A dual enzyme functionalized nanostructured thulium oxide based interface for biomedical application.
Identifieur interne : 000221 ( PubMed/Corpus ); précédent : 000220; suivant : 000222A dual enzyme functionalized nanostructured thulium oxide based interface for biomedical application.
Auteurs : Jay Singh ; Appan Roychoudhury ; Manish Srivastava ; Pratima R. Solanki ; Dong Won Lee ; Seung Hee Lee ; B D MalhotraSource :
- Nanoscale [ 2040-3372 ] ; 2014.
English descriptors
- KwdEn :
- Biosensing Techniques, Cholesterol (blood), Cholesterol Oxidase (chemistry), Cholesterol Oxidase (metabolism), Electrochemical Techniques, Electrodes, Enzymes, Immobilized (chemistry), Enzymes, Immobilized (metabolism), Glass (chemistry), Humans, Kinetics, Nanostructures (chemistry), Nanotubes (chemistry), Sterol Esterase (chemistry), Sterol Esterase (metabolism), Thulium (chemistry), Tin Compounds (chemistry).
- MESH :
- chemical , blood : Cholesterol.
- chemical , chemistry : Cholesterol Oxidase, Enzymes, Immobilized, Sterol Esterase, Thulium, Tin Compounds.
- chemical , metabolism : Cholesterol Oxidase, Enzymes, Immobilized, Sterol Esterase.
- chemistry : Glass, Nanostructures, Nanotubes.
- Biosensing Techniques, Electrochemical Techniques, Electrodes, Humans, Kinetics.
Abstract
In this paper, we present results of the studies related to fabrication of a rare earth metal oxide based efficient biosensor using an interface based on hydrothermally prepared nanostructured thulium oxide (n-Tm2O3). A colloidal solution of prepared nanorods has been electrophoretically deposited (EPD) onto an indium-tin-oxide (ITO) glass substrate. The n-Tm2O3 nanorods are found to provide improved sensing characteristics to the electrode interface in terms of electroactive surface area, diffusion coefficient, charge transfer rate constant and electron transfer kinetics. The structural and morphological studies of n-Tm2O3 nanorods have been carried out by X-ray diffraction (XRD), scanning electron microscopy (SEM), transmission electron microscopy (TEM), X-ray photoelectron spectroscopy (XPS) and Fourier transform infrared (FTIR) spectroscopic techniques. This interfacial platform has been used for fabrication of a total cholesterol biosensor by immobilizing cholesterol esterase (ChEt) and cholesterol oxidase (ChOx) onto a Tm2O3 nanostructured surface. The results of response studies of the fabricated ChEt-ChOx/n-Tm2O3/ITO bioelectrode show a broad linear range of 8-400 mg dL(-1), detection limit of 19.78 mg (dL cm(-2))(-1), and high sensitivity of 0.9245 μA (mg per dL cm(-2))(-1) with a response time of 40 s. Further, this bioelectrode has been utilized for estimation of total cholesterol with negligible interference (3%) from analytes present in human serum samples. The utilization of this n-Tm2O3 modified electrode for enzyme-based biosensor analysis offers an efficient strategy and a novel interface for application of the rare earth metal oxide materials in the field of electrochemical sensors and bioelectronic devices.
DOI: 10.1039/c3nr05043b
PubMed: 24301799
Links to Exploration step
pubmed:24301799Le document en format XML
<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">A dual enzyme functionalized nanostructured thulium oxide based interface for biomedical application.</title><author><name sortKey="Singh, Jay" sort="Singh, Jay" uniqKey="Singh J" first="Jay" last="Singh">Jay Singh</name><affiliation><nlm:affiliation>Department of BIN Fusion Technology, Department of Polymer-Nano Science and Technology, Chonbuk National University, Jeonju, Jeonbuk 561-756, Korea. lsh1@jbnu.ac.kr.</nlm:affiliation></affiliation></author><author><name sortKey="Roychoudhury, Appan" sort="Roychoudhury, Appan" uniqKey="Roychoudhury A" first="Appan" last="Roychoudhury">Appan Roychoudhury</name></author><author><name sortKey="Srivastava, Manish" sort="Srivastava, Manish" uniqKey="Srivastava M" first="Manish" last="Srivastava">Manish Srivastava</name></author><author><name sortKey="Solanki, Pratima R" sort="Solanki, Pratima R" uniqKey="Solanki P" first="Pratima R" last="Solanki">Pratima R. Solanki</name></author><author><name sortKey="Lee, Dong Won" sort="Lee, Dong Won" uniqKey="Lee D" first="Dong Won" last="Lee">Dong Won Lee</name></author><author><name sortKey="Lee, Seung Hee" sort="Lee, Seung Hee" uniqKey="Lee S" first="Seung Hee" last="Lee">Seung Hee Lee</name></author><author><name sortKey="Malhotra, B D" sort="Malhotra, B D" uniqKey="Malhotra B" first="B D" last="Malhotra">B D Malhotra</name></author></titleStmt><publicationStmt><idno type="wicri:source">PubMed</idno><date when="2014">2014</date><idno type="doi">10.1039/c3nr05043b</idno><idno type="RBID">pubmed:24301799</idno><idno type="pmid">24301799</idno><idno type="wicri:Area/PubMed/Corpus">000221</idno><idno type="wicri:explorRef" wicri:stream="PubMed" wicri:step="Corpus" wicri:corpus="PubMed">000221</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en">A dual enzyme functionalized nanostructured thulium oxide based interface for biomedical application.</title><author><name sortKey="Singh, Jay" sort="Singh, Jay" uniqKey="Singh J" first="Jay" last="Singh">Jay Singh</name><affiliation><nlm:affiliation>Department of BIN Fusion Technology, Department of Polymer-Nano Science and Technology, Chonbuk National University, Jeonju, Jeonbuk 561-756, Korea. lsh1@jbnu.ac.kr.</nlm:affiliation></affiliation></author><author><name sortKey="Roychoudhury, Appan" sort="Roychoudhury, Appan" uniqKey="Roychoudhury A" first="Appan" last="Roychoudhury">Appan Roychoudhury</name></author><author><name sortKey="Srivastava, Manish" sort="Srivastava, Manish" uniqKey="Srivastava M" first="Manish" last="Srivastava">Manish Srivastava</name></author><author><name sortKey="Solanki, Pratima R" sort="Solanki, Pratima R" uniqKey="Solanki P" first="Pratima R" last="Solanki">Pratima R. Solanki</name></author><author><name sortKey="Lee, Dong Won" sort="Lee, Dong Won" uniqKey="Lee D" first="Dong Won" last="Lee">Dong Won Lee</name></author><author><name sortKey="Lee, Seung Hee" sort="Lee, Seung Hee" uniqKey="Lee S" first="Seung Hee" last="Lee">Seung Hee Lee</name></author><author><name sortKey="Malhotra, B D" sort="Malhotra, B D" uniqKey="Malhotra B" first="B D" last="Malhotra">B D Malhotra</name></author></analytic><series><title level="j">Nanoscale</title><idno type="eISSN">2040-3372</idno><imprint><date when="2014" type="published">2014</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Biosensing Techniques</term><term>Cholesterol (blood)</term><term>Cholesterol Oxidase (chemistry)</term><term>Cholesterol Oxidase (metabolism)</term><term>Electrochemical Techniques</term><term>Electrodes</term><term>Enzymes, Immobilized (chemistry)</term><term>Enzymes, Immobilized (metabolism)</term><term>Glass (chemistry)</term><term>Humans</term><term>Kinetics</term><term>Nanostructures (chemistry)</term><term>Nanotubes (chemistry)</term><term>Sterol Esterase (chemistry)</term><term>Sterol Esterase (metabolism)</term><term>Thulium (chemistry)</term><term>Tin Compounds (chemistry)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="blood" xml:lang="en"><term>Cholesterol</term></keywords><keywords scheme="MESH" type="chemical" qualifier="chemistry" xml:lang="en"><term>Cholesterol Oxidase</term><term>Enzymes, Immobilized</term><term>Sterol Esterase</term><term>Thulium</term><term>Tin Compounds</term></keywords><keywords scheme="MESH" type="chemical" qualifier="metabolism" xml:lang="en"><term>Cholesterol Oxidase</term><term>Enzymes, Immobilized</term><term>Sterol Esterase</term></keywords><keywords scheme="MESH" qualifier="chemistry" xml:lang="en"><term>Glass</term><term>Nanostructures</term><term>Nanotubes</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Biosensing Techniques</term><term>Electrochemical Techniques</term><term>Electrodes</term><term>Humans</term><term>Kinetics</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">In this paper, we present results of the studies related to fabrication of a rare earth metal oxide based efficient biosensor using an interface based on hydrothermally prepared nanostructured thulium oxide (n-Tm2O3). A colloidal solution of prepared nanorods has been electrophoretically deposited (EPD) onto an indium-tin-oxide (ITO) glass substrate. The n-Tm2O3 nanorods are found to provide improved sensing characteristics to the electrode interface in terms of electroactive surface area, diffusion coefficient, charge transfer rate constant and electron transfer kinetics. The structural and morphological studies of n-Tm2O3 nanorods have been carried out by X-ray diffraction (XRD), scanning electron microscopy (SEM), transmission electron microscopy (TEM), X-ray photoelectron spectroscopy (XPS) and Fourier transform infrared (FTIR) spectroscopic techniques. This interfacial platform has been used for fabrication of a total cholesterol biosensor by immobilizing cholesterol esterase (ChEt) and cholesterol oxidase (ChOx) onto a Tm2O3 nanostructured surface. The results of response studies of the fabricated ChEt-ChOx/n-Tm2O3/ITO bioelectrode show a broad linear range of 8-400 mg dL(-1), detection limit of 19.78 mg (dL cm(-2))(-1), and high sensitivity of 0.9245 μA (mg per dL cm(-2))(-1) with a response time of 40 s. Further, this bioelectrode has been utilized for estimation of total cholesterol with negligible interference (3%) from analytes present in human serum samples. The utilization of this n-Tm2O3 modified electrode for enzyme-based biosensor analysis offers an efficient strategy and a novel interface for application of the rare earth metal oxide materials in the field of electrochemical sensors and bioelectronic devices.</div></front></TEI><pubmed><MedlineCitation Owner="NLM" Status="MEDLINE"><PMID Version="1">24301799</PMID><DateCreated><Year>2013</Year><Month>12</Month><Day>20</Day></DateCreated><DateCompleted><Year>2014</Year><Month>08</Month><Day>12</Day></DateCompleted><Article PubModel="Print"><Journal><ISSN IssnType="Electronic">2040-3372</ISSN><JournalIssue CitedMedium="Internet"><Volume>6</Volume><Issue>2</Issue><PubDate><Year>2014</Year><Month>Jan</Month><Day>21</Day></PubDate></JournalIssue><Title>Nanoscale</Title><ISOAbbreviation>Nanoscale</ISOAbbreviation></Journal><ArticleTitle>A dual enzyme functionalized nanostructured thulium oxide based interface for biomedical application.</ArticleTitle><Pagination><MedlinePgn>1195-208</MedlinePgn></Pagination><ELocationID EIdType="doi" ValidYN="Y">10.1039/c3nr05043b</ELocationID><Abstract><AbstractText>In this paper, we present results of the studies related to fabrication of a rare earth metal oxide based efficient biosensor using an interface based on hydrothermally prepared nanostructured thulium oxide (n-Tm2O3). A colloidal solution of prepared nanorods has been electrophoretically deposited (EPD) onto an indium-tin-oxide (ITO) glass substrate. The n-Tm2O3 nanorods are found to provide improved sensing characteristics to the electrode interface in terms of electroactive surface area, diffusion coefficient, charge transfer rate constant and electron transfer kinetics. The structural and morphological studies of n-Tm2O3 nanorods have been carried out by X-ray diffraction (XRD), scanning electron microscopy (SEM), transmission electron microscopy (TEM), X-ray photoelectron spectroscopy (XPS) and Fourier transform infrared (FTIR) spectroscopic techniques. This interfacial platform has been used for fabrication of a total cholesterol biosensor by immobilizing cholesterol esterase (ChEt) and cholesterol oxidase (ChOx) onto a Tm2O3 nanostructured surface. The results of response studies of the fabricated ChEt-ChOx/n-Tm2O3/ITO bioelectrode show a broad linear range of 8-400 mg dL(-1), detection limit of 19.78 mg (dL cm(-2))(-1), and high sensitivity of 0.9245 μA (mg per dL cm(-2))(-1) with a response time of 40 s. Further, this bioelectrode has been utilized for estimation of total cholesterol with negligible interference (3%) from analytes present in human serum samples. The utilization of this n-Tm2O3 modified electrode for enzyme-based biosensor analysis offers an efficient strategy and a novel interface for application of the rare earth metal oxide materials in the field of electrochemical sensors and bioelectronic devices.</AbstractText></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Singh</LastName><ForeName>Jay</ForeName><Initials>J</Initials><AffiliationInfo><Affiliation>Department of BIN Fusion Technology, Department of Polymer-Nano Science and Technology, Chonbuk National University, Jeonju, Jeonbuk 561-756, Korea. lsh1@jbnu.ac.kr.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Roychoudhury</LastName><ForeName>Appan</ForeName><Initials>A</Initials></Author><Author ValidYN="Y"><LastName>Srivastava</LastName><ForeName>Manish</ForeName><Initials>M</Initials></Author><Author ValidYN="Y"><LastName>Solanki</LastName><ForeName>Pratima R</ForeName><Initials>PR</Initials></Author><Author ValidYN="Y"><LastName>Lee</LastName><ForeName>Dong Won</ForeName><Initials>DW</Initials></Author><Author ValidYN="Y"><LastName>Lee</LastName><ForeName>Seung Hee</ForeName><Initials>SH</Initials></Author><Author ValidYN="Y"><LastName>Malhotra</LastName><ForeName>B D</ForeName><Initials>BD</Initials></Author></AuthorList><Language>eng</Language><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType><PublicationType UI="D013485">Research Support, Non-U.S. Gov't</PublicationType></PublicationTypeList></Article><MedlineJournalInfo><Country>England</Country><MedlineTA>Nanoscale</MedlineTA><NlmUniqueID>101525249</NlmUniqueID><ISSNLinking>2040-3364</ISSNLinking></MedlineJournalInfo><ChemicalList><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D004800">Enzymes, Immobilized</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D017971">Tin Compounds</NameOfSubstance></Chemical><Chemical><RegistryNumber>12036-44-1</RegistryNumber><NameOfSubstance UI="C033160">thulium oxide</NameOfSubstance></Chemical><Chemical><RegistryNumber>71243-84-0</RegistryNumber><NameOfSubstance UI="C109984">indium tin oxide</NameOfSubstance></Chemical><Chemical><RegistryNumber>8RKC5ATI4P</RegistryNumber><NameOfSubstance UI="D013932">Thulium</NameOfSubstance></Chemical><Chemical><RegistryNumber>97C5T2UQ7J</RegistryNumber><NameOfSubstance UI="D002784">Cholesterol</NameOfSubstance></Chemical><Chemical><RegistryNumber>EC 1.1.3.6</RegistryNumber><NameOfSubstance UI="D002789">Cholesterol Oxidase</NameOfSubstance></Chemical><Chemical><RegistryNumber>EC 3.1.1.13</RegistryNumber><NameOfSubstance UI="D002787">Sterol Esterase</NameOfSubstance></Chemical></ChemicalList><CitationSubset>IM</CitationSubset><MeshHeadingList><MeshHeading><DescriptorName MajorTopicYN="N" UI="D015374">Biosensing Techniques</DescriptorName></MeshHeading><MeshHeading><DescriptorName MajorTopicYN="N" UI="D002784">Cholesterol</DescriptorName><QualifierName MajorTopicYN="N" UI="Q000097">blood</QualifierName></MeshHeading><MeshHeading><DescriptorName MajorTopicYN="N" UI="D002789">Cholesterol Oxidase</DescriptorName><QualifierName MajorTopicYN="N" UI="Q000737">chemistry</QualifierName><QualifierName MajorTopicYN="Y" UI="Q000378">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName MajorTopicYN="N" UI="D055664">Electrochemical Techniques</DescriptorName></MeshHeading><MeshHeading><DescriptorName MajorTopicYN="N" UI="D004566">Electrodes</DescriptorName></MeshHeading><MeshHeading><DescriptorName MajorTopicYN="N" UI="D004800">Enzymes, Immobilized</DescriptorName><QualifierName MajorTopicYN="N" UI="Q000737">chemistry</QualifierName><QualifierName MajorTopicYN="N" UI="Q000378">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName MajorTopicYN="N" UI="D005898">Glass</DescriptorName><QualifierName MajorTopicYN="N" UI="Q000737">chemistry</QualifierName></MeshHeading><MeshHeading><DescriptorName MajorTopicYN="N" UI="D006801">Humans</DescriptorName></MeshHeading><MeshHeading><DescriptorName MajorTopicYN="N" UI="D007700">Kinetics</DescriptorName></MeshHeading><MeshHeading><DescriptorName MajorTopicYN="N" UI="D049329">Nanostructures</DescriptorName><QualifierName MajorTopicYN="Y" UI="Q000737">chemistry</QualifierName></MeshHeading><MeshHeading><DescriptorName MajorTopicYN="N" UI="D043942">Nanotubes</DescriptorName><QualifierName MajorTopicYN="N" UI="Q000737">chemistry</QualifierName></MeshHeading><MeshHeading><DescriptorName MajorTopicYN="N" UI="D002787">Sterol Esterase</DescriptorName><QualifierName MajorTopicYN="N" UI="Q000737">chemistry</QualifierName><QualifierName MajorTopicYN="Y" UI="Q000378">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName MajorTopicYN="N" UI="D013932">Thulium</DescriptorName><QualifierName MajorTopicYN="Y" UI="Q000737">chemistry</QualifierName></MeshHeading><MeshHeading><DescriptorName MajorTopicYN="N" UI="D017971">Tin Compounds</DescriptorName><QualifierName MajorTopicYN="N" UI="Q000737">chemistry</QualifierName></MeshHeading></MeshHeadingList></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="entrez"><Year>2013</Year><Month>12</Month><Day>5</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="pubmed"><Year>2013</Year><Month>12</Month><Day>5</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="medline"><Year>2014</Year><Month>8</Month><Day>13</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate></History><PublicationStatus>ppublish</PublicationStatus><ArticleIdList><ArticleId IdType="doi">10.1039/c3nr05043b</ArticleId><ArticleId IdType="pubmed">24301799</ArticleId></ArticleIdList></PubmedData></pubmed></record>Pour manipuler ce document sous Unix (Dilib)
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