Mesoporous silica-coated upconversion nanocrystals for near infrared light-triggered control of gene expression in zebrafish.
Identifieur interne : 000085 ( PubMed/Corpus ); précédent : 000084; suivant : 000086Mesoporous silica-coated upconversion nanocrystals for near infrared light-triggered control of gene expression in zebrafish.
Auteurs : Muthu Kumara Gnanasammandhan Jayakumar ; Akshaya Bansal ; Bing Nan Li ; Yong ZhangSource :
- Nanomedicine (London, England) [ 1748-6963 ] ; 2015.
English descriptors
- KwdEn :
- Animals, DNA (administration & dosage), DNA (genetics), Gene Knockdown Techniques (methods), Gene Transfer Techniques, Infrared Rays, Morpholinos (administration & dosage), Morpholinos (genetics), Nanoparticles (chemistry), Nanoparticles (ultrastructure), Nanotechnology, Photosensitizing Agents (chemistry), Plasmids (administration & dosage), Plasmids (genetics), Silicon Dioxide (chemistry), Thulium (chemistry), Ultraviolet Rays, Ytterbium (chemistry), Yttrium (chemistry), Zebrafish (embryology), Zebrafish (genetics).
- MESH :
- chemical , administration & dosage : DNA, Morpholinos.
- chemical , chemistry : Photosensitizing Agents, Silicon Dioxide, Thulium, Ytterbium, Yttrium.
- chemical , genetics : DNA, Morpholinos.
- administration & dosage : Plasmids.
- chemistry : Nanoparticles.
- embryology : Zebrafish.
- genetics : Plasmids, Zebrafish.
- methods : Gene Knockdown Techniques.
- ultrastructure : Nanoparticles.
- Animals, Gene Transfer Techniques, Infrared Rays, Nanotechnology, Ultraviolet Rays.
Abstract
To develop a platform technology for photoactivation of gene expression in deep tissues.
DOI: 10.2217/nnm.14.198
PubMed: 25929564
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<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">Mesoporous silica-coated upconversion nanocrystals for near infrared light-triggered control of gene expression in zebrafish.</title><author><name sortKey="Jayakumar, Muthu Kumara Gnanasammandhan" sort="Jayakumar, Muthu Kumara Gnanasammandhan" uniqKey="Jayakumar M" first="Muthu Kumara Gnanasammandhan" last="Jayakumar">Muthu Kumara Gnanasammandhan Jayakumar</name><affiliation><nlm:affiliation>Department of Biomedical Engineering, Faculty of Engineering, Block EA #03-12, National University of Singapore, 9 Engineering Drive 1, 117576, Singapore.</nlm:affiliation></affiliation></author><author><name sortKey="Bansal, Akshaya" sort="Bansal, Akshaya" uniqKey="Bansal A" first="Akshaya" last="Bansal">Akshaya Bansal</name></author><author><name sortKey="Li, Bing Nan" sort="Li, Bing Nan" uniqKey="Li B" first="Bing Nan" last="Li">Bing Nan Li</name></author><author><name sortKey="Zhang, Yong" sort="Zhang, Yong" uniqKey="Zhang Y" first="Yong" last="Zhang">Yong Zhang</name></author></titleStmt><publicationStmt><idno type="wicri:source">PubMed</idno><date when="2015">2015</date><idno type="doi">10.2217/nnm.14.198</idno><idno type="RBID">pubmed:25929564</idno><idno type="pmid">25929564</idno><idno type="wicri:Area/PubMed/Corpus">000085</idno><idno type="wicri:explorRef" wicri:stream="PubMed" wicri:step="Corpus" wicri:corpus="PubMed">000085</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en">Mesoporous silica-coated upconversion nanocrystals for near infrared light-triggered control of gene expression in zebrafish.</title><author><name sortKey="Jayakumar, Muthu Kumara Gnanasammandhan" sort="Jayakumar, Muthu Kumara Gnanasammandhan" uniqKey="Jayakumar M" first="Muthu Kumara Gnanasammandhan" last="Jayakumar">Muthu Kumara Gnanasammandhan Jayakumar</name><affiliation><nlm:affiliation>Department of Biomedical Engineering, Faculty of Engineering, Block EA #03-12, National University of Singapore, 9 Engineering Drive 1, 117576, Singapore.</nlm:affiliation></affiliation></author><author><name sortKey="Bansal, Akshaya" sort="Bansal, Akshaya" uniqKey="Bansal A" first="Akshaya" last="Bansal">Akshaya Bansal</name></author><author><name sortKey="Li, Bing Nan" sort="Li, Bing Nan" uniqKey="Li B" first="Bing Nan" last="Li">Bing Nan Li</name></author><author><name sortKey="Zhang, Yong" sort="Zhang, Yong" uniqKey="Zhang Y" first="Yong" last="Zhang">Yong Zhang</name></author></analytic><series><title level="j">Nanomedicine (London, England)</title><idno type="eISSN">1748-6963</idno><imprint><date when="2015" type="published">2015</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Animals</term><term>DNA (administration & dosage)</term><term>DNA (genetics)</term><term>Gene Knockdown Techniques (methods)</term><term>Gene Transfer Techniques</term><term>Infrared Rays</term><term>Morpholinos (administration & dosage)</term><term>Morpholinos (genetics)</term><term>Nanoparticles (chemistry)</term><term>Nanoparticles (ultrastructure)</term><term>Nanotechnology</term><term>Photosensitizing Agents (chemistry)</term><term>Plasmids (administration & dosage)</term><term>Plasmids (genetics)</term><term>Silicon Dioxide (chemistry)</term><term>Thulium (chemistry)</term><term>Ultraviolet Rays</term><term>Ytterbium (chemistry)</term><term>Yttrium (chemistry)</term><term>Zebrafish (embryology)</term><term>Zebrafish (genetics)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="administration & dosage" xml:lang="en"><term>DNA</term><term>Morpholinos</term></keywords><keywords scheme="MESH" type="chemical" qualifier="chemistry" xml:lang="en"><term>Photosensitizing Agents</term><term>Silicon Dioxide</term><term>Thulium</term><term>Ytterbium</term><term>Yttrium</term></keywords><keywords scheme="MESH" type="chemical" qualifier="genetics" xml:lang="en"><term>DNA</term><term>Morpholinos</term></keywords><keywords scheme="MESH" qualifier="administration & dosage" xml:lang="en"><term>Plasmids</term></keywords><keywords scheme="MESH" qualifier="chemistry" xml:lang="en"><term>Nanoparticles</term></keywords><keywords scheme="MESH" qualifier="embryology" xml:lang="en"><term>Zebrafish</term></keywords><keywords scheme="MESH" qualifier="genetics" xml:lang="en"><term>Plasmids</term><term>Zebrafish</term></keywords><keywords scheme="MESH" qualifier="methods" xml:lang="en"><term>Gene Knockdown Techniques</term></keywords><keywords scheme="MESH" qualifier="ultrastructure" xml:lang="en"><term>Nanoparticles</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Animals</term><term>Gene Transfer Techniques</term><term>Infrared Rays</term><term>Nanotechnology</term><term>Ultraviolet Rays</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">To develop a platform technology for photoactivation of gene expression in deep tissues.</div></front></TEI><pubmed><MedlineCitation Owner="NLM" Status="MEDLINE"><PMID Version="1">25929564</PMID><DateCreated><Year>2015</Year><Month>05</Month><Day>01</Day></DateCreated><DateCompleted><Year>2016</Year><Month>01</Month><Day>25</Day></DateCompleted><Article PubModel="Print"><Journal><ISSN IssnType="Electronic">1748-6963</ISSN><JournalIssue CitedMedium="Internet"><Volume>10</Volume><Issue>7</Issue><PubDate><Year>2015</Year></PubDate></JournalIssue><Title>Nanomedicine (London, England)</Title><ISOAbbreviation>Nanomedicine (Lond)</ISOAbbreviation></Journal><ArticleTitle>Mesoporous silica-coated upconversion nanocrystals for near infrared light-triggered control of gene expression in zebrafish.</ArticleTitle><Pagination><MedlinePgn>1051-61</MedlinePgn></Pagination><ELocationID EIdType="doi" ValidYN="Y">10.2217/nnm.14.198</ELocationID><Abstract><AbstractText Label="AIM" NlmCategory="OBJECTIVE">To develop a platform technology for photoactivation of gene expression in deep tissues.</AbstractText><AbstractText Label="MATERIALS & METHODS" NlmCategory="METHODS">Upconversion nanoparticles (UCNs) were synthesized from rare earth elements like Ytterbium, Yttrium and Thulium. The nanoparticles were then further coated with a layer of mesoporous silica and loaded with photomorpholinos or photocaged plasmids and tested in zebrafish. The UCNs were activated using safe near-infrared (NIR) light which in turn produced UV light locally to enable photoactivation in deep tissues.</AbstractText><AbstractText Label="RESULTS" NlmCategory="RESULTS">Light-controlled gene knockdown was demonstrated in an in vivo model, namely zebrafish. UCNs loaded with photomorpholinos were used to knockdown a gene - ntl, which is essential for notochord formation and mesoderm patterning in zebrafish using NIR light. UCN-mediated light-controlled gene expression was also achieved by expressing GFP in tumor cells transplanted into adult zebrafish by irradiating the fish with NIR light. Apart from the delivery and control of genes, the UCNs were also used as imaging agents to image both zebrafish embryos and adult zebrafish. enabled excellent background-free, fluorescent imaging of both embryos and adult zebrafish.</AbstractText><AbstractText Label="CONCLUSION" NlmCategory="CONCLUSIONS">This technique of controlling gene expression/knockdown through NIR using UCNs is a game changer in the field of genetic manipulation and has the potential of being an excellent, safe and easy to implement tool for developmental biologists to investigate the role of specific genes in development. However, this technique is not restricted to be used only in zebrafish and can be extended for use in other animal models and even for clinical use, in various gene therapy applications.</AbstractText></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Jayakumar</LastName><ForeName>Muthu Kumara Gnanasammandhan</ForeName><Initials>MK</Initials><AffiliationInfo><Affiliation>Department of Biomedical Engineering, Faculty of Engineering, Block EA #03-12, National University of Singapore, 9 Engineering Drive 1, 117576, Singapore.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Bansal</LastName><ForeName>Akshaya</ForeName><Initials>A</Initials></Author><Author ValidYN="Y"><LastName>Li</LastName><ForeName>Bing Nan</ForeName><Initials>BN</Initials></Author><Author ValidYN="Y"><LastName>Zhang</LastName><ForeName>Yong</ForeName><Initials>Y</Initials></Author></AuthorList><Language>eng</Language><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType><PublicationType UI="D013485">Research Support, Non-U.S. Gov't</PublicationType></PublicationTypeList></Article><MedlineJournalInfo><Country>England</Country><MedlineTA>Nanomedicine (Lond)</MedlineTA><NlmUniqueID>101278111</NlmUniqueID><ISSNLinking>1743-5889</ISSNLinking></MedlineJournalInfo><ChemicalList><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D060172">Morpholinos</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D017319">Photosensitizing Agents</NameOfSubstance></Chemical><Chemical><RegistryNumber>58784XQC3Y</RegistryNumber><NameOfSubstance UI="D015019">Yttrium</NameOfSubstance></Chemical><Chemical><RegistryNumber>7631-86-9</RegistryNumber><NameOfSubstance UI="D012822">Silicon Dioxide</NameOfSubstance></Chemical><Chemical><RegistryNumber>8RKC5ATI4P</RegistryNumber><NameOfSubstance UI="D013932">Thulium</NameOfSubstance></Chemical><Chemical><RegistryNumber>9007-49-2</RegistryNumber><NameOfSubstance UI="D004247">DNA</NameOfSubstance></Chemical><Chemical><RegistryNumber>MNQ4O4WSI1</RegistryNumber><NameOfSubstance UI="D015018">Ytterbium</NameOfSubstance></Chemical></ChemicalList><CitationSubset>IM</CitationSubset><MeshHeadingList><MeshHeading><DescriptorName MajorTopicYN="N" UI="D000818">Animals</DescriptorName></MeshHeading><MeshHeading><DescriptorName MajorTopicYN="N" UI="D004247">DNA</DescriptorName><QualifierName MajorTopicYN="N" UI="Q000008">administration & dosage</QualifierName><QualifierName MajorTopicYN="N" UI="Q000235">genetics</QualifierName></MeshHeading><MeshHeading><DescriptorName MajorTopicYN="N" UI="D055785">Gene Knockdown Techniques</DescriptorName><QualifierName MajorTopicYN="Y" UI="Q000379">methods</QualifierName></MeshHeading><MeshHeading><DescriptorName MajorTopicYN="N" UI="D018014">Gene Transfer Techniques</DescriptorName></MeshHeading><MeshHeading><DescriptorName MajorTopicYN="N" UI="D007259">Infrared Rays</DescriptorName></MeshHeading><MeshHeading><DescriptorName MajorTopicYN="N" UI="D060172">Morpholinos</DescriptorName><QualifierName MajorTopicYN="Y" UI="Q000008">administration & dosage</QualifierName><QualifierName MajorTopicYN="N" UI="Q000235">genetics</QualifierName></MeshHeading><MeshHeading><DescriptorName MajorTopicYN="N" UI="D053758">Nanoparticles</DescriptorName><QualifierName MajorTopicYN="Y" UI="Q000737">chemistry</QualifierName><QualifierName MajorTopicYN="N" UI="Q000648">ultrastructure</QualifierName></MeshHeading><MeshHeading><DescriptorName MajorTopicYN="N" UI="D036103">Nanotechnology</DescriptorName></MeshHeading><MeshHeading><DescriptorName MajorTopicYN="N" UI="D017319">Photosensitizing Agents</DescriptorName><QualifierName MajorTopicYN="Y" UI="Q000737">chemistry</QualifierName></MeshHeading><MeshHeading><DescriptorName MajorTopicYN="N" UI="D010957">Plasmids</DescriptorName><QualifierName MajorTopicYN="Y" UI="Q000008">administration & dosage</QualifierName><QualifierName MajorTopicYN="N" UI="Q000235">genetics</QualifierName></MeshHeading><MeshHeading><DescriptorName MajorTopicYN="N" UI="D012822">Silicon Dioxide</DescriptorName><QualifierName MajorTopicYN="Y" UI="Q000737">chemistry</QualifierName></MeshHeading><MeshHeading><DescriptorName MajorTopicYN="N" UI="D013932">Thulium</DescriptorName><QualifierName MajorTopicYN="N" UI="Q000737">chemistry</QualifierName></MeshHeading><MeshHeading><DescriptorName MajorTopicYN="N" UI="D014466">Ultraviolet Rays</DescriptorName></MeshHeading><MeshHeading><DescriptorName MajorTopicYN="N" UI="D015018">Ytterbium</DescriptorName><QualifierName MajorTopicYN="N" UI="Q000737">chemistry</QualifierName></MeshHeading><MeshHeading><DescriptorName MajorTopicYN="N" UI="D015019">Yttrium</DescriptorName><QualifierName MajorTopicYN="N" UI="Q000737">chemistry</QualifierName></MeshHeading><MeshHeading><DescriptorName MajorTopicYN="N" UI="D015027">Zebrafish</DescriptorName><QualifierName MajorTopicYN="N" UI="Q000196">embryology</QualifierName><QualifierName MajorTopicYN="Y" UI="Q000235">genetics</QualifierName></MeshHeading></MeshHeadingList><KeywordList Owner="NOTNLM"><Keyword MajorTopicYN="N">nanoparticle</Keyword><Keyword MajorTopicYN="N">photoactivation</Keyword><Keyword MajorTopicYN="N">photomorpholino</Keyword><Keyword MajorTopicYN="N">upconversion</Keyword><Keyword MajorTopicYN="N">zebrafish</Keyword></KeywordList></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="entrez"><Year>2015</Year><Month>5</Month><Day>2</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="pubmed"><Year>2015</Year><Month>5</Month><Day>2</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="medline"><Year>2016</Year><Month>1</Month><Day>26</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate></History><PublicationStatus>ppublish</PublicationStatus><ArticleIdList><ArticleId IdType="doi">10.2217/nnm.14.198</ArticleId><ArticleId IdType="pubmed">25929564</ArticleId></ArticleIdList></PubmedData></pubmed></record>Pour manipuler ce document sous Unix (Dilib)
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