Regulation of BN115, a low-temperature-responsive gene from winter Brassica napus.
Identifieur interne : 000174 ( Pmc/Corpus ); précédent : 000173; suivant : 000175Regulation of BN115, a low-temperature-responsive gene from winter Brassica napus.
Auteurs : T C White ; D. Simmonds ; P. Donaldson ; J. SinghSource :
- Plant Physiology [ 0032-0889 ] ; 1994.
Abstract
The genomic clone for BN115, a low-temperature-responsive gene, was isolated from winter Brassica napus and its sequence was determined. A 1.2-kb fragment of the 5' regulatory region (from bp -1107 to +100) was fused to the beta-glucuronidase (GUS) reporter gene and BN115-promoted GUS expression was observed in green tissues of transgenic B. napus plants only after incubation at 2 degrees C. No expression was observed after incubation at 22 degrees C, either in the presence or the absence of ABA. Microprojectile bombardment of winter B. napus leaves with a BN115 promoter/GUS construct yielded similar results and was used to analyze a series of deletions from the 5' end of the promoter. Results obtained from transient expression studies showed that the low-temperature regulation of BN115 expression involves a possible enhancer region between bp -1107 and -802 and a second positive regulatory region located between bp -302 and -274. Deletion analyses and results from replacement with a truncated cauliflower mosaic virus 35S promoter suggest that the minimal size required for any maintenance of low-temperature GUS expression is a -300-bp fragment. Within this fragment are two 8-bp elements with the sequence TGGCCGAC, which are identical to those present in the positive regulatory region of the promoter of the homologous Arabidopsis cor15a gene and to a 5-bp core sequence in the low-temperature- and dehydration-responsive elements identified in the promoter regions of several cold-responsive Arabidopsis thaliana genes.
Url:
PubMed: 7824659
PubMed Central: 159614
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PMC:159614Le document en format XML
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<author><name sortKey="Simmonds, D" sort="Simmonds, D" uniqKey="Simmonds D" first="D" last="Simmonds">D. Simmonds</name>
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<author><name sortKey="Donaldson, P" sort="Donaldson, P" uniqKey="Donaldson P" first="P" last="Donaldson">P. Donaldson</name>
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<author><name sortKey="Singh, J" sort="Singh, J" uniqKey="Singh J" first="J" last="Singh">J. Singh</name>
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<series><title level="j">Plant Physiology</title>
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<front><div type="abstract" xml:lang="en"><p>The genomic clone for BN115, a low-temperature-responsive gene, was isolated from winter Brassica napus and its sequence was determined. A 1.2-kb fragment of the 5' regulatory region (from bp -1107 to +100) was fused to the beta-glucuronidase (GUS) reporter gene and BN115-promoted GUS expression was observed in green tissues of transgenic B. napus plants only after incubation at 2 degrees C. No expression was observed after incubation at 22 degrees C, either in the presence or the absence of ABA. Microprojectile bombardment of winter B. napus leaves with a BN115 promoter/GUS construct yielded similar results and was used to analyze a series of deletions from the 5' end of the promoter. Results obtained from transient expression studies showed that the low-temperature regulation of BN115 expression involves a possible enhancer region between bp -1107 and -802 and a second positive regulatory region located between bp -302 and -274. Deletion analyses and results from replacement with a truncated cauliflower mosaic virus 35S promoter suggest that the minimal size required for any maintenance of low-temperature GUS expression is a -300-bp fragment. Within this fragment are two 8-bp elements with the sequence TGGCCGAC, which are identical to those present in the positive regulatory region of the promoter of the homologous Arabidopsis cor15a gene and to a 5-bp core sequence in the low-temperature- and dehydration-responsive elements identified in the promoter regions of several cold-responsive Arabidopsis thaliana genes.</p>
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<front><journal-meta><journal-id journal-id-type="nlm-ta">Plant Physiol</journal-id>
<journal-title>Plant Physiology</journal-title>
<issn pub-type="ppub">0032-0889</issn>
<issn pub-type="epub">1532-2548</issn>
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<article-meta><article-id pub-id-type="pmid">7824659</article-id>
<article-id pub-id-type="pmc">159614</article-id>
<article-id pub-id-type="pii">106/3/917</article-id>
<article-categories><subj-group subj-group-type="heading"><subject>Research Article</subject>
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<title-group><article-title>Regulation of BN115, a low-temperature-responsive gene from winter Brassica napus.</article-title>
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<contrib-group><contrib contrib-type="author"><name><surname>White</surname>
<given-names>T C</given-names>
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<contrib contrib-type="author"><name><surname>Simmonds</surname>
<given-names>D</given-names>
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<contrib contrib-type="author"><name><surname>Donaldson</surname>
<given-names>P</given-names>
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<contrib contrib-type="author"><name><surname>Singh</surname>
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<aff>Plant Research Centre, Agriculture Canada, Ottawa, Ontario.</aff>
<pub-date pub-type="ppub"><month>11</month>
<year>1994</year>
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<volume>106</volume>
<issue>3</issue>
<fpage>917</fpage>
<lpage>928</lpage>
<abstract><p>The genomic clone for BN115, a low-temperature-responsive gene, was isolated from winter Brassica napus and its sequence was determined. A 1.2-kb fragment of the 5' regulatory region (from bp -1107 to +100) was fused to the beta-glucuronidase (GUS) reporter gene and BN115-promoted GUS expression was observed in green tissues of transgenic B. napus plants only after incubation at 2 degrees C. No expression was observed after incubation at 22 degrees C, either in the presence or the absence of ABA. Microprojectile bombardment of winter B. napus leaves with a BN115 promoter/GUS construct yielded similar results and was used to analyze a series of deletions from the 5' end of the promoter. Results obtained from transient expression studies showed that the low-temperature regulation of BN115 expression involves a possible enhancer region between bp -1107 and -802 and a second positive regulatory region located between bp -302 and -274. Deletion analyses and results from replacement with a truncated cauliflower mosaic virus 35S promoter suggest that the minimal size required for any maintenance of low-temperature GUS expression is a -300-bp fragment. Within this fragment are two 8-bp elements with the sequence TGGCCGAC, which are identical to those present in the positive regulatory region of the promoter of the homologous Arabidopsis cor15a gene and to a 5-bp core sequence in the low-temperature- and dehydration-responsive elements identified in the promoter regions of several cold-responsive Arabidopsis thaliana genes.</p>
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