Deep-UV biological imaging by lanthanide ion molecular protection
Identifieur interne : 000053 ( Pmc/Corpus ); précédent : 000052; suivant : 000054Deep-UV biological imaging by lanthanide ion molecular protection
Auteurs : Yasuaki Kumamoto ; Katsumasa Fujita ; Nicholas Isaac Smith ; Satoshi KawataSource :
- Biomedical Optics Express [ 2156-7085 ] ; 2015.
Abstract
Deep-UV (DUV) light is a sensitive probe for biological molecules such as nucleobases and aromatic amino acids due to specific absorption. However, the use of DUV light for imaging is limited because DUV can destroy or denature target molecules in a sample. Here we show that trivalent ions in the lanthanide group can suppress molecular photodegradation under DUV exposure, enabling a high signal-to-noise ratio and repetitive DUV imaging of nucleobases in cells. Underlying mechanisms of the photodegradation suppression can be excitation relaxation of the DUV-absorptive molecules due to energy transfer to the lanthanide ions, and/or avoiding ionization and reactions with surrounding molecules, including generation of reactive oxygen species, which can modify molecules that are otherwise transparent to DUV light. This approach, directly removing excited energy at the fundamental origin of cellular photodegradation, indicates an important first step towards the practical use of DUV imaging in a variety of biological applications.
Url:
DOI: 10.1364/BOE.7.000158
PubMed: 26819825
PubMed Central: 4722900
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PMC:4722900Le document en format XML
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<author><name sortKey="Kumamoto, Yasuaki" sort="Kumamoto, Yasuaki" uniqKey="Kumamoto Y" first="Yasuaki" last="Kumamoto">Yasuaki Kumamoto</name>
<affiliation><nlm:aff id="aff1">Department of Applied Physics, Osaka University, 2-1 Yamadaoka, Suita, Osaka 565-0871,<country country="JP">Japan</country>
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<affiliation><nlm:aff id="aff2">Near-field Nanophotonics Research Team, RIKEN, 2-1 Hirosawa, Wako, Saitama 351-0198,<country country="JP">Japan</country>
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<affiliation><nlm:aff id="aff4">Currently with the Department of Pathology and Cell Regulation, Kyoto Prefectural University of Medicine, 465 Kajii-cho Kawaramachi-Hirokoji, Kamigyo-ku, Kyoto 602-8566,<country country="JP">Japan</country>
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<author><name sortKey="Fujita, Katsumasa" sort="Fujita, Katsumasa" uniqKey="Fujita K" first="Katsumasa" last="Fujita">Katsumasa Fujita</name>
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<author><name sortKey="Smith, Nicholas Isaac" sort="Smith, Nicholas Isaac" uniqKey="Smith N" first="Nicholas Isaac" last="Smith">Nicholas Isaac Smith</name>
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<author><name sortKey="Kawata, Satoshi" sort="Kawata, Satoshi" uniqKey="Kawata S" first="Satoshi" last="Kawata">Satoshi Kawata</name>
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<affiliation><nlm:aff id="aff4">Currently with the Department of Pathology and Cell Regulation, Kyoto Prefectural University of Medicine, 465 Kajii-cho Kawaramachi-Hirokoji, Kamigyo-ku, Kyoto 602-8566,<country country="JP">Japan</country>
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<author><name sortKey="Fujita, Katsumasa" sort="Fujita, Katsumasa" uniqKey="Fujita K" first="Katsumasa" last="Fujita">Katsumasa Fujita</name>
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<series><title level="j">Biomedical Optics Express</title>
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<front><div type="abstract" xml:lang="en"><p>Deep-UV (DUV) light is a sensitive probe for biological molecules such as nucleobases and aromatic amino acids due to specific absorption. However, the use of DUV light for imaging is limited because DUV can destroy or denature target molecules in a sample. Here we show that trivalent ions in the lanthanide group can suppress molecular photodegradation under DUV exposure, enabling a high signal-to-noise ratio and repetitive DUV imaging of nucleobases in cells. Underlying mechanisms of the photodegradation suppression can be excitation relaxation of the DUV-absorptive molecules due to energy transfer to the lanthanide ions, and/or avoiding ionization and reactions with surrounding molecules, including generation of reactive oxygen species, which can modify molecules that are otherwise transparent to DUV light. This approach, directly removing excited energy at the fundamental origin of cellular photodegradation, indicates an important first step towards the practical use of DUV imaging in a variety of biological applications.</p>
</div>
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<pmc article-type="research-article"><pmc-comment>The publisher of this article does not allow downloading of the full text in XML form.</pmc-comment>
<front><journal-meta><journal-id journal-id-type="nlm-ta">Biomed Opt Express</journal-id>
<journal-id journal-id-type="iso-abbrev">Biomed Opt Express</journal-id>
<journal-id journal-id-type="publisher-id">BOE</journal-id>
<journal-title-group><journal-title>Biomedical Optics Express</journal-title>
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<issn pub-type="epub">2156-7085</issn>
<publisher><publisher-name>Optical Society of America</publisher-name>
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<article-id pub-id-type="publisher-id">253648</article-id>
<article-id pub-id-type="doi">10.1364/BOE.7.000158</article-id>
<article-categories><subj-group subj-group-type="heading"><subject>Article</subject>
</subj-group>
</article-categories>
<title-group><article-title>Deep-UV biological imaging by lanthanide ion molecular protection</article-title>
</title-group>
<contrib-group><contrib contrib-type="author"><name><surname>Kumamoto</surname>
<given-names>Yasuaki</given-names>
</name>
<xref ref-type="aff" rid="aff1"><sup>1</sup>
</xref>
<xref ref-type="aff" rid="aff2"><sup>2</sup>
</xref>
<xref ref-type="aff" rid="aff4"><sup>4</sup>
</xref>
</contrib>
<contrib contrib-type="author"><name><surname>Fujita</surname>
<given-names>Katsumasa</given-names>
</name>
<xref ref-type="aff" rid="aff1"><sup>1</sup>
</xref>
</contrib>
<contrib contrib-type="author"><name><surname>Smith</surname>
<given-names>Nicholas Isaac</given-names>
</name>
<xref ref-type="aff" rid="aff3"><sup>3</sup>
</xref>
</contrib>
<contrib contrib-type="author"><name><surname>Kawata</surname>
<given-names>Satoshi</given-names>
</name>
<xref ref-type="aff" rid="aff1"><sup>1</sup>
</xref>
<xref ref-type="aff" rid="aff2"><sup>2</sup>
</xref>
<xref ref-type="corresp" rid="cor1"><sup>*</sup>
</xref>
</contrib>
<aff id="aff1"><label>1</label>
Department of Applied Physics, Osaka University, 2-1 Yamadaoka, Suita, Osaka 565-0871,<country country="JP">Japan</country>
</aff>
<aff id="aff2"><label>2</label>
Near-field Nanophotonics Research Team, RIKEN, 2-1 Hirosawa, Wako, Saitama 351-0198,<country country="JP">Japan</country>
</aff>
<aff id="aff3"><label>3</label>
Immunology Frontier Research Center, Osaka University, 3-1 Yamadaoka, Suita, Osaka 565-0871,<country country="JP">Japan</country>
</aff>
<aff id="aff4"><label>4</label>
Currently with the Department of Pathology and Cell Regulation, Kyoto Prefectural University of Medicine, 465 Kajii-cho Kawaramachi-Hirokoji, Kamigyo-ku, Kyoto 602-8566,<country country="JP">Japan</country>
</aff>
</contrib-group>
<author-notes><corresp id="cor1"><label>*</label>
<email xlink:href="kawata@ap.eng.osaka-u.ac.jp">kawata@ap.eng.osaka-u.ac.jp</email>
</corresp>
</author-notes>
<pub-date pub-type="epub"><day>18</day>
<month>12</month>
<year>2015</year>
</pub-date>
<pub-date pub-type="collection"><day>01</day>
<month>1</month>
<year>2016</year>
</pub-date>
<pub-date pub-type="pmc-release"><day>18</day>
<month>12</month>
<year>2015</year>
</pub-date>
<pmc-comment> PMC Release delay is 0 months and 0 days and was based on the
. </pmc-comment>
<volume>7</volume>
<issue>1</issue>
<fpage>158</fpage>
<lpage>170</lpage>
<history><date date-type="received"><day>09</day>
<month>11</month>
<year>2015</year>
</date>
<date date-type="rev-recd"><day>08</day>
<month>12</month>
<year>2015</year>
</date>
<date date-type="accepted"><day>11</day>
<month>12</month>
<year>2015</year>
</date>
</history>
<permissions><copyright-statement>© 2015 Optical Society of America</copyright-statement>
<copyright-year>2015</copyright-year>
<copyright-holder>Optical Society of America</copyright-holder>
</permissions>
<abstract><p>Deep-UV (DUV) light is a sensitive probe for biological molecules such as nucleobases and aromatic amino acids due to specific absorption. However, the use of DUV light for imaging is limited because DUV can destroy or denature target molecules in a sample. Here we show that trivalent ions in the lanthanide group can suppress molecular photodegradation under DUV exposure, enabling a high signal-to-noise ratio and repetitive DUV imaging of nucleobases in cells. Underlying mechanisms of the photodegradation suppression can be excitation relaxation of the DUV-absorptive molecules due to energy transfer to the lanthanide ions, and/or avoiding ionization and reactions with surrounding molecules, including generation of reactive oxygen species, which can modify molecules that are otherwise transparent to DUV light. This approach, directly removing excited energy at the fundamental origin of cellular photodegradation, indicates an important first step towards the practical use of DUV imaging in a variety of biological applications.</p>
</abstract>
<kwd-group kwd-group-type="OCIS"><title>OCIS codes: </title>
<kwd>(300.6540) Spectroscopy, ultraviolet</kwd>
<kwd>(170.3880) Medical and biological imaging</kwd>
<kwd>(260.5130) Photochemistry</kwd>
</kwd-group>
<funding-group><award-group id="sp1"><funding-source>Japan Society for the Promotion of Science (JSPS)<named-content content-type="doi">10.13039/501100001691</named-content>
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