Cross-linking Mass Spectrometry and Mutagenesis Confirm the Functional Importance of Surface Interactions between CYP3A4 and Holo/Apo Cytochrome b5
Identifieur interne : 000368 ( Ncbi/Curation ); précédent : 000367; suivant : 000369Cross-linking Mass Spectrometry and Mutagenesis Confirm the Functional Importance of Surface Interactions between CYP3A4 and Holo/Apo Cytochrome b5
Auteurs : Chunsheng Zhao [États-Unis] ; Qiuxia Gao [États-Unis] ; Arthur G. Roberts [États-Unis] ; Scott A. Shaffer [États-Unis] ; Catalin E. Doneanu [États-Unis] ; Song Xue [États-Unis] ; David R. Goodlett [États-Unis] ; Sidney D. Nelson [États-Unis] ; William M. Atkins [États-Unis]Source :
- Biochemistry [ 0006-2960 ] ; 2012.
Abstract
Cytochrome
Url:
DOI: 10.1021/bi301069r
PubMed: 23150942
PubMed Central: 3568533
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</italic>
</title>
<author><name sortKey="Zhao, Chunsheng" sort="Zhao, Chunsheng" uniqKey="Zhao C" first="Chunsheng" last="Zhao">Chunsheng Zhao</name>
<affiliation wicri:level="2"><nlm:aff id="A1">Department of Medicinal Chemistry, University of Washington, Box 357610, Seattle, Washington 98195</nlm:aff>
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<placeName><region type="state">Washington (État)</region>
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<author><name sortKey="Gao, Qiuxia" sort="Gao, Qiuxia" uniqKey="Gao Q" first="Qiuxia" last="Gao">Qiuxia Gao</name>
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<wicri:cityArea>Department of Medicinal Chemistry, University of Washington, Box 357610, Seattle</wicri:cityArea>
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<author><name sortKey="Xue, Song" sort="Xue, Song" uniqKey="Xue S" first="Song" last="Xue">Song Xue</name>
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<author><name sortKey="Goodlett, David R" sort="Goodlett, David R" uniqKey="Goodlett D" first="David R." last="Goodlett">David R. Goodlett</name>
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<author><name sortKey="Atkins, William M" sort="Atkins, William M" uniqKey="Atkins W" first="William M." last="Atkins">William M. Atkins</name>
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<wicri:cityArea>Department of Medicinal Chemistry, University of Washington, Box 357610, Seattle</wicri:cityArea>
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<sourceDesc><biblStruct><analytic><title xml:lang="en" level="a" type="main">Cross-linking Mass Spectrometry and Mutagenesis Confirm the Functional Importance of Surface Interactions between CYP3A4 and Holo/Apo Cytochrome <italic>b<sub>5</sub>
</italic>
</title>
<author><name sortKey="Zhao, Chunsheng" sort="Zhao, Chunsheng" uniqKey="Zhao C" first="Chunsheng" last="Zhao">Chunsheng Zhao</name>
<affiliation wicri:level="2"><nlm:aff id="A1">Department of Medicinal Chemistry, University of Washington, Box 357610, Seattle, Washington 98195</nlm:aff>
<country xml:lang="fr">États-Unis</country>
<placeName><region type="state">Washington (État)</region>
</placeName>
<wicri:cityArea>Department of Medicinal Chemistry, University of Washington, Box 357610, Seattle</wicri:cityArea>
</affiliation>
</author>
<author><name sortKey="Gao, Qiuxia" sort="Gao, Qiuxia" uniqKey="Gao Q" first="Qiuxia" last="Gao">Qiuxia Gao</name>
<affiliation wicri:level="2"><nlm:aff id="A1">Department of Medicinal Chemistry, University of Washington, Box 357610, Seattle, Washington 98195</nlm:aff>
<country xml:lang="fr">États-Unis</country>
<placeName><region type="state">Washington (État)</region>
</placeName>
<wicri:cityArea>Department of Medicinal Chemistry, University of Washington, Box 357610, Seattle</wicri:cityArea>
</affiliation>
</author>
<author><name sortKey="Roberts, Arthur G" sort="Roberts, Arthur G" uniqKey="Roberts A" first="Arthur G." last="Roberts">Arthur G. Roberts</name>
<affiliation wicri:level="2"><nlm:aff id="A3">Pharmaceutical and Biomedical Sciences, University of Georgia, Athens, GA 30605</nlm:aff>
<country xml:lang="fr">États-Unis</country>
<placeName><region type="state">Géorgie (États-Unis)</region>
</placeName>
<wicri:cityArea>Pharmaceutical and Biomedical Sciences, University of Georgia, Athens</wicri:cityArea>
</affiliation>
</author>
<author><name sortKey="Shaffer, Scott A" sort="Shaffer, Scott A" uniqKey="Shaffer S" first="Scott A." last="Shaffer">Scott A. Shaffer</name>
<affiliation wicri:level="2"><nlm:aff id="A2">Biochemistry and Molecular Pharmacology, University of Massachusetts Medical School, Shrewsbury, MA 01545</nlm:aff>
<country xml:lang="fr">États-Unis</country>
<placeName><region type="state">Massachusetts</region>
</placeName>
<wicri:cityArea>Biochemistry and Molecular Pharmacology, University of Massachusetts Medical School, Shrewsbury</wicri:cityArea>
</affiliation>
</author>
<author><name sortKey="Doneanu, Catalin E" sort="Doneanu, Catalin E" uniqKey="Doneanu C" first="Catalin E." last="Doneanu">Catalin E. Doneanu</name>
<affiliation wicri:level="2"><nlm:aff id="A1">Department of Medicinal Chemistry, University of Washington, Box 357610, Seattle, Washington 98195</nlm:aff>
<country xml:lang="fr">États-Unis</country>
<placeName><region type="state">Washington (État)</region>
</placeName>
<wicri:cityArea>Department of Medicinal Chemistry, University of Washington, Box 357610, Seattle</wicri:cityArea>
</affiliation>
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<author><name sortKey="Xue, Song" sort="Xue, Song" uniqKey="Xue S" first="Song" last="Xue">Song Xue</name>
<affiliation wicri:level="2"><nlm:aff id="A4">Microsoft Incorporation, 1 Microsoft Way, Redmond, Washington 98052</nlm:aff>
<country xml:lang="fr">États-Unis</country>
<placeName><region type="state">Washington (État)</region>
</placeName>
<wicri:cityArea>Microsoft Incorporation, 1 Microsoft Way, Redmond</wicri:cityArea>
</affiliation>
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<author><name sortKey="Goodlett, David R" sort="Goodlett, David R" uniqKey="Goodlett D" first="David R." last="Goodlett">David R. Goodlett</name>
<affiliation wicri:level="2"><nlm:aff id="A1">Department of Medicinal Chemistry, University of Washington, Box 357610, Seattle, Washington 98195</nlm:aff>
<country xml:lang="fr">États-Unis</country>
<placeName><region type="state">Washington (État)</region>
</placeName>
<wicri:cityArea>Department of Medicinal Chemistry, University of Washington, Box 357610, Seattle</wicri:cityArea>
</affiliation>
</author>
<author><name sortKey="Nelson, Sidney D" sort="Nelson, Sidney D" uniqKey="Nelson S" first="Sidney D." last="Nelson">Sidney D. Nelson</name>
<affiliation wicri:level="2"><nlm:aff id="A1">Department of Medicinal Chemistry, University of Washington, Box 357610, Seattle, Washington 98195</nlm:aff>
<country xml:lang="fr">États-Unis</country>
<placeName><region type="state">Washington (État)</region>
</placeName>
<wicri:cityArea>Department of Medicinal Chemistry, University of Washington, Box 357610, Seattle</wicri:cityArea>
</affiliation>
</author>
<author><name sortKey="Atkins, William M" sort="Atkins, William M" uniqKey="Atkins W" first="William M." last="Atkins">William M. Atkins</name>
<affiliation wicri:level="2"><nlm:aff id="A1">Department of Medicinal Chemistry, University of Washington, Box 357610, Seattle, Washington 98195</nlm:aff>
<country xml:lang="fr">États-Unis</country>
<placeName><region type="state">Washington (État)</region>
</placeName>
<wicri:cityArea>Department of Medicinal Chemistry, University of Washington, Box 357610, Seattle</wicri:cityArea>
</affiliation>
</author>
</analytic>
<series><title level="j">Biochemistry</title>
<idno type="ISSN">0006-2960</idno>
<idno type="eISSN">1520-4995</idno>
<imprint><date when="2012">2012</date>
</imprint>
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<front><div type="abstract" xml:lang="en"><p id="P1">Cytochrome <italic>b<sub>5</sub>
</italic>
(cyt <italic>b<sub>5</sub>
</italic>
) is one of the key components in the microsomal cytochrome P450 monooxygenase system. Consensus has not been reached on the underlying mechanism of cyt <italic>b<sub>5</sub>
</italic>
modulation of CYP catalysis. Both cyt <italic>b<sub>5</sub>
</italic>
and apo <italic>b<sub>5</sub>
</italic>
, are reported to stimulate the activity of several P450 isoforms. In the present study, the surface interactions of both holo and apo <italic>b<sub>5</sub>
</italic>
with CYP3A4 were investigated and compared for the first time. Chemical cross-linking coupled with mass spectrometric analysis was used to identify the potential electrostatic interactions between the protein surfaces. Subsequently, the interaction models of holo/apo <italic>b<sub>5</sub>
</italic>
with CYP3A4 were built using the identified interacting sites as constraints. Both cyt <italic>b<sub>5</sub>
</italic>
and apo <italic>b<sub>5</sub>
</italic>
were predicted to bind to the same groove on CYP3A4 with close contacts to the B-B’ loop of CYP3A4, a substrate recognition site (SRS). Mutagenesis studies further confirmed that the interacting sites on CYP3A4 (Lys96, Lys127 and Lys421) are of functional importance. Mutation of these residues reduced or abolished cyt <italic>b<sub>5</sub>
</italic>
binding affinity. The critical role of Arg446 on CYP3A4 in binding to cyt <italic>b<sub>5</sub>
</italic>
and/or cytochrome P450 reductase (CPR) was also discovered. The results indicated that electrostatic interactions on the interface of the two proteins are functionally important. The results indicate that the apo cyt <italic>b</italic>
<sub>5</sub>
can dock with CYP3A4 in a manner analogous to holo cyt <italic>b</italic>
<sub>5</sub>
so electron transfer from cyt <italic>b</italic>
<sub>5</sub>
is not required for its effects.</p>
</div>
</front>
</TEI>
</record>
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