Labeling of adenovirus particles with PARACEST agents.
Identifieur interne : 000597 ( PubMed/Corpus ); précédent : 000596; suivant : 000598Labeling of adenovirus particles with PARACEST agents.
Auteurs : Olga Vasalatiy ; Robert D. Gerard ; Piyu Zhao ; Xiankai Sun ; A Dean SherrySource :
- Bioconjugate chemistry [ 1043-1802 ] ; 2008.
English descriptors
- KwdEn :
- Adenoviridae (chemistry), Adenoviridae (genetics), Adenoviridae Infections (genetics), Adenoviridae Infections (virology), Cell Line, Chelating Agents (chemistry), Contrast Media (chemistry), Crown Compounds (chemistry), Humans, Indicators and Reagents, Kinetics, Ligands, Luciferases (genetics), Luminescence, Lutetium, Magnetic Resonance Spectroscopy, Metals (chemistry), Radioisotopes, Spectrometry, Mass, Electrospray Ionization, Thulium.
- MESH :
- chemical , chemistry : Chelating Agents, Contrast Media, Crown Compounds, Metals.
- chemistry : Adenoviridae.
- genetics : Adenoviridae, Adenoviridae Infections, Luciferases.
- virology : Adenoviridae Infections.
- Cell Line, Humans, Indicators and Reagents, Kinetics, Ligands, Luminescence, Lutetium, Magnetic Resonance Spectroscopy, Radioisotopes, Spectrometry, Mass, Electrospray Ionization, Thulium.
Abstract
Recombinant adenovirus type 5 particles (AdCMVLuc) were labeled with two different bifunctional ligands capable of forming stable complexes with paramagnetic lanthanide ions. The number of covalently attached ligands varied between 630 and 1960 per adenovirus particle depending upon the chemical reactivity of the bifunctional ligand (NHS ester versus isothiocyanide), the amount of excess ligand added, and the reaction time. The bioactivity of each labeled adenovirus derivative, as measured by the ability of the virus to infect cells and express luciferase, was shown to be highly dependent upon the number of covalently attached ligands. This indicates that certain amino groups, likely on the surface of the adenovirus fiber protein where cell binding is known to occur, are critical for viral attachment and infection. Addition of (177)Lu3+ to chemically modified versus control viruses demonstrated a significant amount of nonspecific binding of (177)Lu3+ to the virus particles that could not be sequestered by addition of excess DTPA. Thus, it became necessary to implement a prelabeling strategy for conjugation of preformed lanthanide ligand chelates to adenovirus particles. Using preformed Tm3+- L2, a large number of chelates having chemical exchange saturation transfer (CEST) properties were attached to the surface residues of AdCMVLuc without nonspecific binding of metal ions elsewhere on the virus particle. The potential of such conjugates to act as PARACEST imaging agents was tested using an on-resonance WALTZ sequence for CEST activation. A 12% decrease in bulk water signal intensity was observed relative to controls. This demonstrates that viral particles labeled with PARACEST-type imaging agents can potentially serve as targeted agents for molecular imaging.
DOI: 10.1021/bc7002605
PubMed: 18254605
Links to Exploration step
pubmed:18254605Le document en format XML
<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">Labeling of adenovirus particles with PARACEST agents.</title><author><name sortKey="Vasalatiy, Olga" sort="Vasalatiy, Olga" uniqKey="Vasalatiy O" first="Olga" last="Vasalatiy">Olga Vasalatiy</name><affiliation><nlm:affiliation>Department of Chemistry, University of Texas at Dallas, PO Box 830688, Richardson, TX 75083, USA.</nlm:affiliation></affiliation></author><author><name sortKey="Gerard, Robert D" sort="Gerard, Robert D" uniqKey="Gerard R" first="Robert D" last="Gerard">Robert D. Gerard</name></author><author><name sortKey="Zhao, Piyu" sort="Zhao, Piyu" uniqKey="Zhao P" first="Piyu" last="Zhao">Piyu Zhao</name></author><author><name sortKey="Sun, Xiankai" sort="Sun, Xiankai" uniqKey="Sun X" first="Xiankai" last="Sun">Xiankai Sun</name></author><author><name sortKey="Sherry, A Dean" sort="Sherry, A Dean" uniqKey="Sherry A" first="A Dean" last="Sherry">A Dean Sherry</name></author></titleStmt><publicationStmt><idno type="wicri:source">PubMed</idno><date when="2008">2008</date><idno type="doi">10.1021/bc7002605</idno><idno type="RBID">pubmed:18254605</idno><idno type="pmid">18254605</idno><idno type="wicri:Area/PubMed/Corpus">000597</idno><idno type="wicri:explorRef" wicri:stream="PubMed" wicri:step="Corpus" wicri:corpus="PubMed">000597</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en">Labeling of adenovirus particles with PARACEST agents.</title><author><name sortKey="Vasalatiy, Olga" sort="Vasalatiy, Olga" uniqKey="Vasalatiy O" first="Olga" last="Vasalatiy">Olga Vasalatiy</name><affiliation><nlm:affiliation>Department of Chemistry, University of Texas at Dallas, PO Box 830688, Richardson, TX 75083, USA.</nlm:affiliation></affiliation></author><author><name sortKey="Gerard, Robert D" sort="Gerard, Robert D" uniqKey="Gerard R" first="Robert D" last="Gerard">Robert D. Gerard</name></author><author><name sortKey="Zhao, Piyu" sort="Zhao, Piyu" uniqKey="Zhao P" first="Piyu" last="Zhao">Piyu Zhao</name></author><author><name sortKey="Sun, Xiankai" sort="Sun, Xiankai" uniqKey="Sun X" first="Xiankai" last="Sun">Xiankai Sun</name></author><author><name sortKey="Sherry, A Dean" sort="Sherry, A Dean" uniqKey="Sherry A" first="A Dean" last="Sherry">A Dean Sherry</name></author></analytic><series><title level="j">Bioconjugate chemistry</title><idno type="ISSN">1043-1802</idno><imprint><date when="2008" type="published">2008</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Adenoviridae (chemistry)</term><term>Adenoviridae (genetics)</term><term>Adenoviridae Infections (genetics)</term><term>Adenoviridae Infections (virology)</term><term>Cell Line</term><term>Chelating Agents (chemistry)</term><term>Contrast Media (chemistry)</term><term>Crown Compounds (chemistry)</term><term>Humans</term><term>Indicators and Reagents</term><term>Kinetics</term><term>Ligands</term><term>Luciferases (genetics)</term><term>Luminescence</term><term>Lutetium</term><term>Magnetic Resonance Spectroscopy</term><term>Metals (chemistry)</term><term>Radioisotopes</term><term>Spectrometry, Mass, Electrospray Ionization</term><term>Thulium</term></keywords><keywords scheme="MESH" type="chemical" qualifier="chemistry" xml:lang="en"><term>Chelating Agents</term><term>Contrast Media</term><term>Crown Compounds</term><term>Metals</term></keywords><keywords scheme="MESH" qualifier="chemistry" xml:lang="en"><term>Adenoviridae</term></keywords><keywords scheme="MESH" qualifier="genetics" xml:lang="en"><term>Adenoviridae</term><term>Adenoviridae Infections</term><term>Luciferases</term></keywords><keywords scheme="MESH" qualifier="virology" xml:lang="en"><term>Adenoviridae Infections</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Cell Line</term><term>Humans</term><term>Indicators and Reagents</term><term>Kinetics</term><term>Ligands</term><term>Luminescence</term><term>Lutetium</term><term>Magnetic Resonance Spectroscopy</term><term>Radioisotopes</term><term>Spectrometry, Mass, Electrospray Ionization</term><term>Thulium</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Recombinant adenovirus type 5 particles (AdCMVLuc) were labeled with two different bifunctional ligands capable of forming stable complexes with paramagnetic lanthanide ions. The number of covalently attached ligands varied between 630 and 1960 per adenovirus particle depending upon the chemical reactivity of the bifunctional ligand (NHS ester versus isothiocyanide), the amount of excess ligand added, and the reaction time. The bioactivity of each labeled adenovirus derivative, as measured by the ability of the virus to infect cells and express luciferase, was shown to be highly dependent upon the number of covalently attached ligands. This indicates that certain amino groups, likely on the surface of the adenovirus fiber protein where cell binding is known to occur, are critical for viral attachment and infection. Addition of (177)Lu3+ to chemically modified versus control viruses demonstrated a significant amount of nonspecific binding of (177)Lu3+ to the virus particles that could not be sequestered by addition of excess DTPA. Thus, it became necessary to implement a prelabeling strategy for conjugation of preformed lanthanide ligand chelates to adenovirus particles. Using preformed Tm3+- L2, a large number of chelates having chemical exchange saturation transfer (CEST) properties were attached to the surface residues of AdCMVLuc without nonspecific binding of metal ions elsewhere on the virus particle. The potential of such conjugates to act as PARACEST imaging agents was tested using an on-resonance WALTZ sequence for CEST activation. A 12% decrease in bulk water signal intensity was observed relative to controls. This demonstrates that viral particles labeled with PARACEST-type imaging agents can potentially serve as targeted agents for molecular imaging.</div></front></TEI><pubmed><MedlineCitation Owner="NLM" Status="MEDLINE"><PMID Version="1">18254605</PMID><DateCreated><Year>2008</Year><Month>03</Month><Day>20</Day></DateCreated><DateCompleted><Year>2008</Year><Month>06</Month><Day>02</Day></DateCompleted><DateRevised><Year>2015</Year><Month>11</Month><Day>19</Day></DateRevised><Article PubModel="Print-Electronic"><Journal><ISSN IssnType="Print">1043-1802</ISSN><JournalIssue CitedMedium="Print"><Volume>19</Volume><Issue>3</Issue><PubDate><Year>2008</Year><Month>Mar</Month></PubDate></JournalIssue><Title>Bioconjugate chemistry</Title><ISOAbbreviation>Bioconjug. Chem.</ISOAbbreviation></Journal><ArticleTitle>Labeling of adenovirus particles with PARACEST agents.</ArticleTitle><Pagination><MedlinePgn>598-606</MedlinePgn></Pagination><ELocationID EIdType="doi" ValidYN="Y">10.1021/bc7002605</ELocationID><Abstract><AbstractText>Recombinant adenovirus type 5 particles (AdCMVLuc) were labeled with two different bifunctional ligands capable of forming stable complexes with paramagnetic lanthanide ions. The number of covalently attached ligands varied between 630 and 1960 per adenovirus particle depending upon the chemical reactivity of the bifunctional ligand (NHS ester versus isothiocyanide), the amount of excess ligand added, and the reaction time. The bioactivity of each labeled adenovirus derivative, as measured by the ability of the virus to infect cells and express luciferase, was shown to be highly dependent upon the number of covalently attached ligands. This indicates that certain amino groups, likely on the surface of the adenovirus fiber protein where cell binding is known to occur, are critical for viral attachment and infection. Addition of (177)Lu3+ to chemically modified versus control viruses demonstrated a significant amount of nonspecific binding of (177)Lu3+ to the virus particles that could not be sequestered by addition of excess DTPA. Thus, it became necessary to implement a prelabeling strategy for conjugation of preformed lanthanide ligand chelates to adenovirus particles. Using preformed Tm3+- L2, a large number of chelates having chemical exchange saturation transfer (CEST) properties were attached to the surface residues of AdCMVLuc without nonspecific binding of metal ions elsewhere on the virus particle. The potential of such conjugates to act as PARACEST imaging agents was tested using an on-resonance WALTZ sequence for CEST activation. A 12% decrease in bulk water signal intensity was observed relative to controls. This demonstrates that viral particles labeled with PARACEST-type imaging agents can potentially serve as targeted agents for molecular imaging.</AbstractText></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Vasalatiy</LastName><ForeName>Olga</ForeName><Initials>O</Initials><AffiliationInfo><Affiliation>Department of Chemistry, University of Texas at Dallas, PO Box 830688, Richardson, TX 75083, USA.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Gerard</LastName><ForeName>Robert D</ForeName><Initials>RD</Initials></Author><Author ValidYN="Y"><LastName>Zhao</LastName><ForeName>Piyu</ForeName><Initials>P</Initials></Author><Author ValidYN="Y"><LastName>Sun</LastName><ForeName>Xiankai</ForeName><Initials>X</Initials></Author><Author ValidYN="Y"><LastName>Sherry</LastName><ForeName>A Dean</ForeName><Initials>AD</Initials></Author></AuthorList><Language>eng</Language><GrantList CompleteYN="Y"><Grant><GrantID>CA-115531</GrantID><Acronym>CA</Acronym><Agency>NCI NIH HHS</Agency><Country>United States</Country></Grant><Grant><GrantID>P41 RR002584</GrantID><Acronym>RR</Acronym><Agency>NCRR NIH HHS</Agency><Country>United States</Country></Grant><Grant><GrantID>P41 RR002584-216319</GrantID><Acronym>RR</Acronym><Agency>NCRR NIH HHS</Agency><Country>United States</Country></Grant><Grant><GrantID>R01 CA115531</GrantID><Acronym>CA</Acronym><Agency>NCI NIH HHS</Agency><Country>United States</Country></Grant><Grant><GrantID>R01 CA115531-02</GrantID><Acronym>CA</Acronym><Agency>NCI NIH HHS</Agency><Country>United States</Country></Grant><Grant><GrantID>RR-02584</GrantID><Acronym>RR</Acronym><Agency>NCRR NIH HHS</Agency><Country>United States</Country></Grant></GrantList><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType><PublicationType UI="D052061">Research Support, N.I.H., Extramural</PublicationType><PublicationType UI="D013485">Research Support, Non-U.S. Gov't</PublicationType></PublicationTypeList><ArticleDate DateType="Electronic"><Year>2008</Year><Month>02</Month><Day>07</Day></ArticleDate></Article><MedlineJournalInfo><Country>United States</Country><MedlineTA>Bioconjug Chem</MedlineTA><NlmUniqueID>9010319</NlmUniqueID><ISSNLinking>1043-1802</ISSNLinking></MedlineJournalInfo><ChemicalList><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D002614">Chelating Agents</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D003287">Contrast Media</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D047248">Crown Compounds</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D007202">Indicators and Reagents</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance 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RefType="Cites"><RefSource>Bioconjug Chem. 2001 Mar-Apr;12(2):320-4</RefSource><PMID Version="1">11312695</PMID></CommentsCorrections></CommentsCorrectionsList><MeshHeadingList><MeshHeading><DescriptorName MajorTopicYN="N" UI="D000256">Adenoviridae</DescriptorName><QualifierName MajorTopicYN="Y" UI="Q000737">chemistry</QualifierName><QualifierName MajorTopicYN="N" UI="Q000235">genetics</QualifierName></MeshHeading><MeshHeading><DescriptorName MajorTopicYN="N" UI="D000257">Adenoviridae Infections</DescriptorName><QualifierName MajorTopicYN="N" UI="Q000235">genetics</QualifierName><QualifierName MajorTopicYN="N" UI="Q000821">virology</QualifierName></MeshHeading><MeshHeading><DescriptorName MajorTopicYN="N" UI="D002460">Cell Line</DescriptorName></MeshHeading><MeshHeading><DescriptorName MajorTopicYN="N" UI="D002614">Chelating Agents</DescriptorName><QualifierName MajorTopicYN="N" UI="Q000737">chemistry</QualifierName></MeshHeading><MeshHeading><DescriptorName MajorTopicYN="N" UI="D003287">Contrast Media</DescriptorName><QualifierName MajorTopicYN="Y" UI="Q000737">chemistry</QualifierName></MeshHeading><MeshHeading><DescriptorName MajorTopicYN="N" UI="D047248">Crown Compounds</DescriptorName><QualifierName MajorTopicYN="Y" UI="Q000737">chemistry</QualifierName></MeshHeading><MeshHeading><DescriptorName MajorTopicYN="N" UI="D006801">Humans</DescriptorName></MeshHeading><MeshHeading><DescriptorName MajorTopicYN="N" UI="D007202">Indicators and Reagents</DescriptorName></MeshHeading><MeshHeading><DescriptorName MajorTopicYN="N" UI="D007700">Kinetics</DescriptorName></MeshHeading><MeshHeading><DescriptorName MajorTopicYN="N" UI="D008024">Ligands</DescriptorName></MeshHeading><MeshHeading><DescriptorName MajorTopicYN="N" UI="D008156">Luciferases</DescriptorName><QualifierName MajorTopicYN="N" UI="Q000235">genetics</QualifierName></MeshHeading><MeshHeading><DescriptorName MajorTopicYN="N" 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