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<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">Metal-Containing Polystyrene Beads as Standards for Mass Cytometry</title><author><name sortKey="Abdelrahman, Ahmed I" sort="Abdelrahman, Ahmed I" uniqKey="Abdelrahman A" first="Ahmed I." last="Abdelrahman">Ahmed I. Abdelrahman</name></author><author><name sortKey="Ornatsky, Olga" sort="Ornatsky, Olga" uniqKey="Ornatsky O" first="Olga" last="Ornatsky">Olga Ornatsky</name></author><author><name sortKey="Bandura, Dmitry" sort="Bandura, Dmitry" uniqKey="Bandura D" first="Dmitry" last="Bandura">Dmitry Bandura</name></author><author><name sortKey="Baranov, Vladimir" sort="Baranov, Vladimir" uniqKey="Baranov V" first="Vladimir" last="Baranov">Vladimir Baranov</name></author><author><name sortKey="Kinach, Robert" sort="Kinach, Robert" uniqKey="Kinach R" first="Robert" last="Kinach">Robert Kinach</name></author><author><name sortKey="Dai, Sheng" sort="Dai, Sheng" uniqKey="Dai S" first="Sheng" last="Dai">Sheng Dai</name></author><author><name sortKey="Thickett, Stuart C" sort="Thickett, Stuart C" uniqKey="Thickett S" first="Stuart C." last="Thickett">Stuart C. Thickett</name></author><author><name sortKey="Tanner, Scott" sort="Tanner, Scott" uniqKey="Tanner S" first="Scott" last="Tanner">Scott Tanner</name></author><author><name sortKey="Winnik, Mitchell A" sort="Winnik, Mitchell A" uniqKey="Winnik M" first="Mitchell A." last="Winnik">Mitchell A. Winnik</name></author></titleStmt><publicationStmt><idno type="wicri:source">PMC</idno><idno type="pmid">20390041</idno><idno type="pmc">2852877</idno><idno type="url">http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2852877</idno><idno type="RBID">PMC:2852877</idno><idno type="doi">10.1039/b921770c</idno><date when="2010">2010</date><idno type="wicri:Area/Pmc/Corpus">000230</idno><idno type="wicri:explorRef" wicri:stream="Pmc" wicri:step="Corpus" wicri:corpus="PMC">000230</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en" level="a" type="main">Metal-Containing Polystyrene Beads as Standards for Mass Cytometry</title><author><name sortKey="Abdelrahman, Ahmed I" sort="Abdelrahman, Ahmed I" uniqKey="Abdelrahman A" first="Ahmed I." last="Abdelrahman">Ahmed I. Abdelrahman</name></author><author><name sortKey="Ornatsky, Olga" sort="Ornatsky, Olga" uniqKey="Ornatsky O" first="Olga" last="Ornatsky">Olga Ornatsky</name></author><author><name sortKey="Bandura, Dmitry" sort="Bandura, Dmitry" uniqKey="Bandura D" first="Dmitry" last="Bandura">Dmitry Bandura</name></author><author><name sortKey="Baranov, Vladimir" sort="Baranov, Vladimir" uniqKey="Baranov V" first="Vladimir" last="Baranov">Vladimir Baranov</name></author><author><name sortKey="Kinach, Robert" sort="Kinach, Robert" uniqKey="Kinach R" first="Robert" last="Kinach">Robert Kinach</name></author><author><name sortKey="Dai, Sheng" sort="Dai, Sheng" uniqKey="Dai S" first="Sheng" last="Dai">Sheng Dai</name></author><author><name sortKey="Thickett, Stuart C" sort="Thickett, Stuart C" uniqKey="Thickett S" first="Stuart C." last="Thickett">Stuart C. Thickett</name></author><author><name sortKey="Tanner, Scott" sort="Tanner, Scott" uniqKey="Tanner S" first="Scott" last="Tanner">Scott Tanner</name></author><author><name sortKey="Winnik, Mitchell A" sort="Winnik, Mitchell A" uniqKey="Winnik M" first="Mitchell A." last="Winnik">Mitchell A. Winnik</name></author></analytic><series><title level="j">Journal of analytical atomic spectrometry</title><idno type="ISSN">0267-9477</idno><imprint><date when="2010">2010</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en"><p id="P1">We examine the suitability of metal-containing polystyrene beads for the calibration of a mass cytometer instrument, a single particle analyser based on an inductively coupled plasma ion source and a time of flight mass spectrometer. These metal-containing beads are also verified for their use as internal standards for this instrument. These beads were synthesized by multiple-stage dispersion polymerization with acrylic acid as a comonomer. Acrylic acid acts as a ligand to anchor the metal ions within the interior of the beads. Mass cytometry enabled the bead-by-bead measurement of the metal-content and determination of the metal-content distribution. Beads synthesized by dispersion polymerization that involved three stages were shown to have narrower bead-to-bead variation in their lanthanide content than beads synthesized by 2-stage dispersion polymerization. The beads exhibited insignificant release of their lanthanide content to aqueous solutions of different pHs over a period of six months. When mixed with KG1a or U937 cell lines, metal-containing polymer beads were shown not to affect the mass cytometry response to the metal content of element-tagged antibodies specifically attached to these cells.</p></div></front></TEI><pmc article-type="research-article" xml:lang="EN"><pmc-comment>The publisher of this article does not allow downloading of the full text in XML form.</pmc-comment>
<pmc-dir>properties manuscript</pmc-dir>
<front><journal-meta><journal-id journal-id-type="nlm-journal-id">8608254</journal-id><journal-id journal-id-type="pubmed-jr-id">34523</journal-id><journal-id journal-id-type="nlm-ta">J Anal At Spectrom</journal-id><journal-title>Journal of analytical atomic spectrometry</journal-title><issn pub-type="ppub">0267-9477</issn></journal-meta><article-meta><article-id pub-id-type="pmid">20390041</article-id><article-id pub-id-type="pmc">2852877</article-id><article-id pub-id-type="doi">10.1039/b921770c</article-id><article-id pub-id-type="manuscript">NIHMS178717</article-id><article-categories><subj-group subj-group-type="heading"><subject>Article</subject></subj-group></article-categories><title-group><article-title>Metal-Containing Polystyrene Beads as Standards for Mass Cytometry</article-title></title-group><contrib-group><contrib contrib-type="author"><name><surname>Abdelrahman</surname><given-names>Ahmed I.</given-names></name></contrib><contrib contrib-type="author"><name><surname>Ornatsky</surname><given-names>Olga</given-names></name></contrib><contrib contrib-type="author"><name><surname>Bandura</surname><given-names>Dmitry</given-names></name></contrib><contrib contrib-type="author" corresp="yes"><name><surname>Baranov</surname><given-names>Vladimir</given-names></name><email>vladimir.baranov@utoronto.ca</email></contrib><contrib contrib-type="author"><name><surname>Kinach</surname><given-names>Robert</given-names></name></contrib><contrib contrib-type="author"><name><surname>Dai</surname><given-names>Sheng</given-names></name></contrib><contrib contrib-type="author"><name><surname>Thickett</surname><given-names>Stuart C.</given-names></name></contrib><contrib contrib-type="author"><name><surname>Tanner</surname><given-names>Scott</given-names></name></contrib><contrib contrib-type="author" corresp="yes"><name><surname>Winnik</surname><given-names>Mitchell A.</given-names></name><email>mwinnik@chem.utoronto.ca</email></contrib><aff id="A1">Department of Chemistry, University of Toronto, 80 St George Street Toronto ON M5S3H6, Canada</aff></contrib-group><pub-date pub-type="nihms-submitted"><day>22</day><month>2</month><year>2010</year></pub-date><pub-date pub-type="ppub"><year>2010</year></pub-date><pub-date pub-type="pmc-release"><day>1</day><month>1</month><year>2011</year></pub-date><volume>25</volume><issue>3</issue><fpage>260</fpage><lpage>268</lpage><abstract><p id="P1">We examine the suitability of metal-containing polystyrene beads for the calibration of a mass cytometer instrument, a single particle analyser based on an inductively coupled plasma ion source and a time of flight mass spectrometer. These metal-containing beads are also verified for their use as internal standards for this instrument. These beads were synthesized by multiple-stage dispersion polymerization with acrylic acid as a comonomer. Acrylic acid acts as a ligand to anchor the metal ions within the interior of the beads. Mass cytometry enabled the bead-by-bead measurement of the metal-content and determination of the metal-content distribution. Beads synthesized by dispersion polymerization that involved three stages were shown to have narrower bead-to-bead variation in their lanthanide content than beads synthesized by 2-stage dispersion polymerization. The beads exhibited insignificant release of their lanthanide content to aqueous solutions of different pHs over a period of six months. When mixed with KG1a or U937 cell lines, metal-containing polymer beads were shown not to affect the mass cytometry response to the metal content of element-tagged antibodies specifically attached to these cells.</p></abstract><contract-num rid="GM1">R01 GM076127-04
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