Transport by Populations of Fast and Slow Kinesins Uncovers Novel Family-Dependent Motor Characteristics Important for In Vivo Function
Identifieur interne : 000166 ( Main/Exploration ); précédent : 000165; suivant : 000167Transport by Populations of Fast and Slow Kinesins Uncovers Novel Family-Dependent Motor Characteristics Important for In Vivo Function
Auteurs : Göker Arpa [États-Unis] ; Shankar Shastry [États-Unis] ; William O. Hancock [États-Unis] ; Erkan Tüzel [États-Unis]Source :
- Biophysical Journal [ 0006-3495 ] ; 2014.
Abstract
Intracellular cargo transport frequently involves multiple motor types, either having opposite directionality or having the same directionality but different speeds. Although significant progress has been made in characterizing kinesin motors at the single-molecule level, predicting their ensemble behavior is challenging and requires tight coupling between experiments and modeling to uncover the underlying motor behavior. To understand how diverse kinesins attached to the same cargo coordinate their movement, we carried out microtubule gliding assays using pairwise mixtures of motors from the kinesin-1, -2, -3, -5, and -7 families engineered to have identical run lengths and surface attachments. Uniform motor densities were used and microtubule gliding speeds were measured for varying proportions of fast and slow motors. A coarse-grained computational model of gliding assays was developed and found to recapitulate the experiments. Simulations incorporated published force-dependent velocities and run lengths, along with mechanical interactions between motors bound to the same microtubule. The simulations show that the force-dependence of detachment is the key parameter that determines gliding speed in multimotor assays, while motor compliance, surface density, and stall force all play minimal roles. Simulations also provide estimates for force-dependent dissociation rates, suggesting that kinesin-1 and the mitotic motors kinesin-5 and -7 maintain microtubule association against loads, whereas kinesin-2 and -3 readily detach. This work uncovers unexpected motor behavior in multimotor ensembles and clarifies functional differences between kinesins that carry out distinct mechanical tasks in cells.
Url:
DOI: 10.1016/j.bpj.2014.09.009
PubMed: 25418170
PubMed Central: 4213720
Affiliations:
Links toward previous steps (curation, corpus...)
- to stream Pmc, to step Corpus: 000357
- to stream Pmc, to step Curation: 000357
- to stream Pmc, to step Checkpoint: 000156
- to stream Ncbi, to step Merge: 000583
- to stream Ncbi, to step Curation: 000583
- to stream Ncbi, to step Checkpoint: 000583
- to stream Main, to step Merge: 000166
- to stream Main, to step Curation: 000166
Le document en format XML
<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">Transport by Populations of Fast and Slow Kinesins Uncovers Novel Family-Dependent Motor Characteristics Important for In Vivo Function</title>
<author><name sortKey="Arpa, Goker" sort="Arpa, Goker" uniqKey="Arpa G" first="Göker" last="Arpa">Göker Arpa</name>
<affiliation wicri:level="2"><nlm:aff id="aff1">Department of Physics, Worcester Polytechnic Institute, Worcester, Massachusetts</nlm:aff>
<country xml:lang="fr">États-Unis</country>
<placeName><region type="state">Massachusetts</region>
</placeName>
<wicri:cityArea>Department of Physics, Worcester Polytechnic Institute, Worcester</wicri:cityArea>
</affiliation>
</author>
<author><name sortKey="Shastry, Shankar" sort="Shastry, Shankar" uniqKey="Shastry S" first="Shankar" last="Shastry">Shankar Shastry</name>
<affiliation wicri:level="2"><nlm:aff id="aff2">Department of Biomedical Engineering, The Pennsylvania State University, University Park, Pennsylvania</nlm:aff>
<country xml:lang="fr">États-Unis</country>
<placeName><region type="state">Pennsylvanie</region>
</placeName>
<wicri:cityArea>Department of Biomedical Engineering, The Pennsylvania State University, University Park</wicri:cityArea>
</affiliation>
</author>
<author><name sortKey="Hancock, William O" sort="Hancock, William O" uniqKey="Hancock W" first="William O." last="Hancock">William O. Hancock</name>
<affiliation wicri:level="2"><nlm:aff id="aff2">Department of Biomedical Engineering, The Pennsylvania State University, University Park, Pennsylvania</nlm:aff>
<country xml:lang="fr">États-Unis</country>
<placeName><region type="state">Pennsylvanie</region>
</placeName>
<wicri:cityArea>Department of Biomedical Engineering, The Pennsylvania State University, University Park</wicri:cityArea>
</affiliation>
</author>
<author><name sortKey="Tuzel, Erkan" sort="Tuzel, Erkan" uniqKey="Tuzel E" first="Erkan" last="Tüzel">Erkan Tüzel</name>
<affiliation wicri:level="2"><nlm:aff id="aff1">Department of Physics, Worcester Polytechnic Institute, Worcester, Massachusetts</nlm:aff>
<country xml:lang="fr">États-Unis</country>
<placeName><region type="state">Massachusetts</region>
</placeName>
<wicri:cityArea>Department of Physics, Worcester Polytechnic Institute, Worcester</wicri:cityArea>
</affiliation>
</author>
</titleStmt>
<publicationStmt><idno type="wicri:source">PMC</idno>
<idno type="pmid">25418170</idno>
<idno type="pmc">4213720</idno>
<idno type="url">http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4213720</idno>
<idno type="RBID">PMC:4213720</idno>
<idno type="doi">10.1016/j.bpj.2014.09.009</idno>
<date when="2014">2014</date>
<idno type="wicri:Area/Pmc/Corpus">000357</idno>
<idno type="wicri:Area/Pmc/Curation">000357</idno>
<idno type="wicri:Area/Pmc/Checkpoint">000156</idno>
<idno type="wicri:Area/Ncbi/Merge">000583</idno>
<idno type="wicri:Area/Ncbi/Curation">000583</idno>
<idno type="wicri:Area/Ncbi/Checkpoint">000583</idno>
<idno type="wicri:doubleKey">0006-3495:2014:Arpa G:transport:by:populations</idno>
<idno type="wicri:Area/Main/Merge">000166</idno>
<idno type="wicri:Area/Main/Curation">000166</idno>
<idno type="wicri:Area/Main/Exploration">000166</idno>
</publicationStmt>
<sourceDesc><biblStruct><analytic><title xml:lang="en" level="a" type="main">Transport by Populations of Fast and Slow Kinesins Uncovers Novel Family-Dependent Motor Characteristics Important for In Vivo Function</title>
<author><name sortKey="Arpa, Goker" sort="Arpa, Goker" uniqKey="Arpa G" first="Göker" last="Arpa">Göker Arpa</name>
<affiliation wicri:level="2"><nlm:aff id="aff1">Department of Physics, Worcester Polytechnic Institute, Worcester, Massachusetts</nlm:aff>
<country xml:lang="fr">États-Unis</country>
<placeName><region type="state">Massachusetts</region>
</placeName>
<wicri:cityArea>Department of Physics, Worcester Polytechnic Institute, Worcester</wicri:cityArea>
</affiliation>
</author>
<author><name sortKey="Shastry, Shankar" sort="Shastry, Shankar" uniqKey="Shastry S" first="Shankar" last="Shastry">Shankar Shastry</name>
<affiliation wicri:level="2"><nlm:aff id="aff2">Department of Biomedical Engineering, The Pennsylvania State University, University Park, Pennsylvania</nlm:aff>
<country xml:lang="fr">États-Unis</country>
<placeName><region type="state">Pennsylvanie</region>
</placeName>
<wicri:cityArea>Department of Biomedical Engineering, The Pennsylvania State University, University Park</wicri:cityArea>
</affiliation>
</author>
<author><name sortKey="Hancock, William O" sort="Hancock, William O" uniqKey="Hancock W" first="William O." last="Hancock">William O. Hancock</name>
<affiliation wicri:level="2"><nlm:aff id="aff2">Department of Biomedical Engineering, The Pennsylvania State University, University Park, Pennsylvania</nlm:aff>
<country xml:lang="fr">États-Unis</country>
<placeName><region type="state">Pennsylvanie</region>
</placeName>
<wicri:cityArea>Department of Biomedical Engineering, The Pennsylvania State University, University Park</wicri:cityArea>
</affiliation>
</author>
<author><name sortKey="Tuzel, Erkan" sort="Tuzel, Erkan" uniqKey="Tuzel E" first="Erkan" last="Tüzel">Erkan Tüzel</name>
<affiliation wicri:level="2"><nlm:aff id="aff1">Department of Physics, Worcester Polytechnic Institute, Worcester, Massachusetts</nlm:aff>
<country xml:lang="fr">États-Unis</country>
<placeName><region type="state">Massachusetts</region>
</placeName>
<wicri:cityArea>Department of Physics, Worcester Polytechnic Institute, Worcester</wicri:cityArea>
</affiliation>
</author>
</analytic>
<series><title level="j">Biophysical Journal</title>
<idno type="ISSN">0006-3495</idno>
<idno type="eISSN">1542-0086</idno>
<imprint><date when="2014">2014</date>
</imprint>
</series>
</biblStruct>
</sourceDesc>
</fileDesc>
<profileDesc><textClass></textClass>
</profileDesc>
</teiHeader>
<front><div type="abstract" xml:lang="en"><p>Intracellular cargo transport frequently involves multiple motor types, either having opposite directionality or having the same directionality but different speeds. Although significant progress has been made in characterizing kinesin motors at the single-molecule level, predicting their ensemble behavior is challenging and requires tight coupling between experiments and modeling to uncover the underlying motor behavior. To understand how diverse kinesins attached to the same cargo coordinate their movement, we carried out microtubule gliding assays using pairwise mixtures of motors from the kinesin-1, -2, -3, -5, and -7 families engineered to have identical run lengths and surface attachments. Uniform motor densities were used and microtubule gliding speeds were measured for varying proportions of fast and slow motors. A coarse-grained computational model of gliding assays was developed and found to recapitulate the experiments. Simulations incorporated published force-dependent velocities and run lengths, along with mechanical interactions between motors bound to the same microtubule. The simulations show that the force-dependence of detachment is the key parameter that determines gliding speed in multimotor assays, while motor compliance, surface density, and stall force all play minimal roles. Simulations also provide estimates for force-dependent dissociation rates, suggesting that kinesin-1 and the mitotic motors kinesin-5 and -7 maintain microtubule association against loads, whereas kinesin-2 and -3 readily detach. This work uncovers unexpected motor behavior in multimotor ensembles and clarifies functional differences between kinesins that carry out distinct mechanical tasks in cells.</p>
</div>
</front>
</TEI>
<affiliations><list><country><li>États-Unis</li>
</country>
<region><li>Massachusetts</li>
<li>Pennsylvanie</li>
</region>
</list>
<tree><country name="États-Unis"><region name="Massachusetts"><name sortKey="Arpa, Goker" sort="Arpa, Goker" uniqKey="Arpa G" first="Göker" last="Arpa">Göker Arpa</name>
</region>
<name sortKey="Hancock, William O" sort="Hancock, William O" uniqKey="Hancock W" first="William O." last="Hancock">William O. Hancock</name>
<name sortKey="Shastry, Shankar" sort="Shastry, Shankar" uniqKey="Shastry S" first="Shankar" last="Shastry">Shankar Shastry</name>
<name sortKey="Tuzel, Erkan" sort="Tuzel, Erkan" uniqKey="Tuzel E" first="Erkan" last="Tüzel">Erkan Tüzel</name>
</country>
</tree>
</affiliations>
</record>
Pour manipuler ce document sous Unix (Dilib)
EXPLOR_STEP=$WICRI_ROOT/Ticri/CIDE/explor/CyberinfraV1/Data/Main/Exploration
HfdSelect -h $EXPLOR_STEP/biblio.hfd -nk 000166 | SxmlIndent | more
Ou
HfdSelect -h $EXPLOR_AREA/Data/Main/Exploration/biblio.hfd -nk 000166 | SxmlIndent | more
Pour mettre un lien sur cette page dans le réseau Wicri
{{Explor lien |wiki= Ticri/CIDE |area= CyberinfraV1 |flux= Main |étape= Exploration |type= RBID |clé= PMC:4213720 |texte= Transport by Populations of Fast and Slow Kinesins Uncovers Novel Family-Dependent Motor Characteristics Important for In Vivo Function }}
Pour générer des pages wiki
HfdIndexSelect -h $EXPLOR_AREA/Data/Main/Exploration/RBID.i -Sk "pubmed:25418170" \ | HfdSelect -Kh $EXPLOR_AREA/Data/Main/Exploration/biblio.hfd \ | NlmPubMed2Wicri -a CyberinfraV1
![]() | This area was generated with Dilib version V0.6.25. | ![]() |