Two-photon microscopy as a tool to study blood flow and neurovascular coupling in the rodent brain
Identifieur interne : 002111 ( Main/Merge ); précédent : 002110; suivant : 002112Two-photon microscopy as a tool to study blood flow and neurovascular coupling in the rodent brain
Auteurs : Andy Y. Shih [États-Unis] ; Jonathan D. Driscoll [États-Unis] ; Patrick J. Drew [États-Unis] ; Nozomi Nishimura [États-Unis] ; Chris B. Schaffer [États-Unis] ; David Kleinfeld [États-Unis]Source :
- Journal of Cerebral Blood Flow & Metabolism [ 0271-678X ] ; 2012.
Abstract
The cerebral vascular system services the constant demand for energy during neuronal activity in the brain. Attempts to delineate the logic of neurovascular coupling have been greatly aided by the advent of two-photon laser scanning microscopy to image both blood flow and the activity of individual cells below the surface of the brain. Here we provide a technical guide to imaging cerebral blood flow in rodents. We describe in detail the surgical procedures required to generate cranial windows for optical access to the cortex of both rats and mice and the use of two-photon microscopy to accurately measure blood flow in individual cortical vessels concurrent with local cellular activity. We further provide examples on how these techniques can be applied to the study of local blood flow regulation and vascular pathologies such as small-scale stroke.
Url:
DOI: 10.1038/jcbfm.2011.196
PubMed: 22293983
PubMed Central: 3390800
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PMC:3390800Le document en format XML
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<front><div type="abstract" xml:lang="en"><p>The cerebral vascular system services the constant demand for energy during neuronal activity in the brain. Attempts to delineate the logic of neurovascular coupling have been greatly aided by the advent of two-photon laser scanning microscopy to image both blood flow and the activity of individual cells below the surface of the brain. Here we provide a technical guide to imaging cerebral blood flow in rodents. We describe in detail the surgical procedures required to generate cranial windows for optical access to the cortex of both rats and mice and the use of two-photon microscopy to accurately measure blood flow in individual cortical vessels concurrent with local cellular activity. We further provide examples on how these techniques can be applied to the study of local blood flow regulation and vascular pathologies such as small-scale stroke.</p>
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