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<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">A Modified Alderman-Grant Coil Makes Possible an Efficient Cross-Coil Probe for High Field Solid-state NMR of Lossy Biological Samples</title><author><name sortKey="Grant, Christopher V" sort="Grant, Christopher V" uniqKey="Grant C" first="Christopher V." last="Grant">Christopher V. Grant</name><affiliation><nlm:aff id="A1"> Department of Chemistry and Biochemistry, University of California, San Diego, 9500 Gilman Drive, La Jolla, CA 92093-0307</nlm:aff></affiliation></author><author><name sortKey="Yang, Yuan" sort="Yang, Yuan" uniqKey="Yang Y" first="Yuan" last="Yang">Yuan Yang</name><affiliation><nlm:aff id="A1"> Department of Chemistry and Biochemistry, University of California, San Diego, 9500 Gilman Drive, La Jolla, CA 92093-0307</nlm:aff></affiliation></author><author><name sortKey="Glibowicka, Mira" sort="Glibowicka, Mira" uniqKey="Glibowicka M" first="Mira" last="Glibowicka">Mira Glibowicka</name><affiliation><nlm:aff id="A2"> Division of Molecular Structure and Function, Research Institute, Hospital for Sick Children, Toronto M5G 1X8, Ontario, Canada</nlm:aff></affiliation></author><author><name sortKey="Wu, Chin H" sort="Wu, Chin H" uniqKey="Wu C" first="Chin H." last="Wu">Chin H. Wu</name><affiliation><nlm:aff id="A1"> Department of Chemistry and Biochemistry, University of California, San Diego, 9500 Gilman Drive, La Jolla, CA 92093-0307</nlm:aff></affiliation></author><author><name sortKey="Park, Sang Ho" sort="Park, Sang Ho" uniqKey="Park S" first="Sang Ho" last="Park">Sang Ho Park</name><affiliation><nlm:aff id="A1"> Department of Chemistry and Biochemistry, University of California, San Diego, 9500 Gilman Drive, La Jolla, CA 92093-0307</nlm:aff></affiliation></author><author><name sortKey="Deber, Charles M" sort="Deber, Charles M" uniqKey="Deber C" first="Charles M." last="Deber">Charles M. Deber</name><affiliation><nlm:aff id="A2"> Division of Molecular Structure and Function, Research Institute, Hospital for Sick Children, Toronto M5G 1X8, Ontario, Canada</nlm:aff></affiliation></author><author><name sortKey="Opella, Stanley J" sort="Opella, Stanley J" uniqKey="Opella S" first="Stanley J." last="Opella">Stanley J. Opella</name><affiliation><nlm:aff id="A1"> Department of Chemistry and Biochemistry, University of California, San Diego, 9500 Gilman Drive, La Jolla, CA 92093-0307</nlm:aff></affiliation></author></titleStmt><publicationStmt><idno type="wicri:source">PMC</idno><idno type="pmid">19733108</idno><idno type="pmc">2800166</idno><idno type="url">http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2800166</idno><idno type="RBID">PMC:2800166</idno><idno type="doi">10.1016/j.jmr.2009.08.009</idno><date when="2009">2009</date><idno type="wicri:Area/Pmc/Corpus">000240</idno><idno type="wicri:explorRef" wicri:stream="Pmc" wicri:step="Corpus" wicri:corpus="PMC">000240</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en" level="a" type="main">A Modified Alderman-Grant Coil Makes Possible an Efficient Cross-Coil Probe for High Field Solid-state NMR of Lossy Biological Samples</title><author><name sortKey="Grant, Christopher V" sort="Grant, Christopher V" uniqKey="Grant C" first="Christopher V." last="Grant">Christopher V. Grant</name><affiliation><nlm:aff id="A1"> Department of Chemistry and Biochemistry, University of California, San Diego, 9500 Gilman Drive, La Jolla, CA 92093-0307</nlm:aff></affiliation></author><author><name sortKey="Yang, Yuan" sort="Yang, Yuan" uniqKey="Yang Y" first="Yuan" last="Yang">Yuan Yang</name><affiliation><nlm:aff id="A1"> Department of Chemistry and Biochemistry, University of California, San Diego, 9500 Gilman Drive, La Jolla, CA 92093-0307</nlm:aff></affiliation></author><author><name sortKey="Glibowicka, Mira" sort="Glibowicka, Mira" uniqKey="Glibowicka M" first="Mira" last="Glibowicka">Mira Glibowicka</name><affiliation><nlm:aff id="A2"> Division of Molecular Structure and Function, Research Institute, Hospital for Sick Children, Toronto M5G 1X8, Ontario, Canada</nlm:aff></affiliation></author><author><name sortKey="Wu, Chin H" sort="Wu, Chin H" uniqKey="Wu C" first="Chin H." last="Wu">Chin H. Wu</name><affiliation><nlm:aff id="A1"> Department of Chemistry and Biochemistry, University of California, San Diego, 9500 Gilman Drive, La Jolla, CA 92093-0307</nlm:aff></affiliation></author><author><name sortKey="Park, Sang Ho" sort="Park, Sang Ho" uniqKey="Park S" first="Sang Ho" last="Park">Sang Ho Park</name><affiliation><nlm:aff id="A1"> Department of Chemistry and Biochemistry, University of California, San Diego, 9500 Gilman Drive, La Jolla, CA 92093-0307</nlm:aff></affiliation></author><author><name sortKey="Deber, Charles M" sort="Deber, Charles M" uniqKey="Deber C" first="Charles M." last="Deber">Charles M. Deber</name><affiliation><nlm:aff id="A2"> Division of Molecular Structure and Function, Research Institute, Hospital for Sick Children, Toronto M5G 1X8, Ontario, Canada</nlm:aff></affiliation></author><author><name sortKey="Opella, Stanley J" sort="Opella, Stanley J" uniqKey="Opella S" first="Stanley J." last="Opella">Stanley J. Opella</name><affiliation><nlm:aff id="A1"> Department of Chemistry and Biochemistry, University of California, San Diego, 9500 Gilman Drive, La Jolla, CA 92093-0307</nlm:aff></affiliation></author></analytic><series><title level="j">Journal of magnetic resonance (San Diego, Calif. : 1997)</title><idno type="ISSN">1090-7807</idno><idno type="eISSN">1096-0856</idno><imprint><date when="2009">2009</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en"><p id="P2">The design, construction, and performance of a cross-coil double-resonance probe for solid-state NMR experiments on lossy biological samples at high magnetic fields are described. The outer coil is a Modified Alderman-Grant Coil (MAGC) tuned to the <sup>1</sup>H frequency. The inner coil consists of a multi-turn solenoid coil that produces a B<sub>1</sub> field orthogonal to that of the outer coil. This results in a compact nested cross-coil pair with the inner solenoid coil tuned to the low frequency detection channel. This design has several advantages over multiple-tuned solenoid coil probes, since RF heating from the <sup>1</sup>H channel is substantially reduced, it can be tuned for samples with a wide range of dielectric constants, and the simplified circuit design and high inductance inner coil provides excellent sensitivity. The utility of this probe is demonstrated on two electrically lossy samples of membrane proteins in phospholipid bilayers (bicelles) that are particularly difficult for conventional NMR probes. The 72-residue polypeptide embedding the transmembrane helices 3 and 4 of the Cystic Fibrosis Transmembrane Conductance Regulator (CFTR) (residues 194 – 241) requires a high salt concentration in order to be successfully reconstituted in phospholipid bicelles. A second application is to paramagnetic relaxation enhancement applied to the membrane-bound form of Pf1 coat protein in phospholipid bicelles where the resistance to sample heating enables high duty cycle solid-state NMR experiments to be performed.</p></div></front></TEI><pmc article-type="research-article" xml:lang="EN"><pmc-comment>The publisher of this article does not allow downloading of the full text in XML form.</pmc-comment>
<pmc-dir>properties manuscript</pmc-dir>
<front><journal-meta><journal-id journal-id-type="nlm-journal-id">9707935</journal-id><journal-id journal-id-type="pubmed-jr-id">20597</journal-id><journal-id journal-id-type="nlm-ta">J Magn Reson</journal-id><journal-title>Journal of magnetic resonance (San Diego, Calif. : 1997)</journal-title><issn pub-type="ppub">1090-7807</issn><issn pub-type="epub">1096-0856</issn></journal-meta><article-meta><article-id pub-id-type="pmid">19733108</article-id><article-id pub-id-type="pmc">2800166</article-id><article-id pub-id-type="doi">10.1016/j.jmr.2009.08.009</article-id><article-id pub-id-type="manuscript">NIHMS143819</article-id><article-categories><subj-group subj-group-type="heading"><subject>Article</subject></subj-group></article-categories><title-group><article-title>A Modified Alderman-Grant Coil Makes Possible an Efficient Cross-Coil Probe for High Field Solid-state NMR of Lossy Biological Samples</article-title></title-group><contrib-group><contrib contrib-type="author"><name><surname>Grant</surname><given-names>Christopher V.</given-names></name><xref rid="A1" ref-type="aff">a</xref></contrib><contrib contrib-type="author"><name><surname>Yang</surname><given-names>Yuan</given-names></name><xref rid="A1" ref-type="aff">a</xref></contrib><contrib contrib-type="author"><name><surname>Glibowicka</surname><given-names>Mira</given-names></name><xref rid="A2" ref-type="aff">b</xref></contrib><contrib contrib-type="author"><name><surname>Wu</surname><given-names>Chin H.</given-names></name><xref rid="A1" ref-type="aff">a</xref></contrib><contrib contrib-type="author"><name><surname>Park</surname><given-names>Sang Ho</given-names></name><xref rid="A1" ref-type="aff">a</xref></contrib><contrib contrib-type="author"><name><surname>Deber</surname><given-names>Charles M.</given-names></name><xref rid="A2" ref-type="aff">b</xref></contrib><contrib contrib-type="author"><name><surname>Opella</surname><given-names>Stanley J.</given-names></name><xref rid="A1" ref-type="aff">a</xref><xref rid="FN1" ref-type="author-notes">*</xref></contrib></contrib-group><aff id="A1"><label>a</label> Department of Chemistry and Biochemistry, University of California, San Diego, 9500 Gilman Drive, La Jolla, CA 92093-0307</aff><aff id="A2"><label>b</label> Division of Molecular Structure and Function, Research Institute, Hospital for Sick Children, Toronto M5G 1X8, Ontario, Canada</aff><author-notes><corresp id="FN1"><label>*</label>Corresponding Author. <email>sopella@ucsd.edu</email>, 858.822.4820</corresp></author-notes><pub-date pub-type="nihms-submitted"><day>8</day><month>9</month><year>2009</year></pub-date><pub-date pub-type="epub"><day>15</day><month>8</month><year>2009</year></pub-date><pub-date pub-type="ppub"><month>11</month><year>2009</year></pub-date><pub-date pub-type="pmc-release"><day>1</day><month>11</month><year>2010</year></pub-date><volume>201</volume><issue>1</issue><fpage>87</fpage><lpage>92</lpage><abstract><p id="P2">The design, construction, and performance of a cross-coil double-resonance probe for solid-state NMR experiments on lossy biological samples at high magnetic fields are described. The outer coil is a Modified Alderman-Grant Coil (MAGC) tuned to the <sup>1</sup>H frequency. The inner coil consists of a multi-turn solenoid coil that produces a B<sub>1</sub> field orthogonal to that of the outer coil. This results in a compact nested cross-coil pair with the inner solenoid coil tuned to the low frequency detection channel. This design has several advantages over multiple-tuned solenoid coil probes, since RF heating from the <sup>1</sup>H channel is substantially reduced, it can be tuned for samples with a wide range of dielectric constants, and the simplified circuit design and high inductance inner coil provides excellent sensitivity. The utility of this probe is demonstrated on two electrically lossy samples of membrane proteins in phospholipid bilayers (bicelles) that are particularly difficult for conventional NMR probes. The 72-residue polypeptide embedding the transmembrane helices 3 and 4 of the Cystic Fibrosis Transmembrane Conductance Regulator (CFTR) (residues 194 – 241) requires a high salt concentration in order to be successfully reconstituted in phospholipid bicelles. A second application is to paramagnetic relaxation enhancement applied to the membrane-bound form of Pf1 coat protein in phospholipid bicelles where the resistance to sample heating enables high duty cycle solid-state NMR experiments to be performed.</p></abstract><kwd-group><kwd>solid-state NMR</kwd><kwd>probe</kwd><kwd>sample heating</kwd><kwd>Alderman-Grant coil</kwd><kwd>membrane proteins</kwd></kwd-group><contract-num rid="GM1">R01 GM075877-05
||GM</contract-num><contract-num rid="GM1">R01 GM066978-06
||GM</contract-num><contract-num rid="EB1">P41 EB002031-16
||EB</contract-num><contract-sponsor id="GM1">National Institute of General Medical Sciences : NIGMS</contract-sponsor><contract-sponsor id="EB1">National Institute of Biomedical Imaging and Bioengineering : NIBIB</contract-sponsor></article-meta></front></pmc></record>Pour manipuler ce document sous Unix (Dilib)
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