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<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">A comparative study of the effect of cholesterol on bicelle model membranes using X-band and Q-band EPR spectroscopy</title><author><name sortKey="Ghimire, Harishchandra" sort="Ghimire, Harishchandra" uniqKey="Ghimire H" first="Harishchandra" last="Ghimire">Harishchandra Ghimire</name></author><author><name sortKey="Inbaraj, Johnson J" sort="Inbaraj, Johnson J" uniqKey="Inbaraj J" first="Johnson J." last="Inbaraj">Johnson J. Inbaraj</name></author><author><name sortKey="Lorigan, Gary A" sort="Lorigan, Gary A" uniqKey="Lorigan G" first="Gary A." last="Lorigan">Gary A. Lorigan</name></author></titleStmt><publicationStmt><idno type="wicri:source">PMC</idno><idno type="pmid">19501076</idno><idno type="pmc">2719848</idno><idno type="url">http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2719848</idno><idno type="RBID">PMC:2719848</idno><idno type="doi">10.1016/j.chemphyslip.2009.05.003</idno><date when="2009">2009</date><idno type="wicri:Area/Pmc/Corpus">000212</idno><idno type="wicri:explorRef" wicri:stream="Pmc" wicri:step="Corpus" wicri:corpus="PMC">000212</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en" level="a" type="main">A comparative study of the effect of cholesterol on bicelle model membranes using X-band and Q-band EPR spectroscopy</title><author><name sortKey="Ghimire, Harishchandra" sort="Ghimire, Harishchandra" uniqKey="Ghimire H" first="Harishchandra" last="Ghimire">Harishchandra Ghimire</name></author><author><name sortKey="Inbaraj, Johnson J" sort="Inbaraj, Johnson J" uniqKey="Inbaraj J" first="Johnson J." last="Inbaraj">Johnson J. Inbaraj</name></author><author><name sortKey="Lorigan, Gary A" sort="Lorigan, Gary A" uniqKey="Lorigan G" first="Gary A." last="Lorigan">Gary A. Lorigan</name></author></analytic><series><title level="j">Chemistry and physics of lipids</title><idno type="ISSN">0009-3084</idno><idno type="eISSN">1873-2941</idno><imprint><date when="2009">2009</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en"><p id="P2">X-band and Q-band electron paramagnetic resonance (EPR) spectroscopic techniques were used to investigate the structure and dynamics of cholesterol containing phospholipid bicelles based upon molecular order parameters (S<sub>mol</sub>), orientational dependent hyperfine splittings and line shape analysis of the corresponding EPR spectra. The nitroxide spin label 3-β-doxyl-5-α-cholestane (cholestane) was incorporated into DMPC/DHPC bicelles to report the alignment of bicelles in the static magnetic field. The influence of cholesterol on aligned phospholipid bicelles in terms of ordering, the ease of alignment, phase transition temperature have been studied comparatively at X-band and Q-band. At a magnetic field of 1.25 T (Q-band), bicelles with 20 mol% cholesterol aligned at a much lower temperature (313 K), when compared to 318 K at a 0.35 T field strength for X-band, showed better hyperfine splitting values (18.29 G at X-band vs. 18.55 G at Q-band for perpendicular alignment and 8.25 G at X-band vs. 7.83 G at Q-band for the parallel alignment at 318 K) and have greater molecular order parameters (0.76 at X-band vs. 0.86 at Q-band at 318 K). Increasing cholesterol content increased the bicelle ordering, the bicelle-alignment temperature and the gel to liquid crystalline phase transition temperature. We observed that Q-band is more effective than X-band for studying aligned bicelles, because it yielded a higher ordered bicelle system for EPR spectroscopic studies.</p></div></front></TEI><pmc article-type="research-article" xml:lang="EN"><pmc-comment>The publisher of this article does not allow downloading of the full text in XML form.</pmc-comment>
<pmc-dir>properties manuscript</pmc-dir>
<front><journal-meta><journal-id journal-id-type="nlm-journal-id">0067206</journal-id><journal-id journal-id-type="pubmed-jr-id">2910</journal-id><journal-id journal-id-type="nlm-ta">Chem Phys Lipids</journal-id><journal-title>Chemistry and physics of lipids</journal-title><issn pub-type="ppub">0009-3084</issn><issn pub-type="epub">1873-2941</issn></journal-meta><article-meta><article-id pub-id-type="pmid">19501076</article-id><article-id pub-id-type="pmc">2719848</article-id><article-id pub-id-type="doi">10.1016/j.chemphyslip.2009.05.003</article-id><article-id pub-id-type="manuscript">NIHMS121623</article-id><article-categories><subj-group subj-group-type="heading"><subject>Article</subject></subj-group></article-categories><title-group><article-title>A comparative study of the effect of cholesterol on bicelle model membranes using X-band and Q-band EPR spectroscopy</article-title></title-group><contrib-group><contrib contrib-type="author"><name><surname>Ghimire</surname><given-names>Harishchandra</given-names></name></contrib><contrib contrib-type="author"><name><surname>Inbaraj</surname><given-names>Johnson J.</given-names></name></contrib><contrib contrib-type="author" corresp="yes"><name><surname>Lorigan</surname><given-names>Gary A.</given-names></name></contrib><aff id="A1">Department of Chemistry and Biochemistry, Miami University, Oxford, OH, 45056, Email:<email>garylorigan@muohio.edu</email></aff></contrib-group><pub-date pub-type="nihms-submitted"><day>14</day><month>7</month><year>2009</year></pub-date><pub-date pub-type="epub"><day>6</day><month>6</month><year>2009</year></pub-date><pub-date pub-type="ppub"><month>8</month><year>2009</year></pub-date><pub-date pub-type="pmc-release"><day>1</day><month>8</month><year>2010</year></pub-date><volume>160</volume><issue>2</issue><fpage>98</fpage><lpage>104</lpage><abstract><p id="P2">X-band and Q-band electron paramagnetic resonance (EPR) spectroscopic techniques were used to investigate the structure and dynamics of cholesterol containing phospholipid bicelles based upon molecular order parameters (S<sub>mol</sub>), orientational dependent hyperfine splittings and line shape analysis of the corresponding EPR spectra. The nitroxide spin label 3-β-doxyl-5-α-cholestane (cholestane) was incorporated into DMPC/DHPC bicelles to report the alignment of bicelles in the static magnetic field. The influence of cholesterol on aligned phospholipid bicelles in terms of ordering, the ease of alignment, phase transition temperature have been studied comparatively at X-band and Q-band. At a magnetic field of 1.25 T (Q-band), bicelles with 20 mol% cholesterol aligned at a much lower temperature (313 K), when compared to 318 K at a 0.35 T field strength for X-band, showed better hyperfine splitting values (18.29 G at X-band vs. 18.55 G at Q-band for perpendicular alignment and 8.25 G at X-band vs. 7.83 G at Q-band for the parallel alignment at 318 K) and have greater molecular order parameters (0.76 at X-band vs. 0.86 at Q-band at 318 K). Increasing cholesterol content increased the bicelle ordering, the bicelle-alignment temperature and the gel to liquid crystalline phase transition temperature. We observed that Q-band is more effective than X-band for studying aligned bicelles, because it yielded a higher ordered bicelle system for EPR spectroscopic studies.</p></abstract><kwd-group><kwd>Phospholipids</kwd><kwd>X-band</kwd><kwd>Q-band</kwd><kwd>EPR</kwd><kwd>cholestane</kwd><kwd>molecular order parameter</kwd><kwd>hyperfine splitting</kwd></kwd-group><contract-num rid="GM1">R01 GM080542-01A2</contract-num><contract-sponsor id="GM1">National Institute of General Medical Sciences : NIGMS</contract-sponsor></article-meta></front></pmc></record>Pour manipuler ce document sous Unix (Dilib)
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