Atomic force microscopy-based single-cell indentation: Experimentation and finite element simulation
Identifieur interne : 003F69 ( Main/Exploration ); précédent : 003F68; suivant : 003F70Atomic force microscopy-based single-cell indentation: Experimentation and finite element simulation
Auteurs : Hamid Ladjal [France] ; Jean-Luc Hanus [France] ; Anand Pillarisetti [France] ; Carol Keefer [France] ; A. Ferreira [France] ; Jaydev Desai [France]Source :
Descripteurs français
Abstract
In order to understand and characterize the mechanical property and response of the mouse embryonic stem cells (mESC), we used an atomic force microscope (AFM) combined with a PHANToM haptic feedback device. Atomic force microscopy has rapidly become a valuable tool for quantifying the biophysical properties of single cells or a collection of cells through force measurements. We report herein the mechanical characterization of single mESC using indentation-relaxation measurements with micro-sphere AFM probes for fixed and live undifferentiated mESC. During cell indentation for both live and fixed undifferentiated cells, we provided force feedback to the user in real-time through the PHANToM haptic feedback device as the AFM tip was deforming the cell. The force was amplified for the human operator to perceive the change in force during cell indentation by the AFM cantilever. This information can be used as a mechanical marker to characterize state of the cell (live and fixed). As the interpretation of atomic force microscopy-based indentation tests is highly dependent on the use of an appropriate theoretical model of the testing configuration, various contact models are presented to predict the mechanical behavior of an individual mouse embryonic stem cells (mESC) in different states. A comparison study with finite element simulations (FEM) of spherical tip indentation demonstrates the effectiveness of our computational model to predict the mESC deformation during indentation and relaxation nanomanipulation tasks.
Url:
DOI: 10.1109/IROS.2009.5354351
Affiliations:
- France
- Auvergne-Rhône-Alpes, Centre-Val de Loire, Rhône-Alpes, Région Centre
- Lyon, Orléans
- Centre Val de Loire Université, Université Claude Bernard Lyon1, Université d'Orléans, Université de Lyon
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Le document en format XML
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69134 Ecully cedex</addrLine><country key="FR"></country></address><ref type="url">http://www.ec-lyon.fr</ref></desc></org></tutelle><tutelle active="#struct-194495" type="direct"><org type="institution" xml:id="struct-194495" status="VALID"><orgName>Université Claude Bernard Lyon 1</orgName><orgName type="acronym">UCBL</orgName><desc><address><addrLine>43, boulevard du 11 novembre 1918, 69622 Villeurbanne cedex</addrLine><country key="FR"></country></address><ref type="url">http://www.univ-lyon1.fr/</ref></desc></org></tutelle><tutelle name="- LYON" active="#struct-301232" type="direct"><org type="institution" xml:id="struct-301232" status="VALID"><orgName>Institut National des Sciences Appliquées</orgName><orgName type="acronym">INSA</orgName><desc><address><country key="FR"></country></address></desc></org></tutelle><tutelle name="UMR5205" active="#struct-441569" type="direct"><org type="institution" xml:id="struct-441569" status="VALID"><idno type="ISNI">0000000122597504</idno><idno type="IdRef">02636817X</idno><orgName>Centre National de la Recherche Scientifique</orgName><orgName type="acronym">CNRS</orgName><date type="start">1939-10-19</date><desc><address><country key="FR"></country></address><ref type="url">http://www.cnrs.fr/</ref></desc></org></tutelle></tutelles></hal:affiliation><country>France</country><placeName><settlement type="city">Lyon</settlement><region type="region" nuts="2">Auvergne-Rhône-Alpes</region><region type="old region" nuts="2">Rhône-Alpes</region></placeName><orgName type="university">Université Claude Bernard Lyon1</orgName><orgName type="institution" wicri:auto="newGroup">Université de Lyon</orgName></affiliation></author><author><name sortKey="Keefer, Carol" sort="Keefer, Carol" uniqKey="Keefer C" first="Carol" last="Keefer">Carol Keefer</name><affiliation wicri:level="1"><hal:affiliation type="laboratory" xml:id="struct-2003" status="VALID"><orgName>Laboratoire d'InfoRmatique en Image et Systèmes d'information</orgName><orgName type="acronym">LIRIS</orgName><desc><address><addrLine>Bâtiment Blaise Pascal - 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69134 Ecully cedex</addrLine><country key="FR"></country></address><ref type="url">http://www.ec-lyon.fr</ref></desc></org></tutelle><tutelle active="#struct-194495" type="direct"><org type="institution" xml:id="struct-194495" status="VALID"><orgName>Université Claude Bernard Lyon 1</orgName><orgName type="acronym">UCBL</orgName><desc><address><addrLine>43, boulevard du 11 novembre 1918, 69622 Villeurbanne cedex</addrLine><country key="FR"></country></address><ref type="url">http://www.univ-lyon1.fr/</ref></desc></org></tutelle><tutelle name="- LYON" active="#struct-301232" type="direct"><org type="institution" xml:id="struct-301232" status="VALID"><orgName>Institut National des Sciences Appliquées</orgName><orgName type="acronym">INSA</orgName><desc><address><country key="FR"></country></address></desc></org></tutelle><tutelle name="UMR5205" active="#struct-441569" type="direct"><org type="institution" xml:id="struct-441569" status="VALID"><idno type="ISNI">0000000122597504</idno><idno type="IdRef">02636817X</idno><orgName>Centre National de la Recherche Scientifique</orgName><orgName type="acronym">CNRS</orgName><date type="start">1939-10-19</date><desc><address><country key="FR"></country></address><ref type="url">http://www.cnrs.fr/</ref></desc></org></tutelle></tutelles></hal:affiliation><country>France</country><placeName><settlement type="city">Lyon</settlement><region type="region" nuts="2">Auvergne-Rhône-Alpes</region><region type="old region" nuts="2">Rhône-Alpes</region></placeName><orgName type="university">Université Claude Bernard Lyon1</orgName><orgName type="institution" wicri:auto="newGroup">Université de Lyon</orgName></affiliation></author><author><name sortKey="Ferreira, A" sort="Ferreira, A" uniqKey="Ferreira A" first="A." last="Ferreira">A. Ferreira</name><affiliation wicri:level="1"><hal:affiliation type="laboratory" xml:id="struct-2003" status="VALID"><orgName>Laboratoire d'InfoRmatique en Image et Systèmes d'information</orgName><orgName type="acronym">LIRIS</orgName><desc><address><addrLine>Bâtiment Blaise Pascal - 20, avenue Albert Einstein - 69621 Villeurbanne cedex</addrLine><country key="FR"></country></address><ref type="url">http://liris.cnrs.fr/</ref></desc><listRelation><relation active="#struct-33804" type="direct"></relation><relation active="#struct-126765" type="direct"></relation><relation active="#struct-194495" type="direct"></relation><relation name="- LYON" active="#struct-301232" type="direct"></relation><relation name="UMR5205" active="#struct-441569" type="direct"></relation></listRelation><tutelles><tutelle active="#struct-33804" type="direct"><org type="institution" xml:id="struct-33804" status="VALID"><orgName>Université Lumière - Lyon 2</orgName><orgName type="acronym">UL2</orgName><desc><address><addrLine>86, rue Pasteur - 69007 Lyon</addrLine><country key="FR"></country></address><ref type="url">http://www.univ-lyon2.fr</ref></desc></org></tutelle><tutelle active="#struct-126765" type="direct"><org type="institution" xml:id="struct-126765" status="VALID"><orgName>École Centrale de Lyon</orgName><orgName type="acronym">ECL</orgName><desc><address><addrLine>36 avenue Guy de Collongue - 69134 Ecully cedex</addrLine><country key="FR"></country></address><ref type="url">http://www.ec-lyon.fr</ref></desc></org></tutelle><tutelle active="#struct-194495" type="direct"><org type="institution" xml:id="struct-194495" status="VALID"><orgName>Université Claude Bernard Lyon 1</orgName><orgName type="acronym">UCBL</orgName><desc><address><addrLine>43, boulevard du 11 novembre 1918, 69622 Villeurbanne cedex</addrLine><country key="FR"></country></address><ref type="url">http://www.univ-lyon1.fr/</ref></desc></org></tutelle><tutelle name="- LYON" active="#struct-301232" type="direct"><org type="institution" xml:id="struct-301232" status="VALID"><orgName>Institut National des Sciences Appliquées</orgName><orgName type="acronym">INSA</orgName><desc><address><country key="FR"></country></address></desc></org></tutelle><tutelle name="UMR5205" active="#struct-441569" type="direct"><org type="institution" xml:id="struct-441569" status="VALID"><idno type="ISNI">0000000122597504</idno><idno type="IdRef">02636817X</idno><orgName>Centre National de la Recherche Scientifique</orgName><orgName type="acronym">CNRS</orgName><date type="start">1939-10-19</date><desc><address><country key="FR"></country></address><ref type="url">http://www.cnrs.fr/</ref></desc></org></tutelle></tutelles></hal:affiliation><country>France</country><placeName><settlement type="city">Lyon</settlement><region type="region" nuts="2">Auvergne-Rhône-Alpes</region><region type="old region" nuts="2">Rhône-Alpes</region></placeName><orgName type="university">Université Claude Bernard Lyon1</orgName><orgName type="institution" wicri:auto="newGroup">Université de Lyon</orgName></affiliation></author><author><name sortKey="Desai, Jaydev" sort="Desai, Jaydev" uniqKey="Desai J" first="Jaydev" last="Desai">Jaydev Desai</name><affiliation wicri:level="1"><hal:affiliation type="laboratory" xml:id="struct-2003" status="VALID"><orgName>Laboratoire d'InfoRmatique en Image et Systèmes d'information</orgName><orgName type="acronym">LIRIS</orgName><desc><address><addrLine>Bâtiment Blaise Pascal - 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69134 Ecully cedex</addrLine><country key="FR"></country></address><ref type="url">http://www.ec-lyon.fr</ref></desc></org></tutelle><tutelle active="#struct-194495" type="direct"><org type="institution" xml:id="struct-194495" status="VALID"><orgName>Université Claude Bernard Lyon 1</orgName><orgName type="acronym">UCBL</orgName><desc><address><addrLine>43, boulevard du 11 novembre 1918, 69622 Villeurbanne cedex</addrLine><country key="FR"></country></address><ref type="url">http://www.univ-lyon1.fr/</ref></desc></org></tutelle><tutelle name="- LYON" active="#struct-301232" type="direct"><org type="institution" xml:id="struct-301232" status="VALID"><orgName>Institut National des Sciences Appliquées</orgName><orgName type="acronym">INSA</orgName><desc><address><country key="FR"></country></address></desc></org></tutelle><tutelle name="UMR5205" active="#struct-441569" type="direct"><org type="institution" xml:id="struct-441569" status="VALID"><idno type="ISNI">0000000122597504</idno><idno type="IdRef">02636817X</idno><orgName>Centre National de la Recherche Scientifique</orgName><orgName type="acronym">CNRS</orgName><date type="start">1939-10-19</date><desc><address><country key="FR"></country></address><ref type="url">http://www.cnrs.fr/</ref></desc></org></tutelle></tutelles></hal:affiliation><country>France</country><placeName><settlement type="city">Lyon</settlement><region type="region" nuts="2">Auvergne-Rhône-Alpes</region><region type="old region" nuts="2">Rhône-Alpes</region></placeName><orgName type="university">Université Claude Bernard Lyon1</orgName><orgName type="institution" wicri:auto="newGroup">Université de Lyon</orgName></affiliation></author></analytic><idno type="DOI">10.1109/IROS.2009.5354351</idno></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="mix" xml:lang="fr"><term>AFM</term><term>Cell indentation</term><term>FEM</term><term>Saint-Venant Kirchhoff</term><term>biomécanique</term><term>haptic feedback device</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">In order to understand and characterize the mechanical property and response of the mouse embryonic stem cells (mESC), we used an atomic force microscope (AFM) combined with a PHANToM haptic feedback device. Atomic force microscopy has rapidly become a valuable tool for quantifying the biophysical properties of single cells or a collection of cells through force measurements. We report herein the mechanical characterization of single mESC using indentation-relaxation measurements with micro-sphere AFM probes for fixed and live undifferentiated mESC. During cell indentation for both live and fixed undifferentiated cells, we provided force feedback to the user in real-time through the PHANToM haptic feedback device as the AFM tip was deforming the cell. The force was amplified for the human operator to perceive the change in force during cell indentation by the AFM cantilever. This information can be used as a mechanical marker to characterize state of the cell (live and fixed). As the interpretation of atomic force microscopy-based indentation tests is highly dependent on the use of an appropriate theoretical model of the testing configuration, various contact models are presented to predict the mechanical behavior of an individual mouse embryonic stem cells (mESC) in different states. A comparison study with finite element simulations (FEM) of spherical tip indentation demonstrates the effectiveness of our computational model to predict the mESC deformation during indentation and relaxation nanomanipulation tasks.</div></front></TEI><affiliations><list><country><li>France</li></country><region><li>Auvergne-Rhône-Alpes</li><li>Centre-Val de Loire</li><li>Rhône-Alpes</li><li>Région Centre</li></region><settlement><li>Lyon</li><li>Orléans</li></settlement><orgName><li>Centre Val de Loire Université</li><li>Université Claude Bernard Lyon1</li><li>Université d'Orléans</li><li>Université de Lyon</li></orgName></list><tree><country name="France"><region name="Région Centre"><name sortKey="Ladjal, Hamid" sort="Ladjal, Hamid" uniqKey="Ladjal H" first="Hamid" last="Ladjal">Hamid Ladjal</name></region><name sortKey="Desai, Jaydev" sort="Desai, Jaydev" uniqKey="Desai J" first="Jaydev" last="Desai">Jaydev Desai</name><name sortKey="Ferreira, A" sort="Ferreira, A" uniqKey="Ferreira A" first="A." last="Ferreira">A. Ferreira</name><name sortKey="Hanus, Jean Luc" sort="Hanus, Jean Luc" uniqKey="Hanus J" first="Jean-Luc" last="Hanus">Jean-Luc Hanus</name><name sortKey="Keefer, Carol" sort="Keefer, Carol" uniqKey="Keefer C" first="Carol" last="Keefer">Carol Keefer</name><name sortKey="Pillarisetti, Anand" sort="Pillarisetti, Anand" uniqKey="Pillarisetti A" first="Anand" last="Pillarisetti">Anand Pillarisetti</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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