. Superfine ; M. Finch ; F P Brooks ; V. Chi ; R M TaylorSource :
- Biophysical Journal [ 0006-3495 ] ; 1997.
Abstract
We present our results on the manipulation of individual viruses using an advanced interface for atomic force microscopes (AFMs). We show that the viruses can be dissected, rotated, and translated with great facility. We interpret the behavior of tobacco mosaic virus with a mechanical model that makes explicit the competition between sample-substrate lateral friction and the flexural rigidity of the manipulated object. The manipulation behavior of tobacco mosaic virus on graphite is shown to be consistent with values of lateral friction observed on similar interfaces and the flexural rigidity expected for macromolecular assemblies. The ability to manipulate individual samples broadens the scope of possible studies by providing a means for positioning samples at specific binding sites or predefined measuring devices. The mechanical model provides a framework for interpreting quantitative measurements of virus binding and mechanical properties and for understanding the constraints on the successful, nondestructive AFM manipulation of delicate samples.
Url:
PubMed: 9138585
PubMed Central: 1184522
Links toward previous steps (curation, corpus...)
- to stream Pmc, to step Corpus: Pour aller vers cette notice dans l'étape Curation :000983
Links to Exploration step
PMC:1184522Le document en format XML
<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">Manipulation of individual viruses: friction and mechanical properties.</title><author><name sortKey="Falvo, M R" sort="Falvo, M R" uniqKey="Falvo M" first="M R" last="Falvo">M R Falvo</name></author><author><name sortKey="Washburn, S" sort="Washburn, S" uniqKey="Washburn S" first="S" last="Washburn">S. Washburn</name></author><author><name sortKey="Superfine, R" sort="Superfine, R" uniqKey="Superfine R" first="R" last="Superfine">R. Superfine</name></author><author><name sortKey="Finch, M" sort="Finch, M" uniqKey="Finch M" first="M" last="Finch">M. Finch</name></author><author><name sortKey="Brooks, F P" sort="Brooks, F P" uniqKey="Brooks F" first="F P" last="Brooks">F P Brooks</name></author><author><name sortKey="Chi, V" sort="Chi, V" uniqKey="Chi V" first="V" last="Chi">V. Chi</name></author><author><name sortKey="Taylor, R M" sort="Taylor, R M" uniqKey="Taylor R" first="R M" last="Taylor">R M Taylor</name></author></titleStmt><publicationStmt><idno type="wicri:source">PMC</idno><idno type="pmid">9138585</idno><idno type="pmc">1184522</idno><idno type="url">http://www.ncbi.nlm.nih.gov/pmc/articles/PMC1184522</idno><idno type="RBID">PMC:1184522</idno><date when="1997">1997</date><idno type="wicri:Area/Pmc/Corpus">000983</idno><idno type="wicri:Area/Pmc/Curation">000983</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en" level="a" type="main">Manipulation of individual viruses: friction and mechanical properties.</title><author><name sortKey="Falvo, M R" sort="Falvo, M R" uniqKey="Falvo M" first="M R" last="Falvo">M R Falvo</name></author><author><name sortKey="Washburn, S" sort="Washburn, S" uniqKey="Washburn S" first="S" last="Washburn">S. Washburn</name></author><author><name sortKey="Superfine, R" sort="Superfine, R" uniqKey="Superfine R" first="R" last="Superfine">R. Superfine</name></author><author><name sortKey="Finch, M" sort="Finch, M" uniqKey="Finch M" first="M" last="Finch">M. Finch</name></author><author><name sortKey="Brooks, F P" sort="Brooks, F P" uniqKey="Brooks F" first="F P" last="Brooks">F P Brooks</name></author><author><name sortKey="Chi, V" sort="Chi, V" uniqKey="Chi V" first="V" last="Chi">V. Chi</name></author><author><name sortKey="Taylor, R M" sort="Taylor, R M" uniqKey="Taylor R" first="R M" last="Taylor">R M Taylor</name></author></analytic><series><title level="j">Biophysical Journal</title><idno type="ISSN">0006-3495</idno><idno type="eISSN">1542-0086</idno><imprint><date when="1997">1997</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en"><p>We present our results on the manipulation of individual viruses using an advanced interface for atomic force microscopes (AFMs). We show that the viruses can be dissected, rotated, and translated with great facility. We interpret the behavior of tobacco mosaic virus with a mechanical model that makes explicit the competition between sample-substrate lateral friction and the flexural rigidity of the manipulated object. The manipulation behavior of tobacco mosaic virus on graphite is shown to be consistent with values of lateral friction observed on similar interfaces and the flexural rigidity expected for macromolecular assemblies. The ability to manipulate individual samples broadens the scope of possible studies by providing a means for positioning samples at specific binding sites or predefined measuring devices. The mechanical model provides a framework for interpreting quantitative measurements of virus binding and mechanical properties and for understanding the constraints on the successful, nondestructive AFM manipulation of delicate samples.</p><sec sec-type="scanned-figures"><title>Images</title><fig id="F1"><label>FIGURE 1</label><graphic xlink:href="biophysj00036-0418-a" xlink:role="1398"></graphic></fig><fig id="F2"><label>FIGURE 2</label><graphic xlink:href="biophysj00036-0419-a" xlink:role="1399"></graphic></fig><fig id="F3"><label>FIGURE 3</label><graphic xlink:href="biophysj00036-0419-b" xlink:role="1399"></graphic></fig><fig id="F4"><label>FIGURE 5</label><graphic xlink:href="biophysj00036-0421-a" xlink:role="1401"></graphic></fig></sec></div></front></TEI><pmc article-type="research-article"><pmc-comment>The publisher of this article does not allow downloading of the full text in XML form.</pmc-comment>
<front><journal-meta><journal-id journal-id-type="nlm-ta">Biophys J</journal-id><journal-title>Biophysical Journal</journal-title><issn pub-type="ppub">0006-3495</issn><issn pub-type="epub">1542-0086</issn></journal-meta><article-meta><article-id pub-id-type="pmid">9138585</article-id><article-id pub-id-type="pmc">1184522</article-id><article-categories><subj-group subj-group-type="heading"><subject>Research Article</subject></subj-group></article-categories><title-group><article-title>Manipulation of individual viruses: friction and mechanical properties.</article-title></title-group><contrib-group><contrib contrib-type="author"><name><surname>Falvo</surname><given-names>M R</given-names></name></contrib><contrib contrib-type="author"><name><surname>Washburn</surname><given-names>S</given-names></name></contrib><contrib contrib-type="author"><name><surname>Superfine</surname><given-names>R</given-names></name></contrib><contrib contrib-type="author"><name><surname>Finch</surname><given-names>M</given-names></name></contrib><contrib contrib-type="author"><name><surname>Brooks</surname><given-names>F P</given-names><suffix>Jr</suffix></name></contrib><contrib contrib-type="author"><name><surname>Chi</surname><given-names>V</given-names></name></contrib><contrib contrib-type="author"><name><surname>Taylor</surname><given-names>R M</given-names><suffix>2nd</suffix></name></contrib></contrib-group><aff>Department of Physics and Astronomy, University of North Carolina, Chapel Hill 27599, USA.</aff><pub-date pub-type="ppub"><month>3</month><year>1997</year></pub-date><volume>72</volume><issue>3</issue><fpage>1396</fpage><lpage>1403</lpage><abstract><p>We present our results on the manipulation of individual viruses using an advanced interface for atomic force microscopes (AFMs). We show that the viruses can be dissected, rotated, and translated with great facility. We interpret the behavior of tobacco mosaic virus with a mechanical model that makes explicit the competition between sample-substrate lateral friction and the flexural rigidity of the manipulated object. The manipulation behavior of tobacco mosaic virus on graphite is shown to be consistent with values of lateral friction observed on similar interfaces and the flexural rigidity expected for macromolecular assemblies. The ability to manipulate individual samples broadens the scope of possible studies by providing a means for positioning samples at specific binding sites or predefined measuring devices. The mechanical model provides a framework for interpreting quantitative measurements of virus binding and mechanical properties and for understanding the constraints on the successful, nondestructive AFM manipulation of delicate samples.</p><sec sec-type="scanned-figures"><title>Images</title><fig id="F1"><label>FIGURE 1</label><graphic xlink:href="biophysj00036-0418-a" xlink:role="1398"></graphic></fig><fig id="F2"><label>FIGURE 2</label><graphic xlink:href="biophysj00036-0419-a" xlink:role="1399"></graphic></fig><fig id="F3"><label>FIGURE 3</label><graphic xlink:href="biophysj00036-0419-b" xlink:role="1399"></graphic></fig><fig id="F4"><label>FIGURE 5</label><graphic xlink:href="biophysj00036-0421-a" xlink:role="1401"></graphic></fig></sec></abstract></article-meta></front></pmc></record>Pour manipuler ce document sous Unix (Dilib)
EXPLOR_STEP=$WICRI_ROOT/Ticri/CIDE/explor/HapticV1/Data/Pmc/Curation
HfdSelect -h $EXPLOR_STEP/biblio.hfd -nk 000983 | SxmlIndent | more
Ou
HfdSelect -h $EXPLOR_AREA/Data/Pmc/Curation/biblio.hfd -nk 000983 | SxmlIndent | more
Pour mettre un lien sur cette page dans le réseau Wicri
{{Explor lien
|wiki= Ticri/CIDE
|area= HapticV1
|flux= Pmc
|étape= Curation
|type= RBID
|clé= PMC:1184522
|texte= Manipulation of individual viruses: friction and mechanical properties.
}}
Pour générer des pages wiki
HfdIndexSelect -h $EXPLOR_AREA/Data/Pmc/Curation/RBID.i -Sk "pubmed:9138585" \
| HfdSelect -Kh $EXPLOR_AREA/Data/Pmc/Curation/biblio.hfd \
| NlmPubMed2Wicri -a HapticV1
| This area was generated with Dilib version V0.6.23. |  |