Multicolor Upconversion Nanoparticles for Protein Conjugation
Identifieur interne : 000396 ( Pmc/Corpus ); précédent : 000395; suivant : 000397Multicolor Upconversion Nanoparticles for Protein Conjugation
Auteurs : Stefan Wilhelm ; Thomas Hirsch ; Wendy M. Patterson ; Elisabeth Scheucher ; Torsten Mayr ; Otto S. WolfbeisSource :
- Theranostics [ 1838-7640 ] ; 2013.
Abstract
We describe the preparation of monodisperse, lanthanide-doped hexagonal-phase NaYF4 upconverting luminescent nanoparticles for protein conjugation. Their core was coated with a silica shell which then was modified with a poly(ethylene glycol) spacer and N-hydroxysuccinimide ester groups. The nanoparticles were characterized by transmission electron microscopy, Raman spectroscopy, X-ray diffraction, and dynamic light scattering. The N-hydroxysuccinimide ester functionalization renders them highly reactive towards amine nucleophiles (e.g., proteins). We show that such particles can be conjugated to proteins. The protein-reactive UCLNPs and their conjugates to streptavidin and bovine serum albumin display multicolor emissions upon 980-nm continuous wave laser excitation. Surface plasmon resonance studies were carried out to prove bioconjugation and to compare the affinity of the particles for proteins immobilized on a thin gold film.
Url:
DOI: 10.7150/thno.5113
PubMed: 23606910
PubMed Central: 3630524
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PMC:3630524Le document en format XML
<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">Multicolor Upconversion Nanoparticles for Protein Conjugation</title><author><name sortKey="Wilhelm, Stefan" sort="Wilhelm, Stefan" uniqKey="Wilhelm S" first="Stefan" last="Wilhelm">Stefan Wilhelm</name><affiliation><nlm:aff id="A1">1. University of Regensburg, Institute of Analytical Chemistry, Chemo- and Biosensors, 93040 Regensburg, Germany.</nlm:aff></affiliation></author><author><name sortKey="Hirsch, Thomas" sort="Hirsch, Thomas" uniqKey="Hirsch T" first="Thomas" last="Hirsch">Thomas Hirsch</name><affiliation><nlm:aff id="A1">1. University of Regensburg, Institute of Analytical Chemistry, Chemo- and Biosensors, 93040 Regensburg, Germany.</nlm:aff></affiliation></author><author><name sortKey="Patterson, Wendy M" sort="Patterson, Wendy M" uniqKey="Patterson W" first="Wendy M." last="Patterson">Wendy M. Patterson</name><affiliation><nlm:aff id="A1">1. University of Regensburg, Institute of Analytical Chemistry, Chemo- and Biosensors, 93040 Regensburg, Germany.</nlm:aff></affiliation></author><author><name sortKey="Scheucher, Elisabeth" sort="Scheucher, Elisabeth" uniqKey="Scheucher E" first="Elisabeth" last="Scheucher">Elisabeth Scheucher</name><affiliation><nlm:aff id="A2">2. Graz University of Technology, Institute of Analytical Chemistry and Food Chemistry, 8010 Graz, Austria.</nlm:aff></affiliation></author><author><name sortKey="Mayr, Torsten" sort="Mayr, Torsten" uniqKey="Mayr T" first="Torsten" last="Mayr">Torsten Mayr</name><affiliation><nlm:aff id="A2">2. Graz University of Technology, Institute of Analytical Chemistry and Food Chemistry, 8010 Graz, Austria.</nlm:aff></affiliation></author><author><name sortKey="Wolfbeis, Otto S" sort="Wolfbeis, Otto S" uniqKey="Wolfbeis O" first="Otto S." last="Wolfbeis">Otto S. Wolfbeis</name><affiliation><nlm:aff id="A1">1. University of Regensburg, Institute of Analytical Chemistry, Chemo- and Biosensors, 93040 Regensburg, Germany.</nlm:aff></affiliation></author></titleStmt><publicationStmt><idno type="wicri:source">PMC</idno><idno type="pmid">23606910</idno><idno type="pmc">3630524</idno><idno type="url">http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3630524</idno><idno type="RBID">PMC:3630524</idno><idno type="doi">10.7150/thno.5113</idno><date when="2013">2013</date><idno type="wicri:Area/Pmc/Corpus">000396</idno><idno type="wicri:explorRef" wicri:stream="Pmc" wicri:step="Corpus" wicri:corpus="PMC">000396</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en" level="a" type="main">Multicolor Upconversion Nanoparticles for Protein Conjugation</title><author><name sortKey="Wilhelm, Stefan" sort="Wilhelm, Stefan" uniqKey="Wilhelm S" first="Stefan" last="Wilhelm">Stefan Wilhelm</name><affiliation><nlm:aff id="A1">1. University of Regensburg, Institute of Analytical Chemistry, Chemo- and Biosensors, 93040 Regensburg, Germany.</nlm:aff></affiliation></author><author><name sortKey="Hirsch, Thomas" sort="Hirsch, Thomas" uniqKey="Hirsch T" first="Thomas" last="Hirsch">Thomas Hirsch</name><affiliation><nlm:aff id="A1">1. University of Regensburg, Institute of Analytical Chemistry, Chemo- and Biosensors, 93040 Regensburg, Germany.</nlm:aff></affiliation></author><author><name sortKey="Patterson, Wendy M" sort="Patterson, Wendy M" uniqKey="Patterson W" first="Wendy M." last="Patterson">Wendy M. Patterson</name><affiliation><nlm:aff id="A1">1. University of Regensburg, Institute of Analytical Chemistry, Chemo- and Biosensors, 93040 Regensburg, Germany.</nlm:aff></affiliation></author><author><name sortKey="Scheucher, Elisabeth" sort="Scheucher, Elisabeth" uniqKey="Scheucher E" first="Elisabeth" last="Scheucher">Elisabeth Scheucher</name><affiliation><nlm:aff id="A2">2. Graz University of Technology, Institute of Analytical Chemistry and Food Chemistry, 8010 Graz, Austria.</nlm:aff></affiliation></author><author><name sortKey="Mayr, Torsten" sort="Mayr, Torsten" uniqKey="Mayr T" first="Torsten" last="Mayr">Torsten Mayr</name><affiliation><nlm:aff id="A2">2. Graz University of Technology, Institute of Analytical Chemistry and Food Chemistry, 8010 Graz, Austria.</nlm:aff></affiliation></author><author><name sortKey="Wolfbeis, Otto S" sort="Wolfbeis, Otto S" uniqKey="Wolfbeis O" first="Otto S." last="Wolfbeis">Otto S. Wolfbeis</name><affiliation><nlm:aff id="A1">1. University of Regensburg, Institute of Analytical Chemistry, Chemo- and Biosensors, 93040 Regensburg, Germany.</nlm:aff></affiliation></author></analytic><series><title level="j">Theranostics</title><idno type="eISSN">1838-7640</idno><imprint><date when="2013">2013</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en"><p>We describe the preparation of monodisperse, lanthanide-doped hexagonal-phase NaYF<sub>4</sub> upconverting luminescent nanoparticles for protein conjugation. Their core was coated with a silica shell which then was modified with a poly(ethylene glycol) spacer and N-hydroxysuccinimide ester groups. The nanoparticles were characterized by transmission electron microscopy, Raman spectroscopy, X-ray diffraction, and dynamic light scattering. The N-hydroxysuccinimide ester functionalization renders them highly reactive towards amine nucleophiles (e.g., proteins). We show that such particles can be conjugated to proteins. The protein-reactive UCLNPs and their conjugates to streptavidin and bovine serum albumin display multicolor emissions upon 980-nm continuous wave laser excitation. Surface plasmon resonance studies were carried out to prove bioconjugation and to compare the affinity of the particles for proteins immobilized on a thin gold film.</p></div></front><back><div1 type="bibliography"><listBibl><biblStruct><analytic><author><name sortKey="Lee, J H" uniqKey="Lee J">J-H Lee</name></author><author><name sortKey="Huh, Y M" uniqKey="Huh Y">Y-M Huh</name></author><author><name sortKey="Jun, Y" uniqKey="Jun Y">Y Jun</name></author><author><name sortKey="Seo, J" uniqKey="Seo J">J Seo</name></author><author><name sortKey="Jang, J" uniqKey="Jang J">J Jang</name></author><author><name sortKey="Song, H T" uniqKey="Song H">H-T Song</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Medintz, Il" uniqKey="Medintz I">IL Medintz</name></author><author><name sortKey="Uyeda, Ht" uniqKey="Uyeda H">HT Uyeda</name></author><author><name sortKey="Goldman, Er" uniqKey="Goldman E">ER Goldman</name></author><author><name sortKey="Mattoussi, H" 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<front><journal-meta><journal-id journal-id-type="nlm-ta">Theranostics</journal-id><journal-id journal-id-type="iso-abbrev">Theranostics</journal-id><journal-id journal-id-type="publisher-id">thno</journal-id><journal-title-group><journal-title>Theranostics</journal-title></journal-title-group><issn pub-type="epub">1838-7640</issn><publisher><publisher-name>Ivyspring International Publisher</publisher-name><publisher-loc>Sydney</publisher-loc></publisher></journal-meta><article-meta><article-id pub-id-type="pmid">23606910</article-id><article-id pub-id-type="pmc">3630524</article-id><article-id pub-id-type="doi">10.7150/thno.5113</article-id><article-id pub-id-type="publisher-id">thnov03p0239</article-id><article-categories><subj-group subj-group-type="heading"><subject>Research Paper</subject></subj-group></article-categories><title-group><article-title>Multicolor Upconversion Nanoparticles for Protein Conjugation</article-title></title-group><contrib-group><contrib contrib-type="author"><name><surname>Wilhelm</surname><given-names>Stefan</given-names></name><xref ref-type="aff" rid="A1">1</xref></contrib><contrib contrib-type="author"><name><surname>Hirsch</surname><given-names>Thomas</given-names></name><xref ref-type="aff" rid="A1">1</xref></contrib><contrib contrib-type="author"><name><surname>Patterson</surname><given-names>Wendy M.</given-names></name><xref ref-type="aff" rid="A1">1</xref></contrib><contrib contrib-type="author"><name><surname>Scheucher</surname><given-names>Elisabeth</given-names></name><xref ref-type="aff" rid="A2">2</xref></contrib><contrib contrib-type="author"><name><surname>Mayr</surname><given-names>Torsten</given-names></name><xref ref-type="aff" rid="A2">2</xref></contrib><contrib contrib-type="author"><name><surname>Wolfbeis</surname><given-names>Otto S.</given-names></name><xref ref-type="aff" rid="A1">1</xref><xref ref-type="corresp" rid="FNA_envelop">✉</xref></contrib></contrib-group><aff id="A1">1. University of Regensburg, Institute of Analytical Chemistry, Chemo- and Biosensors, 93040 Regensburg, Germany.</aff><aff id="A2">2. Graz University of Technology, Institute of Analytical Chemistry and Food Chemistry, 8010 Graz, Austria.</aff><author-notes><corresp id="FNA_envelop">✉ Corresponding author: <email>otto.wolfbeis@ur.de</email>; <ext-link ext-link-type="uri" xlink:href="http://www.wolfbeis.de">www.wolfbeis.de</ext-link>.</corresp><fn fn-type="conflict"><p>Competing Interests: The authors have declared that no competing interests do exist.</p></fn></author-notes><pub-date pub-type="collection"><year>2013</year></pub-date><pub-date pub-type="epub"><day>13</day><month>3</month><year>2013</year></pub-date><volume>3</volume><issue>4</issue><fpage>239</fpage><lpage>248</lpage><history><date date-type="received"><day>27</day><month>8</month><year>2012</year></date><date date-type="accepted"><day>23</day><month>9</month><year>2012</year></date></history><permissions><copyright-statement>© Ivyspring International Publisher. This is an open-access article distributed under the terms of the Creative Commons License (http://creativecommons.org/licenses/by-nc-nd/3.0/). Reproduction is permitted for personal, noncommercial use, provided that the article is in whole, unmodified, and properly cited.</copyright-statement><copyright-year>2013</copyright-year></permissions><abstract><p>We describe the preparation of monodisperse, lanthanide-doped hexagonal-phase NaYF<sub>4</sub> upconverting luminescent nanoparticles for protein conjugation. Their core was coated with a silica shell which then was modified with a poly(ethylene glycol) spacer and N-hydroxysuccinimide ester groups. The nanoparticles were characterized by transmission electron microscopy, Raman spectroscopy, X-ray diffraction, and dynamic light scattering. The N-hydroxysuccinimide ester functionalization renders them highly reactive towards amine nucleophiles (e.g., proteins). We show that such particles can be conjugated to proteins. The protein-reactive UCLNPs and their conjugates to streptavidin and bovine serum albumin display multicolor emissions upon 980-nm continuous wave laser excitation. Surface plasmon resonance studies were carried out to prove bioconjugation and to compare the affinity of the particles for proteins immobilized on a thin gold film.</p></abstract><kwd-group><kwd>Upconversion luminescence</kwd><kwd>nanoparticle</kwd><kwd>silica coating</kwd><kwd>Raman spectroscopy</kwd><kwd>biosensing</kwd><kwd>magnetic separation</kwd><kwd>surface plasmon resonance.</kwd></kwd-group></article-meta></front><body><sec><title>Introduction</title><p>The implementation of nanotechnology to healthcare holds great promise in areas such as imaging <xref ref-type="bibr" rid="B1">1</xref>,<xref ref-type="bibr" rid="B2">2</xref>,<xref ref-type="bibr" rid="B3">3</xref>,<xref ref-type="bibr" rid="B4">4</xref>,<xref ref-type="bibr" rid="B5">5</xref>, faster diagnosis <xref ref-type="bibr" rid="B6">6</xref>, targeting <xref ref-type="bibr" rid="B7">7</xref>, drug delivery <xref ref-type="bibr" rid="B8">8</xref>, and tissue regeneration <xref ref-type="bibr" rid="B9">9</xref>, as well as the development of medical products <xref ref-type="bibr" rid="B10">10</xref>,<xref ref-type="bibr" rid="B11">11</xref>,<xref ref-type="bibr" rid="B12">12</xref>. The chemical synthesis of NPs has been studied in detail during the last decade <xref ref-type="bibr" rid="B13">13</xref>,<xref ref-type="bibr" rid="B14">14</xref>,<xref ref-type="bibr" rid="B15">15</xref>,<xref ref-type="bibr" rid="B16">16</xref>. Substantial efforts have been made to control the dimensions, shape, composition, particle size distribution, etc., of NPs, thereby creating new materials with size dependent electrical, optical, magnetic, catalytic, and chemical properties, which cannot be achieved by their bulk counterparts. Important classes of NPs are a) magnetic NPs, b) gold NPs, c) quantum dots, d) silica NPs, etc. <xref ref-type="bibr" rid="B17">17</xref>,<xref ref-type="bibr" rid="B18">18</xref>,<xref ref-type="bibr" rid="B19">19</xref>,<xref ref-type="bibr" rid="B20">20</xref>,<xref ref-type="bibr" rid="B21">21</xref>,<xref ref-type="bibr" rid="B22">22</xref>,<xref ref-type="bibr" rid="B23">23</xref>. In recent years, upconverting luminescent nanoparticles (UCLNPs) joined this classification. Photon upconversion has been researched ever since the 1960s. It is a process where two or more photons are sequentially absorbed, resulting in the emission of light at a shorter wavelength than the excitation light. For instance, infrared or near-infrared (NIR) light can be converted to shorter-wavelength radiation, usually in the visible range of the electromagnetic spectrum (anti-Stokes type emission) <xref ref-type="bibr" rid="B24">24</xref>.</p><p>The mechanisms behind photon upconversion were first investigated in lanthanide-doped bulk materials by Auzel, Ovsyankin, and Feofilov <xref ref-type="bibr" rid="B25">25</xref>. In a sensitizer/activator system, the excitation energy is absorbed by a sensitizer ion (e.g., Yb<sup>3+</sup>) and transferred to an activator ion (e.g., Er<sup>3+</sup> or Tm<sup>3+</sup>) via a non-radiative, resonant energy transfer process. Metastable, long-lived energy states are required, in which case energy transfer upconversion (ETU) is possible, where the combined energies of pump photons are stored, which can lead to the emission of a higher energy photon <xref ref-type="bibr" rid="B26">26</xref>.</p><p>Anti-Stokes emissions from UCLNPs offer several advantages over conventional Stokes-shifted emissions from a) semiconductor quantum dots, b) organic- and protein-based fluorophores, and c) the multiphoton process employing fluorescent dyes. UCLNPs are very attractive phosphors in terms of bioimaging due to their nonblinking emission and remarkable photostability <xref ref-type="bibr" rid="B27">27</xref>,<xref ref-type="bibr" rid="B28">28</xref>,<xref ref-type="bibr" rid="B29">29</xref>. In biological samples or tissue, there is minimal excitation of autofluorophores, since UCLNPs are usually excited by NIR (980 nm) continuous wave (cw) laser light. This scheme enables luminescence to be imaged with a high signal to noise ratio, minimizes possible photodamage in biological systems, and allows deeper tissue penetration <xref ref-type="bibr" rid="B30">30</xref>,<xref ref-type="bibr" rid="B31">31</xref>. Upconversion microparticles have been used before in immunoassays <xref ref-type="bibr" rid="B32">32</xref> and enzyme activity assays <xref ref-type="bibr" rid="B33">33</xref>, but their size (1 - 10 µm) and large size distribution makes their use less attractive. With respect to the relative size of a protein and upconversion <italic>micro</italic>particles, one may not speak of a label in its classical sense.</p><p>High-quality UCLNPs (with respect to crystal phase, monodispersity, geometry, etc.) are usually synthesized in high-boiling organic solvents (e.g., 1-octadecene) using ligand molecules with long alkyl chains (e.g., oleic acid), which renders them inherently functionalized with hydrophobic alkyl groups and only dispersible in non-polar organic solvents such as toluene, hexane, and the like <xref ref-type="bibr" rid="B34">34</xref>,<xref ref-type="bibr" rid="B35">35</xref>. In order to make them amenable to bioanalytical applications, surface modification is required, to make the UCLNPs water dispersible, offering a platform for further conjugation of functional chemical groups and/or (bio)-molecules. Silica is known for its biocompatibility <xref ref-type="bibr" rid="B36">36</xref>, and silica coating of UCLNPs therefore offers an attractive way of functionalization <xref ref-type="bibr" rid="B37">37</xref>,<xref ref-type="bibr" rid="B38">38</xref>. This has already been applied to a multitude of nanoparticle systems, including gold and silver NPs <xref ref-type="bibr" rid="B39">39</xref>, magnetic NPs <xref ref-type="bibr" rid="B40">40</xref>, and quantum dots <xref ref-type="bibr" rid="B41">41</xref>. In addition, silica coating is a flexible coating technique that is applicable to both hydrophilic and hydrophobic NPs <xref ref-type="bibr" rid="B42">42</xref>,<xref ref-type="bibr" rid="B43">43</xref>,<xref ref-type="bibr" rid="B44">44</xref>. The coating process of hydrophilic NPs relies on the Stöber method, while a reverse-microemulsion method is typically used for coating hydrophobic NPs <xref ref-type="bibr" rid="B45">45</xref>.</p><p>We describe the synthesis of protein-reactive, multicolor UCLNPs. First, monodisperse, lanthanide-doped hexagonal-phase NaYF<sub>4</sub> nanoparticles were prepared, which were coated with oleic acid, as can be seen in <xref ref-type="fig" rid="FS1">Scheme 1</xref>.</p><p>In the second step, the particles were silica coated using a reverse-microemulsion method, and subsequently functionalized with an amino-reactive silanization reagent. This reagent consists of a triethoxysilane conjugated to a poly(ethylene glycol) spacer (PEG) and a carboxyl group activated with an N-hydroxysuccinimide (NHS) ester. The NHS ester of the silica-coated UCLNPs renders them highly reactive towards proteins. The protein-reactive UCLNPs exhibit multicolor luminescence emission after 980-nm laser excitation. Finally, surface plasmon resonance (SPR) studies were carried out to study the binding affinity of NHS-activated nanoparticles to proteins that previously were immobilized on a gold film.</p></sec><sec sec-type="materials|methods"><title>Materials and Methods</title><sec><title>Chemicals</title><p>Yttrium(III) chloride hexahydrate (99.99%), ytterbium(III) chloride hexahydrate (99.9%) erbium(III) chloride hexahydrate (99.9%), thulium(III) chloride hexahydrate (99.99%), ammonium fluoride (ACS reagent ≥98.0%), sodium hydroxide (reagent grade ≥98.0%) , Igepal<sup>®</sup> CO-520, tetraethyl orthosilicate (TEOS), bovine serum albumin (BSA) fraction V (purity >96% ), 16-mercapto-hexadecanoic acid (95%), N-(3-dimethylaminopropyl)-N'-ethylcarbodiimide hydrochloride (EDC) commercial grade, and sodium chloride (p.A.) were purchased from Sigma-Aldrich (<ext-link ext-link-type="uri" xlink:href="http://www.sigmaaldrich.com">www.sigmaaldrich.com</ext-link>), oleic acid (technical grade 90%) and 1-octadecene (technical grade 90%) from Alfa Aesar (<ext-link ext-link-type="uri" xlink:href="http://www.alfa.com">www.alfa.com</ext-link>), triethoxysilane poly(ethylene glycol) 2000 succinimidyl ester from Nanocs (<ext-link ext-link-type="uri" xlink:href="http://www.nanocs.com">www.nanocs.com</ext-link>), ammonia solution (32%), 2-propanol (p.A.), 2-amino-2-hydroxymethyl-propane-1,3-diol (TRIS, molecular biology grade), from Merck (<ext-link ext-link-type="uri" xlink:href="http://www.merckgroup.com">www.merckgroup.com</ext-link>), sodium hydrogen carbonate (p.A.) from Ferak (<ext-link ext-link-type="uri" xlink:href="http://www.ferak.de">www.ferak.de</ext-link>), ethanol (p.A.) from Roth (<ext-link ext-link-type="uri" xlink:href="http://www.carlroth.de">www.carlroth.de</ext-link>), and streptavidin-modified magnetic beads (PureProteome<sup>TM</sup>) from Millipore (<ext-link ext-link-type="uri" xlink:href="http://www.millipore.com">www.millipore.com</ext-link>). All other reagents and organic solvents were of the highest grade available.</p></sec><sec><title>Instrumentation</title><p>Transmission electron microscopy (TEM) was performed using a 120 kV Philips CM12 (<ext-link ext-link-type="uri" xlink:href="http://www.fei.com">www.fei.com</ext-link>) microscope. Upconversion luminescence spectra were recorded with a luminescence spectrometer (LS 50 B) from Perkin Elmer (<ext-link ext-link-type="uri" xlink:href="http://www.perkinelmer.com">www.perkinelmer.com</ext-link>), modified with a 980-nm cw laser (120 mW) from Roithner (<ext-link ext-link-type="uri" xlink:href="http://www.roithner-laser.com">www.roithner-laser.com</ext-link>) for upconversion photo-excitation. All centrifugation steps were carried out in a Hettich Universal 320 centrifuge (<ext-link ext-link-type="uri" xlink:href="http://www.hettichlab.com">www.hettichlab.com</ext-link>). Raman spectroscopy was performed using a DXR Raman microscope from Thermo Scientific (<ext-link ext-link-type="uri" xlink:href="http://www.thermoscientific.com">www.thermoscientific.com</ext-link>) with 532-nm cw laser excitation (8 mW). The Zetasizer Nano ZS from Malvern (<ext-link ext-link-type="uri" xlink:href="http://www.malvern.com">www.malvern.com</ext-link>) was used for dynamic light scattering (DLS) experiments. X-ray powder diffraction (XRD) patterns with a resolution of 0.02° (2θ) were collected using a Stoe Stadi P diffractometer (<ext-link ext-link-type="uri" xlink:href="http://www.stoe.com">www.stoe.com</ext-link>) with a Cu source (Kα radiation, λ=1.54060 Å) operating at 40 kV and 42 mA. All SPR experiments were performed with a Biosuplar 6 instrument from Mivitec GmbH, (<ext-link ext-link-type="uri" xlink:href="http://www.biosuplar.com">www.biosuplar.com</ext-link>). Typically, glass slides with a 50 nm thick gold film were used, and intensity measurements were performed at constant angle. The signal intensity was calibrated in refractive index units (RIU) with solutions of sodium chloride of different concentrations and known refractive index.</p></sec><sec><title>Synthesis of hydrophobic NaYF<sub>4</sub> nanoparticles doped with Yb/Er or Yb/Tm</title><p>Two systems of hydrophobic, lanthanide-doped NaYF<sub>4</sub> nanocrystals were prepared via a modified procedure as reported by Zhang et al. <xref ref-type="bibr" rid="B46">46</xref>. For the first system YCl<sub>3</sub>·6H<sub>2</sub>O (0.747 mmol), YbCl<sub>3</sub>·6H<sub>2</sub>O (0.25 mmol), TmCl<sub>3</sub>·6H<sub>2</sub>O (0.003 mmol) were employed. In the second system YCl<sub>3</sub>·6H<sub>2</sub>O (0.78 mmol), YbCl<sub>3</sub>·6H<sub>2</sub>O (0.20 mmol), ErCl<sub>3</sub>·6H<sub>2</sub>O (0.02 mmol) were used. The salts were dissolved in approximately 5 mL of methanol by sonication. The respective clear and optically transparent solution of rare earth chlorides in methanol was transferred into a 50 mL flask, mixed with 8 mL of oleic acid and 15 mL of 1-octadecene under an atmosphere of nitrogen and heated to 160 °C. A homogeneous, clear solution was formed after 30 minutes at 160 °C under vacuum. The reaction mixture was then cooled to room temperature and 10 mL of methanol containing NaOH (0.25 M) and NH<sub>4</sub>F (0.4 M) were added at once. The colloidal dispersion was heated to 120 °C and stirred for 30 minutes. The resulting colloid was refluxed at approximately 325 °C for 15 minutes. After cooling to room temperature, the UCLNPs were precipitated by addition of approximately 20 mL of ethanol and isolated via centrifugation at a relative centrifugal force (RCF) of 1000 g for 5 minutes. The pellet was washed several times by dispersing it in small amounts (approximately 0.5 mL) of chloroform and cyclohexane, then precipitating them by the addition of a large excess (approximately 15 mL) of ethanol and acetone. A white solid was obtained, which can be easily re-dispersed in cyclohexane to form a clear dispersion. This was used for further silica coating.</p></sec><sec><title>Silica coating of hydrophobic nanoparticles</title><p>The surface of the hydrophobic UCLNPs was coated with silica using a modified reverse-microemulsion technique <xref ref-type="bibr" rid="B36">36</xref>. 10 mg of the UCLNPs were dispersed in 10 mL of cyclohexane in a 25 mL round bottom flask. Then, 500 µL of Igepal<sup>®</sup> CO-520 and 80 µL of an aqueous ammonia solution (32%) were added. This yielded a clear and stable emulsion after 30 minutes of sonication, which was supplemented with 60 µL (0.27 mmol) of TEOS and kept for 24 hours at room temperature under magnetic stirring (600 rpm). The silica-coated UCLNPs were collected via centrifugation (RCF 3000 g for 5 minutes). Three cycles of re-dispersion and centrifugation were performed to wash the pellet with an ethanol/water mixture (1:1 v/v). The nanoparticles were filtered through a syringe filter with a pore size of 200 nm and stored in ethanol.</p></sec><sec><title>Functionalization of silica-coated nanoparticles</title><p>A fresh solution of 10 mg of triethoxysilane poly(ethylene glycol) 2000 succinimidyl ester in 500 µL of ethanol was added to a dispersion of 3 mg of silica-coated UCLNPs in 200 µL ethanol. The mixture was magnetically stirred for 2 hours at room temperature. The succinimidyl-functionalized UCLNPs were collected via centrifugation (RCF 17000 g for 10 minutes), and the unreacted materials were washed away with cold distilled water. Thereafter, the succinimidyl-functionalized UCLNPs were stored in 2-propanol at 4 °C.</p></sec><sec><title>Conjugation of succinimidyl-functionalized nanoparticles to streptavidin-modified magnetic beads</title><p>An aliquot (100 µL) of a dispersion of the magnetic beads was placed in a microcentrifuge tube. The magnetic beads were collected with a permanent magnet and washed two times with a hydrogen carbonate buffer (HCB) solution (0.1 M) adjusted to pH 9 with 1 M NaOH. After washing, the magnetic beads were dispersed in 500 µL of HCB. In parallel, 1 mg of succinimidyl-functionalized UCLNPs in 500 µL of 2-propanol was collected via centrifugation (RCF 17000 g for 10 minutes). The supernatant was discarded and 500 µL of HCB were added to the pellet. An optically transparent dispersion was obtained after sonication. The dispersions of streptavidinylated magnetic beads and protein-reactive UCLNPs were combined and magnetically stirred at room temperature for 2 hours. The streptavidin-modified magnetic bead UCLNPs conjugate was collected with a permanent magnet and washed two times with HCB.</p></sec><sec><title>Conjugation of succinimidyl-functionalized nanoparticles to bovine serum albumin</title><p>A self-assembled monolayer of a carboxy-terminated alkane thiol was prepared by immersing a gold glass slide overnight in a solution of 16-mercapto-hexadecanoic acid (400 µM) in ethanol. The protein (BSA) was bound to this surface via EDC coupling <xref ref-type="bibr" rid="B47">47</xref>. First, 20 mg of BSA were dissolved in 10 mL aqueous NaCl solution (140 mM). Second, 480 mg of EDC were added and mixed by vortexing. This solution was applied to the SPR chip. The binding of BSA to the activated carboxy groups was monitored in real time by SPR. Changes in the intensity of the reflected light were recorded at constant angle. An increase in the intensity of the SPR signal over time indicated binding of BSA. Saturation had occurred after approximately 30 minutes. The chip was washed with a solution of sodium chloride (140 mM). Again, BSA together with EDC was loaded onto the chip. Only a slight increase in the signal could be observed. After washing, the signal returned to the starting values (prior to the second immobilization). This indicates that surface modification with BSA was successful. Next, the solution in the SPR cell was changed to HCB. When the signal reached a constant value, protein-reactive UCLNPs (1 mg/mL) in HCB were added. After 80 minutes, no significant increase in the SPR signal was observed. Washing steps with hydrochloric acid (0.1 M) for 10 minutes and with HCB for 10 minutes led to a decrease in the signal by approximately 20%.</p></sec></sec><sec><title>Results and Discussion</title><p>The hydrophobic NaYF<sub>4</sub> nanoparticles doped with Yb/Er and Yb/Tm possess good monodispersity and hexagonal crystal phase. They were prepared via a modification of a known method <xref ref-type="bibr" rid="B46">46</xref>. The doping concentrations of lanthanide ions are 20/2 mol% for doping with Yb/Er, and 25/0.3 mol% for doping with Yb/Tm. Lanthanide-doped β-phase NaYF<sub>4</sub> displays superior brightness compared to other host materials <xref ref-type="bibr" rid="B48">48</xref>, which is about one order of magnitude better than comparable cubic α-phase NaYF<sub>4</sub> UCLNPs <xref ref-type="bibr" rid="B46">46</xref>,<xref ref-type="bibr" rid="B49">49</xref>. The upconversion luminescence spectra of the corresponding Yb/Er and Yb/Tm-doped samples acquired from dispersions in cyclohexane are displayed in Figure <xref ref-type="fig" rid="F1">1</xref>A. Under 980-nm cw laser excitation, the NaYF<sub>4</sub>(20%Yb/2%Er) nanocrystals yield three distinct emission peaks at 522, 541, and 655 nm. These are assigned to the <sup>4</sup>H<sub>11/2</sub> - <sup>4</sup>I<sub>15/2</sub>, <sup>4</sup>S<sub>3/2</sub> - <sup>4</sup>I<sub>15/2</sub>, and <sup>4</sup>F<sub>9/2</sub> - <sup>4</sup>I<sub>15/2</sub> transitions of Er<sup>3+</sup> ions, respectively <xref ref-type="bibr" rid="B45">45</xref>.</p><p>The spectrum of NaYF<sub>4</sub>(25%Yb/0.3%Tm) displays two blue emission peaks (450 and 475 nm), which correspond to the <sup>1</sup>D<sub>2</sub> - <sup>3</sup>F<sub>4</sub> and <sup>1</sup>G<sub>4</sub> - <sup>3</sup>H<sub>6</sub> transitions of the Tm<sup>3+</sup> ions, respectively. Additionally, there are two weaker peaks at 646 nm (<sup>3</sup>F<sub>2</sub> - <sup>3</sup>F<sub>3</sub>) and 696 nm (<sup>3</sup>H<sub>6</sub> - <sup>1</sup>G<sub>4</sub>) <xref ref-type="bibr" rid="B45">45</xref>. The predominantly blue and green emissions of optically transparent colloidal dispersions of the corresponding multicolor nanoparticles in cyclohexane upon 980-nm cw laser excitation can be seen in Figure <xref ref-type="fig" rid="F1">1</xref>B. It shall be mentioned here that the luminescence of UCLNPs strongly depends on temperature and, in fact, has been used to sense it on a nanoscale <xref ref-type="bibr" rid="B50">50</xref>.</p><p>Dispersions of the UCLNPs in cyclohexane exhibit very good colloidal stability. No sedimentation or agglomeration was found even after several weeks. Dynamic light scattering (DLS) experiments performed at 25 °C with a 632.6-nm laser and a non-invasive backscatter technique confirmed this observation. The average hydrodynamic diameter of the Yb/Er-doped nanoparticles is 34.4 nm, with a full-width at half maximum (FWHM) of 5 nm, this yielding a polydispersity index (PI) of 0.072. The respective values for the Yb/Tm-doped UCLNPs are 31.8 nm for the diameter, 5.3 nm FWHM, and a PI of 0.048. Excellent correlation statistics and fits (data not shown) were obtained using a non-negative least squares analysis algorithm.</p><p>The TEM images of the corresponding nanocrystals are shown in Figure <xref ref-type="fig" rid="F2">2</xref>. A dispersion of UCLNPs in cyclohexane was dried on a carbon-coated copper grid. The roughly spherical nanoparticles form a 2D hexagonal closed packing, as can be seen from the TEM images. This behavior may be due to van-der-Waals interaction of oleic acid (OA) molecules on the particles' surface and the solid carbon support of TEM grids. The average particle diameters as determined via TEM are 27 nm for the Yb/Er-doped sample and 25 nm for the Yb/Tm-doped sample. These results are in good agreement with the DLS data, since DLS experiments take account of the hydrodynamic diameter of the particles rather than their sheer particle size.</p><p>The results of XRD crystal phase analyses are shown in Figure <xref ref-type="fig" rid="F2">2</xref>C, and demonstrate the high crystallinity of the UCLNPs. The XRD patterns of the two samples of nanocrystals are in good agreement with the standard pattern of β-NaYF<sub>4</sub> (ICDD PDF 16-334). Raman spectroscopy was used to characterize the phonon bands of the NaYF<sub>4</sub> nanocrystals (Figure <xref ref-type="fig" rid="F3">3</xref>A). They are clearly visible and distinct between 225 - 450 cm<sup>-1</sup>. The weighed average of the phonon modes is 304 cm<sup>-1</sup> for the Yb/Er-doped sample, and 320 cm<sup>-1</sup> for the Yb/Tm-doped sample. This phonon energy is considerably lower than that of comparable fluoride host lattices such as LiYF<sub>4</sub> (570 cm<sup>-1</sup>) <xref ref-type="bibr" rid="B51">51</xref>,<xref ref-type="bibr" rid="B52">52</xref>. Additionally, it is predictably lower than that of bulk, un-doped NaYF<sub>4</sub> (360 cm<sup>-1</sup>) <xref ref-type="bibr" rid="B53">53</xref> due to the modified phonon density of states <xref ref-type="bibr" rid="B54">54</xref>. Oleic acid has a distinct Raman spectrum in the 2830 - 2960 cm<sup>-1</sup> region where CH<sub>2</sub> stretching modes are observed. These are particularly susceptible to thermal changes, and slight spectral variations are expected from sample to sample for this reason <xref ref-type="bibr" rid="B55">55</xref>. The Raman spectra for this spectral region are shown in Figure <xref ref-type="fig" rid="F3">3</xref>B. Additional evidence of the presence of OA is exhibited by the strong CH<sub>2</sub> scissoring modes in the 1438 - 1456 cm<sup>‑1</sup> region (not shown here). Other (but much weaker) Raman peaks for oleic acid can be observed in the 600 - 1800 cm<sup>-1</sup> region.</p><p>The hydrophobic, oleic acid-coated UCLNPs were then silica coated via a reverse-microemulsion technique according to a modified literature method <xref ref-type="bibr" rid="B36">36</xref>. This makes them water dispersible and biocompatible, which is a prerequisite for almost any bioapplications. The TEM images in Figure <xref ref-type="fig" rid="F4">4</xref> demonstrate that this technique yields a thin and uniform silica coating on the hydrophobic nanoparticles. The formation of a silica shell can be clearly deduced from the TEM images, because silica exhibits an electron optical contrast which is quite different from that of rare earth-doped nanocrystals. The size of the Yb/Er-doped nanoparticles increased from 27 to 38 nm after silica coating, implying a shell thickness of approximately 5 nm. The diameter of the respective Yb/Tm-doped particles increased from 25 to 38 nm, and this corresponds to a shell thickness of about 6 nm.</p><p>The normalized upconversion luminescence spectra of Yb/Er and Yb/Tm-doped, silica coated, multicolor particles dispersed in deionized water solution are shown in Figure <xref ref-type="fig" rid="F4">4</xref>C. The silica-coated nanocrystals also exhibit a Raman peak at 1381 cm<sup>-1</sup> as shown in Figure <xref ref-type="fig" rid="F4">4</xref>D. This band is not present for the uncoated nanoparticles, and is likely to be due to the Si-CH<sub>2</sub> scissoring mode <xref ref-type="bibr" rid="B56">56</xref>.</p><p>In the next step, a silane reagent with a PEG2000 spacer and activated as an N-hydroxysuccinimide (NHS) ester was covalently bound to the surface via a silanization technique <xref ref-type="bibr" rid="B57">57</xref>. The PEG spacer is benefical in that it can prevent agglomeration, reduce unspecific binding, and improve solubility in water. The NHS ester groups render the silica-coated UCLNPs highly reactive towards proteins, as shown by labeling of streptavidin-modified magnetic beads.</p><p>We have attempted to calculate the number of NHS groups on a nanocrystal. In order to do so, the following assumptions have been made: (a) The average radius of the nanocrystals is 19 nm.</p><p>(b) The average radius of a (spherical) PEG 2000 molecule is approximately 1.5 nm <xref ref-type="bibr" rid="B58">58</xref> The volume of a spherical nanocrystal with a radius of 19 nm can be calculated to be 29 µm³, and the volume of a spherical PEG 2000 molecule with a radius of 1.5 nm to be 14 nm³. The total volume of a spherical nanocrystal loaded with PEG 2000 is 45 µm³. The number of PEG 2000 molecules can be calculated if the difference (45 µm³ - 29 µm³) is divided by 14 nm³ and yields about 1100 NHS groups per (spherical) silica coated nanocrystal. We have to stress here that this is a rough number only and also presume that the number of NHS groups per nanocrystal can be governed (reduced) by using mixtures of PEG2000-NHS reagent and PEG2000-modified silyl reagent (without NHS groups), but this has not been verified experimentally.</p><p>Two sets of experiments were carried out. In a first (positive control) experiment, the streptavidinylated magnetic beads were mixed with UCLNP NHS esters in a conjugation buffer (HCB) of pH 9. After 2 hours at room temperature, the magnetic beads were collected with a permanent magnet and washed with HCB. The collected spot of streptavidin-modified magnetic beads was identified by its upconversion luminescence upon 980-nm cw laser excitation (Figure <xref ref-type="fig" rid="F5">5</xref>A). This proves that the protein-reactive multicolor UCLNPs bind to streptavidin. In a second (negative control) experiment, the UCLNP NHS esters were previously deactivated by reacting them with the amino groups of TRIS buffer solution (2 mM, pH 8.5) overnight at room temperature. The first experiment was then repeated with the deactivated nanoparticles. Indeed, the upconversion luminescence of the collected cluster of streptavidin-modified magnetic beads was not observed. This fact proves that the deactivated particles are not bound to the streptavidinylated magnetic beads. On the other hand, a dispersion of the unreactive particles also display upconversion emission upon 980-nm cw laser excitation (Figure <xref ref-type="fig" rid="F5">5</xref>B).</p><p>In order to quantitatively verify the functionality of protein-reactive nanoparticles, their binding to surface-immobilized BSA was monitored by SPR measurements in real time. SPR provides a well-known label free method to study interaction of biomolecules on thin gold films <xref ref-type="bibr" rid="B59">59</xref>. We coated the thin gold surface with a monolayer of a carboxy-terminated alkanethiol. The protein was immobilized via EDC coupling onto this surface. The binding of protein-reactive NPs to BSA was studied by measuring the shift in the surface plasmon resonance. Therefore, one can either measure the angle of minimum reflection of the light as a function of time, or monitor the time-dependent change in the intensity of the reflected light at a constant angle of incidence. The kinetics for binding of the nanoparticles to the protein layer can be seen in Figure <xref ref-type="fig" rid="F5">5</xref>C. Both the protein-reactive and the deactivated UCLNPs bind to BSA. After washing with 0.1 mM hydrochloric acid for 10 minutes and then with hydrogen carbonate buffer for another 10 minutes, it can be clearly seen that the protein-reactive particles still bind quite strongly. Some deactivated particles also bind due to non-specific binding. On the other hand, when using nanoparticles with a silica shell without NHS groups, we also see unspecific binding, but the particles can be simply washed off with buffer.</p></sec><sec sec-type="conclusions"><title>Conclusion</title><p>We report here on the preparation of monodisperse, multicolor UCLNPs with controlled diameters of ~26 nm using a modified solvothermal method. In order to make them amenable to bioanalytical applications, surface modification was performed by first depositing a thin silica shell (~5 nm thick) on the hydrophobic nanoparticles, this followed by coating it with a poly(ethylene glycol) spacer carrying N-hydroxysuccinimide groups. The resulting particles form stable dispersions in aqueous solution and are highly reactive towards proteins such as streptavidin and bovine serum albumin. Such amino-reactive labels form an attractive alternative to thiol-reactive UCLNPs <xref ref-type="bibr" rid="B60">60</xref>. Specifically, the reactive UCLNPs were conjugated to streptavidin-modified magnetic beads. The magnetic beads labeled with streptavidinylated UCLNPs were separated by magnetic force and displayed upconversion luminescence upon 980-nm laser excitation. We believe that such amino-reactive multicolor nanoparticles can be employed as luminescent labels for various kinds of (organic) amines, biogenic amines, proteins, or amino-modified oligomers. Labeled proteins have numerous applications such as in immunoassays, enzymatic assays, and in imaging. 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position="float"><label>Figure 1</label><caption><p>(<bold>A</bold>) Normalized upconversion luminescence spectra of Yb/Tm (dotted blue line) and Yb/Er-doped (solid green line) multicolor β-NaYF<sub>4</sub> nanocrystals. The UCLNPs were dispersed in cyclohexane (1 mg/mL). (<bold>B</bold>) Digital photograph of optically transparent colloidal dispersions of corresponding UCLNPs (1 mg/mL) in cyclohexane. The predominantly blue and green emissions of Yb/Tm and Yb/Er-doped UCLNPs upon 980-nm cw laser excitation can easily be seen by the bare eye.</p></caption><graphic xlink:href="thnov03p0239g02"></graphic></fig><fig id="F2" position="float"><label>Figure 2</label><caption><p>TEM images of NaYF<sub>4</sub>(20%Yb/2%Er) (<bold>A</bold>) and NaYF<sub>4</sub>(25%Yb/0.3%Tm) (<bold>B</bold>) upconversion luminescent nanoparticles, respectively. Scale bar: 100 nm. Graph (<bold>C</bold>) shows the corresponding XRD patterns and the standard XRD pattern of β-NaYF<sub>4</sub> (ICDD PDF 16-334).</p></caption><graphic xlink:href="thnov03p0239g03"></graphic></fig><fig id="F3" position="float"><label>Figure 3</label><caption><p>Raman spectra of UCLNPs focusing on the NaYF<sub>4</sub> phonon region (<bold>A</bold>), and the CH<sub>2</sub> stretching corresponding to the functionalized OA (<bold>B</bold>). The dotted blue line corresponds to the Yb/Tm-doped sample and the solid green line to the Yb/Er-doped UCLNPs. The Raman spectrum of pure, non-functionalized OA (solid grey line) is shown in (<bold>B</bold>) for comparison. The slight peak shifts and difference in peak widths between the non-functionalized OA and the OA-functionalized UCLNPs indicate that OA is likely to be bound to the surface of UCLNPs.</p></caption><graphic xlink:href="thnov03p0239g04"></graphic></fig><fig id="F4" position="float"><label>Figure 4</label><caption><p>TEM images of silica-coated upconversion luminescent nanoparticles (UCLNPs) of the type NaYF<sub>4</sub>(20%Yb/2%Er)@silica (<bold>A</bold>) and of the type NaYF<sub>4</sub>(25%Yb/0.3%Tm)@silica (<bold>B</bold>) in water (1 mg/mL) before functionalization with PEG2000-NHS. The sample was prepared by dropping an aliquot of approximately 2 µL of silica-coated UCLNPs dispersion onto the surface of a carbon-coated copper grid. Scale bar: 100 nm. The normalized upconversion luminescence spectra of silica-coated Yb/Tm (dotted blue line) and Yb/Er-doped, β-NaYF<sub>4</sub> nanocrystals (solid green line) are shown in (<bold>C</bold>). The Raman spectra of UCLNPs with silica (solid lines) and without silica (dotted lines) are shown in (<bold>D</bold>). The same conditions were maintained in all experiments.</p></caption><graphic xlink:href="thnov03p0239g05"></graphic></fig><fig id="F5" position="float"><label>Figure 5</label><caption><p>(<bold>A; </bold>top) Digital photograph of the protein-reactive upconversion luminescent nanoparticles (UCLNPs) bound to streptavidin-modified magnetic beads (<bold>2</bold>), collected with a permanent magnet (<bold>1</bold>) upon 980-nm cw laser (<bold>3</bold>) excitation (280 mW) in a hydrogen carbonate buffer. (<bold>B</bold>; top) Photograph illustrating that UCLNP NHS esters that were deactivated by reaction with Tris buffer and dispersed in a hydrogen carbonate buffer do not bind to streptavidinylated magnetic beads (<bold>2</bold>). (<bold>C;</bold> bottom) SPR results showing the unspecific binding of deactivated UCLNPs (dotted red line) and specific binding of protein-reactive UCLNPs (solid black line) to BSA immobilized on a gold substrate. The curves show the addition of the respective UCLNPs in a hydrogen carbonate buffer (<bold>a</bold>), and the washing steps with hydrochloric acid (0.1 M, <bold>b</bold>), and hydrogen carbonate buffer solution (<bold>c</bold>).</p></caption><graphic xlink:href="thnov03p0239g06"></graphic></fig></floats-group></pmc></record>Pour manipuler ce document sous Unix (Dilib)
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