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<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">Multiphoton Imaging of Upconverting Lanthanide Nanoparticles in Three Dimensional Models of Cancer</title>
<author><name sortKey="Gainer, Christian F" sort="Gainer, Christian F" uniqKey="Gainer C" first="Christian F." last="Gainer">Christian F. Gainer</name>
<affiliation><nlm:aff id="A1">Dept. of Biomedical Engineering, University of Arizona, 1657 E Helen St, Tucson, AZ, USA 85721</nlm:aff>
</affiliation>
</author>
<author><name sortKey="Romanowski, Marek" sort="Romanowski, Marek" uniqKey="Romanowski M" first="Marek" last="Romanowski">Marek Romanowski</name>
<affiliation><nlm:aff id="A1">Dept. of Biomedical Engineering, University of Arizona, 1657 E Helen St, Tucson, AZ, USA 85721</nlm:aff>
</affiliation>
</author>
</titleStmt>
<publicationStmt><idno type="wicri:source">PMC</idno>
<idno type="pmid">24353385</idno>
<idno type="pmc">3864927</idno>
<idno type="url">http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3864927</idno>
<idno type="RBID">PMC:3864927</idno>
<idno type="doi">10.1117/12.2002551</idno>
<date when="2013">2013</date>
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<sourceDesc><biblStruct><analytic><title xml:lang="en" level="a" type="main">Multiphoton Imaging of Upconverting Lanthanide Nanoparticles in Three Dimensional Models of Cancer</title>
<author><name sortKey="Gainer, Christian F" sort="Gainer, Christian F" uniqKey="Gainer C" first="Christian F." last="Gainer">Christian F. Gainer</name>
<affiliation><nlm:aff id="A1">Dept. of Biomedical Engineering, University of Arizona, 1657 E Helen St, Tucson, AZ, USA 85721</nlm:aff>
</affiliation>
</author>
<author><name sortKey="Romanowski, Marek" sort="Romanowski, Marek" uniqKey="Romanowski M" first="Marek" last="Romanowski">Marek Romanowski</name>
<affiliation><nlm:aff id="A1">Dept. of Biomedical Engineering, University of Arizona, 1657 E Helen St, Tucson, AZ, USA 85721</nlm:aff>
</affiliation>
</author>
</analytic>
<series><title level="j">Proceedings of SPIE--the International Society for Optical Engineering</title>
<idno type="ISSN">0277-786X</idno>
<idno type="eISSN">1996-756X</idno>
<imprint><date when="2013">2013</date>
</imprint>
</series>
</biblStruct>
</sourceDesc>
</fileDesc>
<profileDesc><textClass></textClass>
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<front><div type="abstract" xml:lang="en"><p id="P1">While upconverting lanthanide nanoparticles have numerous advantages over other exogenous contrast agents used in scanned multiphoton imaging, their long luminescence lifetimes cause images collected with non-descanned detection to be greatly blurred. We demonstrate herein the use of Richardson-Lucy deconvolution to deblur luminescence images obtained via multiphoton scanning microscopy. Images were taken of three dimensional models of colon and ovarian cancer following incubation with NaYF<sub>4</sub>
:Yb,Er nanoparticles functionalized with an antibody for EGFR and folic acid respectively. Following deconvolution, images had a lateral resolution on par with the optimal performance of the imaging system used, ~1.2 μm, and an axial resolution of ~5 μm. Due to the relatively high multiphoton excitation efficiency of these nanoparticles, it is possible to follow binding of individual particles in tissue. In addition, their extreme photostability allows for prolonged imaging without significant loss in luminescence signal. With these advantageous properties in mind, we also discuss the potential application of upconverting lanthanide nanoparticles for tracking of specific, cancer relevant receptors in tissue.</p>
</div>
</front>
</TEI>
<pmc article-type="research-article"><pmc-comment>The publisher of this article does not allow downloading of the full text in XML form.</pmc-comment>
<pmc-dir>properties manuscript</pmc-dir>
<front><journal-meta><journal-id journal-id-type="nlm-journal-id">101524122</journal-id>
<journal-id journal-id-type="pubmed-jr-id">37467</journal-id>
<journal-id journal-id-type="nlm-ta">Proc SPIE Int Soc Opt Eng</journal-id>
<journal-id journal-id-type="iso-abbrev">Proc SPIE Int Soc Opt Eng</journal-id>
<journal-title-group><journal-title>Proceedings of SPIE--the International Society for Optical Engineering</journal-title>
</journal-title-group>
<issn pub-type="ppub">0277-786X</issn>
<issn pub-type="epub">1996-756X</issn>
</journal-meta>
<article-meta><article-id pub-id-type="pmid">24353385</article-id>
<article-id pub-id-type="pmc">3864927</article-id>
<article-id pub-id-type="doi">10.1117/12.2002551</article-id>
<article-id pub-id-type="manuscript">NIHMS489455</article-id>
<article-categories><subj-group subj-group-type="heading"><subject>Article</subject>
</subj-group>
</article-categories>
<title-group><article-title>Multiphoton Imaging of Upconverting Lanthanide Nanoparticles in Three Dimensional Models of Cancer</article-title>
</title-group>
<contrib-group><contrib contrib-type="author"><name><surname>Gainer</surname>
<given-names>Christian F.</given-names>
</name>
<xref ref-type="aff" rid="A1">a</xref>
</contrib>
<contrib contrib-type="author"><name><surname>Romanowski</surname>
<given-names>Marek</given-names>
</name>
<xref ref-type="corresp" rid="CR1">*</xref>
<xref ref-type="aff" rid="A1">a</xref>
</contrib>
</contrib-group>
<aff id="A1"><label>a</label>
Dept. of Biomedical Engineering, University of Arizona, 1657 E Helen St, Tucson, AZ, USA 85721</aff>
<author-notes><corresp id="CR1"><label>*</label>
<email>marekrom@email.arizona.edu</email>
; phone 1 520 626-1578; fax 1 520 626-4824</corresp>
</author-notes>
<pub-date pub-type="nihms-submitted"><day>27</day>
<month>6</month>
<year>2013</year>
</pub-date>
<pub-date pub-type="ppub"><day>22</day>
<month>2</month>
<year>2013</year>
</pub-date>
<pub-date pub-type="pmc-release"><day>16</day>
<month>12</month>
<year>2013</year>
</pub-date>
<volume>8595</volume>
<fpage>85950O</fpage>
<lpage></lpage>
<pmc-comment>elocation-id from pubmed: 10.1117/12.2002551</pmc-comment>
<abstract><p id="P1">While upconverting lanthanide nanoparticles have numerous advantages over other exogenous contrast agents used in scanned multiphoton imaging, their long luminescence lifetimes cause images collected with non-descanned detection to be greatly blurred. We demonstrate herein the use of Richardson-Lucy deconvolution to deblur luminescence images obtained via multiphoton scanning microscopy. Images were taken of three dimensional models of colon and ovarian cancer following incubation with NaYF<sub>4</sub>
:Yb,Er nanoparticles functionalized with an antibody for EGFR and folic acid respectively. Following deconvolution, images had a lateral resolution on par with the optimal performance of the imaging system used, ~1.2 μm, and an axial resolution of ~5 μm. Due to the relatively high multiphoton excitation efficiency of these nanoparticles, it is possible to follow binding of individual particles in tissue. In addition, their extreme photostability allows for prolonged imaging without significant loss in luminescence signal. With these advantageous properties in mind, we also discuss the potential application of upconverting lanthanide nanoparticles for tracking of specific, cancer relevant receptors in tissue.</p>
</abstract>
<kwd-group><kwd>upconversion</kwd>
<kwd>lanthanide</kwd>
<kwd>nanoparticle</kwd>
<kwd>2p</kwd>
<kwd>microscopy</kwd>
<kwd>erbium</kwd>
</kwd-group>
</article-meta>
</front>
</pmc>
</record>
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