Discovery of cyanophage genomes which contain mitochondrial DNA polymerase.
Identifieur interne : 000703 ( Main/Merge ); précédent : 000702; suivant : 000704Discovery of cyanophage genomes which contain mitochondrial DNA polymerase.
Auteurs : Yi-Wah Chan [Royaume-Uni] ; Remus Mohr ; Andrew D. Millard ; Antony B. Holmes ; Anthony W. Larkum ; Anna L. Whitworth ; Nicholas H. Mann ; David J. Scanlan ; Wolfgang R. Hess ; Martha R J. ClokieSource :
- Molecular biology and evolution [ 1537-1719 ] ; 2011.
English descriptors
- KwdEn :
- Bacteriophages (classification), Bacteriophages (enzymology), Bacteriophages (genetics), Cyanobacteria (virology), DNA, Mitochondrial (genetics), DNA-Directed DNA Polymerase (genetics), DNA-Directed DNA Polymerase (metabolism), Evolution, Molecular, Gene Expression Regulation, Viral, Genome, Viral, Phylogeny.
- MESH :
- chemical , genetics : DNA, Mitochondrial, DNA-Directed DNA Polymerase.
- classification : Bacteriophages.
- enzymology : Bacteriophages.
- genetics : Bacteriophages.
- chemical , metabolism : DNA-Directed DNA Polymerase.
- virology : Cyanobacteria.
- Evolution, Molecular, Gene Expression Regulation, Viral, Genome, Viral, Phylogeny.
Abstract
DNA polymerase γ is a family A DNA polymerase responsible for the replication of mitochondrial DNA in eukaryotes. The origins of DNA polymerase γ have remained elusive because it is not present in any known bacterium, though it has been hypothesized that mitochondria may have inherited the enzyme by phage-mediated nonorthologous displacement. Here, we present an analysis of two full-length homologues of this gene, which were found in the genomes of two bacteriophages, which infect the chlorophyll-d containing cyanobacterium Acaryochloris marina. Phylogenetic analyses of these phage DNA polymerase γ proteins show that they branch deeply within the DNA polymerase γ clade and therefore share a common origin with their eukaryotic homologues. We also found homologues of these phage polymerases in the environmental Community Cyberinfrastructure for Advanced Microbial Ecology Research and Analysis (CAMERA) database, which fell in the same clade. An analysis of the CAMERA assemblies containing the environmental homologues together with the filter fraction metadata indicated some of these assemblies may be of bacterial origin. We also show that the phage-encoded DNA polymerase γ is highly transcribed as the phage genomes are replicated. These findings provide data that may assist in reconstructing the evolution of mitochondria.
DOI: 10.1093/molbev/msr041
PubMed: 21335340
Links toward previous steps (curation, corpus...)
- to stream PubMed, to step Corpus: 000037
- to stream PubMed, to step Curation: 000037
- to stream PubMed, to step Checkpoint: 000037
- to stream Ncbi, to step Merge: 000198
- to stream Ncbi, to step Curation: 000198
- to stream Ncbi, to step Checkpoint: 000198
Links to Exploration step
pubmed:21335340Le document en format XML
<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">Discovery of cyanophage genomes which contain mitochondrial DNA polymerase.</title><author><name sortKey="Chan, Yi Wah" sort="Chan, Yi Wah" uniqKey="Chan Y" first="Yi-Wah" last="Chan">Yi-Wah Chan</name><affiliation wicri:level="1"><nlm:affiliation>Reproductive Health, Clinical Sciences Research Institute, University Hospital, Coventry, UK.</nlm:affiliation><country xml:lang="fr">Royaume-Uni</country><wicri:regionArea>Reproductive Health, Clinical Sciences Research Institute, University Hospital, Coventry</wicri:regionArea><wicri:noRegion>Coventry</wicri:noRegion></affiliation></author><author><name sortKey="Mohr, Remus" sort="Mohr, Remus" uniqKey="Mohr R" first="Remus" last="Mohr">Remus Mohr</name></author><author><name sortKey="Millard, Andrew D" sort="Millard, Andrew D" uniqKey="Millard A" first="Andrew D" last="Millard">Andrew D. Millard</name></author><author><name sortKey="Holmes, Antony B" sort="Holmes, Antony B" uniqKey="Holmes A" first="Antony B" last="Holmes">Antony B. Holmes</name></author><author><name sortKey="Larkum, Anthony W" sort="Larkum, Anthony W" uniqKey="Larkum A" first="Anthony W" last="Larkum">Anthony W. Larkum</name></author><author><name sortKey="Whitworth, Anna L" sort="Whitworth, Anna L" uniqKey="Whitworth A" first="Anna L" last="Whitworth">Anna L. Whitworth</name></author><author><name sortKey="Mann, Nicholas H" sort="Mann, Nicholas H" uniqKey="Mann N" first="Nicholas H" last="Mann">Nicholas H. Mann</name></author><author><name sortKey="Scanlan, David J" sort="Scanlan, David J" uniqKey="Scanlan D" first="David J" last="Scanlan">David J. Scanlan</name></author><author><name sortKey="Hess, Wolfgang R" sort="Hess, Wolfgang R" uniqKey="Hess W" first="Wolfgang R" last="Hess">Wolfgang R. Hess</name></author><author><name sortKey="Clokie, Martha R J" sort="Clokie, Martha R J" uniqKey="Clokie M" first="Martha R J" last="Clokie">Martha R J. Clokie</name></author></titleStmt><publicationStmt><idno type="wicri:source">PubMed</idno><date when="2011">2011</date><idno type="doi">10.1093/molbev/msr041</idno><idno type="RBID">pubmed:21335340</idno><idno type="pmid">21335340</idno><idno type="wicri:Area/PubMed/Corpus">000037</idno><idno type="wicri:Area/PubMed/Curation">000037</idno><idno type="wicri:Area/PubMed/Checkpoint">000037</idno><idno type="wicri:Area/Ncbi/Merge">000198</idno><idno type="wicri:Area/Ncbi/Curation">000198</idno><idno type="wicri:Area/Ncbi/Checkpoint">000198</idno><idno type="wicri:Area/Main/Merge">000703</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en">Discovery of cyanophage genomes which contain mitochondrial DNA polymerase.</title><author><name sortKey="Chan, Yi Wah" sort="Chan, Yi Wah" uniqKey="Chan Y" first="Yi-Wah" last="Chan">Yi-Wah Chan</name><affiliation wicri:level="1"><nlm:affiliation>Reproductive Health, Clinical Sciences Research Institute, University Hospital, Coventry, UK.</nlm:affiliation><country xml:lang="fr">Royaume-Uni</country><wicri:regionArea>Reproductive Health, Clinical Sciences Research Institute, University Hospital, Coventry</wicri:regionArea><wicri:noRegion>Coventry</wicri:noRegion></affiliation></author><author><name sortKey="Mohr, Remus" sort="Mohr, Remus" uniqKey="Mohr R" first="Remus" last="Mohr">Remus Mohr</name></author><author><name sortKey="Millard, Andrew D" sort="Millard, Andrew D" uniqKey="Millard A" first="Andrew D" last="Millard">Andrew D. Millard</name></author><author><name sortKey="Holmes, Antony B" sort="Holmes, Antony B" uniqKey="Holmes A" first="Antony B" last="Holmes">Antony B. Holmes</name></author><author><name sortKey="Larkum, Anthony W" sort="Larkum, Anthony W" uniqKey="Larkum A" first="Anthony W" last="Larkum">Anthony W. Larkum</name></author><author><name sortKey="Whitworth, Anna L" sort="Whitworth, Anna L" uniqKey="Whitworth A" first="Anna L" last="Whitworth">Anna L. Whitworth</name></author><author><name sortKey="Mann, Nicholas H" sort="Mann, Nicholas H" uniqKey="Mann N" first="Nicholas H" last="Mann">Nicholas H. Mann</name></author><author><name sortKey="Scanlan, David J" sort="Scanlan, David J" uniqKey="Scanlan D" first="David J" last="Scanlan">David J. Scanlan</name></author><author><name sortKey="Hess, Wolfgang R" sort="Hess, Wolfgang R" uniqKey="Hess W" first="Wolfgang R" last="Hess">Wolfgang R. Hess</name></author><author><name sortKey="Clokie, Martha R J" sort="Clokie, Martha R J" uniqKey="Clokie M" first="Martha R J" last="Clokie">Martha R J. Clokie</name></author></analytic><series><title level="j">Molecular biology and evolution</title><idno type="eISSN">1537-1719</idno><imprint><date when="2011" type="published">2011</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Bacteriophages (classification)</term><term>Bacteriophages (enzymology)</term><term>Bacteriophages (genetics)</term><term>Cyanobacteria (virology)</term><term>DNA, Mitochondrial (genetics)</term><term>DNA-Directed DNA Polymerase (genetics)</term><term>DNA-Directed DNA Polymerase (metabolism)</term><term>Evolution, Molecular</term><term>Gene Expression Regulation, Viral</term><term>Genome, Viral</term><term>Phylogeny</term></keywords><keywords scheme="MESH" type="chemical" qualifier="genetics" xml:lang="en"><term>DNA, Mitochondrial</term><term>DNA-Directed DNA Polymerase</term></keywords><keywords scheme="MESH" qualifier="classification" xml:lang="en"><term>Bacteriophages</term></keywords><keywords scheme="MESH" qualifier="enzymology" xml:lang="en"><term>Bacteriophages</term></keywords><keywords scheme="MESH" qualifier="genetics" xml:lang="en"><term>Bacteriophages</term></keywords><keywords scheme="MESH" type="chemical" qualifier="metabolism" xml:lang="en"><term>DNA-Directed DNA Polymerase</term></keywords><keywords scheme="MESH" qualifier="virology" xml:lang="en"><term>Cyanobacteria</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Evolution, Molecular</term><term>Gene Expression Regulation, Viral</term><term>Genome, Viral</term><term>Phylogeny</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">DNA polymerase γ is a family A DNA polymerase responsible for the replication of mitochondrial DNA in eukaryotes. The origins of DNA polymerase γ have remained elusive because it is not present in any known bacterium, though it has been hypothesized that mitochondria may have inherited the enzyme by phage-mediated nonorthologous displacement. Here, we present an analysis of two full-length homologues of this gene, which were found in the genomes of two bacteriophages, which infect the chlorophyll-d containing cyanobacterium Acaryochloris marina. Phylogenetic analyses of these phage DNA polymerase γ proteins show that they branch deeply within the DNA polymerase γ clade and therefore share a common origin with their eukaryotic homologues. We also found homologues of these phage polymerases in the environmental Community Cyberinfrastructure for Advanced Microbial Ecology Research and Analysis (CAMERA) database, which fell in the same clade. An analysis of the CAMERA assemblies containing the environmental homologues together with the filter fraction metadata indicated some of these assemblies may be of bacterial origin. We also show that the phage-encoded DNA polymerase γ is highly transcribed as the phage genomes are replicated. These findings provide data that may assist in reconstructing the evolution of mitochondria.</div></front></TEI></record>Pour manipuler ce document sous Unix (Dilib)
EXPLOR_STEP=$WICRI_ROOT/Ticri/CIDE/explor/CyberinfraV1/Data/Main/Merge
HfdSelect -h $EXPLOR_STEP/biblio.hfd -nk 000703 | SxmlIndent | more
Ou
HfdSelect -h $EXPLOR_AREA/Data/Main/Merge/biblio.hfd -nk 000703 | SxmlIndent | more
Pour mettre un lien sur cette page dans le réseau Wicri
{{Explor lien
|wiki= Ticri/CIDE
|area= CyberinfraV1
|flux= Main
|étape= Merge
|type= RBID
|clé= pubmed:21335340
|texte= Discovery of cyanophage genomes which contain mitochondrial DNA polymerase.
}}
Pour générer des pages wiki
HfdIndexSelect -h $EXPLOR_AREA/Data/Main/Merge/RBID.i -Sk "pubmed:21335340" \
| HfdSelect -Kh $EXPLOR_AREA/Data/Main/Merge/biblio.hfd \
| NlmPubMed2Wicri -a CyberinfraV1
| This area was generated with Dilib version V0.6.25. |  |