Novel interfaces for light directed neuronal stimulation: advances and challenges
Identifieur interne : 000418 ( Pmc/Corpus ); précédent : 000417; suivant : 000419Novel interfaces for light directed neuronal stimulation: advances and challenges
Auteurs : Lilach Bareket-Keren ; Yael HaneinSource :
- International Journal of Nanomedicine [ 1176-9114 ] ; 2014.
Abstract
Light activation of neurons is a growing field with applications ranging from basic investigation of neuronal systems to the development of new therapeutic methods such as artificial retina. Many recent studies currently explore novel methods for optical stimulation with temporal and spatial precision. Novel materials in particular provide an opportunity to enhance contemporary approaches. Here we review recent advances towards light directed interfaces for neuronal stimulation, focusing on state-of-the-art nanoengineered devices. In particular, we highlight challenges and prospects towards improved retinal prostheses.
Url:
DOI: 10.2147/IJN.S51193
PubMed: 24872704
PubMed Central: 4024977
Links to Exploration step
PMC:4024977Le document en format XML
<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">Novel interfaces for light directed neuronal stimulation: advances and challenges</title><author><name sortKey="Bareket Keren, Lilach" sort="Bareket Keren, Lilach" uniqKey="Bareket Keren L" first="Lilach" last="Bareket-Keren">Lilach Bareket-Keren</name><affiliation><nlm:aff id="af1-ijn-9-065">School of Electrical Engineering, Tel-Aviv University, Tel-Aviv University, Tel-Aviv, Israel</nlm:aff></affiliation><affiliation><nlm:aff id="af2-ijn-9-065">Tel-Aviv University Center for Nanoscience and Nanotechnology, Tel-Aviv University, Tel-Aviv, Israel</nlm:aff></affiliation></author><author><name sortKey="Hanein, Yael" sort="Hanein, Yael" uniqKey="Hanein Y" first="Yael" last="Hanein">Yael Hanein</name><affiliation><nlm:aff id="af1-ijn-9-065">School of Electrical Engineering, Tel-Aviv University, Tel-Aviv University, Tel-Aviv, Israel</nlm:aff></affiliation><affiliation><nlm:aff id="af2-ijn-9-065">Tel-Aviv University Center for Nanoscience and Nanotechnology, Tel-Aviv University, Tel-Aviv, Israel</nlm:aff></affiliation></author></titleStmt><publicationStmt><idno type="wicri:source">PMC</idno><idno type="pmid">24872704</idno><idno type="pmc">4024977</idno><idno type="url">http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4024977</idno><idno type="RBID">PMC:4024977</idno><idno type="doi">10.2147/IJN.S51193</idno><date when="2014">2014</date><idno type="wicri:Area/Pmc/Corpus">000418</idno><idno type="wicri:explorRef" wicri:stream="Pmc" wicri:step="Corpus" wicri:corpus="PMC">000418</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en" level="a" type="main">Novel interfaces for light directed neuronal stimulation: advances and challenges</title><author><name sortKey="Bareket Keren, Lilach" sort="Bareket Keren, Lilach" uniqKey="Bareket Keren L" first="Lilach" last="Bareket-Keren">Lilach Bareket-Keren</name><affiliation><nlm:aff id="af1-ijn-9-065">School of Electrical Engineering, Tel-Aviv University, Tel-Aviv University, Tel-Aviv, Israel</nlm:aff></affiliation><affiliation><nlm:aff id="af2-ijn-9-065">Tel-Aviv University Center for Nanoscience and Nanotechnology, Tel-Aviv University, Tel-Aviv, Israel</nlm:aff></affiliation></author><author><name sortKey="Hanein, Yael" sort="Hanein, Yael" uniqKey="Hanein Y" first="Yael" last="Hanein">Yael Hanein</name><affiliation><nlm:aff id="af1-ijn-9-065">School of Electrical Engineering, Tel-Aviv University, Tel-Aviv University, Tel-Aviv, Israel</nlm:aff></affiliation><affiliation><nlm:aff id="af2-ijn-9-065">Tel-Aviv University Center for Nanoscience and Nanotechnology, Tel-Aviv University, Tel-Aviv, Israel</nlm:aff></affiliation></author></analytic><series><title level="j">International Journal of Nanomedicine</title><idno type="ISSN">1176-9114</idno><idno type="eISSN">1178-2013</idno><imprint><date when="2014">2014</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en"><p>Light activation of neurons is a growing field with applications ranging from basic investigation of neuronal systems to the development of new therapeutic methods such as artificial retina. Many recent studies currently explore novel methods for optical stimulation with temporal and spatial precision. Novel materials in particular provide an opportunity to enhance contemporary approaches. Here we review recent advances towards light directed interfaces for neuronal stimulation, focusing on state-of-the-art nanoengineered devices. 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<front><journal-meta><journal-id journal-id-type="nlm-ta">Int J Nanomedicine</journal-id><journal-id journal-id-type="iso-abbrev">Int J Nanomedicine</journal-id><journal-title-group><journal-title>International Journal of Nanomedicine</journal-title></journal-title-group><issn pub-type="ppub">1176-9114</issn><issn pub-type="epub">1178-2013</issn><publisher><publisher-name>Dove Medical Press</publisher-name></publisher></journal-meta><article-meta><article-id pub-id-type="pmid">24872704</article-id><article-id pub-id-type="pmc">4024977</article-id><article-id pub-id-type="doi">10.2147/IJN.S51193</article-id><article-id pub-id-type="publisher-id">ijn-9-065</article-id><article-categories><subj-group subj-group-type="heading"><subject>Review</subject></subj-group></article-categories><title-group><article-title>Novel interfaces for light directed neuronal stimulation: advances and challenges</article-title></title-group><contrib-group><contrib contrib-type="author"><name><surname>Bareket-Keren</surname><given-names>Lilach</given-names></name><xref ref-type="aff" rid="af1-ijn-9-065">1</xref><xref ref-type="aff" rid="af2-ijn-9-065">2</xref></contrib><contrib contrib-type="author"><name><surname>Hanein</surname><given-names>Yael</given-names></name><xref ref-type="aff" rid="af1-ijn-9-065">1</xref><xref ref-type="aff" rid="af2-ijn-9-065">2</xref><xref ref-type="corresp" rid="c1-ijn-9-065"></xref></contrib></contrib-group><aff id="af1-ijn-9-065"><label>1</label>School of Electrical Engineering, Tel-Aviv University, Tel-Aviv University, Tel-Aviv, Israel</aff><aff id="af2-ijn-9-065"><label>2</label>Tel-Aviv University Center for Nanoscience and Nanotechnology, Tel-Aviv University, Tel-Aviv, Israel</aff><author-notes><corresp id="c1-ijn-9-065">Correspondence: Yael Hanein, Tel-Aviv University Ramat-Aviv, Tel-Aviv 69978, Israel, Email <email>yaelha@tauex.tau.ac.il</email></corresp></author-notes><pub-date pub-type="collection"><year>2014</year></pub-date><pub-date pub-type="epub"><day>06</day><month>5</month><year>2014</year></pub-date><volume>9</volume><issue>Suppl 1</issue><fpage>65</fpage><lpage>83</lpage><permissions><copyright-statement>© 2014 Bareket-Keren and Hanein. This work is published by Dove Medical Press Limited, and licensed under Creative Commons Attribution – Non Commercial (unported, v3.0) License</copyright-statement><copyright-year>2014</copyright-year><license><license-p>The full terms of the License are available at <ext-link ext-link-type="uri" xlink:href="http://creativecommons.org/licenses/by-nc/3.0/">http://creativecommons.org/licenses/by-nc/3.0/</ext-link>. Non-commercial uses of the work are permitted without any further permission from Dove Medical Press Limited, provided the work is properly attributed.</license-p></license></permissions><abstract><p>Light activation of neurons is a growing field with applications ranging from basic investigation of neuronal systems to the development of new therapeutic methods such as artificial retina. Many recent studies currently explore novel methods for optical stimulation with temporal and spatial precision. Novel materials in particular provide an opportunity to enhance contemporary approaches. Here we review recent advances towards light directed interfaces for neuronal stimulation, focusing on state-of-the-art nanoengineered devices. In particular, we highlight challenges and prospects towards improved retinal prostheses.</p></abstract><kwd-group><title>Keywords</title><kwd>neuronal stimulation</kwd><kwd>light</kwd><kwd>retinal implant</kwd><kwd>prosthesis</kwd><kwd>quantum dots</kwd><kwd>conducting polymers</kwd><kwd>photoconductive</kwd></kwd-group></article-meta></front><body><sec sec-type="intro"><title>Introduction</title><p>Impaired connectivity, or dysfunctional elements in the neural system, can hinder cell activation and proper information transfer. Accordingly, artificial devices capable of locally stimulating neurons are used to treat conditions such as chronic pain, Parkinson’s disease, and deafness.<xref rid="b1-ijn-9-065" ref-type="bibr">1</xref>–<xref rid="b3-ijn-9-065" ref-type="bibr">3</xref> Neurostimulation is currently studied for the treatment of a broader range of neural impairments including psychiatric disorders, Alzheimer’s disease, restoration of motor activity, and blindness due to retinal degeneration.<xref rid="b4-ijn-9-065" ref-type="bibr">4</xref>–<xref rid="b6-ijn-9-065" ref-type="bibr">6</xref></p><p>Activation of neurons in these applications commonly employs electrical stimulation using extra cellular electrodes, and intensive investigations were dedicated over the years to improve the electrical interfacing between electrodes and neuronal tissues. Despite ongoing efforts, these technologies show major limitations. In particular, extracellular electrodes are typified by relatively low spatial resolution<xref rid="b7-ijn-9-065" ref-type="bibr">7</xref>,<xref rid="b8-ijn-9-065" ref-type="bibr">8</xref> and limited biocompatibility,<xref rid="b9-ijn-9-065" ref-type="bibr">9</xref>,<xref rid="b10-ijn-9-065" ref-type="bibr">10</xref> owing to large device dimensions and the need for cumbersome wiring.</p><p>An alternative approach to achieve neuronal activation is through light directed stimulation. Optical methods for stimulating neuronal activity offer many advantages compared with electrical technologies. Light can be easily focused to a fine spatial resolution allowing flexibility in targeting single neurons and even discrete regions of a single neuron.<xref rid="b11-ijn-9-065" ref-type="bibr">11</xref> When used within safe limits, light as a stimulation tool allows temporally and spatially precise activation of neurons, both in vitro and in vivo application,<xref rid="b12-ijn-9-065" ref-type="bibr">12</xref>,<xref rid="b13-ijn-9-065" ref-type="bibr">13</xref> and can be used in biomedical applications such as drug screening, basic neuroscience investigations and neural implants, in particular for retinal implant applications.</p><p>The aim of this review is to explore the progress achieved to date in harnessing novel materials and nanomaterials for the activation of neurons, in particular towards developing a retinal prosthesis. Commonly employed routes for neuronal optical stimulation, including genetic and chemical approaches, as well as the use of infrared (IR) light, will be briefly mentioned. We will focus on recent investigations and novel strategies utilizing thin film photoactive interfaces for light stimulation of neurons and specifically for future application in vision restoration. Finally, we will discuss challenges and prospects towards a biocompatible, nanomaterial-based technology for light stimulation of neural tissue.</p></sec><sec><title>Contemporary retinal implants</title><p>We begin by reviewing contemporary retinal implant technology. Retinal implants are of particular interest in the realm of light directed stimulation, owing to the fact that the retina is readily accessed from outside the body by light. Accordingly, direct light activation has immense potential in this field.</p><p>Retinal prostheses aim to restore vision following blindness caused by degenerative diseases such as retinitis pigmentosa (RP),<xref rid="b14-ijn-9-065" ref-type="bibr">14</xref> and age-related macular degeneration (AMD).<xref rid="b15-ijn-9-065" ref-type="bibr">15</xref></p><p>In RP and related diseases, the photoreceptive apparatus of the retina degenerates, resulting in retinal inability to transmit light-evoked responses to the visual cortex, despite the integrity of the output cells of the retina, the ganglion cells.<xref rid="b16-ijn-9-065" ref-type="bibr">16</xref>,<xref rid="b17-ijn-9-065" ref-type="bibr">17</xref> In such impairments, the majority of the physiological system is still operational and artificial stimulation can be envisioned as a means to circumvent the broken elements. It has long been suggested that an artificial system capable of locally stimulating remaining neurons in degenerated retinas, will allow vision restoration.<xref rid="b18-ijn-9-065" ref-type="bibr">18</xref>–<xref rid="b20-ijn-9-065" ref-type="bibr">20</xref></p><p>Until recently, efforts in this field relied mostly on conventional microfabrication technology<xref rid="b6-ijn-9-065" ref-type="bibr">6</xref>,<xref rid="b21-ijn-9-065" ref-type="bibr">21</xref>,<xref rid="b22-ijn-9-065" ref-type="bibr">22</xref> and combining metal electrodes with a light sensitive element is a well-established stimulation strategy already used in several retinal prostheses. In these devices, light is absorbed and transduced into a pattern of electrical pulses which stimulate the neural tissue. Two main schemes exist utilizing electrode arrays and photo sensors as retinal prosthesis for vision restoration. In the first, an external device (such as a small camera) captures the light and a microprocessor is used to convert the light to electrical signals.<xref rid="b23-ijn-9-065" ref-type="bibr">23</xref>–<xref rid="b26-ijn-9-065" ref-type="bibr">26</xref> A second approach incorporates a photodiode array with the stimulating electrodes on the same device.<xref rid="b13-ijn-9-065" ref-type="bibr">13</xref>,<xref rid="b27-ijn-9-065" ref-type="bibr">27</xref>–<xref rid="b29-ijn-9-065" ref-type="bibr">29</xref> The electrode array is positioned either on the surface of the retina (epiretinal) to interface with the retinal ganglion cells (RGCs), or under the retina (subretinal) to substitute for the degenerated photoreceptors and to stimulate the inner retinal neurons (mostly bipolar, but possibly also amacrine and horizontal cells).</p><p>When an external light detection system is used, the photoactive apparatus does not interface with neural cells. Light sensing, electrical signal generation, and neuronal stimulation functionalities operate at different elements of the device. Prosthetic devices, which have been implanted in humans, include the devices developed by Second Sight Medical Products Inc (Argus I and Argus II; Sylmar, CA, USA),<xref rid="b22-ijn-9-065" ref-type="bibr">22</xref>,<xref rid="b26-ijn-9-065" ref-type="bibr">26</xref>,<xref rid="b30-ijn-9-065" ref-type="bibr">30</xref> EpiRet GmBH (EpiRet3; Giessen, Germany),<xref rid="b25-ijn-9-065" ref-type="bibr">25</xref> Retina Implant AG (Reutlingen, Germany),<xref rid="b23-ijn-9-065" ref-type="bibr">23</xref> Intelligent Medical Implants GmBH (Bonn, Germany),<xref rid="b31-ijn-9-065" ref-type="bibr">31</xref> and Fujikado et al (Osaka University, Osaka, Japan).<xref rid="b24-ijn-9-065" ref-type="bibr">24</xref> The epiretinal Argus II device contains a 60-electrode array and an external processing unit which is wired through a cut in the sclera. An external camera captures the light and a wireless transmitter is used to transfer information from the camera to the processing unit.<xref rid="b22-ijn-9-065" ref-type="bibr">22</xref>,<xref rid="b26-ijn-9-065" ref-type="bibr">26</xref>,<xref rid="b30-ijn-9-065" ref-type="bibr">30</xref> The epiretinal EpiRet3 device includes 25 electrodes and a system designed to be completely implanted within the eye without the need for any cabling to pass through the sclera.<xref rid="b25-ijn-9-065" ref-type="bibr">25</xref> Patients implanted with such devices experience improved visual function, and with some patients who are implanted with the Argus II prosthesis, letters can be identified.<xref rid="b21-ijn-9-065" ref-type="bibr">21</xref></p><p>An alternative to the external camera is the integration of photodiodes and stimulating electrodes on a single unit. This integrated electrode-photodiode array represents the first step towards a photoactive surface for neural stimulation. Both light transduction and electrical triggering functionalities are placed on the same device. The device may be implanted in the location of the lost photoreceptors (subretinally), and no wiring to an external device is needed.</p><p>The first generation of photodiode based prostheses suffered from two problems. First, the silicon photodiodes did not develop strong enough voltage to evoke neural stimulation with ambient illumination. Second, continuous illumination resulted with polarization of the electrode-retinal tissue interface. Pulsed illumination,<xref rid="b32-ijn-9-065" ref-type="bibr">32</xref>,<xref rid="b33-ijn-9-065" ref-type="bibr">33</xref> extra power supply to amplify the photodiode signals,<xref rid="b13-ijn-9-065" ref-type="bibr">13</xref> or chaining several photodiodes,<xref rid="b34-ijn-9-065" ref-type="bibr">34</xref> have been explored to overcome these limitations.</p><p>Optobionics Corporation (Glen Ellyn, IL, USA), with the “artificial silicon retina” (ASR),<xref rid="b29-ijn-9-065" ref-type="bibr">29</xref>,<xref rid="b35-ijn-9-065" ref-type="bibr">35</xref> and Retina Implant AG<xref rid="b13-ijn-9-065" ref-type="bibr">13</xref> have already implanted subretinal microphotodiode-based implants in humans. The ASR consists of 5,000 microphotodiodes with stimulating electrodes at each cell.<xref rid="b35-ijn-9-065" ref-type="bibr">35</xref> Patients reported visual perception from regions well away from where the device was located, suggesting that light detection may also result from induced indirect stimulation of the remaining retina. The Retina Implant AG device consists of 1,500 microphotodiodes, each integrated with an amplifier and a stimulating electrode. Patients implanted with this device reported an improvement in light detection with some patients being able to recognize objects and read letters.<xref rid="b13-ijn-9-065" ref-type="bibr">13</xref></p><p>Follow up reports on subjects with implants are available and describe the long-term performances of these devices. Subjects implanted with the Argus II for 6 months and up to 2.7 years performed statistically better in visual tasks. Moreover, the long-term safety of the system was found to be acceptable. Chow et al<xref rid="b29-ijn-9-065" ref-type="bibr">29</xref> reported that the ASR device implanted in six patients was well-tolerated, and improvement and/or slowing of vision loss occurred in all patients. Visual percepts elicited reliably in eight out of eleven patients implanted with the Retina Implant device.<xref rid="b23-ijn-9-065" ref-type="bibr">23</xref> Activation of single-electrode or pattern of electrodes resulted with percepts described as round spots of light of distinguishable localization in the visual field or pattern of spots, respectively.<xref rid="b23-ijn-9-065" ref-type="bibr">23</xref></p><p>These pioneering investigations of photoactive neuronal interfaces, employing chiefly top-down fabrication methods, have so far achieved encouraging results and are currently the only approach capable of restoring vision. Notwithstanding these promising results, contemporary retinal prostheses suffer from limited spatial resolution and still require a connection to processors and to a power supply. Furthermore, the use of silicon based technology raises concerns about biocompatibility and stability issues.<xref rid="b36-ijn-9-065" ref-type="bibr">36</xref>,<xref rid="b37-ijn-9-065" ref-type="bibr">37</xref> The large difference between the mechanical properties of the electrodes and the device contributes to mechanical stress in addition to the damage caused during surgical manipulation, which may result in a severe immune response.<xref rid="b36-ijn-9-065" ref-type="bibr">36</xref>–<xref rid="b40-ijn-9-065" ref-type="bibr">40</xref> Damage, and even failure, of the device can also be caused by the highly corrosive biological environment.<xref rid="b38-ijn-9-065" ref-type="bibr">38</xref>,<xref rid="b41-ijn-9-065" ref-type="bibr">41</xref> Challenges notwithstanding, direct light activation remains a promising approach to achieve high density stimulation with simple chip architecture. Although contemporary light directed neuronal stimulation approaches also suffer from various limitations, such as the need to apply voltage bias in photoconductive silicon surfaces or the need to locate IR laser sources in close proximity to neural tissue (as will be discussed later in the text), recent progress in material science and nanotechnology, opened exciting routes towards photostimulation of neurons.</p></sec><sec><title>Light directed stimulation of neurons</title><p>Four main routes are commonly used to optically activate neurons. The first is through genetic manipulation of neurons, making them photosensitive by introducing light sensitive membrane proteins to neuronal cells (optogenetics).<xref rid="b42-ijn-9-065" ref-type="bibr">42</xref>–<xref rid="b44-ijn-9-065" ref-type="bibr">44</xref> The second is by applying caged neurotransmitters.<xref rid="b11-ijn-9-065" ref-type="bibr">11</xref>,<xref rid="b45-ijn-9-065" ref-type="bibr">45</xref> The third is photothermal neural stimulation by illuminating the neural tissue using an IR laser;<xref rid="b46-ijn-9-065" ref-type="bibr">46</xref> the fourth approach uses devices capable of developing an electrical signal in response to light. Neuronal electrical activity is then commonly monitored by intracellular or extracellular electrodes<xref rid="b47-ijn-9-065" ref-type="bibr">47</xref>,<xref rid="b48-ijn-9-065" ref-type="bibr">48</xref> or calcium imaging.<xref rid="b11-ijn-9-065" ref-type="bibr">11</xref> Our main focus in this review is the fourth category, but for clarity we begin our review with a brief description of all four strategies.</p><sec><title>Optogenetics</title><p>Neurons can become light sensitive by genetic methods, causing them to express photosensitive proteins. These photosensitive proteins act as photo-switches, allowing the control of neuronal activity with light.<xref rid="b42-ijn-9-065" ref-type="bibr">42</xref>,<xref rid="b49-ijn-9-065" ref-type="bibr">49</xref> The most common optogenetic method is the introduction of bacterial opsins into neurons through viral transfection.<xref rid="b50-ijn-9-065" ref-type="bibr">50</xref> Prime examples are channelrhodopsin, which acts as a nonspecific cation channel,<xref rid="b51-ijn-9-065" ref-type="bibr">51</xref>,<xref rid="b52-ijn-9-065" ref-type="bibr">52</xref> bacteriorhodopsin, which acts as a proton pump,<xref rid="b53-ijn-9-065" ref-type="bibr">53</xref> or halorhodopsin, which acts as a chloride ion pump.<xref rid="b51-ijn-9-065" ref-type="bibr">51</xref>,<xref rid="b54-ijn-9-065" ref-type="bibr">54</xref> Restricting gene expression to specific neuronal cell types offers a selective photostimulation.</p><p>Optogenetics was studied for the application of vision restoration,<xref rid="b43-ijn-9-065" ref-type="bibr">43</xref> and a recent study demonstrated the use of a holographic system for patterned light stimulation of mice retina with millisecond temporal precision and cellular resolution.<xref rid="b55-ijn-9-065" ref-type="bibr">55</xref> Generating photoactivitated neurons in specific parts of brains of freely moving animals was studied as a means to achieve controlled behavioral response.<xref rid="b56-ijn-9-065" ref-type="bibr">56</xref> Illumination of genetically modified neurons in drosophila flies resulted in complex behaviors, such as jumping, wing beating, and flight, specific to the functionality of the brain area where neuronal modification was performed.<xref rid="b44-ijn-9-065" ref-type="bibr">44</xref>,<xref rid="b45-ijn-9-065" ref-type="bibr">45</xref> Optogenetics can therefore allow for the study of neural circuits.<xref rid="b57-ijn-9-065" ref-type="bibr">57</xref>,<xref rid="b58-ijn-9-065" ref-type="bibr">58</xref> Although very promising, there are many challenges that have to be overcome to make this technology suitable for vision restoration. Foremost is the long-term expression of light sensitive proteins and possible adverse immune system responses.</p></sec><sec><title>Caged glutamate</title><p>Another approach to achieve light sensitivity in neurons is through photochemical stimulation by caged neurotransmitter molecules.<xref rid="b59-ijn-9-065" ref-type="bibr">59</xref> A neurotransmitter is initially caged by a chemical group, which can be removed by light. An increase in neurotransmitter concentration, at the illuminated spot, excites neurons. Glutamate is the most commonly used caged neurotransmitter owing to its ubiquitous role,<xref rid="b60-ijn-9-065" ref-type="bibr">60</xref>,<xref rid="b61-ijn-9-065" ref-type="bibr">61</xref> and is commonly uncaged by ultraviolet light pulses.<xref rid="b59-ijn-9-065" ref-type="bibr">59</xref>,<xref rid="b62-ijn-9-065" ref-type="bibr">62</xref> Due to light scattering in living tissue, single-photon excitation results in multiple neuron stimulation.<xref rid="b11-ijn-9-065" ref-type="bibr">11</xref> Higher spatial resolution can be achieved by two-photon microscopy.<xref rid="b11-ijn-9-065" ref-type="bibr">11</xref>,<xref rid="b47-ijn-9-065" ref-type="bibr">47</xref>,<xref rid="b63-ijn-9-065" ref-type="bibr">63</xref>,<xref rid="b64-ijn-9-065" ref-type="bibr">64</xref></p><p>Recent developments in this field include new visible light-sensitive caged glutamate,<xref rid="b65-ijn-9-065" ref-type="bibr">65</xref> as well as caged versions of other neurotransmitters, including gamma-aminobutyric acid (GABA)<xref rid="b66-ijn-9-065" ref-type="bibr">66</xref>,<xref rid="b67-ijn-9-065" ref-type="bibr">67</xref> and glycine.<xref rid="b68-ijn-9-065" ref-type="bibr">68</xref>,<xref rid="b69-ijn-9-065" ref-type="bibr">69</xref> Furthermore, it has recently been reported that intraocular injection of a photo-switchable probe transiently restores light sensitivity in mouse models of RP.<xref rid="b70-ijn-9-065" ref-type="bibr">70</xref> Although photochemical stimulation can be used with fine spatial resolution, it has low temporal precision since the active neurotransmitters may stay active for a long period after release,<xref rid="b59-ijn-9-065" ref-type="bibr">59</xref> and may not be useful for repetitive stimulation if the receptors desensitize.<xref rid="b71-ijn-9-065" ref-type="bibr">71</xref></p><p>Spontaneous hydrolysis can also cause accumulation of the active compound which may lead to toxic effects and receptor desensitization.<xref rid="b72-ijn-9-065" ref-type="bibr">72</xref> In addition, selectivity is poor since common transmitters act on many neuronal cell types.<xref rid="b73-ijn-9-065" ref-type="bibr">73</xref> Finally, this photochemical approach has a major disadvantage as a therapy for vision restoration due to the need for repeated injections of the caged neurotransmitters. For further reading on optogenetics and caged neurotransmitters we refer the reader to Fenno et al,<xref rid="b74-ijn-9-065" ref-type="bibr">74</xref> Kramer et al,<xref rid="b45-ijn-9-065" ref-type="bibr">45</xref> Rogan and Roth,<xref rid="b75-ijn-9-065" ref-type="bibr">75</xref> and Szobota and Isacoff.<xref rid="b12-ijn-9-065" ref-type="bibr">12</xref></p></sec><sec><title>IR light</title><p>IR laser has been employed for neural stimulation. The dominant interaction between the IR light and the neural tissue is light absorption by water. The application of IR light for neural stimulation targets a restricted volume of tissue, with penetration depths of 100 to 1,500 μm. Thus, the stimulation source (optical fiber) is placed in close proximity to the neurons.</p><p>Wells et al<xref rid="b76-ijn-9-065" ref-type="bibr">76</xref>,<xref rid="b77-ijn-9-065" ref-type="bibr">77</xref> pioneered the use of pulsed IR laser for in vivo stimulation of the sciatic nerve of frogs and rats employing safe stimulation conditions of 2.1 μm and 0.65 J/cm<sup>2</sup>.<xref rid="b77-ijn-9-065" ref-type="bibr">77</xref> Recent studies have further demonstrated the effectiveness of IR light neural stimulation for in vivo stimulation of peripheral nerves,<xref rid="b78-ijn-9-065" ref-type="bibr">78</xref>–<xref rid="b80-ijn-9-065" ref-type="bibr">80</xref> facial nerve,<xref rid="b81-ijn-9-065" ref-type="bibr">81</xref> auditory nerve,<xref rid="b82-ijn-9-065" ref-type="bibr">82</xref>–<xref rid="b84-ijn-9-065" ref-type="bibr">84</xref> vestibular nerves,<xref rid="b85-ijn-9-065" ref-type="bibr">85</xref> and the cochlea.<xref rid="b86-ijn-9-065" ref-type="bibr">86</xref> Stimulation of in vitro preparations was demonstrated as well.<xref rid="b87-ijn-9-065" ref-type="bibr">87</xref>,<xref rid="b88-ijn-9-065" ref-type="bibr">88</xref></p><p>The mechanism for IR neural photostimulation is not entirely understood to date and includes photothermal, photomechanical, and photochemical processes. As concluded by Wells et al,<xref rid="b89-ijn-9-065" ref-type="bibr">89</xref> neuronal activation using IR light occurs mainly by a photothermal mechanism due to light-induced temperature transients. These thermal transients are thought to activate ion channels, or alternatively thermally induce biophysical or physical (expansion or pore formation) changes in the cell membrane.<xref rid="b46-ijn-9-065" ref-type="bibr">46</xref> Part of IR neural stimulation may also occur through photochemical and photomechanical effects.<xref rid="b46-ijn-9-065" ref-type="bibr">46</xref>,<xref rid="b89-ijn-9-065" ref-type="bibr">89</xref></p><p>The foremost advantage of using pulsed IR laser for neural stimulation is the potential to achieve high spatial resolution. Only neural tissue that is directly in the optical path will be stimulated. Furthermore, there is no electrochemical junction between the tissue and the stimulation source, which is a major failure point for electrical stimulation devices.<xref rid="b46-ijn-9-065" ref-type="bibr">46</xref>,<xref rid="b90-ijn-9-065" ref-type="bibr">90</xref></p><p>Limitations of this approach include potential thermal tissue damage. In addition, scattering from the optical path may decrease the anticipated spatial resolution. A technical drawback is the realization of high-density neural prostheses, as 50–100 individually addressed optical fibers are difficult to implement. Further investigation of the stimulation mechanism and additional in vitro preparations are required to optimize of this technique.<xref rid="b46-ijn-9-065" ref-type="bibr">46</xref>,<xref rid="b90-ijn-9-065" ref-type="bibr">90</xref></p></sec></sec><sec><title>Photoactive surfaces</title><p>An alternative route to chemical, genetic, and thermal approaches is by employing extracellular electrodes with light sensitivity. Recent pioneering investigations have proposed novel strategies to achieve neuronal activation with light using thin film photosensitive electrode coatings. In the next sections, we will review these technologies, including harnessing the photoconductivity of silicon, the use of conducting polymers (CPs), and utilizing quantum dot (QD) based approaches.</p><sec><title>Photoconductive silicon</title><p>Photoconductivity of silicon can be harnessed to achieve light induced activation of neurons. When a potential difference is applied to the silicon–electrolyte interface, the silicon surface becomes depleted of majority charge carriers (see <xref ref-type="fig" rid="f1-ijn-9-065">Figure 1</xref>). Local illumination of silicon modulates conductivity in the depletion layer by generating free charge carriers, resulting in a transient stimulation effect at the illumination spot.<xref rid="b91-ijn-9-065" ref-type="bibr">91</xref> This photoconductive effect turns the silicon substrate into a light controlled electrode. For p-type silicon, electrons, which are the minority charge carriers, generate photocurrent upon application of reversed bias. Neuronal stimulation, based on this effect, was demonstrated by several groups utilizing either single crystal,<xref rid="b95-ijn-9-065" ref-type="bibr">95</xref>–<xref rid="b97-ijn-9-065" ref-type="bibr">97</xref> (<xref ref-type="fig" rid="f1-ijn-9-065">Figure 1A</xref> and <xref ref-type="fig" rid="f1-ijn-9-065">B</xref>) or hydrogenated amorphous silicon (a-Si:H)<xref rid="b98-ijn-9-065" ref-type="bibr">98</xref>–<xref rid="b101-ijn-9-065" ref-type="bibr">101</xref> (<xref ref-type="fig" rid="f1-ijn-9-065">Figure 1C</xref> and <xref ref-type="fig" rid="f1-ijn-9-065">1D</xref>).</p><p>Colicos et al were the first to demonstrate this approach.<xref rid="b92-ijn-9-065" ref-type="bibr">92</xref> Rat hippocampal cells were cultured on an untreated silicon surface immersed in a physiological solution. Stimulation was achieved by targeting a neuron cell body with a constant illumination and modulating the bias voltage between the silicon (which was connected to a base electrode) and an external platinum counter electrode,<xref rid="b92-ijn-9-065" ref-type="bibr">92</xref> as illustrated in <xref ref-type="fig" rid="f1-ijn-9-065">Figure 1A</xref>. Illumination, combined with voltage pulses (2 ms, 4 V), stimulated exclusively selected neurons. Stimulation was monitored by changes in the intracellular Ca<sup>+2</sup> by a membrane permeable Ca<sup>+2</sup> indicator dye. Formation of new synaptic connections in response to specific activity patterns were documented using this technology.</p><p>A similar photoconductive stimulation scheme, using single crystal silicon as a photoconducting substrate, was adopted by Starovoytov et al<xref rid="b94-ijn-9-065" ref-type="bibr">94</xref> and is illustrated in <xref ref-type="fig" rid="f1-ijn-9-065">Figure 1B</xref>. Here, stimulation is achieved by holding a fixed voltage between the p-type silicon and an Ag/AgCl counter electrode and modulating the laser beam illumination. The silicon wafer was cut into 1.5 cm by 1.5 cm square pieces. The unpolished back side of the silicon wafer was scratched, painted with InGa eutectic, and placed on a flat copper electrode, which was glued using silicone rubber to the bottom side of a petri dish.</p><p>Hippocampal neuron activity was monitored by intracellular recordings using a patch-clamp recording. Action potentials could be stimulated with 0.3 mW and 100 μs illumination. Threshold bias values for different neurons, were found to vary over a wide range (−0.4 to −1.5V), and only particular locations on putative axons were susceptible to stimulation. In addition, recorded responses were sometimes characterized by exactly the same latency, even when stimulated over a rather wide area. These effects were explained by electrical current leakage through low-resistivity cross-interface pathways; ie, microscopic holes in the glial layer, or non-uniformities in the semiconductor surface and/or the native oxide. It was hypothesized that most of the electric current escapes through such pathways found in 100-μm disks around the illuminated location, effecting only the pathways traversed by the neurites of the patched cells.<xref rid="b94-ijn-9-065" ref-type="bibr">94</xref></p><p>The temporal and spatial resolution of silicon photoexcitation is limited by minority charge carrier lifetime and diffusion coefficient, which is approximately equal to the thickness of the silicon.<xref rid="b99-ijn-9-065" ref-type="bibr">99</xref> It was argued that this resolution can be improved by patterning the silicon surface, and thinning the chip.<xref rid="b94-ijn-9-065" ref-type="bibr">94</xref>,<xref rid="b100-ijn-9-065" ref-type="bibr">100</xref> Another possibility is using low conductivity silicon which can be achieved, for example, by doping the silicon with gold. The recombination centers should reduce the lateral diffusion of photogenerated minority carriers.<xref rid="b101-ijn-9-065" ref-type="bibr">101</xref>,<xref rid="b102-ijn-9-065" ref-type="bibr">102</xref></p><p>a-Si:H was also employed as a photoconductive layer for neuronal activation. A scheme demonstrating neuronal light stimulation using an a-Si:H-based device is presented in <xref ref-type="fig" rid="f1-ijn-9-065">Figure 1D</xref>. a-Si:H can be thinned to improve the spatial resolution and permit optical access from the top and bottom.<xref rid="b97-ijn-9-065" ref-type="bibr">97</xref>,<xref rid="b103-ijn-9-065" ref-type="bibr">103</xref> However, owing to its poor stability in the physiological solution, it requires a passivation layer.<xref rid="b97-ijn-9-065" ref-type="bibr">97</xref> Light addressed recordings of cardiac myocytes, using an array of titanium nitride (TiN) microelectrodes on an a-Si:H photoconductive layer, was reported by Bucher et al.<xref rid="b96-ijn-9-065" ref-type="bibr">96</xref>,<xref rid="b104-ijn-9-065" ref-type="bibr">104</xref> An a-Si:H layer was sandwiched between indium tin oxide (ITO) leads on a glass substrate, and a layer of microelectrodes embedded in carbonic silicon oxide (SiOx:C) insulation as demonstrated in <xref ref-type="fig" rid="f1-ijn-9-065">Figure 1C</xref>. The spontaneous activity of cardiac myocytes was recorded upon illumination of the photoconductor with a 488 nm laser beam with a spot diameter of 1.6 μm and intensity of 1.6 mW.<xref rid="b96-ijn-9-065" ref-type="bibr">96</xref>,<xref rid="b104-ijn-9-065" ref-type="bibr">104</xref></p><p>An alternative passivation layer composed of low conductivity zinc antimonite (ZnOSb<sub>2</sub>O<sub>5</sub>) was suggested by Suzurikawa et al.<xref rid="b97-ijn-9-065" ref-type="bibr">97</xref>,<xref rid="b98-ijn-9-065" ref-type="bibr">98</xref> An a-Si:H layer was sandwiched between a tin oxide (SnO<sub>2</sub>) coated glass substrate and a zinc antimonate passivation layer, as demonstrated in <xref ref-type="fig" rid="f1-ijn-9-065">Figure 1D</xref>. This waterproof, low-conductivity passivation layer prevents spreading currents along the plane.<xref rid="b98-ijn-9-065" ref-type="bibr">98</xref> Rat cortical cells were cultured on a photosensitive electrode, coated with poly-D-lysine and laminin for better adhesion. Neuronal activation was achieved using 470–495 nm light with intensity of 800 mW/cm<sup>2</sup>, and voltage pulses of 3 V for 1 ms, as verified using calcium imaging. In a subsequent study, the authors further improved the simultaneous light stimulation and the Ca<sup>+2</sup> imaging experimental system<xref rid="b97-ijn-9-065" ref-type="bibr">97</xref> by incorporation of a digital micro-mirror device to achieve patterned illumination for neuronal activation. Neuronal responses decreased dramatically at approximately 10 μm from the illumination border, suggesting spatial resolution of a single-cell.</p><p>Photoconductive neuronal stimulation technology is comparably simple and can be easily implemented to study neurons in vitro. However, long-term in vitro applications are a challenge due to heating of the tissue. Furthermore, the need to apply bias is a drawback when considering in vivo applications. Other drawbacks include the mechanical difference between the rigid silicon and soft tissue which compromises biocompatibility,<xref rid="b105-ijn-9-065" ref-type="bibr">105</xref> the limited stability of the device in a biological environment,<xref rid="b41-ijn-9-065" ref-type="bibr">41</xref> and the need to use high intensity illumination which may damage the cells,<xref rid="b106-ijn-9-065" ref-type="bibr">106</xref> or result in cell death due to reactive oxygen species (ROS).<xref rid="b107-ijn-9-065" ref-type="bibr">107</xref>,<xref rid="b108-ijn-9-065" ref-type="bibr">108</xref></p><p>In comparison, stimulation of normal and degenerate rat retinas was achieved using photovoltaic silicon with pulsed near IR light at intensities of 0.2–10 mW/mm<sup>2</sup> with pulse durations of 0.5–4.0 ms. Photovoltaic silicon cancels the need to apply a bias voltage.<xref rid="b34-ijn-9-065" ref-type="bibr">34</xref> We now turn to discuss the use of photo induced effects in CPs for neuronal stimulation.</p></sec><sec><title>CPs</title><p>CPs have been studied extensively in the past few decades for a variety of optoelectronic applications such as solar cells<xref rid="b112-ijn-9-065" ref-type="bibr">112</xref>,<xref rid="b113-ijn-9-065" ref-type="bibr">113</xref> and light emitting diodes.<xref rid="b111-ijn-9-065" ref-type="bibr">111</xref>,<xref rid="b112-ijn-9-065" ref-type="bibr">112</xref> When light is absorbed in organic materials, it results in a mobile excited state (excitons), rather than free electron–hole pairs as produced in inorganic materials. This occurs due to weak intermolecular forces and low dielectric permittivity which confines the exciton to a small volume (a few cubic nanometers)<xref rid="b113-ijn-9-065" ref-type="bibr">113</xref> and creates a large (0.1–1.0 eV) Coulombic barrier to their dissociation into separate electrons and holes.<xref rid="b114-ijn-9-065" ref-type="bibr">114</xref> Thus, instead of dissociating, the excitons diffuse within the organic layer until they reach an electrode. Exciton diffusion length (typically a few nanometers)<xref rid="b115-ijn-9-065" ref-type="bibr">115</xref> limits photocurrent generation because most excitons recombinant before reaching the electrode.<xref rid="b114-ijn-9-065" ref-type="bibr">114</xref>,<xref rid="b116-ijn-9-065" ref-type="bibr">116</xref></p><p>To establish efficient photocurrent generation, such devices are based on a heterojunction between an electron-donating and an electron-accepting material, which allows photogenerated excitons to dissociate. At the donor–acceptor interface exciton, dissociation is energetically favored over the exciton state of either donor or acceptor.<xref rid="b114-ijn-9-065" ref-type="bibr">114</xref> Various donor–acceptor pairs have been investigated so far, including polymer–fullerene,<xref rid="b117-ijn-9-065" ref-type="bibr">117</xref> polymer–perylene,<xref rid="b118-ijn-9-065" ref-type="bibr">118</xref>,<xref rid="b119-ijn-9-065" ref-type="bibr">119</xref> polymer–polymer,<xref rid="b120-ijn-9-065" ref-type="bibr">120</xref> and polymer–QD.<xref rid="b121-ijn-9-065" ref-type="bibr">121</xref>,<xref rid="b122-ijn-9-065" ref-type="bibr">122</xref></p><p>The two main designs of CP photovoltaic devices are planar and bulk heterojunction (BHJ). Planar heterojunction devices rely on the diffusion of excitons to the donor– acceptor interface to achieve charge separation.<xref rid="b123-ijn-9-065" ref-type="bibr">123</xref>,<xref rid="b124-ijn-9-065" ref-type="bibr">124</xref> The short exciton diffusion length (<10 nm), compared with the absorption depth of the film, limits the thickness of the effective light-harvesting layer. This limitation ultimately reduces the efficiency of photon to electron generation, and thus the effectiveness of photostimulation. BHJ devices are formed by blending the donor and the acceptor species together to achieve phase-segregated architecture.</p><p>The BHJ architecture widens the interface between donor and acceptor, maximizes the probability for charge pair separation, and dramatically increases photocurrent generation.<xref rid="b117-ijn-9-065" ref-type="bibr">117</xref>,<xref rid="b119-ijn-9-065" ref-type="bibr">119</xref> However, this continuous donor–acceptor structure increases the probability for charge recombination in the bulk,<xref rid="b123-ijn-9-065" ref-type="bibr">123</xref>,<xref rid="b124-ijn-9-065" ref-type="bibr">124</xref> which may reduce photocurrent generation and ultimately diminish the effectiveness of photostimulation.</p><p>Harnessing polymers for light stimulation of neurons is conditional for maintaining their photoelectric properties under biological conditions. In particular, oxygen and aqueous solutions may have a negative effect, such as degradation and quenching species. Examples for two photovoltaic polymeric mixtures, which were tested and found stable in aqueous solution conditions include: poly(3-octylthiophene): N2200 (P3OT:N2200)<xref rid="b125-ijn-9-065" ref-type="bibr">125</xref> and poly(3- hexylthiophene): phenyl-C61-butyric-acid-methyl ester (P3HT:PCBM).<xref rid="b126-ijn-9-065" ref-type="bibr">126</xref></p><p>Ghezzi et al<xref rid="b127-ijn-9-065" ref-type="bibr">127</xref> demonstrated photostimulation of neurons by a polymeric mixture-based scheme (P3HT:PCBM), illustrated in <xref ref-type="fig" rid="f2-ijn-9-065">Figure 2</xref>. In this BHJ structure, P3HT is the electron donor, whereas PCBM is the electron acceptor. To achieve the photoactive neuronal interface, an ITO coated glass substrate was spin-coated with the photovoltaic polymer blend, followed by a poly-L-lysine coating to improve cell adhesion. The patch-clamp technique was used to record the electrical activity of primary rat embryonic hippocampal neurons, cultured on the polymer coated surface. In addition, neuronal network activity was characterized by extracellular recording of primary rat embryonic hippocampal neurons, cultured on a polymer coated ITO microelectrode array.</p><p>It was found that neuronal viability and network development were not compromised by the P3HT:PCBM film as was demonstrated by cell staining tests. Furthermore, no differences in spontaneous activity and network firing were observed between cells cultured on the P3HT:PCBM coated surfaces and cells cultured on uncoated surfaces. Rapid neuronal excitation was evoked with short light pulses (532 nm) at an intensity of 10 mW/mm<sup>2</sup> for 20 ms. Capacitive coupling was suggested as the stimulation mechanism, as no adverse effects were observed on either the polymeric film or the neuronal culture, yet a parallel Faradaic component was not ruled out.</p><p>In a subsequent study by the same group, neuronal photostimulation by a photoactive interface composed only of the donor component (P3HT), was demonstrated<xref rid="b128-ijn-9-065" ref-type="bibr">128</xref> (similar to the scheme illustrated in <xref ref-type="fig" rid="f2-ijn-9-065">Figure 2</xref>, with the P3HT layer instead of the P3HT:PCBM layer). The use of pure P3HT may reduce material toxicity during light exposure, as fullerene photoexcitation has been reported to produce ROS.<xref rid="b129-ijn-9-065" ref-type="bibr">129</xref> An ITO substrate was spin-coated with the polymer, followed by poly-L-lysine coating. Viability staining assays and patch-clamp recordings were used to validate biocompatibility. Activation of primary rat hippocampal neurons cultured on a polymer coated substrate was accomplished by light pulses (532 nm, 20 ms, 15 mW/mm<sup>2</sup>). Trains of repetitive light pulses effectively triggered spiking activity up to an illumination frequency of 10 Hz. Higher frequencies resulted in some of the pulses failing to trigger neuronal stimulation.</p><p>Next, P3HT coated substrates were applied to activate retinas explanted from albino rats with light induced photoreceptor degeneration. Retinas were placed on the P3HT-coated ITO or on uncoated ITO in a subretinal configuration, and compared with healthy retinas. Activity was recorded with an extracellular electrode placed at the RGC layer. Light stimulation (10 ms, 4 mW/mm<sup>2</sup>) elicited spiking activity in degenerated retinas placed on P3HT compared with the activity recorded in control retinas. This response appeared with a typical latency of ~70–100 ms from the light pulse onset and was blocked by tetrodotoxin, a sodium channel blocker. The authors suggested response latency and the persistence of the local field potential indicates that a residual external cells layer, which may include photoreceptors, contributed to RGC firing in the degenerate retinas. These cells will not play a role in realistic conditions of a degenerated retina. Photostimulation threshold was 0.3 μW/mm<sup>2</sup> when retinas were placed over P3HT, compared with a threshold of 80 μW/mm<sup>2</sup> achieved with retinas placed on uncoated ITO samples. The authors suggested that the stimulation mechanism is charge separation occurring mainly at the interface with the ITO, with minor contributions from carriers generated in the bulk. The accumulation of negative charges at the illuminated polymer surface attracts positive ions from the electrolytic solution, triggering neuronal membrane depolarization.</p><p>Stimulation of neurons using CPs as a photoactive layer has many advantages. Foremost, the fabrication of the polymeric interface is simple, and deposition methods such as ink-jet printing, allow the realization of small geometrical patterns. The use of soft polymer coatings may also provide a mechanical buffer between a rigid electrode and the soft tissue.<xref rid="b130-ijn-9-065" ref-type="bibr">130</xref> A major disadvantage of CPs is their low stability under continuous stimulation, exposure to ultraviolet light, or exposure to heat. During repeated insertion or removal of counter ions, CPs undergo swelling and shrinkage that may gradually degrade their conducting properties.<xref rid="b131-ijn-9-065" ref-type="bibr">131</xref>,<xref rid="b132-ijn-9-065" ref-type="bibr">132</xref> Additionally, synthetic CPs are often fabricated using complex or toxic polymerization schemes that are not well suited for cell interfacing applications. These residues are often not easily removed. These issues have yet to be tested to fully explore the potential of these materials.</p></sec><sec><title>QDs</title><p>Semiconducting QDs are nanoparticles (NPs) with versatile absorbing and emitting properties that can be tuned by modifying their size, shape, and composition. Quantum confinement provides these particles with band gaps covering the ultraviolet-visual-near IR range. With high quantum efficiency, photostability, and biocompatibility, QDs are rapidly being applied in biological systems primarily as fluorescent labels in both in vitro and in vivo applications.<xref rid="b133-ijn-9-065" ref-type="bibr">133</xref>–<xref rid="b135-ijn-9-065" ref-type="bibr">135</xref> QDs are also used in biosensors,<xref rid="b136-ijn-9-065" ref-type="bibr">136</xref>–<xref rid="b138-ijn-9-065" ref-type="bibr">138</xref> cancer treatment,<xref rid="b139-ijn-9-065" ref-type="bibr">139</xref>,<xref rid="b140-ijn-9-065" ref-type="bibr">140</xref> and drug delivery.<xref rid="b141-ijn-9-065" ref-type="bibr">141</xref> Moreover, QDs display unique optoelectronic properties and can generate photocurrent.<xref rid="b142-ijn-9-065" ref-type="bibr">142</xref> Under some conditions, free electrons or holes can escape the QD confinement and create an electrical current. This property has motivated the use of QDs in photovoltaics, lasers, and light emitting diodes.<xref rid="b143-ijn-9-065" ref-type="bibr">143</xref>–<xref rid="b145-ijn-9-065" ref-type="bibr">145</xref></p><p>Optically excited electron-hole pairs in a QD generate a temporary electric dipole moment.<xref rid="b146-ijn-9-065" ref-type="bibr">146</xref> It was suggested that the electric field associated with this dipole may be strong enough to generate an action potential in neuronal cells.<xref rid="b147-ijn-9-065" ref-type="bibr">147</xref> Accordingly, QDs have significant potential in photo stimulation of neuronal cells. The quantum effects also offer the possibility to engineer the spectral sensitivity and electrical response in such QD devices.</p><p>Few pioneering studies have indicated the potential use of QDs in the realm of neuronal photo-activation. These studies demonstrated two main approaches to achieve light activation of neurons using QDs. The first approach is to directly wire QDs to the cell membrane,<xref rid="b147-ijn-9-065" ref-type="bibr">147</xref>,<xref rid="b148-ijn-9-065" ref-type="bibr">148</xref> as illustrated in <xref ref-type="fig" rid="f3-ijn-9-065">Figure 3A</xref>. Although this scheme has so far failed to result in neuronal photostimulation, the prospects of establishing a very short distance QD–neuron coupling (ie, tens of nanometers) is very promising. The second approach is to use a QD-coated surface in close proximity to neural cells, as illustrated in <xref ref-type="fig" rid="f3-ijn-9-065">Figure 3B</xref>–<xref ref-type="fig" rid="f3-ijn-9-065">D</xref>.</p><p>Formation of a tightly packed three-dimensional film of QDs should potentially result in strong electronic coupling between the photogenerated exciton, and may enhance the charge carrier separation and transport rate.<xref rid="b149-ijn-9-065" ref-type="bibr">149</xref> We review below these two approaches.</p><sec><title>Direct QD-neuron interface</title><p>Placing artificial chromophores, such as QDs at a close proximity to a cell membrane and its ion channels should potentially allow efficient optoelectrical interfacing. Illumination of QDs attached to a cell membrane induces charge separation. The electrical field associated with this separation of charge may activate the neuron, as illustrated in <xref ref-type="fig" rid="f3-ijn-9-065">Figure 3A</xref>. Direct QD–cell wiring should overcome several major challenges. First, large separation between the cell and a stimulating device leads to poor electrical coupling and low stimulation efficiency. Furthermore, it may provide the ability to target specific cells.</p><p>Direct binding of cadmium sulfide (CdS) QDs to neurons was achieved using biological recognition of neuronal cell surface receptors.<xref rid="b147-ijn-9-065" ref-type="bibr">147</xref>,<xref rid="b149-ijn-9-065" ref-type="bibr">149</xref> Two routes were demonstrated: antibody-antigen recognition and peptide recognition.<xref rid="b147-ijn-9-065" ref-type="bibr">147</xref> The antibody-antigen approach included a primary antibody binding to a specific cell surface receptor. Multiple secondary antibodies, attached to CdS QDs, bind to the primary antibody. QDs were coated with carboxylic groups to provide linking sites for amine-terminated antibodies. An alternative peptide approach included peptide recognition sequences binding to specific cell surface receptors. These sequences were embedded in the mercaptoacetic acid coating layer of the CdS QDs. The expected separation distance between the QDs and the cell for the indirect antibody-antigen attachment is ~30 nm, assuming no crosslinking of secondary antibodies and a linear attachment, while the direct peptide attachment separation distance is expected to be ~3 nm.<xref rid="b147-ijn-9-065" ref-type="bibr">147</xref> Both techniques were applied to attach CdS QDs to the SK-N-SH human neuronal cell line. The peptide conjugation technique resulted in reduced particle clustering compared to the antibody technique due to multiple secondary antibody binding and chemically induced cross-linking.</p><p>QD-neuron photoelectric interfaces face many challenges. One major challenge is the need to remove the thick surface coating of QDs. This thick coating, ie, polyethylene glycol<xref rid="b152-ijn-9-065" ref-type="bibr">152</xref> or bovine serum albumin,<xref rid="b153-ijn-9-065" ref-type="bibr">153</xref>,<xref rid="b154-ijn-9-065" ref-type="bibr">154</xref> used for bio-applications such as labeling and sensing, improves QD- protein targeting capability, biocompatibility, chemical stability, and photostability. However, to achieve an active cell–QD interface, these thick coatings prevent efficient charge transfer, leading to decreased electric field generation. In a subsequent study,<xref rid="b148-ijn-9-065" ref-type="bibr">148</xref> uncoated, mercaptoacetic acid-capped CdS and cadmium telluride (CdTe) QDs were added to three different nerve cell culture types: SK-N-SH neuronal cell line, PC12 neuronal cell line, and rat neonatal cortical cells. Multiple challenges were identified, starting from cytotoxicity of NPs and nonspecific binding, to rapid endocytosis of the attached NPs inside the cell. To establish a direct QD–neuron interface other solutions should be considered. For example, limiting the number of polyethylene glycol molecules, which constitute the surface coating, and developing new hydrophilic organic ligands, thereby producing a thin and tightly-packed shell. Another approach to diminish cytotoxic effects is by anchoring QDs to a device. This way QDs in contact with cells are neither in a dispersed state nor bound to the cell membrane, thus reducing the probability for endocytosis.</p></sec><sec><title>QD nanostructured interface</title><p>Several recent studies have demonstrated neuronal photoactivation using QD-based coatings. These surfaces may provide a solution to the biocompatibility challenge mentioned above.</p><p>Since QDs are attached to a surface, there is less chance for cellular penetration. Recently, drop casting,<xref rid="b150-ijn-9-065" ref-type="bibr">150</xref> as well as electrostatic layer-by-layer (LBL)<xref rid="b149-ijn-9-065" ref-type="bibr">149</xref>,<xref rid="b155-ijn-9-065" ref-type="bibr">155</xref> fabricated QD films were successfully used to achieve photostimulation of cultured neurons.</p><p>A mercury telluride (HgTe) QD layer on ITO was used for light activation of cultured neurons by Pappas et al.<xref rid="b149-ijn-9-065" ref-type="bibr">149</xref> The QD film was prepared using an electrostatic LBL method by layering negatively charged QDs, and positively charged poly(dimethyl-diallyl-ammonium-chloride) (PDDA) on an ITO substrate, as illustrated in <xref ref-type="fig" rid="f3-ijn-9-065">Figure 3A</xref>. To improve the biocompatibility of the QD coated surface, it was coated with polylysine and poly(acrylic acid). Photostimulation of NG108-15, cells cultured on the QD film was achieved by illumination with a 532 nm laser at 800 mW/cm<sup>2</sup> intensity for 500 ms, and recorded using the patch-clamp technique. No oxidative damage or additional cell stimulation was observed upon a train of repetitive pulses. The proposed mechanism for photocurrent generation includes a sequence of photochemical and charge-transfer reactions, and is illustrated in <xref ref-type="fig" rid="f3-ijn-9-065">Figure 3B</xref>. First, electrons and holes are generated due to excitons formation in response to light absorbance in the HgTe QDs. The photogenerated electrons are then transferred to oxygen in solution. This results in accumulation of holes due to slow electron transport through the LBL NP film. Thus, the measured photocurrent originates from electrons injected from the electrode surface in response to hole accumulation.<xref rid="b149-ijn-9-065" ref-type="bibr">149</xref> The authors further suggested resistive coupling as a mechanism of neuronal light stimulation.</p><p>The illumination intensity used for photostimulation was very high, more than an order of magnitude higher than bright daylight intensity (800 mW/cm<sup>2</sup> compared with ~30 mW/cm<sup>2</sup>, respectively). Clearly, the efficiency of the charge transfer between QDs and activated cells needs further optimization. The resistive coupling mechanism includes the transfer of electrons across the electrode–electrolyte interface, and thus requires that species, on the surface of the electrode or in the solution, are oxidized or reduced. These reactions can lead to irreversible processes that cause electrode or tissue damage.</p><p>The LBL approach applied in Pappas et al<xref rid="b149-ijn-9-065" ref-type="bibr">149</xref> was also adopted by Invitrogen (Carlsbad, CA, USA).<xref rid="b155-ijn-9-065" ref-type="bibr">155</xref> A nanostructured biocompatible interface, composed of stacks of semiconductor QDs, coated with an adhesion protein layer was prepared (similar to the scheme illustrated in <xref ref-type="fig" rid="f2-ijn-9-065">Figure 2A</xref> with a protein adhesion layer instead of a polymer-based electrostatic conjugation). NG108 and primary hippocampal neurons were photostimulated.</p><p>Three schemes for optical neuronal activation by QD coated surfaces were described by Lugo et al.<xref rid="b150-ijn-9-065" ref-type="bibr">150</xref> A CdTe QD coated glass or a cadmium selenide (CdSe) QD coated ITO were used as photoactive substrates, on which prostate cancer cells (LnCap) or mice cortical cells were cultured, respectively. The third scheme is based on a glass micropipette probe coated with CdSe QDs. The coated micropipette was placed on a mice cortical cell. Changes in membrane potential and ionic currents, due to light stimulation for all of the described schemes, were recorded by the patch-clamp technique. The CdTe QDs film was fabricated using electrostatic LBL by layering QD films with opposite-polarity surface charges on a glass substrate as illustrated in <xref ref-type="fig" rid="f3-ijn-9-065">Figure 3C</xref>. CdTe QDs were coated with either 2-mercaptoethylamine for positive charges or thioglycolic acid for negative charges. An additional polylysine layer was applied to enhance LnCap cells attachment. A 430 nm illumination resulted in hyperpolarization of the prostate cancer cells which was attributed to activation of potassium channels, present at a high density in prostate cancer cells.<xref rid="b156-ijn-9-065" ref-type="bibr">156</xref> QD stimulation depended on the proximity of the cells to the QD film and disappeared when cells were lifted ~20 μm above the film. Furthermore, using a 740 nm light source (longer than the QD absorption cutoff wavelength) no cellular response was observed, confirming the role of QDs in stimulating the cell.<xref rid="b150-ijn-9-065" ref-type="bibr">150</xref></p><p>A CdSe QD coated ITO substrate was prepared by drop casting, as illustrated in <xref ref-type="fig" rid="f3-ijn-9-065">Figure 3D</xref>. A 550 nm illumination of the film resulted in some mice cortical cells depolarizing and action potential firing, while others hyperpolarized. This variability was attributed to compromised cell viability on the QD film or due to distance variation (between the cells and the substrate).</p><p>Finally, hyperpolarization of mice cortical cell membrane was generated by 550 nm illumination of a CdSe QD coated glass micropipette probe by drop casting (similar to the scheme illustrated in <xref ref-type="fig" rid="f3-ijn-9-065">Figure 3D</xref>, with a glass micropipette instead of an ITO substrate). Photoexcitation of the QD probes often produced little or no response. These results were attributed to variations in the ion channels distribution and cell viability. Excitation effects in all schemes required an intensity of 107 photons/μm<sup>2</sup>/second.<xref rid="b150-ijn-9-065" ref-type="bibr">150</xref></p><p>Studies aiming to achieve neuronal photostimulation using QDs have so far achieved limited efficiency. A key challenge in harnessing QD technology to activate neurons is the efficiency and the nature of the charge transfer mechanisms. Further understanding of the photocurrent and photo stimulation mechanisms is of utmost importance. QD toxicity in neural applications is crucial to establish a biocompatible QD-based photoactive neural interface. Toxic effects of QDs include changes in cell morphology, suppressed metabolic activity, and a decrease in cell viability<xref rid="b157-ijn-9-065" ref-type="bibr">157</xref>,<xref rid="b158-ijn-9-065" ref-type="bibr">158</xref> and are attributed mainly to their composition as well as to their surface coating and nanometer size.<xref rid="b159-ijn-9-065" ref-type="bibr">159</xref></p><p>The most predominant cause for QD cytotoxicity is the generation of ROS. ROS diminish cellular respiration, increase permeation of the cell membrane, and damage the nucleus, leading to eventual cell death.<xref rid="b159-ijn-9-065" ref-type="bibr">159</xref>,<xref rid="b160-ijn-9-065" ref-type="bibr">160</xref> A notable example is the photo-oxidation of cadmium (Cd), a primary constituent in commonly employed QDs (CdSe, CdS, CdTe).<xref rid="b161-ijn-9-065" ref-type="bibr">161</xref> These ions are thought to bind mitochondrial proteins, diminish cellular respiration, and ultimately lead to cell death.<xref rid="b161-ijn-9-065" ref-type="bibr">161</xref></p><p>The ligand coating the QD surface can also have toxic effects. Ligands such as trioctylphosphene oxide and mercaptoundecanoic acid may cause decreased cell proliferation or DNA damage.<xref rid="b162-ijn-9-065" ref-type="bibr">162</xref>,<xref rid="b163-ijn-9-065" ref-type="bibr">163</xref> Particle size also contributes to toxicity, with smaller particles found to be more toxic than larger particles,<xref rid="b157-ijn-9-065" ref-type="bibr">157</xref>,<xref rid="b164-ijn-9-065" ref-type="bibr">164</xref> due to increase in surface to volume ratio,<xref rid="b163-ijn-9-065" ref-type="bibr">163</xref> (more surface Cd<sup>+2</sup> ions are available for reaction). Additional significant challenges include biocompatibility of the whole device and neuron–device coupling.</p></sec></sec></sec><sec><title>Summary, challenges, and perspectives</title><p>In this review we explored different schemes for light activation of neurons, focusing on novel devices and interfaces. We have surveyed the different schemes for neuronal stimulation with light studied so far, and the advantages and challenges of each scheme were discussed. <xref ref-type="table" rid="t1-ijn-9-065">Table 1</xref> summarizes the pros and cons of the different schemes discussed above.</p><p>Recent progress in material engineering and the emergence of new nanomaterials offer many advantages in both light harvesting and interfacing with neurons. <xref ref-type="table" rid="t2-ijn-9-065">Table 2</xref> summarizes the three major schemes for photoactive neuronal surfaces discussed above, including: photoconductive effect of silicon substrate under applied voltage, CPs, and QDs as chromophores for efficient light harvesting and transduction to electrical signal. QDs were either directly conjugated to a neural cell membrane or used to form a photoactive interface.</p><p>Light directed neural interfaces are in the early stages of development, mostly limited to acute or experimental applications. Key challenges must be overcome before these devices can be used in neural implants for vision restoration. In the next sections we will discuss these challenges and further point out some promising directions in the study of photoactive neuronal interfaces. The first major challenge is to achieve efficient light harvesting and transduction of light into an electrical signal. Neural photoelectrodes should also accommodate the dramatic differences between the tissue and the device: the soft nature of living tissues compared with rigid electronic components, as well as ionic charge transport in tissue compared with electrons and holes conduction in solid state electronic devices. Thus, additional challenges include the safety of the electrochemical interface, as well as meeting mechanical, biocompatibility, and stability requirements.</p><p>Light harvesting systems which aim to replace the highly sensitive biological system<xref rid="b165-ijn-9-065" ref-type="bibr">165</xref> face an incredible challenge for the generation of retinal prostheses. Recently proposed approaches are not sensitive enough and the use of new materials such as QDs and CPs offer exciting alternatives. However, understanding the underlying mechanisms of light harvesting, charge separation, and photocurrent generation in these novel nanostructured devices is still incomplete, and needs further research. Indeed, improving charge transfer efficiency of photoexcited QDs has been under investigation in recent years in the field of solar cell technology.<xref rid="b145-ijn-9-065" ref-type="bibr">145</xref>,<xref rid="b166-ijn-9-065" ref-type="bibr">166</xref>,<xref rid="b167-ijn-9-065" ref-type="bibr">167</xref> Such solar cells consist of QD coated electrodes embedded in electrolyte-containing charge donors. In the presence of light, charges are injected from a QD matrix into the underlying support electrode, and direct current between a counter electrode in the solution and the QD coated electrode is obtained.<xref rid="b164-ijn-9-065" ref-type="bibr">164</xref> The QD-electrode-solution coupling is extremely important both for solar cells and neuronal stimulation applications, as the environment may affect charge transport processes as well as the effective band structure of the QDs,<xref rid="b168-ijn-9-065" ref-type="bibr">168</xref>–<xref rid="b170-ijn-9-065" ref-type="bibr">170</xref> and may result in enhanced or suppressed photocurrent.</p><p>As mentioned above, another key barrier that must be overcome for the safe realization of light directed neural interfaces is proper electrochemical charge transfer across the electrode–tissue interface. The electrochemical properties of the stimulating interface, which determine the reactions mediating the transition from electron flow in the electrode to ion flow in the tissue, are fundamental to its performance. The physical reactions taking place during neuronal stimulation, following photoexcitation and photocurrent generation, can be capacitive or faradaic or a combination of the two.<xref rid="b8-ijn-9-065" ref-type="bibr">8</xref>,<xref rid="b171-ijn-9-065" ref-type="bibr">171</xref> The capacitive mechanism involves the charging and discharging of the photoelectrode–electrolyte double layer, and neuronal membrane/electrolyte interfaces due to redistribution of charged species in the electrolyte. The faradaic mechanism, on the other hand, involves the transfer of electrons across the electrode–electrolyte interface and is associated with oxidation or reduction reactions of species on the surface of the photoelectrode or in the solution.<xref rid="b8-ijn-9-065" ref-type="bibr">8</xref>,<xref rid="b171-ijn-9-065" ref-type="bibr">171</xref> These reactions may be irreversible and may result in both electrode damage and cell degradation and should be avoided. Therefore, the mechanism of charge injection, as well as the actual amount of charge, is important. As a rule, the ideal situation for safe and long-term neuronal stimulation is capacitive charge delivery.<xref rid="b7-ijn-9-065" ref-type="bibr">7</xref>,<xref rid="b172-ijn-9-065" ref-type="bibr">172</xref>,<xref rid="b173-ijn-9-065" ref-type="bibr">173</xref></p><p>Other critical parameters are flexibility and mechanical compatibility with the neuronal tissue, which are both crucial stability of the device in a biological environment is important to allow long-term in vitro investigations and clearly for retinal prosthetic application.<xref rid="b10-ijn-9-065" ref-type="bibr">10</xref>,<xref rid="b39-ijn-9-065" ref-type="bibr">39</xref>,<xref rid="b41-ijn-9-065" ref-type="bibr">41</xref></p><p>Primarily, understanding the chronic effects of light is highly important. Exposure to high illumination intensity may result in oxidative changes to the membrane or thermal and photonic damage to cells.<xref rid="b106-ijn-9-065" ref-type="bibr">106</xref> Improvement in the biocompatibility of the photoactive schemes reviewed above remains a major challenge. The photoconductive silicon scheme includes the application of a voltage (see <xref ref-type="fig" rid="f1-ijn-9-065">Figure 1</xref>) which may result in heating of the tissue. CPs may lose stability in an aqueous solution under illumination, resulting in degradation of conduction properties.<xref rid="b131-ijn-9-065" ref-type="bibr">131</xref>,<xref rid="b132-ijn-9-065" ref-type="bibr">132</xref> Furthermore, toxic polymerization residues may not be suitable for long-term cell interfacing applications. Finally QDs, especially free in solution, may induce adverse cytotoxicity effects originating from their toxic composition (core and ligands) or from their nanoscale size.<xref rid="b177-ijn-9-065" ref-type="bibr">177</xref>,<xref rid="b178-ijn-9-065" ref-type="bibr">178</xref> Furthermore, photoexcitation of Cd-based QDs can lead to the generation of free Cd radicals which are thought to bind mitochondrial proteins and may interfere with cellular respiration and lead to cell death.<xref rid="b161-ijn-9-065" ref-type="bibr">161</xref> To reduce QD toxic effects it is possible to use biocompatible surface coatings such as glutathione or polyethyleneimine or to limit access to the toxic core by coating the particles with a protective layer. For example, coating films of CdTe with collagen or ZnS assemblies with bovine serum albumin<xref rid="b157-ijn-9-065" ref-type="bibr">157</xref>,<xref rid="b162-ijn-9-065" ref-type="bibr">162</xref> can improve cell viability. In addition, attachment of QDs to a surface may prevent cellular uptake.</p><p>To summarize, it is critical to ensure efficient light transduction, efficient electrochemical interfacing, and a proper electrode–neuron interface to ensure both the viability of the tissue and the effectiveness of the photoelectrical interface. Beyond the many challenges and technical difficulties discussed above, a critical impediment in realizing visual prostheses is the ability to faithfully mimic the coding of the retina in a manner suitable for image reconstruction by the brain. These challenges should be addressed before this technique can be widely applied in clinical therapy. Despite many challenges, optical techniques present a promising direction to overcome the fundamental limitations of traditional electrophysiological tools by allowing spatial selectivity and fine control over the stimulated area. QD films and CPs offer exciting possibilities in engineering efficient optoelectrical technologies to investigate neuronal activity and even for substitution of degenerated retina. Promising directions for future nanostructured photoactive neuronal interfaces include utilization of photo-responsive nanowires,<xref rid="b180-ijn-9-065" ref-type="bibr">180</xref> carbon nanotubes (CNTs),<xref rid="b181-ijn-9-065" ref-type="bibr">181</xref> and flexible support materials.<xref rid="b182-ijn-9-065" ref-type="bibr">182</xref></p><p>Incorporation of CNTs in the design of a photoactive interface is an approach to enhance the photocurrent and increase biocompatibility. Intensive investigations over the past decade have explored the unique properties of CNTs for neuronal interfacing, including enhanced electrochemical properties, biocompatibility and durability, as well as high electrical conductivity. We have recently reviewed progress in CNT-based microelectrode arrays for neuronal recording and stimulation, and highlighted developments towards a flexible biocompatible CNT-based technology.<xref rid="b183-ijn-9-065" ref-type="bibr">183</xref> CNTs coupled to chromophores, such as QDs, may further enhance the electrical coupling between the chromophore and neurons. This approach is currently being studied by our group for retinal stimulation applications.<xref rid="b181-ijn-9-065" ref-type="bibr">181</xref></p><p>The coupling of QDs to CNTs has been demonstrated.<xref rid="b184-ijn-9-065" ref-type="bibr">184</xref>–<xref rid="b186-ijn-9-065" ref-type="bibr">186</xref> Furthermore, CNTs have been used as a linker between a QD matrix and an electrode enhanced generation of a photocurrent.<xref rid="b187-ijn-9-065" ref-type="bibr">187</xref>,<xref rid="b188-ijn-9-065" ref-type="bibr">188</xref> CNTs provide a very effective adhesive material for neuronal proliferation, and as such, facilitate both strong coupling between the cells and the surface. The inherently electrochemically stable interface, with a capacitive charge transfer mechanism and low impedence is ideal for efficient neuronal stimulation. Improved mechanical and electrical coupling between the cells and the electrodes is also expected when using the CNT–QD scheme. For long-term neural in vivo applications, the flexibility of the photoactive device is highly desirable. Recently, our group has developed a flexible electrode array suited for neuronal recording and stimulation and composed entirely of CNTs.<xref rid="b182-ijn-9-065" ref-type="bibr">182</xref> Our technology is simple, robust and the resulting stimulating electrodes are nearly purely capacitive. The device was successfully applied for recording and stimulation of a chick retina.<xref rid="b182-ijn-9-065" ref-type="bibr">182</xref></p><p>To conclude, photoactive surfaces have an enormous potential in the realm of neuronal interfaces. The emergence of novel nanomaterials, combined with the implementation of flexible and transparent device support, offers exciting new solutions addressing issues such as efficient light harvesting and transduction to electrical signals, long-term biocompatibility and stability, small size, and improved mechanical properties. The future of these bio-interfacing optoelectronic devices is based upon expanding our understanding of the mechanisms underlying the generation of photocurrent and neuronal photostimulation. At this early stage of the technology, it is still difficult to evaluate which of the many suggested technologies would be the optimal one. As we have discussed above, each technology has advantages and disadvantages, but the overall compatibility of these technologies will have to be revealed through additional testing and long-term use. Additional research would eventually result in more robust technology and may revolutionize the treatment of retinal impairments.</p></sec></body><back><ack><title>Acknowledgments</title><p>The authors are grateful for many useful discussions with Evelyne Sernagor, Uri Banin, Ori Cheshnovsky, and Shlomo Yitzchaik. 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(<bold>B</bold>) A different scheme demonstrated by Starovoytov et al (2005),<xref rid="b94-ijn-9-065" ref-type="bibr">94</xref> using a fixed voltage between a p-type silicon and a Ag/AgCl reference electrode and modulating a laser beam illumination. (<bold>C</bold>) An illustration of neuronal cells recording using light addressable TiN electrodes on an a-Si:H photoconductive layer presented by Bucher et al (2001),<xref rid="b95-ijn-9-065" ref-type="bibr">95</xref>,<xref rid="b96-ijn-9-065" ref-type="bibr">96</xref> (<bold>D</bold>) An illustration of neuronal cells light activation using a-Si:H photoconductivity according to the scheme based on Suzurikawa et al (2007).<xref rid="b98-ijn-9-065" ref-type="bibr">98</xref> In both (<bold>C</bold> and <bold>D</bold>), passivation is required due to the sensitivity of the a-Si:H layer to aqueous environment. In all schemes, conductivity is increased in the illuminated area, (under application of a voltage bias) resulting in neuronal stimulation or recording.</p><p><bold>Abbreviations:</bold> a-Si:H, hydrogenated amorphous silicon; ITO, indium tin oxide; TiN, titanium nitride; SnO<sub>2</sub>, tin oxide; Si, silicon; Vb, voltage bias.</p></caption><graphic xlink:href="ijn-9-065Fig1"></graphic></fig><fig id="f2-ijn-9-065" position="float"><label>Figure 2</label><caption><p>Conducting polymer for neuronal photoactivation.</p><p><bold>Notes:</bold> An illustration of neuronal light activation using the scheme demonstrated by Ghezzi et al.<xref rid="b127-ijn-9-065" ref-type="bibr">127</xref> Illumination of the polymer results in charge separation leading to neuronal stimulation.</p><p><bold>Abbreviations:</bold> ITO, indium tin oxide; P3HT:PCBM, poly(3- hexylthiophene): phenyl-C61-butyric-acid-methyl ester.</p></caption><graphic xlink:href="ijn-9-065Fig2"></graphic></fig><fig id="f3-ijn-9-065" position="float"><label>Figure 3</label><caption><p>Quantum dots for neuronal photoactivation.</p><p><bold>Notes:</bold> (<bold>A</bold>) Direct wiring of QDs to neuronal cell membrane according to the scheme studied by Winter et al (2001).<xref rid="b147-ijn-9-065" ref-type="bibr">147</xref> Illumination results in charge separation in the QDs, electric field buildup and cell activation. (<bold>B</bold>) QD coated device according to the scheme reported by Pappas et al (2007).<xref rid="b149-ijn-9-065" ref-type="bibr">149</xref> A QD film is composed of layers of QDs and a polymer. Upon illumination the photogenerated electrons are transferred to oxygen in the solution. This results in accumulation of holes. The photocurrent, activating the neuronal cells originates from the electrons injected from the ITO surface in response to the accumulation of holes. (<bold>C</bold>) A QD film composed of layers of QDs with opposite-polarity surface coating according to the scheme presented by Lugo et al (2012).<xref rid="b150-ijn-9-065" ref-type="bibr">150</xref> (<bold>D</bold>) An alternative route to QD film deposition, also studied by Lugo et al (2012),<xref rid="b150-ijn-9-065" ref-type="bibr">150</xref> prepared by drop coating. This approach was also used for coating of a glass micropipette probe. In both (<bold>C</bold> and <bold>D</bold>), light generated dipoles in the QD layer are associated with an electric fields designed to activate neurons when placed close enough to the cells.</p><p><bold>Abbreviations:</bold> ITO, indium tin oxide; QD, quantum dot.</p></caption><graphic xlink:href="ijn-9-065Fig3"></graphic></fig><table-wrap id="t1-ijn-9-065" position="float"><label>Table 1</label><caption><p>Summary of neuronal optical stimulation schemes</p></caption><table frame="hsides" rules="groups"><thead><tr><th align="left" valign="top" rowspan="1" colspan="1">Optical stimulation scheme</th><th align="left" valign="top" rowspan="1" colspan="1">Pros</th><th align="left" valign="top" rowspan="1" colspan="1">Cons</th></tr></thead><tbody><tr><td colspan="3" align="left" valign="top" rowspan="1">Retinal implants</td></tr><tr><td align="left" valign="top" rowspan="1" colspan="1"> External light capturing device</td><td align="left" valign="top" rowspan="1" colspan="1">– Mature technology<break></break>– High charge injection<break></break>– Variable stimulation patterns</td><td align="left" valign="top" rowspan="1" colspan="1">– Low spatial resolution<break></break>– Wiring<break></break>– Invasive<break></break>– Rigid</td></tr><tr><td align="left" valign="top" rowspan="1" colspan="1"> Photodiodes</td><td align="left" valign="top" rowspan="1" colspan="1">– Mature technology<break></break>– Compact<break></break>– Large electrode array<break></break>– Variable stimulation patterns</td><td align="left" valign="top" rowspan="1" colspan="1">– Wiring<break></break>– Invasive<break></break>– Rigid</td></tr><tr><td colspan="3" align="left" valign="top" rowspan="1">Light directed neuronal stimulation</td></tr><tr><td align="left" valign="top" rowspan="1" colspan="1"> Optogenetics</td><td align="left" valign="top" rowspan="1" colspan="1">– High temporal and spatial resolution<break></break>– Cell specificity<break></break>– Minimally invasive</td><td align="left" valign="top" rowspan="1" colspan="1">– Long-term expression of light sensitive proteins<break></break>– Immune system response</td></tr><tr><td align="left" valign="top" rowspan="1" colspan="1"> Caged glutamate</td><td align="left" valign="top" rowspan="1" colspan="1">– High spatial resolution<break></break>– Minimally invasive</td><td align="left" valign="top" rowspan="1" colspan="1">– Low temporal resolution<break></break>– Poor cell selectivity<break></break>– Need for repeated injection of neurotransmitters</td></tr><tr><td align="left" valign="top" rowspan="1" colspan="1"> Infrared light</td><td align="left" valign="top" rowspan="1" colspan="1">– High spatial resolution<break></break>– Noninvasive</td><td align="left" valign="top" rowspan="1" colspan="1">– Light source should be at close proximity<break></break>– Thermal tissue damage<break></break>– Technical difficulty in realizing high density prosthesis</td></tr><tr><td colspan="3" align="left" valign="top" rowspan="1">Photoactive surfaces</td></tr><tr><td align="left" valign="top" rowspan="1" colspan="1"> Photoconductive silicon</td><td align="left" valign="top" rowspan="1" colspan="1">– High spatial resolution<break></break>– Simple</td><td align="left" valign="top" rowspan="1" colspan="1">– Limited temporal resolution<break></break>– Limited stability<break></break>– Wiring<break></break>– Invasive<break></break>– Rigid</td></tr><tr><td align="left" valign="top" rowspan="1" colspan="1"> Conducting polymers</td><td align="left" valign="top" rowspan="1" colspan="1">– High spatial resolution<break></break>– Simple fabrication and deposition<break></break>– Flexible</td><td align="left" valign="top" rowspan="1" colspan="1">– Low stability upon continuous stimulation<break></break>– Toxic polymerization residues<break></break>– Invasive</td></tr><tr><td align="left" valign="top" rowspan="1" colspan="1"> Quantum dots</td><td align="left" valign="top" rowspan="1" colspan="1">– High efficiency<break></break>– Stable<break></break>– Flexible</td><td align="left" valign="top" rowspan="1" colspan="1">– Toxic<break></break>– Invasive</td></tr></tbody></table></table-wrap><table-wrap id="t2-ijn-9-065" position="float"><label>Table 2</label><caption><p>Photoactive neuronal surfaces</p></caption><table frame="hsides" rules="groups"><thead><tr><th align="left" valign="top" rowspan="1" colspan="1">Reference</th><th align="left" valign="top" rowspan="1" colspan="1">Light stimulation scheme</th><th align="left" valign="top" rowspan="1" colspan="1">Photocurrent mechanism</th><th align="left" valign="top" rowspan="1" colspan="1">In vitro biotesting</th><th align="left" valign="top" rowspan="1" colspan="1">Recording</th><th align="left" valign="top" rowspan="1" colspan="1">Applied bias [V]</th><th align="left" valign="top" rowspan="1" colspan="1">Neuronal light stimulation</th></tr></thead><tbody><tr><td colspan="7" align="left" valign="top" rowspan="1">Photoconductive silicon</td></tr><tr><td align="left" valign="top" rowspan="1" colspan="1"> Colicos et al<xref rid="b92-ijn-9-065" ref-type="bibr">92</xref></td><td align="left" valign="top" rowspan="1" colspan="1">p-type Si using constant illumination and modulation of voltage bias</td><td align="left" valign="top" rowspan="1" colspan="1">Photoconductivity</td><td align="left" valign="top" rowspan="1" colspan="1">Rat hippocampal cultures</td><td align="left" valign="top" rowspan="1" colspan="1">Calcium imaging</td><td align="left" valign="top" rowspan="1" colspan="1">+4</td><td align="left" valign="top" rowspan="1" colspan="1">Achieved</td></tr><tr><td align="left" valign="top" rowspan="1" colspan="1"> Starovoytov et al<xref rid="b94-ijn-9-065" ref-type="bibr">94</xref></td><td align="left" valign="top" rowspan="1" colspan="1">p-type Si under constant voltage bias and modulation of illumination</td><td align="left" valign="top" rowspan="1" colspan="1">Photoconductivity</td><td align="left" valign="top" rowspan="1" colspan="1">Rat hippocampal cultures</td><td align="left" valign="top" rowspan="1" colspan="1">Patch-clamp</td><td align="left" valign="top" rowspan="1" colspan="1">−0.4 to −1.5</td><td align="left" valign="top" rowspan="1" colspan="1">Achieved</td></tr><tr><td align="left" valign="top" rowspan="1" colspan="1"> Bucher et al<xref rid="b96-ijn-9-065" ref-type="bibr">96</xref>,<xref rid="b104-ijn-9-065" ref-type="bibr">104</xref></td><td align="left" valign="top" rowspan="1" colspan="1">a-Si:H layer between TiN microelectrode array and ITO leads on glass; SiOx:C insulation</td><td align="left" valign="top" rowspan="1" colspan="1">Photoconductivity for light addressed recording of neuronal spontaneous activity</td><td align="left" valign="top" rowspan="1" colspan="1">Cardiac myocytes</td><td align="left" valign="top" rowspan="1" colspan="1">Extracellular using the light sensitive electrode array</td><td align="left" valign="top" rowspan="1" colspan="1">−0.2 to +0.2</td><td align="left" valign="top" rowspan="1" colspan="1">Not achieved</td></tr><tr><td align="left" valign="top" rowspan="1" colspan="1"> Suzurikawa et al<xref rid="b97-ijn-9-065" ref-type="bibr">97</xref>,<xref rid="b98-ijn-9-065" ref-type="bibr">98</xref></td><td align="left" valign="top" rowspan="1" colspan="1">a-Si:H layer on SnO<sub>2</sub> substrate; spin-coated ZnOSb<sub>2</sub>O<sub>5</sub> insulation</td><td align="left" valign="top" rowspan="1" colspan="1">Photoconductivity</td><td align="left" valign="top" rowspan="1" colspan="1">Embryonic rat cortical cultures</td><td align="left" valign="top" rowspan="1" colspan="1">Calcium imaging</td><td align="left" valign="top" rowspan="1" colspan="1">+3</td><td align="left" valign="top" rowspan="1" colspan="1">Achieved</td></tr><tr><td colspan="7" align="left" valign="top" rowspan="1">Conducting polymer</td></tr><tr><td align="left" valign="top" rowspan="1" colspan="1"> Ghezzi et al<xref rid="b127-ijn-9-065" ref-type="bibr">127</xref></td><td align="left" valign="top" rowspan="1" colspan="1">P3HT:PCBM on ITO coated glass</td><td align="left" valign="top" rowspan="1" colspan="1">Donor-acceptor mechanism in bulk heterojunction polymer</td><td align="left" valign="top" rowspan="1" colspan="1">Rat hippocampal cultures</td><td align="left" valign="top" rowspan="1" colspan="1">Patch-clamp</td><td align="left" valign="top" rowspan="1" colspan="1">Not biased</td><td align="left" valign="top" rowspan="1" colspan="1">Achieved</td></tr><tr><td align="left" valign="top" rowspan="2" colspan="1"> Ghezzi et al<xref rid="b128-ijn-9-065" ref-type="bibr">128</xref></td><td align="left" valign="top" rowspan="2" colspan="1">P3HT on ITO coated glass</td><td align="left" valign="top" rowspan="2" colspan="1">Charge separation occurring mainly at the P3HT-ITO interface</td><td align="left" valign="top" rowspan="1" colspan="1">Rat hippocampal cultures</td><td align="left" valign="top" rowspan="1" colspan="1">Patch-clamp</td><td align="left" valign="top" rowspan="1" colspan="1">Not biased</td><td align="left" valign="top" rowspan="1" colspan="1">Achieved</td></tr><tr><td align="left" valign="top" rowspan="1" colspan="1">Mice degenerated retina</td><td align="left" valign="top" rowspan="1" colspan="1">Patch-clamp</td><td align="left" valign="top" rowspan="1" colspan="1">Not biased</td><td align="left" valign="top" rowspan="1" colspan="1">Achieved</td></tr><tr><td colspan="7" align="left" valign="top" rowspan="1">Quantum dots</td></tr><tr><td align="left" valign="top" rowspan="1" colspan="1"> Winter et al<xref rid="b147-ijn-9-065" ref-type="bibr">147</xref></td><td align="left" valign="top" rowspan="1" colspan="1">CdS QDs directly wired to neurons by antibody or peptide recognition</td><td align="left" valign="top" rowspan="1" colspan="1">Electrical field generated by photoexcited charge separation in QDs may activate cells</td><td align="left" valign="top" rowspan="1" colspan="1">SK-N-SH human neuroblastoma cells</td><td align="left" valign="top" rowspan="1" colspan="1">–</td><td align="left" valign="top" rowspan="1" colspan="1">Not biased</td><td align="left" valign="top" rowspan="1" colspan="1">Not achieved</td></tr><tr><td align="left" valign="top" rowspan="1" colspan="1"> Pappas et al<xref rid="b149-ijn-9-065" ref-type="bibr">149</xref></td><td align="left" valign="top" rowspan="1" colspan="1">HgTe QDs-PDDA LBL film on ITO substrate</td><td align="left" valign="top" rowspan="1" colspan="1">Photogenerated electrons in HgTe QDs transferred to O<sub>2</sub> in solution result with holes accumulation. Electrons are injected from the electrode surface in response to this holes accumulation.</td><td align="left" valign="top" rowspan="1" colspan="1">NG108-15 human neuroblastima</td><td align="left" valign="top" rowspan="1" colspan="1">Patch-clamp</td><td align="left" valign="top" rowspan="1" colspan="1">Not biased</td><td align="left" valign="top" rowspan="1" colspan="1">Achieved</td></tr><tr><td align="left" valign="top" rowspan="1" colspan="1"> Molokanova et al<xref rid="b155-ijn-9-065" ref-type="bibr">155</xref></td><td align="left" valign="top" rowspan="1" colspan="1">QD LBL film</td><td align="left" valign="top" rowspan="1" colspan="1">Electrical fields generated by photoexcited QDs</td><td align="left" valign="top" rowspan="1" colspan="1">Primary hippocampal cultures</td><td align="left" valign="top" rowspan="1" colspan="1">Calcium imaging and intracellular recording</td><td align="left" valign="top" rowspan="1" colspan="1">Not biased</td><td align="left" valign="top" rowspan="1" colspan="1">Achieved</td></tr><tr><td align="left" valign="top" rowspan="3" colspan="1"> Lugo et al<xref rid="b150-ijn-9-065" ref-type="bibr">150</xref></td><td align="left" valign="top" rowspan="3" colspan="1">CdTe QDs with opposite charge coating LBL film on glass substrate CdSe coated ITO by drop casting CdSe coated glass micropipette drop casting, placed on neural cells</td><td align="left" valign="top" rowspan="3" colspan="1">Electrical fields associated with photogenerated dipoles on QD film</td><td align="left" valign="top" rowspan="1" colspan="1">Prostate cancer cells (LnCap)</td><td align="left" valign="top" rowspan="1" colspan="1">Patch-clamp</td><td align="left" valign="top" rowspan="1" colspan="1">Not biased</td><td align="left" valign="top" rowspan="1" colspan="1">Achieved</td></tr><tr><td align="left" valign="top" rowspan="1" colspan="1">Mice cortical culture</td><td align="left" valign="top" rowspan="1" colspan="1">Patch-clamp</td><td align="left" valign="top" rowspan="1" colspan="1"></td><td align="left" valign="top" rowspan="1" colspan="1">Achieved</td></tr><tr><td align="left" valign="top" rowspan="1" colspan="1">Mice cortical culture</td><td align="left" valign="top" rowspan="1" colspan="1">Patch-clamp</td><td align="left" valign="top" rowspan="1" colspan="1"></td><td align="left" valign="top" rowspan="1" colspan="1">Achieved</td></tr></tbody></table><table-wrap-foot><fn id="tfn1-ijn-9-065"><p><bold>Abbreviations:</bold> a-Si:H, hydrogenated amorphous silicon; CdS, cadmium sulfide; HgTe, mercury telluride; CdTe, cadmium telluride, CdSe, cadmium selenide; ITO, indium tin oxide; LBL, layer-by-layer; QD, quantum dot; P3HT:PCBM, poly(3- hexylthiophene): phenyl-C61-butyric-acid-methyl ester; PDDA, poly(dimethyl-diallyl-ammonium-chloride); SiOx:C, carbonic silicon oxide; TiN, titanium nitride.</p></fn></table-wrap-foot></table-wrap></floats-group></pmc></record>Pour manipuler ce document sous Unix (Dilib)
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