High contrast in vitro and in vivo photoluminescence bioimaging using near infrared to near infrared up-conversion in Tm3+ and Yb3+ doped fluoride nanophosphors.
Identifieur interne : 000564 ( PubMed/Corpus ); précédent : 000563; suivant : 000565High contrast in vitro and in vivo photoluminescence bioimaging using near infrared to near infrared up-conversion in Tm3+ and Yb3+ doped fluoride nanophosphors.
Auteurs : Marcin Nyk ; Rajiv Kumar ; Tymish Y. Ohulchanskyy ; Earl J. Bergey ; Paras N. PrasadSource :
- Nano letters [ 1530-6984 ] ; 2008.
English descriptors
- KwdEn :
- Animals, Biosensing Techniques (methods), Cations (chemistry), Cell Line, Cell Survival, Fluorides (chemistry), Luminescent Measurements, Mice, Mice, Inbred BALB C, Microscopy, Electron, Transmission, Nanoparticles (chemistry), Nanoparticles (ultrastructure), Photochemistry, Thulium (chemistry), X-Ray Diffraction, Ytterbium (chemistry).
- MESH :
- chemical , chemistry : Cations, Fluorides, Thulium, Ytterbium.
- chemistry : Nanoparticles.
- methods : Biosensing Techniques.
- ultrastructure : Nanoparticles.
- Animals, Cell Line, Cell Survival, Luminescent Measurements, Mice, Mice, Inbred BALB C, Microscopy, Electron, Transmission, Photochemistry, X-Ray Diffraction.
Abstract
A new approach for photoluminescence imaging in vitro and in vivo has been shown utilizing near infrared to near infrared (NIR-to-NIR) up-conversion in nanophosphors. This NIR-to-NIR up-conversion process provides deeper light penetration into biological specimen and results in high contrast optical imaging due to absence of an autofluorescence background and decreased light scattering. Aqueous dispersible fluoride (NaYF4) nanocrystals (20-30 nm size) co-doped with the rare earth ions, Tm(3+) and Yb(3+), were synthesized and characterized by TEM, XRD, and photoluminescence (PL) spectroscopy. In vitro cellular uptake was shown by the PL microscopy visualizing the characteristic emission of Tm(3+) at approximately 800 nm excited with 975 nm. No apparent cytotoxicity was observed. Subsequent animal imaging studies were performed using Balb-c mice injected intravenously with up-converting nanophosphors, demonstrating the high contrast PL imaging in vivo.
DOI: 10.1021/nl802223f
PubMed: 18928324
Links to Exploration step
pubmed:18928324Le document en format XML
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Ohulchanskyy</name></author><author><name sortKey="Bergey, Earl J" sort="Bergey, Earl J" uniqKey="Bergey E" first="Earl J" last="Bergey">Earl J. Bergey</name></author><author><name sortKey="Prasad, Paras N" sort="Prasad, Paras N" uniqKey="Prasad P" first="Paras N" last="Prasad">Paras N. Prasad</name></author></titleStmt><publicationStmt><idno type="wicri:source">PubMed</idno><date when="2008">2008</date><idno type="doi">10.1021/nl802223f</idno><idno type="RBID">pubmed:18928324</idno><idno type="pmid">18928324</idno><idno type="wicri:Area/PubMed/Corpus">000564</idno><idno type="wicri:explorRef" wicri:stream="PubMed" wicri:step="Corpus" wicri:corpus="PubMed">000564</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en">High contrast in vitro and in vivo photoluminescence bioimaging using near infrared to near infrared up-conversion in Tm3+ and Yb3+ doped fluoride nanophosphors.</title><author><name sortKey="Nyk, Marcin" sort="Nyk, Marcin" uniqKey="Nyk M" first="Marcin" last="Nyk">Marcin Nyk</name><affiliation><nlm:affiliation>Institute for Lasers, Photonics and Biophotonics, Department of Chemistry, University at Buffalo, State University of New York, Buffalo, New York 14260-3000, USA.</nlm:affiliation></affiliation></author><author><name sortKey="Kumar, Rajiv" sort="Kumar, Rajiv" uniqKey="Kumar R" first="Rajiv" last="Kumar">Rajiv Kumar</name></author><author><name sortKey="Ohulchanskyy, Tymish Y" sort="Ohulchanskyy, Tymish Y" uniqKey="Ohulchanskyy T" first="Tymish Y" last="Ohulchanskyy">Tymish Y. Ohulchanskyy</name></author><author><name sortKey="Bergey, Earl J" sort="Bergey, Earl J" uniqKey="Bergey E" first="Earl J" last="Bergey">Earl J. Bergey</name></author><author><name sortKey="Prasad, Paras N" sort="Prasad, Paras N" uniqKey="Prasad P" first="Paras N" last="Prasad">Paras N. Prasad</name></author></analytic><series><title level="j">Nano letters</title><idno type="ISSN">1530-6984</idno><imprint><date when="2008" type="published">2008</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Animals</term><term>Biosensing Techniques (methods)</term><term>Cations (chemistry)</term><term>Cell Line</term><term>Cell Survival</term><term>Fluorides (chemistry)</term><term>Luminescent Measurements</term><term>Mice</term><term>Mice, Inbred BALB C</term><term>Microscopy, Electron, Transmission</term><term>Nanoparticles (chemistry)</term><term>Nanoparticles (ultrastructure)</term><term>Photochemistry</term><term>Thulium (chemistry)</term><term>X-Ray Diffraction</term><term>Ytterbium (chemistry)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="chemistry" xml:lang="en"><term>Cations</term><term>Fluorides</term><term>Thulium</term><term>Ytterbium</term></keywords><keywords scheme="MESH" qualifier="chemistry" xml:lang="en"><term>Nanoparticles</term></keywords><keywords scheme="MESH" qualifier="methods" xml:lang="en"><term>Biosensing Techniques</term></keywords><keywords scheme="MESH" qualifier="ultrastructure" xml:lang="en"><term>Nanoparticles</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Animals</term><term>Cell Line</term><term>Cell Survival</term><term>Luminescent Measurements</term><term>Mice</term><term>Mice, Inbred BALB C</term><term>Microscopy, Electron, Transmission</term><term>Photochemistry</term><term>X-Ray Diffraction</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">A new approach for photoluminescence imaging in vitro and in vivo has been shown utilizing near infrared to near infrared (NIR-to-NIR) up-conversion in nanophosphors. This NIR-to-NIR up-conversion process provides deeper light penetration into biological specimen and results in high contrast optical imaging due to absence of an autofluorescence background and decreased light scattering. Aqueous dispersible fluoride (NaYF4) nanocrystals (20-30 nm size) co-doped with the rare earth ions, Tm(3+) and Yb(3+), were synthesized and characterized by TEM, XRD, and photoluminescence (PL) spectroscopy. In vitro cellular uptake was shown by the PL microscopy visualizing the characteristic emission of Tm(3+) at approximately 800 nm excited with 975 nm. No apparent cytotoxicity was observed. Subsequent animal imaging studies were performed using Balb-c mice injected intravenously with up-converting nanophosphors, demonstrating the high contrast PL imaging in vivo.</div></front></TEI><pubmed><MedlineCitation Owner="NLM" Status="MEDLINE"><PMID Version="1">18928324</PMID><DateCreated><Year>2008</Year><Month>11</Month><Day>13</Day></DateCreated><DateCompleted><Year>2008</Year><Month>12</Month><Day>24</Day></DateCompleted><DateRevised><Year>2014</Year><Month>09</Month><Day>03</Day></DateRevised><Article PubModel="Print-Electronic"><Journal><ISSN IssnType="Print">1530-6984</ISSN><JournalIssue CitedMedium="Print"><Volume>8</Volume><Issue>11</Issue><PubDate><Year>2008</Year><Month>Nov</Month></PubDate></JournalIssue><Title>Nano letters</Title><ISOAbbreviation>Nano Lett.</ISOAbbreviation></Journal><ArticleTitle>High contrast in vitro and in vivo photoluminescence bioimaging using near infrared to near infrared up-conversion in Tm3+ and Yb3+ doped fluoride nanophosphors.</ArticleTitle><Pagination><MedlinePgn>3834-8</MedlinePgn></Pagination><ELocationID EIdType="doi" ValidYN="Y">10.1021/nl802223f</ELocationID><Abstract><AbstractText>A new approach for photoluminescence imaging in vitro and in vivo has been shown utilizing near infrared to near infrared (NIR-to-NIR) up-conversion in nanophosphors. This NIR-to-NIR up-conversion process provides deeper light penetration into biological specimen and results in high contrast optical imaging due to absence of an autofluorescence background and decreased light scattering. Aqueous dispersible fluoride (NaYF4) nanocrystals (20-30 nm size) co-doped with the rare earth ions, Tm(3+) and Yb(3+), were synthesized and characterized by TEM, XRD, and photoluminescence (PL) spectroscopy. In vitro cellular uptake was shown by the PL microscopy visualizing the characteristic emission of Tm(3+) at approximately 800 nm excited with 975 nm. No apparent cytotoxicity was observed. Subsequent animal imaging studies were performed using Balb-c mice injected intravenously with up-converting nanophosphors, demonstrating the high contrast PL imaging in vivo.</AbstractText></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Nyk</LastName><ForeName>Marcin</ForeName><Initials>M</Initials><AffiliationInfo><Affiliation>Institute for Lasers, Photonics and Biophotonics, Department of Chemistry, University at Buffalo, State University of New York, Buffalo, New York 14260-3000, USA.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Kumar</LastName><ForeName>Rajiv</ForeName><Initials>R</Initials></Author><Author ValidYN="Y"><LastName>Ohulchanskyy</LastName><ForeName>Tymish Y</ForeName><Initials>TY</Initials></Author><Author ValidYN="Y"><LastName>Bergey</LastName><ForeName>Earl J</ForeName><Initials>EJ</Initials></Author><Author ValidYN="Y"><LastName>Prasad</LastName><ForeName>Paras 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24;125(38):11474-5</RefSource><PMID Version="1">13129331</PMID></CommentsCorrections><CommentsCorrections RefType="Cites"><RefSource>Nat Biotechnol. 2004 Jan;22(1):47-52</RefSource><PMID Version="1">14704706</PMID></CommentsCorrections><CommentsCorrections RefType="Cites"><RefSource>Nano Lett. 2005 Jan;5(1):113-7</RefSource><PMID Version="1">15792423</PMID></CommentsCorrections><CommentsCorrections RefType="Cites"><RefSource>Environ Health Perspect. 2006 Feb;114(2):165-72</RefSource><PMID Version="1">16451849</PMID></CommentsCorrections><CommentsCorrections RefType="Cites"><RefSource>Nano Lett. 2006 Feb;6(2):169-74</RefSource><PMID Version="1">16464029</PMID></CommentsCorrections><CommentsCorrections RefType="Cites"><RefSource>Annu Rev Biomed Eng. 2000;2:399-429</RefSource><PMID Version="1">11701518</PMID></CommentsCorrections></CommentsCorrectionsList><MeshHeadingList><MeshHeading><DescriptorName MajorTopicYN="N" 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