TGFβ-Smad signalling in postoperative human lens epithelial cells
Identifieur interne : 002640 ( Pmc/Checkpoint ); précédent : 002639; suivant : 002641TGFβ-Smad signalling in postoperative human lens epithelial cells
Auteurs : S. Saika [Japon] ; T. Miyamoto [Japon] ; I. Ishida [Japon] ; K. Shirai [Japon] ; Y. Ohnishi [Japon] ; A. Ooshima [Japon] ; J W Mcavoy [Australie]Source :
- The British Journal of Ophthalmology [ 0007-1161 ] ; 2002.
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PubMed: 12446380
PubMed Central: 1771405
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<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">TGFβ-Smad signalling in postoperative human lens epithelial cells</title><author><name sortKey="Saika, S" sort="Saika, S" uniqKey="Saika S" first="S" last="Saika">S. Saika</name><affiliation wicri:level="1"><nlm:aff id="aff1">Department of Ophthalmology, Wakayama Medical University, Wakayama, Japan</nlm:aff><country xml:lang="fr">Japon</country><wicri:regionArea>Department of Ophthalmology, Wakayama Medical University, Wakayama</wicri:regionArea><wicri:noRegion>Wakayama</wicri:noRegion></affiliation></author><author><name sortKey="Miyamoto, T" sort="Miyamoto, T" uniqKey="Miyamoto T" first="T" last="Miyamoto">T. Miyamoto</name><affiliation wicri:level="1"><nlm:aff id="aff1">Department of Ophthalmology, Wakayama Medical University, Wakayama, Japan</nlm:aff><country xml:lang="fr">Japon</country><wicri:regionArea>Department of Ophthalmology, Wakayama Medical University, Wakayama</wicri:regionArea><wicri:noRegion>Wakayama</wicri:noRegion></affiliation></author><author><name sortKey="Ishida, I" sort="Ishida, I" uniqKey="Ishida I" first="I" last="Ishida">I. Ishida</name><affiliation wicri:level="1"><nlm:aff id="aff1">Department of Ophthalmology, Wakayama Medical University, Wakayama, Japan</nlm:aff><country xml:lang="fr">Japon</country><wicri:regionArea>Department of Ophthalmology, Wakayama Medical University, Wakayama</wicri:regionArea><wicri:noRegion>Wakayama</wicri:noRegion></affiliation></author><author><name sortKey="Shirai, K" sort="Shirai, K" uniqKey="Shirai K" first="K" last="Shirai">K. Shirai</name><affiliation wicri:level="1"><nlm:aff id="aff1">Department of Ophthalmology, Wakayama Medical University, Wakayama, Japan</nlm:aff><country xml:lang="fr">Japon</country><wicri:regionArea>Department of Ophthalmology, Wakayama Medical University, Wakayama</wicri:regionArea><wicri:noRegion>Wakayama</wicri:noRegion></affiliation></author><author><name sortKey="Ohnishi, Y" sort="Ohnishi, Y" uniqKey="Ohnishi Y" first="Y" last="Ohnishi">Y. Ohnishi</name><affiliation wicri:level="1"><nlm:aff id="aff1">Department of Ophthalmology, Wakayama Medical University, Wakayama, Japan</nlm:aff><country xml:lang="fr">Japon</country><wicri:regionArea>Department of Ophthalmology, Wakayama Medical University, Wakayama</wicri:regionArea><wicri:noRegion>Wakayama</wicri:noRegion></affiliation></author><author><name sortKey="Ooshima, A" sort="Ooshima, A" uniqKey="Ooshima A" first="A" last="Ooshima">A. Ooshima</name><affiliation wicri:level="1"><nlm:aff id="aff2">Department of Pathology, Wakayama Medical University, Wakayama, Japan</nlm:aff><country xml:lang="fr">Japon</country><wicri:regionArea>Department of Pathology, Wakayama Medical University, Wakayama</wicri:regionArea><wicri:noRegion>Wakayama</wicri:noRegion></affiliation></author><author><name sortKey="Mcavoy, J W" sort="Mcavoy, J W" uniqKey="Mcavoy J" first="J W" last="Mcavoy">J W Mcavoy</name><affiliation wicri:level="1"><nlm:aff id="aff3">Save Sight Institute and Department of Anatomy and Histology, University of Sydney, Sydney, NSW, Australia</nlm:aff><country xml:lang="fr">Australie</country><wicri:regionArea>Save Sight Institute and Department of Anatomy and Histology, University of Sydney, Sydney, NSW</wicri:regionArea><wicri:noRegion>NSW</wicri:noRegion></affiliation></author></titleStmt><publicationStmt><idno type="wicri:source">PMC</idno><idno type="pmid">12446380</idno><idno type="pmc">1771405</idno><idno type="url">http://www.ncbi.nlm.nih.gov/pmc/articles/PMC1771405</idno><idno type="RBID">PMC:1771405</idno><date when="2002">2002</date><idno type="wicri:Area/Pmc/Corpus">000987</idno><idno type="wicri:Area/Pmc/Curation">000987</idno><idno type="wicri:Area/Pmc/Checkpoint">002640</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en" level="a" type="main">TGFβ-Smad signalling in postoperative human lens epithelial cells</title><author><name sortKey="Saika, S" sort="Saika, S" uniqKey="Saika S" first="S" last="Saika">S. Saika</name><affiliation wicri:level="1"><nlm:aff id="aff1">Department of Ophthalmology, Wakayama Medical University, Wakayama, Japan</nlm:aff><country xml:lang="fr">Japon</country><wicri:regionArea>Department of Ophthalmology, Wakayama Medical University, Wakayama</wicri:regionArea><wicri:noRegion>Wakayama</wicri:noRegion></affiliation></author><author><name sortKey="Miyamoto, T" sort="Miyamoto, T" uniqKey="Miyamoto T" first="T" last="Miyamoto">T. Miyamoto</name><affiliation wicri:level="1"><nlm:aff id="aff1">Department of Ophthalmology, Wakayama Medical University, Wakayama, Japan</nlm:aff><country xml:lang="fr">Japon</country><wicri:regionArea>Department of Ophthalmology, Wakayama Medical University, Wakayama</wicri:regionArea><wicri:noRegion>Wakayama</wicri:noRegion></affiliation></author><author><name sortKey="Ishida, I" sort="Ishida, I" uniqKey="Ishida I" first="I" last="Ishida">I. Ishida</name><affiliation wicri:level="1"><nlm:aff id="aff1">Department of Ophthalmology, Wakayama Medical University, Wakayama, Japan</nlm:aff><country xml:lang="fr">Japon</country><wicri:regionArea>Department of Ophthalmology, Wakayama Medical University, Wakayama</wicri:regionArea><wicri:noRegion>Wakayama</wicri:noRegion></affiliation></author><author><name sortKey="Shirai, K" sort="Shirai, K" uniqKey="Shirai K" first="K" last="Shirai">K. Shirai</name><affiliation wicri:level="1"><nlm:aff id="aff1">Department of Ophthalmology, Wakayama Medical University, Wakayama, Japan</nlm:aff><country xml:lang="fr">Japon</country><wicri:regionArea>Department of Ophthalmology, Wakayama Medical University, Wakayama</wicri:regionArea><wicri:noRegion>Wakayama</wicri:noRegion></affiliation></author><author><name sortKey="Ohnishi, Y" sort="Ohnishi, Y" uniqKey="Ohnishi Y" first="Y" last="Ohnishi">Y. Ohnishi</name><affiliation wicri:level="1"><nlm:aff id="aff1">Department of Ophthalmology, Wakayama Medical University, Wakayama, Japan</nlm:aff><country xml:lang="fr">Japon</country><wicri:regionArea>Department of Ophthalmology, Wakayama Medical University, Wakayama</wicri:regionArea><wicri:noRegion>Wakayama</wicri:noRegion></affiliation></author><author><name sortKey="Ooshima, A" sort="Ooshima, A" uniqKey="Ooshima A" first="A" last="Ooshima">A. Ooshima</name><affiliation wicri:level="1"><nlm:aff id="aff2">Department of Pathology, Wakayama Medical University, Wakayama, Japan</nlm:aff><country xml:lang="fr">Japon</country><wicri:regionArea>Department of Pathology, Wakayama Medical University, Wakayama</wicri:regionArea><wicri:noRegion>Wakayama</wicri:noRegion></affiliation></author><author><name sortKey="Mcavoy, J W" sort="Mcavoy, J W" uniqKey="Mcavoy J" first="J W" last="Mcavoy">J W Mcavoy</name><affiliation wicri:level="1"><nlm:aff id="aff3">Save Sight Institute and Department of Anatomy and Histology, University of Sydney, Sydney, NSW, Australia</nlm:aff><country xml:lang="fr">Australie</country><wicri:regionArea>Save Sight Institute and Department of Anatomy and Histology, University of Sydney, Sydney, NSW</wicri:regionArea><wicri:noRegion>NSW</wicri:noRegion></affiliation></author></analytic><series><title level="j">The British Journal of Ophthalmology</title><idno type="ISSN">0007-1161</idno><idno type="eISSN">1468-2079</idno><imprint><date when="2002">2002</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en"><p><bold>Aims:</bold> To localise Smads3/4 proteins in lens epithelial cells (LECs) of fresh and postoperative human specimens. Smads3/4 are involved in signal transduction between transforming growth factor β (TGFβ) cell surface receptors and gene promoters. Nuclear localisation of Smads indicates achievement of endogenous TGFβ signalling in cells.</p><p><bold>Methods:</bold> Three circular sections of the anterior capsule, one lens, and 17 capsules undergoing postoperative healing were studied. Immunohistochemistry was performed for Smads3/4 in paraffin sections of the specimens. The effect of exogenous TGFβ2 on Smad3 subcellular localisation was examined in explant cultures of extracted human anterior lens epithelium.</p><p><bold>Results:</bold> The cytoplasm, but not the nuclei, of LECs of uninjured lenses was immunoreactive for Smads3/4. In contrast, nuclear immunoreactivity for Smads3/4 was detected in LECs during capsular healing. Nuclei positive for Smads3/4 were observed in monolayered LECs adjacent to the regenerated lens fibres of Sommerring’s ring. Interestingly, the nuclei of LECs that were somewhat elongated, and appeared to be differentiating into fibre-like cells, were negative for Smads3/4. Fibroblast-like, spindle-shaped lens cells with nuclear immunoreactivity for nuclear Smads3/4 were occasionally observed in the extracellular matrix accumulated in capsular opacification. Exogenous TGFβ induced nuclear translocation of Smad3 in LECs of anterior capsule specimens in explant culture.</p><p><bold>Conclusions:</bold> This is consistent with TGFβ induced Smad signalling being involved in regulating the behaviour of LECs during wound healing after cataract surgery.</p></div></front></TEI><pmc article-type="research-article"><pmc-comment>The publisher of this article does not allow downloading of the full text in XML form.</pmc-comment>
<front><journal-meta><journal-id journal-id-type="nlm-ta">Br J Ophthalmol</journal-id><journal-id journal-id-type="publisher-id">bjophthalmol</journal-id><journal-title>The British Journal of Ophthalmology</journal-title><issn pub-type="ppub">0007-1161</issn><issn pub-type="epub">1468-2079</issn><publisher><publisher-name>Copyright 2002 British Journal of Ophthalmology</publisher-name></publisher></journal-meta><article-meta><article-id pub-id-type="pmid">12446380</article-id><article-id pub-id-type="pmc">1771405</article-id><article-id pub-id-type="publisher-id">0861428</article-id><article-categories><subj-group subj-group-type="heading"><subject>Laboratory Science</subject></subj-group></article-categories><title-group><article-title>TGFβ-Smad signalling in postoperative human lens epithelial cells</article-title></title-group><contrib-group><contrib contrib-type="author"><name><surname>Saika</surname><given-names>S</given-names></name><xref ref-type="aff" rid="aff1">1</xref></contrib><contrib contrib-type="author"><name><surname>Miyamoto</surname><given-names>T</given-names></name><xref ref-type="aff" rid="aff1">1</xref></contrib><contrib contrib-type="author"><name><surname>Ishida</surname><given-names>I</given-names></name><xref ref-type="aff" rid="aff1">1</xref></contrib><contrib contrib-type="author"><name><surname>Shirai</surname><given-names>K</given-names></name><xref ref-type="aff" rid="aff1">1</xref></contrib><contrib contrib-type="author"><name><surname>Ohnishi</surname><given-names>Y</given-names></name><xref ref-type="aff" rid="aff1">1</xref></contrib><contrib contrib-type="author"><name><surname>Ooshima</surname><given-names>A</given-names></name><xref ref-type="aff" rid="aff2">2</xref></contrib><contrib contrib-type="author"><name><surname>McAvoy</surname><given-names>J W</given-names></name><xref ref-type="aff" rid="aff3">3</xref></contrib></contrib-group><aff id="aff1"><label>1</label>Department of Ophthalmology, Wakayama Medical University, Wakayama, Japan</aff><aff id="aff2"><label>2</label>Department of Pathology, Wakayama Medical University, Wakayama, Japan</aff><aff id="aff3"><label>3</label>Save Sight Institute and Department of Anatomy and Histology, University of Sydney, Sydney, NSW, Australia</aff><author-notes><fn><p>Correspondence to:
Shizuya Saika, MD, PhD, Department of Ophthalmology, Wakayama Medical University, 811-1 Kimiidera, Wakayama, 641-0012, Japan;
<email>shizuya@wakayama-med.ac.jp</email></p></fn></author-notes><pub-date pub-type="ppub"><month>12</month><year>2002</year></pub-date><volume>86</volume><issue>12</issue><fpage>1428</fpage><lpage>1433</lpage><history><date date-type="accepted"><day>17</day><month>7</month><year>2002</year></date></history><copyright-statement>Copyright © Copyright 2002 British Journal of Ophthalmology</copyright-statement><copyright-year>2002</copyright-year><abstract><p><bold>Aims:</bold> To localise Smads3/4 proteins in lens epithelial cells (LECs) of fresh and postoperative human specimens. Smads3/4 are involved in signal transduction between transforming growth factor β (TGFβ) cell surface receptors and gene promoters. Nuclear localisation of Smads indicates achievement of endogenous TGFβ signalling in cells.</p><p><bold>Methods:</bold> Three circular sections of the anterior capsule, one lens, and 17 capsules undergoing postoperative healing were studied. Immunohistochemistry was performed for Smads3/4 in paraffin sections of the specimens. The effect of exogenous TGFβ2 on Smad3 subcellular localisation was examined in explant cultures of extracted human anterior lens epithelium.</p><p><bold>Results:</bold> The cytoplasm, but not the nuclei, of LECs of uninjured lenses was immunoreactive for Smads3/4. In contrast, nuclear immunoreactivity for Smads3/4 was detected in LECs during capsular healing. Nuclei positive for Smads3/4 were observed in monolayered LECs adjacent to the regenerated lens fibres of Sommerring’s ring. Interestingly, the nuclei of LECs that were somewhat elongated, and appeared to be differentiating into fibre-like cells, were negative for Smads3/4. Fibroblast-like, spindle-shaped lens cells with nuclear immunoreactivity for nuclear Smads3/4 were occasionally observed in the extracellular matrix accumulated in capsular opacification. Exogenous TGFβ induced nuclear translocation of Smad3 in LECs of anterior capsule specimens in explant culture.</p><p><bold>Conclusions:</bold> This is consistent with TGFβ induced Smad signalling being involved in regulating the behaviour of LECs during wound healing after cataract surgery.</p></abstract><kwd-group><kwd>lens epithelial cell</kwd><kwd>transforming growth factor β</kwd><kwd>Smad</kwd><kwd>cataract surgery</kwd></kwd-group></article-meta></front></pmc><affiliations><list><country><li>Australie</li><li>Japon</li></country></list><tree><country name="Japon"><noRegion><name sortKey="Saika, S" sort="Saika, S" uniqKey="Saika S" first="S" last="Saika">S. Saika</name></noRegion><name sortKey="Ishida, I" sort="Ishida, I" uniqKey="Ishida I" first="I" last="Ishida">I. Ishida</name><name sortKey="Miyamoto, T" sort="Miyamoto, T" uniqKey="Miyamoto T" first="T" last="Miyamoto">T. Miyamoto</name><name sortKey="Ohnishi, Y" sort="Ohnishi, Y" uniqKey="Ohnishi Y" first="Y" last="Ohnishi">Y. Ohnishi</name><name sortKey="Ooshima, A" sort="Ooshima, A" uniqKey="Ooshima A" first="A" last="Ooshima">A. Ooshima</name><name sortKey="Shirai, K" sort="Shirai, K" uniqKey="Shirai K" first="K" last="Shirai">K. Shirai</name></country><country name="Australie"><noRegion><name sortKey="Mcavoy, J W" sort="Mcavoy, J W" uniqKey="Mcavoy J" first="J W" last="Mcavoy">J W Mcavoy</name></noRegion></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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