Optogenetic Patterning of Whisker-Barrel Cortical System in Transgenic Rat Expressing Channelrhodopsin-2
Identifieur interne : 000F73 ( Main/Merge ); précédent : 000F72; suivant : 000F74Optogenetic Patterning of Whisker-Barrel Cortical System in Transgenic Rat Expressing Channelrhodopsin-2
Auteurs : Tatsuya Honjoh [Japon] ; Zhi-Gang Ji [Japon] ; Yukinobu Yokoyama [Japon] ; Akira Sumiyoshi [Japon] ; Yuma Shibuya [Japon] ; Yoshiya Matsuzaka [Japon] ; Ryuta Kawashima [Japon] ; Hajime Mushiake [Japon] ; Toru Ishizuka [Japon] ; Hiromu Yawo [Japon]Source :
- PLoS ONE [ 1932-6203 ] ; 2014.
Abstract
The rodent whisker-barrel system has been an ideal model for studying somatosensory representations in the cortex. However, it remains a challenge to experimentally stimulate whiskers with a given pattern under spatiotemporal precision. Recently the optogenetic manipulation of neuronal activity has made possible the analysis of the neuronal network with precise spatiotemporal resolution. Here we identified the selective expression of channelrhodopsin-2 (ChR2), an algal light-driven cation channel, in the large mechanoreceptive neurons in the trigeminal ganglion (TG) as well as their peripheral nerve endings innervating the whisker follicles of a transgenic rat. The spatiotemporal pattern of whisker irradiation thus produced a barrel-cortical response with a specific spatiotemporal pattern as evidenced by electrophysiological and functional MRI (fMRI) studies. Our methods of generating an optogenetic tactile pattern (OTP) can be expected to facilitate studies on how the spatiotemporal pattern of touch is represented in the somatosensory cortex, as Hubel and Wiesel did in the visual cortex.
Url:
DOI: 10.1371/journal.pone.0093706
PubMed: 24695456
PubMed Central: 3973546
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xml:lang="en" level="a" type="main">Optogenetic Patterning of Whisker-Barrel Cortical System in Transgenic Rat Expressing Channelrhodopsin-2</title><author><name sortKey="Honjoh, Tatsuya" sort="Honjoh, Tatsuya" uniqKey="Honjoh T" first="Tatsuya" last="Honjoh">Tatsuya Honjoh</name><affiliation wicri:level="1"><nlm:aff id="aff1"><addr-line>Department of Developmental Biology and Neuroscience, Tohoku University Graduate School of Life Sciences, Sendai, Japan</addr-line></nlm:aff><country xml:lang="fr">Japon</country><wicri:regionArea>Department of Developmental Biology and Neuroscience, Tohoku University Graduate School of Life Sciences, Sendai</wicri:regionArea><wicri:noRegion>Sendai</wicri:noRegion></affiliation><affiliation wicri:level="3"><nlm:aff id="aff2"><addr-line>Japan Science and Technology Agency (JST), Core Research of Evolutional Science & Technology (CREST), Tokyo, Japan</addr-line></nlm:aff><country xml:lang="fr">Japon</country><wicri:regionArea>Japan Science and Technology Agency (JST), Core Research of Evolutional Science & Technology (CREST), Tokyo</wicri:regionArea><placeName><settlement type="city">Tokyo</settlement></placeName></affiliation></author><author><name sortKey="Ji, Zhi Gang" sort="Ji, Zhi Gang" uniqKey="Ji Z" first="Zhi-Gang" last="Ji">Zhi-Gang Ji</name><affiliation wicri:level="1"><nlm:aff id="aff1"><addr-line>Department of Developmental Biology and Neuroscience, Tohoku University Graduate School of Life Sciences, Sendai, Japan</addr-line></nlm:aff><country xml:lang="fr">Japon</country><wicri:regionArea>Department of Developmental Biology and Neuroscience, Tohoku University Graduate School of Life Sciences, Sendai</wicri:regionArea><wicri:noRegion>Sendai</wicri:noRegion></affiliation><affiliation wicri:level="3"><nlm:aff id="aff2"><addr-line>Japan Science and Technology Agency (JST), Core Research of Evolutional Science & Technology (CREST), Tokyo, Japan</addr-line></nlm:aff><country xml:lang="fr">Japon</country><wicri:regionArea>Japan Science and Technology Agency (JST), Core Research of Evolutional Science & Technology (CREST), Tokyo</wicri:regionArea><placeName><settlement type="city">Tokyo</settlement></placeName></affiliation></author><author><name sortKey="Yokoyama, Yukinobu" sort="Yokoyama, Yukinobu" uniqKey="Yokoyama Y" first="Yukinobu" last="Yokoyama">Yukinobu Yokoyama</name><affiliation wicri:level="1"><nlm:aff id="aff1"><addr-line>Department of Developmental Biology and Neuroscience, Tohoku University Graduate School of Life Sciences, Sendai, Japan</addr-line></nlm:aff><country xml:lang="fr">Japon</country><wicri:regionArea>Department of Developmental Biology and Neuroscience, Tohoku University Graduate School of Life Sciences, Sendai</wicri:regionArea><wicri:noRegion>Sendai</wicri:noRegion></affiliation><affiliation wicri:level="3"><nlm:aff id="aff2"><addr-line>Japan Science and Technology Agency (JST), Core Research of Evolutional Science & Technology (CREST), Tokyo, Japan</addr-line></nlm:aff><country xml:lang="fr">Japon</country><wicri:regionArea>Japan Science and Technology Agency (JST), Core Research of Evolutional Science & Technology (CREST), Tokyo</wicri:regionArea><placeName><settlement type="city">Tokyo</settlement></placeName></affiliation></author><author><name sortKey="Sumiyoshi, Akira" sort="Sumiyoshi, Akira" uniqKey="Sumiyoshi A" first="Akira" last="Sumiyoshi">Akira Sumiyoshi</name><affiliation wicri:level="4"><nlm:aff id="aff3"><addr-line>Institute of Development, Aging and Cancer, Tohoku University, Sendai, Japan</addr-line></nlm:aff><country xml:lang="fr">Japon</country><wicri:regionArea>Institute of Development, Aging and Cancer, Tohoku University, Sendai</wicri:regionArea><orgName type="university">Université du Tōhoku</orgName><placeName><settlement type="city">Sendai</settlement><region type="province">Région de Tōhoku</region></placeName></affiliation></author><author><name sortKey="Shibuya, Yuma" sort="Shibuya, Yuma" uniqKey="Shibuya Y" first="Yuma" last="Shibuya">Yuma Shibuya</name><affiliation wicri:level="1"><nlm:aff id="aff1"><addr-line>Department of Developmental Biology and Neuroscience, Tohoku University Graduate School of Life Sciences, Sendai, Japan</addr-line></nlm:aff><country xml:lang="fr">Japon</country><wicri:regionArea>Department of Developmental Biology and Neuroscience, Tohoku University Graduate School of Life Sciences, Sendai</wicri:regionArea><wicri:noRegion>Sendai</wicri:noRegion></affiliation></author><author><name sortKey="Matsuzaka, Yoshiya" sort="Matsuzaka, Yoshiya" uniqKey="Matsuzaka Y" first="Yoshiya" last="Matsuzaka">Yoshiya Matsuzaka</name><affiliation wicri:level="3"><nlm:aff id="aff2"><addr-line>Japan Science and Technology Agency (JST), Core Research of Evolutional Science & Technology (CREST), Tokyo, Japan</addr-line></nlm:aff><country xml:lang="fr">Japon</country><wicri:regionArea>Japan Science and Technology Agency (JST), Core Research of Evolutional Science & Technology (CREST), Tokyo</wicri:regionArea><placeName><settlement type="city">Tokyo</settlement></placeName></affiliation><affiliation wicri:level="4"><nlm:aff id="aff4"><addr-line>Department of Physiology, Tohoku University, Graduate School of Medicine, Sendai, Japan</addr-line></nlm:aff><country xml:lang="fr">Japon</country><wicri:regionArea>Department of Physiology, Tohoku University, Graduate School of Medicine, Sendai</wicri:regionArea><orgName type="university">Université du Tōhoku</orgName><placeName><settlement type="city">Sendai</settlement><region type="province">Région de Tōhoku</region></placeName></affiliation></author><author><name sortKey="Kawashima, Ryuta" sort="Kawashima, Ryuta" uniqKey="Kawashima R" first="Ryuta" last="Kawashima">Ryuta Kawashima</name><affiliation wicri:level="4"><nlm:aff id="aff3"><addr-line>Institute of Development, Aging and Cancer, Tohoku University, Sendai, Japan</addr-line></nlm:aff><country xml:lang="fr">Japon</country><wicri:regionArea>Institute of Development, Aging and Cancer, Tohoku University, Sendai</wicri:regionArea><orgName type="university">Université du Tōhoku</orgName><placeName><settlement type="city">Sendai</settlement><region type="province">Région de Tōhoku</region></placeName></affiliation></author><author><name sortKey="Mushiake, Hajime" sort="Mushiake, Hajime" uniqKey="Mushiake H" first="Hajime" last="Mushiake">Hajime Mushiake</name><affiliation wicri:level="3"><nlm:aff id="aff2"><addr-line>Japan Science and Technology Agency (JST), Core Research of Evolutional Science & Technology (CREST), Tokyo, Japan</addr-line></nlm:aff><country xml:lang="fr">Japon</country><wicri:regionArea>Japan Science and Technology Agency (JST), Core Research of Evolutional Science & Technology (CREST), Tokyo</wicri:regionArea><placeName><settlement type="city">Tokyo</settlement></placeName></affiliation><affiliation wicri:level="4"><nlm:aff id="aff4"><addr-line>Department of Physiology, Tohoku University, Graduate School of Medicine, Sendai, Japan</addr-line></nlm:aff><country xml:lang="fr">Japon</country><wicri:regionArea>Department of Physiology, Tohoku University, Graduate School of Medicine, Sendai</wicri:regionArea><orgName type="university">Université du Tōhoku</orgName><placeName><settlement type="city">Sendai</settlement><region type="province">Région de Tōhoku</region></placeName></affiliation><affiliation wicri:level="1"><nlm:aff id="aff5"><addr-line>Center for Neuroscience, Tohoku University Graduate School of Medicine, Sendai, Japan</addr-line></nlm:aff><country xml:lang="fr">Japon</country><wicri:regionArea>Center for Neuroscience, Tohoku University Graduate School of Medicine, Sendai</wicri:regionArea><wicri:noRegion>Sendai</wicri:noRegion></affiliation></author><author><name sortKey="Ishizuka, Toru" sort="Ishizuka, Toru" uniqKey="Ishizuka T" first="Toru" last="Ishizuka">Toru Ishizuka</name><affiliation wicri:level="1"><nlm:aff id="aff1"><addr-line>Department of Developmental Biology and Neuroscience, Tohoku University Graduate School of Life Sciences, Sendai, Japan</addr-line></nlm:aff><country xml:lang="fr">Japon</country><wicri:regionArea>Department of Developmental Biology and Neuroscience, Tohoku University Graduate School of Life Sciences, Sendai</wicri:regionArea><wicri:noRegion>Sendai</wicri:noRegion></affiliation><affiliation wicri:level="3"><nlm:aff id="aff2"><addr-line>Japan Science and Technology Agency (JST), Core Research of Evolutional Science & Technology (CREST), Tokyo, Japan</addr-line></nlm:aff><country xml:lang="fr">Japon</country><wicri:regionArea>Japan Science and Technology Agency (JST), Core Research of Evolutional Science & Technology (CREST), Tokyo</wicri:regionArea><placeName><settlement type="city">Tokyo</settlement></placeName></affiliation></author><author><name sortKey="Yawo, Hiromu" sort="Yawo, Hiromu" uniqKey="Yawo H" first="Hiromu" last="Yawo">Hiromu Yawo</name><affiliation wicri:level="1"><nlm:aff id="aff1"><addr-line>Department of Developmental Biology and Neuroscience, Tohoku University Graduate School of Life Sciences, Sendai, Japan</addr-line></nlm:aff><country xml:lang="fr">Japon</country><wicri:regionArea>Department of Developmental Biology and Neuroscience, Tohoku University Graduate School of Life Sciences, Sendai</wicri:regionArea><wicri:noRegion>Sendai</wicri:noRegion></affiliation><affiliation wicri:level="3"><nlm:aff id="aff2"><addr-line>Japan Science and Technology Agency (JST), Core Research of Evolutional Science & Technology (CREST), Tokyo, Japan</addr-line></nlm:aff><country xml:lang="fr">Japon</country><wicri:regionArea>Japan Science and Technology Agency (JST), Core Research of Evolutional Science & Technology (CREST), Tokyo</wicri:regionArea><placeName><settlement type="city">Tokyo</settlement></placeName></affiliation><affiliation wicri:level="1"><nlm:aff id="aff5"><addr-line>Center for Neuroscience, Tohoku University Graduate School of Medicine, Sendai, Japan</addr-line></nlm:aff><country xml:lang="fr">Japon</country><wicri:regionArea>Center for Neuroscience, Tohoku University Graduate School of Medicine, Sendai</wicri:regionArea><wicri:noRegion>Sendai</wicri:noRegion></affiliation></author></analytic><series><title level="j">PLoS ONE</title><idno type="eISSN">1932-6203</idno><imprint><date when="2014">2014</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en"><p>The rodent whisker-barrel system has been an ideal model for studying somatosensory representations in the cortex. However, it remains a challenge to experimentally stimulate whiskers with a given pattern under spatiotemporal precision. Recently the optogenetic manipulation of neuronal activity has made possible the analysis of the neuronal network with precise spatiotemporal resolution. Here we identified the selective expression of channelrhodopsin-2 (ChR2), an algal light-driven cation channel, in the large mechanoreceptive neurons in the trigeminal ganglion (TG) as well as their peripheral nerve endings innervating the whisker follicles of a transgenic rat. The spatiotemporal pattern of whisker irradiation thus produced a barrel-cortical response with a specific spatiotemporal pattern as evidenced by electrophysiological and functional MRI (fMRI) studies. Our methods of generating an optogenetic tactile pattern (OTP) can be expected to facilitate studies on how the spatiotemporal pattern of touch is represented in the somatosensory cortex, as Hubel and Wiesel did in the visual cortex.</p></div></front><back><div1 type="bibliography"><listBibl><biblStruct><analytic><author><name sortKey="Petersen, Cc" uniqKey="Petersen C">CC Petersen</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Knutsen, Pm" uniqKey="Knutsen P">PM Knutsen</name></author><author><name sortKey="Ahissar, E" uniqKey="Ahissar E">E Ahissar</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Diamond, Me" uniqKey="Diamond M">ME Diamond</name></author><author><name sortKey="Von Heimendahl, M" uniqKey="Von Heimendahl M">M von Heimendahl</name></author><author><name sortKey="Knutsen, Pm" uniqKey="Knutsen P">PM Knutsen</name></author><author><name sortKey="Kleinfeld, D" uniqKey="Kleinfeld D">D Kleinfeld</name></author><author><name sortKey="Ahissar, E" uniqKey="Ahissar E">E Ahissar</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Tomita, H" uniqKey="Tomita H">H Tomita</name></author><author><name sortKey="Sugano, E" uniqKey="Sugano E">E Sugano</name></author><author><name sortKey="Fukazawa, Y" uniqKey="Fukazawa Y">Y Fukazawa</name></author><author><name sortKey="Isago, H" uniqKey="Isago H">H Isago</name></author><author><name sortKey="Sugiyama, Y" uniqKey="Sugiyama Y">Y Sugiyama</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Ji, Zg" uniqKey="Ji Z">ZG Ji</name></author><author><name sortKey="Ito, S" uniqKey="Ito S">S Ito</name></author><author><name sortKey="Honjoh, T" uniqKey="Honjoh T">T Honjoh</name></author><author><name sortKey="Ohta, H" uniqKey="Ohta H">H Ohta</name></author><author><name sortKey="Ishizuka, T" uniqKey="Ishizuka T">T Ishizuka</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Honjoh, T" uniqKey="Honjoh T">T Honjoh</name></author><author><name sortKey="Sumiyoshi, A" uniqKey="Sumiyoshi A">A Sumiyoshi</name></author><author><name sortKey="Yokoyama, Y" uniqKey="Yokoyama Y">Y Yokoyama</name></author><author><name sortKey="Ji, Zg" uniqKey="Ji Z">ZG Ji</name></author><author><name sortKey="Ishizuka, T" uniqKey="Ishizuka T">T Ishizuka</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Rice, Fl" uniqKey="Rice F">FL Rice</name></author><author><name sortKey="Fundin, Bt" uniqKey="Fundin B">BT Fundin</name></author><author><name sortKey="Arvidsson, J" uniqKey="Arvidsson J">J Arvidsson</name></author><author><name sortKey="Aldskoqius, H" uniqKey="Aldskoqius H">H Aldskoqius</name></author><author><name sortKey="Johansson, O" uniqKey="Johansson O">O Johansson</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Ichikawa, H" uniqKey="Ichikawa H">H Ichikawa</name></author><author><name sortKey="Sugimoto, T" uniqKey="Sugimoto T">T Sugimoto</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Reinohl, J" uniqKey="Reinohl J">J Reinöhl</name></author><author><name sortKey="Hoheisel, U" uniqKey="Hoheisel U">U Hoheisel</name></author><author><name sortKey="Unger, T" uniqKey="Unger T">T Unger</name></author><author><name sortKey="Mense, S" uniqKey="Mense S">S Mense</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Ebara, S" uniqKey="Ebara S">S Ebara</name></author><author><name sortKey="Kumamoto, K" uniqKey="Kumamoto K">K Kumamoto</name></author><author><name sortKey="Matsuura, T" uniqKey="Matsuura T">T Matsuura</name></author><author><name sortKey="Mazurkiewicz, Je" uniqKey="Mazurkiewicz J">JE Mazurkiewicz</name></author><author><name sortKey="Rice, Fl" uniqKey="Rice F">FL Rice</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Catacuzzeno, L" uniqKey="Catacuzzeno L">L Catacuzzeno</name></author><author><name sortKey="Fioretti, B" uniqKey="Fioretti B">B Fioretti</name></author><author><name sortKey="Pietrobon, D" uniqKey="Pietrobon D">D Pietrobon</name></author><author><name sortKey="Franciolini, F" uniqKey="Franciolini F">F Franciolini</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Villiere, V" uniqKey="Villiere V">V Villière</name></author><author><name sortKey="Mclachlan, Em" uniqKey="Mclachlan E">EM McLachlan</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Hu, J" uniqKey="Hu J">J Hu</name></author><author><name sortKey="Lewin, Gr" uniqKey="Lewin G">GR Lewin</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Coste, B" uniqKey="Coste B">B Coste</name></author><author><name sortKey="Xiao, B" uniqKey="Xiao B">B Xiao</name></author><author><name sortKey="Santos, Js" uniqKey="Santos J">JS Santos</name></author><author><name sortKey="Syeda, R" uniqKey="Syeda R">R Syeda</name></author><author><name sortKey="Grandl, J" uniqKey="Grandl J">J Grandl</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Liu, L" uniqKey="Liu L">L Liu</name></author><author><name sortKey="Oortgiesen, M" uniqKey="Oortgiesen M">M Oortgiesen</name></author><author><name sortKey="Li, L" uniqKey="Li L">L Li</name></author><author><name sortKey="Simon, Sa" uniqKey="Simon S">SA Simon</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Cabanes, C" uniqKey="Cabanes C">C Cabanes</name></author><author><name sortKey="L Pez De Armentia, M" uniqKey="L Pez De Armentia M">M López de Armentia</name></author><author><name sortKey="Viana, F" uniqKey="Viana F">F Viana</name></author><author><name sortKey="Belmonte, C" uniqKey="Belmonte C">C Belmonte</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Xu, S" uniqKey="Xu S">S Xu</name></author><author><name sortKey="Ono, K" uniqKey="Ono K">K Ono</name></author><author><name sortKey="Inenaga, K" uniqKey="Inenaga K">K Inenaga</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Piao, L" uniqKey="Piao L">L Piao</name></author><author><name sortKey="Lee, H" uniqKey="Lee H">H Lee</name></author><author><name sortKey="Park, Ck" uniqKey="Park C">CK Park</name></author><author><name sortKey="Cho, Ih" uniqKey="Cho I">IH Cho</name></author><author><name sortKey="Piao, Zg" uniqKey="Piao Z">ZG Piao</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Catacuzzeno, L" uniqKey="Catacuzzeno L">L Catacuzzeno</name></author><author><name sortKey="Fioretti, B" uniqKey="Fioretti B">B Fioretti</name></author><author><name sortKey="Pietrobon, D" uniqKey="Pietrobon D">D Pietrobon</name></author><author><name sortKey="Franciolini, F" uniqKey="Franciolini F">F Franciolini</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Ooi, L" uniqKey="Ooi L">L Ooi</name></author><author><name sortKey="Gigout, S" uniqKey="Gigout S">S Gigout</name></author><author><name sortKey="Pettinger, L" uniqKey="Pettinger L">L Pettinger</name></author><author><name sortKey="Gamper, N" uniqKey="Gamper N">N Gamper</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Bean, Bp" uniqKey="Bean B">BP Bean</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Boyden, Es" uniqKey="Boyden E">ES Boyden</name></author><author><name sortKey="Zhang, F" uniqKey="Zhang F">F Zhang</name></author><author><name sortKey="Bamberg, E" uniqKey="Bamberg E">E Bamberg</name></author><author><name sortKey="Nagel, G" uniqKey="Nagel G">G Nagel</name></author><author><name sortKey="Deisseroth, K" uniqKey="Deisseroth K">K Deisseroth</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Ishizuka, T" uniqKey="Ishizuka T">T Ishizuka</name></author><author><name sortKey="Kakuda, M" uniqKey="Kakuda M">M Kakuda</name></author><author><name sortKey="Araki, R" uniqKey="Araki R">R Araki</name></author><author><name sortKey="Yawo, H" uniqKey="Yawo H">H Yawo</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Armstrong James, M" uniqKey="Armstrong James M">M Armstrong-James</name></author><author><name sortKey="Fox, K" uniqKey="Fox K">K Fox</name></author><author><name sortKey="Das Gupta, A" uniqKey="Das Gupta A">A Das-Gupta</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Ikezoe, K" uniqKey="Ikezoe K">K Ikezoe</name></author><author><name sortKey="Tamura, H" uniqKey="Tamura H">H Tamura</name></author><author><name sortKey="Kimura, F" uniqKey="Kimura F">F Kimura</name></author><author><name sortKey="Fujita, I" uniqKey="Fujita I">I Fujita</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Ahissar, E" uniqKey="Ahissar E">E Ahissar</name></author><author><name sortKey="Sosnik, R" uniqKey="Sosnik R">R Sosnik</name></author><author><name sortKey="Haidarliu, S" uniqKey="Haidarliu S">S Haidarliu</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Hirata, A" uniqKey="Hirata A">A Hirata</name></author><author><name sortKey="Castro Alamancos, Ma" uniqKey="Castro Alamancos M">MA Castro-Alamancos</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Viaene, An" uniqKey="Viaene A">AN Viaene</name></author><author><name sortKey="Petrof, I" uniqKey="Petrof I">I Petrof</name></author><author><name sortKey="Sherman, Sm" uniqKey="Sherman S">SM Sherman</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Erchova, Ia" uniqKey="Erchova I">IA Erchova</name></author><author><name sortKey="Lebedev, Ma" uniqKey="Lebedev M">MA Lebedev</name></author><author><name sortKey="Diamond, Me" uniqKey="Diamond M">ME Diamond</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Timofeev, I" uniqKey="Timofeev I">I Timofeev</name></author><author><name sortKey="Chauvette, S" uniqKey="Chauvette S">S Chauvette</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Steriade, M" uniqKey="Steriade M">M Steriade</name></author><author><name sortKey="Nu Ez, A" uniqKey="Nu Ez A">A Nuñez</name></author><author><name sortKey="Amzica, F" uniqKey="Amzica F">F Amzica</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Steriade, M" uniqKey="Steriade M">M Steriade</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Ushimaru, M" uniqKey="Ushimaru M">M Ushimaru</name></author><author><name sortKey="Ueta, Y" uniqKey="Ueta Y">Y Ueta</name></author><author><name sortKey="Kawaguchi, Y" uniqKey="Kawaguchi Y">Y Kawaguchi</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Petersen, Cc" uniqKey="Petersen C">CC Petersen</name></author><author><name sortKey="Hahn, Tt" uniqKey="Hahn T">TT Hahn</name></author><author><name sortKey="Mehta, M" uniqKey="Mehta M">M Mehta</name></author><author><name sortKey="Grinvald, A" uniqKey="Grinvald A">A Grinvald</name></author><author><name sortKey="Sakmann, B" uniqKey="Sakmann B">B Sakmann</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Sachdev, Rns" uniqKey="Sachdev R">RNS Sachdev</name></author><author><name sortKey="Ebner, Ff" uniqKey="Ebner F">FF Ebner</name></author><author><name sortKey="Wilson, Cj" uniqKey="Wilson C">CJ Wilson</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Civillico, Ef" uniqKey="Civillico E">EF Civillico</name></author><author><name sortKey="Contreras, D" uniqKey="Contreras D">D Contreras</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Hasenstaub, A" uniqKey="Hasenstaub A">A Hasenstaub</name></author><author><name sortKey="Sachdev, Rns" uniqKey="Sachdev R">RNS Sachdev</name></author><author><name sortKey="Mccormick, Da" uniqKey="Mccormick D">DA McCormick</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Kuki, T" uniqKey="Kuki T">T Kuki</name></author><author><name sortKey="Ohshiro, T" uniqKey="Ohshiro T">T Ohshiro</name></author><author><name sortKey="Ito, S" uniqKey="Ito S">S Ito</name></author><author><name sortKey="Ji, Zg" uniqKey="Ji Z">ZG Ji</name></author><author><name sortKey="Fukazawa, Y" uniqKey="Fukazawa Y">Y Fukazawa</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Watson, Bo" uniqKey="Watson B">BO Watson</name></author><author><name sortKey="Maclean, Jn" uniqKey="Maclean J">JN MacLean</name></author><author><name sortKey="Yuste, R" uniqKey="Yuste R">R Yuste</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Yang, X" uniqKey="Yang X">X Yang</name></author><author><name sortKey="Hyder, F" uniqKey="Hyder F">F Hyder</name></author><author><name sortKey="Shulman, Rg" uniqKey="Shulman R">RG Shulman</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Sanganahalli, Bg" uniqKey="Sanganahalli B">BG Sanganahalli</name></author><author><name sortKey="Herman, P" uniqKey="Herman P">P Herman</name></author><author><name sortKey="Hyder, F" uniqKey="Hyder F">F Hyder</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Yu, X" uniqKey="Yu X">X Yu</name></author><author><name sortKey="Wang, S" uniqKey="Wang S">S Wang</name></author><author><name sortKey="Chen, Dy" uniqKey="Chen D">DY Chen</name></author><author><name sortKey="Dodd, S" uniqKey="Dodd S">S Dodd</name></author><author><name sortKey="Goloshevsky, A" uniqKey="Goloshevsky A">A Goloshevsky</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Logothetis, Nk" uniqKey="Logothetis N">NK Logothetis</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Martin, C" uniqKey="Martin C">C Martin</name></author><author><name sortKey="Martindale, J" uniqKey="Martindale J">J Martindale</name></author><author><name sortKey="Berwick, J" uniqKey="Berwick J">J Berwick</name></author><author><name sortKey="Mayhew, J" uniqKey="Mayhew J">J Mayhew</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Goense, J" uniqKey="Goense J">J Goense</name></author><author><name sortKey="Merkle, H" uniqKey="Merkle H">H Merkle</name></author><author><name sortKey="Logothetis, K" uniqKey="Logothetis K">K Logothetis</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Sumiyoshi, A" uniqKey="Sumiyoshi A">A Sumiyoshi</name></author><author><name sortKey="Suzuki, H" uniqKey="Suzuki H">H Suzuki</name></author><author><name sortKey="Ogawa, T" uniqKey="Ogawa T">T Ogawa</name></author><author><name sortKey="Riera, Jj" uniqKey="Riera J">JJ Riera</name></author><author><name sortKey="Shimokawa, H" uniqKey="Shimokawa H">H Shimokawa</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Desai, M" uniqKey="Desai M">M Desai</name></author><author><name sortKey="Kahn, I" uniqKey="Kahn I">I Kahn</name></author><author><name sortKey="Knoblich, U" uniqKey="Knoblich U">U Knoblich</name></author><author><name sortKey="Bernstein, J" uniqKey="Bernstein J">J Bernstein</name></author><author><name sortKey="Atallah, H" uniqKey="Atallah H">H Atallah</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Kahn, I" uniqKey="Kahn I">I Kahn</name></author><author><name sortKey="Desai, M" uniqKey="Desai M">M Desai</name></author><author><name sortKey="Knoblich, U" uniqKey="Knoblich U">U Knoblich</name></author><author><name sortKey="Bernstein, J" uniqKey="Bernstein J">J Bernstein</name></author><author><name sortKey="Henninger, M" uniqKey="Henninger M">M Henninger</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Abe, Y" uniqKey="Abe Y">Y Abe</name></author><author><name sortKey="Sekino, M" uniqKey="Sekino M">M Sekino</name></author><author><name sortKey="Terazono, Y" uniqKey="Terazono Y">Y Terazono</name></author><author><name sortKey="Ohsaki, H" uniqKey="Ohsaki H">H Ohsaki</name></author><author><name sortKey="Fukazawa, Y" uniqKey="Fukazawa Y">Y Fukazawa</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Simons, Dj" uniqKey="Simons D">DJ Simons</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Knutsen, Pm" uniqKey="Knutsen P">PM Knutsen</name></author><author><name sortKey="Piet, M" uniqKey="Piet M">M Piet</name></author><author><name sortKey="Ahissar, E" uniqKey="Ahissar E">E Ahissar</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="O Connor, Dh" uniqKey="O Connor D">DH O'Connor</name></author><author><name sortKey="Clack, Ng" uniqKey="Clack N">NG Clack</name></author><author><name sortKey="Huber, D" uniqKey="Huber D">D Huber</name></author><author><name sortKey="Komiyama, T" uniqKey="Komiyama T">T Komiyama</name></author><author><name sortKey="Myers, Ew" uniqKey="Myers E">EW Myers</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Petreanu, L" uniqKey="Petreanu L">L Petreanu</name></author><author><name sortKey="Gutnisky, Da" uniqKey="Gutnisky D">DA Gutnisky</name></author><author><name sortKey="Huber, D" uniqKey="Huber D">D Huber</name></author><author><name sortKey="Xu, Nl" uniqKey="Xu N">NL Xu</name></author><author><name sortKey="O Connor, Dh" uniqKey="O Connor D">DH O'Connor</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Hubel, Dh" uniqKey="Hubel D">DH Hubel</name></author><author><name sortKey="Wiesel, Tn" uniqKey="Wiesel T">TN Wiesel</name></author></analytic></biblStruct><biblStruct></biblStruct><biblStruct><analytic><author><name sortKey="Tamamaki, N" uniqKey="Tamamaki N">N Tamamaki</name></author><author><name sortKey="Nakamura, K" uniqKey="Nakamura K">K Nakamura</name></author><author><name sortKey="Furuta, T" uniqKey="Furuta T">T Furuta</name></author><author><name sortKey="Asamoto, K" uniqKey="Asamoto K">K Asamoto</name></author><author><name sortKey="Kaneko, T" uniqKey="Kaneko T">T Kaneko</name></author></analytic></biblStruct></listBibl></div1></back></TEI></record>Pour manipuler ce document sous Unix (Dilib)
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