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<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">NIR-responsive silica-coated NaYbF<sub>4</sub>:Er/Tm/Ho upconversion fluorescent nanoparticles with tunable emission colors and their applications in immunolabeling and fluorescent imaging of cancer cells</title><author><name sortKey="Wang, Meng" sort="Wang, Meng" uniqKey="Wang M" first="Meng" last="Wang">Meng Wang</name><affiliation><nlm:aff id="A1">Department of Chemistry, Northeastern University, Shenyang, 110004, P. R. China</nlm:aff></affiliation></author><author><name sortKey="Mi, Congcong" sort="Mi, Congcong" uniqKey="Mi C" first="Congcong" last="Mi">Congcong Mi</name><affiliation><nlm:aff id="A1">Department of Chemistry, Northeastern University, Shenyang, 110004, P. R. China</nlm:aff></affiliation></author><author><name sortKey="Zhang, Yixin" sort="Zhang, Yixin" uniqKey="Zhang Y" first="Yixin" last="Zhang">Yixin Zhang</name><affiliation><nlm:aff id="A1">Department of Chemistry, Northeastern University, Shenyang, 110004, P. R. China</nlm:aff></affiliation></author><author><name sortKey="Liu, Jinling" sort="Liu, Jinling" uniqKey="Liu J" first="Jinling" last="Liu">Jinling Liu</name><affiliation><nlm:aff id="A1">Department of Chemistry, Northeastern University, Shenyang, 110004, P. R. China</nlm:aff></affiliation></author><author><name sortKey="Li, Feng" sort="Li, Feng" uniqKey="Li F" first="Feng" last="Li">Feng Li</name><affiliation><nlm:aff id="A1">Department of Chemistry, Northeastern University, Shenyang, 110004, P. R. China</nlm:aff></affiliation></author><author><name sortKey="Mao, Chuanbin" sort="Mao, Chuanbin" uniqKey="Mao C" first="Chuanbin" last="Mao">Chuanbin Mao</name><affiliation><nlm:aff id="A2">Department of Chemistry & Biochemistry, University of Oklahoma. 620 Parrington Oval, Room 208, Norman, Oklahoma 73019, USA</nlm:aff></affiliation></author><author><name sortKey="Xu, Shukun" sort="Xu, Shukun" uniqKey="Xu S" first="Shukun" last="Xu">Shukun Xu</name><affiliation><nlm:aff id="A1">Department of Chemistry, Northeastern University, Shenyang, 110004, P. R. China</nlm:aff></affiliation></author></titleStmt><publicationStmt><idno type="wicri:source">PMC</idno><idno type="pmid">20160878</idno><idno type="pmc">2772074</idno><idno type="url">http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2772074</idno><idno type="RBID">PMC:2772074</idno><idno type="doi">10.1021/jp906394z</idno><date when="2009">2009</date><idno type="wicri:Area/Pmc/Corpus">000218</idno><idno type="wicri:explorRef" wicri:stream="Pmc" wicri:step="Corpus" wicri:corpus="PMC">000218</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en" level="a" type="main">NIR-responsive silica-coated NaYbF<sub>4</sub>:Er/Tm/Ho upconversion fluorescent nanoparticles with tunable emission colors and their applications in immunolabeling and fluorescent imaging of cancer cells</title><author><name sortKey="Wang, Meng" sort="Wang, Meng" uniqKey="Wang M" first="Meng" last="Wang">Meng Wang</name><affiliation><nlm:aff id="A1">Department of Chemistry, Northeastern University, Shenyang, 110004, P. R. China</nlm:aff></affiliation></author><author><name sortKey="Mi, Congcong" sort="Mi, Congcong" uniqKey="Mi C" first="Congcong" last="Mi">Congcong Mi</name><affiliation><nlm:aff id="A1">Department of Chemistry, Northeastern University, Shenyang, 110004, P. R. China</nlm:aff></affiliation></author><author><name sortKey="Zhang, Yixin" sort="Zhang, Yixin" uniqKey="Zhang Y" first="Yixin" last="Zhang">Yixin Zhang</name><affiliation><nlm:aff id="A1">Department of Chemistry, Northeastern University, Shenyang, 110004, P. R. China</nlm:aff></affiliation></author><author><name sortKey="Liu, Jinling" sort="Liu, Jinling" uniqKey="Liu J" first="Jinling" last="Liu">Jinling Liu</name><affiliation><nlm:aff id="A1">Department of Chemistry, Northeastern University, Shenyang, 110004, P. R. China</nlm:aff></affiliation></author><author><name sortKey="Li, Feng" sort="Li, Feng" uniqKey="Li F" first="Feng" last="Li">Feng Li</name><affiliation><nlm:aff id="A1">Department of Chemistry, Northeastern University, Shenyang, 110004, P. R. China</nlm:aff></affiliation></author><author><name sortKey="Mao, Chuanbin" sort="Mao, Chuanbin" uniqKey="Mao C" first="Chuanbin" last="Mao">Chuanbin Mao</name><affiliation><nlm:aff id="A2">Department of Chemistry & Biochemistry, University of Oklahoma. 620 Parrington Oval, Room 208, Norman, Oklahoma 73019, USA</nlm:aff></affiliation></author><author><name sortKey="Xu, Shukun" sort="Xu, Shukun" uniqKey="Xu S" first="Shukun" last="Xu">Shukun Xu</name><affiliation><nlm:aff id="A1">Department of Chemistry, Northeastern University, Shenyang, 110004, P. R. China</nlm:aff></affiliation></author></analytic><series><title level="j">The journal of physical chemistry. C, Nanomaterials and interfaces</title><idno type="ISSN">1932-7447</idno><idno type="eISSN">1932-7455</idno><imprint><date when="2009">2009</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en"><p id="P3">NaYbF<sub>4</sub>: RE upconversion (UC) fluorescent nanoparticles (NPs) were synthesized with variable rare-earth dopants (RE= Er<sup>3+</sup>, Tm<sup>3+</sup>, or Ho<sup>3+</sup>, or a combination of these ions), from rare-earth stearate precursors in a water-ethanol-oleic acid system by using a two-phase solvothermal method. The NPs were shown to emit visible light such as orange, yellow, green, cyan, blue or pink light in response to near infrared (NIR) irradiation, and their emission colors could be simply tuned by changing either the co-dopant concentration or dopant species. The UC NPs were well-dispersed and spherical with an average size of 15~35 nm. They emitted strong UC fluorescence under the 980 nm NIR excitation. The effects of solvothermal reaction time and temperature on nanoparticle size and phase structure as well as UC fluorescence intensity were systematically studied. Water dispersibility was achieved by forming a silica coat on the surface of the UC NPs. After animo-functionalization, the silica-coated UC NPs were chemically conjugated with the rabbit anti-CEA8 antibody and then used as fluorescent biolabels for the immunolabeling and imaging of HeLa cells. The NIR-responsive multicolor visible light emission of these UC NPs will enable potential applications in biolabeling and multiplexed analysis because NIR light can penetrate tissue as deep as several inches and is safe to human body.</p></div></front></TEI><pmc article-type="research-article" xml:lang="EN"><pmc-comment>The publisher of this article does not allow downloading of the full text in XML form.</pmc-comment>
<pmc-dir>properties manuscript</pmc-dir>
<front><journal-meta><journal-id journal-id-type="nlm-journal-id">101299949</journal-id><journal-id journal-id-type="pubmed-jr-id">34291</journal-id><journal-id journal-id-type="nlm-ta">J Phys Chem C Nanomater Interfaces</journal-id><journal-title>The journal of physical chemistry. C, Nanomaterials and interfaces</journal-title><issn pub-type="ppub">1932-7447</issn><issn pub-type="epub">1932-7455</issn></journal-meta><article-meta><article-id pub-id-type="pmid">20160878</article-id><article-id pub-id-type="pmc">2772074</article-id><article-id pub-id-type="doi">10.1021/jp906394z</article-id><article-id pub-id-type="manuscript">NIHMS152017</article-id><article-categories><subj-group subj-group-type="heading"><subject>Article</subject></subj-group></article-categories><title-group><article-title>NIR-responsive silica-coated NaYbF<sub>4</sub>:Er/Tm/Ho upconversion fluorescent nanoparticles with tunable emission colors and their applications in immunolabeling and fluorescent imaging of cancer cells</article-title></title-group><contrib-group><contrib contrib-type="author"><name><surname>Wang</surname><given-names>Meng</given-names></name><xref ref-type="aff" rid="A1">a</xref></contrib><contrib contrib-type="author"><name><surname>Mi</surname><given-names>Congcong</given-names></name><xref ref-type="aff" rid="A1">a</xref></contrib><contrib contrib-type="author"><name><surname>Zhang</surname><given-names>Yixin</given-names></name><xref ref-type="aff" rid="A1">a</xref></contrib><contrib contrib-type="author"><name><surname>Liu</surname><given-names>Jinling</given-names></name><xref ref-type="aff" rid="A1">a</xref></contrib><contrib contrib-type="author"><name><surname>Li</surname><given-names>Feng</given-names></name><xref ref-type="aff" rid="A1">a</xref></contrib><contrib contrib-type="author"><name><surname>Mao</surname><given-names>Chuanbin</given-names></name><xref ref-type="aff" rid="A2">b</xref><xref ref-type="author-notes" rid="FN2">*</xref></contrib><contrib contrib-type="author"><name><surname>Xu</surname><given-names>Shukun</given-names></name><xref ref-type="aff" rid="A1">a</xref><xref ref-type="author-notes" rid="FN1">*</xref></contrib></contrib-group><aff id="A1"><label>a</label>Department of Chemistry, Northeastern University, Shenyang, 110004, P. R. China</aff><aff id="A2"><label>b</label>Department of Chemistry & Biochemistry, University of Oklahoma. 620 Parrington Oval, Room 208, Norman, Oklahoma 73019, USA</aff><author-notes><fn fn-type="corresp" id="FN1"><label>*</label><p id="P1">To whom correspondence should be addressed. Tel.: 86 24 83681343, <email>xushukun46@126.com</email> (S. K. Xu)</p></fn><fn fn-type="corresp" id="FN2"><label>*</label><p id="P2">To whom correspondence should be addressed. Tel: 1 405 325 4385, <email>cbmao@ou.edu</email> (C. B. Mao).</p></fn></author-notes><pub-date pub-type="nihms-submitted"><day>19</day><month>10</month><year>2009</year></pub-date><pub-date pub-type="ppub"><day>5</day><month>11</month><year>2009</year></pub-date><pub-date pub-type="pmc-release"><day>5</day><month>11</month><year>2010</year></pub-date><volume>113</volume><issue>44</issue><fpage>19021</fpage><lpage>19027</lpage><abstract><p id="P3">NaYbF<sub>4</sub>: RE upconversion (UC) fluorescent nanoparticles (NPs) were synthesized with variable rare-earth dopants (RE= Er<sup>3+</sup>, Tm<sup>3+</sup>, or Ho<sup>3+</sup>, or a combination of these ions), from rare-earth stearate precursors in a water-ethanol-oleic acid system by using a two-phase solvothermal method. The NPs were shown to emit visible light such as orange, yellow, green, cyan, blue or pink light in response to near infrared (NIR) irradiation, and their emission colors could be simply tuned by changing either the co-dopant concentration or dopant species. The UC NPs were well-dispersed and spherical with an average size of 15~35 nm. They emitted strong UC fluorescence under the 980 nm NIR excitation. The effects of solvothermal reaction time and temperature on nanoparticle size and phase structure as well as UC fluorescence intensity were systematically studied. Water dispersibility was achieved by forming a silica coat on the surface of the UC NPs. After animo-functionalization, the silica-coated UC NPs were chemically conjugated with the rabbit anti-CEA8 antibody and then used as fluorescent biolabels for the immunolabeling and imaging of HeLa cells. The NIR-responsive multicolor visible light emission of these UC NPs will enable potential applications in biolabeling and multiplexed analysis because NIR light can penetrate tissue as deep as several inches and is safe to human body.</p></abstract><contract-num rid="EB1">R21 EB009909-01A1
||EB</contract-num><contract-num rid="AR1">R03 AR056848-01
||AR</contract-num><contract-num rid="HL1">R01 HL092526-01A2
||HL</contract-num><contract-sponsor id="EB1">National Institute of Biomedical Imaging and Bioengineering : NIBIB</contract-sponsor><contract-sponsor id="AR1">National Institute of Arthritis and Musculoskeletal and Skin Diseases : NIAMS</contract-sponsor><contract-sponsor id="HL1">National Heart, Lung, and Blood Institute : NHLBI</contract-sponsor></article-meta></front></pmc></record>Pour manipuler ce document sous Unix (Dilib)
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