Quantitative Imaging of Single Upconversion Nanoparticles in Biological Tissue
Identifieur interne : 000388 ( Pmc/Curation ); précédent : 000387; suivant : 000389Quantitative Imaging of Single Upconversion Nanoparticles in Biological Tissue
Auteurs : Annemarie Nadort [Australie, Pays-Bas] ; Varun K. A. Sreenivasan [Australie] ; Zhen Song [Australie] ; Ekaterina A. Grebenik [Australie, Russie] ; Andrei V. Nechaev [Russie] ; Vladimir A. Semchishen [Russie] ; Vladislav Y. Panchenko [Russie] ; Andrei V. Zvyagin [Australie, Russie]Source :
- PLoS ONE [ 1932-6203 ] ; 2013.
Abstract
The unique luminescent properties of new-generation synthetic nanomaterials, upconversion nanoparticles (UCNPs), enabled high-contrast optical biomedical imaging by suppressing the crowded background of biological tissue autofluorescence and evading high tissue absorption. This raised high expectations on the UCNP utilities for intracellular and deep tissue imaging, such as whole animal imaging. At the same time, the critical nonlinear dependence of the UCNP luminescence on the excitation intensity results in dramatic signal reduction at (∼1 cm) depth in biological tissue. Here, we report on the experimental and theoretical investigation of this trade-off aiming at the identification of optimal application niches of UCNPs e.g. biological liquids and subsurface tissue layers. As an example of such applications, we report on single UCNP imaging through a layer of hemolyzed blood. To extend this result towards
Url:
DOI: 10.1371/journal.pone.0063292
PubMed: 23691012
PubMed Central: 3653952
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A." last="Sreenivasan">Varun K. A. Sreenivasan</name><affiliation wicri:level="1"><nlm:aff id="aff1"><addr-line>MQ Biofocus Research Centre, Macquarie University, Sydney, NSW, Australia</addr-line></nlm:aff><country xml:lang="fr">Australie</country><wicri:regionArea>MQ Biofocus Research Centre, Macquarie University, Sydney, NSW</wicri:regionArea></affiliation></author><author><name sortKey="Song, Zhen" sort="Song, Zhen" uniqKey="Song Z" first="Zhen" last="Song">Zhen Song</name><affiliation wicri:level="1"><nlm:aff id="aff1"><addr-line>MQ Biofocus Research Centre, Macquarie University, Sydney, NSW, Australia</addr-line></nlm:aff><country xml:lang="fr">Australie</country><wicri:regionArea>MQ Biofocus Research Centre, Macquarie University, Sydney, NSW</wicri:regionArea></affiliation></author><author><name sortKey="Grebenik, Ekaterina A" sort="Grebenik, Ekaterina A" uniqKey="Grebenik E" first="Ekaterina A." last="Grebenik">Ekaterina A. Grebenik</name><affiliation wicri:level="1"><nlm:aff id="aff1"><addr-line>MQ Biofocus Research Centre, Macquarie University, Sydney, NSW, Australia</addr-line></nlm:aff><country xml:lang="fr">Australie</country><wicri:regionArea>MQ Biofocus Research Centre, Macquarie University, Sydney, NSW</wicri:regionArea></affiliation><affiliation wicri:level="1"><nlm:aff id="aff3"><addr-line>Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, Moscow, Russia</addr-line></nlm:aff><country xml:lang="fr">Russie</country><wicri:regionArea>Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, Moscow</wicri:regionArea></affiliation></author><author><name sortKey="Nechaev, Andrei V" sort="Nechaev, Andrei V" uniqKey="Nechaev A" first="Andrei V." last="Nechaev">Andrei V. Nechaev</name><affiliation wicri:level="1"><nlm:aff id="aff4"><addr-line>HTBAS Department, Lomonosov Moscow State University of Fine Chemical Technologies, Moscow, Russia</addr-line></nlm:aff><country xml:lang="fr">Russie</country><wicri:regionArea>HTBAS Department, Lomonosov Moscow State University of Fine Chemical Technologies, Moscow</wicri:regionArea></affiliation></author><author><name sortKey="Semchishen, Vladimir A" sort="Semchishen, Vladimir A" uniqKey="Semchishen V" first="Vladimir A." last="Semchishen">Vladimir A. Semchishen</name><affiliation wicri:level="1"><nlm:aff id="aff5"><addr-line>Institute of Laser and Information Technology, Russian Academy of Sciences, Troitsk, Moscow Region, Russia</addr-line></nlm:aff><country xml:lang="fr">Russie</country><wicri:regionArea>Institute of Laser and Information Technology, Russian Academy of Sciences, Troitsk, Moscow Region</wicri:regionArea></affiliation></author><author><name sortKey="Panchenko, Vladislav Y" sort="Panchenko, Vladislav Y" uniqKey="Panchenko V" first="Vladislav Y." last="Panchenko">Vladislav Y. Panchenko</name><affiliation wicri:level="1"><nlm:aff id="aff5"><addr-line>Institute of Laser and Information Technology, Russian Academy of Sciences, Troitsk, Moscow Region, Russia</addr-line></nlm:aff><country xml:lang="fr">Russie</country><wicri:regionArea>Institute of Laser and Information Technology, Russian Academy of Sciences, Troitsk, Moscow Region</wicri:regionArea></affiliation></author><author><name sortKey="Zvyagin, Andrei V" sort="Zvyagin, Andrei V" uniqKey="Zvyagin A" first="Andrei V." last="Zvyagin">Andrei V. Zvyagin</name><affiliation wicri:level="1"><nlm:aff id="aff1"><addr-line>MQ Biofocus Research Centre, Macquarie University, Sydney, NSW, Australia</addr-line></nlm:aff><country xml:lang="fr">Australie</country><wicri:regionArea>MQ Biofocus Research Centre, Macquarie University, Sydney, NSW</wicri:regionArea></affiliation><affiliation wicri:level="1"><nlm:aff id="aff5"><addr-line>Institute of Laser and Information Technology, Russian Academy of Sciences, Troitsk, Moscow Region, Russia</addr-line></nlm:aff><country xml:lang="fr">Russie</country><wicri:regionArea>Institute of Laser and Information Technology, Russian Academy of Sciences, Troitsk, Moscow Region</wicri:regionArea></affiliation></author></titleStmt><publicationStmt><idno type="wicri:source">PMC</idno><idno type="pmid">23691012</idno><idno type="pmc">3653952</idno><idno type="url">http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3653952</idno><idno type="RBID">PMC:3653952</idno><idno type="doi">10.1371/journal.pone.0063292</idno><date when="2013">2013</date><idno type="wicri:Area/Pmc/Corpus">000388</idno><idno type="wicri:explorRef" wicri:stream="Pmc" wicri:step="Corpus" wicri:corpus="PMC">000388</idno><idno type="wicri:Area/Pmc/Curation">000388</idno><idno type="wicri:explorRef" wicri:stream="Pmc" wicri:step="Curation">000388</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en" level="a" type="main">Quantitative Imaging of Single Upconversion Nanoparticles in Biological Tissue</title><author><name sortKey="Nadort, Annemarie" sort="Nadort, Annemarie" uniqKey="Nadort A" first="Annemarie" last="Nadort">Annemarie Nadort</name><affiliation wicri:level="1"><nlm:aff id="aff1"><addr-line>MQ Biofocus Research Centre, Macquarie University, Sydney, NSW, Australia</addr-line></nlm:aff><country xml:lang="fr">Australie</country><wicri:regionArea>MQ Biofocus Research Centre, Macquarie University, Sydney, NSW</wicri:regionArea></affiliation><affiliation wicri:level="1"><nlm:aff id="aff2"><addr-line>Department of Biomedical Engineering and Physics, Academic Medical Center, University of Amsterdam, Amsterdam, The Netherlands</addr-line></nlm:aff><country xml:lang="fr">Pays-Bas</country><wicri:regionArea>Department of Biomedical Engineering and Physics, Academic Medical Center, University of Amsterdam, Amsterdam</wicri:regionArea></affiliation></author><author><name sortKey="Sreenivasan, Varun K A" sort="Sreenivasan, Varun K A" uniqKey="Sreenivasan V" first="Varun K. A." last="Sreenivasan">Varun K. A. Sreenivasan</name><affiliation wicri:level="1"><nlm:aff id="aff1"><addr-line>MQ Biofocus Research Centre, Macquarie University, Sydney, NSW, Australia</addr-line></nlm:aff><country xml:lang="fr">Australie</country><wicri:regionArea>MQ Biofocus Research Centre, Macquarie University, Sydney, NSW</wicri:regionArea></affiliation></author><author><name sortKey="Song, Zhen" sort="Song, Zhen" uniqKey="Song Z" first="Zhen" last="Song">Zhen Song</name><affiliation wicri:level="1"><nlm:aff id="aff1"><addr-line>MQ Biofocus Research Centre, Macquarie University, Sydney, NSW, Australia</addr-line></nlm:aff><country xml:lang="fr">Australie</country><wicri:regionArea>MQ Biofocus Research Centre, Macquarie University, Sydney, NSW</wicri:regionArea></affiliation></author><author><name sortKey="Grebenik, Ekaterina A" sort="Grebenik, Ekaterina A" uniqKey="Grebenik E" first="Ekaterina A." last="Grebenik">Ekaterina A. Grebenik</name><affiliation wicri:level="1"><nlm:aff id="aff1"><addr-line>MQ Biofocus Research Centre, Macquarie University, Sydney, NSW, Australia</addr-line></nlm:aff><country xml:lang="fr">Australie</country><wicri:regionArea>MQ Biofocus Research Centre, Macquarie University, Sydney, NSW</wicri:regionArea></affiliation><affiliation wicri:level="1"><nlm:aff id="aff3"><addr-line>Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, Moscow, Russia</addr-line></nlm:aff><country xml:lang="fr">Russie</country><wicri:regionArea>Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, Moscow</wicri:regionArea></affiliation></author><author><name sortKey="Nechaev, Andrei V" sort="Nechaev, Andrei V" uniqKey="Nechaev A" first="Andrei V." last="Nechaev">Andrei V. Nechaev</name><affiliation wicri:level="1"><nlm:aff id="aff4"><addr-line>HTBAS Department, Lomonosov Moscow State University of Fine Chemical Technologies, Moscow, Russia</addr-line></nlm:aff><country xml:lang="fr">Russie</country><wicri:regionArea>HTBAS Department, Lomonosov Moscow State University of Fine Chemical Technologies, Moscow</wicri:regionArea></affiliation></author><author><name sortKey="Semchishen, Vladimir A" sort="Semchishen, Vladimir A" uniqKey="Semchishen V" first="Vladimir A." last="Semchishen">Vladimir A. Semchishen</name><affiliation wicri:level="1"><nlm:aff id="aff5"><addr-line>Institute of Laser and Information Technology, Russian Academy of Sciences, Troitsk, Moscow Region, Russia</addr-line></nlm:aff><country xml:lang="fr">Russie</country><wicri:regionArea>Institute of Laser and Information Technology, Russian Academy of Sciences, Troitsk, Moscow Region</wicri:regionArea></affiliation></author><author><name sortKey="Panchenko, Vladislav Y" sort="Panchenko, Vladislav Y" uniqKey="Panchenko V" first="Vladislav Y." last="Panchenko">Vladislav Y. Panchenko</name><affiliation wicri:level="1"><nlm:aff id="aff5"><addr-line>Institute of Laser and Information Technology, Russian Academy of Sciences, Troitsk, Moscow Region, Russia</addr-line></nlm:aff><country xml:lang="fr">Russie</country><wicri:regionArea>Institute of Laser and Information Technology, Russian Academy of Sciences, Troitsk, Moscow Region</wicri:regionArea></affiliation></author><author><name sortKey="Zvyagin, Andrei V" sort="Zvyagin, Andrei V" uniqKey="Zvyagin A" first="Andrei V." last="Zvyagin">Andrei V. Zvyagin</name><affiliation wicri:level="1"><nlm:aff id="aff1"><addr-line>MQ Biofocus Research Centre, Macquarie University, Sydney, NSW, Australia</addr-line></nlm:aff><country xml:lang="fr">Australie</country><wicri:regionArea>MQ Biofocus Research Centre, Macquarie University, Sydney, NSW</wicri:regionArea></affiliation><affiliation wicri:level="1"><nlm:aff id="aff5"><addr-line>Institute of Laser and Information Technology, Russian Academy of Sciences, Troitsk, Moscow Region, Russia</addr-line></nlm:aff><country xml:lang="fr">Russie</country><wicri:regionArea>Institute of Laser and Information Technology, Russian Academy of Sciences, Troitsk, Moscow Region</wicri:regionArea></affiliation></author></analytic><series><title level="j">PLoS ONE</title><idno type="eISSN">1932-6203</idno><imprint><date when="2013">2013</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en"><p>The unique luminescent properties of new-generation synthetic nanomaterials, upconversion nanoparticles (UCNPs), enabled high-contrast optical biomedical imaging by suppressing the crowded background of biological tissue autofluorescence and evading high tissue absorption. This raised high expectations on the UCNP utilities for intracellular and deep tissue imaging, such as whole animal imaging. At the same time, the critical nonlinear dependence of the UCNP luminescence on the excitation intensity results in dramatic signal reduction at (∼1 cm) depth in biological tissue. Here, we report on the experimental and theoretical investigation of this trade-off aiming at the identification of optimal application niches of UCNPs e.g. biological liquids and subsurface tissue layers. As an example of such applications, we report on single UCNP imaging through a layer of hemolyzed blood. To extend this result towards <italic>in vivo</italic> applications, we quantified the optical properties of single UCNPs and theoretically analyzed the prospects of single-particle detectability in live scattering and absorbing bio-tissue using a human skin model. The model predicts that a single 70-nm UCNP would be detectable at skin depths up to 400 µm, unlike a hardly detectable single fluorescent (fluorescein) dye molecule. UCNP-assisted imaging in the ballistic regime thus allows for excellent applications niches, where high sensitivity is the key requirement.</p></div></front><back><div1 type="bibliography"><listBibl><biblStruct><analytic><author><name sortKey="Hilderbrand, Sa" uniqKey="Hilderbrand S">SA Hilderbrand</name></author><author><name sortKey="Weissleder, R" uniqKey="Weissleder R">R Weissleder</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Patterson, Ms" uniqKey="Patterson M">MS Patterson</name></author><author><name sortKey="Chance, B" uniqKey="Chance B">B Chance</name></author><author><name sortKey="Wilson, Bc" uniqKey="Wilson B">BC Wilson</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Corlu, A" uniqKey="Corlu A">A Corlu</name></author><author><name sortKey="Choe, R" uniqKey="Choe R">R Choe</name></author><author><name sortKey="Durduran, T" uniqKey="Durduran T">T Durduran</name></author><author><name sortKey="Rosen, 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<front><journal-meta><journal-id journal-id-type="nlm-ta">PLoS One</journal-id><journal-id journal-id-type="iso-abbrev">PLoS ONE</journal-id><journal-id journal-id-type="publisher-id">plos</journal-id><journal-id journal-id-type="pmc">plosone</journal-id><journal-title-group><journal-title>PLoS ONE</journal-title></journal-title-group><issn pub-type="epub">1932-6203</issn><publisher><publisher-name>Public Library of Science</publisher-name><publisher-loc>San Francisco, USA</publisher-loc></publisher></journal-meta><article-meta><article-id pub-id-type="pmid">23691012</article-id><article-id pub-id-type="pmc">3653952</article-id><article-id pub-id-type="publisher-id">PONE-D-12-39172</article-id><article-id pub-id-type="doi">10.1371/journal.pone.0063292</article-id><article-categories><subj-group subj-group-type="heading"><subject>Research Article</subject></subj-group><subj-group subj-group-type="Discipline-v2"><subject>Biology</subject><subj-group><subject>Biotechnology</subject><subj-group><subject>Bionanotechnology</subject></subj-group></subj-group></subj-group><subj-group subj-group-type="Discipline-v2"><subject>Engineering</subject><subj-group><subject>Bioengineering</subject><subj-group><subject>Biomedical Engineering</subject></subj-group></subj-group><subj-group><subject>Signal Processing</subject><subj-group><subject>Image Processing</subject></subj-group></subj-group></subj-group><subj-group subj-group-type="Discipline-v2"><subject>Materials Science</subject><subj-group><subject>Material by Attribute</subject><subj-group><subject>Nanomaterials</subject></subj-group></subj-group><subj-group><subject>Nanotechnology</subject><subj-group><subject>Bionanotechnology</subject><subject>Nanomaterials</subject></subj-group></subj-group></subj-group><subj-group subj-group-type="Discipline-v2"><subject>Physics</subject><subj-group><subject>Condensed-Matter Physics</subject><subj-group><subject>Optics</subject></subj-group></subj-group></subj-group></article-categories><title-group><article-title>Quantitative Imaging of Single Upconversion Nanoparticles in Biological Tissue</article-title><alt-title alt-title-type="running-head">Bio-Imaging of Single Upconversion Nanoparticles</alt-title></title-group><contrib-group><contrib contrib-type="author"><name><surname>Nadort</surname><given-names>Annemarie</given-names></name><xref ref-type="aff" rid="aff1"><sup>1</sup></xref><xref ref-type="aff" rid="aff2"><sup>2</sup></xref></contrib><contrib contrib-type="author"><name><surname>Sreenivasan</surname><given-names>Varun K. A.</given-names></name><xref ref-type="aff" rid="aff1"><sup>1</sup></xref></contrib><contrib contrib-type="author"><name><surname>Song</surname><given-names>Zhen</given-names></name><xref ref-type="aff" rid="aff1"><sup>1</sup></xref></contrib><contrib contrib-type="author"><name><surname>Grebenik</surname><given-names>Ekaterina A.</given-names></name><xref ref-type="aff" rid="aff1"><sup>1</sup></xref><xref ref-type="aff" rid="aff3"><sup>3</sup></xref></contrib><contrib contrib-type="author"><name><surname>Nechaev</surname><given-names>Andrei V.</given-names></name><xref ref-type="aff" rid="aff4"><sup>4</sup></xref></contrib><contrib contrib-type="author"><name><surname>Semchishen</surname><given-names>Vladimir A.</given-names></name><xref ref-type="aff" rid="aff5"><sup>5</sup></xref></contrib><contrib contrib-type="author"><name><surname>Panchenko</surname><given-names>Vladislav Y.</given-names></name><xref ref-type="aff" rid="aff5"><sup>5</sup></xref></contrib><contrib contrib-type="author"><name><surname>Zvyagin</surname><given-names>Andrei V.</given-names></name><xref ref-type="aff" rid="aff1"><sup>1</sup></xref><xref ref-type="aff" rid="aff5"><sup>5</sup></xref><xref ref-type="corresp" rid="cor1"><sup>*</sup></xref></contrib></contrib-group><aff id="aff1"><label>1</label><addr-line>MQ Biofocus Research Centre, Macquarie University, Sydney, NSW, Australia</addr-line></aff><aff id="aff2"><label>2</label><addr-line>Department of Biomedical Engineering and Physics, Academic Medical Center, University of Amsterdam, Amsterdam, The Netherlands</addr-line></aff><aff id="aff3"><label>3</label><addr-line>Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, Moscow, Russia</addr-line></aff><aff id="aff4"><label>4</label><addr-line>HTBAS Department, Lomonosov Moscow State University of Fine Chemical Technologies, Moscow, Russia</addr-line></aff><aff id="aff5"><label>5</label><addr-line>Institute of Laser and Information Technology, Russian Academy of Sciences, Troitsk, Moscow Region, Russia</addr-line></aff><contrib-group><contrib contrib-type="editor"><name><surname>Shankar</surname><given-names>Sangaru Shiv</given-names></name><role>Editor</role><xref ref-type="aff" rid="edit1"></xref></contrib></contrib-group><aff id="edit1"><addr-line>King Abdullah University of Science and Technology, Saudi Arabia</addr-line></aff><author-notes><corresp id="cor1">* E-mail: <email>andrei.zvyagin@mq.edu.au</email></corresp><fn fn-type="conflict"><p><bold>Competing Interests: </bold>The authors have declared that no competing interests exist.</p></fn><fn fn-type="con"><p>Conceived and designed the experiments: AN VKAS ZS EAG VAS VYP AVZ. Performed the experiments: AN VKAS ZS EAG AVN VAS. Analyzed the data: AN VKAS EAG AVZ. Contributed reagents/materials/analysis tools: AN VKAS ZS EAG AVN VAS. Wrote the paper: AN VKAS ZS AVN VAS VYP AVZ.</p></fn></author-notes><pub-date pub-type="collection"><year>2013</year></pub-date><pub-date pub-type="epub"><day>14</day><month>5</month><year>2013</year></pub-date><volume>8</volume><issue>5</issue><elocation-id>e63292</elocation-id><history><date date-type="received"><day>11</day><month>12</month><year>2012</year></date><date date-type="accepted"><day>29</day><month>3</month><year>2013</year></date></history><permissions><copyright-year>2013</copyright-year><copyright-holder>Nadort et al</copyright-holder><license><license-p>This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.</license-p></license></permissions><abstract><p>The unique luminescent properties of new-generation synthetic nanomaterials, upconversion nanoparticles (UCNPs), enabled high-contrast optical biomedical imaging by suppressing the crowded background of biological tissue autofluorescence and evading high tissue absorption. This raised high expectations on the UCNP utilities for intracellular and deep tissue imaging, such as whole animal imaging. At the same time, the critical nonlinear dependence of the UCNP luminescence on the excitation intensity results in dramatic signal reduction at (∼1 cm) depth in biological tissue. Here, we report on the experimental and theoretical investigation of this trade-off aiming at the identification of optimal application niches of UCNPs e.g. biological liquids and subsurface tissue layers. As an example of such applications, we report on single UCNP imaging through a layer of hemolyzed blood. To extend this result towards <italic>in vivo</italic> applications, we quantified the optical properties of single UCNPs and theoretically analyzed the prospects of single-particle detectability in live scattering and absorbing bio-tissue using a human skin model. The model predicts that a single 70-nm UCNP would be detectable at skin depths up to 400 µm, unlike a hardly detectable single fluorescent (fluorescein) dye molecule. UCNP-assisted imaging in the ballistic regime thus allows for excellent applications niches, where high sensitivity is the key requirement.</p></abstract><funding-group><funding-statement>The authors thankfully acknowledge the financial support by the Russian Foundation of Basic Research #11-04-12113, Russia, the Prins Bernhard Cultuur fonds (The Netherlands), and PGRF Macquarie University, Australia. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.</funding-statement></funding-group><counts><page-count count="13"></page-count></counts></article-meta></front></pmc></record>Pour manipuler ce document sous Unix (Dilib)
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