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<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">Highly Sensitive and Selective Label-Free Optical Detection of Mercuric Ions Using Photon Upconverting Nanoparticles</title><author><name sortKey="Kumar, Manoj" sort="Kumar, Manoj" uniqKey="Kumar M" first="Manoj" last="Kumar">Manoj Kumar</name></author><author><name sortKey="Zhang, Peng" sort="Zhang, Peng" uniqKey="Zhang P" first="Peng" last="Zhang">Peng Zhang</name></author></titleStmt><publicationStmt><idno type="wicri:source">PMC</idno><idno type="pmid">20456935</idno><idno type="pmc">2880226</idno><idno type="url">http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2880226</idno><idno type="RBID">PMC:2880226</idno><idno type="doi">10.1016/j.bios.2010.03.038</idno><date when="2010">2010</date><idno type="wicri:Area/Pmc/Corpus">000234</idno><idno type="wicri:explorRef" wicri:stream="Pmc" wicri:step="Corpus" wicri:corpus="PMC">000234</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en" level="a" type="main">Highly Sensitive and Selective Label-Free Optical Detection of Mercuric Ions Using Photon Upconverting Nanoparticles</title><author><name sortKey="Kumar, Manoj" sort="Kumar, Manoj" uniqKey="Kumar M" first="Manoj" last="Kumar">Manoj Kumar</name></author><author><name sortKey="Zhang, Peng" sort="Zhang, Peng" uniqKey="Zhang P" first="Peng" last="Zhang">Peng Zhang</name></author></analytic><series><title level="j">Biosensors & bioelectronics</title><idno type="ISSN">0956-5663</idno><idno type="eISSN">1873-4235</idno><imprint><date when="2010">2010</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en"><p id="P1">We demonstrate a fluorescence-based, label-free detection scheme that reports the presence of Hg(II) ion using photon upconverting nanoparticles. A single-stranded DNA containing a number of thymine bases to be used as the Hg<sup>2+</sup>-capturing element is covalently attached to the photon upconverting NaYF<sub>4</sub>:Yb<sup>3+</sup>,Tm<sup>3+</sup> nanoparticles. Under the illumination of 980 nm laser, energy transfer takes place between the NaYF<sub>4</sub>:Yb<sup>3+</sup>,Tm<sup>3+</sup> nanoparticles as the donor and SYBR green I, a DNA intercalating dye, as the acceptor. By monitoring the ratio of the acceptor emission to the donor emission, we can quantitatively detect the presence of the mercuric ions with a directly observed detection limit of 0.06 nM. The remarkably high signal-to-noise ratio of photon upconverting particles leads to very high sensitivity and specificity without the need of fluorophore labeling. The sensor also does not suffer from photobleaching.</p></div></front></TEI><pmc article-type="research-article" xml:lang="EN"><pmc-comment>The publisher of this article does not allow downloading of the full text in XML form.</pmc-comment>
<pmc-dir>properties manuscript</pmc-dir>
<front><journal-meta><journal-id journal-id-type="nlm-journal-id">9001289</journal-id><journal-id journal-id-type="pubmed-jr-id">1537</journal-id><journal-id journal-id-type="nlm-ta">Biosens Bioelectron</journal-id><journal-title>Biosensors & bioelectronics</journal-title><issn pub-type="ppub">0956-5663</issn><issn pub-type="epub">1873-4235</issn></journal-meta><article-meta><article-id pub-id-type="pmid">20456935</article-id><article-id pub-id-type="pmc">2880226</article-id><article-id pub-id-type="doi">10.1016/j.bios.2010.03.038</article-id><article-id pub-id-type="manuscript">NIHMS194490</article-id><article-categories><subj-group subj-group-type="heading"><subject>Article</subject></subj-group></article-categories><title-group><article-title>Highly Sensitive and Selective Label-Free Optical Detection of Mercuric Ions Using Photon Upconverting Nanoparticles</article-title></title-group><contrib-group><contrib contrib-type="author"><name><surname>Kumar</surname><given-names>Manoj</given-names></name></contrib><contrib contrib-type="author"><name><surname>Zhang</surname><given-names>Peng</given-names></name><xref ref-type="corresp" rid="CR1">*</xref></contrib><aff id="A1">Laboratory of Nanomaterial Science, Department of Chemistry, New Mexico Tech, Socorro, NM 87801</aff></contrib-group><author-notes><corresp id="CR1"><label>*</label>Corresponding author: Peng Zhang, Tel: 575-835-6192. Fax: 575-835-5364. <email>pzhang@nmt.edu</email></corresp></author-notes><pub-date pub-type="nihms-submitted"><day>22</day><month>4</month><year>2010</year></pub-date><pub-date pub-type="epub"><day>7</day><month>4</month><year>2010</year></pub-date><pub-date pub-type="ppub"><day>15</day><month>7</month><year>2010</year></pub-date><pub-date pub-type="pmc-release"><day>15</day><month>7</month><year>2011</year></pub-date><volume>25</volume><issue>11</issue><fpage>2431</fpage><lpage>2435</lpage><permissions><copyright-statement>© 2010 Elsevier B.V. All rights reserved.</copyright-statement><copyright-year>2010</copyright-year></permissions><abstract><p id="P1">We demonstrate a fluorescence-based, label-free detection scheme that reports the presence of Hg(II) ion using photon upconverting nanoparticles. A single-stranded DNA containing a number of thymine bases to be used as the Hg<sup>2+</sup>-capturing element is covalently attached to the photon upconverting NaYF<sub>4</sub>:Yb<sup>3+</sup>,Tm<sup>3+</sup> nanoparticles. Under the illumination of 980 nm laser, energy transfer takes place between the NaYF<sub>4</sub>:Yb<sup>3+</sup>,Tm<sup>3+</sup> nanoparticles as the donor and SYBR green I, a DNA intercalating dye, as the acceptor. By monitoring the ratio of the acceptor emission to the donor emission, we can quantitatively detect the presence of the mercuric ions with a directly observed detection limit of 0.06 nM. The remarkably high signal-to-noise ratio of photon upconverting particles leads to very high sensitivity and specificity without the need of fluorophore labeling. The sensor also does not suffer from photobleaching.</p></abstract><kwd-group><kwd>Photon upconversion</kwd><kwd>label-free detection</kwd><kwd>mercury ion</kwd><kwd>oligonucleotide</kwd><kwd>energy transfer</kwd></kwd-group><contract-num rid="RR1">P20 RR016480-09
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