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<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">Element-tagged immunoassay with ICP-MS detection: evaluation and comparison to conventional immunoassays</title>
<author><name sortKey="Razumienko, Eva" sort="Razumienko, Eva" uniqKey="Razumienko E" first="Eva" last="Razumienko">Eva Razumienko</name>
<affiliation><nlm:aff id="A1">Chemistry Department, University of Toronto, 80 St. George Street, Toronto, Ontario, Canada M5S 3H6</nlm:aff>
</affiliation>
</author>
<author><name sortKey="Ornatsky, Olga" sort="Ornatsky, Olga" uniqKey="Ornatsky O" first="Olga" last="Ornatsky">Olga Ornatsky</name>
<affiliation><nlm:aff id="A1">Chemistry Department, University of Toronto, 80 St. George Street, Toronto, Ontario, Canada M5S 3H6</nlm:aff>
</affiliation>
</author>
<author><name sortKey="Kinach, Robert" sort="Kinach, Robert" uniqKey="Kinach R" first="Robert" last="Kinach">Robert Kinach</name>
<affiliation><nlm:aff id="A1">Chemistry Department, University of Toronto, 80 St. George Street, Toronto, Ontario, Canada M5S 3H6</nlm:aff>
</affiliation>
</author>
<author><name sortKey="Milyavsky, Michael" sort="Milyavsky, Michael" uniqKey="Milyavsky M" first="Michael" last="Milyavsky">Michael Milyavsky</name>
<affiliation><nlm:aff id="A2">Division of Cellular and Molecular Biology, University Health Network, Toronto, Ontario M5G2M9, Canada.</nlm:aff>
</affiliation>
</author>
<author><name sortKey="Lechman, Eric" sort="Lechman, Eric" uniqKey="Lechman E" first="Eric" last="Lechman">Eric Lechman</name>
<affiliation><nlm:aff id="A2">Division of Cellular and Molecular Biology, University Health Network, Toronto, Ontario M5G2M9, Canada.</nlm:aff>
</affiliation>
</author>
<author><name sortKey="Baranov, Vladimir" sort="Baranov, Vladimir" uniqKey="Baranov V" first="Vladimir" last="Baranov">Vladimir Baranov</name>
<affiliation><nlm:aff id="A2">Division of Cellular and Molecular Biology, University Health Network, Toronto, Ontario M5G2M9, Canada.</nlm:aff>
</affiliation>
</author>
<author><name sortKey="Winnik, Mitchell A" sort="Winnik, Mitchell A" uniqKey="Winnik M" first="Mitchell A." last="Winnik">Mitchell A. Winnik</name>
<affiliation><nlm:aff id="A1">Chemistry Department, University of Toronto, 80 St. George Street, Toronto, Ontario, Canada M5S 3H6</nlm:aff>
</affiliation>
</author>
<author><name sortKey="Tanner, Scott D" sort="Tanner, Scott D" uniqKey="Tanner S" first="Scott D." last="Tanner">Scott D. Tanner</name>
<affiliation><nlm:aff id="A1">Chemistry Department, University of Toronto, 80 St. George Street, Toronto, Ontario, Canada M5S 3H6</nlm:aff>
</affiliation>
</author>
</titleStmt>
<publicationStmt><idno type="wicri:source">PMC</idno>
<idno type="pmid">18456275</idno>
<idno type="pmc">2583136</idno>
<idno type="url">http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2583136</idno>
<idno type="RBID">PMC:2583136</idno>
<idno type="doi">10.1016/j.jim.2008.03.011</idno>
<date when="2008">2008</date>
<idno type="wicri:Area/Pmc/Corpus">000219</idno>
<idno type="wicri:explorRef" wicri:stream="Pmc" wicri:step="Corpus" wicri:corpus="PMC">000219</idno>
</publicationStmt>
<sourceDesc><biblStruct><analytic><title xml:lang="en" level="a" type="main">Element-tagged immunoassay with ICP-MS detection: evaluation and comparison to conventional immunoassays</title>
<author><name sortKey="Razumienko, Eva" sort="Razumienko, Eva" uniqKey="Razumienko E" first="Eva" last="Razumienko">Eva Razumienko</name>
<affiliation><nlm:aff id="A1">Chemistry Department, University of Toronto, 80 St. George Street, Toronto, Ontario, Canada M5S 3H6</nlm:aff>
</affiliation>
</author>
<author><name sortKey="Ornatsky, Olga" sort="Ornatsky, Olga" uniqKey="Ornatsky O" first="Olga" last="Ornatsky">Olga Ornatsky</name>
<affiliation><nlm:aff id="A1">Chemistry Department, University of Toronto, 80 St. George Street, Toronto, Ontario, Canada M5S 3H6</nlm:aff>
</affiliation>
</author>
<author><name sortKey="Kinach, Robert" sort="Kinach, Robert" uniqKey="Kinach R" first="Robert" last="Kinach">Robert Kinach</name>
<affiliation><nlm:aff id="A1">Chemistry Department, University of Toronto, 80 St. George Street, Toronto, Ontario, Canada M5S 3H6</nlm:aff>
</affiliation>
</author>
<author><name sortKey="Milyavsky, Michael" sort="Milyavsky, Michael" uniqKey="Milyavsky M" first="Michael" last="Milyavsky">Michael Milyavsky</name>
<affiliation><nlm:aff id="A2">Division of Cellular and Molecular Biology, University Health Network, Toronto, Ontario M5G2M9, Canada.</nlm:aff>
</affiliation>
</author>
<author><name sortKey="Lechman, Eric" sort="Lechman, Eric" uniqKey="Lechman E" first="Eric" last="Lechman">Eric Lechman</name>
<affiliation><nlm:aff id="A2">Division of Cellular and Molecular Biology, University Health Network, Toronto, Ontario M5G2M9, Canada.</nlm:aff>
</affiliation>
</author>
<author><name sortKey="Baranov, Vladimir" sort="Baranov, Vladimir" uniqKey="Baranov V" first="Vladimir" last="Baranov">Vladimir Baranov</name>
<affiliation><nlm:aff id="A2">Division of Cellular and Molecular Biology, University Health Network, Toronto, Ontario M5G2M9, Canada.</nlm:aff>
</affiliation>
</author>
<author><name sortKey="Winnik, Mitchell A" sort="Winnik, Mitchell A" uniqKey="Winnik M" first="Mitchell A." last="Winnik">Mitchell A. Winnik</name>
<affiliation><nlm:aff id="A1">Chemistry Department, University of Toronto, 80 St. George Street, Toronto, Ontario, Canada M5S 3H6</nlm:aff>
</affiliation>
</author>
<author><name sortKey="Tanner, Scott D" sort="Tanner, Scott D" uniqKey="Tanner S" first="Scott D." last="Tanner">Scott D. Tanner</name>
<affiliation><nlm:aff id="A1">Chemistry Department, University of Toronto, 80 St. George Street, Toronto, Ontario, Canada M5S 3H6</nlm:aff>
</affiliation>
</author>
</analytic>
<series><title level="j">Journal of immunological methods</title>
<idno type="ISSN">0022-1759</idno>
<imprint><date when="2008">2008</date>
</imprint>
</series>
</biblStruct>
</sourceDesc>
</fileDesc>
<profileDesc><textClass></textClass>
</profileDesc>
</teiHeader>
<front><div type="abstract" xml:lang="en"><p id="P1">We have investigated the possibility of using element-tagged antibodies for protein detection and quantification in microplate format using Inductively Coupled Plasma Mass Spectrometry (ICP-MS), and compared the results to conventional immunoassays, such as Enzyme-Linked Immunosorbent Assay (ELISA) and Western blotting. The technique was further employed to detect low levels and measure DNA-binding activity of transcription factor p53 in leukemia cell lysates through its interaction with immobilized oligonucleotides and recognition by element-tagged antibodies. The advantages of ICP-MS detection for routine performance of immunoassays include increased sensitivity, wide dynamic range, minimal interference from complex matrices, and high throughput. Our approach advances the ICP-MS technology and demonstrates its applicability to proteomic studies through the use of antibodies directly labeled with polymer tags bearing multiple atoms of lanthanides. Development of this novel methodology will enable fast and quantitative identification of multiple analytes in a single well.</p>
</div>
</front>
</TEI>
<pmc article-type="research-article" xml:lang="EN"><pmc-comment>The publisher of this article does not allow downloading of the full text in XML form.</pmc-comment>
<pmc-dir>properties manuscript</pmc-dir>
<front><journal-meta><journal-id journal-id-type="nlm-journal-id">1305440</journal-id>
<journal-id journal-id-type="pubmed-jr-id">4818</journal-id>
<journal-id journal-id-type="nlm-ta">J Immunol Methods</journal-id>
<journal-title>Journal of immunological methods</journal-title>
<issn pub-type="ppub">0022-1759</issn>
</journal-meta>
<article-meta><article-id pub-id-type="pmid">18456275</article-id>
<article-id pub-id-type="pmc">2583136</article-id>
<article-id pub-id-type="manuscript">NIHMS55834</article-id>
<article-id pub-id-type="doi">10.1016/j.jim.2008.03.011</article-id>
<article-categories><subj-group subj-group-type="heading"><subject>Article</subject>
</subj-group>
</article-categories>
<title-group><article-title>Element-tagged immunoassay with ICP-MS detection: evaluation and comparison to conventional immunoassays</article-title>
</title-group>
<contrib-group><contrib contrib-type="author"><name><surname>Razumienko</surname>
<given-names>Eva</given-names>
</name>
<xref ref-type="aff" rid="A1">1</xref>
</contrib>
<contrib contrib-type="author"><name><surname>Ornatsky</surname>
<given-names>Olga</given-names>
</name>
<xref ref-type="aff" rid="A1">1</xref>
<xref ref-type="corresp" rid="cor1">*</xref>
</contrib>
<contrib contrib-type="author"><name><surname>Kinach</surname>
<given-names>Robert</given-names>
</name>
<xref ref-type="aff" rid="A1">1</xref>
</contrib>
<contrib contrib-type="author"><name><surname>Milyavsky</surname>
<given-names>Michael</given-names>
</name>
<xref ref-type="aff" rid="A2">2</xref>
</contrib>
<contrib contrib-type="author"><name><surname>Lechman</surname>
<given-names>Eric</given-names>
</name>
<xref ref-type="aff" rid="A2">2</xref>
</contrib>
<contrib contrib-type="author"><name><surname>Baranov</surname>
<given-names>Vladimir</given-names>
</name>
<xref ref-type="aff" rid="A2">2</xref>
</contrib>
<contrib contrib-type="author"><name><surname>Winnik</surname>
<given-names>Mitchell A.</given-names>
</name>
<xref ref-type="aff" rid="A1">1</xref>
</contrib>
<contrib contrib-type="author"><name><surname>Tanner</surname>
<given-names>Scott D.</given-names>
</name>
<xref ref-type="aff" rid="A1">1</xref>
</contrib>
</contrib-group>
<aff id="A1"><label>1</label>
Chemistry Department, University of Toronto, 80 St. George Street, Toronto, Ontario, Canada M5S 3H6</aff>
<aff id="A2"><label>2</label>
Division of Cellular and Molecular Biology, University Health Network, Toronto, Ontario M5G2M9, Canada.</aff>
<author-notes><corresp id="cor1"><label>*</label>
Corresponding author. Chemistry Department, University of Toronto, 80 St. George st. Room LM18, Toronto, ON M5S 3H6, Canada, Tel: 416 946 84 20, Fax: 416 978 8775, E-mail address: <email>olga.ornatsky@utoronto.ca</email>
</corresp>
</author-notes>
<pub-date pub-type="nihms-submitted"><day>29</day>
<month>7</month>
<year>2008</year>
</pub-date>
<pub-date pub-type="epub"><day>22</day>
<month>4</month>
<year>2008</year>
</pub-date>
<pub-date pub-type="ppub"><day>20</day>
<month>7</month>
<year>2008</year>
</pub-date>
<pub-date pub-type="pmc-release"><day>20</day>
<month>7</month>
<year>2009</year>
</pub-date>
<volume>336</volume>
<issue>1</issue>
<fpage>56</fpage>
<lpage>63</lpage>
<abstract><p id="P1">We have investigated the possibility of using element-tagged antibodies for protein detection and quantification in microplate format using Inductively Coupled Plasma Mass Spectrometry (ICP-MS), and compared the results to conventional immunoassays, such as Enzyme-Linked Immunosorbent Assay (ELISA) and Western blotting. The technique was further employed to detect low levels and measure DNA-binding activity of transcription factor p53 in leukemia cell lysates through its interaction with immobilized oligonucleotides and recognition by element-tagged antibodies. The advantages of ICP-MS detection for routine performance of immunoassays include increased sensitivity, wide dynamic range, minimal interference from complex matrices, and high throughput. Our approach advances the ICP-MS technology and demonstrates its applicability to proteomic studies through the use of antibodies directly labeled with polymer tags bearing multiple atoms of lanthanides. Development of this novel methodology will enable fast and quantitative identification of multiple analytes in a single well.</p>
</abstract>
<kwd-group><kwd>ELISA</kwd>
<kwd>ICP-MS</kwd>
<kwd>element-tagged antibody</kwd>
<kwd>lanthanides</kwd>
<kwd>growth factors</kwd>
<kwd>transcription factor p53</kwd>
</kwd-group>
<contract-num rid="GM1">R01 GM076127-02</contract-num>
<contract-num rid="GM1">R01 GM076127-01A1</contract-num>
<contract-sponsor id="GM1">National Institute of General Medical Sciences : NIGMS</contract-sponsor>
</article-meta>
</front>
</pmc>
</record>
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